A social realist account of constraints and enablements navigated by South African students during the four year professional accounting programme at Rhodes University, South Africa
- Authors: Myers, Lyndrianne Peta
- Date: 2019
- Subjects: Rhodes University -- Students , Accounting -- Study and teaching (Higher) -- South Africa , Education, Higher -- South Africa , Education, Higher -- Social aspects -- South Africa , Education, Higher -- Finance -- South Africa , Higher education and state -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/93800 , vital:30946
- Description: This dissertation is an analysis of the enablements and constraints navigated by 43 students from different academic years within the Department of Accounting, Rhodes University, in their pursuit of obtaining the postgraduate Diploma in Accounting (DipAcc) qualification. Passing this diploma year entitles students to become Trainee Accountants, which is one of the requirements for their ultimate goal of becoming a chartered accountant. In the course of semi-structured, face-to-face interviews conducted for this study, students from across the four years of the professional degree programme, shared what had helped or hindered them on their journeys to and through Rhodes University, and within the Department of Accounting at this university. Focus group discussions were then held with academics from the department, where the student participants’ experiences were shared. The responses of the members of the focus groups confirmed many of the student participants’ experiences as did interviews with representatives from the South African Institute of Chartered Accountants (SAICA). These representatives also spoke about students’ experiences at other campuses. To determine how localised the student participants’ experiences were, selected individuals from a number of other Departments or Schools of Accounting at SAICA-accredited institutions in South Africa were also interviewed. SAICA representatives also discussed the ‘pervasive skills’ which trainee accountants are expected to acquire. The perspectives from these different groups, have provided validation of the student research participants’ experiences. Critical Realism and Social Realism were used as theoretical underpinnings while Social Realism, Bernstein’s Pedagogic Device, Legitimation Code Theory and New Literacies Theory were used as explanatory theories. Using these theories, the participants’ experiences were analysed and could be understood in a different way. This dissertation reveals how this unequal privileging of individuals as a result of the existing structures is perpetuated at university level. It is the poorer students from under-resourced schools who generally struggle with the language and the practices and ways of being required for success at university. Student participants’ experiences of constraint and enablement arose primarily in the areas of the finances required for tuition and living expenses while at university; having English as a language of learning; and difficulties experienced with taking advantage of the learning opportunities within the department. Research participants also spoke about their experiences of transformation in terms of both student protests, and a mentoring programme which assisted them in gaining access to the practises and ways of being required for the discipline. In so doing they were inducted into the discipline’s community of practice. This dissertation has assisted in providing an understanding of what has helped and what has hindered students at Rhodes University, on their journeys towards obtaining the Postgraduate Diploma in Accounting qualification. It has also provided insight into the mechanisms which lie behind these experiences. This study will provide practitioners and policy-makers with the opportunity to be better informed about students’ struggles, to contemplate their interactions with students and to identify, remove or reduce unnecessarily burdensome hurdles. Equally and perhaps more importantly, this study and the work which emerges as a result of this research, will provide students with tools to assist them in their academic journeys, to manage essential hurdles, and to eliminate or avoid unnecessary hurdles.
- Full Text:
- Date Issued: 2019
- Authors: Myers, Lyndrianne Peta
- Date: 2019
- Subjects: Rhodes University -- Students , Accounting -- Study and teaching (Higher) -- South Africa , Education, Higher -- South Africa , Education, Higher -- Social aspects -- South Africa , Education, Higher -- Finance -- South Africa , Higher education and state -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/93800 , vital:30946
- Description: This dissertation is an analysis of the enablements and constraints navigated by 43 students from different academic years within the Department of Accounting, Rhodes University, in their pursuit of obtaining the postgraduate Diploma in Accounting (DipAcc) qualification. Passing this diploma year entitles students to become Trainee Accountants, which is one of the requirements for their ultimate goal of becoming a chartered accountant. In the course of semi-structured, face-to-face interviews conducted for this study, students from across the four years of the professional degree programme, shared what had helped or hindered them on their journeys to and through Rhodes University, and within the Department of Accounting at this university. Focus group discussions were then held with academics from the department, where the student participants’ experiences were shared. The responses of the members of the focus groups confirmed many of the student participants’ experiences as did interviews with representatives from the South African Institute of Chartered Accountants (SAICA). These representatives also spoke about students’ experiences at other campuses. To determine how localised the student participants’ experiences were, selected individuals from a number of other Departments or Schools of Accounting at SAICA-accredited institutions in South Africa were also interviewed. SAICA representatives also discussed the ‘pervasive skills’ which trainee accountants are expected to acquire. The perspectives from these different groups, have provided validation of the student research participants’ experiences. Critical Realism and Social Realism were used as theoretical underpinnings while Social Realism, Bernstein’s Pedagogic Device, Legitimation Code Theory and New Literacies Theory were used as explanatory theories. Using these theories, the participants’ experiences were analysed and could be understood in a different way. This dissertation reveals how this unequal privileging of individuals as a result of the existing structures is perpetuated at university level. It is the poorer students from under-resourced schools who generally struggle with the language and the practices and ways of being required for success at university. Student participants’ experiences of constraint and enablement arose primarily in the areas of the finances required for tuition and living expenses while at university; having English as a language of learning; and difficulties experienced with taking advantage of the learning opportunities within the department. Research participants also spoke about their experiences of transformation in terms of both student protests, and a mentoring programme which assisted them in gaining access to the practises and ways of being required for the discipline. In so doing they were inducted into the discipline’s community of practice. This dissertation has assisted in providing an understanding of what has helped and what has hindered students at Rhodes University, on their journeys towards obtaining the Postgraduate Diploma in Accounting qualification. It has also provided insight into the mechanisms which lie behind these experiences. This study will provide practitioners and policy-makers with the opportunity to be better informed about students’ struggles, to contemplate their interactions with students and to identify, remove or reduce unnecessarily burdensome hurdles. Equally and perhaps more importantly, this study and the work which emerges as a result of this research, will provide students with tools to assist them in their academic journeys, to manage essential hurdles, and to eliminate or avoid unnecessary hurdles.
- Full Text:
- Date Issued: 2019
Post-apartheid nostalgia and the future of the black visual archive
- Nsele, Zamansele Nsikakazi Busisiwe
- Authors: Nsele, Zamansele Nsikakazi Busisiwe
- Date: 2020
- Subjects: Nostalgia in art , Memory in art , Africa -- In art , Africans in art , Fanon, Frantz, 1925-1961. Peau noire, masques blancs. English , South Africa -- In art , Black people in art
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167177 , vital:41444
- Description: The implications of nostalgia often strike a discordant note in post-apartheid discourse and this has opened up critical possibilities for research scholarship. For instance, Jacob Dlamini’s memoir Native Nostalgia entered the discursive fray in 2009, and it was subsequently followed by Derek Hook’s psychoanalytical approach in (Post) apartheid Conditions: Psychoanalysis and Social Formation in 2014. Notably, there is not yet a sustained and intensive research focus that has been conducted on post-apartheid forms of nostalgia within the discipline of art history and visual culture. I present this dissertation as a response to this gap. This thesis identifies mainly two competing nostalgias in post-apartheid South Africa. Through the analysis of selected artwork and media imagery, this dissertation critiques the connections of these nostalgias to the representation of the black figure in post-apartheid visual culture and the implications thereof. I argue that nostalgias for an apartheid-colonial-imperialist past operate through erasure and in the sanitisation of memory and as a result they render suffering indiscernible or in a sadomasochistic way consumes suffering as enjoyable. This thesis simultaneously critiques art work and visual representation that responds to South Africa’s nostalgia for the future: a restorative nostalgia that has emerged in the form of “rainbow nationalism”. This is a form of nostalgia that is underpinned by a dogged commitment to triumphalism and as a result erases the ongoing scenes of abjection. I use nostalgia and Afropessimism as analytical frameworks to argue that both real and visual representational forces work in tandem to restrain the future and this, I suggest is fulfilled by the transference of the black body from one state of unfreedom to next, resonating with a cyclical pattern. Frantz Fanon’s (1967) Black Skin White Mask forms the conceptual bedrock of my study, particularly his visual layout of “negrophobogenesis” and colonial temporality, which he describes as a “hellish cycle” or as an “infernal cycle” wherein the past overwhelms the present and ideas of the future.
- Full Text:
- Date Issued: 2020
- Authors: Nsele, Zamansele Nsikakazi Busisiwe
- Date: 2020
- Subjects: Nostalgia in art , Memory in art , Africa -- In art , Africans in art , Fanon, Frantz, 1925-1961. Peau noire, masques blancs. English , South Africa -- In art , Black people in art
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167177 , vital:41444
- Description: The implications of nostalgia often strike a discordant note in post-apartheid discourse and this has opened up critical possibilities for research scholarship. For instance, Jacob Dlamini’s memoir Native Nostalgia entered the discursive fray in 2009, and it was subsequently followed by Derek Hook’s psychoanalytical approach in (Post) apartheid Conditions: Psychoanalysis and Social Formation in 2014. Notably, there is not yet a sustained and intensive research focus that has been conducted on post-apartheid forms of nostalgia within the discipline of art history and visual culture. I present this dissertation as a response to this gap. This thesis identifies mainly two competing nostalgias in post-apartheid South Africa. Through the analysis of selected artwork and media imagery, this dissertation critiques the connections of these nostalgias to the representation of the black figure in post-apartheid visual culture and the implications thereof. I argue that nostalgias for an apartheid-colonial-imperialist past operate through erasure and in the sanitisation of memory and as a result they render suffering indiscernible or in a sadomasochistic way consumes suffering as enjoyable. This thesis simultaneously critiques art work and visual representation that responds to South Africa’s nostalgia for the future: a restorative nostalgia that has emerged in the form of “rainbow nationalism”. This is a form of nostalgia that is underpinned by a dogged commitment to triumphalism and as a result erases the ongoing scenes of abjection. I use nostalgia and Afropessimism as analytical frameworks to argue that both real and visual representational forces work in tandem to restrain the future and this, I suggest is fulfilled by the transference of the black body from one state of unfreedom to next, resonating with a cyclical pattern. Frantz Fanon’s (1967) Black Skin White Mask forms the conceptual bedrock of my study, particularly his visual layout of “negrophobogenesis” and colonial temporality, which he describes as a “hellish cycle” or as an “infernal cycle” wherein the past overwhelms the present and ideas of the future.
- Full Text:
- Date Issued: 2020
Aspects of the population ecology, habitat use and behaviour of the endangered Knysna seahorse (Hippocampus capensis Boulenger, 1900) in a residential marina estate, Knysna, South Africa: implications for conservation
- Authors: Claassens, Louw
- Date: 2018
- Subjects: Endangered species -- South Africa -- Knysna , Sea horses -- Behavior -- South Africa -- Knysna , Sea horses -- Habitat -- South Africa -- Knysna , Sea horses -- Ecology -- South Africa -- Knysna
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54789 , vital:26616
- Description: The Knysna seahorse Hippocampus capensis is South Africa’s only endemic seahorse species, and is found in only three adjacent estuaries along the southern coast. The conservation of this endangered species is important on a national and international level. This study presents the first research on this species within the Knysna estuary since 2001 and specifically focuses on aspects of its ecology within a residential marina estate (Thesen Islands Marina). The physico-chemical and habitat features of the marina were described and the population ecology, habitat use, and behaviour of the Knysna seahorse were investigated. Physico-chemical conditions within the western section of the marina, characterised by high water current velocities, were similar to that of the adjacent estuary. The eastern section of the marina was characterised by lower water current velocities and higher turbidity. Four major habitat types were identified within the marina canals: (I) artificial Reno mattress (wire baskets filled with rocks); (II) Codium tenue beds; (III) mixed vegetation on sediment; and (IV) barren canal floor. Seahorse densities within the marina were significantly higher compared to densities found historically within the estuary. Highest seahorse densities were specifically found within the artificial Reno mattress structures and within the western section of the marina. Seahorse density varied spatially and temporally and the type of habitat was an important predictor for seahorse occurrence. An experimental investigation found that H. capensis chooses artificial Reno mattress habitat over Zostera capensis when given a choice. GoPro cameras were used successfully to investigate daytime seahorse behaviour within the Reno mattress habitat. Seahorses were more active during the morning, spent most of their time (> 80 %) feeding, and morning courting behaviour for this species were confirmed. However, during the summer holiday period (mid-December to mid-January) few seahorses were observed on camera, which suggests that the increase in motor boat activity and the related increase in noise had a negative effect on H. capensis feeding and courting behaviour. The marina development, and in particular the Reno mattresses, created a new habitat for this endangered species within the Knysna estuary. In addition to the protection and restoration of natural habitats in which H. capensis is found, the conservation potential of artificial structures such as Reno mattresses should be realised.
- Full Text:
- Date Issued: 2018
- Authors: Claassens, Louw
- Date: 2018
- Subjects: Endangered species -- South Africa -- Knysna , Sea horses -- Behavior -- South Africa -- Knysna , Sea horses -- Habitat -- South Africa -- Knysna , Sea horses -- Ecology -- South Africa -- Knysna
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54789 , vital:26616
- Description: The Knysna seahorse Hippocampus capensis is South Africa’s only endemic seahorse species, and is found in only three adjacent estuaries along the southern coast. The conservation of this endangered species is important on a national and international level. This study presents the first research on this species within the Knysna estuary since 2001 and specifically focuses on aspects of its ecology within a residential marina estate (Thesen Islands Marina). The physico-chemical and habitat features of the marina were described and the population ecology, habitat use, and behaviour of the Knysna seahorse were investigated. Physico-chemical conditions within the western section of the marina, characterised by high water current velocities, were similar to that of the adjacent estuary. The eastern section of the marina was characterised by lower water current velocities and higher turbidity. Four major habitat types were identified within the marina canals: (I) artificial Reno mattress (wire baskets filled with rocks); (II) Codium tenue beds; (III) mixed vegetation on sediment; and (IV) barren canal floor. Seahorse densities within the marina were significantly higher compared to densities found historically within the estuary. Highest seahorse densities were specifically found within the artificial Reno mattress structures and within the western section of the marina. Seahorse density varied spatially and temporally and the type of habitat was an important predictor for seahorse occurrence. An experimental investigation found that H. capensis chooses artificial Reno mattress habitat over Zostera capensis when given a choice. GoPro cameras were used successfully to investigate daytime seahorse behaviour within the Reno mattress habitat. Seahorses were more active during the morning, spent most of their time (> 80 %) feeding, and morning courting behaviour for this species were confirmed. However, during the summer holiday period (mid-December to mid-January) few seahorses were observed on camera, which suggests that the increase in motor boat activity and the related increase in noise had a negative effect on H. capensis feeding and courting behaviour. The marina development, and in particular the Reno mattresses, created a new habitat for this endangered species within the Knysna estuary. In addition to the protection and restoration of natural habitats in which H. capensis is found, the conservation potential of artificial structures such as Reno mattresses should be realised.
- Full Text:
- Date Issued: 2018
Defended subjectivity in service-learning:a psychosocial analysis of students’ talk about service-learning in psychology
- Authors: Haselau, Tracey Laura
- Date: 2021
- Subjects: Service learning -- Case studies -- South Africa -- Makhanda , Rhodes University -- Students -- Attitudes , Psychology students -- Attitudes -- South Africa -- Makhanda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/170810 , vital:41962 , 10.21504/10962/170810
- Description: The purpose of this qualitative study was to analyse students’ talk about their service-learning experiences in psychology, in South Africa, from a psychosocial perspective. The research aims to identify dominant and subjugated discourses about service-learning in psychology, and to explore why students invest in particular discourses over others. Furthermore, the research aims to explore the intersubjective contexts that mediate students’ talk about their service-learning and their emotional investments in the discourses employed in their talk, drawing on the concept of mentalization. Eight psychology students were interviewed toward the end of their participation in a service-learning psychology honours course. Transcripts from the interviews as well as entries from students’ reflective journals were analysed using a psychosocial methodology. The key findings from this research point to the ways in which students oscillate between employing two competing sets of discourses about their service learning. At times, students drew on what I have referred to as a ‘discourse of rapture’, characterised by fascination with the ‘other’ and the maintenance of power imbalances. This discourse draws on a liberal traditional discourse of learning and a charity discourse of service-learning. In other parts of their talk, students draw on what I have called a ‘discourse of ruptura’, characterised by an inward curiosity about the outward fascination with the ‘other’. This discourse draws on constructivist accounts of service-learning. Findings suggest that students’ emotional investments in discourses of service-learning are mediated by defensive positions caused by the anxieties incurred in service-learning contexts. An important consideration to take forward from this research is the way in which anxieties in service-learning experiences may be contained (or not), and to be aware of the problematic outcomes that may arise from not containing anxieties, such as the perpetuation of prejudicial attitudes and othering. The intersectionality of ‘race’ and disability in the specific service-learning programme under investigation in this study is an important consideration in implementing careful supervision of programmes such as this one, so that students’ rapture with the ‘other’ is not compounded and reinforced by the service-learning experience.
- Full Text:
- Date Issued: 2021
- Authors: Haselau, Tracey Laura
- Date: 2021
- Subjects: Service learning -- Case studies -- South Africa -- Makhanda , Rhodes University -- Students -- Attitudes , Psychology students -- Attitudes -- South Africa -- Makhanda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/170810 , vital:41962 , 10.21504/10962/170810
- Description: The purpose of this qualitative study was to analyse students’ talk about their service-learning experiences in psychology, in South Africa, from a psychosocial perspective. The research aims to identify dominant and subjugated discourses about service-learning in psychology, and to explore why students invest in particular discourses over others. Furthermore, the research aims to explore the intersubjective contexts that mediate students’ talk about their service-learning and their emotional investments in the discourses employed in their talk, drawing on the concept of mentalization. Eight psychology students were interviewed toward the end of their participation in a service-learning psychology honours course. Transcripts from the interviews as well as entries from students’ reflective journals were analysed using a psychosocial methodology. The key findings from this research point to the ways in which students oscillate between employing two competing sets of discourses about their service learning. At times, students drew on what I have referred to as a ‘discourse of rapture’, characterised by fascination with the ‘other’ and the maintenance of power imbalances. This discourse draws on a liberal traditional discourse of learning and a charity discourse of service-learning. In other parts of their talk, students draw on what I have called a ‘discourse of ruptura’, characterised by an inward curiosity about the outward fascination with the ‘other’. This discourse draws on constructivist accounts of service-learning. Findings suggest that students’ emotional investments in discourses of service-learning are mediated by defensive positions caused by the anxieties incurred in service-learning contexts. An important consideration to take forward from this research is the way in which anxieties in service-learning experiences may be contained (or not), and to be aware of the problematic outcomes that may arise from not containing anxieties, such as the perpetuation of prejudicial attitudes and othering. The intersectionality of ‘race’ and disability in the specific service-learning programme under investigation in this study is an important consideration in implementing careful supervision of programmes such as this one, so that students’ rapture with the ‘other’ is not compounded and reinforced by the service-learning experience.
- Full Text:
- Date Issued: 2021
The isolation and genetic characterisation of a novel alphabaculovirus for the microbial control of Cryptophlebia peltastica and closely related tortricid pests
- Authors: Marsberg, Tamryn
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59292 , vital:27543
- Description: Cryptophlebia peltastica (Meyrick) (Lepidoptera: Tortricidae) is an economically damaging pest of litchis and macadamias in South Africa. Cryptophlebia peltastica causes both pre- and post-harvest damage to litchis, reducing overall yields and thus classifying the pest as a phytosanitary risk. Various control methods have been implemented against C. peltastica in an integrated pest management programme. These control methods include chemical control, cultural control and biological control. However, these methods have not yet provided satisfactory control as of yet. As a result, an alternative control option needs to be identified and implemented into the IPM programme. An alternative method of control that has proved successful in other agricultural sectors and not yet implemented in the control of C. peltastica is that of microbial control, specifically the use of baculovirus biopesticides. This study aimed to isolate and characterise a novel baculovirus from a laboratory culture of C. peltastica that could be used as a commercially available baculovirus biopesticide. In order to isolate a baculovirus a laboratory culture of C. peltastica was successfully established at Rhodes University, Grahamstown, South Africa. This is the first time a laboratory culture of C. peltastica has been established. This allowed for various biological aspects of the pest to be determined, which included: length of the life cycle, fecundity and time to oviposition, egg and larval development and percentage hatch. The results obtained from these studies found that the biology of C. peltastica was similar to that of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). Once the laboratory culture had reached high densities, larvae showing symptoms of baculovirus infection were observed. Symptomatic larvae were collected and examined for the presence of a baculovirus. An alphabaculovirus (NPV) was successfully isolated and morphologically identified using purified OBs that were sectioned and observed by transmission electron microscopy. This was then confirmed by amplifying the polyhedrin gene region using degenerate primers. A BLAST analysis found a 93% similarity to a partial polyhedrin gene sequence to be that of Epinotia granitalis (Butler) (Lepidoptera: Tortricidae). The alphabaculovirus was then genetically characterised by generating restriction profiles and sequencing the whole genome. Due to the novelty of the virus, no comparison could be made. The biological activity of the alphabaculovirus was then tested against C. peltastica and two closely related Tortricidae pests: T. leucotreta and Cydiapomonella (Linnaeous) (Lepidoptera: Tortricidae). The alphabaculovirus was highly virulent against all three species. The lethal concentrations (LC50 and LC90) for the virus against C. peltastica was 8.19 x 103 and 3.33 x 105 OBs/ml. The LC50 and LC90 for T. leucotreta was 2.29 x 103 and 9.97 x 104 OBs/ml, respectively and C. pomonella had a LC50 of 1.43 x 103 OBs/ml and LC90 1.26 x 104 OBs/ml. The virus was particularly virulent against T. leucotreta and C. pomonella as compared to C. peltastica. The biological activity of the alphabaculovirus was also tested against CpGV resistant European C. pomonella. From the results it was observed that the virus had the ability to overcome the resistance in C. pomonella and could potentially be used in the resistance management of C. pomonella. With the successful biological activity results obtained from this study, preliminary investigation were made into the mass production of the alphabaculovirus using both the in vivo and in vitro production methods. For in vivo production both the homologous host (C. peltastica) and a heterologous host (T leucotreta) were investigated. Preliminary studies focused on determining the biological activity in fifth instars of both hosts. Fifth instar LC50 and LC90 values for C. peltastica were 3.43 x 103 and 1.11 x 107 OBs/ml and for T. leucotreta the LC50 and LC90 values were 2.53 x 103 and 8.82 x 106 OBs/ml, respectively. The average yield of virus produced in each species was also determined. Cryptophlebia peltastica had the highest viral yield of 5.37 x 1010 OBs/larva and 2.93 x 1010 OBs/larva for T. leucotreta. The results obtained, from the preliminary investigation concluded that the virus could be produced in vivo in both C. peltastica and T. leucotreta, however further research is required into the mass production in both hosts. The in vitro production of the virus was also considered and the susceptibility of the virus was tested against the C. pomonella cell line, Cp14R. After infection of the Cp14R cells with budded virus collected from fifth instar C. peltastica larvae, OBs could be observed after three days. Thus, the alphabaculovirus is susceptible to the Cp14R cell line, thus has the potential to be produced in vitro and further characterised. This study is the first to report of the identification and characterisation of a novel alphabaculovirus isolated from a laboratory reared culture of C. peltastica and the potential for it to be commercially developed into a bipoesticide and used against Tortricidae pests.
- Full Text:
- Date Issued: 2017
- Authors: Marsberg, Tamryn
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59292 , vital:27543
- Description: Cryptophlebia peltastica (Meyrick) (Lepidoptera: Tortricidae) is an economically damaging pest of litchis and macadamias in South Africa. Cryptophlebia peltastica causes both pre- and post-harvest damage to litchis, reducing overall yields and thus classifying the pest as a phytosanitary risk. Various control methods have been implemented against C. peltastica in an integrated pest management programme. These control methods include chemical control, cultural control and biological control. However, these methods have not yet provided satisfactory control as of yet. As a result, an alternative control option needs to be identified and implemented into the IPM programme. An alternative method of control that has proved successful in other agricultural sectors and not yet implemented in the control of C. peltastica is that of microbial control, specifically the use of baculovirus biopesticides. This study aimed to isolate and characterise a novel baculovirus from a laboratory culture of C. peltastica that could be used as a commercially available baculovirus biopesticide. In order to isolate a baculovirus a laboratory culture of C. peltastica was successfully established at Rhodes University, Grahamstown, South Africa. This is the first time a laboratory culture of C. peltastica has been established. This allowed for various biological aspects of the pest to be determined, which included: length of the life cycle, fecundity and time to oviposition, egg and larval development and percentage hatch. The results obtained from these studies found that the biology of C. peltastica was similar to that of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). Once the laboratory culture had reached high densities, larvae showing symptoms of baculovirus infection were observed. Symptomatic larvae were collected and examined for the presence of a baculovirus. An alphabaculovirus (NPV) was successfully isolated and morphologically identified using purified OBs that were sectioned and observed by transmission electron microscopy. This was then confirmed by amplifying the polyhedrin gene region using degenerate primers. A BLAST analysis found a 93% similarity to a partial polyhedrin gene sequence to be that of Epinotia granitalis (Butler) (Lepidoptera: Tortricidae). The alphabaculovirus was then genetically characterised by generating restriction profiles and sequencing the whole genome. Due to the novelty of the virus, no comparison could be made. The biological activity of the alphabaculovirus was then tested against C. peltastica and two closely related Tortricidae pests: T. leucotreta and Cydiapomonella (Linnaeous) (Lepidoptera: Tortricidae). The alphabaculovirus was highly virulent against all three species. The lethal concentrations (LC50 and LC90) for the virus against C. peltastica was 8.19 x 103 and 3.33 x 105 OBs/ml. The LC50 and LC90 for T. leucotreta was 2.29 x 103 and 9.97 x 104 OBs/ml, respectively and C. pomonella had a LC50 of 1.43 x 103 OBs/ml and LC90 1.26 x 104 OBs/ml. The virus was particularly virulent against T. leucotreta and C. pomonella as compared to C. peltastica. The biological activity of the alphabaculovirus was also tested against CpGV resistant European C. pomonella. From the results it was observed that the virus had the ability to overcome the resistance in C. pomonella and could potentially be used in the resistance management of C. pomonella. With the successful biological activity results obtained from this study, preliminary investigation were made into the mass production of the alphabaculovirus using both the in vivo and in vitro production methods. For in vivo production both the homologous host (C. peltastica) and a heterologous host (T leucotreta) were investigated. Preliminary studies focused on determining the biological activity in fifth instars of both hosts. Fifth instar LC50 and LC90 values for C. peltastica were 3.43 x 103 and 1.11 x 107 OBs/ml and for T. leucotreta the LC50 and LC90 values were 2.53 x 103 and 8.82 x 106 OBs/ml, respectively. The average yield of virus produced in each species was also determined. Cryptophlebia peltastica had the highest viral yield of 5.37 x 1010 OBs/larva and 2.93 x 1010 OBs/larva for T. leucotreta. The results obtained, from the preliminary investigation concluded that the virus could be produced in vivo in both C. peltastica and T. leucotreta, however further research is required into the mass production in both hosts. The in vitro production of the virus was also considered and the susceptibility of the virus was tested against the C. pomonella cell line, Cp14R. After infection of the Cp14R cells with budded virus collected from fifth instar C. peltastica larvae, OBs could be observed after three days. Thus, the alphabaculovirus is susceptible to the Cp14R cell line, thus has the potential to be produced in vitro and further characterised. This study is the first to report of the identification and characterisation of a novel alphabaculovirus isolated from a laboratory reared culture of C. peltastica and the potential for it to be commercially developed into a bipoesticide and used against Tortricidae pests.
- Full Text:
- Date Issued: 2017
Urbanisation, foraging and household food security in urban South Africa
- Authors: Garekae, Hesekia
- Date: 2020
- Subjects: Food sovereignty -- South Africa , Food security -- South Africa , Urban density -- South Africa , Urban agriculture -- Law and legislation -- South Africa , Sustainable urban development -- South Africa , Urban ecology (Sociology) -- South Africa , Nutrition surveys -- South Africa -- Potchefstroom , Nutrition surveys -- South Africa -- Thabazimbi , Wild plants, Edible -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167054 , vital:41433
- Description: Over the past several decades, the world’s population has been rapidly urbanising, which has resulted in a marked shift of global population from rural to urban areas. About half of the global population now reside in urban areas and is projected to increase to two-thirds by the year 2050. However, the majority of the future urban population growth is projected to be largely concentrated in Asia and sub-Saharan Africa. Despite the economic prospects normally synonymous with urbanisation, the current speed and scale of urban transformation comes with formidable challenges to contemporary urban society in these two continents. Urbanisation may presents social, economic, health and environmental challenges in urban areas, not least being a shift of the locus of food insecurity from rural to urban areas, leading to poor dietary diversity among many urban dwellers. Similarly, urbanisation alters key ecosystem services and drives habitat alteration and fragmentation, biodiversity loss, proliferation of non-native species and changes on species diversity and richness. The aforementioned changes can result in a deterioration of living conditions and decline on the quality of life for the urban dwellers. However, on the other hand, urban green infrastructure is posed as vital tool in promoting liveable cities and enhancing livelihood resilience. Against this backdrop, this study portrays wild plants, a component of urban green infrastructure, as a key resource in promoting food security and dietary diversity, thereby promoting livelihood resilience and reduced vulnerability, especially of the urban poor. Framed under the ‘right to the city’ approach, this study examined urban foraging practices and their potential contribution to dietary diversity and how they are shaped by and respond to urbanisation in two medium-sized South African towns. The study was conducted in the towns of Potchefstroom and Thabazimbi, South Africa. An explanatory sequential mixed method design was employed for data collection. Multi-stage sampling was employed in selecting the study participants. Firstly, the study towns were stratified into four socio-economic zones: informal, reconstruction and development programme, township and affluent. These zones resemble high density (informal, reconstruction and development programme), medium density (township) and low density (affluent) areas. Then, a total of 374 households were randomly sampled across the socio-economic zones for the main survey (Chapter 3 and 5). A subset of this sample, i.e. 72 participants was considered for dietary recalls, alongside with a random sample of 65 non-foragers (Chapter 4). An inventory of plant species available in a given foraging space was conducted from a total of 136 plots spread across different urban spaces (Chapter 2). Semi-structured interviews were conducted with a total of 81 participants (sub population of the main survey) for elucidating data on urban dynamics (Chapter 6). Lastly, complementary in-depth interviews with 46 participants were conducted. The findings showed that different urban spaces were constituted by diverse vegetation, with 262 distinct plant species encountered across the spaces. The majority (60.7%) were indigenous to South Africa. Species composition and diversity varied with space type, being significantly high in protected areas as compared to the other space types. About half (53%) of the identified plant species had at least one documented use, with medicine, food and firewood being modal, in order of frequency. Species composition and diversity differed between forageable and non-forageable species, being significantly high for forageable species. Urban foraging was widespread, about 68% of the respondents reported foraging. Foraging provided food, fuel energy, medicine, and cultural affirmation, among others. Despite the high prevalence rate of foraging, the study did not find any meaningful contribution of wild foods to overall diets. Nonetheless, wild foods demonstrated a substantial contribution in diversifying diets within particular food groups consumed such as vegetables, thus emerging essential in mitigating monotonous urban diets, particularly of the urban poor. Besides, the prevalence of foraging differed significantly between and within towns, being high for Thabazimbi (54.7%) and residents in the outskirts of town (86.2%) than Potchefstroom (45.3%) and residents in the inner part of town (33.3%). Participation in foraging was primarily a function of childhood (91.8%) exposure and experience with foraging, positive perceptions (79%) towards practice, resident in the outskirts of town (86.2%) and low household income (49.2%). The findings indicated that foraging was driven by multifaceted motivations, with culture (91.8%), health (88.7%), livelihoods needs (75.0%) and leisure (73.8%) being the most common. Vacant spaces (54.7%), riparian areas (30%), and domestic gardens (17.9%) were the most preferred foraging spaces, albeit with differences within towns. Similarly, the mean frequency of access varied with foraging spaces, being slightly high for vacant spaces than riparian areas and domestic gardens. The majority of the respondents were unaware of both formal and informal regulations managing urban landscapes, with those acknowledging some awareness falling short of articulating on the specificities of the regulations. Besides, the findings showed that foraging practice was threatened by urban transformation. Foragers perceived that the practice has changed over time, in terms of spaces, participants and regulations. There was a decreasing trend on the number and size of foraging spaces as well as shifting demographic trends of foragers. Moreover, urban transformation brought about a new set of regulations used for managing certain spaces within the urban landscape. However, some regulations prohibited or restricted access and rights to certain spaces for foraging. Hence, this prompted foragers to devise adaptation strategies to cope with the said changing dynamics. Exploring new foraging sites within and outside forager’s neighbourhoods and negotiating access entry were the most frequently mentioned adaptation strategies. This study contributes to the emerging body of knowledge on urban foraging, which has being seldom understood in the Global South.
- Full Text:
- Date Issued: 2020
- Authors: Garekae, Hesekia
- Date: 2020
- Subjects: Food sovereignty -- South Africa , Food security -- South Africa , Urban density -- South Africa , Urban agriculture -- Law and legislation -- South Africa , Sustainable urban development -- South Africa , Urban ecology (Sociology) -- South Africa , Nutrition surveys -- South Africa -- Potchefstroom , Nutrition surveys -- South Africa -- Thabazimbi , Wild plants, Edible -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167054 , vital:41433
- Description: Over the past several decades, the world’s population has been rapidly urbanising, which has resulted in a marked shift of global population from rural to urban areas. About half of the global population now reside in urban areas and is projected to increase to two-thirds by the year 2050. However, the majority of the future urban population growth is projected to be largely concentrated in Asia and sub-Saharan Africa. Despite the economic prospects normally synonymous with urbanisation, the current speed and scale of urban transformation comes with formidable challenges to contemporary urban society in these two continents. Urbanisation may presents social, economic, health and environmental challenges in urban areas, not least being a shift of the locus of food insecurity from rural to urban areas, leading to poor dietary diversity among many urban dwellers. Similarly, urbanisation alters key ecosystem services and drives habitat alteration and fragmentation, biodiversity loss, proliferation of non-native species and changes on species diversity and richness. The aforementioned changes can result in a deterioration of living conditions and decline on the quality of life for the urban dwellers. However, on the other hand, urban green infrastructure is posed as vital tool in promoting liveable cities and enhancing livelihood resilience. Against this backdrop, this study portrays wild plants, a component of urban green infrastructure, as a key resource in promoting food security and dietary diversity, thereby promoting livelihood resilience and reduced vulnerability, especially of the urban poor. Framed under the ‘right to the city’ approach, this study examined urban foraging practices and their potential contribution to dietary diversity and how they are shaped by and respond to urbanisation in two medium-sized South African towns. The study was conducted in the towns of Potchefstroom and Thabazimbi, South Africa. An explanatory sequential mixed method design was employed for data collection. Multi-stage sampling was employed in selecting the study participants. Firstly, the study towns were stratified into four socio-economic zones: informal, reconstruction and development programme, township and affluent. These zones resemble high density (informal, reconstruction and development programme), medium density (township) and low density (affluent) areas. Then, a total of 374 households were randomly sampled across the socio-economic zones for the main survey (Chapter 3 and 5). A subset of this sample, i.e. 72 participants was considered for dietary recalls, alongside with a random sample of 65 non-foragers (Chapter 4). An inventory of plant species available in a given foraging space was conducted from a total of 136 plots spread across different urban spaces (Chapter 2). Semi-structured interviews were conducted with a total of 81 participants (sub population of the main survey) for elucidating data on urban dynamics (Chapter 6). Lastly, complementary in-depth interviews with 46 participants were conducted. The findings showed that different urban spaces were constituted by diverse vegetation, with 262 distinct plant species encountered across the spaces. The majority (60.7%) were indigenous to South Africa. Species composition and diversity varied with space type, being significantly high in protected areas as compared to the other space types. About half (53%) of the identified plant species had at least one documented use, with medicine, food and firewood being modal, in order of frequency. Species composition and diversity differed between forageable and non-forageable species, being significantly high for forageable species. Urban foraging was widespread, about 68% of the respondents reported foraging. Foraging provided food, fuel energy, medicine, and cultural affirmation, among others. Despite the high prevalence rate of foraging, the study did not find any meaningful contribution of wild foods to overall diets. Nonetheless, wild foods demonstrated a substantial contribution in diversifying diets within particular food groups consumed such as vegetables, thus emerging essential in mitigating monotonous urban diets, particularly of the urban poor. Besides, the prevalence of foraging differed significantly between and within towns, being high for Thabazimbi (54.7%) and residents in the outskirts of town (86.2%) than Potchefstroom (45.3%) and residents in the inner part of town (33.3%). Participation in foraging was primarily a function of childhood (91.8%) exposure and experience with foraging, positive perceptions (79%) towards practice, resident in the outskirts of town (86.2%) and low household income (49.2%). The findings indicated that foraging was driven by multifaceted motivations, with culture (91.8%), health (88.7%), livelihoods needs (75.0%) and leisure (73.8%) being the most common. Vacant spaces (54.7%), riparian areas (30%), and domestic gardens (17.9%) were the most preferred foraging spaces, albeit with differences within towns. Similarly, the mean frequency of access varied with foraging spaces, being slightly high for vacant spaces than riparian areas and domestic gardens. The majority of the respondents were unaware of both formal and informal regulations managing urban landscapes, with those acknowledging some awareness falling short of articulating on the specificities of the regulations. Besides, the findings showed that foraging practice was threatened by urban transformation. Foragers perceived that the practice has changed over time, in terms of spaces, participants and regulations. There was a decreasing trend on the number and size of foraging spaces as well as shifting demographic trends of foragers. Moreover, urban transformation brought about a new set of regulations used for managing certain spaces within the urban landscape. However, some regulations prohibited or restricted access and rights to certain spaces for foraging. Hence, this prompted foragers to devise adaptation strategies to cope with the said changing dynamics. Exploring new foraging sites within and outside forager’s neighbourhoods and negotiating access entry were the most frequently mentioned adaptation strategies. This study contributes to the emerging body of knowledge on urban foraging, which has being seldom understood in the Global South.
- Full Text:
- Date Issued: 2020
The electrocatalytic response of metallophthalocyanines when clicked to electrodes and to nanomaterials
- Authors: Mpeta, Lekhetho Simon
- Date: 2021
- Subjects: Phthalocyanines , Nanostructured materials , Electrocatalysis , Nanoparticles , Environmental chemistry , Electrodes , Organic wastes -- Purification
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/172191 , vital:42174 , 10.21504/10962/172191
- Description: Conjugates of nanomaterials and metallophthalocyanines (MPcs) have been prepared and their electrocatalytic activity studied. The prepared nanomaterials are zinc oxide and silver nanoparticles, reduced graphene oxide nanosheets and semiconductor quantum dots. The MPcs used in this work are cobalt (II) (1a), manganese(III) (1b) and iron (II) (1c) 2,9(10),16(17),23(24)- tetrakis 4-((4-ethynylbenzyl) oxy) phthalocyaninato, 2,9(10),16(17),23(24)- tetrakis(5-pentyn-oxy) cobalt (II) phthalocyaninato (2), 9(10),16(17),23(24)- tris-[4-tert-butylphenoxy)-2- (4-ethylbezyl-oxy) cobalt (II) phthalocyaninato (3), 9(10),16(17),23(24)- tris-[4-tertbutylphenoxy)-2-(pent-4yn-yloxy)] cobalt (II) phthalocyaninato (4), cobalt (II) (5a) and manganese (III) (5b) 2,9(10),16(17),23(24)- tetrakis [4-(4-(5-chloro-1H-benzo [d]imidazol-2-yl)phenoxy] phthalocyaninato and 9(10),16(17),23(24)- tris tert butyl phenoxy- 2- [4-(4-(5-chloro-1H-benzo[d]imidazole-2-yl)phenoxy] cobalt (II) phthalocyaninato (6). Some of these MPcs (1a, 3 and 4) were directly clicked on azide grafted electrode, while some (1b, 1c, 2, 5a and 5b) were clicked to azide functionalised nanomaterials and then drop-dried on the electrodes. One phthalocyanine (5b) was drop-dried on the electrode then silver nanoparticles were electrodeposited on it taking advantage of metal-N bond. Scanning electrochemical microscopy, voltammetry, chronoamperometry, electrochemical impedance spectroscopy are among electrochemical methods used to characterise modified electrodes. Transmission electron microscopy, X-ray photoelectron spectroscopy, Xray diffractometry, Raman spectroscopy and infrared spectroscopy were employed to study surface functionalities, morphology and topography of the nanomaterials and complexes. Electrocatalytic activity of the developed materials were studied towards oxidation of 2-mercaptoethanol, hydrazine and hydrogen peroxide while the reduction study was based on oxygen and hydrogen peroxide. In general, the conjugates displayed superior catalytic activity when compared to individual materials. Complex 2 alone and when conjugated to zinc oxide nanoparticles were studied for their nonlinear optical behaviour. And the same materials were explored for their hydrazine detection capability. The aim of this study was to develop sensitive, selective and affordable sensors for selected organic waste pollutants. Conjugates were found to achieve the aim of the study compared to when individual materials were employed.
- Full Text:
- Date Issued: 2021
- Authors: Mpeta, Lekhetho Simon
- Date: 2021
- Subjects: Phthalocyanines , Nanostructured materials , Electrocatalysis , Nanoparticles , Environmental chemistry , Electrodes , Organic wastes -- Purification
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/172191 , vital:42174 , 10.21504/10962/172191
- Description: Conjugates of nanomaterials and metallophthalocyanines (MPcs) have been prepared and their electrocatalytic activity studied. The prepared nanomaterials are zinc oxide and silver nanoparticles, reduced graphene oxide nanosheets and semiconductor quantum dots. The MPcs used in this work are cobalt (II) (1a), manganese(III) (1b) and iron (II) (1c) 2,9(10),16(17),23(24)- tetrakis 4-((4-ethynylbenzyl) oxy) phthalocyaninato, 2,9(10),16(17),23(24)- tetrakis(5-pentyn-oxy) cobalt (II) phthalocyaninato (2), 9(10),16(17),23(24)- tris-[4-tert-butylphenoxy)-2- (4-ethylbezyl-oxy) cobalt (II) phthalocyaninato (3), 9(10),16(17),23(24)- tris-[4-tertbutylphenoxy)-2-(pent-4yn-yloxy)] cobalt (II) phthalocyaninato (4), cobalt (II) (5a) and manganese (III) (5b) 2,9(10),16(17),23(24)- tetrakis [4-(4-(5-chloro-1H-benzo [d]imidazol-2-yl)phenoxy] phthalocyaninato and 9(10),16(17),23(24)- tris tert butyl phenoxy- 2- [4-(4-(5-chloro-1H-benzo[d]imidazole-2-yl)phenoxy] cobalt (II) phthalocyaninato (6). Some of these MPcs (1a, 3 and 4) were directly clicked on azide grafted electrode, while some (1b, 1c, 2, 5a and 5b) were clicked to azide functionalised nanomaterials and then drop-dried on the electrodes. One phthalocyanine (5b) was drop-dried on the electrode then silver nanoparticles were electrodeposited on it taking advantage of metal-N bond. Scanning electrochemical microscopy, voltammetry, chronoamperometry, electrochemical impedance spectroscopy are among electrochemical methods used to characterise modified electrodes. Transmission electron microscopy, X-ray photoelectron spectroscopy, Xray diffractometry, Raman spectroscopy and infrared spectroscopy were employed to study surface functionalities, morphology and topography of the nanomaterials and complexes. Electrocatalytic activity of the developed materials were studied towards oxidation of 2-mercaptoethanol, hydrazine and hydrogen peroxide while the reduction study was based on oxygen and hydrogen peroxide. In general, the conjugates displayed superior catalytic activity when compared to individual materials. Complex 2 alone and when conjugated to zinc oxide nanoparticles were studied for their nonlinear optical behaviour. And the same materials were explored for their hydrazine detection capability. The aim of this study was to develop sensitive, selective and affordable sensors for selected organic waste pollutants. Conjugates were found to achieve the aim of the study compared to when individual materials were employed.
- Full Text:
- Date Issued: 2021
Civil society engagement with water governance at a local government scale in South Africa
- Weaver, Matthew James Thanduxolo
- Authors: Weaver, Matthew James Thanduxolo
- Date: 2019
- Subjects: Government accountability -- South Africa , Civil Society -- South Africa , Political participation -- South Africa , Local government -- South Africa , Water supply -- Management , Water resources development -- South Africa , Water quality management -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/151316 , vital:39051
- Description: The South African state’s ideological commitment to a participatory approach to managing water resources and delivering services in a way that includes all stakeholders warrants critical analysis.Realising this ideological commitment has proved challenging, due largely to the complex historical, political, social, and environmental context of integrated water resource management (IWRM) in South Africa. The overarching aim of this study was to explore and expand the learning of civil societyparticipating in water governance processes at a local government scale. To address this aim a single, in-depth, four-year case study into civil society participation in water governance in the Makana Local Municipality in the Eastern Cape Province of South Africa was conducted between 2014 and 2018. The case study comprised two research phases. Both phases of the research were conducted using an adaptive transdisciplinary and participatory action research approach underpinned by General Complexity Theory. Located at the research-practice interface, the study sought to be transformative and advance both scientific research and societal goals. Qualitative research methods and inductive and deductive modes of inference were used to collect and analyse the data respectively. In the first phase of the study, a Communities of Practice theoretical framing was adopted to investigate the emergence, practice and learning of a civil society organisation (CSO), Water for Dignity (WfD), in response to household water service delivery issues in the municipality. This phase served to build an understanding of factors that enabled and constrained the practice of WfD in addressing local water service issues, and of their role as social learning agents in building water-related knowledge in their community. As participation with the first civil society organisation fragmented, the opportunity arose for local government, the National Department of Water and Sanitation and civil society to co-engage. This opened up the second phase of the research during which the role of a multi-stakeholder platform, the Makana Water Forum (MWF), in enabling democratic water governance was investigated. The MWF was South Africa’s first catchment management forum with an integrated water service and water resource management agenda. In this phase, the study drew on interventionist methodology, Change Laboratory, from Cultural Historical Activity Theory (CHAT) to 1) describe the historical development, composition and shared purpose of the MWF multi-activity system constellation; and to 2) guide participants through seven learning actions (expansive learning cycle) to identify, analyse, model and implement remedial actions to problematic aspects (contradictions) of the MWF practice. Participants of the Change Laboratory workshops built their individual and collective transformative agency (deliberate actions to transform a problematic situation) as they navigated the expansive learning process. The development of this agency was identified through a micro-analysis of agentive talk. The two phases served to advance the exploration of civil society participation from informal participation to address water issues, to formal participation in water governance processes at a local government scale. Findings from the first phase of the research revealed that dedicated and sustained support of partners with distributed expertise and the highly motivated core members of WfD enabled the practice and emergence of the civil society organisation. However, factors such as poor internal leadership, power dynamics between supporting partners, socio-economic constraints and a deeply embedded lack of agency were found to be key constraining factors to WfD’s practice. Findings revealed that learning at WfD team level occurred in four ways, through learning as belonging, learning as doing, learning as experiencing and learning as becoming. The WfD CSO was able to catalyse social learning related to personal water rights; and best practices for improving water quality and water conservation in their wider community of practice. Social learning was fostered mainly through structured citizen engagements offered by WfD. The research provides evidence that civil society organisations can play an important role in bridging water-related knowledge gaps and can foster active citizenship in South African communities. However, despite significant inputs of support and resources through the engaged transdisciplinary research process, the practice and learning outcomes of WfD had a marginal transformative impact on improving the citizen’s every-day water service experiences. Findings from the second phase of the study revealed the MWF to resemble a multi-activity system constellation with a multiple, partially overlapping. interests related to the management of water. The establishment, function and contradictions constraining the function of the MWF were influenced by past cycles of participatory water governance-related activity and practice at national and local scales. Through the Change Laboratory process, 25 contradictions were identified that appeared to constrain the ability of the MWF to enable inclusive and meaningful participatory water governance. Through a process of expansive learning, participants sought to overcome three sets of contradictions grouped as Problem Themes: lack of clear focus of the MWF; representation, representivity and nonattendance; and the MWF relationship with the Makana Local Municipality. Remedial actions modelled and those enacted have improved aspects of the function of the MWF (such as diversified modes of engagement and a more focussed agenda) but have only resulted in incremental shifts towards enabling improved participatory practice, most markedly in building a collaborative relationship and trust between the MWF members, municipal and government officials. The microanalysis of agentive talk revealed seven different expressions of transformative agency. Constraining socio-economic and political conditions and the limited capacity of the coordinating committee of the MWF hindered the development of transformative agency to the extent to which concrete actions were implemented. Findings further revealed that contextualising a Change Laboratory process within the adaptive cycle of a complex social-ecological system, and the particular opportunity context the system presents, could inform the enactment of agency and its potential impact on the transformation of the system. The intervention with the MWF was too short a process to clearly observe the effects of transformative actions on the sustainability of the Makana Local Municipality water system. However, long-chain transformative agency through the development of one-on-one engagements, learning journeys and a reflexive component to the MWF engagements could support transformative pathways to sustainability in the municipality and water management system. The study contributes in-depth insight into the key role of learning as a catalyst in transformative processes. Learning improves the collaborative and adaptive capacity of people, and therefore, water management institutions, to manage explicitly for the complexity inherent in “complex” socialecological systems. It provides empirical evidence as to what enables and constrains “real” participation and learning in grass-roots water governance processes in the context of a shifting national drive towards a more adaptive and developmental Integrated Water Resource Management approach. It further provides methodological contributions to 1) the application of the Change Laboratory method with multi-activity system constellations in developing world contexts and 2) value and limitations of extended and engaged transdisciplinary research. Lastly, it provides practical recommendations to the establishment and sustainable function of both community-based CSOs and multi-stakeholder platforms engaging in water governance processes.
- Full Text:
- Date Issued: 2019
- Authors: Weaver, Matthew James Thanduxolo
- Date: 2019
- Subjects: Government accountability -- South Africa , Civil Society -- South Africa , Political participation -- South Africa , Local government -- South Africa , Water supply -- Management , Water resources development -- South Africa , Water quality management -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/151316 , vital:39051
- Description: The South African state’s ideological commitment to a participatory approach to managing water resources and delivering services in a way that includes all stakeholders warrants critical analysis.Realising this ideological commitment has proved challenging, due largely to the complex historical, political, social, and environmental context of integrated water resource management (IWRM) in South Africa. The overarching aim of this study was to explore and expand the learning of civil societyparticipating in water governance processes at a local government scale. To address this aim a single, in-depth, four-year case study into civil society participation in water governance in the Makana Local Municipality in the Eastern Cape Province of South Africa was conducted between 2014 and 2018. The case study comprised two research phases. Both phases of the research were conducted using an adaptive transdisciplinary and participatory action research approach underpinned by General Complexity Theory. Located at the research-practice interface, the study sought to be transformative and advance both scientific research and societal goals. Qualitative research methods and inductive and deductive modes of inference were used to collect and analyse the data respectively. In the first phase of the study, a Communities of Practice theoretical framing was adopted to investigate the emergence, practice and learning of a civil society organisation (CSO), Water for Dignity (WfD), in response to household water service delivery issues in the municipality. This phase served to build an understanding of factors that enabled and constrained the practice of WfD in addressing local water service issues, and of their role as social learning agents in building water-related knowledge in their community. As participation with the first civil society organisation fragmented, the opportunity arose for local government, the National Department of Water and Sanitation and civil society to co-engage. This opened up the second phase of the research during which the role of a multi-stakeholder platform, the Makana Water Forum (MWF), in enabling democratic water governance was investigated. The MWF was South Africa’s first catchment management forum with an integrated water service and water resource management agenda. In this phase, the study drew on interventionist methodology, Change Laboratory, from Cultural Historical Activity Theory (CHAT) to 1) describe the historical development, composition and shared purpose of the MWF multi-activity system constellation; and to 2) guide participants through seven learning actions (expansive learning cycle) to identify, analyse, model and implement remedial actions to problematic aspects (contradictions) of the MWF practice. Participants of the Change Laboratory workshops built their individual and collective transformative agency (deliberate actions to transform a problematic situation) as they navigated the expansive learning process. The development of this agency was identified through a micro-analysis of agentive talk. The two phases served to advance the exploration of civil society participation from informal participation to address water issues, to formal participation in water governance processes at a local government scale. Findings from the first phase of the research revealed that dedicated and sustained support of partners with distributed expertise and the highly motivated core members of WfD enabled the practice and emergence of the civil society organisation. However, factors such as poor internal leadership, power dynamics between supporting partners, socio-economic constraints and a deeply embedded lack of agency were found to be key constraining factors to WfD’s practice. Findings revealed that learning at WfD team level occurred in four ways, through learning as belonging, learning as doing, learning as experiencing and learning as becoming. The WfD CSO was able to catalyse social learning related to personal water rights; and best practices for improving water quality and water conservation in their wider community of practice. Social learning was fostered mainly through structured citizen engagements offered by WfD. The research provides evidence that civil society organisations can play an important role in bridging water-related knowledge gaps and can foster active citizenship in South African communities. However, despite significant inputs of support and resources through the engaged transdisciplinary research process, the practice and learning outcomes of WfD had a marginal transformative impact on improving the citizen’s every-day water service experiences. Findings from the second phase of the study revealed the MWF to resemble a multi-activity system constellation with a multiple, partially overlapping. interests related to the management of water. The establishment, function and contradictions constraining the function of the MWF were influenced by past cycles of participatory water governance-related activity and practice at national and local scales. Through the Change Laboratory process, 25 contradictions were identified that appeared to constrain the ability of the MWF to enable inclusive and meaningful participatory water governance. Through a process of expansive learning, participants sought to overcome three sets of contradictions grouped as Problem Themes: lack of clear focus of the MWF; representation, representivity and nonattendance; and the MWF relationship with the Makana Local Municipality. Remedial actions modelled and those enacted have improved aspects of the function of the MWF (such as diversified modes of engagement and a more focussed agenda) but have only resulted in incremental shifts towards enabling improved participatory practice, most markedly in building a collaborative relationship and trust between the MWF members, municipal and government officials. The microanalysis of agentive talk revealed seven different expressions of transformative agency. Constraining socio-economic and political conditions and the limited capacity of the coordinating committee of the MWF hindered the development of transformative agency to the extent to which concrete actions were implemented. Findings further revealed that contextualising a Change Laboratory process within the adaptive cycle of a complex social-ecological system, and the particular opportunity context the system presents, could inform the enactment of agency and its potential impact on the transformation of the system. The intervention with the MWF was too short a process to clearly observe the effects of transformative actions on the sustainability of the Makana Local Municipality water system. However, long-chain transformative agency through the development of one-on-one engagements, learning journeys and a reflexive component to the MWF engagements could support transformative pathways to sustainability in the municipality and water management system. The study contributes in-depth insight into the key role of learning as a catalyst in transformative processes. Learning improves the collaborative and adaptive capacity of people, and therefore, water management institutions, to manage explicitly for the complexity inherent in “complex” socialecological systems. It provides empirical evidence as to what enables and constrains “real” participation and learning in grass-roots water governance processes in the context of a shifting national drive towards a more adaptive and developmental Integrated Water Resource Management approach. It further provides methodological contributions to 1) the application of the Change Laboratory method with multi-activity system constellations in developing world contexts and 2) value and limitations of extended and engaged transdisciplinary research. Lastly, it provides practical recommendations to the establishment and sustainable function of both community-based CSOs and multi-stakeholder platforms engaging in water governance processes.
- Full Text:
- Date Issued: 2019
Making South African tertiary education multilingual: the implementation, monitoring and evaluation of SANTED Multilingualism Projects 2007 – 2010
- Authors: Nosilela, Bulelwa
- Date: 2020
- Subjects: South Africa-Norway Tertiary Education Development Programme , Language policy -- South Africa , Multilingual education -- South Africa , Language and education -- South Africa , Education, Higher -- Social aspects -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10962/140513 , vital:37895
- Description: This thesis focuses on multilingualism as it applies to education, particularly higher education in South Africa. The thesis suggests that language must be seen as critical in education (Obanya, 2004; Wolff, 2006). This thesis also suggests that there is a link between a person’s general competence in an educational environment, and that person’s language competence. In support of other scholars, this thesis also finds that learners from low social classes in society have a restricted linguistic code and therefore the learners from these social classes lack the ability to express themselves in an elaborative manner in a cognitive situation while learners from upper social class have an elaborate linguistic repertoire in the language of instruction, namely English. Even though general policy in South Africa and Africa as a whole recognises this relationship between language and education, this thesis finds that very little is being done to implement these well-meaning policies. The SANTED (South Africa-Norway-Tertiary-Education) programme, which forms the focus of this thesis, is seen as an exception. While there is an increase in the number of learners and students accessing centres of learning, especially those from historically-disadvantaged backgrounds (CHE Higher Education Monitor, 2008), there is at the same time rigorous debate in South Africa at the moment as to whether these learners and students benefit optimally from knowledge offered in centres of learning, and are therefore, not accomplishing success expected from them (Boughey, 2008). This thesis suggests that at the centre of this debate is the understanding that, while there are a variety of factors that might affect their success, for example social and economic status, schooling background in case of students in higher education, language plays a pivotal role. This thesis analyses the challenges and successes which faced the SANTED programme at a number of South African institutions where language issues were brought to the centre of the learning process. The thesis also assesses best practices in various African countries and makes recommendations regarding language-use in Higher Education, as part of a transformationprocess. The collaboration between the South African and Norwegian governments provided impetus to policy implementation. It also had other focus areas such as capacity building, access, success and retention and collaboration with Southern African Developing Countries (SADC). This thesis shows, through a critical analysis of SANTED, that it represented an important intervention regarding the promotion of multilingualism.
- Full Text:
- Date Issued: 2020
- Authors: Nosilela, Bulelwa
- Date: 2020
- Subjects: South Africa-Norway Tertiary Education Development Programme , Language policy -- South Africa , Multilingual education -- South Africa , Language and education -- South Africa , Education, Higher -- Social aspects -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10962/140513 , vital:37895
- Description: This thesis focuses on multilingualism as it applies to education, particularly higher education in South Africa. The thesis suggests that language must be seen as critical in education (Obanya, 2004; Wolff, 2006). This thesis also suggests that there is a link between a person’s general competence in an educational environment, and that person’s language competence. In support of other scholars, this thesis also finds that learners from low social classes in society have a restricted linguistic code and therefore the learners from these social classes lack the ability to express themselves in an elaborative manner in a cognitive situation while learners from upper social class have an elaborate linguistic repertoire in the language of instruction, namely English. Even though general policy in South Africa and Africa as a whole recognises this relationship between language and education, this thesis finds that very little is being done to implement these well-meaning policies. The SANTED (South Africa-Norway-Tertiary-Education) programme, which forms the focus of this thesis, is seen as an exception. While there is an increase in the number of learners and students accessing centres of learning, especially those from historically-disadvantaged backgrounds (CHE Higher Education Monitor, 2008), there is at the same time rigorous debate in South Africa at the moment as to whether these learners and students benefit optimally from knowledge offered in centres of learning, and are therefore, not accomplishing success expected from them (Boughey, 2008). This thesis suggests that at the centre of this debate is the understanding that, while there are a variety of factors that might affect their success, for example social and economic status, schooling background in case of students in higher education, language plays a pivotal role. This thesis analyses the challenges and successes which faced the SANTED programme at a number of South African institutions where language issues were brought to the centre of the learning process. The thesis also assesses best practices in various African countries and makes recommendations regarding language-use in Higher Education, as part of a transformationprocess. The collaboration between the South African and Norwegian governments provided impetus to policy implementation. It also had other focus areas such as capacity building, access, success and retention and collaboration with Southern African Developing Countries (SADC). This thesis shows, through a critical analysis of SANTED, that it represented an important intervention regarding the promotion of multilingualism.
- Full Text:
- Date Issued: 2020
La masculinité dans quelques oeuvres des romanciers Francophones Africains
- Joseph, Joy Ifeanyichukwu Osarumwense
- Authors: Joseph, Joy Ifeanyichukwu Osarumwense
- Date: 2019
- Subjects: Masculinity in literature , Men in literature , African fiction (French) -- History and criticism , Bâ, Mariama. Chant écarlate , Fassinou, Adélaïde, 1955-.Modukpè le rêve brisé , Sanusi, Ramonu Abiodun, 1961-.Le bistouri des larmes , Sanusi, Ramonu Abiodun, 1961-.Un nègre a violé une blonde à Dallas , Baboni, Azaratou.Vie de femme, vie de sang
- Language: French
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/97512 , vital:31446 , DOI 10.21504/10962/97512
- Description: This thesis is a sociocritic and comparative study in Francophone African Literature which examines how male and female authors depict masculinity amongst African men in selected works of Francophone authors and how it affects the emancipation of women in the society. These include Mariama Bâ’s Un chant écarlate (1981) from Senegal, Adelaïde Fassinou’s Modukpè le rêve brisé (2000) from Benin, Ramonu Sanusi’s Le bistouri des larmes (2005, 2010) from Nigeria, Azaratou Baboni’s Vie de femme, vie de sang, (2011) from Benin, Ramonu Sanusi’s Un nègre a violé une blonde à Dallas, (2016) from Nigeria. The study analyses the various forms of masculinity in the selected works of Francophone authors. The study also examines the notable similarities and differences in the portrayal of male characters in the novel and how prevailing environmental factors determine the themes in the novel. This study has demonstrated that hegemonic and marginalized forms of masculinity are the dominant forms of masculinity in West Africa. These forms of masculinity have negative effects not only on the woman but affects the society in general. Furthermore, the study pleads for a social change with respect to Molara Leslie-Oguundipe’s theory on stiwanism (Ogundipe 1994: 229-230) The thesis is made of six chapters. In the first chapter, we outlined the study’s subject matter, its aims and objectives, its significance, its assumptions and methodology. In the second chapter, we examined Raewyn Connell’s theory of Masculinity and Molara Leslie-Ogundipe’s theory of Stiwanism with respect to the selected novels. Raewyn Connell’s theory on masculinity a sociological theory was employed in analyzing the social patterns of societal relationships, social interaction and culture, while Molara Leslie-Ogundipe’s theory on stiwanism a literary theory mirrored the West African Society. Such analyses helped us in identifying how socio-cultural and religious contexts influence the attitude of men towards women. In the third chapter, we applied sociocritic and comparative analyses between Mariama’s Bâ’s Un Chant Écarlate and Adelaïde’s Fassinou’s Modukpè le rêve brisé. The study focuses on how hegemonic and marginalized forms of masculinity influences racial discrimination in mixed marriages, polygamy, unwanted pregnancy, secondary sterility and single motherhood. These masculinities had negative effects on women and the society. The study also analyzed how both male and female characters employed stiwanist strategy in enhancing peace and harmony in the society. In the fourth chapter, the study examined how hegemonic and marginalized forms of masculinity influenced domestic violence, primary sterility, female genital mutilation and its negative and permanent damage to the health of women, the loss of lives of women and children in Ramonu Sanusi’s Le bistouri des larmes and Baboni Azaratou’s Vie de femme, vie de sang. The study also examined how women can rise above traditional norms with respect to stiwanist activites. The fifth chapter analyzed the influence of socio-cultural and political challenges in a contemporary society with respect to marginalized form of masculinity. In the sixth chapter, the study concluded that hegemonic and marginalized forms of masculinity are the dominant forms of masculinity. These forms of masculinites have negative effects on both sexes (male and female) as well as the society.
- Full Text:
- Date Issued: 2019
- Authors: Joseph, Joy Ifeanyichukwu Osarumwense
- Date: 2019
- Subjects: Masculinity in literature , Men in literature , African fiction (French) -- History and criticism , Bâ, Mariama. Chant écarlate , Fassinou, Adélaïde, 1955-.Modukpè le rêve brisé , Sanusi, Ramonu Abiodun, 1961-.Le bistouri des larmes , Sanusi, Ramonu Abiodun, 1961-.Un nègre a violé une blonde à Dallas , Baboni, Azaratou.Vie de femme, vie de sang
- Language: French
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/97512 , vital:31446 , DOI 10.21504/10962/97512
- Description: This thesis is a sociocritic and comparative study in Francophone African Literature which examines how male and female authors depict masculinity amongst African men in selected works of Francophone authors and how it affects the emancipation of women in the society. These include Mariama Bâ’s Un chant écarlate (1981) from Senegal, Adelaïde Fassinou’s Modukpè le rêve brisé (2000) from Benin, Ramonu Sanusi’s Le bistouri des larmes (2005, 2010) from Nigeria, Azaratou Baboni’s Vie de femme, vie de sang, (2011) from Benin, Ramonu Sanusi’s Un nègre a violé une blonde à Dallas, (2016) from Nigeria. The study analyses the various forms of masculinity in the selected works of Francophone authors. The study also examines the notable similarities and differences in the portrayal of male characters in the novel and how prevailing environmental factors determine the themes in the novel. This study has demonstrated that hegemonic and marginalized forms of masculinity are the dominant forms of masculinity in West Africa. These forms of masculinity have negative effects not only on the woman but affects the society in general. Furthermore, the study pleads for a social change with respect to Molara Leslie-Oguundipe’s theory on stiwanism (Ogundipe 1994: 229-230) The thesis is made of six chapters. In the first chapter, we outlined the study’s subject matter, its aims and objectives, its significance, its assumptions and methodology. In the second chapter, we examined Raewyn Connell’s theory of Masculinity and Molara Leslie-Ogundipe’s theory of Stiwanism with respect to the selected novels. Raewyn Connell’s theory on masculinity a sociological theory was employed in analyzing the social patterns of societal relationships, social interaction and culture, while Molara Leslie-Ogundipe’s theory on stiwanism a literary theory mirrored the West African Society. Such analyses helped us in identifying how socio-cultural and religious contexts influence the attitude of men towards women. In the third chapter, we applied sociocritic and comparative analyses between Mariama’s Bâ’s Un Chant Écarlate and Adelaïde’s Fassinou’s Modukpè le rêve brisé. The study focuses on how hegemonic and marginalized forms of masculinity influences racial discrimination in mixed marriages, polygamy, unwanted pregnancy, secondary sterility and single motherhood. These masculinities had negative effects on women and the society. The study also analyzed how both male and female characters employed stiwanist strategy in enhancing peace and harmony in the society. In the fourth chapter, the study examined how hegemonic and marginalized forms of masculinity influenced domestic violence, primary sterility, female genital mutilation and its negative and permanent damage to the health of women, the loss of lives of women and children in Ramonu Sanusi’s Le bistouri des larmes and Baboni Azaratou’s Vie de femme, vie de sang. The study also examined how women can rise above traditional norms with respect to stiwanist activites. The fifth chapter analyzed the influence of socio-cultural and political challenges in a contemporary society with respect to marginalized form of masculinity. In the sixth chapter, the study concluded that hegemonic and marginalized forms of masculinity are the dominant forms of masculinity. These forms of masculinites have negative effects on both sexes (male and female) as well as the society.
- Full Text:
- Date Issued: 2019
Mechanism of action of non-synonymous single nucleotide variations associated with α-carbonic anhydrases II, IV and VIII
- Authors: Sanyanga, T. Allan
- Date: 2020
- Subjects: Carbonic anhydrase , Carbonic anhydrase -- Therapeutic use , Nucleotides
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167346 , vital:41470
- Description: The carbonic anhydrase (CA) group of enzymes are Zinc (Zn2+) metalloproteins responsible for the reversible hydration of CO2 to bicarbonate (BCT or HCO− 3 ) and protons (H+) for the facilitation of acid-base balance and homeostasis within the body. Across all organisms, a minimum of six CA families exist, including, α (alpha), β (beta), γ (gamma), δ (delta), η (eta) and ζ (zeta). Some organisms can have more than one family, with exception to humans that contain the α family solely. The α-CA family comprises of 16 isoforms (CA-I to CA-XV) including the CA-VIII, CA-X and CA-XI acatalytic isoforms. Of the catalytic isoforms, CA-II and CA-IV possess one of the fastest rates of reaction, and any disturbances to the function of these enzymes results in CA deficiencies and undesirable phenotypes. CA-II deficiencies result in osteopetrosis with renal tubular acidosis and cerebral calcification, whereas CA-IV deficiencies result in retinitis pigmentosa 17 (RP17). Phenotypic effects generally manifest as a result of poor protein folding and function due to the presence of non-synonymous single nucleotide variations (nsSNVs). Even within the acatalytic isoforms such as CA-VIII that llosterically regulates the affinity of inositol triphosphate (IP3) for the IP3 receptor type 1 (ITPR1) and regulates calcium (Ca2+) signalling, the presence of SNVs also causes phenotypes cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CAMRQ3). Currently the majority of research into the CAs is focused on the inhibition of these proteins to achieve therapeutic effects in patients via the control of HCO− production or reabsorption as observed in glaucoma and diuretic medications. Little research has therefore been devoted into the identification of stabilising or activating compound that could rescue protein function in the case of deficiencies. The main aim of this research was to identify and characterise the effects of nsSNVs on the structure and function of CA-II, CA-IV and CA-VIII to set a foundation for rare disease studies into the CA group of proteins. Combined bioinformatics approaches divided into four main objectives were implemented. These included variant identification, sequence analysis and protein characterisation, force field (FF) parameter generation, molecular dynamics (MD) simulation and dynamic residue network analysis (DRN). Six variants for each of the CA-II, CA-IV and CA-VIII proteins with pathogenic annotations were identified from the HUMA and Ensembl databases. These included the pathogenic variants K18E, K18Q, H107Y, P236H, P236R and N252D for CA-II. CA-IV included the pathogenic R69H, R219C and R219S, and benign N86K, N177K and V234I variants. CA-VIII included pathogenic S100A, S100P, G162R and R237Q, and benign S100L and E109D variants. CA-II has been more extensively studied than CA-IV and CA-VIII, therefore residues essential to its function and stability are known. To discover important residues and regions within the CA-IV and CA-VIII proteins sequence and motif analysis was performed across the α-CA family, using CA-II as a reference. Sequence analysis identified multiple conserved residues between the two acatalytic CA-II and CA-IV, and the acatalytic CA-VIII isoforms that were proposed to be essential for protein stability. With exception to the benign N86K CA-IV variant, none of the other pathogenic or benign CA-II, CA-IV and CA-VIII SNVs were located at functionally or structurally important residues. Motif analysis identified 11 conserved and important motifs within the α-CA family. Several of the identified variants were located on these motifs including K18E, K18Q, H107Y and N252D (CA-II); N86K, R219C, R219S and V234I (CA-IV); and E109D, G162R and R237Q (CA-VIII). As there were no x-ray crystal structures of the variant proteins, homology modelling was performed to calculate the protein structures for characterisation. In CA-VIII, the substitution of Ser for Pro at position 100 (variant S100P) resulted in destruction of the β-sheet that the SNV was located on. Little is known about the mechanism of interaction between CA-VIII and ITPR1, and residues involved. SiteMap and CPORT were used to identify binding site amino for CA-VIII and results identified 38 potential residues. Traditional FFs are incapable of performing MD simulations of metalloproteins. The AMBER ff14SB FF was extended and Zn2+ FF parameters calculated to add support for metalloprotein MD simulations. In the protein, Zn2+ was noted to have a charge less than +1. Variant effects on protein structure were then investigated using MD simulations. Root mean square deviation (RMSD) and radius of gyration (Rg) results indicated subtle SNV effects to the variant global structure in CA-II and CA-IV. However, with regards to CA-VIII RMSD analysis highlighted that variant presence was associated with increases to the structural rigidity of the protein. Principal component analysis (PCA) in conjunction with free energy analysis was performed to observe variant effects on protein conformational sampling in 3D space. The binding of BCT to CA-II induced greater protein conformational sampling and was associated with higher free energy. In CA-IV and CA-VIII PCA analysis revealed key differences in the mechanism of action of pathogenic and benign SNVs. In CA-IV, wild-type (WT) and benign variant protein structures clustered into single low energy well hinting at the presence of more stable structures. Pathogenic variants were associated with higher free energy and proteins sampled more conformations without settling into a low energy well. PCA analysis of CA-VIII indicated the opposite to CA-IV. Pathogenic variants were clustered into low energy wells, while the WT and benign variants showed greater conformational sampling. Dynamic cross correlation (DCC) analysis was performed using the MD-TASK suite to determine variant effects on residue movement. CA-II WT protein revealed that BCT and CO2 were associated with anti-correlated and correlated residue movement, highlighting at opposite mechanisms. In CA-IV and CA-VIII variant presence resulted in a change to residue correlation compared to the WT proteins. DRN analysis was performed to investigate SNV effects of residue accessibility and communication. Results demonstrated that SNVs are associated with allosteric effects on the CA protein structures, and effects are located on the stability assisting residues of the aromatic clusters and the active site of the proteins. CA-II studies discovered that Glu117 is the most important residue for communication, and variant presence results in a decrease to the usage of the residue. This effect was greatest in the CA-II H107Y SNV, and suggests that variants could have an effect on Zn2+ dissociation from the active site. Decreases to the usage of Zn2+ coordinating residues were also noted. Where this occurred, compensatory increases to the usage of other primary and secondary coordination residues were observed, that could possibly assist with the maintenance of Zn2+ within the active site. The CA-IV variants R69H and R219C highlighted potentially similar pathogenic mechanisms, whereas N86K and N177K hinted at potentially similar benign mechanisms. Within CA-VIII, variant presence was associated with changes to the accessibility of the N-terminal binding site residues. The benign CA-VIII variants highlighted possible compensatory mechanisms, whereby as one group of N-terminal residues loses accessibility, there was an increase to the accessibility of other binding site residues to possibly balance the effect. Catalytically, the proton shuttle residue His64 in CA-II was found to occupy a novel conformation named the “faux in” that brought the imidazole group even closer to the Zn2+ compared to the “in” conformation. Overall, compared to traditional MD simulations the incorporation of DRN allowed more detailed investigations into the variant mechanisms of action. This highlights the importance of network analysis in the study of the effects of missense mutations on the structure and function of proteins. Investigations of diseases at the molecular level is essential in the identification of disease pathogenesis and assists with the development of specifically tailored and better treatment options especially in the cases of genetically associated rare diseases.
- Full Text:
- Date Issued: 2020
- Authors: Sanyanga, T. Allan
- Date: 2020
- Subjects: Carbonic anhydrase , Carbonic anhydrase -- Therapeutic use , Nucleotides
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167346 , vital:41470
- Description: The carbonic anhydrase (CA) group of enzymes are Zinc (Zn2+) metalloproteins responsible for the reversible hydration of CO2 to bicarbonate (BCT or HCO− 3 ) and protons (H+) for the facilitation of acid-base balance and homeostasis within the body. Across all organisms, a minimum of six CA families exist, including, α (alpha), β (beta), γ (gamma), δ (delta), η (eta) and ζ (zeta). Some organisms can have more than one family, with exception to humans that contain the α family solely. The α-CA family comprises of 16 isoforms (CA-I to CA-XV) including the CA-VIII, CA-X and CA-XI acatalytic isoforms. Of the catalytic isoforms, CA-II and CA-IV possess one of the fastest rates of reaction, and any disturbances to the function of these enzymes results in CA deficiencies and undesirable phenotypes. CA-II deficiencies result in osteopetrosis with renal tubular acidosis and cerebral calcification, whereas CA-IV deficiencies result in retinitis pigmentosa 17 (RP17). Phenotypic effects generally manifest as a result of poor protein folding and function due to the presence of non-synonymous single nucleotide variations (nsSNVs). Even within the acatalytic isoforms such as CA-VIII that llosterically regulates the affinity of inositol triphosphate (IP3) for the IP3 receptor type 1 (ITPR1) and regulates calcium (Ca2+) signalling, the presence of SNVs also causes phenotypes cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CAMRQ3). Currently the majority of research into the CAs is focused on the inhibition of these proteins to achieve therapeutic effects in patients via the control of HCO− production or reabsorption as observed in glaucoma and diuretic medications. Little research has therefore been devoted into the identification of stabilising or activating compound that could rescue protein function in the case of deficiencies. The main aim of this research was to identify and characterise the effects of nsSNVs on the structure and function of CA-II, CA-IV and CA-VIII to set a foundation for rare disease studies into the CA group of proteins. Combined bioinformatics approaches divided into four main objectives were implemented. These included variant identification, sequence analysis and protein characterisation, force field (FF) parameter generation, molecular dynamics (MD) simulation and dynamic residue network analysis (DRN). Six variants for each of the CA-II, CA-IV and CA-VIII proteins with pathogenic annotations were identified from the HUMA and Ensembl databases. These included the pathogenic variants K18E, K18Q, H107Y, P236H, P236R and N252D for CA-II. CA-IV included the pathogenic R69H, R219C and R219S, and benign N86K, N177K and V234I variants. CA-VIII included pathogenic S100A, S100P, G162R and R237Q, and benign S100L and E109D variants. CA-II has been more extensively studied than CA-IV and CA-VIII, therefore residues essential to its function and stability are known. To discover important residues and regions within the CA-IV and CA-VIII proteins sequence and motif analysis was performed across the α-CA family, using CA-II as a reference. Sequence analysis identified multiple conserved residues between the two acatalytic CA-II and CA-IV, and the acatalytic CA-VIII isoforms that were proposed to be essential for protein stability. With exception to the benign N86K CA-IV variant, none of the other pathogenic or benign CA-II, CA-IV and CA-VIII SNVs were located at functionally or structurally important residues. Motif analysis identified 11 conserved and important motifs within the α-CA family. Several of the identified variants were located on these motifs including K18E, K18Q, H107Y and N252D (CA-II); N86K, R219C, R219S and V234I (CA-IV); and E109D, G162R and R237Q (CA-VIII). As there were no x-ray crystal structures of the variant proteins, homology modelling was performed to calculate the protein structures for characterisation. In CA-VIII, the substitution of Ser for Pro at position 100 (variant S100P) resulted in destruction of the β-sheet that the SNV was located on. Little is known about the mechanism of interaction between CA-VIII and ITPR1, and residues involved. SiteMap and CPORT were used to identify binding site amino for CA-VIII and results identified 38 potential residues. Traditional FFs are incapable of performing MD simulations of metalloproteins. The AMBER ff14SB FF was extended and Zn2+ FF parameters calculated to add support for metalloprotein MD simulations. In the protein, Zn2+ was noted to have a charge less than +1. Variant effects on protein structure were then investigated using MD simulations. Root mean square deviation (RMSD) and radius of gyration (Rg) results indicated subtle SNV effects to the variant global structure in CA-II and CA-IV. However, with regards to CA-VIII RMSD analysis highlighted that variant presence was associated with increases to the structural rigidity of the protein. Principal component analysis (PCA) in conjunction with free energy analysis was performed to observe variant effects on protein conformational sampling in 3D space. The binding of BCT to CA-II induced greater protein conformational sampling and was associated with higher free energy. In CA-IV and CA-VIII PCA analysis revealed key differences in the mechanism of action of pathogenic and benign SNVs. In CA-IV, wild-type (WT) and benign variant protein structures clustered into single low energy well hinting at the presence of more stable structures. Pathogenic variants were associated with higher free energy and proteins sampled more conformations without settling into a low energy well. PCA analysis of CA-VIII indicated the opposite to CA-IV. Pathogenic variants were clustered into low energy wells, while the WT and benign variants showed greater conformational sampling. Dynamic cross correlation (DCC) analysis was performed using the MD-TASK suite to determine variant effects on residue movement. CA-II WT protein revealed that BCT and CO2 were associated with anti-correlated and correlated residue movement, highlighting at opposite mechanisms. In CA-IV and CA-VIII variant presence resulted in a change to residue correlation compared to the WT proteins. DRN analysis was performed to investigate SNV effects of residue accessibility and communication. Results demonstrated that SNVs are associated with allosteric effects on the CA protein structures, and effects are located on the stability assisting residues of the aromatic clusters and the active site of the proteins. CA-II studies discovered that Glu117 is the most important residue for communication, and variant presence results in a decrease to the usage of the residue. This effect was greatest in the CA-II H107Y SNV, and suggests that variants could have an effect on Zn2+ dissociation from the active site. Decreases to the usage of Zn2+ coordinating residues were also noted. Where this occurred, compensatory increases to the usage of other primary and secondary coordination residues were observed, that could possibly assist with the maintenance of Zn2+ within the active site. The CA-IV variants R69H and R219C highlighted potentially similar pathogenic mechanisms, whereas N86K and N177K hinted at potentially similar benign mechanisms. Within CA-VIII, variant presence was associated with changes to the accessibility of the N-terminal binding site residues. The benign CA-VIII variants highlighted possible compensatory mechanisms, whereby as one group of N-terminal residues loses accessibility, there was an increase to the accessibility of other binding site residues to possibly balance the effect. Catalytically, the proton shuttle residue His64 in CA-II was found to occupy a novel conformation named the “faux in” that brought the imidazole group even closer to the Zn2+ compared to the “in” conformation. Overall, compared to traditional MD simulations the incorporation of DRN allowed more detailed investigations into the variant mechanisms of action. This highlights the importance of network analysis in the study of the effects of missense mutations on the structure and function of proteins. Investigations of diseases at the molecular level is essential in the identification of disease pathogenesis and assists with the development of specifically tailored and better treatment options especially in the cases of genetically associated rare diseases.
- Full Text:
- Date Issued: 2020
Bioinformatics tool development with a focus on structural bioinformatics and the analysis of genetic variation in humans
- Authors: Brown, David K
- Date: 2018
- Subjects: Bioinformatics , Human genetics -- Variation , High performance computing , Workflow management systems , Molecular dynamics , Next generation sequencing , Human Mutation Analysis (HUMA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60708 , vital:27820
- Description: This thesis is divided into three parts, united under the general theme of bioinformatics tool development and variation analysis. Part 1 describes the design and development of the Job Management System (JMS), a workflow management system for high performance computing (HPC). HPC has become an integral part of bioinformatics. Computational methods for molecular dynamics and next generation sequencing (NGS) analysis, which require complex calculations on large datasets, are not yet feasible on desktop computers. As such, powerful computer clusters have been employed to perform these calculations. However, making use of these HPC clusters requires familiarity with command line interfaces. This excludes a large number of researchers from taking advantage of these resources. JMS was developed as a tool to make it easier for researchers without a computer science background to make use of HPC. Additionally, JMS can be used to host computational tools and pipelines and generates both web-based interfaces and RESTful APIs for those tools. The web-based interfaces can be used to quickly and easily submit jobs to the underlying cluster. The RESTful web API, on the other hand, allows JMS to provided backend functionality for external tools and web servers that want to run jobs on the cluster. Numerous tools and workflows have already been added to JMS, several of which have been incorporated into external web servers. One such web server is the Human Mutation Analysis (HUMA) web server and database. HUMA, the topic of part 2 of this thesis, is a platform for the analysis of genetic variation in humans. HUMA aggregates data from various existing databases into a single, connected and related database. The advantages of this are realized in the powerful querying abilities that it provides. HUMA includes protein, gene, disease, and variation data and can be searched from the angle of any one of these categories. For example, searching for a protein will return the protein data (e.g. protein sequences, structures, domains and families, and other meta-data). However, the related nature of the database means that genes, diseases, variation, and literature related to the protein will also be returned, giving users a powerful and holistic view of all data associated with the protein. HUMA also provides links to the original sources of the data, allowing users to follow the links to find additional details. HUMA aims to be a platform for the analysis of genetic variation. As such, it also provides tools to visualize and analyse the data (several of which run on the underlying cluster, via JMS). These tools include alignment and 3D structure visualization, homology modeling, variant analysis, and the ability to upload custom variation datasets and map them to proteins, genes and diseases. HUMA also provides collaboration features, allowing users to share and discuss datasets and job results. Finally, part 3 of this thesis focused on the development of a suite of tools, MD-TASK, to analyse genetic variation at the protein structure level via network analysis of molecular dynamics simulations. The use of MD-TASK in combination with the tools developed in the previous parts of this thesis is showcased via the analysis of variation in the renin-angiotensinogen complex, a vital part of the renin-angiotensin system.
- Full Text:
- Date Issued: 2018
- Authors: Brown, David K
- Date: 2018
- Subjects: Bioinformatics , Human genetics -- Variation , High performance computing , Workflow management systems , Molecular dynamics , Next generation sequencing , Human Mutation Analysis (HUMA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60708 , vital:27820
- Description: This thesis is divided into three parts, united under the general theme of bioinformatics tool development and variation analysis. Part 1 describes the design and development of the Job Management System (JMS), a workflow management system for high performance computing (HPC). HPC has become an integral part of bioinformatics. Computational methods for molecular dynamics and next generation sequencing (NGS) analysis, which require complex calculations on large datasets, are not yet feasible on desktop computers. As such, powerful computer clusters have been employed to perform these calculations. However, making use of these HPC clusters requires familiarity with command line interfaces. This excludes a large number of researchers from taking advantage of these resources. JMS was developed as a tool to make it easier for researchers without a computer science background to make use of HPC. Additionally, JMS can be used to host computational tools and pipelines and generates both web-based interfaces and RESTful APIs for those tools. The web-based interfaces can be used to quickly and easily submit jobs to the underlying cluster. The RESTful web API, on the other hand, allows JMS to provided backend functionality for external tools and web servers that want to run jobs on the cluster. Numerous tools and workflows have already been added to JMS, several of which have been incorporated into external web servers. One such web server is the Human Mutation Analysis (HUMA) web server and database. HUMA, the topic of part 2 of this thesis, is a platform for the analysis of genetic variation in humans. HUMA aggregates data from various existing databases into a single, connected and related database. The advantages of this are realized in the powerful querying abilities that it provides. HUMA includes protein, gene, disease, and variation data and can be searched from the angle of any one of these categories. For example, searching for a protein will return the protein data (e.g. protein sequences, structures, domains and families, and other meta-data). However, the related nature of the database means that genes, diseases, variation, and literature related to the protein will also be returned, giving users a powerful and holistic view of all data associated with the protein. HUMA also provides links to the original sources of the data, allowing users to follow the links to find additional details. HUMA aims to be a platform for the analysis of genetic variation. As such, it also provides tools to visualize and analyse the data (several of which run on the underlying cluster, via JMS). These tools include alignment and 3D structure visualization, homology modeling, variant analysis, and the ability to upload custom variation datasets and map them to proteins, genes and diseases. HUMA also provides collaboration features, allowing users to share and discuss datasets and job results. Finally, part 3 of this thesis focused on the development of a suite of tools, MD-TASK, to analyse genetic variation at the protein structure level via network analysis of molecular dynamics simulations. The use of MD-TASK in combination with the tools developed in the previous parts of this thesis is showcased via the analysis of variation in the renin-angiotensinogen complex, a vital part of the renin-angiotensin system.
- Full Text:
- Date Issued: 2018
Resource recovery options in brewery effluent treatment using activated sludge and high rate algal ponds: assessing environmental impacts
- Authors: Taylor, Richard Peter
- Date: 2020
- Subjects: Sewage -- Purification -- Activated sludge process , Sewage disposal plants , Sewage -- Purification -- Biological treatament , Sewage -- Purification -- Nitrogen removal , Brewery waste , Breweries -- Waste disposal , Microalgae -- Biotechnology , Algal biofuels
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/153746 , vital:39507
- Description: Wastewater treatment plants (WWTPs) are designed to clean effluents, but they also consume resources and produce waste. Various treatment technologies allow for the recovery of energy, nutrients and water from effluents turning this waste into products, which increases their sustainability and decreases the impact of WWTPs on the environment. There is a lack of literature which comprehensively compares the treatment performances, environmental impacts and beneficial downstream uses of the biomass generated by high rate algal pond (HRAP) and activated sludge (AS) treatment systems. This thesis aimed to compare (1) effluent treatment performance, (2) emissions and (3) downstream use of algae cultured in HRAP to sludge produced in AS and to obtain data to conduct a life cycle analysis (LCA) to compare the systems. The focus was on adding value to the effluent treatment process, while identifying the associated environmental impacts and contributing to the first ever zero-waste brewery effluent treatment system. Furthermore, these data were used to provide a basis to critically review and contribute to improving the methods used in the LCA of effluent treatment systems; particularly since this was the first wastewater treatment LCA that compared AS and HRAP using data collected from the same temporal and geographic location and from a single effluent stream. The electrical consumption water emission and land application of waste biomass caused the major environmental impacts of both treatment systems. The HRAP had less than 50 % of the electrical energy consumption (0.11±0.01 kW/m3 of effluent treated) compared to the AS system (0.29±0.11 kW/m3) which resulted in the technology having a lower climate change, photochemical oxidant formation, freshwater and marine ecotoxicity and fossil fuel depletion impact. It is imperative to understand the method of electrical energy (fossil fuel vs renewable) generation when conducting a LCA and deciding which technologies to use, since they have a major influence on the aforementioned impacts. The biogas yield of algal and sludge substrates was similar with an average gas production of 241 ml/g volatile solids fed. Biogas from algae fed digesters had a significantly higher methane content (64.73±0.81 %) and lower carbon dioxide content (22.94±0.24 %) when compared to WAS fed digesters (60.08±0.18 % and 27.37±0.43 %) respectively due to it being a less oxidised substrate. Swiss chard plants (Beta vulgaris) fertilised with anaerobically digested (AD) algae or sludge had a significantly higher mean biweekly yield (5.08±0.73 kg/m2) when compared to the inorganic-fertiliser control (3.45±0.89 kg/m2; p<0.0001). No difference was observed in the soil’s physical fertility when algae or sludge were applied to the soil (p>0.05). The HRAP produced more biomass (317.18±27.76 g/m3) than the AS (83.12±64.91 g/m3), which resulted in a significantly greater downstream production of biogas and fertiliser per volume of effluent treated. According to the LCA, this also resulted in the HRAP system having a higher terrestrial ecotoxicity, due to the greater volume of solids and thus heavy metals applied to the soil. This interpretation can be misleading, because the mass of heavy metals released into the environment is the same for both systems, with a greater portion being applied to the land in the HRAP scenario and discharged into fresh water in the case of AS. Future LCA models should clarify if these biomasses are going to be applied to a single piece of land or multiple sites as this will influence the risk of contamination via pollutant build up in the soil. The application of sludge or algae on soil increased the soil’s sodium concentration and sodium absorption ratio from 774.80±13.66 mg/kg to 952.17±34.89 mg/kg and 2.91±0.04 to 3.53±0.13, respectively. Regulations on the application of algae or sludge on agricultural soils should be altered to consider the limit values for sodium and future LCA’s associated with effluent treatment facilities should incorporate the possibility of soil contamination through sodium build-up. This work also conceptualised the importance of reporting water emissions in wastewater treatment LCA in as much detail as possible, because this had a significant influence on the eutrophication impacts on water systems. Reporting water emissions as total nitrogen underestimated downstream eutrophication impacts compared with those using nitrogen-species concentration (ammonia, nitrite, nitrate etc). A marine eutrophication sensitivity co-efficient should be included in future LCA models which accounts for the probability of nitrogen and phosphorus emissions entering the coastal environment as well as the vulnerability of the marine environment to eutrophication. Activated sludge systems are favourable for situations where space is limited, were there are inadequate options for biomass disposal (biomass not be used in agriculture or AD) and were electricity is generated from a renewable source; whereas, HRAP are more suitable under circumstances where electricity production relies on fossil fuel that carries a high environmental impact and where options are available to use the biomass for economic gain such as biogas and fertiliser production. This thesis contributes towards a zero-waste brewery effluent treated process. The HRAP and AS treated effluent for reuse in the brewery or in agricultural irrigation. The solids were anaerobically digested, and the carbon was recovered as a biogas, while the digestate was applied as an agricultural fertiliser. This allowed for the recovery of water, nutrients and carbon.
- Full Text:
- Date Issued: 2020
- Authors: Taylor, Richard Peter
- Date: 2020
- Subjects: Sewage -- Purification -- Activated sludge process , Sewage disposal plants , Sewage -- Purification -- Biological treatament , Sewage -- Purification -- Nitrogen removal , Brewery waste , Breweries -- Waste disposal , Microalgae -- Biotechnology , Algal biofuels
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/153746 , vital:39507
- Description: Wastewater treatment plants (WWTPs) are designed to clean effluents, but they also consume resources and produce waste. Various treatment technologies allow for the recovery of energy, nutrients and water from effluents turning this waste into products, which increases their sustainability and decreases the impact of WWTPs on the environment. There is a lack of literature which comprehensively compares the treatment performances, environmental impacts and beneficial downstream uses of the biomass generated by high rate algal pond (HRAP) and activated sludge (AS) treatment systems. This thesis aimed to compare (1) effluent treatment performance, (2) emissions and (3) downstream use of algae cultured in HRAP to sludge produced in AS and to obtain data to conduct a life cycle analysis (LCA) to compare the systems. The focus was on adding value to the effluent treatment process, while identifying the associated environmental impacts and contributing to the first ever zero-waste brewery effluent treatment system. Furthermore, these data were used to provide a basis to critically review and contribute to improving the methods used in the LCA of effluent treatment systems; particularly since this was the first wastewater treatment LCA that compared AS and HRAP using data collected from the same temporal and geographic location and from a single effluent stream. The electrical consumption water emission and land application of waste biomass caused the major environmental impacts of both treatment systems. The HRAP had less than 50 % of the electrical energy consumption (0.11±0.01 kW/m3 of effluent treated) compared to the AS system (0.29±0.11 kW/m3) which resulted in the technology having a lower climate change, photochemical oxidant formation, freshwater and marine ecotoxicity and fossil fuel depletion impact. It is imperative to understand the method of electrical energy (fossil fuel vs renewable) generation when conducting a LCA and deciding which technologies to use, since they have a major influence on the aforementioned impacts. The biogas yield of algal and sludge substrates was similar with an average gas production of 241 ml/g volatile solids fed. Biogas from algae fed digesters had a significantly higher methane content (64.73±0.81 %) and lower carbon dioxide content (22.94±0.24 %) when compared to WAS fed digesters (60.08±0.18 % and 27.37±0.43 %) respectively due to it being a less oxidised substrate. Swiss chard plants (Beta vulgaris) fertilised with anaerobically digested (AD) algae or sludge had a significantly higher mean biweekly yield (5.08±0.73 kg/m2) when compared to the inorganic-fertiliser control (3.45±0.89 kg/m2; p<0.0001). No difference was observed in the soil’s physical fertility when algae or sludge were applied to the soil (p>0.05). The HRAP produced more biomass (317.18±27.76 g/m3) than the AS (83.12±64.91 g/m3), which resulted in a significantly greater downstream production of biogas and fertiliser per volume of effluent treated. According to the LCA, this also resulted in the HRAP system having a higher terrestrial ecotoxicity, due to the greater volume of solids and thus heavy metals applied to the soil. This interpretation can be misleading, because the mass of heavy metals released into the environment is the same for both systems, with a greater portion being applied to the land in the HRAP scenario and discharged into fresh water in the case of AS. Future LCA models should clarify if these biomasses are going to be applied to a single piece of land or multiple sites as this will influence the risk of contamination via pollutant build up in the soil. The application of sludge or algae on soil increased the soil’s sodium concentration and sodium absorption ratio from 774.80±13.66 mg/kg to 952.17±34.89 mg/kg and 2.91±0.04 to 3.53±0.13, respectively. Regulations on the application of algae or sludge on agricultural soils should be altered to consider the limit values for sodium and future LCA’s associated with effluent treatment facilities should incorporate the possibility of soil contamination through sodium build-up. This work also conceptualised the importance of reporting water emissions in wastewater treatment LCA in as much detail as possible, because this had a significant influence on the eutrophication impacts on water systems. Reporting water emissions as total nitrogen underestimated downstream eutrophication impacts compared with those using nitrogen-species concentration (ammonia, nitrite, nitrate etc). A marine eutrophication sensitivity co-efficient should be included in future LCA models which accounts for the probability of nitrogen and phosphorus emissions entering the coastal environment as well as the vulnerability of the marine environment to eutrophication. Activated sludge systems are favourable for situations where space is limited, were there are inadequate options for biomass disposal (biomass not be used in agriculture or AD) and were electricity is generated from a renewable source; whereas, HRAP are more suitable under circumstances where electricity production relies on fossil fuel that carries a high environmental impact and where options are available to use the biomass for economic gain such as biogas and fertiliser production. This thesis contributes towards a zero-waste brewery effluent treated process. The HRAP and AS treated effluent for reuse in the brewery or in agricultural irrigation. The solids were anaerobically digested, and the carbon was recovered as a biogas, while the digestate was applied as an agricultural fertiliser. This allowed for the recovery of water, nutrients and carbon.
- Full Text:
- Date Issued: 2020
Investigating combinations of feature extraction and classification for improved image-based multimodal biometric systems at the feature level
- Authors: Brown, Dane
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63470 , vital:28414
- Description: Multimodal biometrics has become a popular means of overcoming the limitations of unimodal biometric systems. However, the rich information particular to the feature level is of a complex nature and leveraging its potential without overfitting a classifier is not well studied. This research investigates feature-classifier combinations on the fingerprint, face, palmprint, and iris modalities to effectively fuse their feature vectors for a complementary result. The effects of different feature-classifier combinations are thus isolated to identify novel or improved algorithms. A new face segmentation algorithm is shown to increase consistency in nominal and extreme scenarios. Moreover, two novel feature extraction techniques demonstrate better adaptation to dynamic lighting conditions, while reducing feature dimensionality to the benefit of classifiers. A comprehensive set of unimodal experiments are carried out to evaluate both verification and identification performance on a variety of datasets using four classifiers, namely Eigen, Fisher, Local Binary Pattern Histogram and linear Support Vector Machine on various feature extraction methods. The recognition performance of the proposed algorithms are shown to outperform the vast majority of related studies, when using the same dataset under the same test conditions. In the unimodal comparisons presented, the proposed approaches outperform existing systems even when given a handicap such as fewer training samples or data with a greater number of classes. A separate comprehensive set of experiments on feature fusion show that combining modality data provides a substantial increase in accuracy, with only a few exceptions that occur when differences in the image data quality of two modalities are substantial. However, when two poor quality datasets are fused, noticeable gains in recognition performance are realized when using the novel feature extraction approach. Finally, feature-fusion guidelines are proposed to provide the necessary insight to leverage the rich information effectively when fusing multiple biometric modalities at the feature level. These guidelines serve as the foundation to better understand and construct biometric systems that are effective in a variety of applications.
- Full Text:
- Date Issued: 2018
- Authors: Brown, Dane
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63470 , vital:28414
- Description: Multimodal biometrics has become a popular means of overcoming the limitations of unimodal biometric systems. However, the rich information particular to the feature level is of a complex nature and leveraging its potential without overfitting a classifier is not well studied. This research investigates feature-classifier combinations on the fingerprint, face, palmprint, and iris modalities to effectively fuse their feature vectors for a complementary result. The effects of different feature-classifier combinations are thus isolated to identify novel or improved algorithms. A new face segmentation algorithm is shown to increase consistency in nominal and extreme scenarios. Moreover, two novel feature extraction techniques demonstrate better adaptation to dynamic lighting conditions, while reducing feature dimensionality to the benefit of classifiers. A comprehensive set of unimodal experiments are carried out to evaluate both verification and identification performance on a variety of datasets using four classifiers, namely Eigen, Fisher, Local Binary Pattern Histogram and linear Support Vector Machine on various feature extraction methods. The recognition performance of the proposed algorithms are shown to outperform the vast majority of related studies, when using the same dataset under the same test conditions. In the unimodal comparisons presented, the proposed approaches outperform existing systems even when given a handicap such as fewer training samples or data with a greater number of classes. A separate comprehensive set of experiments on feature fusion show that combining modality data provides a substantial increase in accuracy, with only a few exceptions that occur when differences in the image data quality of two modalities are substantial. However, when two poor quality datasets are fused, noticeable gains in recognition performance are realized when using the novel feature extraction approach. Finally, feature-fusion guidelines are proposed to provide the necessary insight to leverage the rich information effectively when fusing multiple biometric modalities at the feature level. These guidelines serve as the foundation to better understand and construct biometric systems that are effective in a variety of applications.
- Full Text:
- Date Issued: 2018
Conceptualisations and pedagogical practices of academic literacy in Namibian higher education
- Authors: Julius, Lukas Homateni
- Date: 2021-04
- Subjects: Information literacy -- Study and teaching (Higher) -- Namibia , Academic writing -- Study and teaching -- Namibia , Qualitative research -- Methodology , Academic language -- Namibia , Information literacy -- Social aspects
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177293 , vital:42807 , 10.21504/10962/177293
- Description: The purpose of this study was to investigate academic literacy development lecturers’ conceptualisations of academic literacy and resultant pedagogical practices in academic development courses at three different Higher Education Institutional types in Namibia. The research sites were a Traditional University, a University of Technology and a Comprehensive University. The focus was to understand the extent to which the academics’ conceptions of academic literacy and the resultant pedagogical practices in the academic development courses at these three Higher Education Institutions (HEIs) facilitate epistemological access into students’ chosen fields of study. Bernstein’s Pedagogical theory (1990), Genre theory (1996) and Halliday’s Systemic Functional Linguistics (1978) were used as the study’s theoretical lenses and analytical framework. An interpretative paradigm and a qualitative case study design were employed as the research approach. Semi-structured interviews, classroom observations and documentary evidence were used to generate data. Research findings revealed a common (mis)conception of the nature of academic literacy, the resultant inadequate learning support offered to students in the selected academic literacy development courses, and a clear divorcing of academic literacy interventions from the students’ ‘home’ or mainstream disciplines at the three HEIs. The participants understood academic literacy from an autonomous position as a set of generic skills which could be taught outside of mainstream classes. Moreover, findings revealed that this understanding impacted on the design and assessments of all the academic literacy courses across the three universities under study. The study calls for a context sensitive model through which academic literacy acquisition can be scaffolded to meet the discipline-specific epistemological needs of the students. , Elalakano lyehokololoningomwa lyomapekapeko ndika olyo okukonakona ehumithokomeho lyomikalo dhokulesha nokushanga meilongngo lyopombada (oAcademic Literaci) maaputudhilongi, okukonakona omafatululo giisimanintsa moAcademic Literaci osho wo okutala iizemo yomikalo dhayooloka dhokulonga noku ilonga iilongwa yayooloka miiputudhilo yelongo lyopombada moNamibia. Omapekapeko ngaka oga li ga ningilwa miiputudhilo yomaukwatya ta ga landula; Oshiputudhiilo shopamudhigululwakalo, Oshiputudhilo shopaunongononi, nOshiputudilo shomailongo gaandjakana. Oshintsa shopokati shomapekapeko ngaka osho okuuva ko ondodo yowino osho wo euveko lyoAcademic Literaci maaputudhilongi nonkene euveko nontseyo ndjika tayi longithwa oku eta oshizemo tashi humitha komeho euveko lyopombanda lyaalongwa yomailongo geewino dhayooloka miiputudhilo itatu yelongo lyopombanda; shino otashi kwathele aalongwa yamone ontseyo ndjoka tayi ya kwathele meilongo lyawo. Omapekapeko ngano oga longitha omadhiladhiloukithi (eetheori) ga Bernstein’s Pedagogical theori (1990), Genre theori (1996) na Halliday’s Systemic Functional Linguistics theori (1978), mokufatulula nokundjandjukununa iizemo yomapekapeko. Omodela yokukonakona iizemo yongushu tayi ziilile maakonakonwa, oya tala ekonakono ndika onga oshintsa shopokati, oyo ya longithwa, opo ku monike uuyelele wothaathaa. Omikalo dha longifwa mokukonakona noku gongela uuyelele momapekapeko ngano ongaashi, eenkundathana dhayaali, omatalelo geetundi oshoyo omakonakono giinyanyangidhwa tayi kwandjangele nepekapeko ndika. Iizedjemo yepekapeko ndika otayi ulike kutya opena engwangwano montseyo nenge mefatululo lyuukwatya woAcademic Literaci, shoka sha eta enkundipalo meyambidhidho hali pewa aalongwa miilongwa yeewino dhayooloka. Shika otashi ulike kutya kapena etsokumwe pokati keenkambadhala tadhi ningwa kaapudhilongi dhokulonga oAcademic Litraci miilongwa ya yooloka mbyoka tayi ilongelwa kaalongwa miiputudhilo itatu yopombada. iizemmo yepekapeko olyo tuu mdika oya ulike wo kutya aalongwa mboka yaza komailongo ga yooloka oha yi ilongo nuudhigu opo ya pondole ondondo yomadhiladhilo gopombanda meilongo lyuukumwe. Mokukonakona euveko lyoAcademic Literaci, epekapeko ndika olya ndhindhilike kutya aakuthimbinga oyena euveko lyankundipala lyoterma ‘Academic Literaci,’ ano ya nyengwa okukwatakanitha oohedi dhopetameko ndhoka dhina oku ilongwa meikalekelo - ano pondje yiilongwa ikwao. Oshikwao, iizemo oya ulike kutya euveko ndika otali nwetha mo etungepo lyoAcademic Literaci onga oshilongwa, osho wo omakonakono gasho miiputudilo yombombanda itatu yakwatelwa momapekapeko. Hugunina, epekapeko ndika otali ulike/gandja oshiholelwa shomodela ndjoka oAcademic literacy tai vulu okulongwa opo yi kwatelemo eilongo lyiikwatelela kiilongwa osho yo komaitaalo nokeempumbwe dhaalongwa miiputudhilo yopombabda. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning (CHERTL), 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Julius, Lukas Homateni
- Date: 2021-04
- Subjects: Information literacy -- Study and teaching (Higher) -- Namibia , Academic writing -- Study and teaching -- Namibia , Qualitative research -- Methodology , Academic language -- Namibia , Information literacy -- Social aspects
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177293 , vital:42807 , 10.21504/10962/177293
- Description: The purpose of this study was to investigate academic literacy development lecturers’ conceptualisations of academic literacy and resultant pedagogical practices in academic development courses at three different Higher Education Institutional types in Namibia. The research sites were a Traditional University, a University of Technology and a Comprehensive University. The focus was to understand the extent to which the academics’ conceptions of academic literacy and the resultant pedagogical practices in the academic development courses at these three Higher Education Institutions (HEIs) facilitate epistemological access into students’ chosen fields of study. Bernstein’s Pedagogical theory (1990), Genre theory (1996) and Halliday’s Systemic Functional Linguistics (1978) were used as the study’s theoretical lenses and analytical framework. An interpretative paradigm and a qualitative case study design were employed as the research approach. Semi-structured interviews, classroom observations and documentary evidence were used to generate data. Research findings revealed a common (mis)conception of the nature of academic literacy, the resultant inadequate learning support offered to students in the selected academic literacy development courses, and a clear divorcing of academic literacy interventions from the students’ ‘home’ or mainstream disciplines at the three HEIs. The participants understood academic literacy from an autonomous position as a set of generic skills which could be taught outside of mainstream classes. Moreover, findings revealed that this understanding impacted on the design and assessments of all the academic literacy courses across the three universities under study. The study calls for a context sensitive model through which academic literacy acquisition can be scaffolded to meet the discipline-specific epistemological needs of the students. , Elalakano lyehokololoningomwa lyomapekapeko ndika olyo okukonakona ehumithokomeho lyomikalo dhokulesha nokushanga meilongngo lyopombada (oAcademic Literaci) maaputudhilongi, okukonakona omafatululo giisimanintsa moAcademic Literaci osho wo okutala iizemo yomikalo dhayooloka dhokulonga noku ilonga iilongwa yayooloka miiputudhilo yelongo lyopombada moNamibia. Omapekapeko ngaka oga li ga ningilwa miiputudhilo yomaukwatya ta ga landula; Oshiputudhiilo shopamudhigululwakalo, Oshiputudhilo shopaunongononi, nOshiputudilo shomailongo gaandjakana. Oshintsa shopokati shomapekapeko ngaka osho okuuva ko ondodo yowino osho wo euveko lyoAcademic Literaci maaputudhilongi nonkene euveko nontseyo ndjika tayi longithwa oku eta oshizemo tashi humitha komeho euveko lyopombanda lyaalongwa yomailongo geewino dhayooloka miiputudhilo itatu yelongo lyopombanda; shino otashi kwathele aalongwa yamone ontseyo ndjoka tayi ya kwathele meilongo lyawo. Omapekapeko ngano oga longitha omadhiladhiloukithi (eetheori) ga Bernstein’s Pedagogical theori (1990), Genre theori (1996) na Halliday’s Systemic Functional Linguistics theori (1978), mokufatulula nokundjandjukununa iizemo yomapekapeko. Omodela yokukonakona iizemo yongushu tayi ziilile maakonakonwa, oya tala ekonakono ndika onga oshintsa shopokati, oyo ya longithwa, opo ku monike uuyelele wothaathaa. Omikalo dha longifwa mokukonakona noku gongela uuyelele momapekapeko ngano ongaashi, eenkundathana dhayaali, omatalelo geetundi oshoyo omakonakono giinyanyangidhwa tayi kwandjangele nepekapeko ndika. Iizedjemo yepekapeko ndika otayi ulike kutya opena engwangwano montseyo nenge mefatululo lyuukwatya woAcademic Literaci, shoka sha eta enkundipalo meyambidhidho hali pewa aalongwa miilongwa yeewino dhayooloka. Shika otashi ulike kutya kapena etsokumwe pokati keenkambadhala tadhi ningwa kaapudhilongi dhokulonga oAcademic Litraci miilongwa ya yooloka mbyoka tayi ilongelwa kaalongwa miiputudhilo itatu yopombada. iizemmo yepekapeko olyo tuu mdika oya ulike wo kutya aalongwa mboka yaza komailongo ga yooloka oha yi ilongo nuudhigu opo ya pondole ondondo yomadhiladhilo gopombanda meilongo lyuukumwe. Mokukonakona euveko lyoAcademic Literaci, epekapeko ndika olya ndhindhilike kutya aakuthimbinga oyena euveko lyankundipala lyoterma ‘Academic Literaci,’ ano ya nyengwa okukwatakanitha oohedi dhopetameko ndhoka dhina oku ilongwa meikalekelo - ano pondje yiilongwa ikwao. Oshikwao, iizemo oya ulike kutya euveko ndika otali nwetha mo etungepo lyoAcademic Literaci onga oshilongwa, osho wo omakonakono gasho miiputudilo yombombanda itatu yakwatelwa momapekapeko. Hugunina, epekapeko ndika otali ulike/gandja oshiholelwa shomodela ndjoka oAcademic literacy tai vulu okulongwa opo yi kwatelemo eilongo lyiikwatelela kiilongwa osho yo komaitaalo nokeempumbwe dhaalongwa miiputudhilo yopombabda. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning (CHERTL), 2021
- Full Text:
- Date Issued: 2021-04
The effects of aspect, directional heating and depth on bedrock temperatures and the potential relationship with thermal fatigue weathering
- Authors: Breytenbach, Izak Johannes
- Date: 2021-04
- Subjects: Weathering -- South Africa -- Karoo , Shields (Geology) -- South Africa -- Karoo , Shields (Geology) -- Thermal properties , Tillite -- South Africa -- Karoo
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177688 , vital:42847 , 10.21504/10962/177688
- Description: Geomorphological weathering processes and their relationship with temperature fluctuations are frequently researched. These research efforts are mostly restricted to surface conditions where soils, loose-lying rocks or rock outcrops are assessed. However, there is a shortcoming in the understanding of the thermal properties in a rock mass, particularly in the upper metres below the surface, as this has not been investigated or researched in any great detail. The research reported here is unique in this regard as it exploited the use of rotary core boreholes in tillite bedrock in the Karoo (Western Cape, South Africa) which were drilled as part of an invasive materials investigation for a proposed new hard rock quarry to supply road stone. Temperature data were captured on a bedrock ridge hosting tillite outcrop with a strong east to west orientation. The site work was done in two separate phases. The initial phase of research saw data being captured for one year on two aspects (i.e. north and south) at a depth of 0.3 m. This was followed by the second (more detailed) phase, where three aspects were assessed (i.e. north, south and a flat crest), but at depths of 0.3 m, 2.0 m and 6.0 m, totalling nine data acquisition points. The second investigation phase gathered data for seven months. Supplementary weather data were also captured for the site. Subsequent data analyses revealed that there is much to learn about the thermal behaviour of bedrock in the upper metres below the surface, and that the temperature properties or thermal regimes are not as simple as often assumed. Temperature differences related to aspect are not only surficial, but are transmitted to underlying bedrock, and notable temperature differences were measurable to a depth of at least 6.0 m. Seasonal temperature fluctuations also have a pronounced effect on the rock mass temperatures and it was proven that deeper parts of the bedrock accumulate and dissipate heat at different (i.e. retarded) rates compared with shallower parts of the rock mass, creating suitable thermal conditions to induce thermal fatigue. Thermal properties were also proven to be non-linear. During analyses, the concept of temperature inversions was proven, whereby one part of the bedrock becomes hotter or cooler relative to another/adjacent part of the bedrock, compared with its earlier temperature state. It was argued that these temperature inversions may exacerbate thermal fatigue. This research proved that aspect (i.e. directional heating) and depth need to be considered when analysing thermal regimes in a rock mass, as these have a distinct influence. The effects which manifest themselves create suitable conditions for thermal fatigue, not only near the surface but also at depth in the bedrock. Lastly, the effects of meteorological conditions on shallow bedrock temperatures were assessed. Preliminary findings suggest that boundary conditions and meteorological processes may indeed affect shallow bedrock temperatures, but only under certain circumstances and not to the same extent as reported in the literature for conditions researched at the surface. Wind temperature relative to the rock temperature, as well as a combination of wind and rain were found to be the most significant factors. , Thesis (PhD) -- Faculty of Science, Geography, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Breytenbach, Izak Johannes
- Date: 2021-04
- Subjects: Weathering -- South Africa -- Karoo , Shields (Geology) -- South Africa -- Karoo , Shields (Geology) -- Thermal properties , Tillite -- South Africa -- Karoo
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177688 , vital:42847 , 10.21504/10962/177688
- Description: Geomorphological weathering processes and their relationship with temperature fluctuations are frequently researched. These research efforts are mostly restricted to surface conditions where soils, loose-lying rocks or rock outcrops are assessed. However, there is a shortcoming in the understanding of the thermal properties in a rock mass, particularly in the upper metres below the surface, as this has not been investigated or researched in any great detail. The research reported here is unique in this regard as it exploited the use of rotary core boreholes in tillite bedrock in the Karoo (Western Cape, South Africa) which were drilled as part of an invasive materials investigation for a proposed new hard rock quarry to supply road stone. Temperature data were captured on a bedrock ridge hosting tillite outcrop with a strong east to west orientation. The site work was done in two separate phases. The initial phase of research saw data being captured for one year on two aspects (i.e. north and south) at a depth of 0.3 m. This was followed by the second (more detailed) phase, where three aspects were assessed (i.e. north, south and a flat crest), but at depths of 0.3 m, 2.0 m and 6.0 m, totalling nine data acquisition points. The second investigation phase gathered data for seven months. Supplementary weather data were also captured for the site. Subsequent data analyses revealed that there is much to learn about the thermal behaviour of bedrock in the upper metres below the surface, and that the temperature properties or thermal regimes are not as simple as often assumed. Temperature differences related to aspect are not only surficial, but are transmitted to underlying bedrock, and notable temperature differences were measurable to a depth of at least 6.0 m. Seasonal temperature fluctuations also have a pronounced effect on the rock mass temperatures and it was proven that deeper parts of the bedrock accumulate and dissipate heat at different (i.e. retarded) rates compared with shallower parts of the rock mass, creating suitable thermal conditions to induce thermal fatigue. Thermal properties were also proven to be non-linear. During analyses, the concept of temperature inversions was proven, whereby one part of the bedrock becomes hotter or cooler relative to another/adjacent part of the bedrock, compared with its earlier temperature state. It was argued that these temperature inversions may exacerbate thermal fatigue. This research proved that aspect (i.e. directional heating) and depth need to be considered when analysing thermal regimes in a rock mass, as these have a distinct influence. The effects which manifest themselves create suitable conditions for thermal fatigue, not only near the surface but also at depth in the bedrock. Lastly, the effects of meteorological conditions on shallow bedrock temperatures were assessed. Preliminary findings suggest that boundary conditions and meteorological processes may indeed affect shallow bedrock temperatures, but only under certain circumstances and not to the same extent as reported in the literature for conditions researched at the surface. Wind temperature relative to the rock temperature, as well as a combination of wind and rain were found to be the most significant factors. , Thesis (PhD) -- Faculty of Science, Geography, 2021
- Full Text:
- Date Issued: 2021-04
The application of human factors and ergonomics (HFE) to community-sport organisations in resource scarce contexts: a case study of grassroots football in Makana, Eastern Cape
- Authors: Bennett, Ryan
- Date: 2021
- Subjects: Makana Local Football Association (South Africa) , Human engineering , Soccer teams -- South Africa -- Makhanda -- Management , Soccer -- Management -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/172412 , vital:42198 , 10.21504/10962/172412
- Description: Background: Modern Human Factors and Ergonomics (HFE) focuses on the optimisation of complex socio-technical systems and has been challenged to contribute to broader societal issues. An example is within grassroots football organisations in resource-scarce areas such as Makana, South Africa. Through embedded participatory approaches, the research problem was co-constructed: To investigate the socio-technical system of the Makana Local Football Association (LFA). Method: A useful complex system modelling tool is that of Cognitive Work Analysis (CWA), with its 5 phases used to identify constraints and affordances. Three perspectives were adopted for the application of an adapted (to suit participant characteristics) CWA to the Makana LFA: 1) how work is prescribed by the governing body SAFA, 2) how subject matter experts (SME) disclose its current functioning, and 3) how SMEs imagine it could function. Five SMEs attended 12 three-hour workshops to complete the latter two perspectives. Results: The composite work domain analysis between work as prescribed and work as disclosed identified significant mismatches between how policymakers envision the system and how SMEs report its functioning. Key differences in perspectives included the fundamental purpose of the Makana LFA, while only four of 22 functions operate within the Makana LFA. Participants also identified key affordances for the LFA such as reorientation as a community sports organisation. Discussion: Comparison between perspectives indicated four mismatches. 1) SAFA views the LFA as the foundation of the talent identification and development infrastructure of South African football. SMEs view it as a community centred organisation. 2) A lack of human capacity is evident at the community level of Makana football. 3) Funding and assets are absent at this grassroots level. 4) The LFA relies on other stakeholders, but these relationships are not formalised. Policymakers, therefore, have a lack of knowledge of the contextual challenges faced by LFA administrators. It is recommended that SAFA view the LFA as a community sport organisation, focusing on improving human capacity, increasing funding, and formalising stakeholder networks. Furthermore, conceptual models from CWA provide explicit socio-technical system redesign recommendations. Conclusion: Large mismatches between the organising body SAFA and the actual functioning of the LFA significantly hinder the effective management and running of football at a grassroots level in resource-scarce contexts in South Africa. The perspectives approach to CWA was useful in elucidating the constraints and affordances of the Makana LFA socio-technical system and informing redesign opportunities. Systems HFE methodology is therefore well placed to contribute to broader societal issues within resources scarce contexts such as football in Makana. Furthermore, the philosophical underpinnings of systems based HFE were successful in the development of sustainable participatory research within the South African grassroots football context.
- Full Text:
- Date Issued: 2021
- Authors: Bennett, Ryan
- Date: 2021
- Subjects: Makana Local Football Association (South Africa) , Human engineering , Soccer teams -- South Africa -- Makhanda -- Management , Soccer -- Management -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/172412 , vital:42198 , 10.21504/10962/172412
- Description: Background: Modern Human Factors and Ergonomics (HFE) focuses on the optimisation of complex socio-technical systems and has been challenged to contribute to broader societal issues. An example is within grassroots football organisations in resource-scarce areas such as Makana, South Africa. Through embedded participatory approaches, the research problem was co-constructed: To investigate the socio-technical system of the Makana Local Football Association (LFA). Method: A useful complex system modelling tool is that of Cognitive Work Analysis (CWA), with its 5 phases used to identify constraints and affordances. Three perspectives were adopted for the application of an adapted (to suit participant characteristics) CWA to the Makana LFA: 1) how work is prescribed by the governing body SAFA, 2) how subject matter experts (SME) disclose its current functioning, and 3) how SMEs imagine it could function. Five SMEs attended 12 three-hour workshops to complete the latter two perspectives. Results: The composite work domain analysis between work as prescribed and work as disclosed identified significant mismatches between how policymakers envision the system and how SMEs report its functioning. Key differences in perspectives included the fundamental purpose of the Makana LFA, while only four of 22 functions operate within the Makana LFA. Participants also identified key affordances for the LFA such as reorientation as a community sports organisation. Discussion: Comparison between perspectives indicated four mismatches. 1) SAFA views the LFA as the foundation of the talent identification and development infrastructure of South African football. SMEs view it as a community centred organisation. 2) A lack of human capacity is evident at the community level of Makana football. 3) Funding and assets are absent at this grassroots level. 4) The LFA relies on other stakeholders, but these relationships are not formalised. Policymakers, therefore, have a lack of knowledge of the contextual challenges faced by LFA administrators. It is recommended that SAFA view the LFA as a community sport organisation, focusing on improving human capacity, increasing funding, and formalising stakeholder networks. Furthermore, conceptual models from CWA provide explicit socio-technical system redesign recommendations. Conclusion: Large mismatches between the organising body SAFA and the actual functioning of the LFA significantly hinder the effective management and running of football at a grassroots level in resource-scarce contexts in South Africa. The perspectives approach to CWA was useful in elucidating the constraints and affordances of the Makana LFA socio-technical system and informing redesign opportunities. Systems HFE methodology is therefore well placed to contribute to broader societal issues within resources scarce contexts such as football in Makana. Furthermore, the philosophical underpinnings of systems based HFE were successful in the development of sustainable participatory research within the South African grassroots football context.
- Full Text:
- Date Issued: 2021
Adherence to antiretroviral therapy in children in Zimbabwe: a randomized control trial to validate a new self-reported adherence monitoring tool
- Authors: Mugore, Linnetie
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54734 , vital:26607
- Description: Background: Among children taking antiretroviral therapy (ART), self-reports have been widely reported to over-estimate adherence levels. Pill count adherence levels are often lower than self-reported levels, with unannounced home pill count adherence being lower than facility based pill count adherence. There is often poor agreement between pill count adherence levels and those measured using other objective adherence measuring methods such as Medication Event Monitoring Systems (MEMS®), which is widely viewed as the gold standard for adherence measurement. Objectives: The aim of this study was to design and evaluate a new self-reported paediatric adherence monitoring tool, assess the feasibility of using pill count methods in monitoring adherence and identify challenges to reporting adherence among children on ART in rural and urban Zimbabwe. Methods A dual centre, superiority, parallel design RCT was conducted to evaluate the newly-developed visually- and verbally-cued „past 10 days‟ tool for the assessment of adherence in children on ART at two sites in Zimbabwe; Harare Central Children‟s Hospital in an urban setting, and Murambinda Mission Hospital, a rural site. Child-caregiver pairs presenting to one of these facilities for the child‟s review of ART and refill of the medication were recruited, signed informed consent obtained, and were randomised for self-reported adherence monitoring into either the experimental group („new 10-day tool‟) or the control group („PACTG-style‟ self-report tool). Data (demographic, socioeconomic, and reported adherence) were collected in individual interviews with child-caregiver pairs. Additional adherence monitoring methods used for both groups included the Morisky-8-Item Medication Adherence Scale (MMAS-8) and a facility based pill count. FGDs were held with groups of caregivers and groups of children ≥13 years of age to understand reasons for non-adherence as well as issues around reporting non-adherence. Superiority testing was conducted by comparing adherent proportions and their confidence intervals (95% CI). Further concurrent validity test was done using the Mann-Whitney U test to evaluate the relationship between the new tool and the MMAS-8 scores. Agreement between the child and caregiver reports of adherence was used as a test of reliability of the new tool using the kappa statistic. Socio-demographic, clinical and care-related factors associated with adherence were identified using reported adherence in both child and caregiver groups in a logistic regression model. Two pill count methods were assessed for feasibility using the proportions of children with complete data for calculating adherence levels, and their CI and a comparison of the two methods, a routinely-used method and one that incorporated the reported residual quantity (RRQ) of medication at last refill. Results : Analysis included 245 child-caregiver pairs, 123 in the experimental group and 122 in the control group. The median age for children was 9 years. In the experimental group, adherence by caregiver and child reports ranged from 94.3% - 98.4% and 78.4% - 96.1%, and those in the control group ranged from 89.2% - 97.5% and 71.2% - 98.1%, respectively. There was no significant difference between adherence levels in the two groups. Adherence levels measured by both the experimental and control tools were found to be associated with MMAS-8 adherence levels (p <0.05). Agreement between child- and caregiver-reported adherence was moderate though significant (kappa; 0.407, p <0.05). Only about half of the children had adequate data to compute pill counts. Proportions adherent at 95% cut-off were 39% by the „routine pill count‟ and 58% by the „Pill count RRQ‟. Being an orphan was associated with child reported-adherence whereas use of non-human reminders, having a maternal relative as a primary caregiver and knowledge of dose frequency, were all associated with caregiver-reported adherence. Major causes of non-adherence mentioned during the FGDs included interference of medication administration times with scheduling of routine socio-economic activities and lack of support from some non-biological caregivers. Reporting of non-adherence appeared to be hampered by perceptions of negative reactions by healthcare workers to these reports and by caregivers being unaware that the child missed some doses. Conclusions: The „new 10-day tool‟ was not shown to be superior to the „PACTG-style tool‟ in detecting non-adherence, however this new tool was found to be a valid and reliable adherence monitoring tool that included a moderately long recall period of 10 days, can be applied without the need for the respondent to remember names of individual medicines in the
- Full Text:
- Date Issued: 2014
- Authors: Mugore, Linnetie
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54734 , vital:26607
- Description: Background: Among children taking antiretroviral therapy (ART), self-reports have been widely reported to over-estimate adherence levels. Pill count adherence levels are often lower than self-reported levels, with unannounced home pill count adherence being lower than facility based pill count adherence. There is often poor agreement between pill count adherence levels and those measured using other objective adherence measuring methods such as Medication Event Monitoring Systems (MEMS®), which is widely viewed as the gold standard for adherence measurement. Objectives: The aim of this study was to design and evaluate a new self-reported paediatric adherence monitoring tool, assess the feasibility of using pill count methods in monitoring adherence and identify challenges to reporting adherence among children on ART in rural and urban Zimbabwe. Methods A dual centre, superiority, parallel design RCT was conducted to evaluate the newly-developed visually- and verbally-cued „past 10 days‟ tool for the assessment of adherence in children on ART at two sites in Zimbabwe; Harare Central Children‟s Hospital in an urban setting, and Murambinda Mission Hospital, a rural site. Child-caregiver pairs presenting to one of these facilities for the child‟s review of ART and refill of the medication were recruited, signed informed consent obtained, and were randomised for self-reported adherence monitoring into either the experimental group („new 10-day tool‟) or the control group („PACTG-style‟ self-report tool). Data (demographic, socioeconomic, and reported adherence) were collected in individual interviews with child-caregiver pairs. Additional adherence monitoring methods used for both groups included the Morisky-8-Item Medication Adherence Scale (MMAS-8) and a facility based pill count. FGDs were held with groups of caregivers and groups of children ≥13 years of age to understand reasons for non-adherence as well as issues around reporting non-adherence. Superiority testing was conducted by comparing adherent proportions and their confidence intervals (95% CI). Further concurrent validity test was done using the Mann-Whitney U test to evaluate the relationship between the new tool and the MMAS-8 scores. Agreement between the child and caregiver reports of adherence was used as a test of reliability of the new tool using the kappa statistic. Socio-demographic, clinical and care-related factors associated with adherence were identified using reported adherence in both child and caregiver groups in a logistic regression model. Two pill count methods were assessed for feasibility using the proportions of children with complete data for calculating adherence levels, and their CI and a comparison of the two methods, a routinely-used method and one that incorporated the reported residual quantity (RRQ) of medication at last refill. Results : Analysis included 245 child-caregiver pairs, 123 in the experimental group and 122 in the control group. The median age for children was 9 years. In the experimental group, adherence by caregiver and child reports ranged from 94.3% - 98.4% and 78.4% - 96.1%, and those in the control group ranged from 89.2% - 97.5% and 71.2% - 98.1%, respectively. There was no significant difference between adherence levels in the two groups. Adherence levels measured by both the experimental and control tools were found to be associated with MMAS-8 adherence levels (p <0.05). Agreement between child- and caregiver-reported adherence was moderate though significant (kappa; 0.407, p <0.05). Only about half of the children had adequate data to compute pill counts. Proportions adherent at 95% cut-off were 39% by the „routine pill count‟ and 58% by the „Pill count RRQ‟. Being an orphan was associated with child reported-adherence whereas use of non-human reminders, having a maternal relative as a primary caregiver and knowledge of dose frequency, were all associated with caregiver-reported adherence. Major causes of non-adherence mentioned during the FGDs included interference of medication administration times with scheduling of routine socio-economic activities and lack of support from some non-biological caregivers. Reporting of non-adherence appeared to be hampered by perceptions of negative reactions by healthcare workers to these reports and by caregivers being unaware that the child missed some doses. Conclusions: The „new 10-day tool‟ was not shown to be superior to the „PACTG-style tool‟ in detecting non-adherence, however this new tool was found to be a valid and reliable adherence monitoring tool that included a moderately long recall period of 10 days, can be applied without the need for the respondent to remember names of individual medicines in the
- Full Text:
- Date Issued: 2014
“Ndingumfana osemncinci, kodwa ndizibonile izinto”
- Authors: Qambela, Gcobani
- Date: 2019
- Language: English , Xhosa
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92236 , vital:30694
- Description: Expected release date-April 2021
- Full Text: false
- Date Issued: 2019
- Authors: Qambela, Gcobani
- Date: 2019
- Language: English , Xhosa
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92236 , vital:30694
- Description: Expected release date-April 2021
- Full Text: false
- Date Issued: 2019
Energy use patterns and trends: the impact of energy policy in South African low-income households
- Israel-Akinbo, Sylvia Olawumi
- Authors: Israel-Akinbo, Sylvia Olawumi
- Date: 2018
- Subjects: Energy policy -- Social aspects -- South Africa , Energy security -- South Africa , Households -- Economic aspects -- South Africa , Poor -- Energy assistance -- South Africa , Poor -- South Africa , University of Cape Town. National Income Dynamics Study , Free Basic Electricity Policy (South Africa) , Income and Expenditure Survey (South Africa)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62264 , vital:28148
- Description: Energy poverty is a growing concern especially amongst low-income populations in developing countries. The transition to modern energy carriers is associated with welfare improvement and it is considered as an important developmental goal to achieve, in order to eradicate energy poverty. As such, the South African government has made energy poverty an issue of policy focus. Literature abounds with different measures of energy poverty; energy programmes and policies are also aimed at improving the welfare of the low-income households in terms of basic services such as electricity. As a point of entry into this study, this thesis explored energy use patterns and trends in low-income South African households. The research objectives addressed in the study included investigating the extent of energy poverty through a multidimensional energy poverty index, examining the extent to which the 'energy ladder' and 'energy stacking' models explains energy transition patterns and to examining whether the Free Basic Electricity Policy has impacted on energy choices and energy poverty. In order to contribute to energy poverty and energy policy discussion in South Africa, this study has investigated the dimensions of energy poverty amongst low-income South African households from these three different perspectives (objectives). A positivist approach, by using a quantitative method was used to underpin the study. The study utilised secondary data, which were from the National Income Dynamics Survey and Income and Expenditure Survey. The former was used for the analysis of the multidimensional energy poverty index and energy transition patterns whilst the latter was used for the analysis of the impact of the Free Basic Electricity Policy. The four waves of the National Income Dynamics Survey, with 2008 as the base year and the 2010/2011 version of the Income and Expenditure Survey were used whilst the data were processed through the quantitative software package, STATA version 12. The data were then analysed using the multidimensional energy poverty framework by Nussbaumer et al. (2011) and econometric models, which best fit the objectives. The result of the panel analysis, which assesses the multidimensional energy poverty for low-income households in South Africa showed that low-income households in both urban and rural areas are in a moderate state of energy poverty but different levels. However, the cross-sectional analysis revealed that the percentage of low-income households that are energy poor is reducing for the rural households but increasing for their urban counterparts from 2008 to 2014. The panel and cross-sectional results of the contribution of the energy dimensions in multidimensional energy poverty shows that the low-income households are especially energy poor in terms of heating fuel. The findings from the econometric estimates partly confirmed an energy ladder behaviour for the energy choice for cooking. An energy stacking behaviour was confirmed for the low- income households for these energy services - heating and lighting. Moreover, it was found that with respect to cooking and heating, low-income households living in a modern dwelling, having a small household size and residing in an urban area are less likely to use transitional or traditional energy carriers but most likely to use modern energy carriers. In addition, low-income households are more likely to use modern energy carriers for their cooking and heating in 2010, 2012 and 2014 than in 2008. For lighting energy service, low- income households living in a modern dwelling in an urban settlement are most likely to use modern energy carriers for lighting and less likely to use transitional energy carriers or candles. Only in 2014 were low-income households more likely to use modern energy carriers for lighting than in 2008. The results further showed that modern energy carriers have the highest probability of being preferred for lighting followed by cooking and lastly heating. Also emerging from the findings are that more urban low-income households are receiving the Free Basic Electricity (FBE) grant than their counterparts in the rural areas. The probability of low-income households owning entertainment/education appliance and food preserving appliance is positively influenced by access to Free Basic Electricity. The low- income households living in an urban settlement and in a modern type of dwelling supported this result. However, household size does not seems to support this result should it grow larger.
- Full Text:
- Date Issued: 2018
- Authors: Israel-Akinbo, Sylvia Olawumi
- Date: 2018
- Subjects: Energy policy -- Social aspects -- South Africa , Energy security -- South Africa , Households -- Economic aspects -- South Africa , Poor -- Energy assistance -- South Africa , Poor -- South Africa , University of Cape Town. National Income Dynamics Study , Free Basic Electricity Policy (South Africa) , Income and Expenditure Survey (South Africa)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62264 , vital:28148
- Description: Energy poverty is a growing concern especially amongst low-income populations in developing countries. The transition to modern energy carriers is associated with welfare improvement and it is considered as an important developmental goal to achieve, in order to eradicate energy poverty. As such, the South African government has made energy poverty an issue of policy focus. Literature abounds with different measures of energy poverty; energy programmes and policies are also aimed at improving the welfare of the low-income households in terms of basic services such as electricity. As a point of entry into this study, this thesis explored energy use patterns and trends in low-income South African households. The research objectives addressed in the study included investigating the extent of energy poverty through a multidimensional energy poverty index, examining the extent to which the 'energy ladder' and 'energy stacking' models explains energy transition patterns and to examining whether the Free Basic Electricity Policy has impacted on energy choices and energy poverty. In order to contribute to energy poverty and energy policy discussion in South Africa, this study has investigated the dimensions of energy poverty amongst low-income South African households from these three different perspectives (objectives). A positivist approach, by using a quantitative method was used to underpin the study. The study utilised secondary data, which were from the National Income Dynamics Survey and Income and Expenditure Survey. The former was used for the analysis of the multidimensional energy poverty index and energy transition patterns whilst the latter was used for the analysis of the impact of the Free Basic Electricity Policy. The four waves of the National Income Dynamics Survey, with 2008 as the base year and the 2010/2011 version of the Income and Expenditure Survey were used whilst the data were processed through the quantitative software package, STATA version 12. The data were then analysed using the multidimensional energy poverty framework by Nussbaumer et al. (2011) and econometric models, which best fit the objectives. The result of the panel analysis, which assesses the multidimensional energy poverty for low-income households in South Africa showed that low-income households in both urban and rural areas are in a moderate state of energy poverty but different levels. However, the cross-sectional analysis revealed that the percentage of low-income households that are energy poor is reducing for the rural households but increasing for their urban counterparts from 2008 to 2014. The panel and cross-sectional results of the contribution of the energy dimensions in multidimensional energy poverty shows that the low-income households are especially energy poor in terms of heating fuel. The findings from the econometric estimates partly confirmed an energy ladder behaviour for the energy choice for cooking. An energy stacking behaviour was confirmed for the low- income households for these energy services - heating and lighting. Moreover, it was found that with respect to cooking and heating, low-income households living in a modern dwelling, having a small household size and residing in an urban area are less likely to use transitional or traditional energy carriers but most likely to use modern energy carriers. In addition, low-income households are more likely to use modern energy carriers for their cooking and heating in 2010, 2012 and 2014 than in 2008. For lighting energy service, low- income households living in a modern dwelling in an urban settlement are most likely to use modern energy carriers for lighting and less likely to use transitional energy carriers or candles. Only in 2014 were low-income households more likely to use modern energy carriers for lighting than in 2008. The results further showed that modern energy carriers have the highest probability of being preferred for lighting followed by cooking and lastly heating. Also emerging from the findings are that more urban low-income households are receiving the Free Basic Electricity (FBE) grant than their counterparts in the rural areas. The probability of low-income households owning entertainment/education appliance and food preserving appliance is positively influenced by access to Free Basic Electricity. The low- income households living in an urban settlement and in a modern type of dwelling supported this result. However, household size does not seems to support this result should it grow larger.
- Full Text:
- Date Issued: 2018