Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses

Reshamwala, Dhanik; Sheik Amamuddy, Olivier; Laquintana, Valentino; Denora, Nunzio; Zacheo, Antonella; Lampinen, Vili; Hytonen, Vesa P

Title: Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses
Creator: Reshamwala, Dhanik
Creator: Sheik Amamuddy, Olivier
Creator: Laquintana, Valentino
Creator: Denora, Nunzio
Creator: Zacheo, Antonella
Creator: Lampinen, Vili
Creator: Hytonen, Vesa P
Creator: Tastan Bishop, Özlem
Creator: Krol, Silke
Creator: Marjomäki, Varpu
Subject: To be catalogued
Date Issued: 2021
Date: 2021
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/476607
Identifier: vital:77941
Identifier: xlink:href="https://doi.org/10.3390/pharmaceutics13081182"
Description: To efficiently lower virus infectivity and combat virus epidemics or pandemics, it is important to discover broadly acting antivirals. Here, we investigated two naturally occurring polyphenols, Epigallocatechin gallate (EGCG) and Resveratrol (RES), and polyphenol-functionalized nanoparticles for their antiviral efficacy. Concentrations in the low micromolar range permanently inhibited the infectivity of high doses of enteroviruses (107 PFU/mL). Sucrose gradient separation of radiolabeled viruses, dynamic light scattering, transmission electron microscopic imaging and an in-house developed real-time fluorescence assay revealed that polyphenols prevented infection mainly through clustering of the virions into very stable assemblies. Clustering and stabilization were not compromised even in dilute virus solutions or after diluting the polyphenols-clustered virions by 50-fold. In addition, the polyphenols lowered virus binding on cells. In silico docking experiments of these molecules against 2- and 3-fold symmetry axes of the capsid, using an algorithm developed for this study, discovered five binding sites for polyphenols, out of which three were novel binding sites. Our results altogether suggest that polyphenols exert their antiviral effect through binding to multiple sites on the virion surface, leading to aggregation of the virions and preventing RNA release and reducing cell surface binding.
Format: computer
Format: online resource
Format: application/pdf
Format: 1 online resource (27 pages)
Format: pdf
Publisher: MDPI
Language: English
Relation: Pharmaceutics
Relation: Reshamwala D, Shroff S, Sheik Amamuddy O, Laquintana V, Denora N, Zacheo A, Lampinen V, Hytonen VP, Tastan Bishop Ö, Krol S, et al. Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses. Pharmaceutics. 2021; 13(8):1182
Relation: Pharmaceutics volume 13 number 8 p. 1182 2021 1999-4923
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Creative Commons CC BY-NC-ND 4.0 License (Attribution-NonCommercial-NoDerivatives 4.0 International) https://creativecommons.org/licenses/by-nc-nd/4.0/
Rights: Open Access

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Sheik Amamuddy, Olivier (Dr)

Tastan Bishop, Ozlem (Prof)

SDG 03. Good Health and Well-Being

RU Department of Biochemistry, Microbiology and Bioinformatics

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