- Title
- Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyaninecobalt(II) tetraphenylporphyrin pentamer as a redox mediator
- Creator
- Ozoemena, Kenneth I
- Creator
- Nyokong, Tebello
- Date Issued
- 2006
- Date
- 2006
- Type
- Article
- Identifier
- vital:6583
- Identifier
- http://hdl.handle.net/10962/d1004148
- Description
- The development of cobalt(II) phthalocyanine–cobalt(II) tetra(5-phenoxy-10,15,20-triphenylporphyrin), (CoPc–(CoTPP)[subscript 4]) pentamer as a novel redox mediator for amperometric enzyme electrode sensitive to glucose is described. A glassy carbon electrode (GCE) was first modified with the pentamer, then followed by the immobilization onto the GCE–CoPc–(CoTPP)[subscript 4] with glucose oxidase (GOx) through cross-linking with glutaraldehyde in the presence of bovine serum albumin (BSA) and Nafion® cation-exchange polymer. The proposed biosensor displayed good amperometric respose charateristics to glucose in pH 7.0 PBS solution; such as low overpotentials (+400 mV versus Ag|AgCl), very fast amperometric response time (~5 s), linear concentration range extended up to 11 mM, with 10 μM detection limit. The biosensor exhibited electrochemical Michaelis–Menten kinetics and showed an average apparent Michaelis–Menten constant (K′M) of 14.91 ± 0.46 mM over a storage period of 2 weeks.
- Format
- 14 pages
- Format
- Language
- English
- Relation
- Ozoemena, K.I. and Nyokong, T. (2006) Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyanine–cobalt(II) tetraphenylporphyrin pentamer as a redox mediator. Electrochimica Acta, 51 (24). pp. 5131-5136. Available: http://dx.doi.org/10.1016/j.electacta.2006.03.055
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Nyokong, Tebello (Distinguished Professor)
RU Department of Chemistry
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