- Title
- Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata)
- Creator
- Adeoyo, Olusegun R
- Creator
- Pletschke, Brett I
- Creator
- Dames, Joanna F
- Subject
- To be catalogued
- Date Issued
- 2018
- Date
- 2018
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/440405
- Identifier
- vital:73780
- Identifier
- https://doi.org/10.1186/s13568-018-0685-1
- Description
- This study aimed to purify and characterize amyloglucosidase (AMG) from Leohumicola incrustata. AMG was purified to homogeneity from cell-free culture filtrate of an ERM fungus grown in a modified Melin–Norkrans liquid medium. The molecular mass of the AMG was estimated to be 101 kDa by combining the results of Sephadex G-100 gel filtration, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, and zymography. The Km and kcat values were 0.38 mg mL−1 and 70 s−1, respectively, using soluble starch as a substrate. The enzyme was stable at 45 °C (pH 5.0), retaining over 65% activity after a pre-incubation period of 24 h. The metal inhibition profile of the AMG showed that Mn2+ and Ca2+ enhanced activity, while it was stable to metals ions, except a few (Al3+, Co2+, Hg2+ and Cd2+) that were inhibitory at a concentration higher than 5 mM. Thin layer chromatography revealed that only glucose was produced as the product of starch hydrolysis. The amylase from L. incrustata is a glucoamylase with promising characteristics such as temperature stability over an extended period, high substrate affinity and stability to a range of chemicals. Also, this study reports for the first time the possibility of using some culturable ERM fungi to produce enzymes for the bio-economy.
- Format
- 10 pages
- Format
- Language
- English
- Relation
- AMB Express
- Relation
- Adeoyo, O.R., Pletschke, B.I. and Dames, J.F., 2018. Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata). AMB Express, 8, pp.1-10
- Relation
- AMB Express volume 8 number 1 1 10 2018
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Springer Terms of Use Statement ( https://link.springer.com/termsandconditions)
- Hits: 125
- Visitors: 136
- Downloads: 17
Collections
Dames, Joanna Felicity (Prof)
Pletschke, Brett (Prof)
SDG 09. Industry, Innovation and Infrastructure
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata).pdf | 1 MB | Adobe Acrobat PDF | View Details Download |