A Mewome
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130981 , vital:36507 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-06
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130981 , vital:36507 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-06
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
A Mewome
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130954 , vital:36508 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-06
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130954 , vital:36508 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-06
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Abiban
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130914 , vital:36499 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-02
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130914 , vital:36499 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-02
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Armee Mali
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130959 , vital:36509 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-07
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130959 , vital:36509 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-07
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Atikoro
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130909 , vital:36498 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-01
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130909 , vital:36498 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-01
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Djourou
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130945 , vital:36506 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-05
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130945 , vital:36506 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-05
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Mali Dje
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130927 , vital:36503 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-03
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130927 , vital:36503 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-03
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Ndiyani
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130936 , vital:36504 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-04
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130936 , vital:36504 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-04
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Nten Nko File
- Sekou Kouyate (guitar, author, composer, arranger, lead vocal), Nanou Coul, Wande Kouyate, Yves Ndjock, Dieneba Seck (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Sekou Kouyate (guitar, author, composer, arranger, lead vocal) , Nanou Coul, Wande Kouyate, Yves Ndjock, Dieneba Seck (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130964 , vital:36510 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-08
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Sekou Kouyate (guitar, author, composer, arranger, lead vocal) , Nanou Coul, Wande Kouyate, Yves Ndjock, Dieneba Seck (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130964 , vital:36510 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-08
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Title unknown
- Dieneba Seck (lead vocal, chorus), Nanou Coul, Wande Kouyate, Yves Ndjock (chorus), Djely Moussa Kouyate (rhythm guitar), Tom Toumani Diakite (ngamole, ngomi, percussion), Thio Mbaye (percussion, djembe, tama), Maka Tounkara "Badie" (ngomi), Jean Luc Pinot (violon), Ben's Belinga (saxophone), Yves Ndjock (bass, snare drum, guitar), Syllart Production
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130986 , vital:36511 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-09
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
- Authors: Dieneba Seck (lead vocal, chorus) , Nanou Coul, Wande Kouyate, Yves Ndjock (chorus) , Djely Moussa Kouyate (rhythm guitar) , Tom Toumani Diakite (ngamole, ngomi, percussion) , Thio Mbaye (percussion, djembe, tama) , Maka Tounkara "Badie" (ngomi) , Jean Luc Pinot (violon) , Ben's Belinga (saxophone) , Yves Ndjock (bass, snare drum, guitar) , Syllart Production
- Date: 1998
- Subjects: Popular music , Popular music--Africa, West , Africa Cameroon Yaounde f-cm
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130986 , vital:36511 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC48-09
- Description: Traditional Malian music played on Malian percussion, violon and drums, accompanied with modern guitar, keyboard and brass, creating popular modern Malian music
- Full Text: false
- Date Issued: 1998
Bebe
- Amy Koita (lead vocal, author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130656 , vital:36464 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-04
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130656 , vital:36464 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-04
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Fali
- Amy Koita (lead vocal, author, composer, arranger), Allhassane Soumano (author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130683 , vital:36467 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-07
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130683 , vital:36467 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-07
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Kady
- Amy Koita (lead vocal, author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130670 , vital:36466 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-06
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130670 , vital:36466 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-06
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Kalo 9
- Amy Koita (lead vocal, author, composer, arranger), Allhassane Soumano (author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130665 , vital:36465 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-05
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130665 , vital:36465 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-05
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Sarama
- Amy Koita (lead vocal, author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130647 , vital:36463 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-03
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130647 , vital:36463 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-03
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Siraba
- Amy Koita (lead vocal, author, composer, arranger), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Maimouna Barry, Olisa (chorus), Thomas, Didier Deuirus, Manou Yodan (brass), Makan Tounkara (n'goni), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130688 , vital:36468 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-08
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Maimouna Barry, Olisa (chorus) , Thomas, Didier Deuirus, Manou Yodan (brass) , Makan Tounkara (n'goni) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130688 , vital:36468 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-08
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Yeko
- Amy Koita (lead vocal, author, composer, arranger), Allhassane Soumano (author, composer, arranger), Pamphile de Souza (programming), Moussa, Sidi Mady Gilbert (bass guitar), Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar), Sekouba, Aboubacar (percussion), Balake Cissoko (kora), Makan Tounkara (n'goni), Maimouna Barry, Olisa (chorus), Samir Naman
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Pamphile de Souza (programming) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Makan Tounkara (n'goni) , Maimouna Barry, Olisa (chorus) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130605 , vital:36444 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-01
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
- Authors: Amy Koita (lead vocal, author, composer, arranger) , Allhassane Soumano (author, composer, arranger) , Pamphile de Souza (programming) , Moussa, Sidi Mady Gilbert (bass guitar) , Zakaria, Djekoria Mory, Cissoko (solo and accompanying guitar) , Sekouba, Aboubacar (percussion) , Balake Cissoko (kora) , Makan Tounkara (n'goni) , Maimouna Barry, Olisa (chorus) , Samir Naman
- Date: 1999
- Subjects: Popular music , Popular music--Africa, West , Africa Mali Bamako f-ml
- Language: Bambara
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/130605 , vital:36444 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC45-01
- Description: Popular Malian Mandinga music accompanied by Mandinga percussion, kora, and n'goni traditional instruments fused with Western brass, keyboard and guitar
- Full Text: false
- Date Issued: 1999
Rhodeo, Vol. 22, No. 16
- Date: 1968-08-22
- Subjects: Grahamstown -- Newspapers , Journalism, Students -- South Africa , Rhodes University -- Activate , Rhodes University -- Students , Student newspapers and periodicals -- South Africa
- Language: English
- Type: Text
- Identifier: vital:14590 , http://hdl.handle.net/10962/d1019462
- Description: Rhodeo is the Independent Student Newspaper of Rhodes University. Located in Grahamstown, Rhodeo was established in 1947, and renamed in 1994 as Activate. During apartheid Rhodeo became an active part of the struggle for freedom of expression as part of the now defunct South African Student Press Union. Currently Activate is committed to informing Rhodes University students, staff and community members about relevant issues, mainly on campus. These issues range from hard news to more creative journalism. While Activate acts as a news source, one of its main objectives it to be accessible as a training ground for student journalists. The newspaper is run entirely by the students and is published twice a term. Activate is a free newspaper which receives an annual grant from the Rhodes University Student Representative Council, however, majority of its revenue is generated through advertising.
- Full Text:
- Date Issued: 1968-08-22
- Date: 1968-08-22
- Subjects: Grahamstown -- Newspapers , Journalism, Students -- South Africa , Rhodes University -- Activate , Rhodes University -- Students , Student newspapers and periodicals -- South Africa
- Language: English
- Type: Text
- Identifier: vital:14590 , http://hdl.handle.net/10962/d1019462
- Description: Rhodeo is the Independent Student Newspaper of Rhodes University. Located in Grahamstown, Rhodeo was established in 1947, and renamed in 1994 as Activate. During apartheid Rhodeo became an active part of the struggle for freedom of expression as part of the now defunct South African Student Press Union. Currently Activate is committed to informing Rhodes University students, staff and community members about relevant issues, mainly on campus. These issues range from hard news to more creative journalism. While Activate acts as a news source, one of its main objectives it to be accessible as a training ground for student journalists. The newspaper is run entirely by the students and is published twice a term. Activate is a free newspaper which receives an annual grant from the Rhodes University Student Representative Council, however, majority of its revenue is generated through advertising.
- Full Text:
- Date Issued: 1968-08-22
Rhodeo, Vol. 22, No. 17
- Date: 1968-08-29
- Subjects: Grahamstown -- Newspapers , Journalism, Students -- South Africa , Rhodes University -- Activate , Rhodes University -- Students , Student newspapers and periodicals -- South Africa
- Language: English
- Type: Text
- Identifier: vital:14591 , http://hdl.handle.net/10962/d1019463
- Description: Rhodeo is the Independent Student Newspaper of Rhodes University. Located in Grahamstown, Rhodeo was established in 1947, and renamed in 1994 as Activate. During apartheid Rhodeo became an active part of the struggle for freedom of expression as part of the now defunct South African Student Press Union. Currently Activate is committed to informing Rhodes University students, staff and community members about relevant issues, mainly on campus. These issues range from hard news to more creative journalism. While Activate acts as a news source, one of its main objectives it to be accessible as a training ground for student journalists. The newspaper is run entirely by the students and is published twice a term. Activate is a free newspaper which receives an annual grant from the Rhodes University Student Representative Council, however, majority of its revenue is generated through advertising.
- Full Text:
- Date Issued: 1968-08-29
- Date: 1968-08-29
- Subjects: Grahamstown -- Newspapers , Journalism, Students -- South Africa , Rhodes University -- Activate , Rhodes University -- Students , Student newspapers and periodicals -- South Africa
- Language: English
- Type: Text
- Identifier: vital:14591 , http://hdl.handle.net/10962/d1019463
- Description: Rhodeo is the Independent Student Newspaper of Rhodes University. Located in Grahamstown, Rhodeo was established in 1947, and renamed in 1994 as Activate. During apartheid Rhodeo became an active part of the struggle for freedom of expression as part of the now defunct South African Student Press Union. Currently Activate is committed to informing Rhodes University students, staff and community members about relevant issues, mainly on campus. These issues range from hard news to more creative journalism. While Activate acts as a news source, one of its main objectives it to be accessible as a training ground for student journalists. The newspaper is run entirely by the students and is published twice a term. Activate is a free newspaper which receives an annual grant from the Rhodes University Student Representative Council, however, majority of its revenue is generated through advertising.
- Full Text:
- Date Issued: 1968-08-29
Computational studies in human African trypanosomiasis
- Authors: Muronzi, Tendai
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431883 , vital:72812 , DOI 10.21504/10962/431885
- Description: Human African trypanosomiasis (HAT) is a neglected tropical disease (NTD) caused by two subspecies of the parasite, namely Trypanosoma brucei (Tb) gambiense (g-HAT) and rhodesiense (r-HAT). HAT is endemic in sub-Saharan countries, where the parasite transmission vectors, tsetse flies, breed. An estimated 70 million people remain at risk of contracting the disease, where the infection is classified as acute or chronic for g-HAT and r-HAT, respectively, with both forms ending in fatal meningoencephalitis when left untreated. Both g-HAT and r-HAT are responsible for widespread fatal epidemics throughout sub-Saharan African history, resulting from the complex molecular interplay between trypanosomes and humans through unique, innate immunity evasion mechanisms. Of interest, the Tbr subspecies expresses a serum resistance-associated protein (SRA), which binds to human serum lytic factor, apolipoprotein L1 (ApoL1), nullifying any trypanocidal activity. In response, ApoL1 (G1 and G2) variants found in humans of sub-Saharan African lineage have been cited for conferring resistance to the r-HAT infection in an interaction that is not fully elucidated In the event of successful infection, current HAT chemotherapeutics are plagued with complexity of administration, poor efficacy, toxicity, and potential drug resistance, highlighting a need for improved approaches. The parasite folate pathway provides a strategic target for alternative anti-trypanosomal drug development as trypanosomatids are folate auxotrophs, requiring host folate for growth and survival. Validated drug targets pteridine reductase (TbPTR1) and dihydrofolate reductase (TbDHFR) are essential for salvaging cofactors folate and folate biopterin crucial to parasite survival, making them viable targets for anti-folate investigation. The overall aims of this thesis were to a) provide insights into the molecular and dynamic basis of the SRA and ApoL1 interplay in HAT infection and b) identify safer and more efficient anti-folate anti-trypanosomal drug alternatives through in silico approaches. To achieve our first aim, in silico structure prediction was applied to generate 3D models of ApoL1 C-terminal variants G0, G1, G1G/M, G2 and G1G2, and four SRA variants retrieved from the NCBI database. The SRA and ApoL1 structures were inspected dynamically to identify the effect of the variants through molecular dynamics (MD) simulations. Dynamic residue network (DRN) analysis of MD trajectories was fundamental in identifying residues playing a vital role in the intramolecular communication of both proteins in the presence of mutations. Protein-protein docking was then applied to calculate plausible SRA-ApoL1 C-terminal wild-type complex structures to further elucidate the nature of SRA-mediated infection. Through MD simulations, twelve SRA-ApoL1 dimeric structures were narrowed down from five to two energetically sound complexes. The two feasible SRA-ApoL1 complexes (1 and 2) exhibited favourable communication observed through DRN analysis, including the retaining key communication residues identified in prior monomer DRN calculations. ApoL1 C-terminal variants were additionally incorporated into SRA-ApoL1 complexes 1 and 2 for further complex dynamics analysis This investigation into the nature of SRA-ApoL1 binding resulted in five primary outcomes: 1) highlighting the intramolecular effects ApoL1 variants have on the stability of the protein, 2) the identification of crucial SRA and ApoL1 communication residues in both monomeric or dimeric form, 3) the isolation of feasible SRA-ApoL1 complexes determined through global and local structural analyses 4) identification of residues crucial to the complex formation and maintenance of SRA-ApoL1, overlapping with those identified in (1), and 5) the minimal dissociative role of the G1 mutations in the complex, but compounding effect of the G2 deletion mutation. Computational modelling and drug repurposing were employed to achieve the thesis's second aim as they drastically cut down the costs involved in drug discovery and provide a more time-efficient screening method through numerous drug candidates. Using high throughput virtual screening, a subset of 2089 approved DrugBank compounds were screened against TbPTR1. The outputs were filtered to 24 viable compounds in 54 binding poses using binding energy and molecular interactions. Through subsequent MD simulations of 200ns, thirteen potential hit compounds were identified. The resultant hit compounds were subjected to further blind docking against TbDHFR and molecular dynamics to identify compounds with the potential for dual inhibition. The filtered subset was also tested in in vitro single concentration and dose-response bioassays to assess inhibitory properties against Trypanosoma brucei, complementing in silico findings. Post-molecular dynamics, four compounds exhibited high stabilities and molecular interactions with both TbPTR1 and TbDHFR, with two presenting favourable results in the in vitro assays. Three compounds additionally shared common structural moieties. In all, the in silico repurposing highlighted drugs characterised by favourable interactions and stabilities in TbPTR1, thus providing (1) a framework for further studies investigating anti-folate HAT compounds and (2) modulatory scaffolds based on identified moieties that can be used for the design of safe anti-folate trypanosomal drugs. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Muronzi, Tendai
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431883 , vital:72812 , DOI 10.21504/10962/431885
- Description: Human African trypanosomiasis (HAT) is a neglected tropical disease (NTD) caused by two subspecies of the parasite, namely Trypanosoma brucei (Tb) gambiense (g-HAT) and rhodesiense (r-HAT). HAT is endemic in sub-Saharan countries, where the parasite transmission vectors, tsetse flies, breed. An estimated 70 million people remain at risk of contracting the disease, where the infection is classified as acute or chronic for g-HAT and r-HAT, respectively, with both forms ending in fatal meningoencephalitis when left untreated. Both g-HAT and r-HAT are responsible for widespread fatal epidemics throughout sub-Saharan African history, resulting from the complex molecular interplay between trypanosomes and humans through unique, innate immunity evasion mechanisms. Of interest, the Tbr subspecies expresses a serum resistance-associated protein (SRA), which binds to human serum lytic factor, apolipoprotein L1 (ApoL1), nullifying any trypanocidal activity. In response, ApoL1 (G1 and G2) variants found in humans of sub-Saharan African lineage have been cited for conferring resistance to the r-HAT infection in an interaction that is not fully elucidated In the event of successful infection, current HAT chemotherapeutics are plagued with complexity of administration, poor efficacy, toxicity, and potential drug resistance, highlighting a need for improved approaches. The parasite folate pathway provides a strategic target for alternative anti-trypanosomal drug development as trypanosomatids are folate auxotrophs, requiring host folate for growth and survival. Validated drug targets pteridine reductase (TbPTR1) and dihydrofolate reductase (TbDHFR) are essential for salvaging cofactors folate and folate biopterin crucial to parasite survival, making them viable targets for anti-folate investigation. The overall aims of this thesis were to a) provide insights into the molecular and dynamic basis of the SRA and ApoL1 interplay in HAT infection and b) identify safer and more efficient anti-folate anti-trypanosomal drug alternatives through in silico approaches. To achieve our first aim, in silico structure prediction was applied to generate 3D models of ApoL1 C-terminal variants G0, G1, G1G/M, G2 and G1G2, and four SRA variants retrieved from the NCBI database. The SRA and ApoL1 structures were inspected dynamically to identify the effect of the variants through molecular dynamics (MD) simulations. Dynamic residue network (DRN) analysis of MD trajectories was fundamental in identifying residues playing a vital role in the intramolecular communication of both proteins in the presence of mutations. Protein-protein docking was then applied to calculate plausible SRA-ApoL1 C-terminal wild-type complex structures to further elucidate the nature of SRA-mediated infection. Through MD simulations, twelve SRA-ApoL1 dimeric structures were narrowed down from five to two energetically sound complexes. The two feasible SRA-ApoL1 complexes (1 and 2) exhibited favourable communication observed through DRN analysis, including the retaining key communication residues identified in prior monomer DRN calculations. ApoL1 C-terminal variants were additionally incorporated into SRA-ApoL1 complexes 1 and 2 for further complex dynamics analysis This investigation into the nature of SRA-ApoL1 binding resulted in five primary outcomes: 1) highlighting the intramolecular effects ApoL1 variants have on the stability of the protein, 2) the identification of crucial SRA and ApoL1 communication residues in both monomeric or dimeric form, 3) the isolation of feasible SRA-ApoL1 complexes determined through global and local structural analyses 4) identification of residues crucial to the complex formation and maintenance of SRA-ApoL1, overlapping with those identified in (1), and 5) the minimal dissociative role of the G1 mutations in the complex, but compounding effect of the G2 deletion mutation. Computational modelling and drug repurposing were employed to achieve the thesis's second aim as they drastically cut down the costs involved in drug discovery and provide a more time-efficient screening method through numerous drug candidates. Using high throughput virtual screening, a subset of 2089 approved DrugBank compounds were screened against TbPTR1. The outputs were filtered to 24 viable compounds in 54 binding poses using binding energy and molecular interactions. Through subsequent MD simulations of 200ns, thirteen potential hit compounds were identified. The resultant hit compounds were subjected to further blind docking against TbDHFR and molecular dynamics to identify compounds with the potential for dual inhibition. The filtered subset was also tested in in vitro single concentration and dose-response bioassays to assess inhibitory properties against Trypanosoma brucei, complementing in silico findings. Post-molecular dynamics, four compounds exhibited high stabilities and molecular interactions with both TbPTR1 and TbDHFR, with two presenting favourable results in the in vitro assays. Three compounds additionally shared common structural moieties. In all, the in silico repurposing highlighted drugs characterised by favourable interactions and stabilities in TbPTR1, thus providing (1) a framework for further studies investigating anti-folate HAT compounds and (2) modulatory scaffolds based on identified moieties that can be used for the design of safe anti-folate trypanosomal drugs. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13