http://vital.seals.ac.za:8080/vital/access/manager/Index ${session.getAttribute("locale")} 5 http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:70097 Wed 29 Nov 2023 11:14:39 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43873 Wed 24 Apr 2024 13:34:35 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50086 Wed 20 Apr 2022 13:28:53 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50203 Wed 15 May 2024 10:36:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50195 Wed 15 May 2024 10:35:22 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50196 Wed 15 May 2024 10:34:40 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44700 Wed 15 May 2024 10:34:09 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50087 Wed 15 May 2024 10:33:09 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28087 Wed 15 Mar 2023 09:37:04 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:71460 Wed 14 Aug 2024 15:27:45 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:71635 Wed 14 Aug 2024 09:02:55 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44734 Wed 10 Aug 2022 06:57:14 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50073 Wed 10 Aug 2022 05:39:17 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43155 Wed 09 Jun 2021 14:59:02 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:55699 Wed 08 May 2024 19:45:20 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44735 Wed 08 May 2024 15:04:12 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50090 Wed 08 May 2024 15:03:38 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44736 Wed 08 May 2024 15:03:07 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44711 Wed 08 May 2024 15:02:33 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50567 Wed 08 May 2024 15:02:05 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44727 Wed 08 May 2024 15:01:39 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44686 Wed 08 May 2024 14:56:39 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50209 Wed 08 May 2024 14:56:10 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74691 Wed 07 Aug 2024 15:55:21 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:72051 Wed 07 Aug 2024 15:48:21 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74687 Wed 07 Aug 2024 15:48:20 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74688 α-HCH > δ-HCH > β-HCH. Steric considerations and a molecular orbital theory approach can be used to rationalize the enantioselective nature of the catalysis based on the ease of approach of each Cl atom to the central Fe(I) ion and a consideration of the nodes on the C–Cl bonds that weaken these bonds in a manner that results in bond cleavage and the formation of an Fe–Cl bond.]]> Wed 07 Aug 2024 15:48:18 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44424 Wed 06 Oct 2021 23:29:46 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44425 Wed 06 Oct 2021 22:47:40 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74222 Tue 27 Aug 2024 16:02:13 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74212 Tue 27 Aug 2024 12:37:19 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74207 Tue 27 Aug 2024 12:36:59 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44684 Tue 24 May 2022 15:26:37 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50092 Tue 18 Jul 2023 22:13:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50071 Tue 18 Jul 2023 21:31:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:51101 Tue 17 May 2022 20:41:49 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44266 75 within 15 minutes. The effect of carbon nanotubes (as solid nano-sorbents) on the membrane performance and EME efficiency was evaluated. The method was linear over the range of 10-1000 ng/mL for zolpidem (R2 >0.9991) with repeatability (%RSD) between 0.3 % and 7.3 % (n = 3). The limits of detection and quantitation were 3 and 10 ng/mL, respectively. The sensitivity of HPLC-UV for the determination of zolpidem was enhanced by electromembrane extraction. Finally, the method was employed for the quantitation of zolpidem in biological samples with relative recoveries in the range of 60-79 %.]]> Tue 14 May 2024 11:52:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44709 Tue 12 Oct 2021 10:30:44 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44708 Tue 12 Oct 2021 10:18:27 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43136 Tue 08 Jun 2021 12:51:32 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43135 Tue 08 Jun 2021 12:16:42 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43133 Tue 08 Jun 2021 10:55:19 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43131 Tue 07 May 2024 14:06:40 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43153 Tue 07 May 2024 08:28:01 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:72948 Tue 04 Jun 2024 11:40:20 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50563 Tue 03 May 2022 10:22:28 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43340 Thu 29 Feb 2024 13:48:57 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74142 Thu 29 Aug 2024 16:20:49 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:71345 Thu 29 Aug 2024 09:23:06 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:68717 Thu 16 May 2024 05:21:52 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50556 Thu 11 Aug 2022 12:39:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44695 Thu 11 Aug 2022 12:37:38 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50206 Thu 11 Aug 2022 12:36:41 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28307 Thu 11 Apr 2024 19:25:38 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28430 Thu 09 Mar 2023 13:07:21 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28128 Thu 09 Mar 2023 13:00:25 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28428 Thu 09 Mar 2023 11:57:44 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28533 Thu 09 Mar 2023 09:44:15 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44414 Thu 07 Oct 2021 00:30:43 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:63979 Thu 04 May 2023 09:29:59 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44714 Sat 04 Dec 2021 12:22:30 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44713 Sat 04 Dec 2021 12:21:58 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44725 Sat 04 Dec 2021 12:21:07 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44682 Sat 04 Dec 2021 12:20:21 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44730 Sat 04 Dec 2021 12:19:30 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28298 Sat 01 Jul 2023 12:28:13 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:45517 Mon 26 Aug 2024 09:37:18 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43171 Mon 14 Jun 2021 09:34:29 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28063 90%) sequence similarity between BSCOMT and human soluble COMT (HSCOMT). BSCOMT was partially purified to 7.78 fold, 1.65% yield and had a specific activity of 0.052 U/mg. It had pH and temperature optima of 8.5 and 40oC, respectively. The Km, Vmax, Kcat and Kcat/Km towards esculetin methylation were respectively 1.475±0.130 pM, 0.0353±0.001 pmol/ml/min, 1.748 x 10-2±5.0x10-4 min-1 and 1.18x10-2 M-1. min-1. HSCOMT was expressed in Escherichia coli BL21(DE3) which showed optimal activity for esculetin methylation at pH and temperature of 7.0 and 30°C, respectively. It was purified to 5.62 fold, 22.6% yield with a specific activity of 3.85 U/mg. HSCOMT kinetic plots, upon incubation of the reaction mixture at 30°C for 5 min before addition of SAM was hyperbolic with Km, Vmax, Kcat and Kcat/Km values of 1.79 pM, 0.412 pmol/ml/min, 2.08 min-1 and 1.165 M-1. min-1, respectively. AuNPs and AgNPs showed a concentration dependent inhibition of HSCOMT activity upon increasing the 5 min incubation time to 1 h. Interestingly, HSCOMT kinetics, with 1 h incubation at 30°C, showed a sigmoidal curve, as well as increased activity. Incubation of the reaction mixture in the presence of 60 pM AuNPs and/or AgNPs for 1 hreversed the observed sigmoidal to a hyperbolic curve, with kinetic parameters comparable to those of 5 min incubation. SDS-PAGE analyses of HSCOMT after the kinetic experiments showed the enzyme incubated for 5 min as a monomer, while that which was incubated for 1 h migrated substantially as dimer. However, the HSCOMT incubated for 1 h in the presence of 60 pM AuNPs and/or AgNPs migrated as a monomer. This indicated that the extension of the incubation period allowed the dimerization of HSCOMT, which exhibited sigmoidal kinetics and higher activity. The presence of NPs impeded the HSCOMT dimerization which decreased the activity. Varying the concentration of SAM suggested that SAM had an allosteric modulatory effect on HSCOMT. Absorption spectroscopy indicated adsorption of HSCOMT on the gold and silver NP surfaces and the formation of NPs-HSCOMT corona. Fluorescence spectroscopy showed that the interaction of HSCOMT with both gold and silver NPs was governed by a static quenching mechanism, implying the formation of a non-fluorescent fluorophore-NP complex at the ground state. Further fluorometric analyses indicated that both gold and silver NPs had contact with Trp143; that the interactions were spontaneous and were driven by electrostatic interactions. Fourier transform infrared spectroscopic studies showed the adsorption of HSCOMT of the NPs surfaces to cause relaxation of the enzyme’s B-sheet structures. Molecular docking studies indicated involvement of largely hydrophilic amino acids, with the interacting distances of less than 3.5A. These findings signify the potential of nanotechnology in the control of COMT catalytic activity for the management of the COMT-related disorders.]]> Mon 13 Mar 2023 14:31:23 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28145 Mon 13 Mar 2023 12:53:24 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44705 Mon 11 Oct 2021 14:03:32 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44688 Mon 11 Oct 2021 11:06:34 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44681 Mon 11 Oct 2021 09:32:46 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43799 Mon 08 Aug 2022 09:12:14 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43192 Mon 08 Aug 2022 06:25:27 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43359 Mon 08 Aug 2022 06:24:01 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43353 Mon 08 Aug 2022 06:20:52 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43727 Mon 08 Aug 2022 06:19:08 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43179 Mon 08 Aug 2022 06:17:53 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43193 Mon 08 Aug 2022 05:52:29 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:43351 Mon 08 Aug 2022 05:46:20 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44578 Mon 06 May 2024 15:39:22 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44427 Mon 04 Oct 2021 19:59:57 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44428 Mon 04 Oct 2021 18:46:48 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44431 Mon 04 Oct 2021 13:48:57 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:44586 Mon 04 Oct 2021 11:22:01 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74962 Fri 30 Aug 2024 03:56:51 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:72092 Fri 26 Apr 2024 09:59:52 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:72114 Fri 26 Apr 2024 09:59:00 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:72089 Fri 26 Apr 2024 09:57:37 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:50205 Fri 22 Apr 2022 12:20:45 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:69400 Fri 10 May 2024 14:46:56 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:69385 Fri 10 May 2024 07:46:46 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28130 Fri 10 Mar 2023 15:27:16 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:28297 Fri 10 Mar 2023 12:46:15 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:45423 Fri 10 Mar 2023 10:05:17 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:68724 Fri 06 Oct 2023 12:59:55 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:68723 Fri 06 Oct 2023 12:56:49 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74578 Fri 02 Aug 2024 16:30:14 SAST ]]> http://vital.seals.ac.za:8080/vital/access/manager/Repository/vital:74579 Fri 02 Aug 2024 16:30:10 SAST ]]>