Partial Purification and Characterization of Endoxylanase from a fungus, Leohumicola incrustata

Adeoyo, Olusegun R; Pletschke, Brett I; Dames, Joanna F

Title: Partial Purification and Characterization of Endoxylanase from a fungus, Leohumicola incrustata
Creator: Adeoyo, Olusegun R
Creator: Pletschke, Brett I
Creator: Dames, Joanna F
Subject: To be catalogued
Date Issued: 2021
Date: 2021
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/440393
Identifier: vital:73779
Identifier: 10.4314/br.v19i1.2
Description: Xylanases are glycoside hydrolases (GH) that degrade β-1, 4-xylan, a linear polysaccharide found as hemicellulose in cell wall of plants. Endoxylanase (Endo-1, 4-β-xylanase, EC 3.2. 1.8) randomly catalyses xylan to produce varying short xylooligosaccharides (XOS). This study aimed to determine the characteristics of a partially purified endoxylanase from Leohumicola incrustata. Enzyme production was carried out using beechwood (BW) xylan, after which the cell-free crude filtrate was concentrated using the ammonium sulphate precipitation method. The hydrolysed products were analysed by thin-layer chromatography (TLC) and zymography. The result showed that the enzyme produced varying smaller-sized linear xylooligosaccharides with R f values corresponding to those of xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose and other higher oligomers. The endoxylanase had a molecular mass of 72 kDa. The enzyme is stable in the presence of K+, Na+, Ca 2+, Fe 2+, Mg 2+, Zn 2+, Co 2+, pH of 5.0 and temperature of 37 o C. However, the activity gradually decreased after 60 min at 50 o C and retained over 69% activity after 120 min, while at 60 and 70 o C, the enzyme activity sharply decreased (pre-incubation periods). Endoxylanase from L. incrustata is comparable to those of other microorganisms and should be considered an attractive candidate for future industrial applications.
Format: 10 pages
Format: pdf
Language: English
Relation: Bio-Research
Relation: Adeoyo, O.R., Pletschke, B.I. and Dames, J.F., 2021. Partial Purification and Characterization of Endoxylanase from a fungus, Leohumicola incrustata. Bio-Research, 19(1), pp.1192-1201
Relation: Bio-Research volume 19 number 1 1192 1201 2021
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Bio-Research Statement (https://www.ajol.info/index.php/br)

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Dames, Joanna Felicity (Prof)

Pletschke, Brett (Prof)

RU Department of Biochemistry, Microbiology and Bioinformatics

SDG 09. Industry, Innovation and Infrastructure

SDG 15. Life On Land

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