- Title
- An investigation into bile formation of Wistar rats for excretion of injected gold nanoparticles
- Creator
- Thole, Sagoleka Thabo
- Subject
- Nanoparticles
- Subject
- Nanomedicine Gold -- Immunology Bile acids -- Physiological effect
- Date Issued
- 2019
- Date
- 2019
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10948/43900
- Identifier
- vital:37082
- Description
- There is an increase in the use of nanoparticles (NPs) throughout the world. Gold nanoparticles (AuNPs) are commonly used NPs in the field of biomedicine. NPs bigger than 8 nm are excreted via the hepatobiliary system. Bile acids (BAs) are regarded as the main components of bile. If there is a decreased or increased secretion of BAs, the composition of microbiota in the gut can change. Under normal physiological condition BAs fluctuates daily without causing any harm. Nevertheless, it is the long-term changes in bile acid secretion that can affect microbiota in the gut. If there is an increased excretion of AuNPs via hepatobiliary pathway it can alter bile formation. The aim of this study was to investigate whether the hepatobiliary excretion of AuNPs will alter the bile formation two weeks after intravenous administration of 14 nm AuNPs to Wistar rats. Rat liver samples of two previous experiments were used to investigate bile formation. The rats had been injected intravenously with a single dose of citrate capped and functionalised AuNPs. These rats were killed 14 days later. Small pieces of liver were stored in RNAlater. The mRNA of different enzymes and transporters in the hepatobiliary system were quantified in these samples and compared with suitable control. As a reference the mRNA of the same enzymes and transporters were extracted and quantified in rat liver that were killed one day after injection with one of the abovementioned functionalised AuNPs. The study shows down-regulation of genes responsible for conjugation of BAs whereas the rate-limiting enzymes were not significantly changed. This was the first study to investigate the formation of bile in relation to the use of AuNPs. The effect and consequences of AuNPs on the hepatobiliary system need further investigation.
- Format
- ix, 77 leaves
- Format
- Publisher
- Nelson Mandela University
- Publisher
- Faculty of Science
- Language
- English
- Rights
- Nelson Mandela University
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NMU School of BioMolecular and Chemical Sciences
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Sagoleka Thabo Thole.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |