Supplementary Material Synthesis and biological evaluation of (E)-cinnamic acid,(E)-2-styrylthiazole and (E)-2-[2-(naphthalen-1-yl) vinyl] thiazole derivatives
- Olawode, Emmanuel O, Tandlich, Roman, Prinsloo, Earl, Isaacs, Michelle, Hoppe, Heinrich C, Seldon, Ronnett, Warner, Digby F, Steenkamp, Vanessa, Kaye, Perry T
- Authors: Olawode, Emmanuel O , Tandlich, Roman , Prinsloo, Earl , Isaacs, Michelle , Hoppe, Heinrich C , Seldon, Ronnett , Warner, Digby F , Steenkamp, Vanessa , Kaye, Perry T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431778 , vital:72803 , xlink:href=" https://www.arkat-usa.org/get-file/59868/"
- Description: The screening was conducted using multi-well plates which are suited for HeLa cells in the log phase of growth with final cell density > 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.
- Full Text:
- Date Issued: 2016
- Authors: Olawode, Emmanuel O , Tandlich, Roman , Prinsloo, Earl , Isaacs, Michelle , Hoppe, Heinrich C , Seldon, Ronnett , Warner, Digby F , Steenkamp, Vanessa , Kaye, Perry T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431778 , vital:72803 , xlink:href=" https://www.arkat-usa.org/get-file/59868/"
- Description: The screening was conducted using multi-well plates which are suited for HeLa cells in the log phase of growth with final cell density > 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.
- Full Text:
- Date Issued: 2016
Dynamic mitochondrial localisation of STAT3 in the cellular adipogenesis model 3T3-L1:
- Kramer, Adam H, Edkins, Adrienne L, Hoppe, Heinrich C, Prinsloo, Earl
- Authors: Kramer, Adam H , Edkins, Adrienne L , Hoppe, Heinrich C , Prinsloo, Earl
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164885 , vital:41181 , DOI: 10.1002/jcb.25076
- Description: A mechanistic relationship exists between protein localisation, activity and cellular differentiation. Understanding the contribution of these molecular mechanisms is required for elucidation of conditions that drive development. Literature suggests non‐canonical translocation of the Signal Transducer and Activator of Transcription 3 (STAT3) to the mitochondria contributes to the regulation of the electron transport chain, cellular respiration and reactive oxygen species production. Based on this we investigated the role of mitochondrial STAT3, specifically the serine 727 phosphorylated form, in cellular differentiation using the well‐defined mouse adipogenic model 3T3-L1.
- Full Text:
- Date Issued: 2015
- Authors: Kramer, Adam H , Edkins, Adrienne L , Hoppe, Heinrich C , Prinsloo, Earl
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164885 , vital:41181 , DOI: 10.1002/jcb.25076
- Description: A mechanistic relationship exists between protein localisation, activity and cellular differentiation. Understanding the contribution of these molecular mechanisms is required for elucidation of conditions that drive development. Literature suggests non‐canonical translocation of the Signal Transducer and Activator of Transcription 3 (STAT3) to the mitochondria contributes to the regulation of the electron transport chain, cellular respiration and reactive oxygen species production. Based on this we investigated the role of mitochondrial STAT3, specifically the serine 727 phosphorylated form, in cellular differentiation using the well‐defined mouse adipogenic model 3T3-L1.
- Full Text:
- Date Issued: 2015
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