A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories

Mohammadi, I; Kanfer, Isadore; Walker, Roderick B

Title: A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories
Creator: Mohammadi, I
Creator: Kanfer, Isadore
Creator: Walker, Roderick B
Date Issued: 2004
Date: 2004
Type: Article
Identifier: vital:6406
Identifier: http://hdl.handle.net/10962/d1006479
Description: Current compendial methods of assay for the analysis of cyclizine tablets involve the use of UV spectrophotometry. Since this is a non-selective technique its application to more complex dosage forms, such as suppositories, is unlikely to be appropriate. There is therefore a need for the development of a highly specific quantitative analytical method, such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE). The latter technique was chosen in view of some specific advantages over HPLC, such as the use of relatively non-toxic aqueous buffers, as opposed to organic solvents, which obviates the use of expensive HPLC grade solvents making CE more cost effective. Cyclizine was analyzed in 50 mM phosphate buffer (pH 2.3) and run at an applied voltage 25 kV. Detection sensitivity was enhanced by using a wavelength of 200 nm and samples were loaded hydrodynamically onto an uncoated fused-silica capillary (60 cm×50 mm i.d.). Chlorcyclizine was used as the internal standard and resolution of both compounds was achieved in less than 7 min. Stress testing was undertaken in order to investigate the appearance of breakdown products. The method has the requisite accuracy, selectivity, sensitivity and precision to assay cyclizine in tablets and suppositories. Degradation products resulting from the stress studies did not interfere with the detection of cyclizine and the assay is thus stability-indicating.
Format: 14 pages
Format: pdf
Language: English
Relation: Mohammadi, I. and Kanfer, I. and Walker, R.B. (2004) A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories. Journal of Pharmaceutical and Biomedical Analysis, 35 (1). pp. 233-239. Available: http://dx.doi.org/10.1016/j.jpba.2004.01.011

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Walker, Roderick Bryan (Prof)

Kanfer, Isadore (Prof)

RU Department of Pharmacy

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