Development of high capacity lithium-manganese-rich cathode materials xLi2MnO3•(1-x)LiMn0.5Ni0.5O2 for lithium ion batteries
- Authors: Rapulenyane, Nomasonto
- Date: 2018
- Subjects: Lithium ion batteries , Electrochemistry Lithium cells
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/34766 , vital:33442
- Description: In this study, a facile synthesis method was developed to produce layered-layered cathode materials with the formula xLi2MnO3•(1-x)LiMO2 (M= Ni and Mn) referred to as lithium-manganese-rich materials for lithium ion batteries. The prepared materials displayed high capacity ≥200 mAh/g at a current density of 20 mA/g in the voltage range of 2.0 V to 4.8 V. In particular the cathode material prepared at pH 10.0 delivered a high initial discharge capacity of 266 mAh/g at 20 mA/g current density and maintained a discharge capacity ≥220 mAh/g at 50 mA/g after 50 cycles. The synthesis method was used to further investigate the effect of lithium ratio in the layered-layered material. Li1+xMn0.6Ni0.2O2, x= 0.2, 0.25, 0.3 and 0.4 cathode materials were produced respectively. The BET surface area analysis results showed that Li1.3Mn0.6Ni0.2O2 material had comparatively higher surface area to the other cathode materials and also delivered good electrochemical results. XPS showed that the cation distribution is affected by the increase in lithium ratio, the Mn4+ percentages decreased significantly with an increase in lithium ratio. All materials peaks deconvoluted into two peaks namely Mn4+ and Mn3+, Li1.3Mn0.6Ni0.2O2 had the highest percentages of the stable Mn4+ 70.8%. Further investigation focused on the effect of the sintering temperature on the structure and the electrochemical performance of Li1+xMn0.6Ni0.2O2, x= 0.25, 0.3 and 0.4 cathode materials. X-ray diffraction showed the same patterns for all cathode materials sintered at 700˚C, 800˚C and 900˚C. Rietveld refined results however, showed that the increase in the sintering temperature, results in a decrease in the Li2MnO3 component percentage in the layered structures. Scanning electron microscopy images further proved that the particle size increases with increasing temperature. The charge–discharge tests of coin cells demonstrated that the materials sintered at 800˚C delivered higher discharge capacities above 200 mAh/g at 20 mA/g current density when compared to the materials made at the lower temperatures. Lastly the cathode material prepared at pH 10.0 was further evaluated in a cell using lithium titanate oxide Li4Ti5O12 as anode material. The cells delivered an initial discharge capacity of 213 mAh/g at 20 mA/g within a voltage range 3.3V-0.5V. The coin cells developed in this work delivered good cycling performance.
- Full Text:
- Date Issued: 2018
- Authors: Rapulenyane, Nomasonto
- Date: 2018
- Subjects: Lithium ion batteries , Electrochemistry Lithium cells
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/34766 , vital:33442
- Description: In this study, a facile synthesis method was developed to produce layered-layered cathode materials with the formula xLi2MnO3•(1-x)LiMO2 (M= Ni and Mn) referred to as lithium-manganese-rich materials for lithium ion batteries. The prepared materials displayed high capacity ≥200 mAh/g at a current density of 20 mA/g in the voltage range of 2.0 V to 4.8 V. In particular the cathode material prepared at pH 10.0 delivered a high initial discharge capacity of 266 mAh/g at 20 mA/g current density and maintained a discharge capacity ≥220 mAh/g at 50 mA/g after 50 cycles. The synthesis method was used to further investigate the effect of lithium ratio in the layered-layered material. Li1+xMn0.6Ni0.2O2, x= 0.2, 0.25, 0.3 and 0.4 cathode materials were produced respectively. The BET surface area analysis results showed that Li1.3Mn0.6Ni0.2O2 material had comparatively higher surface area to the other cathode materials and also delivered good electrochemical results. XPS showed that the cation distribution is affected by the increase in lithium ratio, the Mn4+ percentages decreased significantly with an increase in lithium ratio. All materials peaks deconvoluted into two peaks namely Mn4+ and Mn3+, Li1.3Mn0.6Ni0.2O2 had the highest percentages of the stable Mn4+ 70.8%. Further investigation focused on the effect of the sintering temperature on the structure and the electrochemical performance of Li1+xMn0.6Ni0.2O2, x= 0.25, 0.3 and 0.4 cathode materials. X-ray diffraction showed the same patterns for all cathode materials sintered at 700˚C, 800˚C and 900˚C. Rietveld refined results however, showed that the increase in the sintering temperature, results in a decrease in the Li2MnO3 component percentage in the layered structures. Scanning electron microscopy images further proved that the particle size increases with increasing temperature. The charge–discharge tests of coin cells demonstrated that the materials sintered at 800˚C delivered higher discharge capacities above 200 mAh/g at 20 mA/g current density when compared to the materials made at the lower temperatures. Lastly the cathode material prepared at pH 10.0 was further evaluated in a cell using lithium titanate oxide Li4Ti5O12 as anode material. The cells delivered an initial discharge capacity of 213 mAh/g at 20 mA/g within a voltage range 3.3V-0.5V. The coin cells developed in this work delivered good cycling performance.
- Full Text:
- Date Issued: 2018
Application of hidden Markov models and their extensions to animal movement data
- Authors: Van Niekerk, Bracken
- Date: 2018
- Subjects: Markov processes Animal locomotion Time-series analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/23835 , vital:30624
- Description: Hidden Markov Models (HMMs) have become increasingly popular in animal movement studies as they provide a flexible modelling approach and take the correlation between successive observations into account. They can segment the movement paths into latent states, which can be considered as rough proxies for the behaviours of the animals. This study comprises of two sections, both involving the application of HMMs to large terrestrial mammal movement data. Usually step lengths representing the displacement distances between successive observations, turning angles measuring the tortuosity, or a bivariate input of both variables are used as inputs in the models. It has been found in the literature that the turning angle is either included in the modelling process or it is excluded without much justification for doing so. The first part of this study investigates the nfluence of the turning angle on the model output and resultant interpretations of the HMMs when modelling the trajectories of large terrestrial mammals in southern Africa. Results revealed at different time scales, and for both predator and herbivore species in this study, that the turning angle does not influence the state allocation of the HMMs, which is the main output in terms of interpreting the behaviours of the animals. It is thought in most cases that the inclusion of the turning angle overcomplicates the models unnecessarily without contributing any additional information in terms of the behavioural interpretations or improving the overall fit of the models. This was found for the variety of movements of the species under observation in this study. The second part of this study attempts to validate the state allocation of the HMMs fitted to eland trajectories in the Greater Addo Elephant National Park in the Eastern Cape, with the use of camera trap data. This presented a unique opportunity as this type of data is mainly used for abundance or capture-recapture studies, and the HMMs are rarely validated as the true behaviours of the animals are seldom known. Results revealed that the same diel patterns were detected by the HMMs that were shown by the classified camera trap data. Direct comparisons of the observations where the dates and times matched for the telemetry and camera trap data could be done in several rare instances, which revealed many similarities. Although it was not an ideal comparison, the camera trap data provided a rough validation of the state allocation of the HMMs used in the study.
- Full Text:
- Date Issued: 2018
- Authors: Van Niekerk, Bracken
- Date: 2018
- Subjects: Markov processes Animal locomotion Time-series analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/23835 , vital:30624
- Description: Hidden Markov Models (HMMs) have become increasingly popular in animal movement studies as they provide a flexible modelling approach and take the correlation between successive observations into account. They can segment the movement paths into latent states, which can be considered as rough proxies for the behaviours of the animals. This study comprises of two sections, both involving the application of HMMs to large terrestrial mammal movement data. Usually step lengths representing the displacement distances between successive observations, turning angles measuring the tortuosity, or a bivariate input of both variables are used as inputs in the models. It has been found in the literature that the turning angle is either included in the modelling process or it is excluded without much justification for doing so. The first part of this study investigates the nfluence of the turning angle on the model output and resultant interpretations of the HMMs when modelling the trajectories of large terrestrial mammals in southern Africa. Results revealed at different time scales, and for both predator and herbivore species in this study, that the turning angle does not influence the state allocation of the HMMs, which is the main output in terms of interpreting the behaviours of the animals. It is thought in most cases that the inclusion of the turning angle overcomplicates the models unnecessarily without contributing any additional information in terms of the behavioural interpretations or improving the overall fit of the models. This was found for the variety of movements of the species under observation in this study. The second part of this study attempts to validate the state allocation of the HMMs fitted to eland trajectories in the Greater Addo Elephant National Park in the Eastern Cape, with the use of camera trap data. This presented a unique opportunity as this type of data is mainly used for abundance or capture-recapture studies, and the HMMs are rarely validated as the true behaviours of the animals are seldom known. Results revealed that the same diel patterns were detected by the HMMs that were shown by the classified camera trap data. Direct comparisons of the observations where the dates and times matched for the telemetry and camera trap data could be done in several rare instances, which revealed many similarities. Although it was not an ideal comparison, the camera trap data provided a rough validation of the state allocation of the HMMs used in the study.
- Full Text:
- Date Issued: 2018
Predicting reintroduction outcomes: assessing the feasibility of reintroducing African wild dog to a small protected area
- Authors: Vogel, John Thomas
- Date: 2018
- Subjects: African wild dog , Wild dogs -- South Africa Wild dogs -- Conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/36464 , vital:33947
- Description: Large mammalian carnivores have experienced significant contractions in population sizes and geographical ranges. The conservation of large carnivores is crucially important, particularly due to their vulnerability to extinction and their functional significance and ability to structure ecosystems. Due to an expanding human footprint, large carnivores are increasingly subject to modified and spatially constrained habitats. A growing debate exists as to how to conserve and coexist with large carnivores in an anthropogenically induced environment. Reintroduction, as a conservation tool to restore locally extirpated large carnivores to portions of their former ranges is increasingly being applied. However, in South Africa, habitat to support large carnivores remain small and non-contiguous. Food is a fundamental ecological requirement to sustain reintroduced large carnivores. Therefore, an understanding of large predator foraging patterns can be informative in the context of how the predator species influences and utilises a novel ecosystem. We investigated the foraging behaviour of reintroduced African wild dog (Lycaon pictus) at five small protected areas in KwaZulu-Natal, South Africa. Wild dog utilised 16 prey species, albeit they primarily used nyala (Tragelaphus angasii) and impala (Aepyceros melampus) which collectively form 75 % of their diet. Only nyala was significantly preferred, suggesting that this prey species is used in greater proportion to their abundance. As wild dogs are social cooperative hunters, we tested whether wild dog pack size was correlated to prey mass selection. There was no evidence to suggest that larger packs use larger prey. However, the mean wild dog pack size in our sample sites, was relatively smaller than those encountered elsewhere. Furthermore, wild dog have been shown to modify their hunting behaviour in the presence of wildlife-proof fencing, by using fences to aid in the capture of larger prey species than would innately occur. We compared the prey mass of wild dog kills in relation to proximity of these hard boundaries. Despite the affinity towards kills occurring within 200 m of fences, the upward bias caused by fences on prey mass selection was inconsistent across sample sites. The relatively small size of wild dogs makes them particularly vulnerable to competition. As the energetic output of wild dog is high, interspecific competition can increase foraging costs. The reintroduction of large carnivores to small artificially induced systems may be a contentious issue as resources available to support large carnivores are expected to be relatively more finite. We compared both the potential inter- and intraspecific dietary niche dimensions of an intact large carnivore guild in context of a wild dog reintroduction. We determined cheetah (Acinonyx jubatus), leopard (Panthera pardus), and lion (Panthera leo) prey composition, diet breadth, overlap, prey preference and predicted the density of an intact large carnivore guild in a novel landscape. Further, we compared the foraging behaviour of these large carnivores to that of wild dog. Our findings suggest that large African carnivores in small protected areas are subject to a considerable dietary niche overlap. Wild dog and cheetah, particularly reproductive females with dependent offspring, displayed the greatest potential for dietary overlap and subsequent competition. Leopard and lion at the population species level exhibited greater degrees of foraging plasticity. Lion displayed a contrasting prey species preference to sympatric predators as they selected for prey items frequently avoided by cheetah, leopard and wild dog. The proposed wild dog reintroduction site is expected to sustain seven wild dog based on the availability of preferred prey biomass. However, the reintroduction of wild dog to the small protected area is expected to have negative lateral trophic influences on other species of conservation concern. This should be of vital importance to management of the protected area. As the influence of competition in food-web and population dynamics, particularly in resource poor environments may be profound, our research highlights the need to assess the influence of competitive forces in structuring and restoring large predators to portions of their historical range.
- Full Text:
- Date Issued: 2018
- Authors: Vogel, John Thomas
- Date: 2018
- Subjects: African wild dog , Wild dogs -- South Africa Wild dogs -- Conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/36464 , vital:33947
- Description: Large mammalian carnivores have experienced significant contractions in population sizes and geographical ranges. The conservation of large carnivores is crucially important, particularly due to their vulnerability to extinction and their functional significance and ability to structure ecosystems. Due to an expanding human footprint, large carnivores are increasingly subject to modified and spatially constrained habitats. A growing debate exists as to how to conserve and coexist with large carnivores in an anthropogenically induced environment. Reintroduction, as a conservation tool to restore locally extirpated large carnivores to portions of their former ranges is increasingly being applied. However, in South Africa, habitat to support large carnivores remain small and non-contiguous. Food is a fundamental ecological requirement to sustain reintroduced large carnivores. Therefore, an understanding of large predator foraging patterns can be informative in the context of how the predator species influences and utilises a novel ecosystem. We investigated the foraging behaviour of reintroduced African wild dog (Lycaon pictus) at five small protected areas in KwaZulu-Natal, South Africa. Wild dog utilised 16 prey species, albeit they primarily used nyala (Tragelaphus angasii) and impala (Aepyceros melampus) which collectively form 75 % of their diet. Only nyala was significantly preferred, suggesting that this prey species is used in greater proportion to their abundance. As wild dogs are social cooperative hunters, we tested whether wild dog pack size was correlated to prey mass selection. There was no evidence to suggest that larger packs use larger prey. However, the mean wild dog pack size in our sample sites, was relatively smaller than those encountered elsewhere. Furthermore, wild dog have been shown to modify their hunting behaviour in the presence of wildlife-proof fencing, by using fences to aid in the capture of larger prey species than would innately occur. We compared the prey mass of wild dog kills in relation to proximity of these hard boundaries. Despite the affinity towards kills occurring within 200 m of fences, the upward bias caused by fences on prey mass selection was inconsistent across sample sites. The relatively small size of wild dogs makes them particularly vulnerable to competition. As the energetic output of wild dog is high, interspecific competition can increase foraging costs. The reintroduction of large carnivores to small artificially induced systems may be a contentious issue as resources available to support large carnivores are expected to be relatively more finite. We compared both the potential inter- and intraspecific dietary niche dimensions of an intact large carnivore guild in context of a wild dog reintroduction. We determined cheetah (Acinonyx jubatus), leopard (Panthera pardus), and lion (Panthera leo) prey composition, diet breadth, overlap, prey preference and predicted the density of an intact large carnivore guild in a novel landscape. Further, we compared the foraging behaviour of these large carnivores to that of wild dog. Our findings suggest that large African carnivores in small protected areas are subject to a considerable dietary niche overlap. Wild dog and cheetah, particularly reproductive females with dependent offspring, displayed the greatest potential for dietary overlap and subsequent competition. Leopard and lion at the population species level exhibited greater degrees of foraging plasticity. Lion displayed a contrasting prey species preference to sympatric predators as they selected for prey items frequently avoided by cheetah, leopard and wild dog. The proposed wild dog reintroduction site is expected to sustain seven wild dog based on the availability of preferred prey biomass. However, the reintroduction of wild dog to the small protected area is expected to have negative lateral trophic influences on other species of conservation concern. This should be of vital importance to management of the protected area. As the influence of competition in food-web and population dynamics, particularly in resource poor environments may be profound, our research highlights the need to assess the influence of competitive forces in structuring and restoring large predators to portions of their historical range.
- Full Text:
- Date Issued: 2018
Investigation of the thermo-chemical behaviour of coal-algae agglomerates
- Authors: Baloyi, Hope
- Date: 2018
- Subjects: Biomass energy , Coal -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23913 , vital:30642
- Description: There is a growing research interest in the co-processing of biomass and coal, with the aim of addressing the negative attributes associated with the thermal processing of coal alone. Biomass feedstocks are regarded as a clean, renewable source, and the co-utilization of biomass feedstocks with coal is deemed to have a potential to reduce emission of pollutants (i.e. NOx and SOx) and volatile organic compounds (VOC’s). Moreover, biomass are thermally reactive and thus facilitate the conversion of coal during co-processing. Biomass material and coal are two autonomous fuel materials with different chemical characteristics and have a dissimilar thermal behaviour making it difficult to achieve chemical interaction between the two solid fuels to contribute to the formation of products. Coalgae® Technology developed at the Nelson Mandela University, involves the biological treatment of coal fines by adsorbing live microalgae biomass (in slurry form) onto waste coal fines to form coal-microalgae agglomerates. This new innovative approach seeks to integrate bio-based feedstock into coal thermal processing and to improve the utilization and thermal efficiency of coal fines as well as the interaction between the volatile components of biomass and coal during thermal processing (e.g. devolatilization), thereby overcoming some of the challenges that confront the co-processing of coal and biomass. Coal fines are low-ranked coals, generally characterized by high contents of sulphur, high ash yields, low calorific values and poor thermal reactivity, and these attributes limits the thermo-chemical processing of the coal fines. Therefore, this investigation was undertaken to assess the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorbing live microalgae slurry at varying ratios onto coal fines. For this purpose, the effects of adsorbing microalgae at varying ratios on the chemical characteristics and thermal behaviour of coal fines under pyrolytic conditions were investigated. The primary aim was to assess whether the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorption of live microalgae onto fine coal, is substantively modified compared to a simple additive model of the original coal and pre-dried microalgae biomass samples. Results obtained from the proximate analyses performed on an Eltra Thermo-gravimetric analyzer (TGA) thermostep, have shown that the adsorption of microalgae slurry onto coal fines does not possess greater influence in improving the yield of volatiles and ash in coal fines than can be expected from a simple additive model of the original raw materials. Based on the ultimate analyses results, it was found that the adsorption of microalgae slurry resulted in a systematic reduction in the sulphur content, a notable increase in the hydrogen and oxygen contents, however, no significant disparities were found between the measured ultimate properties of coal-microalgae agglomerates as compared to the theoretically-expected ultimate properties from a simple linear combination of parental coal and microalgae biomass. Assessment of the thermal behaviour of parental samples and coal-microalgae agglomerates involved the use non-isothermal (40-900ºC, 20 K/min) thermogravimetry under inert conditions. It was found that the adsorption of microalgae slurry onto coal fines resulted in an improved thermal reactivity of coal fines, although, did not affect the overall pyrolysis characteristics of the coal fines. Comparison of the thermal profiles (measured and calculated TG/DTG curves), revealed that the yield of volatile products during the pyrolysis of coal-microalgae blends do not exceed the expected volatile yields from a simple combination of coal and microalgae biomass. These results suggest that there was no positive or accelerative synergistic interaction between volatile components of adsorbed microalgae and coal fines during pyrolysis. Mild pyrolysis of raw coal and coal-microalgae performed in a fixed-bed reactor furnace (450ºC), resulted in improved yields of Fossil-Bio crude (FBC) oil (derived from coal-microalgae pyrolysis), at increased biomass ratio compared to coal tar. FBC Oil was found to contain relatively high contents of oxygen, hydrogen, and low sulphur content than coal tar. GC-MS analyses showed the presence of a heterocyclic compounds (i.e. Indole and 2, 6 dimethyl pyridine) in the FBC oil and these were not identified in the coal tar. Furthermore, high boiling compounds such as Flourene, pyrene and pentacosane were identified in the coal tar, however not identified in the FBC oil. Simulated distillation results showed notable differences between the FBC oil and coal tar in terms of the distribution of boiling point fractions particularly, high boing point components. Semi-devolatilized chars derived from coal-microalgae agglomerates showed substantial degree of decarboxylation and dehydrogenation compared to the coal chars.
- Full Text:
- Date Issued: 2018
- Authors: Baloyi, Hope
- Date: 2018
- Subjects: Biomass energy , Coal -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23913 , vital:30642
- Description: There is a growing research interest in the co-processing of biomass and coal, with the aim of addressing the negative attributes associated with the thermal processing of coal alone. Biomass feedstocks are regarded as a clean, renewable source, and the co-utilization of biomass feedstocks with coal is deemed to have a potential to reduce emission of pollutants (i.e. NOx and SOx) and volatile organic compounds (VOC’s). Moreover, biomass are thermally reactive and thus facilitate the conversion of coal during co-processing. Biomass material and coal are two autonomous fuel materials with different chemical characteristics and have a dissimilar thermal behaviour making it difficult to achieve chemical interaction between the two solid fuels to contribute to the formation of products. Coalgae® Technology developed at the Nelson Mandela University, involves the biological treatment of coal fines by adsorbing live microalgae biomass (in slurry form) onto waste coal fines to form coal-microalgae agglomerates. This new innovative approach seeks to integrate bio-based feedstock into coal thermal processing and to improve the utilization and thermal efficiency of coal fines as well as the interaction between the volatile components of biomass and coal during thermal processing (e.g. devolatilization), thereby overcoming some of the challenges that confront the co-processing of coal and biomass. Coal fines are low-ranked coals, generally characterized by high contents of sulphur, high ash yields, low calorific values and poor thermal reactivity, and these attributes limits the thermo-chemical processing of the coal fines. Therefore, this investigation was undertaken to assess the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorbing live microalgae slurry at varying ratios onto coal fines. For this purpose, the effects of adsorbing microalgae at varying ratios on the chemical characteristics and thermal behaviour of coal fines under pyrolytic conditions were investigated. The primary aim was to assess whether the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorption of live microalgae onto fine coal, is substantively modified compared to a simple additive model of the original coal and pre-dried microalgae biomass samples. Results obtained from the proximate analyses performed on an Eltra Thermo-gravimetric analyzer (TGA) thermostep, have shown that the adsorption of microalgae slurry onto coal fines does not possess greater influence in improving the yield of volatiles and ash in coal fines than can be expected from a simple additive model of the original raw materials. Based on the ultimate analyses results, it was found that the adsorption of microalgae slurry resulted in a systematic reduction in the sulphur content, a notable increase in the hydrogen and oxygen contents, however, no significant disparities were found between the measured ultimate properties of coal-microalgae agglomerates as compared to the theoretically-expected ultimate properties from a simple linear combination of parental coal and microalgae biomass. Assessment of the thermal behaviour of parental samples and coal-microalgae agglomerates involved the use non-isothermal (40-900ºC, 20 K/min) thermogravimetry under inert conditions. It was found that the adsorption of microalgae slurry onto coal fines resulted in an improved thermal reactivity of coal fines, although, did not affect the overall pyrolysis characteristics of the coal fines. Comparison of the thermal profiles (measured and calculated TG/DTG curves), revealed that the yield of volatile products during the pyrolysis of coal-microalgae blends do not exceed the expected volatile yields from a simple combination of coal and microalgae biomass. These results suggest that there was no positive or accelerative synergistic interaction between volatile components of adsorbed microalgae and coal fines during pyrolysis. Mild pyrolysis of raw coal and coal-microalgae performed in a fixed-bed reactor furnace (450ºC), resulted in improved yields of Fossil-Bio crude (FBC) oil (derived from coal-microalgae pyrolysis), at increased biomass ratio compared to coal tar. FBC Oil was found to contain relatively high contents of oxygen, hydrogen, and low sulphur content than coal tar. GC-MS analyses showed the presence of a heterocyclic compounds (i.e. Indole and 2, 6 dimethyl pyridine) in the FBC oil and these were not identified in the coal tar. Furthermore, high boiling compounds such as Flourene, pyrene and pentacosane were identified in the coal tar, however not identified in the FBC oil. Simulated distillation results showed notable differences between the FBC oil and coal tar in terms of the distribution of boiling point fractions particularly, high boing point components. Semi-devolatilized chars derived from coal-microalgae agglomerates showed substantial degree of decarboxylation and dehydrogenation compared to the coal chars.
- Full Text:
- Date Issued: 2018
The FAST fabric objective measurement properties of commercial worsted apparel fabrics available in South Africa
- Authors: Das, Sweta
- Date: 2018
- Subjects: Textile fabrics -- Testing , Textile fabrics -- Measurement , Textile fibers -- Testing , Textile design
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/17814 , vital:28458
- Description: In the last few decades, there has been a shift globally towards the objective measurement of these textile fibre, yarn and fabric properties which determine processing performance and product quality. This shift is also very apparent in the objective measurement of fabric properties, particularly those relating to handle and making-up into a garment. This study was motivated by the fact that the adoption of fabric objective measurement (FOM), specifically the FAST system, will benefit the South African worsted apparel sector, as it has done in various other countries which produce high quality worsted apparel fabrics and garments. FAST is robust and portable, yet inexpensive. The main objective of the study was to develop a FAST referencing system which can be used for benchmarking by the local apparel industry and, as a basis for encouraging and persuading the industry to adopt this system of fabric quality measurement and assurance and thereby improve their product quality and international competitiveness. To achieve the main objective, involved sourcing and FAST testing a representative cross-section of commercial worsted apparel fabrics with the emphasis on wool and wool blends from the local fabric and clothing manufacturing industry, and determining how the various FAST properties were affected by factors such as fabric weave, fibre blend and weight, since this could impact on the specific nature and validity of the referencing system. A total of some 394 worsted type commercial fabrics, mainly in wool and wool blends, were sourced from, and with the inputs of, local apparel fabric and clothing manufacturers so as to ensure the local fabric and garment representative of the sample population and after which the fabrics were tested on the FAST system. ANOVA (regression analysis) was carried out on each of the FAST parameters in order to determine whether fabric weight, weave, thickness and fibre composition (pure wool and wool blends) had a statistically significant effect on them, since this is an important aspect which needs to be clarified prior to the development of a envisaged meaningful FAST system.
- Full Text:
- Date Issued: 2018
- Authors: Das, Sweta
- Date: 2018
- Subjects: Textile fabrics -- Testing , Textile fabrics -- Measurement , Textile fibers -- Testing , Textile design
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/17814 , vital:28458
- Description: In the last few decades, there has been a shift globally towards the objective measurement of these textile fibre, yarn and fabric properties which determine processing performance and product quality. This shift is also very apparent in the objective measurement of fabric properties, particularly those relating to handle and making-up into a garment. This study was motivated by the fact that the adoption of fabric objective measurement (FOM), specifically the FAST system, will benefit the South African worsted apparel sector, as it has done in various other countries which produce high quality worsted apparel fabrics and garments. FAST is robust and portable, yet inexpensive. The main objective of the study was to develop a FAST referencing system which can be used for benchmarking by the local apparel industry and, as a basis for encouraging and persuading the industry to adopt this system of fabric quality measurement and assurance and thereby improve their product quality and international competitiveness. To achieve the main objective, involved sourcing and FAST testing a representative cross-section of commercial worsted apparel fabrics with the emphasis on wool and wool blends from the local fabric and clothing manufacturing industry, and determining how the various FAST properties were affected by factors such as fabric weave, fibre blend and weight, since this could impact on the specific nature and validity of the referencing system. A total of some 394 worsted type commercial fabrics, mainly in wool and wool blends, were sourced from, and with the inputs of, local apparel fabric and clothing manufacturers so as to ensure the local fabric and garment representative of the sample population and after which the fabrics were tested on the FAST system. ANOVA (regression analysis) was carried out on each of the FAST parameters in order to determine whether fabric weight, weave, thickness and fibre composition (pure wool and wool blends) had a statistically significant effect on them, since this is an important aspect which needs to be clarified prior to the development of a envisaged meaningful FAST system.
- Full Text:
- Date Issued: 2018
Assessment of indigenous forest degradation and deforestation along the wild coast, near Port St John’s, Eastern Cape Province, South Africa
- Katende, Lukyamuzi Lucky Fulgentius
- Authors: Katende, Lukyamuzi Lucky Fulgentius
- Date: 2018
- Subjects: Deforestation -- South Africa -- Eastern Cape , Forest degradation -- South Africa -- Eastern Cape Forest management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30649 , vital:31006
- Description: Indigenous forests along the Wild Coast of the Eastern Cape Province have experienced both degradation and deforestation over the past decades. In early 2000, steps were taken to rehabilitate some of the degraded areas. Nevertheless, there is no monitoring mechanism in place, so little is known about the extent of degradation and impact of the rehabilitation efforts. The present study assesses the extent to which deforestation and degradation of the indigenous forests have occurred, and evaluates rehabilitation efforts in the study area around Port Saint John’s. Forest degradation was defined as the decrease in forest cover density while deforestation was defined as an increase in the trend of light forests and/ or a decrease in dense forests. The details for this study were obtained from multi – temporal remotely sensed data for a period between 1982 and 2013 (31 years). Multi-temporal Landsat satellite imagery for 1982, 1986, 1989, 2002, 2009 and 2013 was acquired and analysed. On the basis of prior knowledge of the area, the supervised classification approach was used. The Maximum likelihood supervised classification technique was used to extract information from satellite data. The classified images were filtered using a majority filtering procedure to reduce noise. Google Earth (Astrium) ancillary images were used to refine the classification based on expert rules. The derived changes in the degraded and rehabilitated areas were further validated through field visits. The overall image classification accuracy generated from Landsat image data ranged from 80% to 90%. It was noted that the area of dense forest almost doubled between 1986 and 1989, coinciding with a 59% decrease in the light forest. Subsequently, dense forests increased by 14,820 ha while light forests decreased by 16,690 ha between 1989 and 2002. The subsequent reduction in light forest coverage is explained by the establishment of the Participatory Forest Management (PFMA) approach by Department of Water Affairs and Forestry (DWAF) which reversed the degradation trend. However, specific degradation hotspots were identified, particularly where new settlements have been established. The emergence of the non-vegetated area increased gradually from 7% in 1986 to 23.4% in 2013. Notably, dense forest was observed to have experienced higher rates of forest degradation and deforestation than the light forest. The highest number patches were 4 recorded between 2002 and 1998, followed by between 2010 and 2013 and lastly 1986. Based on spatial connectedness of patches, the year 1986 had the highest landscape connectedness of forest vegetation (CONAT = 35.3) followed by 2002 and 1996 while the year 2010 and 2013 had the lowest landscape contiguity. Over the study period, the distribution of patches clearly shows that forest degradation and deforestation rates were lower in the years 1986, 1998 and tremendously increased in the later period of between 2010 and 2013. However, as a result of rehabilitation efforts, dense forest was seen to steadily gain more land than light forest. Finer details of degradation trends could not be easily picked from the images used in the study, given their spatial resolution limitations. That notwithstanding, the trends identified are good for overview decisions. The study has also established that de-agraianisation, forest restoration and rehabilitation greatly contributed to increased forest cover. Therefore, with more use of GIS by forest managers, and imagery of the high resolution being readily available, forests will in future be easily monitored using remote sensing.
- Full Text:
- Date Issued: 2018
- Authors: Katende, Lukyamuzi Lucky Fulgentius
- Date: 2018
- Subjects: Deforestation -- South Africa -- Eastern Cape , Forest degradation -- South Africa -- Eastern Cape Forest management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30649 , vital:31006
- Description: Indigenous forests along the Wild Coast of the Eastern Cape Province have experienced both degradation and deforestation over the past decades. In early 2000, steps were taken to rehabilitate some of the degraded areas. Nevertheless, there is no monitoring mechanism in place, so little is known about the extent of degradation and impact of the rehabilitation efforts. The present study assesses the extent to which deforestation and degradation of the indigenous forests have occurred, and evaluates rehabilitation efforts in the study area around Port Saint John’s. Forest degradation was defined as the decrease in forest cover density while deforestation was defined as an increase in the trend of light forests and/ or a decrease in dense forests. The details for this study were obtained from multi – temporal remotely sensed data for a period between 1982 and 2013 (31 years). Multi-temporal Landsat satellite imagery for 1982, 1986, 1989, 2002, 2009 and 2013 was acquired and analysed. On the basis of prior knowledge of the area, the supervised classification approach was used. The Maximum likelihood supervised classification technique was used to extract information from satellite data. The classified images were filtered using a majority filtering procedure to reduce noise. Google Earth (Astrium) ancillary images were used to refine the classification based on expert rules. The derived changes in the degraded and rehabilitated areas were further validated through field visits. The overall image classification accuracy generated from Landsat image data ranged from 80% to 90%. It was noted that the area of dense forest almost doubled between 1986 and 1989, coinciding with a 59% decrease in the light forest. Subsequently, dense forests increased by 14,820 ha while light forests decreased by 16,690 ha between 1989 and 2002. The subsequent reduction in light forest coverage is explained by the establishment of the Participatory Forest Management (PFMA) approach by Department of Water Affairs and Forestry (DWAF) which reversed the degradation trend. However, specific degradation hotspots were identified, particularly where new settlements have been established. The emergence of the non-vegetated area increased gradually from 7% in 1986 to 23.4% in 2013. Notably, dense forest was observed to have experienced higher rates of forest degradation and deforestation than the light forest. The highest number patches were 4 recorded between 2002 and 1998, followed by between 2010 and 2013 and lastly 1986. Based on spatial connectedness of patches, the year 1986 had the highest landscape connectedness of forest vegetation (CONAT = 35.3) followed by 2002 and 1996 while the year 2010 and 2013 had the lowest landscape contiguity. Over the study period, the distribution of patches clearly shows that forest degradation and deforestation rates were lower in the years 1986, 1998 and tremendously increased in the later period of between 2010 and 2013. However, as a result of rehabilitation efforts, dense forest was seen to steadily gain more land than light forest. Finer details of degradation trends could not be easily picked from the images used in the study, given their spatial resolution limitations. That notwithstanding, the trends identified are good for overview decisions. The study has also established that de-agraianisation, forest restoration and rehabilitation greatly contributed to increased forest cover. Therefore, with more use of GIS by forest managers, and imagery of the high resolution being readily available, forests will in future be easily monitored using remote sensing.
- Full Text:
- Date Issued: 2018
Assessing growth response patterns of microalgae to varying environmental conditions using sediments from ephemeral wetlands
- Authors: Mazwane, Sixolile
- Date: 2018
- Subjects: Microalgae -- South Africa , Wetland conservation -- South Africa Landscape ecology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/22398 , vital:29966
- Description: Ephemeral wetlands serve as habitats for different fauna and flora. Microalgae make up a bulk of primary production in newly inundated wetlands and have a big influence on the aquatic community structure. As ephemeral wetlands have cyclical drying and wetting phases, algal species found in these habitats have evolved adaptations (i.e. dormancy and cysts) to survive during dry periods and become productive upon inundation. Algae have been used as ecological indicators in many aquatic ecosystems including wetlands, particularly associated with water quality and with varying inundation states. Whilst temperature and water level have been shown to affect microalgal biomass independently, this study aims to assess the effects of these two factors simultaneously on microalgal biomass, species diversity and community changes. The aim of the study was to investigate microalgal dynamics using two ephemeral wetlands in the Nelson Mandela Bay Municipality. The growth response patterns of microalgal communities to varying temperatures and inundation levels through experiments using dry temporary wetland sediments was also assessed. Dry sediments were collected from two depression wetlands with different sediment characteristics (1592 and Elephant Wallow (EW)). The study consisted of replicated treatments with three different surface area: volume ratios (SA:V) (Low surface area: volume (LSAV), Medium (MSAV) and High (HSAV)), inundated with distilled water. The microcosms were placed inside a Conviron environmental growth chamber and illuminated at between 106 to 138 μmol.m-2s-1, with a 12:12 light-dark cycle at constant temperatures of 12 °C (mean winter) and 22 °C (mean summer). Physicochemical data were recorded, and biological samples were collected every 4 days over a 28-day inundation period. In the field, Electrical conductivity (EC) and pH showed a gradual increase over the inundation period. The systems were well oxygenated (6-10 mg L-1). Overall, phytoplankton biomass in the field was ~10 μg L-1 whereas benthic biomass (MPB) was significantly higher 10 – 300 mg L-1. Within the experiments, the phytoplankton biomass showed a pattern while MPB biomass increased gradually for both experiments. There were no significant (p>0.05) differences between the 1592 and EW phytoplankton biomass. However, the MPB biomass showed significant (p<0.05) differences between the two sediments (EW high, 1592 low) and the experiments, respectively. In the winter experiment, the 1592 sediment had high phytoplankton biomass compared to EW, the reverse was observed for the MPB biomass. In summer experiment, similar results were observed in terms of phytoplankton biomass. For the MPBs, 1592 had greater biomass for all treatments compared to EW. The phytoplankton community for both field samples and experiments was dominated by chlorophytes (>50 %), followed by cyanophytes and euglenophytes (with cryptophytes in summer experiment) for both field and experiments. The MPB community was dominated by bacillariophytes (55 %) and chlorophytes (25 %) in the 1592 sediments and this was also the case with EW sediments bacillariophytes (70 %), although cyanophytes (15 %) made up the remainder of the MPB community. The 1592 sediments supported higher diatom abundances under the HSAV compared to the same treatment from the EW wetland suggesting that possibly grain size or texture might be responsible. In contrast, sediments from EW under LSAV conditions showed higher diatom cell numbers possibly indicating that increased water depth has the propensity to support greater diatom assemblages compared with the same water level for sediments from 1592. The results indicate that microalgae community is influenced by sediment type more than it is by water level. From this study it was also established that microalgae were able to reemerge from their dormancy state upon inundation and that tank experiments were successful in simulating natural ephemeral wetland conditions. The findings from this study will add to the available baseline data on microalgal dynamics in the ephemeral wetlands in South Africa, especially in semi-arid areas. This information can be valuable in enhancing our understanding regarding the role of microalgae in ephemeral wetlands in order to conserve them.
- Full Text:
- Date Issued: 2018
- Authors: Mazwane, Sixolile
- Date: 2018
- Subjects: Microalgae -- South Africa , Wetland conservation -- South Africa Landscape ecology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/22398 , vital:29966
- Description: Ephemeral wetlands serve as habitats for different fauna and flora. Microalgae make up a bulk of primary production in newly inundated wetlands and have a big influence on the aquatic community structure. As ephemeral wetlands have cyclical drying and wetting phases, algal species found in these habitats have evolved adaptations (i.e. dormancy and cysts) to survive during dry periods and become productive upon inundation. Algae have been used as ecological indicators in many aquatic ecosystems including wetlands, particularly associated with water quality and with varying inundation states. Whilst temperature and water level have been shown to affect microalgal biomass independently, this study aims to assess the effects of these two factors simultaneously on microalgal biomass, species diversity and community changes. The aim of the study was to investigate microalgal dynamics using two ephemeral wetlands in the Nelson Mandela Bay Municipality. The growth response patterns of microalgal communities to varying temperatures and inundation levels through experiments using dry temporary wetland sediments was also assessed. Dry sediments were collected from two depression wetlands with different sediment characteristics (1592 and Elephant Wallow (EW)). The study consisted of replicated treatments with three different surface area: volume ratios (SA:V) (Low surface area: volume (LSAV), Medium (MSAV) and High (HSAV)), inundated with distilled water. The microcosms were placed inside a Conviron environmental growth chamber and illuminated at between 106 to 138 μmol.m-2s-1, with a 12:12 light-dark cycle at constant temperatures of 12 °C (mean winter) and 22 °C (mean summer). Physicochemical data were recorded, and biological samples were collected every 4 days over a 28-day inundation period. In the field, Electrical conductivity (EC) and pH showed a gradual increase over the inundation period. The systems were well oxygenated (6-10 mg L-1). Overall, phytoplankton biomass in the field was ~10 μg L-1 whereas benthic biomass (MPB) was significantly higher 10 – 300 mg L-1. Within the experiments, the phytoplankton biomass showed a pattern while MPB biomass increased gradually for both experiments. There were no significant (p>0.05) differences between the 1592 and EW phytoplankton biomass. However, the MPB biomass showed significant (p<0.05) differences between the two sediments (EW high, 1592 low) and the experiments, respectively. In the winter experiment, the 1592 sediment had high phytoplankton biomass compared to EW, the reverse was observed for the MPB biomass. In summer experiment, similar results were observed in terms of phytoplankton biomass. For the MPBs, 1592 had greater biomass for all treatments compared to EW. The phytoplankton community for both field samples and experiments was dominated by chlorophytes (>50 %), followed by cyanophytes and euglenophytes (with cryptophytes in summer experiment) for both field and experiments. The MPB community was dominated by bacillariophytes (55 %) and chlorophytes (25 %) in the 1592 sediments and this was also the case with EW sediments bacillariophytes (70 %), although cyanophytes (15 %) made up the remainder of the MPB community. The 1592 sediments supported higher diatom abundances under the HSAV compared to the same treatment from the EW wetland suggesting that possibly grain size or texture might be responsible. In contrast, sediments from EW under LSAV conditions showed higher diatom cell numbers possibly indicating that increased water depth has the propensity to support greater diatom assemblages compared with the same water level for sediments from 1592. The results indicate that microalgae community is influenced by sediment type more than it is by water level. From this study it was also established that microalgae were able to reemerge from their dormancy state upon inundation and that tank experiments were successful in simulating natural ephemeral wetland conditions. The findings from this study will add to the available baseline data on microalgal dynamics in the ephemeral wetlands in South Africa, especially in semi-arid areas. This information can be valuable in enhancing our understanding regarding the role of microalgae in ephemeral wetlands in order to conserve them.
- Full Text:
- Date Issued: 2018
Incidence of bacterial infections and colonisation in patients admitted to a tuberculosis hospital
- Authors: Annear, Dale John
- Date: 2018
- Subjects: Medical microbiology , Microbiology Bacteriology Tuberculosis -- Hospitals -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/21482 , vital:29526
- Description: Patients with drug resistant tuberculosis (TB) are treated with multiple antibiotics including moxifloxacin, linezolid, and meropenem, which puts them at greater risk for colonisation by multi-drug resistant (MDR) bacteria. The objectives of this study were to: (i) assess the antimicrobial prescribing patterns practiced within the hospital by retrospective patient file review; (ii) determine the spectrum of bacterial colonisation in TB patients upon admission and during hospitalisation; (iii) identify bacterial isolates and evaluate antimicrobial susceptibility profiles; (iv) detect antimicrobial resistance genes in the bacterial isolates by PCR and DNA sequencing; and (v) investigate genetic relatedness of Klebsiella pneumoniae isolates using Multi Locus Sequence Typing. Nasal, groin and rectal swabs [for the detection of extended spectrum beta lactamases (EBSLs), carbapenem-resistant Enterobacteriaceae (CRE), vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA)] were analysed from a cohort of patients (n=37) admitted either from the community (n = 28) or from other healthcare facilities (n=9) to a TB hospital. Swab samples were collected at admission and at four week intervals thereafter during hospitalization. Identification and antimicrobial susceptibility testing of bacterial isolates (n=62) were determined at the National Health Laboratory Services (NHLS) by the VITEK-MS and Vitek 2 systems respectively. Additional antimicrobial susceptibility testing was conducted by Sensititre Gram Negative Xtra (GNFX2) MIC plates. PCR and DNA sequencing were used for detection of resistance genes. Patients (n=13/37; 35%) were colonized by MDR bacteria (ESBLs [n=11], MRSA [n=2]) on admission. Colonization rates were lower in patients admitted from the community (9/28; 32%) compared to those transferred from other healthcare facilities (4/9; 44%). All admitted patients who did not exhibit colonization at baseline and who were resident within the hospital for longer than 4 weeks (17/37; 46% of total patients) became colonised by an ESBL-producing Enterobacteriaceae species. No patients acquired MRSA during hospitalisation. Among ESBL Enterobacteriaceae, Escherichia coli (41/62; 66%) and K. pneumoniae [14/62; 23%]) predominated. Nineteen percent (7/37) of patients demised during their hospitalization. Both the Vitek system and Sensititre Gram Negative Xtra (GNFX2) MIC plates susceptibilities were similar for most antimicrobials, however there were discrepancies for tigecycline susceptibility profiles. A high number of isolates exhibited resistance to aminoglycosides and fluoroquinolones. Genes encoding for ESBLs (CTX-M-14, CTX-M-15, SHV-28, OXA-1, and OXY-2-9) were detected among ESBL Enterobacteriaceae. Two Enterobacteriaceae isolates with reduced carbapenem susceptibility did not contain carbapenemase-encoding genes. MLST revealed unique sequence types and genetic diversity among the K. pneumoniae isolates from hospitalised patients. However, the source and colonization routes of these isolates could not be determined, which requires further investigation. This study provides insight into the spectrum of bacterial pathogen colonisation in hospitalised TB patients and suggests a review of infection control programs and practices at the TB hospital.
- Full Text:
- Date Issued: 2018
- Authors: Annear, Dale John
- Date: 2018
- Subjects: Medical microbiology , Microbiology Bacteriology Tuberculosis -- Hospitals -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/21482 , vital:29526
- Description: Patients with drug resistant tuberculosis (TB) are treated with multiple antibiotics including moxifloxacin, linezolid, and meropenem, which puts them at greater risk for colonisation by multi-drug resistant (MDR) bacteria. The objectives of this study were to: (i) assess the antimicrobial prescribing patterns practiced within the hospital by retrospective patient file review; (ii) determine the spectrum of bacterial colonisation in TB patients upon admission and during hospitalisation; (iii) identify bacterial isolates and evaluate antimicrobial susceptibility profiles; (iv) detect antimicrobial resistance genes in the bacterial isolates by PCR and DNA sequencing; and (v) investigate genetic relatedness of Klebsiella pneumoniae isolates using Multi Locus Sequence Typing. Nasal, groin and rectal swabs [for the detection of extended spectrum beta lactamases (EBSLs), carbapenem-resistant Enterobacteriaceae (CRE), vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA)] were analysed from a cohort of patients (n=37) admitted either from the community (n = 28) or from other healthcare facilities (n=9) to a TB hospital. Swab samples were collected at admission and at four week intervals thereafter during hospitalization. Identification and antimicrobial susceptibility testing of bacterial isolates (n=62) were determined at the National Health Laboratory Services (NHLS) by the VITEK-MS and Vitek 2 systems respectively. Additional antimicrobial susceptibility testing was conducted by Sensititre Gram Negative Xtra (GNFX2) MIC plates. PCR and DNA sequencing were used for detection of resistance genes. Patients (n=13/37; 35%) were colonized by MDR bacteria (ESBLs [n=11], MRSA [n=2]) on admission. Colonization rates were lower in patients admitted from the community (9/28; 32%) compared to those transferred from other healthcare facilities (4/9; 44%). All admitted patients who did not exhibit colonization at baseline and who were resident within the hospital for longer than 4 weeks (17/37; 46% of total patients) became colonised by an ESBL-producing Enterobacteriaceae species. No patients acquired MRSA during hospitalisation. Among ESBL Enterobacteriaceae, Escherichia coli (41/62; 66%) and K. pneumoniae [14/62; 23%]) predominated. Nineteen percent (7/37) of patients demised during their hospitalization. Both the Vitek system and Sensititre Gram Negative Xtra (GNFX2) MIC plates susceptibilities were similar for most antimicrobials, however there were discrepancies for tigecycline susceptibility profiles. A high number of isolates exhibited resistance to aminoglycosides and fluoroquinolones. Genes encoding for ESBLs (CTX-M-14, CTX-M-15, SHV-28, OXA-1, and OXY-2-9) were detected among ESBL Enterobacteriaceae. Two Enterobacteriaceae isolates with reduced carbapenem susceptibility did not contain carbapenemase-encoding genes. MLST revealed unique sequence types and genetic diversity among the K. pneumoniae isolates from hospitalised patients. However, the source and colonization routes of these isolates could not be determined, which requires further investigation. This study provides insight into the spectrum of bacterial pathogen colonisation in hospitalised TB patients and suggests a review of infection control programs and practices at the TB hospital.
- Full Text:
- Date Issued: 2018
Passive acoustic monitoring of coastal dolphins (Sousa plumbea and Tursiops aduncus) in Plettenberg Bay: temporal patterns and group dynamics
- Authors: Hlati, Kuhle
- Date: 2018
- Subjects: Marine mammals -- Effect of noise on -- Research , Oceanography Aquatic ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30050 , vital:30813
- Description: In this study, passive acoustic monitoring (PAM) was used to investigate the diel patterns of occurrence and activities of coastal dolphins (Sousa plumbea and Tursiops aduncus) in Plettenberg Bay, during April 2015 - July 2017. Automatic detectors were used to extract dolphin whistles and echolocation clicks from the acoustic recordings. The vocalizations were extracted without identifying the dolphin species due to insufficient visual observation data collected concurrent with the acoustic recordings to conclusively discriminate the two species. The correct detection performance and false alarm rate of the detectors for each acoustic recorder were evaluated and automatic detection protocols were developed. For a recorder that was initially deployed in the study (DSG recorder), the detectors achieved 64 - 80% true detection rates with false alarm rates of 10 - 22%. A more modern recorder (MicroMARS recorder) which replaced this one had a lower signal-to-noise ratio (SNR) and could sample at a higher frequency, the detectors achieved greater than 80% true detection rates and less than 5% false alarm rates. It was evident that SNR and sampling rate influenced the detection performance of the detectors. When acoustic recordings were compared to visual observations which were conducted concurrently with some of the recordings, it was apparent that acoustic recorders performed nearly twice as well as land-based observers in terms of overall detection of dolphin groups. Group size and behaviour affected acoustic detection rates of dolphins; large groups that were visually detected and which were foraging at the time, were more likely to be acoustically detected than small groups or single animals or groups associated travelling at the time. The detection rates of echolocation clicks and whistles showed a diel pattern of occurrence of coastal dolphins in Plettenberg Bay with a higher nighttime than daytime occurrence. The detection rates were higher from midday to midnight with a peak during dusk. The high activity of dolphins in the late afternoon and evening reported in this study was also supported by visual data (afternoons) in the area and acoustic studies in other regions. Comparison of acoustic detection rates and visual data from this study with past observations suggest that the occurrence patterns of T. aduncus in the bay appear to have changed; such a change may be influenced by changes in prey behaviour or distribution, or other factors. The significally higher detection rates of echolocation clicks than whistles and dominant foraging behaviour based on visual data suggest that Robberg may be an important feeding ground for the coastal dolphins that are frequenting Plettenberg Bay. The current study builds on a growing body of literature demonstrating that PAM and automatic detectors are powerful and effective tools for monitoring cetacean species. However, there are several factors that limit the detection of vocalization such as direction of the sound source, distance from hydrophone, sound propagation conditions, low SNR and acoustic masking. Regardless of these limitations, results from PAM showed diel patterns of occurrence of the coastal dolphins in the bay which could have not been achieved from visual monitoring, in particular the high nighttime occurrence patterns. PAM holds potential to be largely utilized in South Africa for dolphin and other cetacean studies. Further research and monitoring using PAM could potentially assist to better understand the drivers of the dolphin occurrence and activity patterns in the bay.
- Full Text:
- Date Issued: 2018
- Authors: Hlati, Kuhle
- Date: 2018
- Subjects: Marine mammals -- Effect of noise on -- Research , Oceanography Aquatic ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30050 , vital:30813
- Description: In this study, passive acoustic monitoring (PAM) was used to investigate the diel patterns of occurrence and activities of coastal dolphins (Sousa plumbea and Tursiops aduncus) in Plettenberg Bay, during April 2015 - July 2017. Automatic detectors were used to extract dolphin whistles and echolocation clicks from the acoustic recordings. The vocalizations were extracted without identifying the dolphin species due to insufficient visual observation data collected concurrent with the acoustic recordings to conclusively discriminate the two species. The correct detection performance and false alarm rate of the detectors for each acoustic recorder were evaluated and automatic detection protocols were developed. For a recorder that was initially deployed in the study (DSG recorder), the detectors achieved 64 - 80% true detection rates with false alarm rates of 10 - 22%. A more modern recorder (MicroMARS recorder) which replaced this one had a lower signal-to-noise ratio (SNR) and could sample at a higher frequency, the detectors achieved greater than 80% true detection rates and less than 5% false alarm rates. It was evident that SNR and sampling rate influenced the detection performance of the detectors. When acoustic recordings were compared to visual observations which were conducted concurrently with some of the recordings, it was apparent that acoustic recorders performed nearly twice as well as land-based observers in terms of overall detection of dolphin groups. Group size and behaviour affected acoustic detection rates of dolphins; large groups that were visually detected and which were foraging at the time, were more likely to be acoustically detected than small groups or single animals or groups associated travelling at the time. The detection rates of echolocation clicks and whistles showed a diel pattern of occurrence of coastal dolphins in Plettenberg Bay with a higher nighttime than daytime occurrence. The detection rates were higher from midday to midnight with a peak during dusk. The high activity of dolphins in the late afternoon and evening reported in this study was also supported by visual data (afternoons) in the area and acoustic studies in other regions. Comparison of acoustic detection rates and visual data from this study with past observations suggest that the occurrence patterns of T. aduncus in the bay appear to have changed; such a change may be influenced by changes in prey behaviour or distribution, or other factors. The significally higher detection rates of echolocation clicks than whistles and dominant foraging behaviour based on visual data suggest that Robberg may be an important feeding ground for the coastal dolphins that are frequenting Plettenberg Bay. The current study builds on a growing body of literature demonstrating that PAM and automatic detectors are powerful and effective tools for monitoring cetacean species. However, there are several factors that limit the detection of vocalization such as direction of the sound source, distance from hydrophone, sound propagation conditions, low SNR and acoustic masking. Regardless of these limitations, results from PAM showed diel patterns of occurrence of the coastal dolphins in the bay which could have not been achieved from visual monitoring, in particular the high nighttime occurrence patterns. PAM holds potential to be largely utilized in South Africa for dolphin and other cetacean studies. Further research and monitoring using PAM could potentially assist to better understand the drivers of the dolphin occurrence and activity patterns in the bay.
- Full Text:
- Date Issued: 2018
Testing the application of coastal altimetry in two South-Eastern African bights: the relationship between mesoscale features and chlorophyll-a, cyclonic eddies in the Indian Ocean
- Authors: Ziegler, Lisa
- Date: 2018
- Subjects: Oceanography -- Remote sensing -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/15225 , vital:28188
- Description: Enhanced coastal altimetry, adaptive Leading-Edge Subwaveform(ALES), was used to study wo well known eddies along the southeastern African coast, namely the Delagoa Bight Lee Eddy and the Durban Lee Eddy. I address a two part problem. Firstly, how well does the application of coastal altimetry techniques behave in the coastal region? Secondly, is there a relationship between the eddies’ presence and chlorophyll-a (chl-a). Sea level anomalies (SLA) were computed from the re-tracked ALES data of two satellite missions — Envisat (used for Delagoa Bight eddy) and Jason -2 (for the Durban eddy) with geophysical corrections removed. These datasets were compared with the original satellite, 1 Hz RADS, AND AVISO gridded data. Two regions were selected to see if there was a biophysical link. A centre region of the eddy and an outside region of the eddy were taken. Results indicate that coastal altimetrywas successful in delimiting both features. ALES was less noisey and able to recover more data that were missed by the 1 Hz RADS dataset. Hovmöller plots showed the Delagoa Bight eddy to be more of a transient feature than semi-permanent, as had previously been suggested. Results from the linear model indicate a negative correlation between SLA and chl-a. This influence could be through facilitation of chl-a in the bight or just retaining chla. This is hard to elucidate without in situ data. These results show a promising indication that coastal altimetry will be a useful and reliable product to further biophysical coupling research along the coast.
- Full Text:
- Date Issued: 2018
- Authors: Ziegler, Lisa
- Date: 2018
- Subjects: Oceanography -- Remote sensing -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/15225 , vital:28188
- Description: Enhanced coastal altimetry, adaptive Leading-Edge Subwaveform(ALES), was used to study wo well known eddies along the southeastern African coast, namely the Delagoa Bight Lee Eddy and the Durban Lee Eddy. I address a two part problem. Firstly, how well does the application of coastal altimetry techniques behave in the coastal region? Secondly, is there a relationship between the eddies’ presence and chlorophyll-a (chl-a). Sea level anomalies (SLA) were computed from the re-tracked ALES data of two satellite missions — Envisat (used for Delagoa Bight eddy) and Jason -2 (for the Durban eddy) with geophysical corrections removed. These datasets were compared with the original satellite, 1 Hz RADS, AND AVISO gridded data. Two regions were selected to see if there was a biophysical link. A centre region of the eddy and an outside region of the eddy were taken. Results indicate that coastal altimetrywas successful in delimiting both features. ALES was less noisey and able to recover more data that were missed by the 1 Hz RADS dataset. Hovmöller plots showed the Delagoa Bight eddy to be more of a transient feature than semi-permanent, as had previously been suggested. Results from the linear model indicate a negative correlation between SLA and chl-a. This influence could be through facilitation of chl-a in the bight or just retaining chla. This is hard to elucidate without in situ data. These results show a promising indication that coastal altimetry will be a useful and reliable product to further biophysical coupling research along the coast.
- Full Text:
- Date Issued: 2018
Environmental management of wetlands on an urban periphery: the case of Bay West
- Authors: Domoney, Nicola Leah
- Date: 2018
- Subjects: Wetland management -- South Africa -- Nelson Mandela Bay Municipality , Remote sensing Chlorophyll
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30854 , vital:31183
- Description: South Africa is a semi-arid country and thus depends on sound management of its water resources. Wetlands, despite their importance to water resource storage and water quality, among their other ecological and social significance, remain the most threatened of South Africa’s ecosystems. One of their greatest threats is encroachment by urbanisation. This occurs because wetlands, and especially ephemeral urban periphery wetlands, are overlooked. This study’s main objective was to determine the water quality and health of the wetlands adjacent to the newly established Bay West development on the western urban periphery of Nelson Mandela Bay. Wetlands are difficult to access and it is time-consuming to assess them. Remote sensing, however, overcomes this challenge and is cost-effective and time-considerate. It was the aim of this study to assess the health of the peri-urban wetlands on the Bay West periphery in order to formulate an appropriate environmental management plan, and remote sensing was tested as an effective method for wetland health management. It was found that the wetlands closer to the urban residential area (Sherwood suburb) were far more impacted than the wetlands nearer to Bay West Mall. Wetlands Six to Eight were higher in all parameters of pH, total dissolved solutes (TDS), salinity, conductivity and chlorophyll a concentration than Wetlands One to Five located further away from the urban area. A correlation with distance away from the urban area found a strong inverse relationship with between distance and the water quality factor, yielding a correlation coefficient of (<-0.7) in 2017 and (<-0.9) in 2018 with regard to TDS, salinity and conductivity. This, therefore, suggests that the wetlands’ health deteriorates towards the urban area. TDS, salinity, pH and conductivity correlate highly with chlorophyll a concentration at (>0.70). There was significant difference in pH in the year 2017 between the Bay West wetlands and the reference wetland (p<0.05; df=19; f= 3.56; n=3) and in 2018 (p<0.01; df=22; f= 8.90; n=3). No significant difference was found between the wetlands for dissolved oxygen. TDS between the wetlands was found to be significantly different in the year 2017 (p<0.01; df=23; f= 44.16; n=3) and 2018 (p<0.05; df=22; f=2.62; n=3), where conductivity and salinity returned the same p value for 2017 and 2018 as TDS respectively. There was a significant difference in chlorophyll a between the Bay West wetlands in 2017 (p<0.05; df=20; f=3.29; n=3) and Wetland Seven measured eutrophic at 22.45 mg/L-1. Remote sensing red band values and the field sample absorption values were highly correlated at R2= 0.5. Chlorophyll a content of wetland water correlated with NDVI data at R2= 0.6. Thus, remote sensing proved to be a successful estimator for wetlands health analysis and a proxy for wetland management. These results yielded an environmental management plan where monitoring water quality via chlorophyll a indicated health levels of the wetlands via trophic state.
- Full Text:
- Date Issued: 2018
- Authors: Domoney, Nicola Leah
- Date: 2018
- Subjects: Wetland management -- South Africa -- Nelson Mandela Bay Municipality , Remote sensing Chlorophyll
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30854 , vital:31183
- Description: South Africa is a semi-arid country and thus depends on sound management of its water resources. Wetlands, despite their importance to water resource storage and water quality, among their other ecological and social significance, remain the most threatened of South Africa’s ecosystems. One of their greatest threats is encroachment by urbanisation. This occurs because wetlands, and especially ephemeral urban periphery wetlands, are overlooked. This study’s main objective was to determine the water quality and health of the wetlands adjacent to the newly established Bay West development on the western urban periphery of Nelson Mandela Bay. Wetlands are difficult to access and it is time-consuming to assess them. Remote sensing, however, overcomes this challenge and is cost-effective and time-considerate. It was the aim of this study to assess the health of the peri-urban wetlands on the Bay West periphery in order to formulate an appropriate environmental management plan, and remote sensing was tested as an effective method for wetland health management. It was found that the wetlands closer to the urban residential area (Sherwood suburb) were far more impacted than the wetlands nearer to Bay West Mall. Wetlands Six to Eight were higher in all parameters of pH, total dissolved solutes (TDS), salinity, conductivity and chlorophyll a concentration than Wetlands One to Five located further away from the urban area. A correlation with distance away from the urban area found a strong inverse relationship with between distance and the water quality factor, yielding a correlation coefficient of (<-0.7) in 2017 and (<-0.9) in 2018 with regard to TDS, salinity and conductivity. This, therefore, suggests that the wetlands’ health deteriorates towards the urban area. TDS, salinity, pH and conductivity correlate highly with chlorophyll a concentration at (>0.70). There was significant difference in pH in the year 2017 between the Bay West wetlands and the reference wetland (p<0.05; df=19; f= 3.56; n=3) and in 2018 (p<0.01; df=22; f= 8.90; n=3). No significant difference was found between the wetlands for dissolved oxygen. TDS between the wetlands was found to be significantly different in the year 2017 (p<0.01; df=23; f= 44.16; n=3) and 2018 (p<0.05; df=22; f=2.62; n=3), where conductivity and salinity returned the same p value for 2017 and 2018 as TDS respectively. There was a significant difference in chlorophyll a between the Bay West wetlands in 2017 (p<0.05; df=20; f=3.29; n=3) and Wetland Seven measured eutrophic at 22.45 mg/L-1. Remote sensing red band values and the field sample absorption values were highly correlated at R2= 0.5. Chlorophyll a content of wetland water correlated with NDVI data at R2= 0.6. Thus, remote sensing proved to be a successful estimator for wetlands health analysis and a proxy for wetland management. These results yielded an environmental management plan where monitoring water quality via chlorophyll a indicated health levels of the wetlands via trophic state.
- Full Text:
- Date Issued: 2018
Vanadium-based catalysts for oxidation of organosulfur compounds: synthesis, catalysis and mechanistic studies
- Dembaremba, Tendai, Ogunlaja, Adeniyi
- Authors: Dembaremba, Tendai , Ogunlaja, Adeniyi
- Date: 2018
- Subjects: Organosulfur compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30248 , vital:30909
- Description: A series of oxidovanadium(IV) complexes based on the ligand, 2-(2’-hydroxyphenyl)imidazole, with substituent groups of different electronegativities on the phenolic para position, were successfully synthesized, characterized and investigated for their catalytic activities in the oxidation of dibenzothiophene (DBT), a typical refractory sulfur compound found in fuel. It was observed from catalytic oxidation studies that the presence of an electron withdrawing group on the phenolic para position of the ligand results in higher catalytic activity. SC-XRD data and DFT studies were used to explain the trends in activity observed. The highest activity was observed with 6.5 nmol of the nitro derivative catalyst [VO(PIMNO2)2] when 100% of 100 mg (0.543 mmol) of DBT was converted to its sulfone derivative dibenzothiophene sulfoxide (DBTO2) using 2.0 mL (1.05 mmol) of t-BuOOH. Potential to immobilize the complex catalysts was demonstrated through the synthesis of oxidovanadium(IV) copolymer nanofibers. The oxidovanadium nanofibers were successfully employed in the oxidation of sulfur compounds in a real fuel sample (diesel 500) which were then removed through solvent extraction using acetonitrile to give clean fuel. SC-XRD, EPR and UV-Vis spectroscopy were instrumental in providing insight into the mechanism of the catalyzed reaction. Vanadium oxides were also investigated as a cheaper alternative for the catalytic oxidation reaction. Phases of different vanadium oxides were synthesized by calcining NH4VO3 in air at different temperatures with an intention to investigate them for their catalytic activities. The catalyst obtained from calcination at 600⁰C was predominantly the orthorhombic phase of V2O5. Potential to immobilize the vanadium oxides was demonstrated using a silica support where NH4VO3 was impregnated onto silica and calcined in air at 600⁰C. The catalyst showed good potential in the oxidation of DBT to DBTO2, with 10 mg (43.9 μmol) of catalyst successfully converting 100% of 100 mg (0.543 mmol) DBT to DBTO2 using 2.0 mL (1.05 mmol) of t-BuOOH. The catalyst was also employed for a real fuel sample (diesel 500) with good results. The mechanistic aspects of vanadium oxides were also investigated in this study.
- Full Text:
- Date Issued: 2018
- Authors: Dembaremba, Tendai , Ogunlaja, Adeniyi
- Date: 2018
- Subjects: Organosulfur compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30248 , vital:30909
- Description: A series of oxidovanadium(IV) complexes based on the ligand, 2-(2’-hydroxyphenyl)imidazole, with substituent groups of different electronegativities on the phenolic para position, were successfully synthesized, characterized and investigated for their catalytic activities in the oxidation of dibenzothiophene (DBT), a typical refractory sulfur compound found in fuel. It was observed from catalytic oxidation studies that the presence of an electron withdrawing group on the phenolic para position of the ligand results in higher catalytic activity. SC-XRD data and DFT studies were used to explain the trends in activity observed. The highest activity was observed with 6.5 nmol of the nitro derivative catalyst [VO(PIMNO2)2] when 100% of 100 mg (0.543 mmol) of DBT was converted to its sulfone derivative dibenzothiophene sulfoxide (DBTO2) using 2.0 mL (1.05 mmol) of t-BuOOH. Potential to immobilize the complex catalysts was demonstrated through the synthesis of oxidovanadium(IV) copolymer nanofibers. The oxidovanadium nanofibers were successfully employed in the oxidation of sulfur compounds in a real fuel sample (diesel 500) which were then removed through solvent extraction using acetonitrile to give clean fuel. SC-XRD, EPR and UV-Vis spectroscopy were instrumental in providing insight into the mechanism of the catalyzed reaction. Vanadium oxides were also investigated as a cheaper alternative for the catalytic oxidation reaction. Phases of different vanadium oxides were synthesized by calcining NH4VO3 in air at different temperatures with an intention to investigate them for their catalytic activities. The catalyst obtained from calcination at 600⁰C was predominantly the orthorhombic phase of V2O5. Potential to immobilize the vanadium oxides was demonstrated using a silica support where NH4VO3 was impregnated onto silica and calcined in air at 600⁰C. The catalyst showed good potential in the oxidation of DBT to DBTO2, with 10 mg (43.9 μmol) of catalyst successfully converting 100% of 100 mg (0.543 mmol) DBT to DBTO2 using 2.0 mL (1.05 mmol) of t-BuOOH. The catalyst was also employed for a real fuel sample (diesel 500) with good results. The mechanistic aspects of vanadium oxides were also investigated in this study.
- Full Text:
- Date Issued: 2018
Microhabitat and biotic structure of stromatolite formations
- Authors: Weston, Ross-Lynne Alida
- Date: 2018
- Subjects: Stromatolites , Microbial mats Sedimentary structures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/17493 , vital:28358
- Description: Stromatolites are among the oldest types of microbial formations. In contrast to their ancient counterparts, many modern marine stromatolites have a coarser internal structure and host a diverse eukaryotic community. Different mesofabric structures are found within stromatolite formations which may provide microhabitat opportunities for organisms. Therefore, the first aim of this study was to investigate how the microalgal (including Bacillariophyta, Chlorophyta and cyanobacteria) community contributes towards the differences observed amongst mesofabric structures in terms of depth profiles and layering at representative stromatolites forming along the Port Elizabeth coastline in South Africa. The second aim was to investigate how the invertebrate community changes between mesofabric types. This was achieved by comparing the proportional abundances of each of the major microalgal classes between the different mesofabric types and depth profiles. Additionally, the invertebrates found within samples collected were identified and counted. These were related to site and environmental characteristics using multivariate modelling. Clear variability in terms of proportional abundance was apparent between microhabitats and with depth. Coarser, more-bioturbated types had a higher bacillariophyte biomass than smoother types. Invertebrate abundance was generally higher in coarser mats. However, one of the finer, well-laminated types had surprisingly more invertebrates than expected, but only in summer. The changes in microalgal and invertebrate community distribution varied seasonally. More microalgae were found in summer and more invertebrates were found in winter. Microalgae distribution was driven by mesofabric features whereas invertebrate distribution was attributed to resource changes. The implications of this study are in terms of the role of microalgae as ecosystem engineers in driving microhabitat differences. The microhabitats provide opportunities for colonisation by invertebrates.
- Full Text:
- Date Issued: 2018
- Authors: Weston, Ross-Lynne Alida
- Date: 2018
- Subjects: Stromatolites , Microbial mats Sedimentary structures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/17493 , vital:28358
- Description: Stromatolites are among the oldest types of microbial formations. In contrast to their ancient counterparts, many modern marine stromatolites have a coarser internal structure and host a diverse eukaryotic community. Different mesofabric structures are found within stromatolite formations which may provide microhabitat opportunities for organisms. Therefore, the first aim of this study was to investigate how the microalgal (including Bacillariophyta, Chlorophyta and cyanobacteria) community contributes towards the differences observed amongst mesofabric structures in terms of depth profiles and layering at representative stromatolites forming along the Port Elizabeth coastline in South Africa. The second aim was to investigate how the invertebrate community changes between mesofabric types. This was achieved by comparing the proportional abundances of each of the major microalgal classes between the different mesofabric types and depth profiles. Additionally, the invertebrates found within samples collected were identified and counted. These were related to site and environmental characteristics using multivariate modelling. Clear variability in terms of proportional abundance was apparent between microhabitats and with depth. Coarser, more-bioturbated types had a higher bacillariophyte biomass than smoother types. Invertebrate abundance was generally higher in coarser mats. However, one of the finer, well-laminated types had surprisingly more invertebrates than expected, but only in summer. The changes in microalgal and invertebrate community distribution varied seasonally. More microalgae were found in summer and more invertebrates were found in winter. Microalgae distribution was driven by mesofabric features whereas invertebrate distribution was attributed to resource changes. The implications of this study are in terms of the role of microalgae as ecosystem engineers in driving microhabitat differences. The microhabitats provide opportunities for colonisation by invertebrates.
- Full Text:
- Date Issued: 2018
Estuarine microalgal bloom dynamics at multiple temporal scales
- Authors: Lemley, Daniel Alan
- Date: 2018
- Subjects: Ecological integrity -- South Africa , Phytoplankton populations -- South Africa , Estuarine ecology -- South Africa , Freshwater algae -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21044 , vital:29431
- Description: Microalgal communities sustain the ecological functioning of estuarine ecosystems through the provision of numerous ecosystem services. As such, gaining an understanding of the processes that govern spatio-temporal patterns of phytoplankton communities is key to the effective management of these ecosystems. However, detailed investigations of estuarine phytoplankton dynamics, and particularly harmful algal blooms (HAB), are largely absent in South Africa. Therefore, geared at addressing these knowledge gaps, this study was initiated to elucidate the processes shaping estuarine phytoplankton bloom dynamics at multiple temporal scales. Initially, five permanently open estuaries, each with varying types and levels of anthropogenic pressures, were selected to test the efficacy of an estuarine eutrophic condition index to flow variability (‘Annual’ study). Results from this study highlighted the sensitivity of the proposed index to environmental perturbations (e.g. flood events), whilst also identifying freshwater inflow variability and anthropogenic disturbance as the key processes shaping microalgal responses at an annual scale. Next, the agriculturally-influenced Gamtoos and Sundays estuaries were further investigated to elucidate the role of natural and anthropogenic drivers in defining microalgal responses (‘Seasonal’ study). Despite similarities in nutrient input and temperature patterns, phytoplankton bloom conditions (> 20 μg Chl-a l-1) were episodic and flow-dependent in the Gamtoos Estuary, whilst those in the Sundays Estuary were persistent and seasonal. Related to its reduced hydrodynamic variability – i.e. consistent nutrient-rich baseflows and reduced propensity for flushing events – persistent undesirable disturbances were highlighted for the Sundays Estuary, including: summer bottom-water hypoxia (< 2 mg l-1) and exceptional proliferations (> 550 μg Chl-a l-1) of two HAB species (Heterosigma akashiwo and Heterocapsa rotundata). Finally, fine-scale ecological research was undertaken in the Sundays Estuary to identify the processes – abiotic and biotic – that facilitate HABs (‘Daily’ and ‘Hourly’ studies). Findings from the ‘Daily’ study, identified inorganic nutrient availability (i.e. nitrate and phosphate) and mesohaline conditions (ca. 10) as the key bottom-up controls influencing the magnitude and duration of spring/summer phytoplankton blooms. Additionally, bottom-water hypoxia was explicitly linked to the decay of a single HAB species. During the ‘Hourly’ investigation, four known HAB-forming species were recorded at bloom concentrations. Model results indicated that variability in temperature, salinity profiles and nitrate concentrations were significant in facilitating the occurrence of HAB species. Finally, local biotic interactions (e.g. interspecies competition, diel vertical migration and mixotrophy) were recognized as key mechanisms shaping phytoplankton communities. The persistent occurrence of HABs is a new feature in South African estuaries and continued research is needed to recommend management responses. Ultimately, this research highlights the multitude of processes at work shaping phytoplankton variability in estuaries. From a broad perspective (i.e. seasonal and annual), processes such as freshwater inflow regimes, degree of anthropogenic disturbance, as well as seasonal temperature and nutrient supply patterns are the key processes. At a more refined scale (i.e. hourly and daily), local processes including salinity preferences, nutrient availability, diel light cycles and internal biotic interactions are the key drivers organising phytoplankton dynamics. Given the potentially severe ecological consequences of disrupting natural phytoplankton dynamics (e.g. HABs), an element of ‘unpredictability’ should be restored to the hydrological and chemical makeup of highly-regulated estuaries to prevent the continued exacerbation of eutrophic symptoms.
- Full Text:
- Date Issued: 2018
- Authors: Lemley, Daniel Alan
- Date: 2018
- Subjects: Ecological integrity -- South Africa , Phytoplankton populations -- South Africa , Estuarine ecology -- South Africa , Freshwater algae -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21044 , vital:29431
- Description: Microalgal communities sustain the ecological functioning of estuarine ecosystems through the provision of numerous ecosystem services. As such, gaining an understanding of the processes that govern spatio-temporal patterns of phytoplankton communities is key to the effective management of these ecosystems. However, detailed investigations of estuarine phytoplankton dynamics, and particularly harmful algal blooms (HAB), are largely absent in South Africa. Therefore, geared at addressing these knowledge gaps, this study was initiated to elucidate the processes shaping estuarine phytoplankton bloom dynamics at multiple temporal scales. Initially, five permanently open estuaries, each with varying types and levels of anthropogenic pressures, were selected to test the efficacy of an estuarine eutrophic condition index to flow variability (‘Annual’ study). Results from this study highlighted the sensitivity of the proposed index to environmental perturbations (e.g. flood events), whilst also identifying freshwater inflow variability and anthropogenic disturbance as the key processes shaping microalgal responses at an annual scale. Next, the agriculturally-influenced Gamtoos and Sundays estuaries were further investigated to elucidate the role of natural and anthropogenic drivers in defining microalgal responses (‘Seasonal’ study). Despite similarities in nutrient input and temperature patterns, phytoplankton bloom conditions (> 20 μg Chl-a l-1) were episodic and flow-dependent in the Gamtoos Estuary, whilst those in the Sundays Estuary were persistent and seasonal. Related to its reduced hydrodynamic variability – i.e. consistent nutrient-rich baseflows and reduced propensity for flushing events – persistent undesirable disturbances were highlighted for the Sundays Estuary, including: summer bottom-water hypoxia (< 2 mg l-1) and exceptional proliferations (> 550 μg Chl-a l-1) of two HAB species (Heterosigma akashiwo and Heterocapsa rotundata). Finally, fine-scale ecological research was undertaken in the Sundays Estuary to identify the processes – abiotic and biotic – that facilitate HABs (‘Daily’ and ‘Hourly’ studies). Findings from the ‘Daily’ study, identified inorganic nutrient availability (i.e. nitrate and phosphate) and mesohaline conditions (ca. 10) as the key bottom-up controls influencing the magnitude and duration of spring/summer phytoplankton blooms. Additionally, bottom-water hypoxia was explicitly linked to the decay of a single HAB species. During the ‘Hourly’ investigation, four known HAB-forming species were recorded at bloom concentrations. Model results indicated that variability in temperature, salinity profiles and nitrate concentrations were significant in facilitating the occurrence of HAB species. Finally, local biotic interactions (e.g. interspecies competition, diel vertical migration and mixotrophy) were recognized as key mechanisms shaping phytoplankton communities. The persistent occurrence of HABs is a new feature in South African estuaries and continued research is needed to recommend management responses. Ultimately, this research highlights the multitude of processes at work shaping phytoplankton variability in estuaries. From a broad perspective (i.e. seasonal and annual), processes such as freshwater inflow regimes, degree of anthropogenic disturbance, as well as seasonal temperature and nutrient supply patterns are the key processes. At a more refined scale (i.e. hourly and daily), local processes including salinity preferences, nutrient availability, diel light cycles and internal biotic interactions are the key drivers organising phytoplankton dynamics. Given the potentially severe ecological consequences of disrupting natural phytoplankton dynamics (e.g. HABs), an element of ‘unpredictability’ should be restored to the hydrological and chemical makeup of highly-regulated estuaries to prevent the continued exacerbation of eutrophic symptoms.
- Full Text:
- Date Issued: 2018
Productivity and resilience of intertidal resources available to extant human foragers on South Africa’s cape south coast: behavioural implications for early Homo sapiens
- Authors: De Vynck, Jan Carlo
- Date: 2018
- Subjects: Marine invertebrates -- South Africa -- Prehistory , Shellfish -- South Africa -- Prehistory Fishes -- Behavior
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30172 , vital:30854
- Description: Studies of pre-historic lifestyles can be limited by a lack of preserved material and sites and resource misinterpretation is augmented by the vagueness of pre-historic subsistence lifestyles. The present can inform the past and this study‟s ethnoarchaeological approach, through modern analogues, examines productivity, resilience and temporal and spatial size variation of intertidal invertebrates on the Cape‟s south coast. O‟Connell (1995) defined the mutualistic relationship between ethnography and archaeology thus: “Archaeology‟s ultimate aim is understanding past human behaviour by patterns in the form and distribution of objects made or modified by humans in the past and knowledge of human behaviour and its material consequences in the present. The first provides direct evidence of past behaviour; the second, a basis for interpreting that evidence”. Coastal archaeology, specifically the contribution of coastal resources to pre-historic subsistence, has gained increased global interest (Erlandson, 2001; Erlandson and Rick, 2008; Codding et al., 2014). Apart from the high representation of preserved shell in coastal archaeological sites, interest in intertidal resources has also been sparked by their economic value compared to terrestrial resource options. Intertidal resources offer low-risk protein acquisition whereas terrestrial protein can be dangerous to procure. However, in the past intertidal subsistence was thought to offer low caloric yields or unfavourable cost-to-benefit relationships until Meehan (1982) convincingly demonstrated the potential caloric „profit‟ possible from coastal foraging; the Anbarra women of northern Australia were contributing significantly to the overall caloric requirements of those people. In South Africa, two possible evolutionary behavioural hypotheses arose from the addition of intertidal resources to pre-historic diets. As background, it is important to note that the oldest global evidence for shellfish procurement [164 000 years ago (ka)] is found on the Cape‟s south coast (Jerardino and Marean, 2010) and the evidence is enriched by other sites from around 110 ka (Voight, 1973; Thackeray, 1988; Henshilwood et al., 2001; Langejans et al., 2012). Furthermore, this period coincides with the emergence of cognitively modern Homo sapiens as evidenced in xx the palaeoarchives from many sites in the region (d‟Errico et al., 2005; Marean et al., 2007; Wurz, 2008; Henshilwood et al., 2009; Watts, 2010; d‟Errico et al., 2012). These features have greatly enhanced the significance and importance of this area to understanding human evolution. The two hypotheses are centred around the effect that intertidal resources could have had on the emergence of our unique species. The addition of a nutritionally beneficial resource to cognitive development is seen by some (Broadhurst et al., 2002; Parkington, 2010) to have driven this cognitive evolution, where others suggest that resources which are both productive and dependable create pro-social proclivities that drive cognitive development (Whitaker and Byrd, 2014; Marean, 2014) and facilitate the migration of humans (Fa, 2008; Codding et al., 2014). Both hypotheses argue that resources on the Cape‟s coast must have been productive and had to be a frequent addition to diet to underpin brain development. The increased frequency of coastal resource procurement or coastal adaptation is therefore seen as an attribute of, or requisite for our species. This thesis attempts to shed light on the contribution of intertidal resources to prehistoric ‒ especially Middle Stone Age (MSA) – subsistence economy on the Cape‟s south coast and the possible effect thereof on human behavioural evolution. The first aim was to investigate the spatial productivity (once-off patch caloric profitability in numerous patches) of intertidal resources and the environmental and behavioural variables affecting it. The second aim was to investigate the resilience of caloric profitability (numerous harvests in the same patch) of these resources to sustained human predation. Productivity itself cannot affect human temporal social dynamics if the resource is swiftly depleted within a specific patch after one or a few harvests. Archaeological records that show the body size of shellfish decreasing over time are usually interpreted to indicate intensive exploitation and the depletion of these resources, but there may be other explanations for such patterns. Therefore, the third aim was to compare temporal and spatial variations in the body size of Turbo sarmaticus in the MSA, Later Stone Age (LSA) and the present day, in order to investigate whether such variations result from human exploitation or environmental changes.
- Full Text:
- Date Issued: 2018
- Authors: De Vynck, Jan Carlo
- Date: 2018
- Subjects: Marine invertebrates -- South Africa -- Prehistory , Shellfish -- South Africa -- Prehistory Fishes -- Behavior
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30172 , vital:30854
- Description: Studies of pre-historic lifestyles can be limited by a lack of preserved material and sites and resource misinterpretation is augmented by the vagueness of pre-historic subsistence lifestyles. The present can inform the past and this study‟s ethnoarchaeological approach, through modern analogues, examines productivity, resilience and temporal and spatial size variation of intertidal invertebrates on the Cape‟s south coast. O‟Connell (1995) defined the mutualistic relationship between ethnography and archaeology thus: “Archaeology‟s ultimate aim is understanding past human behaviour by patterns in the form and distribution of objects made or modified by humans in the past and knowledge of human behaviour and its material consequences in the present. The first provides direct evidence of past behaviour; the second, a basis for interpreting that evidence”. Coastal archaeology, specifically the contribution of coastal resources to pre-historic subsistence, has gained increased global interest (Erlandson, 2001; Erlandson and Rick, 2008; Codding et al., 2014). Apart from the high representation of preserved shell in coastal archaeological sites, interest in intertidal resources has also been sparked by their economic value compared to terrestrial resource options. Intertidal resources offer low-risk protein acquisition whereas terrestrial protein can be dangerous to procure. However, in the past intertidal subsistence was thought to offer low caloric yields or unfavourable cost-to-benefit relationships until Meehan (1982) convincingly demonstrated the potential caloric „profit‟ possible from coastal foraging; the Anbarra women of northern Australia were contributing significantly to the overall caloric requirements of those people. In South Africa, two possible evolutionary behavioural hypotheses arose from the addition of intertidal resources to pre-historic diets. As background, it is important to note that the oldest global evidence for shellfish procurement [164 000 years ago (ka)] is found on the Cape‟s south coast (Jerardino and Marean, 2010) and the evidence is enriched by other sites from around 110 ka (Voight, 1973; Thackeray, 1988; Henshilwood et al., 2001; Langejans et al., 2012). Furthermore, this period coincides with the emergence of cognitively modern Homo sapiens as evidenced in xx the palaeoarchives from many sites in the region (d‟Errico et al., 2005; Marean et al., 2007; Wurz, 2008; Henshilwood et al., 2009; Watts, 2010; d‟Errico et al., 2012). These features have greatly enhanced the significance and importance of this area to understanding human evolution. The two hypotheses are centred around the effect that intertidal resources could have had on the emergence of our unique species. The addition of a nutritionally beneficial resource to cognitive development is seen by some (Broadhurst et al., 2002; Parkington, 2010) to have driven this cognitive evolution, where others suggest that resources which are both productive and dependable create pro-social proclivities that drive cognitive development (Whitaker and Byrd, 2014; Marean, 2014) and facilitate the migration of humans (Fa, 2008; Codding et al., 2014). Both hypotheses argue that resources on the Cape‟s coast must have been productive and had to be a frequent addition to diet to underpin brain development. The increased frequency of coastal resource procurement or coastal adaptation is therefore seen as an attribute of, or requisite for our species. This thesis attempts to shed light on the contribution of intertidal resources to prehistoric ‒ especially Middle Stone Age (MSA) – subsistence economy on the Cape‟s south coast and the possible effect thereof on human behavioural evolution. The first aim was to investigate the spatial productivity (once-off patch caloric profitability in numerous patches) of intertidal resources and the environmental and behavioural variables affecting it. The second aim was to investigate the resilience of caloric profitability (numerous harvests in the same patch) of these resources to sustained human predation. Productivity itself cannot affect human temporal social dynamics if the resource is swiftly depleted within a specific patch after one or a few harvests. Archaeological records that show the body size of shellfish decreasing over time are usually interpreted to indicate intensive exploitation and the depletion of these resources, but there may be other explanations for such patterns. Therefore, the third aim was to compare temporal and spatial variations in the body size of Turbo sarmaticus in the MSA, Later Stone Age (LSA) and the present day, in order to investigate whether such variations result from human exploitation or environmental changes.
- Full Text:
- Date Issued: 2018
Assessment of the host potential of TETROL [(+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-TETROL] for the separation of isomers and related compounds
- Authors: Dorfling, Sasha-Lee
- Date: 2018
- Subjects: Chemistry, Organic , Thermal analysis Hydrogen bonding
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/22060 , vital:29817
- Description: In this study, we investigated the potential of a host compound, (+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-tetrol (TETROL), for use in the separation of isomers and related compounds using host-guest chemistry. The synthesis of this host was carried out using a standard Grignard procedure, reacting naturally-occurring optically active tartaric acid with phenylmagnesium bromide. The feasibility of this host for separating isomers and structurally-related compounds was investigated by recrystallizing it from various potential cyclic, aromatic and aliphatic guest compounds. The extent of host inclusion and guest separation were determined using 1H-NMR spectroscopy and GCMS analyses. Competition studies were conducted to establish the selectivity of TETROL for the various guest species and whether this host would be able to discriminate between them. In this instance, the host was recrystallized from equimolar amounts of binary, ternary, quaternary or quinary mixtures of the guests present in each target study. Subsequent binary or ternary competitions were conducted where the molar ratios of the guest species were varied beyond equimolar, and the guest selectivity of TETROL thus evaluated by means of selectivity profiles. Further analyses included single crystal X-ray diffraction (SCXRD), thermal analysis and Hirshfeld surface analysis. Any crystalline inclusion complex formed between host and guest, with suitable crystal quality, was analysed using SCXRD in order to determine the nature of any significant host–guest interactions present. Thermogravimetric and differential scanning calorimetry experiments provided further insight into complex stability by analysing the thermal events experienced by the complexes as they were heated at 10 °C/min. The data obtained from Hirshfeld surface analyses were used to determine whether host selectivity and/or thermal stability of the complexes were related to the number and types of interactions, observed from SCXRD, between host and guest. The ability of TETROL to discriminate between related compounds was favourable. This host proved to have selective preference for aniline over its methylated derivatives, N-methylaniline and N,N-dimethylaniline. It was also selective for cyclohexylamine over cyclohexanol and cyclohexanone, and discriminated against the pyridine, piperidine and dioxane heterocyclics in favour of morpholine. Furthermore, this host was successful in the selective separation of isomers; for example, it selectively showed discrimination between the three toluidine isomers (p-toluidine > m-toluidine > o-toluidine) and the cresols (p-cresol > m-cresol > o-cresol). Each guest mixture was selected based on data from experiments using either the industrial significance of its separation or because the mixture would add to the knowledge base of the host compound’s preferences and selectivities. In a separate study, TETROL and its derivative, (–)-(2R,3R)-2,3-dimethoxy-1,1,4,4- tetraphenylbutane-1,4-diol (DMT), were also allowed to compete for the inclusion of the guest cyclohexanone, where TETROL demonstrated superior ability. This host, in addition, showed potential for the separation of cis- and trans- 2-methylcyclohexanol.
- Full Text:
- Date Issued: 2018
- Authors: Dorfling, Sasha-Lee
- Date: 2018
- Subjects: Chemistry, Organic , Thermal analysis Hydrogen bonding
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/22060 , vital:29817
- Description: In this study, we investigated the potential of a host compound, (+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-tetrol (TETROL), for use in the separation of isomers and related compounds using host-guest chemistry. The synthesis of this host was carried out using a standard Grignard procedure, reacting naturally-occurring optically active tartaric acid with phenylmagnesium bromide. The feasibility of this host for separating isomers and structurally-related compounds was investigated by recrystallizing it from various potential cyclic, aromatic and aliphatic guest compounds. The extent of host inclusion and guest separation were determined using 1H-NMR spectroscopy and GCMS analyses. Competition studies were conducted to establish the selectivity of TETROL for the various guest species and whether this host would be able to discriminate between them. In this instance, the host was recrystallized from equimolar amounts of binary, ternary, quaternary or quinary mixtures of the guests present in each target study. Subsequent binary or ternary competitions were conducted where the molar ratios of the guest species were varied beyond equimolar, and the guest selectivity of TETROL thus evaluated by means of selectivity profiles. Further analyses included single crystal X-ray diffraction (SCXRD), thermal analysis and Hirshfeld surface analysis. Any crystalline inclusion complex formed between host and guest, with suitable crystal quality, was analysed using SCXRD in order to determine the nature of any significant host–guest interactions present. Thermogravimetric and differential scanning calorimetry experiments provided further insight into complex stability by analysing the thermal events experienced by the complexes as they were heated at 10 °C/min. The data obtained from Hirshfeld surface analyses were used to determine whether host selectivity and/or thermal stability of the complexes were related to the number and types of interactions, observed from SCXRD, between host and guest. The ability of TETROL to discriminate between related compounds was favourable. This host proved to have selective preference for aniline over its methylated derivatives, N-methylaniline and N,N-dimethylaniline. It was also selective for cyclohexylamine over cyclohexanol and cyclohexanone, and discriminated against the pyridine, piperidine and dioxane heterocyclics in favour of morpholine. Furthermore, this host was successful in the selective separation of isomers; for example, it selectively showed discrimination between the three toluidine isomers (p-toluidine > m-toluidine > o-toluidine) and the cresols (p-cresol > m-cresol > o-cresol). Each guest mixture was selected based on data from experiments using either the industrial significance of its separation or because the mixture would add to the knowledge base of the host compound’s preferences and selectivities. In a separate study, TETROL and its derivative, (–)-(2R,3R)-2,3-dimethoxy-1,1,4,4- tetraphenylbutane-1,4-diol (DMT), were also allowed to compete for the inclusion of the guest cyclohexanone, where TETROL demonstrated superior ability. This host, in addition, showed potential for the separation of cis- and trans- 2-methylcyclohexanol.
- Full Text:
- Date Issued: 2018
Electrospinning of carbon nanofibers for investigating the behaviour of lead electrodeposits on the carbon surface
- Authors: Madikane, Zipporah Kayakazi
- Date: 2018
- Subjects: Materials science , Nanotechnology Nanostructured materials
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/31622 , vital:31629
- Description: Over the years, the lead acid battery’s performance in modern vehicles has improved considerably to adapt to the new demands of vehicle electronics and start-stop applications. Although vehicles are valuable for commuting, their high volume of usage has resulted in an increase of fuel prices and emitted carbon dioxide (CO2). Due to the drastic global warming concerns, vehicle manufactures have been pressured into developing a variety of hybrid electric vehicles (HEV) that would reduce their fuel consumption and thereby reduced CO2 emission. The HEVs construction requires the implementation of advanced battery systems that operate under high rate partial state of charge (HRPSoC) conditions. During battery’s discharge, the Pb contained on the negative plate is oxidised to insulating PbSO4 crystals. When the battery is charged, the PbSO4 is reduced back to Pb. With prolonged use at HRPSoC, the battery undergoes an irreversible process wherein small PbSO4 crystallites dissolve and precipitate into large passivating PbSO4 crystals that accumulate as a dense layer on the surface of the negative plate. This layer restricts the diffusion of the H2SO4 electrolyte into the reaction sites for further reactions, resulting in the battery to fail prematurely. Research has shown that the inclusion of appropriate carbon materials on the negative active material (NAM) improves the performance of the batteries in HRPSoC cycling. This study looked at the making of some of the carbon-based additives by using relatively cheap materials such as polyvinyl alcohol (PVA) polymer with a sucrose blend in ratios of 75:25, 50:50 and 25:75, respectively. The blended materials were electrospun into PVA/Sucrose nanofibers. These were further stabilised by iodine and carbonised at 500 °C in a tube furnace. The properties of the carbon fibres were investigated by a number of analytical techniques such as TGA, DSC, XRD, BET, FTIR and SEM. The obtained carbon fibre surfaces were further electroplated with Pb in order to investigate the chemical bonding, the relationship between the carbon-based material interface and the Pb was investigated by SEM and CV techniques. The results of the study demonstrated that the varied ratios of the PVA/Sucrose nanofibers influenced the differences in their respective chemical and physical properties. Thermal analysis demonstrated that at approximately 500 °C, the final residue of the PVA/Sucrose nanofibers decreased as the sucrose content decreased. iii Carbonisation of the nanofibers was confirmed by the structural changes corresponding to the disappearance of the major bonds of PVA and sucrose materials, as well as by the appearance of C=C stretches. Carbonisation was also confirmed by the XRD diffractogram that demonstrated two broad amorphous peaks that corresponded to the planes of the graphite structure. The broadness of the peaks confirmed the amorphous nature of the carbon fibres. The morphological studies demonstrated that upon blending PVA with increasing quantities of sucrose, the uniformity of the fibres was altered into beaded fibrous structures with large diameters due to the increased viscosity of the electrospinning solution. The behaviour of the Pb deposits on the carbon surfaces was influenced by the high quantity of PVA present on the blend and the fibrous structure of the carbonised material. The Pb had high affinity towards the fibrous carbon derived from 75:25 as it deposited widely across the surface. Whereas, the absence of Pb deposits on the surface of the carbon derived from the 25:75 ratio was an indication that the high sucrose content and the bulky non-fibrous structure hindered the electrodeposition. The electrochemical study findings demonstrated that the bare Pb electrode had two distinct current peaks corresponding to the anodic (discharge) and cathodic (charge) reactions of Pb/PbSO4. Upon the inclusion of the electrospun carbon materials on the Pb electrode, the intensities of the anodic and cathodic peaks were reduced. Implying that none of the carbon materials enhanced the electrochemical redox reactions of the Pb/PbSO4 couple in the H2SO4 electrolyte.
- Full Text:
- Date Issued: 2018
- Authors: Madikane, Zipporah Kayakazi
- Date: 2018
- Subjects: Materials science , Nanotechnology Nanostructured materials
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/31622 , vital:31629
- Description: Over the years, the lead acid battery’s performance in modern vehicles has improved considerably to adapt to the new demands of vehicle electronics and start-stop applications. Although vehicles are valuable for commuting, their high volume of usage has resulted in an increase of fuel prices and emitted carbon dioxide (CO2). Due to the drastic global warming concerns, vehicle manufactures have been pressured into developing a variety of hybrid electric vehicles (HEV) that would reduce their fuel consumption and thereby reduced CO2 emission. The HEVs construction requires the implementation of advanced battery systems that operate under high rate partial state of charge (HRPSoC) conditions. During battery’s discharge, the Pb contained on the negative plate is oxidised to insulating PbSO4 crystals. When the battery is charged, the PbSO4 is reduced back to Pb. With prolonged use at HRPSoC, the battery undergoes an irreversible process wherein small PbSO4 crystallites dissolve and precipitate into large passivating PbSO4 crystals that accumulate as a dense layer on the surface of the negative plate. This layer restricts the diffusion of the H2SO4 electrolyte into the reaction sites for further reactions, resulting in the battery to fail prematurely. Research has shown that the inclusion of appropriate carbon materials on the negative active material (NAM) improves the performance of the batteries in HRPSoC cycling. This study looked at the making of some of the carbon-based additives by using relatively cheap materials such as polyvinyl alcohol (PVA) polymer with a sucrose blend in ratios of 75:25, 50:50 and 25:75, respectively. The blended materials were electrospun into PVA/Sucrose nanofibers. These were further stabilised by iodine and carbonised at 500 °C in a tube furnace. The properties of the carbon fibres were investigated by a number of analytical techniques such as TGA, DSC, XRD, BET, FTIR and SEM. The obtained carbon fibre surfaces were further electroplated with Pb in order to investigate the chemical bonding, the relationship between the carbon-based material interface and the Pb was investigated by SEM and CV techniques. The results of the study demonstrated that the varied ratios of the PVA/Sucrose nanofibers influenced the differences in their respective chemical and physical properties. Thermal analysis demonstrated that at approximately 500 °C, the final residue of the PVA/Sucrose nanofibers decreased as the sucrose content decreased. iii Carbonisation of the nanofibers was confirmed by the structural changes corresponding to the disappearance of the major bonds of PVA and sucrose materials, as well as by the appearance of C=C stretches. Carbonisation was also confirmed by the XRD diffractogram that demonstrated two broad amorphous peaks that corresponded to the planes of the graphite structure. The broadness of the peaks confirmed the amorphous nature of the carbon fibres. The morphological studies demonstrated that upon blending PVA with increasing quantities of sucrose, the uniformity of the fibres was altered into beaded fibrous structures with large diameters due to the increased viscosity of the electrospinning solution. The behaviour of the Pb deposits on the carbon surfaces was influenced by the high quantity of PVA present on the blend and the fibrous structure of the carbonised material. The Pb had high affinity towards the fibrous carbon derived from 75:25 as it deposited widely across the surface. Whereas, the absence of Pb deposits on the surface of the carbon derived from the 25:75 ratio was an indication that the high sucrose content and the bulky non-fibrous structure hindered the electrodeposition. The electrochemical study findings demonstrated that the bare Pb electrode had two distinct current peaks corresponding to the anodic (discharge) and cathodic (charge) reactions of Pb/PbSO4. Upon the inclusion of the electrospun carbon materials on the Pb electrode, the intensities of the anodic and cathodic peaks were reduced. Implying that none of the carbon materials enhanced the electrochemical redox reactions of the Pb/PbSO4 couple in the H2SO4 electrolyte.
- Full Text:
- Date Issued: 2018
On the development of ZnO nanorods on silicon substrate for light-emitting diode applications
- Djiokap, Stive Roussel Tankio
- Authors: Djiokap, Stive Roussel Tankio
- Date: 2018
- Subjects: Zinc oxide , Chemical reactions , Compound semiconductors
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/29973 , vital:30802
- Description: The interest in zinc oxide (ZnO), a promising material for blue/ultraviolet light emitting devices, arises from its large exciton binding energy (60 meV). The main challenge associated with this promising compound semiconductor, however, arises from the difficulty to achieve stable and/or reproducible p-type doping. Since silicon (Si) technology still dominates the semiconductor industry, the objective of this thesis is to probe into the possibility of using ZnO nanorods (NRs) on p-type silicon for opto-electronic devices. ZnO NRs have been grown on seeded Si, as well as on nickel oxide (NiO) and aluminum nitride (AlN) coated Si, using a two-step chemical bath deposition (CBD) process. Various techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), photoluminescence (PL) spectroscopy and transmission electron microscopy (TEM), have been used to characterize the samples. The electrical characteristics of the heterojunction between the substrate and the ZnO nanostructures were evaluated by current-voltage (I-V) and capacitance-voltage (C-V) measurements. SEM and XRD studies have confirmed that, irrespective of the orientation of the Si substrate (Si (100) and Si (111)), the two-step CBD process yielded NRs that crystallised in the wurtzite structure and exhibited a hexagonal shape. Most of the rods developed perpendicularly to the surface of the substrate, with the orientation and distribution of the rods dictated by the seed layer density. Similarly, irrespective of the substrate, the luminescence of the ZnO nanostructures is dominated by near band edge (NBE) emission in the UV region (~ 3.29 eV) and deep level emission (DLE) in the visible region (2 eV to 2.6 eV). Annealing at moderate temperatures (~ 300 °C) increased the NBE emission and decreased the DLE. The removal of surface adsorbed impurities and enhanced defect passivation by hydrogen are responsible for these changes. The diode characteristics of the ZnO/Si heterojunction was studied by I-V and C-V measurements. Rectification was observed when the Si substrate had a relatively low acceptor density of ~1016 cm-3, while diodes produced on substrate with p ~1018 cm-3 were ohmic. From the C-V analysis the donor density in the ZnO was deduced to be ~1018 cm-3. In the case of rectifying junctions, thermionic emission did not dominate the charge transport. The carrier transport mechanism was therefore probed by the temperature dependent I-V xiii measurements (100 K to 295 K). Defect-assisted multistep tunneling was deduced to dominate in the n-ZnO/p-Si diodes at low forward bias. The band alignment between n-ZnO and p-Si predicts a much smaller barrier for electrons than for holes at the interface, which results in recombination on the Si side of the junction for a forward-biased diode. NiO intermediate layers (formed on Si by the thermal oxidation of Ni) were used to reduce electron injection from ZnO into Si. Scanning probe microscopy (SPM) and XRD analysis showed that while the grain size of the poly-crystalline NiO increased with NiO film thickness, the orientation and distribution of the subsequently grown ZnO nanorods were unaffected by the underlying NiO layer. Also, the photoluminescence response of the ZnO rods remained unchanged. I-V measurements did illustrate rectifying behaviour, with both the forward and reverse currents strongly decreased due to the resistive nature of the NiO. In another attempt at confining electrons to the ZnO side of the junction, AlN-coated Si (111) was used as a substrate for ZnO nanorods. CBD parameters that normally yield nanorods resulted in a plate-like architecture of the ZnO. By modifying the ZnO seed density on the AlN/Si substrate, the rod-like morphology could be recovered. Both the forward and reverse current decreased in these diodes. From studies aimed at identifying the transport mechanism it was concluded that trap-assisted tunnelling, resulting from a high density of defects in the seed layer, dominates in these devices. In conclusion, while no ZnO electroluminescence could be achieved from any of the devices, this study provides insight into the transport mechanisms in n-ZnO/barrier/p-Si heterostructures and highlights the importance of the heterointerface quality for light emitting devices.
- Full Text:
- Date Issued: 2018
- Authors: Djiokap, Stive Roussel Tankio
- Date: 2018
- Subjects: Zinc oxide , Chemical reactions , Compound semiconductors
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/29973 , vital:30802
- Description: The interest in zinc oxide (ZnO), a promising material for blue/ultraviolet light emitting devices, arises from its large exciton binding energy (60 meV). The main challenge associated with this promising compound semiconductor, however, arises from the difficulty to achieve stable and/or reproducible p-type doping. Since silicon (Si) technology still dominates the semiconductor industry, the objective of this thesis is to probe into the possibility of using ZnO nanorods (NRs) on p-type silicon for opto-electronic devices. ZnO NRs have been grown on seeded Si, as well as on nickel oxide (NiO) and aluminum nitride (AlN) coated Si, using a two-step chemical bath deposition (CBD) process. Various techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), photoluminescence (PL) spectroscopy and transmission electron microscopy (TEM), have been used to characterize the samples. The electrical characteristics of the heterojunction between the substrate and the ZnO nanostructures were evaluated by current-voltage (I-V) and capacitance-voltage (C-V) measurements. SEM and XRD studies have confirmed that, irrespective of the orientation of the Si substrate (Si (100) and Si (111)), the two-step CBD process yielded NRs that crystallised in the wurtzite structure and exhibited a hexagonal shape. Most of the rods developed perpendicularly to the surface of the substrate, with the orientation and distribution of the rods dictated by the seed layer density. Similarly, irrespective of the substrate, the luminescence of the ZnO nanostructures is dominated by near band edge (NBE) emission in the UV region (~ 3.29 eV) and deep level emission (DLE) in the visible region (2 eV to 2.6 eV). Annealing at moderate temperatures (~ 300 °C) increased the NBE emission and decreased the DLE. The removal of surface adsorbed impurities and enhanced defect passivation by hydrogen are responsible for these changes. The diode characteristics of the ZnO/Si heterojunction was studied by I-V and C-V measurements. Rectification was observed when the Si substrate had a relatively low acceptor density of ~1016 cm-3, while diodes produced on substrate with p ~1018 cm-3 were ohmic. From the C-V analysis the donor density in the ZnO was deduced to be ~1018 cm-3. In the case of rectifying junctions, thermionic emission did not dominate the charge transport. The carrier transport mechanism was therefore probed by the temperature dependent I-V xiii measurements (100 K to 295 K). Defect-assisted multistep tunneling was deduced to dominate in the n-ZnO/p-Si diodes at low forward bias. The band alignment between n-ZnO and p-Si predicts a much smaller barrier for electrons than for holes at the interface, which results in recombination on the Si side of the junction for a forward-biased diode. NiO intermediate layers (formed on Si by the thermal oxidation of Ni) were used to reduce electron injection from ZnO into Si. Scanning probe microscopy (SPM) and XRD analysis showed that while the grain size of the poly-crystalline NiO increased with NiO film thickness, the orientation and distribution of the subsequently grown ZnO nanorods were unaffected by the underlying NiO layer. Also, the photoluminescence response of the ZnO rods remained unchanged. I-V measurements did illustrate rectifying behaviour, with both the forward and reverse currents strongly decreased due to the resistive nature of the NiO. In another attempt at confining electrons to the ZnO side of the junction, AlN-coated Si (111) was used as a substrate for ZnO nanorods. CBD parameters that normally yield nanorods resulted in a plate-like architecture of the ZnO. By modifying the ZnO seed density on the AlN/Si substrate, the rod-like morphology could be recovered. Both the forward and reverse current decreased in these diodes. From studies aimed at identifying the transport mechanism it was concluded that trap-assisted tunnelling, resulting from a high density of defects in the seed layer, dominates in these devices. In conclusion, while no ZnO electroluminescence could be achieved from any of the devices, this study provides insight into the transport mechanisms in n-ZnO/barrier/p-Si heterostructures and highlights the importance of the heterointerface quality for light emitting devices.
- Full Text:
- Date Issued: 2018
Biological activity of macrofungi in South Africa against respiratory and lung disease
- Authors: Didloff, Jenske
- Date: 2018
- Subjects: Macrofungi , Microbiology Medical microbiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30138 , vital:30835
- Description: Macrofungi represent an untapped source of natural bioactive compounds for various diseases, which have been targeted as potential therapeutic agents. The medicinal uses of macrofungi discovered to date include anticancer, antidiabetic, antioxidant, antimicrobial, and immunomodulatory properties. The knowledge regarding the medicinal uses of macrofungi in Africa is limited; however, it is believed that Africa may contain a large number of unidentified species of macrofungi. The objectives of this study were to: (i) screen the macrofungal extracts for antimicrobial activity against microorganisms responsible for respiratory diseases (e.g. Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Mycobacterium tuberculosis, HIV-1), (ii) determine the effect of macrofungal extracts on bacterial morphology, (iii) investigate the cytotoxicity of macrofungal extracts against human lung carcinoma cells, and to elucidate the mechanism/s of action of cytotoxicity/anticancer activity. In vitro bioassays for antimicrobial activity included: ρ-iodonitrotetrazolium chloride assays and microplate alamar blue assay (MABA), while the HIV-1 reverse transcriptase colorimetric ELISA and HIV-1 protease fluorometric assay kits were used for anti-HIV activity. Cytotoxicity of the macrofungal species against A549 lung cancer cell line was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and the IC50 values determined for the most cytotoxic macrofungal spp. The mechanism of cytotoxicity was investigated by cell cycle analysis and fluorescent staining to observe morphological and biochemical changes (i.e. acridine orange, caspase activation). Ethanol extracts of Amanita foetidissima, Gymnopilus junonius, Pisolithus tinctorius, Fomitopsis lilacinogilva, Stereum hirsutum and Pycnoporus sanguineus showed higher antimicrobial activity against the Gram-positive bacteria than aqueous extracts, with S. pneumoniae being the most susceptible. The ethanol extracts of Agaricus campestris, Chlorophyllum molybdites, Gymnopilus penetrans, Pseudophaeolus baudonii and Laetiporus sulphureus exhibited anti-TB (tuberculosis) activity with minimum inhibitory concentrations (MICs) ranging between 500-1,000 μg/mL. C. molybdites ethanol extract inhibited HIV-1 protease activity (IC50: 49.7 μg/mL). The macrofungal extracts did not inhibit HIV-1 reverse transcriptase activity. Ethanol extracts of F. lilacinogilva, G. junonius, P. sanguineus and the aqueous extract iv of P. baudonii were cytotoxic against A549 lung cancer cells at IC50 values of 69.2±3.6, 57±5, 7.4±1.1 and 53.6±1.1 μg/mL, respectively. Cell cycle arrest was observed in the G2 phase for both P. sanguineus and P. baudonii, and G2/M and early M phases for G. junonius and F. lilacinogilva, respectively. Apoptosis induced by macrofungal extracts was confirmed by fluorescent staining. Morphological and biochemical changes included chromatin condensation, membrane blebbing, loss of cytoskeletal structure, caspase activation and phosphatidylserine translocation. This study demonstrates the biological activities of selected macrofungal extracts and their potential mechanisms of action. Isolation and identification of active compounds require further analysis.
- Full Text:
- Date Issued: 2018
- Authors: Didloff, Jenske
- Date: 2018
- Subjects: Macrofungi , Microbiology Medical microbiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30138 , vital:30835
- Description: Macrofungi represent an untapped source of natural bioactive compounds for various diseases, which have been targeted as potential therapeutic agents. The medicinal uses of macrofungi discovered to date include anticancer, antidiabetic, antioxidant, antimicrobial, and immunomodulatory properties. The knowledge regarding the medicinal uses of macrofungi in Africa is limited; however, it is believed that Africa may contain a large number of unidentified species of macrofungi. The objectives of this study were to: (i) screen the macrofungal extracts for antimicrobial activity against microorganisms responsible for respiratory diseases (e.g. Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Mycobacterium tuberculosis, HIV-1), (ii) determine the effect of macrofungal extracts on bacterial morphology, (iii) investigate the cytotoxicity of macrofungal extracts against human lung carcinoma cells, and to elucidate the mechanism/s of action of cytotoxicity/anticancer activity. In vitro bioassays for antimicrobial activity included: ρ-iodonitrotetrazolium chloride assays and microplate alamar blue assay (MABA), while the HIV-1 reverse transcriptase colorimetric ELISA and HIV-1 protease fluorometric assay kits were used for anti-HIV activity. Cytotoxicity of the macrofungal species against A549 lung cancer cell line was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and the IC50 values determined for the most cytotoxic macrofungal spp. The mechanism of cytotoxicity was investigated by cell cycle analysis and fluorescent staining to observe morphological and biochemical changes (i.e. acridine orange, caspase activation). Ethanol extracts of Amanita foetidissima, Gymnopilus junonius, Pisolithus tinctorius, Fomitopsis lilacinogilva, Stereum hirsutum and Pycnoporus sanguineus showed higher antimicrobial activity against the Gram-positive bacteria than aqueous extracts, with S. pneumoniae being the most susceptible. The ethanol extracts of Agaricus campestris, Chlorophyllum molybdites, Gymnopilus penetrans, Pseudophaeolus baudonii and Laetiporus sulphureus exhibited anti-TB (tuberculosis) activity with minimum inhibitory concentrations (MICs) ranging between 500-1,000 μg/mL. C. molybdites ethanol extract inhibited HIV-1 protease activity (IC50: 49.7 μg/mL). The macrofungal extracts did not inhibit HIV-1 reverse transcriptase activity. Ethanol extracts of F. lilacinogilva, G. junonius, P. sanguineus and the aqueous extract iv of P. baudonii were cytotoxic against A549 lung cancer cells at IC50 values of 69.2±3.6, 57±5, 7.4±1.1 and 53.6±1.1 μg/mL, respectively. Cell cycle arrest was observed in the G2 phase for both P. sanguineus and P. baudonii, and G2/M and early M phases for G. junonius and F. lilacinogilva, respectively. Apoptosis induced by macrofungal extracts was confirmed by fluorescent staining. Morphological and biochemical changes included chromatin condensation, membrane blebbing, loss of cytoskeletal structure, caspase activation and phosphatidylserine translocation. This study demonstrates the biological activities of selected macrofungal extracts and their potential mechanisms of action. Isolation and identification of active compounds require further analysis.
- Full Text:
- Date Issued: 2018
A seismic characterization of the crustal structure underlying the offshore Natal valley within the south-west Indian Ocean
- Authors: Moorcroft, David
- Date: 2018
- Subjects: Seismology , Seismology -- Kwa Zulu Natal -- Research Coasts -- Kwa Zulu Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/22387 , vital:29965
- Description: The Natal Valley is a ~1000 km long, elongated depression in the seafloor, located within the south-west Indian Ocean, between the coastline of south-east Africa and the offshore aseismic Mozambique Ridge. Knowledge of its present-day crustal structure is important to reconstruct the Gondwana supercontinent break-up history and the concurrent opening of the Indian Ocean, which started during the Jurassic, some ~180 – 150 million years ago. Major geological events relating to this supercontinent break-up including continental rifting, magmatism, the emplacement of extensive oceanic flood basalt as submarine plateaus, vertical processes associated with tectonic uplift, flexural subsidence of the margin and relative global sea-level change are recorded within the crust underlying the south-west Indian Ocean. Although these events have been well modelled, fundamental questions remain regarding the nature and tectonic structure of the crust. Today, the Natal Valley region represents a frontier for geophysical exploration. Early attempts at imaging the crust beneath the seafloor within the south-west Indian Ocean made use of now primitive seismic reflection data acquisition and processing techniques available to them at the time (1960’s - 1970’s). Considering the advancements made in marine seismic reflection imaging, these vintage datasets lacked the horizontal and vertical resolution necessary to most accurately account for and describe features of the upper crust underlying the northern Natal Valley. This study is based on the acquisition, processing and preliminary interpretation of a newly acquired, regional seismic reflection profile, Profile Mz5-007, acquired across the northernmost region of the Natal Valley during the PAMELA-Moz5 cruise in 2016. The methods of marine seismic reflection data acquisition, processing and interpretation are described. The seismic reflection data presented in this dissertation provides a regional-scaled appraisal of the upper crust underlying the northern Natal Valley for the first time. The imagery allows a comprehensive description of crustal structures and features from the very northern Natal Valley, close to the Mozambique coastline, southwards into the southern Natal Valley offshore of Durban. The findings presented here support the notion that the crust underlying the northern Natal Valley experienced roughly N-S orientated trans-tensional stresses in relation to the breakup of West Gondwana. At least two major phases of volcanism are imaged within the seismic stratigraphy. The lower-most event underlies the Limpopo Fan in the northernmost regions of the Natal Valley at a depth of 2 seconds TWT and is interpreted as a seismic expression of the upper surface of the Lower – Middle Jurassic Karoo LIP. The progressive disappearance of this horizon towards the south coincides with the emergence of a second, overlying, as yet unreported volcanic horizon, which extends southwards throughout the Natal Valley. On the basis of its regional distribution, spatial relationship with the underlying event boundary and its deformation history, this volcanic horizon is interpreted to represent a series of basaltic lava outpourings during the Lower Cretaceous, initiated in response to trans-tensional crustal deformation associated with the breakup of West Gondwana. In addition to these two major phases of flood basalt outpouring, a number of localized volcanic flows are imaged throughout the stratigraphy, most notably over the Central Terrace. In addition, at least four major unconformities are identified, all of which are well developed within the Limpopo Fan. One of these is interpreted to represent the well-developed Mid-Cretaceous hiatus in sedimentation, providing a relative seismo-stratigraphic constraint to overlying and underlying seismic units. A review of the structural elements of the crust reveals how the northern Natal Valley is partitioned into two sub-basins separated by a prominent basement high, the Central Terrace, imaged across the central portions of Profile Mz5-007. To the north of the Central Terrace, structural deformation appears largely extensional, characterized by a large (~37 km wide) asymmetrical depression directly to the north of the Central Terrace and only minor structural faulting. To the south of the Central Terrace, trans-tensional stresses associated with withdrawal of the Falkland Malvinas Plateau have deformed the upper crust into a series of apparently southwards tilted crustal blocks, producing a series of deep (3.5 s TWT) half graben-like structures, within which syn-rift fill has an apparent dip towards the south. There is evidence to suggest ongoing reactivation of these fault structures. This zone of deformation is shown to bear resemblances to the faulting mapped onshore of the Kwa-Zulu Natal coastline, providing reason to consider the two regions as a continuous zone of deformation associated with the withdrawal of the Falkland-Malvinas Plateau during the breakup of West Gondwana. Furthermore, a number of post-sedimentary intrusive features are imaged, the largest of which is the Naude Ridge; a large pinnacle-like feature which has penetrated the upper crust towards the southern limit of the profile. This feature corresponds to an abrupt change in seafloor depth and upper crustal structure, suggesting that this feature may mark the transition between subsided continental crust interpreted to underlie the northern Natal Valley to the north and oceanic crust known to underlie the southern Natal Valley to the south.
- Full Text:
- Date Issued: 2018
- Authors: Moorcroft, David
- Date: 2018
- Subjects: Seismology , Seismology -- Kwa Zulu Natal -- Research Coasts -- Kwa Zulu Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/22387 , vital:29965
- Description: The Natal Valley is a ~1000 km long, elongated depression in the seafloor, located within the south-west Indian Ocean, between the coastline of south-east Africa and the offshore aseismic Mozambique Ridge. Knowledge of its present-day crustal structure is important to reconstruct the Gondwana supercontinent break-up history and the concurrent opening of the Indian Ocean, which started during the Jurassic, some ~180 – 150 million years ago. Major geological events relating to this supercontinent break-up including continental rifting, magmatism, the emplacement of extensive oceanic flood basalt as submarine plateaus, vertical processes associated with tectonic uplift, flexural subsidence of the margin and relative global sea-level change are recorded within the crust underlying the south-west Indian Ocean. Although these events have been well modelled, fundamental questions remain regarding the nature and tectonic structure of the crust. Today, the Natal Valley region represents a frontier for geophysical exploration. Early attempts at imaging the crust beneath the seafloor within the south-west Indian Ocean made use of now primitive seismic reflection data acquisition and processing techniques available to them at the time (1960’s - 1970’s). Considering the advancements made in marine seismic reflection imaging, these vintage datasets lacked the horizontal and vertical resolution necessary to most accurately account for and describe features of the upper crust underlying the northern Natal Valley. This study is based on the acquisition, processing and preliminary interpretation of a newly acquired, regional seismic reflection profile, Profile Mz5-007, acquired across the northernmost region of the Natal Valley during the PAMELA-Moz5 cruise in 2016. The methods of marine seismic reflection data acquisition, processing and interpretation are described. The seismic reflection data presented in this dissertation provides a regional-scaled appraisal of the upper crust underlying the northern Natal Valley for the first time. The imagery allows a comprehensive description of crustal structures and features from the very northern Natal Valley, close to the Mozambique coastline, southwards into the southern Natal Valley offshore of Durban. The findings presented here support the notion that the crust underlying the northern Natal Valley experienced roughly N-S orientated trans-tensional stresses in relation to the breakup of West Gondwana. At least two major phases of volcanism are imaged within the seismic stratigraphy. The lower-most event underlies the Limpopo Fan in the northernmost regions of the Natal Valley at a depth of 2 seconds TWT and is interpreted as a seismic expression of the upper surface of the Lower – Middle Jurassic Karoo LIP. The progressive disappearance of this horizon towards the south coincides with the emergence of a second, overlying, as yet unreported volcanic horizon, which extends southwards throughout the Natal Valley. On the basis of its regional distribution, spatial relationship with the underlying event boundary and its deformation history, this volcanic horizon is interpreted to represent a series of basaltic lava outpourings during the Lower Cretaceous, initiated in response to trans-tensional crustal deformation associated with the breakup of West Gondwana. In addition to these two major phases of flood basalt outpouring, a number of localized volcanic flows are imaged throughout the stratigraphy, most notably over the Central Terrace. In addition, at least four major unconformities are identified, all of which are well developed within the Limpopo Fan. One of these is interpreted to represent the well-developed Mid-Cretaceous hiatus in sedimentation, providing a relative seismo-stratigraphic constraint to overlying and underlying seismic units. A review of the structural elements of the crust reveals how the northern Natal Valley is partitioned into two sub-basins separated by a prominent basement high, the Central Terrace, imaged across the central portions of Profile Mz5-007. To the north of the Central Terrace, structural deformation appears largely extensional, characterized by a large (~37 km wide) asymmetrical depression directly to the north of the Central Terrace and only minor structural faulting. To the south of the Central Terrace, trans-tensional stresses associated with withdrawal of the Falkland Malvinas Plateau have deformed the upper crust into a series of apparently southwards tilted crustal blocks, producing a series of deep (3.5 s TWT) half graben-like structures, within which syn-rift fill has an apparent dip towards the south. There is evidence to suggest ongoing reactivation of these fault structures. This zone of deformation is shown to bear resemblances to the faulting mapped onshore of the Kwa-Zulu Natal coastline, providing reason to consider the two regions as a continuous zone of deformation associated with the withdrawal of the Falkland-Malvinas Plateau during the breakup of West Gondwana. Furthermore, a number of post-sedimentary intrusive features are imaged, the largest of which is the Naude Ridge; a large pinnacle-like feature which has penetrated the upper crust towards the southern limit of the profile. This feature corresponds to an abrupt change in seafloor depth and upper crustal structure, suggesting that this feature may mark the transition between subsided continental crust interpreted to underlie the northern Natal Valley to the north and oceanic crust known to underlie the southern Natal Valley to the south.
- Full Text:
- Date Issued: 2018