Electrochemical sensing and immunosensing using metallophthalocyanines and biomolecular modified surfaces

Mashazi, Philani Nkosinathi

Title: Electrochemical sensing and immunosensing using metallophthalocyanines and biomolecular modified surfaces
Creator: Mashazi, Philani Nkosinathi
Subject: Phthalocyanines
Subject: Electrochemistry
Subject: Electrodes, Enzyme
Subject: Measles -- Measurement
Date Issued: 2012
Date: 2012
Type: Thesis
Type: Doctoral
Type: PhD
Identifier: vital:5580
Identifier: http://hdl.handle.net/10962/d1018248
Description: The synthesis of cobalt and manganese phthalocyanine complexes bearing eight hexylthio and four amino substituents was carried out. The formation of thin films of these complexes using different modification methods was also studied. Hexylthio functionalized metallophthalocyanine complexes were immobilized onto gold electrode surfaces using the self-assembly techniques. Surface modifications using cobalt and manganese tetraamino phthalocyanine as polymers, monolayers (onto electrografted surfaces) and as carbon nanotube – metallophthalocyanine conjugates was also carried out. The new method of modifying gold electrodes with metal tetraamino phthalocyanine complexes was investigated. The modified electrode surfaces were studied for their electrocatalytic properties and as potential electrochemical sensors for the detection of hydrogen peroxide (H₂O₂). The limits of detection for the H₂O₂ were of the orders of ~10⁻⁷ M for all the modified electrodes. The modified electrodes gave very good analytical parameters; such as good sensitivity, linearity at studied concentration range and well-defined analytical peaks with increased current densities. The modification methods were reproducible, highly conducting thin films were formed and the modified electrodes were very stable. The design of electrochemical immunosensors for the detection of measles-specific antibodies was also carried out. The modified surface with measles-antigen as sensing element was accomplished using covalent immobilization for an intimate connection of the measles-antigen as a sensing layer onto an electrode surface. Two methods of detecting measles-specific antibodies were investigated and these methods were based on electrochemical impedance, i.e. label-free detection, and voltammetric method using horse-radish peroxidase (HRP) labeled antibody as a reporter. The detection of measles-specific antibodies was accomplished using both these methods. The potential applications of the designed immunosensor were evaluated in real samples (human and newborn calf serum) and the electrodes could detect the antibodies in the complex sample matrix with ease.
Format: 297 leaves
Format: pdf
Publisher: Rhodes University
Publisher: Faculty of Science, Chemistry
Language: English
Rights: Mashazi, Philani Nkosinathi

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Mashazi, Philani N (Associate Prof)

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