Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp

Mayende, Lungisa; Wilhelmi, Brendan S; Pletschke, Brett I

Title: Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp
Creator: Mayende, Lungisa
Creator: Wilhelmi, Brendan S
Creator: Pletschke, Brett I
Date Issued: 2006
Date: 2006
Type: Article
Identifier: vital:6462
Identifier: http://hdl.handle.net/10962/d1005791
Identifier: http://dx.doi.org/10.1016/j.soilbio.2006.03.019
Description: In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml[superscript −1] min[superscript −1]. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml[superscript −1] min[superscript −1]. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.
Format: 6 pages
Format: pdf
Language: English
Relation: Mayende, L. and Wilhelmi, Brendan Shane and Pletschke, B.I. (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biology and Biochemistry, 38 (9). pp. 2963-2966. Available: http://dx.doi.org/10.1016/j.soilbio.2006.03.019

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Wilhelmi, Brendan (Dr)

Pletschke, Brett (Prof)

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 15. Life On Land

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