- Title
- Analysis of Discordance between Genotypic and Phenotypic Assays for Rifampicin-Resistant Mycobacterium tuberculosis Isolated from Healthcare Facilities in Mthatha
- Creator
- Bokop, Carine
- Creator
- Faye, M Lindiwe
- Creator
- Apalata, Teke
- Subject
- Xpert MTB/RIF
- Subject
- Line probe assay
- Subject
- MGIT 960 system
- Subject
- Mycobacterium tuburculosis
- Subject
- Discrepant results
- Date Issued
- 2023/07
- Date
- 2023/07
- Type
- Article
- Identifier
- http://hdl.handle.net/11260/13652
- Identifier
- vital:78963
- Identifier
- DOI: https://doi.org/10.3390/pathogens12070909
- Description
- The study sought to determine the rate of discordant results between genotypic and phenotypic tests for the diagnosis of drug-resistant tuberculosis (DR-TB). Sputum samples and cultured isolates from suspected DR-TB patients were, respectively, analyzed for Mycobacterium tuberculosis by Xpert® MTB/RIF (Cepheid, Sunnyvale, CA, USA) and line probe assays (LPA) (Hain, Nehren, Germany). Discrepant rifampicin (RMP)-resistant results were confirmed using BACTEC MGIT960 (BD, New York, NY, USA). Of the 224 RMP-resistant results obtained by Xpert MTB/RIF, 5.4% were susceptible to RMP by LPA. MGIT960 showed a 75% agreement with LPA. The discrepancy was attributed to either heteroresistance or DNA contamination during LPA testing in 58.3% of cases. In 25% of the samples showing agreement in RMP resistance between Xpert MTB/RIF and MGIT960, the discrepancy was attributed to laboratory errors causing false RMP susceptible results with LPA. In 16.7% of the cases, the discrepancy was attributed to false RMP susceptible results with Xpert MTB/RIF. Out of the 224 isolates, susceptibility to isoniazid (INH) by LPA was performed in 73.7% RMP-resistant isolates, of which, 80.6% were resistant. All RMP-resistant isolates by Xpert MTB/RIF were confirmed in 98.5% by LPA if TB isolates were resistant to INH, but were only confirmed in 81.3% if TB isolates were susceptible to INH....
- Language
- English
- Relation
- pathogens
- Relation
- Bokop, C.; Faye, L.M.; Apalata, T. Analysis of Discordance between Genotypic and Phenotypic Assays for Rifampicin-Resistant Mycobacterium tuberculosis Isolated from Healthcare Facilities in Mthatha. Pathogens 2023, 12, 909. https:// doi.org/10.3390/pathogens12070909
- Relation
- pathogens 12 vol 7 1 12 2023 https://doi.org/10.3390/pathogens12070909
- Rights
- ©2023, authors
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