Metal bioaccumulation and precious metal refinery wastewater treatment by phoma glomerata
- Authors: Moore, Bronwyn Ann
- Date: 2008-03-18
- Subjects: Metals -- Bioaccumulation , Water purification -- South Africa , Metal ions , Water -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Factory and trade waste -- Purification -- South Africa , Metals -- Refining , Hyperaccumulator plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4097 , http://hdl.handle.net/10962/d1009441 , Metals -- Bioaccumulation , Water purification -- South Africa , Metal ions , Water -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Factory and trade waste -- Purification -- South Africa , Metals -- Refining , Hyperaccumulator plants
- Description: The biosorption of copper, nickel, gold and platinum from single metal aqueous solutions by the nickel hyperaccumulator Berkheya coddii plant biomass was investigated. Potentiometric titrations of the biomass and determination of optimal sorption pH for each metal showed that nickel ions were released from the biomass into solution. The presence of free nickel ions interfered with the uptake of the other three metals and further biosorption investigations were discontinued. Three fungal isolates found colonising metal solutions were cultured and screened for their ability to remove 50 mg.l⁻¹ of copper, nickel, gold and platinum from solution and to survive and grow in precious metal refinery wastewaters. One isolate was selected for further studies based on its superior metal uptake capabilities (35 and 39 mg.l⁻¹ of gold and platinum, respectively) and was identified as Phoma glomerata. Copper, nickel, gold and platinum uptake studies revealed that nickel and gold were the most toxic metal ions, however, toxicity was dependent on pH. At pH 6 more biomass growth was achieved than at lower pH values and metal uptake increased by 51 and 17 % for copper and nickel, respectively. In addition, the production of extracellular polymeric substances played a role in base metal interaction. Precious metals were observed to be preferentially removed from solution, complete removal of gold and platinum was observed at all initial pH values, 89 % of copper was bioaccumulated at an initial metal concentration of 55 mg.l⁻¹ (pH 6) and only 23 % of nickel was removed from solution under the same conditions. Metal bioaccumulation was confirmed through transmission electron microscopy and micro particle induced X-ray emission. The effect of P. glomerata immobilised in a packed bed reactor on precious metal refinery wastewaters was investigated. It was found that the fungal isolate was not able to remove the high salt and chemical oxygen demand concentrations found in the wastewaters, however due to its ability to survive and grow in undiluted wastewater and remove metal ions from solution it may be utilised as a metal detoxification step in the treatment process train. , PDFCreator Version 0.9.0 , AFPL Ghostscript 8.53
- Full Text:
- Authors: Moore, Bronwyn Ann
- Date: 2008-03-18
- Subjects: Metals -- Bioaccumulation , Water purification -- South Africa , Metal ions , Water -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Factory and trade waste -- Purification -- South Africa , Metals -- Refining , Hyperaccumulator plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4097 , http://hdl.handle.net/10962/d1009441 , Metals -- Bioaccumulation , Water purification -- South Africa , Metal ions , Water -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Factory and trade waste -- Purification -- South Africa , Metals -- Refining , Hyperaccumulator plants
- Description: The biosorption of copper, nickel, gold and platinum from single metal aqueous solutions by the nickel hyperaccumulator Berkheya coddii plant biomass was investigated. Potentiometric titrations of the biomass and determination of optimal sorption pH for each metal showed that nickel ions were released from the biomass into solution. The presence of free nickel ions interfered with the uptake of the other three metals and further biosorption investigations were discontinued. Three fungal isolates found colonising metal solutions were cultured and screened for their ability to remove 50 mg.l⁻¹ of copper, nickel, gold and platinum from solution and to survive and grow in precious metal refinery wastewaters. One isolate was selected for further studies based on its superior metal uptake capabilities (35 and 39 mg.l⁻¹ of gold and platinum, respectively) and was identified as Phoma glomerata. Copper, nickel, gold and platinum uptake studies revealed that nickel and gold were the most toxic metal ions, however, toxicity was dependent on pH. At pH 6 more biomass growth was achieved than at lower pH values and metal uptake increased by 51 and 17 % for copper and nickel, respectively. In addition, the production of extracellular polymeric substances played a role in base metal interaction. Precious metals were observed to be preferentially removed from solution, complete removal of gold and platinum was observed at all initial pH values, 89 % of copper was bioaccumulated at an initial metal concentration of 55 mg.l⁻¹ (pH 6) and only 23 % of nickel was removed from solution under the same conditions. Metal bioaccumulation was confirmed through transmission electron microscopy and micro particle induced X-ray emission. The effect of P. glomerata immobilised in a packed bed reactor on precious metal refinery wastewaters was investigated. It was found that the fungal isolate was not able to remove the high salt and chemical oxygen demand concentrations found in the wastewaters, however due to its ability to survive and grow in undiluted wastewater and remove metal ions from solution it may be utilised as a metal detoxification step in the treatment process train. , PDFCreator Version 0.9.0 , AFPL Ghostscript 8.53
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An investigation into the use of anaerobic digestion for the treatment of tannery wastewaters
- Authors: Jackson-Moss, Clive Alan
- Date: 1991
- Subjects: Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4059 , http://hdl.handle.net/10962/d1004120 , Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Description: The anaerobic digestion of tannery wastewaters was investigated with a view to using this form of treatment in the tanning industry. As these wastewaters are extremely complex and contain high concentrations of both inorganic and organic compounds, the effect of these individual compounds on the anaerobic digestion process was investigated in detail, in order to ascertain the fate of these compounds during the digestion process. The experiments comprising the initial toxicity study were carried out as adaptation experiments using a synthetic wastewater. It was found that the heavy metals such as chrome, aluminium and iron precipitated and accumulated in the sludge bed of the digesters . The soluble ions such as sodium and chloride were not retained and passed through the digesters. Approximately 20 % of the calcium ions were removed through precipitation, with the remainder being present in the digester effluent . Under the anaerobic conditions, ammonification of the organic nitrogen occurred, and influent sulphates were reduced to sulphides . These sulphides were present as either H2S, HS or insoluble sulphides. As these compounds under investigation on caused no inhibition of the anaerobic digestion process at the concentrations found in tannery wastewaters, the anaerobic treatment of these wastewaters appeared to be possible, provided the bacteria were given sufficient time to adapt to the potentially toxic compounds. However, despite the findings of the synthetic study, the successful anaerobic digestion of the tannery effluents could not be achieved. Although the use of acid was found to be essential in order to control the digester pH in the optimum range, the metabolism of the methanogenic bacteria was inhibited by the presence or absence of unknown compounds. Neither the addition of essential trace nutrients, nor the prevention of the competition between the methanogens and the sulphate-reducing bacteria were able to reverse this inhibition. As tannery effluents contain very low concentrations of phosphorous, it is possible that the methanogens were inhibited by a lack of phosphorous, which is essential during methanogenesis. In contrast to the results obtained from the effluent experiments, the anaerobic digestion of tannery sludge was found to be possible. Of the organic solids present in the sludge, 60 % were degraded and converted into biogas, which had a methane content greater than 70 %. The degradation of the organic solids ensured that COD and PV reductions of greater than 90 % were achieved, and the fate of the compounds in the digesters were in agreement with the findings of the v synthetic study. Efforts to improve the efficiency of the digestion process through the addition of trace nutrients and the use of a two-stage process were only successful in bringing about a minor improvement in digester performance. The overall results of this investigation show, therefore, that although the anaerobic treatment of the tannery effluent was not achieved, the successful anaerobic digestion of tannery sludge is possible at low loading rates. As many difficulties still need to be solved, a great deal of further research is necessary if anaerobic digestion is to be used on an industrial scale for the treatment and disposal of tannery wastewaters.
- Full Text:
- Date Issued: 1991
- Authors: Jackson-Moss, Clive Alan
- Date: 1991
- Subjects: Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4059 , http://hdl.handle.net/10962/d1004120 , Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purification -- Biological treatment -- South Africa , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Description: The anaerobic digestion of tannery wastewaters was investigated with a view to using this form of treatment in the tanning industry. As these wastewaters are extremely complex and contain high concentrations of both inorganic and organic compounds, the effect of these individual compounds on the anaerobic digestion process was investigated in detail, in order to ascertain the fate of these compounds during the digestion process. The experiments comprising the initial toxicity study were carried out as adaptation experiments using a synthetic wastewater. It was found that the heavy metals such as chrome, aluminium and iron precipitated and accumulated in the sludge bed of the digesters . The soluble ions such as sodium and chloride were not retained and passed through the digesters. Approximately 20 % of the calcium ions were removed through precipitation, with the remainder being present in the digester effluent . Under the anaerobic conditions, ammonification of the organic nitrogen occurred, and influent sulphates were reduced to sulphides . These sulphides were present as either H2S, HS or insoluble sulphides. As these compounds under investigation on caused no inhibition of the anaerobic digestion process at the concentrations found in tannery wastewaters, the anaerobic treatment of these wastewaters appeared to be possible, provided the bacteria were given sufficient time to adapt to the potentially toxic compounds. However, despite the findings of the synthetic study, the successful anaerobic digestion of the tannery effluents could not be achieved. Although the use of acid was found to be essential in order to control the digester pH in the optimum range, the metabolism of the methanogenic bacteria was inhibited by the presence or absence of unknown compounds. Neither the addition of essential trace nutrients, nor the prevention of the competition between the methanogens and the sulphate-reducing bacteria were able to reverse this inhibition. As tannery effluents contain very low concentrations of phosphorous, it is possible that the methanogens were inhibited by a lack of phosphorous, which is essential during methanogenesis. In contrast to the results obtained from the effluent experiments, the anaerobic digestion of tannery sludge was found to be possible. Of the organic solids present in the sludge, 60 % were degraded and converted into biogas, which had a methane content greater than 70 %. The degradation of the organic solids ensured that COD and PV reductions of greater than 90 % were achieved, and the fate of the compounds in the digesters were in agreement with the findings of the v synthetic study. Efforts to improve the efficiency of the digestion process through the addition of trace nutrients and the use of a two-stage process were only successful in bringing about a minor improvement in digester performance. The overall results of this investigation show, therefore, that although the anaerobic treatment of the tannery effluent was not achieved, the successful anaerobic digestion of tannery sludge is possible at low loading rates. As many difficulties still need to be solved, a great deal of further research is necessary if anaerobic digestion is to be used on an industrial scale for the treatment and disposal of tannery wastewaters.
- Full Text:
- Date Issued: 1991
Studies on the completely mixed activated sludge treatment of fellmongery and tannery lime-sulphide effluents
- Authors: Rawlings, Douglas Eric
- Date: 1977
- Subjects: Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purificiation -- Biological treatment , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4112 , http://hdl.handle.net/10962/d1013052
- Description: Industries producing highly polluted waste waters are having to purify their effluents to meet with ever increasing requirements laid down by water authorities. The South African Water Act of 1956 has prescribed a very high standard to which waste waters must conform before discharge into a South African water course. Enforcement of these standards falls under the jurisdiction of government authorities such as the Department of Water Affairs. Similarly, municipalities and other local authorities set standards with which trade effluents must comply before discharge into public sewers for treatment in a municipal sewage works. These local authorities are empowered to recover from the trader the additional costs incurred in treating trade effluents. Costs are usually levied in respect of volume, oxygen demand, settleable solids and the production of secondary sludge. In recent years, these standards have been enforced to an extent where the survival of several industries has become dependant on whether these industries are able to purify or dispose of their effluents in a manner acceptable to the water authorities. Chap. 1, p. 1.
- Full Text:
- Date Issued: 1977
- Authors: Rawlings, Douglas Eric
- Date: 1977
- Subjects: Tanneries -- Waste disposal , Sewage sludge -- South Africa -- Management , Sewage -- Purificiation -- Biological treatment , Water quality management -- South Africa , Water -- Purification -- Biological treatment -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4112 , http://hdl.handle.net/10962/d1013052
- Description: Industries producing highly polluted waste waters are having to purify their effluents to meet with ever increasing requirements laid down by water authorities. The South African Water Act of 1956 has prescribed a very high standard to which waste waters must conform before discharge into a South African water course. Enforcement of these standards falls under the jurisdiction of government authorities such as the Department of Water Affairs. Similarly, municipalities and other local authorities set standards with which trade effluents must comply before discharge into public sewers for treatment in a municipal sewage works. These local authorities are empowered to recover from the trader the additional costs incurred in treating trade effluents. Costs are usually levied in respect of volume, oxygen demand, settleable solids and the production of secondary sludge. In recent years, these standards have been enforced to an extent where the survival of several industries has become dependant on whether these industries are able to purify or dispose of their effluents in a manner acceptable to the water authorities. Chap. 1, p. 1.
- Full Text:
- Date Issued: 1977
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