- Title
- Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA
- Creator
- Abdulkadir, Fatima
- Creator
- Knox, Caroline M
- Creator
- Marsberg, Tamryn
- Creator
- Hill, Martin P
- Creator
- Moore, Sean D
- Subject
- To be catalogued
- Date Issued
- 2015
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/417971
- Identifier
- vital:71498
- Identifier
- xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description
- Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (9 pages)
- Format
- Publisher
- SpringerLink
- Language
- English
- Relation
- BioControl
- Relation
- Abdulkadir, F., Knox, C., Marsberg, T., Hill, M.P. and Moore, S.D., 2015. Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA. BioControl, 60, pp.507-515
- Relation
- BioControl volume 60 p. 507 2015 1573-8248
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the SpringerLink Terms of Use Statement ( https://link.springer.com/termsandconditions)
- Rights
- Closed Access
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Collections
Knox, Caroline M (Prof)
RU DST/NRF SARChI for Insects in Sustainable Agricultural Ecosystems
Hill, Martin Patrick (Distinguished Professor)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
RU Department of Zoology and Entomology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA.pdf | 737 KB | Adobe Acrobat PDF | View Details Download |