Sing Hosanna (a medley of Praise)
- Peter Gabriels Muonagor (written, arranged, produced and composed by), Tosia Alesh (arranged and produced by), Studio 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129531 , vital:36303 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-01
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129531 , vital:36303 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-01
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
Oluwa Funmi
- Peter Gabriels Muonagor (written, arranged, produced and composed by), Tosia Alesh (arranged and produced by), Studio 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129536 , vital:36304 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-02
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129536 , vital:36304 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-02
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
Testimony
- Peter Gabriels Muonagor (written, arranged, produced and composed by), Tosia Alesh (arranged and produced by), Studio 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129549 , vital:36305 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-03
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
- Authors: Peter Gabriels Muonagor (written, arranged, produced and composed by) , Tosia Alesh (arranged and produced by) , Studio 2000
- Date: 2000
- Subjects: Popular music , Music--Religious aspects--Chrisitianity , Africa Nigeria Lagos f-nr
- Language: English , Yoruba
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/129549 , vital:36305 , International Library of African Music, Rhodes University, Grahamstown, South Africa , SDC29-03
- Description: Solo and chorus singers singing Christian gospel songs accompanied by Nigerian band using traditional Nigerian rhythms and instruments
- Full Text: false
- Date Issued: 2000
The design of a school model rural school for the rural Transkei, province of the Eastern Cape
- Authors: Van der Walt, Louwrens
- Date: 2016
- Subjects: School buildings -- South Africa -- Eastern Cape -- Designs and plans Elementary school buildings -- South Africa -- Transkei -- Design and construction , School buildings -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/15422 , vital:28243
- Description: This treatise investigates the issues and processes involved in the school provision systems used to provide schools in the former Transkei area of the Eastern Cape. The purpose of the treatise is to better understand the contextual, spatial, physical and theoretical nature of the rural areas at present of the Transkei and how these provision systems have responded to this. From this understanding a set of architectural issues are identified from which an appropriate building is designed, along with an appropriate provision system. Several precedents of provision systems used in the Transkei are investigated, along with several architectural precedents according to their particular responses. This is done in order to uncover the spatial relationships, material and physical aspects, as well as theoretical issues, from which an appropriate architectural language can be identified. The need for the provision systems to be investigated is established along with the identification of four sites within the Transkei area which are to be investigated. The selected site on which the design will be implemented is located on a tar road between Dutya and Willowvale as it has similar attributes to most existing school sites in the former Transkei and therefore functions as a good testing ground for developing an appropriate design that can be implemented on multiple sites. In order for the design of the provision system and building to be appropriate, an in-depth contextual analysis is done on the Transkei area as a whole from which a set of constraints and informants are derived to better guide the design.
- Full Text:
- Date Issued: 2016
- Authors: Van der Walt, Louwrens
- Date: 2016
- Subjects: School buildings -- South Africa -- Eastern Cape -- Designs and plans Elementary school buildings -- South Africa -- Transkei -- Design and construction , School buildings -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/15422 , vital:28243
- Description: This treatise investigates the issues and processes involved in the school provision systems used to provide schools in the former Transkei area of the Eastern Cape. The purpose of the treatise is to better understand the contextual, spatial, physical and theoretical nature of the rural areas at present of the Transkei and how these provision systems have responded to this. From this understanding a set of architectural issues are identified from which an appropriate building is designed, along with an appropriate provision system. Several precedents of provision systems used in the Transkei are investigated, along with several architectural precedents according to their particular responses. This is done in order to uncover the spatial relationships, material and physical aspects, as well as theoretical issues, from which an appropriate architectural language can be identified. The need for the provision systems to be investigated is established along with the identification of four sites within the Transkei area which are to be investigated. The selected site on which the design will be implemented is located on a tar road between Dutya and Willowvale as it has similar attributes to most existing school sites in the former Transkei and therefore functions as a good testing ground for developing an appropriate design that can be implemented on multiple sites. In order for the design of the provision system and building to be appropriate, an in-depth contextual analysis is done on the Transkei area as a whole from which a set of constraints and informants are derived to better guide the design.
- Full Text:
- Date Issued: 2016
Genotypic and phenotypic characterization of enterococci from cow dung and environmental water sources in three selected dairy farms in Amathole District
- Authors: Tanih, Godfred Ngu
- Date: 2016
- Subjects: Enterobacteriaceae Water -- Microbiology Enterococcus faecalis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2348 , vital:27755
- Description: Enterococcus species are integral members of the gastrointestinal microfloral of humans, animals, birds, as well as insects. Their presence in water and food has been greatly associated with faecal contamination. This study was aimed at evaluating the incidence of Enterococcus species in cow dung and environmental water sources in three commercial dairy farms. In addition, their antibiotic profiles were determined as well as resistance and virulence genes. Furthermore, the genetic relatedness of the isolates was determined by molecular typing method (RAPD PCR). Three hundred and thirty four water and faecal samples consisting of 117, 116 and 101 were collected from Seven Star Middle Drift and Fort Hare Dairy trusts respectively. Of the 334 samples collected, 289 were of faecal origin and 45 from water sources within the farms. All samples were screened for enterococci using culture base growth media and molecular methods targeting the tuf gene. Speciation was done using species-specific primers and the incidences of various species within the farms determined. Furthermore resistance to antibiotics and multidrug-resistant phenotypes were established using the disk diffusion method. Genes coding for virulence and resistance were also determined. From the samples collected, 313 (289 faecal and 24 water) presumptive enteroccocci were isolated, 305 of 313 (97.45 percent) were confirmed as Enterococcus of which 239 of 305 (78.38 percent) were identified as E. hirae, 15 of 305 (4.92 percent) as E. faecium, 12/305 (3.93 percent) as E. durans, 6 of 305 (1.97 percent) as E. faecalis and 33 of 305 (10.82 percent) were unidentified. Out of the five virulence genes that were targeted in the study only gelE (71.80 percent of 219/305) and ace (27.2 percent 83/305) were present in the isolates. Phenotypic resistance to antibiotics was observed is in all twelve antibiotics tested with multidrug resistance phenotypes detected in some enterococcal isolates most predominant in Seven Star and Middledrift dairy trust. Finally RAPD profiles of the isolates showed high relatedness between the strains from water and cow dung sources in all three commercial dairy farms suggesting possible contamination from cow dung to the water sources or vice versa.
- Full Text:
- Date Issued: 2016
- Authors: Tanih, Godfred Ngu
- Date: 2016
- Subjects: Enterobacteriaceae Water -- Microbiology Enterococcus faecalis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2348 , vital:27755
- Description: Enterococcus species are integral members of the gastrointestinal microfloral of humans, animals, birds, as well as insects. Their presence in water and food has been greatly associated with faecal contamination. This study was aimed at evaluating the incidence of Enterococcus species in cow dung and environmental water sources in three commercial dairy farms. In addition, their antibiotic profiles were determined as well as resistance and virulence genes. Furthermore, the genetic relatedness of the isolates was determined by molecular typing method (RAPD PCR). Three hundred and thirty four water and faecal samples consisting of 117, 116 and 101 were collected from Seven Star Middle Drift and Fort Hare Dairy trusts respectively. Of the 334 samples collected, 289 were of faecal origin and 45 from water sources within the farms. All samples were screened for enterococci using culture base growth media and molecular methods targeting the tuf gene. Speciation was done using species-specific primers and the incidences of various species within the farms determined. Furthermore resistance to antibiotics and multidrug-resistant phenotypes were established using the disk diffusion method. Genes coding for virulence and resistance were also determined. From the samples collected, 313 (289 faecal and 24 water) presumptive enteroccocci were isolated, 305 of 313 (97.45 percent) were confirmed as Enterococcus of which 239 of 305 (78.38 percent) were identified as E. hirae, 15 of 305 (4.92 percent) as E. faecium, 12/305 (3.93 percent) as E. durans, 6 of 305 (1.97 percent) as E. faecalis and 33 of 305 (10.82 percent) were unidentified. Out of the five virulence genes that were targeted in the study only gelE (71.80 percent of 219/305) and ace (27.2 percent 83/305) were present in the isolates. Phenotypic resistance to antibiotics was observed is in all twelve antibiotics tested with multidrug resistance phenotypes detected in some enterococcal isolates most predominant in Seven Star and Middledrift dairy trust. Finally RAPD profiles of the isolates showed high relatedness between the strains from water and cow dung sources in all three commercial dairy farms suggesting possible contamination from cow dung to the water sources or vice versa.
- Full Text:
- Date Issued: 2016
A critical evaluation of the human skin blanching assay and comparative bioavailability studies on topical corticosteroid preparations
- Authors: Meyer, Eric
- Date: 1989
- Subjects: Dermatopharmacology Skin, Effect of drugs on Adrenocortical hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3727 , http://hdl.handle.net/10962/d1001464
- Description: Several aspects of the human skin blanching assay were evaluated in an attempt to suggest improvements in the methodology of this assay. Three trials were performed in the unoccluded application mode, using two proprietary creams containing 0,1% betamethasone (as the 17-valerate). Preliminary observations of the influence of ambient temperature and relative humidity on the blanching response did not allow definite conclusions to be drawn. Studies on the number of observers required for reliable results of comparative blanching indicated that at least two trained observers should be employed. Analyses of the results of individual volunteers demonstrated the expected biological variability, and suggest that subjects selected for trials should represent a range of blanching responses. No sex-related differences in blanching responses were found, and both arms exhibited similar sensitivity to corticosteroids. Retrospective analysis of 95 040 observations of blanching responses showed that in the unoccluded application mode blanching is lowest close to the wrist, and in the occluded mode blanching is lowest close to the elbow. Studies on the method of transportation of Betnovate preparations suggest that topical formulations should not be exposed to temperature extremes during transportation. It is proposed that patients should not transport topical formulations in the holds of ships or aircraft, and that exporters and manufacturers should make use of special transportation and storage conditions. In a study of ten topical formulations from three countries it was found that there was no trend of products from one country consistently exhibiting superior blanching to products from the other two countries, or products from one country consistently exhibiting the lowest degree of blanching, although considerable differences in blanching responses were found in some cases. Interpretation of the results of these studies demonstrated the importance of employing a combination of statistical analyses, blanching profiles and AUC values when drawing conclusions regarding comparative bioavailability. A study of the blanching profiles of Betnovate cream included in all 16 trials performed during this work indicated that this preparation behaved in a similar fashion during all trials, thereby giving credence to the results of the trials
- Full Text:
- Date Issued: 1989
- Authors: Meyer, Eric
- Date: 1989
- Subjects: Dermatopharmacology Skin, Effect of drugs on Adrenocortical hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3727 , http://hdl.handle.net/10962/d1001464
- Description: Several aspects of the human skin blanching assay were evaluated in an attempt to suggest improvements in the methodology of this assay. Three trials were performed in the unoccluded application mode, using two proprietary creams containing 0,1% betamethasone (as the 17-valerate). Preliminary observations of the influence of ambient temperature and relative humidity on the blanching response did not allow definite conclusions to be drawn. Studies on the number of observers required for reliable results of comparative blanching indicated that at least two trained observers should be employed. Analyses of the results of individual volunteers demonstrated the expected biological variability, and suggest that subjects selected for trials should represent a range of blanching responses. No sex-related differences in blanching responses were found, and both arms exhibited similar sensitivity to corticosteroids. Retrospective analysis of 95 040 observations of blanching responses showed that in the unoccluded application mode blanching is lowest close to the wrist, and in the occluded mode blanching is lowest close to the elbow. Studies on the method of transportation of Betnovate preparations suggest that topical formulations should not be exposed to temperature extremes during transportation. It is proposed that patients should not transport topical formulations in the holds of ships or aircraft, and that exporters and manufacturers should make use of special transportation and storage conditions. In a study of ten topical formulations from three countries it was found that there was no trend of products from one country consistently exhibiting superior blanching to products from the other two countries, or products from one country consistently exhibiting the lowest degree of blanching, although considerable differences in blanching responses were found in some cases. Interpretation of the results of these studies demonstrated the importance of employing a combination of statistical analyses, blanching profiles and AUC values when drawing conclusions regarding comparative bioavailability. A study of the blanching profiles of Betnovate cream included in all 16 trials performed during this work indicated that this preparation behaved in a similar fashion during all trials, thereby giving credence to the results of the trials
- Full Text:
- Date Issued: 1989
A general genetic algorithm for one and two dimensional cutting and packing problems
- Authors: Mancapa, Vusisizwe
- Date: 2007
- Subjects: Packaging -- Data processing , Genetic algorithms , Cutting stock problem , Packing for shipment , Manufacturing processes -- Planning
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9602 , http://hdl.handle.net/10948/555 , http://hdl.handle.net/10948/d1011727 , Packaging -- Data processing , Genetic algorithms , Cutting stock problem , Packing for shipment , Manufacturing processes -- Planning
- Description: Cutting and packing problems are combinatorial optimisation problems. The major interest in these problems is their practical significance, in manufacturing and other business sectors. In most manufacturing situations a raw material usually in some standard size has to be divided or be cut into smaller items to complete the production of some product. Since the cost of this raw material usually forms a significant portion of the input costs, it is therefore desirable that this resource be used efficiently. A hybrid general genetic algorithm is presented in this work to solve one and two dimensional problems of this nature. The novelties with this algorithm are: A novel placement heuristic hybridised with a Genetic Algorithm is introduced and a general solution encoding scheme which is used to encode one dimensional and two dimensional problems is also introduced.
- Full Text:
- Date Issued: 2007
- Authors: Mancapa, Vusisizwe
- Date: 2007
- Subjects: Packaging -- Data processing , Genetic algorithms , Cutting stock problem , Packing for shipment , Manufacturing processes -- Planning
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9602 , http://hdl.handle.net/10948/555 , http://hdl.handle.net/10948/d1011727 , Packaging -- Data processing , Genetic algorithms , Cutting stock problem , Packing for shipment , Manufacturing processes -- Planning
- Description: Cutting and packing problems are combinatorial optimisation problems. The major interest in these problems is their practical significance, in manufacturing and other business sectors. In most manufacturing situations a raw material usually in some standard size has to be divided or be cut into smaller items to complete the production of some product. Since the cost of this raw material usually forms a significant portion of the input costs, it is therefore desirable that this resource be used efficiently. A hybrid general genetic algorithm is presented in this work to solve one and two dimensional problems of this nature. The novelties with this algorithm are: A novel placement heuristic hybridised with a Genetic Algorithm is introduced and a general solution encoding scheme which is used to encode one dimensional and two dimensional problems is also introduced.
- Full Text:
- Date Issued: 2007
Studies on acoustic properties of non-woven fabrics
- Authors: Mvubu, Mlando Basel
- Date: 2017
- Subjects: Needlepunch (Nonwoven fabric) Nonwoven fabrics , Textile fabrics
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19387 , vital:28866
- Description: This study is divided in to two main parts. The first part deals with the optimization of process parameters of needle-punched non-woven fabrics for achieving maximum sound absorption by employing a Box-Behnken factorial design. The influence of fibre type, depth of needle penetration and stroke frequency on sound absorption properties were studied. These parameters were varied at three levels during experimental trials. From multiple regression analysis, it was observed that the depth of needle penetration alone was the most dominant factor among the selected parameters, which was followed by the interaction between depth of needle penetration and stroke frequency. Fibre type was the least dominant parameter affecting sound absorption. A maximum sound absorption coefficient of 47% (0.47) was obtained from the selected parameters. The results showed that for a process such as needle-punching, which is influenced by multiple variables, it is important to also study the interactive effects of process parameters for achieving optimum sound absorption. The second part of the study deals with the effect of type of natural fibre (fineness), and the blending ratio (with PET fibres) on the air permeability of the needle-punched non-woven fabrics and then it proceeds to study the effect of the air-gap, type of natural fibre (fineness) and blending ratio (with PET fibres) on sound absorption of needle-punched non-woven fabrics. These parameters are tested individually and their two way interaction (synergy) effect using ANOVA. The air-gap was varied from 0mm to 25mm with 5mm increments, three natural fibre types were used and all were blended with polyester fibres at three blending ratios for each natural fibre type. The Univariate Tests of Significance shows that all three parameters have a significant effect on sound absorption together with two two-way interactions, with the exception of the Blend Ratio × Air Gap two-way interaction which was not significant. It was found that the sound absorption improves with the increase in the air-gap size up to 15mm after which sound absorption decreased slightly with the further increase in the air-gap up to 25mm.
- Full Text:
- Date Issued: 2017
- Authors: Mvubu, Mlando Basel
- Date: 2017
- Subjects: Needlepunch (Nonwoven fabric) Nonwoven fabrics , Textile fabrics
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19387 , vital:28866
- Description: This study is divided in to two main parts. The first part deals with the optimization of process parameters of needle-punched non-woven fabrics for achieving maximum sound absorption by employing a Box-Behnken factorial design. The influence of fibre type, depth of needle penetration and stroke frequency on sound absorption properties were studied. These parameters were varied at three levels during experimental trials. From multiple regression analysis, it was observed that the depth of needle penetration alone was the most dominant factor among the selected parameters, which was followed by the interaction between depth of needle penetration and stroke frequency. Fibre type was the least dominant parameter affecting sound absorption. A maximum sound absorption coefficient of 47% (0.47) was obtained from the selected parameters. The results showed that for a process such as needle-punching, which is influenced by multiple variables, it is important to also study the interactive effects of process parameters for achieving optimum sound absorption. The second part of the study deals with the effect of type of natural fibre (fineness), and the blending ratio (with PET fibres) on the air permeability of the needle-punched non-woven fabrics and then it proceeds to study the effect of the air-gap, type of natural fibre (fineness) and blending ratio (with PET fibres) on sound absorption of needle-punched non-woven fabrics. These parameters are tested individually and their two way interaction (synergy) effect using ANOVA. The air-gap was varied from 0mm to 25mm with 5mm increments, three natural fibre types were used and all were blended with polyester fibres at three blending ratios for each natural fibre type. The Univariate Tests of Significance shows that all three parameters have a significant effect on sound absorption together with two two-way interactions, with the exception of the Blend Ratio × Air Gap two-way interaction which was not significant. It was found that the sound absorption improves with the increase in the air-gap size up to 15mm after which sound absorption decreased slightly with the further increase in the air-gap up to 25mm.
- Full Text:
- Date Issued: 2017
Development of a reciprocating aerofoil wind energy harvester
- Authors: Phillips, Russell Leslie
- Date: 2008
- Subjects: Windmills , Wind power , Wind turbines
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:9622 , http://hdl.handle.net/10948/899 , Windmills , Wind power , Wind turbines
- Description: Cross flow wind turbines are not unique. The performance of Savonius and Darrieus turbines is well documented. Both share the advantage of being able to accept fluid flow from any direction. The Savonius is drag based and hence has poor power output while the Darrieus is lift based. Due to the fact that the Darrieus has fixed blades the fluid flow through the rotor does not result in optimal lift being generated at all points in the rotation circle. A drawback of the Darrieus system is that it has to operate at a high tip-to wind-speed ratio to obtain reasonable performance with the fixed blades. Deviation from a small optimal range of tip speed ratios results in poor performance. The Darrieus also has poor starting torque. The research conducted in this project focused on overcoming the shortcomings of other turbines and developing an effective cross flow turbine capable of good performance. A number of different concepts were experimented with, however all were based on a symmetrical aerofoil presented to the actual relative airflow at an angle that would produce the highest lift force at all times. The lift force was then utilized to generate movement and to do work on an electrical generator. All concepts contemplated were researched to ascertain their appropriateness for the intended application. During development of the final experimental platform and after lodging of a provisional patent (RSA 2007/00927) it was ascertained that the design shared some similarities with an American patent 5503525 dated 28/4/1994. This patent employed complex electronic sensing and control equipment for control of blade angle. This was thought to be overly complex and costly, particularly for small scale wind energy generation applications and a simpler mechanical solution was sought in the design of the final experimental platform used in this project. The design of the mechanical control system was refined in an attempt to make it simpler, more durable and employ the least number of moving parts. Literature studies and patent searches conducted, suggested that the mechanical control system as developed for the final experimental platform was unique. The enormous variation in the power available from the wind at the different wind speeds likely to be encountered by the device necessitated some means of control. In high wind conditions control of the amount of wind power into the device was deemed to be the preferable means of control. A number of different concepts to achieve this were devised and tested. The final concept employed limited the tail angle deflection and hence the lift produced by the aerofoils. This resulted in a seamless “throttle” control allowing the device to be used in any wind strength by adjusting the control to a position that resulted in the device receiving a suitable amount of power from the wind. The outcome of performance tests conducted indicated that the device has the potential to be developed into a viable wind turbine for both small and large scale applications. The ability to control the power input from the wind to the machine from zero to a maximum is considered to be one of the most beneficial outcomes of this project and together with the quiet operation and low speed, are considered the main advantages of the device over existing wind turbine designs. The possibilities of using the device to compress air for energy storage are exciting avenues that warrant further research.
- Full Text:
- Date Issued: 2008
- Authors: Phillips, Russell Leslie
- Date: 2008
- Subjects: Windmills , Wind power , Wind turbines
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:9622 , http://hdl.handle.net/10948/899 , Windmills , Wind power , Wind turbines
- Description: Cross flow wind turbines are not unique. The performance of Savonius and Darrieus turbines is well documented. Both share the advantage of being able to accept fluid flow from any direction. The Savonius is drag based and hence has poor power output while the Darrieus is lift based. Due to the fact that the Darrieus has fixed blades the fluid flow through the rotor does not result in optimal lift being generated at all points in the rotation circle. A drawback of the Darrieus system is that it has to operate at a high tip-to wind-speed ratio to obtain reasonable performance with the fixed blades. Deviation from a small optimal range of tip speed ratios results in poor performance. The Darrieus also has poor starting torque. The research conducted in this project focused on overcoming the shortcomings of other turbines and developing an effective cross flow turbine capable of good performance. A number of different concepts were experimented with, however all were based on a symmetrical aerofoil presented to the actual relative airflow at an angle that would produce the highest lift force at all times. The lift force was then utilized to generate movement and to do work on an electrical generator. All concepts contemplated were researched to ascertain their appropriateness for the intended application. During development of the final experimental platform and after lodging of a provisional patent (RSA 2007/00927) it was ascertained that the design shared some similarities with an American patent 5503525 dated 28/4/1994. This patent employed complex electronic sensing and control equipment for control of blade angle. This was thought to be overly complex and costly, particularly for small scale wind energy generation applications and a simpler mechanical solution was sought in the design of the final experimental platform used in this project. The design of the mechanical control system was refined in an attempt to make it simpler, more durable and employ the least number of moving parts. Literature studies and patent searches conducted, suggested that the mechanical control system as developed for the final experimental platform was unique. The enormous variation in the power available from the wind at the different wind speeds likely to be encountered by the device necessitated some means of control. In high wind conditions control of the amount of wind power into the device was deemed to be the preferable means of control. A number of different concepts to achieve this were devised and tested. The final concept employed limited the tail angle deflection and hence the lift produced by the aerofoils. This resulted in a seamless “throttle” control allowing the device to be used in any wind strength by adjusting the control to a position that resulted in the device receiving a suitable amount of power from the wind. The outcome of performance tests conducted indicated that the device has the potential to be developed into a viable wind turbine for both small and large scale applications. The ability to control the power input from the wind to the machine from zero to a maximum is considered to be one of the most beneficial outcomes of this project and together with the quiet operation and low speed, are considered the main advantages of the device over existing wind turbine designs. The possibilities of using the device to compress air for energy storage are exciting avenues that warrant further research.
- Full Text:
- Date Issued: 2008
A new gobiid fish, Fusigobius duospilus, from the tropical Indo-Pacific
- Hoese, Douglass F, Reader, Sally, J.L.B. Smith Institute of Ichthyology
- Authors: Hoese, Douglass F , Reader, Sally , J.L.B. Smith Institute of Ichthyology
- Date: 1985-01
- Subjects: Gobiidae -- Classification
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/70180 , vital:29631 , Margaret Smith Library (South African Institute for Aquatic Biodiversity (SAIAB)) Periodicals Margaret Smith Library (South African Institute for Aquatic Biodiversity (SAIAB))
- Description: Online version of original print edition of the Special Publication of the J.L.B. Smith Institute of Ichthyology; No. 36 , Fusigobius duospilusis described as a new species from several localities in the tropical Pacific and Indian oceans. This species differs from other species of Fusigobius in pectoral ray and gill-raker counts, coloration, reduced pelvic frenum, branching of the pelvic rays, presence of two large black spots on the first dorsal fin and shape of the first dorsal fin. The species is free living on sand. Males average a larger size than females. Sex ratios are uneven, with about twice as many females as males. Fusigobius longispinus Goren is recorded from several localities in the western Indian Ocean, where populations differ morphologically from Red Sea populations. Fusiogobius neophytus africanusls regarded as a synonym of F. neophytus.
- Full Text:
- Date Issued: 1985-01
- Authors: Hoese, Douglass F , Reader, Sally , J.L.B. Smith Institute of Ichthyology
- Date: 1985-01
- Subjects: Gobiidae -- Classification
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/70180 , vital:29631 , Margaret Smith Library (South African Institute for Aquatic Biodiversity (SAIAB)) Periodicals Margaret Smith Library (South African Institute for Aquatic Biodiversity (SAIAB))
- Description: Online version of original print edition of the Special Publication of the J.L.B. Smith Institute of Ichthyology; No. 36 , Fusigobius duospilusis described as a new species from several localities in the tropical Pacific and Indian oceans. This species differs from other species of Fusigobius in pectoral ray and gill-raker counts, coloration, reduced pelvic frenum, branching of the pelvic rays, presence of two large black spots on the first dorsal fin and shape of the first dorsal fin. The species is free living on sand. Males average a larger size than females. Sex ratios are uneven, with about twice as many females as males. Fusigobius longispinus Goren is recorded from several localities in the western Indian Ocean, where populations differ morphologically from Red Sea populations. Fusiogobius neophytus africanusls regarded as a synonym of F. neophytus.
- Full Text:
- Date Issued: 1985-01
The development potential of Kwazulu-Natal aquifers for rural water supply
- Authors: King, Georgina
- Date: 1997
- Subjects: Aquifers -- South Africa -- KwaZulu-Natal , Water-supply, Rural -- South Africa -- KwaZulu-Natal , Hydrology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4938 , http://hdl.handle.net/10962/d1005550 , Aquifers -- South Africa -- KwaZulu-Natal , Water-supply, Rural -- South Africa -- KwaZulu-Natal , Hydrology -- South Africa
- Description: The supply of water to 'disadvantaged' areas of KwaZulu-Natal has in the past received low priority. Local government is now faced with supplying water to large, sometimes dispersed, rural populations. Groundwater has been utilised informally as a water supply for some years, but the impetus provided by the Department of Water Affairs and Forestry's White Paper has compelled those responsible for water supply to seriously consider groundwater as a sustainable option. The development potential or success of groundwater in its role as a reliable water supply is dependent on acceptance of the resource by the communities, appropriate level of service, yield sustainability and safe quality. Apart from the social aspects, the yield and groundwater quality characteristics of the aquifers under consideration must be used to determine the best hydrogeological features to target during exploration. A total of 993 borehole records, from a recent government drought relief programme, were used to compare the yield, water quality and best geophysical exploration and drilling techniques of the main hydrolithologies in rural KwaZulu-Natal. The results of comparative analysis shows that the competent rocks of the Natal Group and Natal Metamorphic Province and the karstic Uloa Formation of the Maputaland Group have the best overall potential for water supply. The unconsolidated sediments of the Maputaland Group also have good potential, but have some salinity problems. The Karoo Supergroup sediments and volcanics have moderate potential, with the argillaceous rocks having the worst potential of the Karoo rocks. The contacts between the Ecca Group shales and sandstone have the best potential of the Karoo Supergroup sedimentary hydrolithologies. Fractures clearly enhance the groundwater potential of most hydrolithologies, with fractured Dwyka Group tillites rated as having one of the best development potentials of all the hydrogeological targets in KwaZulu-Natal, despite the hydrolithology's poor water-bearing characteristics. Dolerite contacts with sedimentary rocks are commonly targeted features in groundwater development. However, the results from this research showed that, apart from the Natal Group's contact with dolerite, these targets have poor development potential. In general, contacts between different hydro lithologies. Health related quality was found to be adversely affected in argillaceous hydrolithologies, such as the majority of Karoo rocks which had high levels of sodium and chloride and Natal Metamorphic Province schists which had elevated sodium, chloride and fluoride. Crystalline and arenaceous hydrolithologies generally exhibited good quality groundwater. A comparison between the different geophysical methods for each target feature indicates that there are appropriate methods to use to detect anomalies related to water-bearing features. The large number of dry boreholes drilled in locations with recorded geophysical anomalies can be either a function of the water-bearing characteristics of the formation, human error or background noise. The cost of using the different geophysical methods vary considerably. The order of increasing cost is magnetics, VLF, EM-34, electrical resistivity profiling followed by vertical electrical sounding. Drilling has a large influence on the development potential of certain aquifers due to the high costs involved. Most of the secondary aquifers will require percussion drilling which is the cheapest method of drilling commonly used. Some very unstable formations within fractured or highly weathered rock may need ODEX drilling to enable drilling to advance. ODEX drilling in these conditions is very costly and can double the cost of drilling compared to air percussion. The unconsolidated sediments of the Maputaland Group can only be drilled by mud rotary or ODEX techniques. The relative costs of these two methods arer very similar with ODEX being slightly cheaper. Because of the high expense of drilling in the sands it is recommended that alternative sources, possibly from shallow hand-dug wells, be considered as appropriate methods of accessing groundwater. The aspects of groundwater yield and quality of aquifers, appropriate geophysical siting and drilling methods, together with social considerations will all contribute to the success of groundwater development in rural KwaZulu-Natal.
- Full Text:
- Date Issued: 1997
- Authors: King, Georgina
- Date: 1997
- Subjects: Aquifers -- South Africa -- KwaZulu-Natal , Water-supply, Rural -- South Africa -- KwaZulu-Natal , Hydrology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4938 , http://hdl.handle.net/10962/d1005550 , Aquifers -- South Africa -- KwaZulu-Natal , Water-supply, Rural -- South Africa -- KwaZulu-Natal , Hydrology -- South Africa
- Description: The supply of water to 'disadvantaged' areas of KwaZulu-Natal has in the past received low priority. Local government is now faced with supplying water to large, sometimes dispersed, rural populations. Groundwater has been utilised informally as a water supply for some years, but the impetus provided by the Department of Water Affairs and Forestry's White Paper has compelled those responsible for water supply to seriously consider groundwater as a sustainable option. The development potential or success of groundwater in its role as a reliable water supply is dependent on acceptance of the resource by the communities, appropriate level of service, yield sustainability and safe quality. Apart from the social aspects, the yield and groundwater quality characteristics of the aquifers under consideration must be used to determine the best hydrogeological features to target during exploration. A total of 993 borehole records, from a recent government drought relief programme, were used to compare the yield, water quality and best geophysical exploration and drilling techniques of the main hydrolithologies in rural KwaZulu-Natal. The results of comparative analysis shows that the competent rocks of the Natal Group and Natal Metamorphic Province and the karstic Uloa Formation of the Maputaland Group have the best overall potential for water supply. The unconsolidated sediments of the Maputaland Group also have good potential, but have some salinity problems. The Karoo Supergroup sediments and volcanics have moderate potential, with the argillaceous rocks having the worst potential of the Karoo rocks. The contacts between the Ecca Group shales and sandstone have the best potential of the Karoo Supergroup sedimentary hydrolithologies. Fractures clearly enhance the groundwater potential of most hydrolithologies, with fractured Dwyka Group tillites rated as having one of the best development potentials of all the hydrogeological targets in KwaZulu-Natal, despite the hydrolithology's poor water-bearing characteristics. Dolerite contacts with sedimentary rocks are commonly targeted features in groundwater development. However, the results from this research showed that, apart from the Natal Group's contact with dolerite, these targets have poor development potential. In general, contacts between different hydro lithologies. Health related quality was found to be adversely affected in argillaceous hydrolithologies, such as the majority of Karoo rocks which had high levels of sodium and chloride and Natal Metamorphic Province schists which had elevated sodium, chloride and fluoride. Crystalline and arenaceous hydrolithologies generally exhibited good quality groundwater. A comparison between the different geophysical methods for each target feature indicates that there are appropriate methods to use to detect anomalies related to water-bearing features. The large number of dry boreholes drilled in locations with recorded geophysical anomalies can be either a function of the water-bearing characteristics of the formation, human error or background noise. The cost of using the different geophysical methods vary considerably. The order of increasing cost is magnetics, VLF, EM-34, electrical resistivity profiling followed by vertical electrical sounding. Drilling has a large influence on the development potential of certain aquifers due to the high costs involved. Most of the secondary aquifers will require percussion drilling which is the cheapest method of drilling commonly used. Some very unstable formations within fractured or highly weathered rock may need ODEX drilling to enable drilling to advance. ODEX drilling in these conditions is very costly and can double the cost of drilling compared to air percussion. The unconsolidated sediments of the Maputaland Group can only be drilled by mud rotary or ODEX techniques. The relative costs of these two methods arer very similar with ODEX being slightly cheaper. Because of the high expense of drilling in the sands it is recommended that alternative sources, possibly from shallow hand-dug wells, be considered as appropriate methods of accessing groundwater. The aspects of groundwater yield and quality of aquifers, appropriate geophysical siting and drilling methods, together with social considerations will all contribute to the success of groundwater development in rural KwaZulu-Natal.
- Full Text:
- Date Issued: 1997
Investigation into the characteristics and possible applications of biomass gasification by-products from a downdraft gasifier system
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
Rhodes University Library Annual Report 2011 : acting Library Director’s Review
- Authors: Berger, Jeanne
- Date: 2012
- Language: English
- Type: Text
- Identifier: vital:7944 , http://hdl.handle.net/10962/d1011891
- Description: [From the Introduction] 2011 saw the consolidation of a number of changes to Library services implemented in 2010 as a result of the 2009 Review of the Library Services Division. These include: 1) Collection development operating as a collaborative effort between Library staff and the academic community. The Collection Development Policy also underwent a major revision and was approved by Senate in October as the “Collection Development and Management Policy and Guidelines”. 2) The Faculty Liaison Service – the librarians in these teams are now proactively liaising with academics, for example, playing a crucial role in collection development for the departments, ensuring that they spend their information resources grants, creating awareness of appropriate online information resources and offering information literacy workshops.
- Full Text:
- Date Issued: 2012
- Authors: Berger, Jeanne
- Date: 2012
- Language: English
- Type: Text
- Identifier: vital:7944 , http://hdl.handle.net/10962/d1011891
- Description: [From the Introduction] 2011 saw the consolidation of a number of changes to Library services implemented in 2010 as a result of the 2009 Review of the Library Services Division. These include: 1) Collection development operating as a collaborative effort between Library staff and the academic community. The Collection Development Policy also underwent a major revision and was approved by Senate in October as the “Collection Development and Management Policy and Guidelines”. 2) The Faculty Liaison Service – the librarians in these teams are now proactively liaising with academics, for example, playing a crucial role in collection development for the departments, ensuring that they spend their information resources grants, creating awareness of appropriate online information resources and offering information literacy workshops.
- Full Text:
- Date Issued: 2012
Revision of the Indo-Pacific dottyback fish subfamily Pseudochrominae (Perciformes:Pseudochromidae)
- Authors: Gill, Anthony C
- Date: 2004
- Subjects: Fishes -- Indo-Pacific Region -- Classification , Pseudochromidae
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/137669 , vital:37548
- Description: The 100 nominal species in the pseudochromid subfamily Pseudochrominae are referred to 70 valid species, and an additional ten species are described as new. These species are assigned to ten genera: Assiculoides Gill & Hutchins, 1997, Assiculus Richardson, 1846, Cypho Myers, 1940, Labracinns Schlegel, 1858, Ogilbyina Fowler, 1931, Pseudochromis Riippell, 1835, and four new genera, Manonichthvs, Oxvcercichthys, Pholidochromis and Pictichromis. In previous recent studies only two genera, Labracinns and Pseudochromis , had been generally recognised; species assigned to the remaining genera had been previously placed in Pseudochromis. Species included in the subfamily are: Assiculoides desmonotus Gill & Hutchins, 1997 (Western Australia); Assiculus punctatus Richardson, 1846 (northwestern Australia); Cypho purpurascens (De Vis, 1884) (southwest Pacific); C. zaps sp. nov. (Indonesia to Ryukyu Ids); Labracinns atrofasciatus (Herre, 1933) (Culion, Philippines); L. cyclophthalmus (Muller & Troschel, 1849) (Japan to northwestern Australia, Papua New Guinea); L. lineatus (Castelnau, 1875) (Western Australia); Manonichthvs a/leni sp. nov. (Sabah, Borneo); M. paranox (Lubbock & Goldman, 1976) (Solomon Ids, Papua New Guinea, Great Barrier Reef); M. polynemus (Fowler, 1931) (northeastern Indonesia, Belau); M. splendens (Fowler, 1931) (southeastern Indonesia); M. winterbottomi sp nov. (Cebu, Philippines); Ogilbyina novaehollandiae (Steindachner, 1880) (southern Great Barrier Reef and Queensland); O. queenslandiae (Saville-Kent, 1893) (Queensland, Great Barrier Reef); O. salvati (Plessis & Fourmanoir, 1966) (New Caledonia); Oxvcercichthys veliferus (Lubbock, 1980) (Great Barrier Reef, western Coral Sea); Pholidochromis marginata (Lubbock, 1980) (northeastern Indonesia to Bougainville); Pictichromis aurifrons (Lubbock, 1980) (New Guinea); P. coralensis sp. nov. (Great Barrier Reef to New Caledonia); P. diadema (Lubbock & Randall, 1978) (Malaysia to Philippines and northern Borneo); P. ephippiata (Gill, Pyle & Earle, 1996) (northern Sulawesi, southeastern Papua New Guinea); P. paccagnellae (Axelrod, 1973) (Indonesia, Timor Sea to Solomon Ids); P. porphyrea (Lubbock & Goldman, 1974) (Ryukyu Ids and northeastern Indonesia to Marshall Ids and Tonga); Pseudochromis aldabraensis Bauchot-Boutin, 1958 (Aldabra, northwestern Indian Ocean); P. alticaudex sp. nov. (northeastern Indonesia to Solomon Ids); P. andamanensis Lubbock, 1980 (Andaman Sea to Timor Sea, Australia); P. aureolineatus sp. nov. (Comoro Ids); P. aurulentus Gill & Randall, 1998 (Komodo Id, Indonesia); P. bitaeniatus (Fowler, 1931) (Philippines to Timor Sea, Australia, Solomon Ids); P. caudalis Boulenger, 1898 (Arabian Sea to Sri Lanka); P. coccinicauda (Tickell, 1888) (Laccadive Ids to central Indonesia); P. colei Herre, 1933 (Culion, Philippines); P. cometes Gill & Randall, 1998 (Komodo Id, Indonesia); P. cyanotaenia Bleeker, 1857 (Japan to Australia and Vanuatu); P dilectus Lubbock, 1976 (Sri Lanka); P. dixurus Lubbock, 1975 (Red Sea); P. dutoiti Smith, 1955 (east coast of Africa); P. elongatus Lubbock, 1980 (eastern Indonesia); P. flammicauda Lubbock & Goldman, 1976 (Great Barrier Reef); P. flavivertex Riippell, 1835 (Red Sea); P. flavopunctatus Gill & Randall, 1998 (Komodo Id, Indonesia); P. fowleri Herre, 1934 (Philippines and Sabah, Borneo); P. fridmani Klausewitz, 1968 (Red Sea); P. fuscus Muller & Troschel, 1849 (Sri Lanka to Vanuatu); P. howsoni Allen, 1995 (northwestern Australia); P. jamesi Schultz, 1943 (southwest Pacific); P kolythrus Gill & Winterbottom, 1993 (New Caledonia); P. kristinae sp. nov. (east coast of Africa to Madagascar); P. leucorhynchus Lubbock, 1977 (Kenya to Oman); P. linda Randall & Stanaland, 1989 (Gulf of Aden to Pakistan); P. litus Gill & Randall, 1998 (southeastern Indonesia); P. luteus Aoyagi, 1943 (Ryukyu Ids to Philippines); P. madagascariensis sp. nov. (northeastern Madagascar); P. magnificus Lubbock, 1977 (Cargados Carajos Shoals); P. marshallensis Schultz, 1953 (Western Australia to Marshall Ids); P. melanurus sp. nov. (Fiji and Tonga); P. melas Lubbock, 1977 (east coast of Africa); P. mooii sp. nov. (Komodo Id, Indonesia); P. moorei Fowler, 1931 (Philippines); P. natalensis Regan, 1916 (east coast of Africa); P. nigrovittatus Boulenger, 1897 (Red Sea, Gulf of Aden, Socotra to Persian Gulf); P- olivaceus Riippell, 1835 (Red Sea); P. omanensis Gill & Mee, 1993 (Oman); P. persicus Murray, 1887 (Persian Gulf to Pakistan); P. perspicillatus Gunther, 1862 (Philippines and Indonesia); P. pesi Lubbock, 1975 (Red Sea); P. pictus Gill & Randall, 1998 (Alor Id, Indonesia); P. punctatus Kotthaus, 1970 (Somalia and southern Oman); P. pylei Randall & McCosker, 1989 (southeastern Indonesia and Belau); P quinquedentatus McCulloch, 1926 (northern Australia); P. ransonneti Steindachner, 1870 (Gulf of Thailand to Seribu Ids, Indonesia); P. reticulatus Gill & Woodland, 1992 (northwestern Australia); P. sankeyi Lubbock, 1975 (southern Red Sea, Gulf of Aden); P. springeri Lubbock, 1975 (Red Sea); P. steenei Gill & Randall, 1992 (southern Indonesia); P. striatus Gill, Shao & Chen, 1995 (Philippines, Taiwan, Ryukyu Ids); P. tapeinosoma Bleeker, 1853 (Andaman Sea to Solomon Ids); P. tauberae Lubbock, 1977 (east coast of Africa to Madagascar); P. viridis Gill & Allen, 1996 (Christmas Id, Indian Ocean); and P. wilsoni Whitley, 1929 (northern Australia). A key to genera and keys to species within genera are provided. Synonymy lists, suggested vernacular names, morphological descriptions, habitat notes, and distribution maps are given for each species. Photographs showing live and/or freshly dead colourations (including sexual and other intraspecific variation) are provided for all but a few species.
- Full Text:
- Date Issued: 2004
- Authors: Gill, Anthony C
- Date: 2004
- Subjects: Fishes -- Indo-Pacific Region -- Classification , Pseudochromidae
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/137669 , vital:37548
- Description: The 100 nominal species in the pseudochromid subfamily Pseudochrominae are referred to 70 valid species, and an additional ten species are described as new. These species are assigned to ten genera: Assiculoides Gill & Hutchins, 1997, Assiculus Richardson, 1846, Cypho Myers, 1940, Labracinns Schlegel, 1858, Ogilbyina Fowler, 1931, Pseudochromis Riippell, 1835, and four new genera, Manonichthvs, Oxvcercichthys, Pholidochromis and Pictichromis. In previous recent studies only two genera, Labracinns and Pseudochromis , had been generally recognised; species assigned to the remaining genera had been previously placed in Pseudochromis. Species included in the subfamily are: Assiculoides desmonotus Gill & Hutchins, 1997 (Western Australia); Assiculus punctatus Richardson, 1846 (northwestern Australia); Cypho purpurascens (De Vis, 1884) (southwest Pacific); C. zaps sp. nov. (Indonesia to Ryukyu Ids); Labracinns atrofasciatus (Herre, 1933) (Culion, Philippines); L. cyclophthalmus (Muller & Troschel, 1849) (Japan to northwestern Australia, Papua New Guinea); L. lineatus (Castelnau, 1875) (Western Australia); Manonichthvs a/leni sp. nov. (Sabah, Borneo); M. paranox (Lubbock & Goldman, 1976) (Solomon Ids, Papua New Guinea, Great Barrier Reef); M. polynemus (Fowler, 1931) (northeastern Indonesia, Belau); M. splendens (Fowler, 1931) (southeastern Indonesia); M. winterbottomi sp nov. (Cebu, Philippines); Ogilbyina novaehollandiae (Steindachner, 1880) (southern Great Barrier Reef and Queensland); O. queenslandiae (Saville-Kent, 1893) (Queensland, Great Barrier Reef); O. salvati (Plessis & Fourmanoir, 1966) (New Caledonia); Oxvcercichthys veliferus (Lubbock, 1980) (Great Barrier Reef, western Coral Sea); Pholidochromis marginata (Lubbock, 1980) (northeastern Indonesia to Bougainville); Pictichromis aurifrons (Lubbock, 1980) (New Guinea); P. coralensis sp. nov. (Great Barrier Reef to New Caledonia); P. diadema (Lubbock & Randall, 1978) (Malaysia to Philippines and northern Borneo); P. ephippiata (Gill, Pyle & Earle, 1996) (northern Sulawesi, southeastern Papua New Guinea); P. paccagnellae (Axelrod, 1973) (Indonesia, Timor Sea to Solomon Ids); P. porphyrea (Lubbock & Goldman, 1974) (Ryukyu Ids and northeastern Indonesia to Marshall Ids and Tonga); Pseudochromis aldabraensis Bauchot-Boutin, 1958 (Aldabra, northwestern Indian Ocean); P. alticaudex sp. nov. (northeastern Indonesia to Solomon Ids); P. andamanensis Lubbock, 1980 (Andaman Sea to Timor Sea, Australia); P. aureolineatus sp. nov. (Comoro Ids); P. aurulentus Gill & Randall, 1998 (Komodo Id, Indonesia); P. bitaeniatus (Fowler, 1931) (Philippines to Timor Sea, Australia, Solomon Ids); P. caudalis Boulenger, 1898 (Arabian Sea to Sri Lanka); P. coccinicauda (Tickell, 1888) (Laccadive Ids to central Indonesia); P. colei Herre, 1933 (Culion, Philippines); P. cometes Gill & Randall, 1998 (Komodo Id, Indonesia); P. cyanotaenia Bleeker, 1857 (Japan to Australia and Vanuatu); P dilectus Lubbock, 1976 (Sri Lanka); P. dixurus Lubbock, 1975 (Red Sea); P. dutoiti Smith, 1955 (east coast of Africa); P. elongatus Lubbock, 1980 (eastern Indonesia); P. flammicauda Lubbock & Goldman, 1976 (Great Barrier Reef); P. flavivertex Riippell, 1835 (Red Sea); P. flavopunctatus Gill & Randall, 1998 (Komodo Id, Indonesia); P. fowleri Herre, 1934 (Philippines and Sabah, Borneo); P. fridmani Klausewitz, 1968 (Red Sea); P. fuscus Muller & Troschel, 1849 (Sri Lanka to Vanuatu); P. howsoni Allen, 1995 (northwestern Australia); P. jamesi Schultz, 1943 (southwest Pacific); P kolythrus Gill & Winterbottom, 1993 (New Caledonia); P. kristinae sp. nov. (east coast of Africa to Madagascar); P. leucorhynchus Lubbock, 1977 (Kenya to Oman); P. linda Randall & Stanaland, 1989 (Gulf of Aden to Pakistan); P. litus Gill & Randall, 1998 (southeastern Indonesia); P. luteus Aoyagi, 1943 (Ryukyu Ids to Philippines); P. madagascariensis sp. nov. (northeastern Madagascar); P. magnificus Lubbock, 1977 (Cargados Carajos Shoals); P. marshallensis Schultz, 1953 (Western Australia to Marshall Ids); P. melanurus sp. nov. (Fiji and Tonga); P. melas Lubbock, 1977 (east coast of Africa); P. mooii sp. nov. (Komodo Id, Indonesia); P. moorei Fowler, 1931 (Philippines); P. natalensis Regan, 1916 (east coast of Africa); P. nigrovittatus Boulenger, 1897 (Red Sea, Gulf of Aden, Socotra to Persian Gulf); P- olivaceus Riippell, 1835 (Red Sea); P. omanensis Gill & Mee, 1993 (Oman); P. persicus Murray, 1887 (Persian Gulf to Pakistan); P. perspicillatus Gunther, 1862 (Philippines and Indonesia); P. pesi Lubbock, 1975 (Red Sea); P. pictus Gill & Randall, 1998 (Alor Id, Indonesia); P. punctatus Kotthaus, 1970 (Somalia and southern Oman); P. pylei Randall & McCosker, 1989 (southeastern Indonesia and Belau); P quinquedentatus McCulloch, 1926 (northern Australia); P. ransonneti Steindachner, 1870 (Gulf of Thailand to Seribu Ids, Indonesia); P. reticulatus Gill & Woodland, 1992 (northwestern Australia); P. sankeyi Lubbock, 1975 (southern Red Sea, Gulf of Aden); P. springeri Lubbock, 1975 (Red Sea); P. steenei Gill & Randall, 1992 (southern Indonesia); P. striatus Gill, Shao & Chen, 1995 (Philippines, Taiwan, Ryukyu Ids); P. tapeinosoma Bleeker, 1853 (Andaman Sea to Solomon Ids); P. tauberae Lubbock, 1977 (east coast of Africa to Madagascar); P. viridis Gill & Allen, 1996 (Christmas Id, Indian Ocean); and P. wilsoni Whitley, 1929 (northern Australia). A key to genera and keys to species within genera are provided. Synonymy lists, suggested vernacular names, morphological descriptions, habitat notes, and distribution maps are given for each species. Photographs showing live and/or freshly dead colourations (including sexual and other intraspecific variation) are provided for all but a few species.
- Full Text:
- Date Issued: 2004
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26030 , vital:64782
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26030 , vital:64782
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
Modelling of the performance of a batch biogas digester fed with selected types of substrates
- Authors: Mukumba, Patrick
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc (Physics)
- Identifier: vital:11596 , http://hdl.handle.net/10353/d1016197
- Description: The increasing population and the rapid economic growth in South Africa have led to higher consumption of food resulting in the generation of large amounts of waste. In addition, South Africa has plenty of biomass from cattle, donkeys, horses, goats, pigs, chicken and sheep. However, anaerobic digestion could be an alternative solution for the utilization of these kinds of waste due to its environmental and economic benefits. Therefore, the main focus of the research was design, construct a field batch biogas digester, monitor its performance when fed with co-substrates and model the methane yield for an optimized mixing ratio.
- Full Text:
- Date Issued: 2013
- Authors: Mukumba, Patrick
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc (Physics)
- Identifier: vital:11596 , http://hdl.handle.net/10353/d1016197
- Description: The increasing population and the rapid economic growth in South Africa have led to higher consumption of food resulting in the generation of large amounts of waste. In addition, South Africa has plenty of biomass from cattle, donkeys, horses, goats, pigs, chicken and sheep. However, anaerobic digestion could be an alternative solution for the utilization of these kinds of waste due to its environmental and economic benefits. Therefore, the main focus of the research was design, construct a field batch biogas digester, monitor its performance when fed with co-substrates and model the methane yield for an optimized mixing ratio.
- Full Text:
- Date Issued: 2013
Comparative performance of natural and synthetic fibre nonwoven geotextiles
- Tshifularo, Cyrus Alushavhiwi
- Authors: Tshifularo, Cyrus Alushavhiwi
- Date: 2017
- Subjects: Geotextiles Textile fabrics , Textile fibers -- Testing Textile industry -- Quality control
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21362 , vital:29504
- Description: The aim of this work was to establish a range of suitable process parameters which can be utilized to produce needlepunched nonwoven fabrics for geotextile applications. Nonwoven fabrics were produced from 100% PP, a blend of 50/50% PP/kenaf and 100% kenaf fibres. The depths of needle penetration of 4, 7 and 10 mm, stroke frequencies of 250, 350 and 450 strokes/min and mass per unit area of 300, 600 and 900 g/m2 were utilized for producing the fabrics, on a Dilo loom. The effect of depth of needle penetration, stroke frequency and mass per unit area on the fabric properties, namely, tensile strength, puncture resistance, pore size, water permeability and transmissivity were analysed. In addition, the effect of chemicals, namely, 10% ammonium hydroxide (NH4OH), 10% sodium chloride (NaCl) and 3% sulphuric acid (H2SO4) solutions on degradation of the fabric was also studied. The results have shown that density, thickness and nominal weight of the needlepunched nonwoven fabrics were related to each other and they were influenced by stroke frequency, depth of needle penetration and feed rate of the needlepunching process. The increase in nominal weight of the fabrics also increases thickness and density of the fabrics. The tensile strength and puncture resistance of the fabrics increased with the increases in stroke frequency, depth of needle penetration and fabric mass per unit area. However, lower tensile strength and puncture resistance were achieved in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area. Bigger pores were resulted in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area, however, pore size decreased with increases in stroke frequency, depth of needle penetration and mass per unit area. Water permeability depends on the pore size, properties of the fibres, stroke frequency, depth of needle penetration and mass per unit area. Higher tensile strength and higher puncture resistance were achieved in the needlepunched nonwoven fabrics produced from 100% PP fibres, therefore, they are suitable for some load-bearing geotextile applications, such as reinforcement and separation. However, higher water permeability was achieved in the fabrics produced from 100% kenaf fibres, therefore, they are ideal for geotextile applications where good water permeability is required. Higher values for transmissivity were obtained in the fabrics produced from a blend of 50/50% PP/kenaf fibres, therefore they are suitable for drainage applications. The fabrics produced from a blend of 50/50% PP/kenaf fibres achieved better values of tensile strength, puncture resistance, pore size and water permeability in comparison to that produced from 100% PP and 100% kenaf fibres. However, better tensile strength and puncture resistance were achieved in the fabrics produced from 100% PP fibres and bigger pore size and higher water permeability were achieved in the fabrics produced from 100% kenaf fibres. Therefore, it can be suggested that the nonwoven fabrics produced from a blend of 50/50% PP/kenaf fibres can fulfil almost all requirements of geotextile applications, such as, filtration, separation, reinforcement and drainage. The fabrics produced from 100% PP fibres were not damaged or deteriorated when treated with all the three chemicals due to chemical inertness of polypropylene. However, the fabrics produced from a blend of 50/50% PP/kenaf and 100% kenaf fibres were damaged and deteriorated when treated with H2SO4.
- Full Text:
- Date Issued: 2017
- Authors: Tshifularo, Cyrus Alushavhiwi
- Date: 2017
- Subjects: Geotextiles Textile fabrics , Textile fibers -- Testing Textile industry -- Quality control
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21362 , vital:29504
- Description: The aim of this work was to establish a range of suitable process parameters which can be utilized to produce needlepunched nonwoven fabrics for geotextile applications. Nonwoven fabrics were produced from 100% PP, a blend of 50/50% PP/kenaf and 100% kenaf fibres. The depths of needle penetration of 4, 7 and 10 mm, stroke frequencies of 250, 350 and 450 strokes/min and mass per unit area of 300, 600 and 900 g/m2 were utilized for producing the fabrics, on a Dilo loom. The effect of depth of needle penetration, stroke frequency and mass per unit area on the fabric properties, namely, tensile strength, puncture resistance, pore size, water permeability and transmissivity were analysed. In addition, the effect of chemicals, namely, 10% ammonium hydroxide (NH4OH), 10% sodium chloride (NaCl) and 3% sulphuric acid (H2SO4) solutions on degradation of the fabric was also studied. The results have shown that density, thickness and nominal weight of the needlepunched nonwoven fabrics were related to each other and they were influenced by stroke frequency, depth of needle penetration and feed rate of the needlepunching process. The increase in nominal weight of the fabrics also increases thickness and density of the fabrics. The tensile strength and puncture resistance of the fabrics increased with the increases in stroke frequency, depth of needle penetration and fabric mass per unit area. However, lower tensile strength and puncture resistance were achieved in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area. Bigger pores were resulted in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area, however, pore size decreased with increases in stroke frequency, depth of needle penetration and mass per unit area. Water permeability depends on the pore size, properties of the fibres, stroke frequency, depth of needle penetration and mass per unit area. Higher tensile strength and higher puncture resistance were achieved in the needlepunched nonwoven fabrics produced from 100% PP fibres, therefore, they are suitable for some load-bearing geotextile applications, such as reinforcement and separation. However, higher water permeability was achieved in the fabrics produced from 100% kenaf fibres, therefore, they are ideal for geotextile applications where good water permeability is required. Higher values for transmissivity were obtained in the fabrics produced from a blend of 50/50% PP/kenaf fibres, therefore they are suitable for drainage applications. The fabrics produced from a blend of 50/50% PP/kenaf fibres achieved better values of tensile strength, puncture resistance, pore size and water permeability in comparison to that produced from 100% PP and 100% kenaf fibres. However, better tensile strength and puncture resistance were achieved in the fabrics produced from 100% PP fibres and bigger pore size and higher water permeability were achieved in the fabrics produced from 100% kenaf fibres. Therefore, it can be suggested that the nonwoven fabrics produced from a blend of 50/50% PP/kenaf fibres can fulfil almost all requirements of geotextile applications, such as, filtration, separation, reinforcement and drainage. The fabrics produced from 100% PP fibres were not damaged or deteriorated when treated with all the three chemicals due to chemical inertness of polypropylene. However, the fabrics produced from a blend of 50/50% PP/kenaf and 100% kenaf fibres were damaged and deteriorated when treated with H2SO4.
- Full Text:
- Date Issued: 2017
Production and characterization of a bioflocculant from a consortium of bacteria belonging to the halomonas and micrococcus genera
- Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculents , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/25822 , vital:64489
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculents , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/25822 , vital:64489
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
Production and characterization of a bioflocculant from a consortium of bacteria belonging to the halomonas and micrococcus genera.
- Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculants , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26119 , vital:64917
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Faculty Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculants , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26119 , vital:64917
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Faculty Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
Cloning, expression, partial characterisation and application of a recombinant GH10 xylanase, XT6, from Geobacillus stearothermophilus T6 as an additive to chicken feeds
- Authors: Sithole, Tariro
- Date: 2022-04-06
- Subjects: Chicken feed industry , Chickens Feeding and feeds , Bacillus (Bacteria) , Xylanases , Polysaccharides , Geobacillus stearothermophilus
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/292693 , vital:57007
- Description: Monogastric animal farming has largely been sustained by feeding animals with grain feedstocks containing non-starch polysaccharides (NSPs) and anti-nutritive factors, which cause adverse effects, such as increased digesta viscosity and entrapment of nutrients, which leads to the inaccessibility of nutrients. These effects have been linked to a reduction in nutrient digestion and absorption, which results in a decreased feed conversion ratio, energy metabolism and animal growth. Monogastric animals do not produce enzymes that can hydrolyse these NSPs. The application of exogenous enzymes as supplements to animal feeds has been implemented to reduce viscosity and increase nutrient absorption in poultry and pigs over the past few decades. The aim of this study was to clone, express, partially characterise and apply a glycoside hydrolase (GH) family 10 xylanase (XT6), derived from Geobacillus stearothermophilus T6, as an additive to locally produced chicken feeds. The xt6 gene (1,236 bp) was subcloned and expressed in Escherichia coli DH5α and BL21(DE3) cells, respectively. Upon expression, XT6 had a molecular weight of 42 kDa and was partially purified by Ni-NTA chromatography and ultrafiltration. The purification step resulted in a yield of 66.7% with a 16.8-fold increase in purification. XT6 exhibited maximal activity when incubated at a pH and temperature of pH 6.0 and 70°C, respectively, with a high thermostability over a broad range of pH (2–9) and temperature (30–90 °C). The specific activities of XT6 on extracted soluble and insoluble wheat flour arabinoxylans were 110.9 U/mg and 63.98 U/mg, respectively. Kinetic data showed that XT6 displayed a higher catalytic activity and affinity (Vmax = 231.60 μmol/min/mg and KM = 2.759 mg/ml) for soluble wheat arabinoxylan, compared to insoluble wheat arabinoxylan (Vmax = 99.02 μmol/min/mg and KM = 5.058 mg/ml). High-performance liquid chromatography (HPLC) analysis showed that the enzyme hydrolysed wheat flour, arabinoxylan and chicken feeds, producing a range of xylooligosaccharides (XOS), with xylotetraose and xylopentaose being the predominant XOS species. Hydrolysis of both soluble and insoluble wheat flour arabinoxylans by XT6 led to a significant reduction in substrate viscosity. The effects of simulated gastrointestinal fluid contents, such as proteases, bile salts and mucins, on XT6 stability were also studied. Exposure of XT6 to pepsin did not significantly reduce its activity; however, the inhibitory effect of trypsin and mucin on XT6 was much greater. The presence of gut-derived bile salts had no iii | P a g e significant effect on XT6 activity. Finally, it was shown that the XOS produced from the hydrolysis of chicken feeds (starter and grower feeds) by XT6 significantly enhanced the growth of the probiotic bacteria B. subtilis, while there was no significant improvement in the growth of S. thermophilus and L. bulgaricus. In conclusion, the recombinantly produced XT6 demonstrated efficient hydrolysis of starter and grower feeds, and produced XOS that showed prebiotic activity on selected probiotic bacteria. In addition, the pH, temperature and simulated gastric juice content stability of XT6 renders it an attractive candidate as an additive for chicken feeds. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-04-06
- Authors: Sithole, Tariro
- Date: 2022-04-06
- Subjects: Chicken feed industry , Chickens Feeding and feeds , Bacillus (Bacteria) , Xylanases , Polysaccharides , Geobacillus stearothermophilus
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/292693 , vital:57007
- Description: Monogastric animal farming has largely been sustained by feeding animals with grain feedstocks containing non-starch polysaccharides (NSPs) and anti-nutritive factors, which cause adverse effects, such as increased digesta viscosity and entrapment of nutrients, which leads to the inaccessibility of nutrients. These effects have been linked to a reduction in nutrient digestion and absorption, which results in a decreased feed conversion ratio, energy metabolism and animal growth. Monogastric animals do not produce enzymes that can hydrolyse these NSPs. The application of exogenous enzymes as supplements to animal feeds has been implemented to reduce viscosity and increase nutrient absorption in poultry and pigs over the past few decades. The aim of this study was to clone, express, partially characterise and apply a glycoside hydrolase (GH) family 10 xylanase (XT6), derived from Geobacillus stearothermophilus T6, as an additive to locally produced chicken feeds. The xt6 gene (1,236 bp) was subcloned and expressed in Escherichia coli DH5α and BL21(DE3) cells, respectively. Upon expression, XT6 had a molecular weight of 42 kDa and was partially purified by Ni-NTA chromatography and ultrafiltration. The purification step resulted in a yield of 66.7% with a 16.8-fold increase in purification. XT6 exhibited maximal activity when incubated at a pH and temperature of pH 6.0 and 70°C, respectively, with a high thermostability over a broad range of pH (2–9) and temperature (30–90 °C). The specific activities of XT6 on extracted soluble and insoluble wheat flour arabinoxylans were 110.9 U/mg and 63.98 U/mg, respectively. Kinetic data showed that XT6 displayed a higher catalytic activity and affinity (Vmax = 231.60 μmol/min/mg and KM = 2.759 mg/ml) for soluble wheat arabinoxylan, compared to insoluble wheat arabinoxylan (Vmax = 99.02 μmol/min/mg and KM = 5.058 mg/ml). High-performance liquid chromatography (HPLC) analysis showed that the enzyme hydrolysed wheat flour, arabinoxylan and chicken feeds, producing a range of xylooligosaccharides (XOS), with xylotetraose and xylopentaose being the predominant XOS species. Hydrolysis of both soluble and insoluble wheat flour arabinoxylans by XT6 led to a significant reduction in substrate viscosity. The effects of simulated gastrointestinal fluid contents, such as proteases, bile salts and mucins, on XT6 stability were also studied. Exposure of XT6 to pepsin did not significantly reduce its activity; however, the inhibitory effect of trypsin and mucin on XT6 was much greater. The presence of gut-derived bile salts had no iii | P a g e significant effect on XT6 activity. Finally, it was shown that the XOS produced from the hydrolysis of chicken feeds (starter and grower feeds) by XT6 significantly enhanced the growth of the probiotic bacteria B. subtilis, while there was no significant improvement in the growth of S. thermophilus and L. bulgaricus. In conclusion, the recombinantly produced XT6 demonstrated efficient hydrolysis of starter and grower feeds, and produced XOS that showed prebiotic activity on selected probiotic bacteria. In addition, the pH, temperature and simulated gastric juice content stability of XT6 renders it an attractive candidate as an additive for chicken feeds. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-04-06