- Title
- The effect of physico-chemical parameters and chemical compounds on the activity of β-d-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water
- Creator
- Wutor, V C
- Creator
- Togo, C A
- Date Issued
- 2007
- Date
- 2007
- Type
- text
- Type
- Article
- Identifier
- vital:6470
- Identifier
- http://hdl.handle.net/10962/d1005800
- Identifier
- https://doi.org/10.1016/j.chemosphere.2007.02.050
- Description
- The presence of coliforms in polluted water was determined enzymatically (in situ) by directly monitoring the activity of beta-d-galactosidase (B-GAL) through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red beta-d-galactopyranoside (CPRG), which produced a red chlorophenol red (CPR) product. The objectives of this study were to monitor the effect of compounds commonly found in the environment and used in water treatment on a B-GAL CPRG assay and to investigate the differences between the environmental B-GAL enzyme and the pure commercial enzyme. Environmental B-GAL was optimally active at pH 7.8. Two temperature optima were observed at 35 and 55 degrees C, respectively. B-GAL activity was strongly inhibited by silver and copper ions. While calcium and ferrous ions at lower concentrations (50-100mgl(-1)) increased the enzyme activity, a reduction was observed at higher concentrations (200mgl(-1)). Sodium hypochlorite, normally used in rural areas to disinfect water gradually decreased B-GAL activity at concentrations between 0 and 5600ppm for both the commercial and environmental enzymes. B-GAL from the environment behaved differently from its commercially available counterpart.
- Format
- 4 pages
- Language
- English
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RU Department of Biochemistry, Microbiology and Bioinformatics
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