High performance liquid chromatographic analysis of oleandomycin in serum and urine

Title: High performance liquid chromatographic analysis of oleandomycin in serum and urine
Creator: Stubbs, Christopher
Creator: Haigh, John M
Creator: Kanfer, Isadore
Date Issued: 1986
Date: 1986
Type: text
Type: Article
Identifier: vital:6429
Identifier: http://hdl.handle.net/10962/d1006590
Description: The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
Format: 5 pages
Format: pdf
Language: English
Relation: Stubbs, C. and Haigh, J.M. and Kanfer, I. (1986) High performance liquid chromatographic analysis of oleandomycin in serum and urine. Journal of Chromatography, 353 . pp. 33-38. Available: http://dx.doi.org/doi:10.1016/S0021-9673(01)87074-...

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Kanfer, Isadore (Prof)

RU Department of Pharmacy

Haigh, JM (Prof)

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