The generation and characterisation of neutralising antibodies against the Theiler’s murine encephalomyelitis virus (TMEV) GDVII capsid reveals the potential binding site of the host cell co-receptor, heparan sulfate:

Upfold, Nicole; Ross, Caroline J; Tastan Bishop, Özlem; Luke, Garry A; Knox, Caroline M

Title: The generation and characterisation of neutralising antibodies against the Theiler’s murine encephalomyelitis virus (TMEV) GDVII capsid reveals the potential binding site of the host cell co-receptor, heparan sulfate:
Creator: Upfold, Nicole
Creator: Ross, Caroline J
Creator: Tastan Bishop, Özlem
Creator: Luke, Garry A
Creator: Knox, Caroline M
Date Issued: 2018
Date: 2018
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/148181
Identifier: vital:38717
Identifier: DOI: 10.1016/j.virusres.2017.11.017
Description: The early stages of picornavirus capsid assembly and the host factors involved are poorly understood. Since the localisation of viral proteins in infected cells can provide information on their function, antibodies against purified Theiler's murine encephalomyelitis virus (TMEV) GDVII capsids were generated by immunisation of rabbits. The resultant anti-TMEV capsid antibodies recognised a C-terminal region of VP1 but not VP2 or VP3 by Western analysis. Examination of the sites of TMEV capsid assembly by indirect immunofluorescence and confocal microscopy showed that at 5 h post infection, capsid signal was diffusely cytoplasmic with strong perinuclear staining and moved into large punctate structures from 6 to 8 h post infection.
Format: 11 pages
Format: pdf
Language: English
Relation: Virus research
Relation: Upfold, N., Ross, C., Bishop, Ö.T., Luke, G.A. and Knox, C., 2018. The generation and characterisation of neutralising antibodies against the Theiler’s murine encephalomyelitis virus (TMEV) GDVII capsid reveals the potential binding site of the host cell co-receptor, heparan sulfate. Virus research, 244, pp.153-163.
Relation: Virus research volume 244 number 4 153 163 January 2018 1872-7492
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Elsevier Terms and conditions statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)

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Knox, Caroline M (Prof)

Tastan Bishop, Ozlem (Prof)

RU Research Unit in Bioinformatics (RUBi)

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 03. Good Health and Well-Being

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