Anti-malarial synergy of secondary metabolites from Morinda lucida Benth.

Lakkakula, Jaya R; Matshaya, Thabo; Krause, Rui W M

Title: Anti-malarial synergy of secondary metabolites from Morinda lucida Benth.
Creator: Lakkakula, Jaya R
Creator: Matshaya, Thabo
Creator: Krause, Rui W M
Date Issued: 2017
Date: 2017
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/125609
Identifier: vital:35800
Identifier: https://doi.org/10.1016/j.msec.2016.08.073
Description: Ethnopharmacological relevance The roots, stem and leaves of Morinda lucida are used in some African countries as treatment against different types of fevers including yellow fever, malaria, trypanosomiasis and feverish conditions during child birth. Aim of the study To determine the in vitro cell toxicity and anti-malarial activity of the extracts of stem bark of M. lucida and to identify the secondary metabolites in the extract that may be responsible for this activity. Materials and methods The cell toxicity studies of crude extract [dichloromethane (DCM): Methanol (MeOH) in a ratio of1:1 (v/v)] as well as compounds isolated from the same extract were carried out using human cervix adenocarcinoma cells (HeLa cells); while the anti-malarial activities of the same samples were performed against Plasmodium falciparum strain 3D7 using the parasite lactate dehydrogenase (pLDH) assay. The isolation of the active compounds was carried out using chromatographic techniques (column and thin layer chromatography) where as mass spectrometry (MS), Fourier transform infrared spectroscopy (FTIR) as well as 1D- and 2D- nuclear magnetic resonance (NMR) analyses were employed in the characterisation and identification of the isolated secondary metabolites. Results The pLDH and cell toxicity assays for the crude extract and the fractions of M. lucida indicated that some fractions reduced the malaria parasite viability by approximately 50% at 100 μg/mL and they were not significantly cytotoxic. An IC50 done on the crude extract gave a value of 25 μg/mL. The % cell viability for the crude extract in cell toxicity assay remained at 100%. Seven chemical constituents i.e. asperuloside (1), asperulosidic acid (2), stigmasterol (3a), β-sitosterol (3b), cycloartenol (3c), campesterol (3d) and 5,15-O-dimethylmorindol (4) were isolated from the DCM-MeOH extract of stem bark. The isolated compounds tested were not that active by themselves individually at 20 μM but their activities were increased when the isolated compounds were combined. As seen when compounds 2, 3 and 4 (% viability: 93, 123 and 101 respectively) were combined yielding an IC50 value of 17 μM. Furthermore, this is the first report of compounds 1, 2, 3c, 3d and 4 isolated from M. lucida. Conclusion The crude extract completely suppressed the growth of P. falciparum. This indicates that the crude extract contains many compounds that might be acting in synergy. The observed activity of the crude extract and the samples containing a mixture of different compounds support the traditional use of M. lucida for the treatment of malaria.
Format: 8 pages
Format: pdf
Language: English
Relation: Materials Science and Engineering: C
Relation: Chithambo, B., Noundou, X.S. and Krause, R.W., 2017. Anti-malarial synergy of secondary metabolites from Morinda lucida Benth. Journal of ethnopharmacology, 199, pp.91-96.
Relation: Materials Science and Engineering: C number 70 169 177 2017 0928-4931
Rights: Materials Science and Engineering:C
Rights: Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)

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Krause, Rui Werner Maçedo (Prof)

RU Department of Chemistry

SDG 03. Good Health and Well-Being

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