Art and investment a study on how investment in art affects the contemporary artist in South Africa
- Authors: Jones, Caroline Elizabeth
- Date: 1995
- Subjects: Art as an investment Art -- Philosophy Art -- South Africa -- Marketing Art -- Collectors and collecting -- South Africa Art dealers -- South Africa
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2404 , http://hdl.handle.net/10962/d1002200
- Full Text:
- Date Issued: 1995
- Authors: Jones, Caroline Elizabeth
- Date: 1995
- Subjects: Art as an investment Art -- Philosophy Art -- South Africa -- Marketing Art -- Collectors and collecting -- South Africa Art dealers -- South Africa
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2404 , http://hdl.handle.net/10962/d1002200
- Full Text:
- Date Issued: 1995
Art marketing and management
- Authors: Anderson, Larna
- Date: 1995
- Subjects: Art -- Marketing Art portfolios Art -- Finance Art -- Economic aspects Community arts projects
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2392 , http://hdl.handle.net/10962/d1002188
- Description: Formal art education equips students with skills to produce artworks. A formal art education may increase the opportunity for employment, however, art-related employment is very limited. Art graduates would be better equipped to market and manage art establishments or their own careers if art education were to be supplemented with basic business skills. Artists who wish to earn unsupplemented incomes from their art should undertake to acquire business acumen. This includes being presentable to the market place in attitude and appearance. It also includes aptitude in art, marketing and management. Role models and non-models of success and failure in business should also be observed. Art graduates should adopt applicable tried and tested business methods. Good marketing is a mix of business activities which identifies and creates consumer needs and wants. Marketing activities involve research, planning, packaging, pricing, promoting and distributing products and services to the public to create exchanges that satisfy individual and organisational objectives. Art products include artworks, frames, art books and art materials. Art-related services include the undertaking of commissions, consulting, teaching, free parking, convenient shopping hours, acceptance of mail or telephone orders, exhibitions, ease of contact, approval facilities, wrapping, delivery, installations (picture hanging), quotations, discounts, credit facilities, guarantees, trade-ins, adjustments and restorations. Good management is a mix of business activities which enables a venture to meet the challenges of supply and demand. There is a blueprint for management competence. The three dimensions of organisational competence are collaboration, commitment and creativity. Self-marketing and management is an expression of an artist's most creative being. It is that which can ensure and sustain recognition and income. Artists, like other competent organisations and entrepreneurs from the private sector, should operate with efficient manufacturing, marketing, management and finance departments. They are also equally important and therefore demand equal attention. Artistic skill together with business acumen should equip the artist to successfully compete in the market place. There are no short-cuts to becoming an artist but there are short-cuts to becoming a known and financially stable artist. Understanding marketing and management could mean the difference between waiting in poverty and frustration for a "lucky break" (which may only happen after an artists's death) and taking control. Success should be perpetuated through continuous effort.
- Full Text:
- Date Issued: 1995
- Authors: Anderson, Larna
- Date: 1995
- Subjects: Art -- Marketing Art portfolios Art -- Finance Art -- Economic aspects Community arts projects
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2392 , http://hdl.handle.net/10962/d1002188
- Description: Formal art education equips students with skills to produce artworks. A formal art education may increase the opportunity for employment, however, art-related employment is very limited. Art graduates would be better equipped to market and manage art establishments or their own careers if art education were to be supplemented with basic business skills. Artists who wish to earn unsupplemented incomes from their art should undertake to acquire business acumen. This includes being presentable to the market place in attitude and appearance. It also includes aptitude in art, marketing and management. Role models and non-models of success and failure in business should also be observed. Art graduates should adopt applicable tried and tested business methods. Good marketing is a mix of business activities which identifies and creates consumer needs and wants. Marketing activities involve research, planning, packaging, pricing, promoting and distributing products and services to the public to create exchanges that satisfy individual and organisational objectives. Art products include artworks, frames, art books and art materials. Art-related services include the undertaking of commissions, consulting, teaching, free parking, convenient shopping hours, acceptance of mail or telephone orders, exhibitions, ease of contact, approval facilities, wrapping, delivery, installations (picture hanging), quotations, discounts, credit facilities, guarantees, trade-ins, adjustments and restorations. Good management is a mix of business activities which enables a venture to meet the challenges of supply and demand. There is a blueprint for management competence. The three dimensions of organisational competence are collaboration, commitment and creativity. Self-marketing and management is an expression of an artist's most creative being. It is that which can ensure and sustain recognition and income. Artists, like other competent organisations and entrepreneurs from the private sector, should operate with efficient manufacturing, marketing, management and finance departments. They are also equally important and therefore demand equal attention. Artistic skill together with business acumen should equip the artist to successfully compete in the market place. There are no short-cuts to becoming an artist but there are short-cuts to becoming a known and financially stable artist. Understanding marketing and management could mean the difference between waiting in poverty and frustration for a "lucky break" (which may only happen after an artists's death) and taking control. Success should be perpetuated through continuous effort.
- Full Text:
- Date Issued: 1995
Characterisation of the genome of Nudaurelia Omega Virus
- Authors: Cox, Dermot
- Date: 1995
- Subjects: Imbrasia cytherea , RNA , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4023 , http://hdl.handle.net/10962/d1004083 , Imbrasia cytherea , RNA , Insects -- Viruses
- Description: Nudaurelia co virus (Nco V) is a small RNA virus belonging to the Family Tetraviridae. Nco V was successfully isolated from field collected larvae of the pine emperor moth, Nudaurelia cytherea capensis. By polyacrylamide gel electrophoresis-it was possible Jo determine the size of the capsid proteins. Anti-NcoV antiserum was raised by inoculating a rabbit with purified virus. RNA was extracted from the purified virus using a phenol\chloroform extraction procedure. It was possible to separate the viral RNA into its constituent species using sucrose density gradient centrifugation. The sizes of both species of RNA was accurately determined by agarose gel electrophoresis. These sizes corresponded to the replicative form of the RNA which was extracted from infected host tissue. The absence of a poly(A) tract on the RNA was shown through poly(U) sepharose chromatography. Cell-free translation of the viral RNA elucidated the sizes of proteins encoded in vitro in a rabbit reticulocyte lysate system. Optimal conditions for in vitro translation of Nco V were determined for a range of conditions. Immunoprecipitaion of viral encoded proteins with anti-Nco V antiserum suggested that the putative coat protein of the virus was encoded by RNA 2, as a precursor polypeptide which underwent posttranslational cleavage. Reverse transcription - polymerase chain reaction (RT -PCR) was used to successfully produce a radiolabelled probe which could detect dot-blotted viral RNA. The efficacy of this probe in detecting the presence of Nco V RNA in infected tissue was also tested.
- Full Text:
- Date Issued: 1995
- Authors: Cox, Dermot
- Date: 1995
- Subjects: Imbrasia cytherea , RNA , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4023 , http://hdl.handle.net/10962/d1004083 , Imbrasia cytherea , RNA , Insects -- Viruses
- Description: Nudaurelia co virus (Nco V) is a small RNA virus belonging to the Family Tetraviridae. Nco V was successfully isolated from field collected larvae of the pine emperor moth, Nudaurelia cytherea capensis. By polyacrylamide gel electrophoresis-it was possible Jo determine the size of the capsid proteins. Anti-NcoV antiserum was raised by inoculating a rabbit with purified virus. RNA was extracted from the purified virus using a phenol\chloroform extraction procedure. It was possible to separate the viral RNA into its constituent species using sucrose density gradient centrifugation. The sizes of both species of RNA was accurately determined by agarose gel electrophoresis. These sizes corresponded to the replicative form of the RNA which was extracted from infected host tissue. The absence of a poly(A) tract on the RNA was shown through poly(U) sepharose chromatography. Cell-free translation of the viral RNA elucidated the sizes of proteins encoded in vitro in a rabbit reticulocyte lysate system. Optimal conditions for in vitro translation of Nco V were determined for a range of conditions. Immunoprecipitaion of viral encoded proteins with anti-Nco V antiserum suggested that the putative coat protein of the virus was encoded by RNA 2, as a precursor polypeptide which underwent posttranslational cleavage. Reverse transcription - polymerase chain reaction (RT -PCR) was used to successfully produce a radiolabelled probe which could detect dot-blotted viral RNA. The efficacy of this probe in detecting the presence of Nco V RNA in infected tissue was also tested.
- Full Text:
- Date Issued: 1995
Determination of distinctness among citrus cultivars using biochemical and molecular markers
- Authors: Carstens, Karin
- Date: 1995
- Subjects: Citrus fruits -- South Africa , Citrus fruits -- Research -- South Africa , Citrus fruits -- Analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4022 , http://hdl.handle.net/10962/d1004082 , Citrus fruits -- South Africa , Citrus fruits -- Research -- South Africa , Citrus fruits -- Analysis
- Description: Citrus is among the most important fruit crops worlstwide, and therefore the preservation and improvement of citrus germplasm is of the essence. Citrus breeders are often faced with the difficulty of distinguishing between new and existing cultivars because of the ambiguous nature of morphological traits due to environmental influences and error in human judgement. The protection of new varieties is very important to the breeder. New varieties cannot be patented in South Africa, but it can be protected by Plant Breeders' Rights, only if it is genetically distinguishable and significantly different economically from existing varieties. Cultivars in four genera (c. sinensis, C. paradisi, C. grandis and C. reticulata) included in the Citrus Improvement Programme (CIP) or cultivars awaiting recognition of Plant Breeders' Rights by the International Union for the Protection of New Plant Varieties (UPOV) were analyzed with Isoenzymes, Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD). Five enzyme systems (PGM, PGI, MDH, GOT and IDH) were analyzed and founded to be suitable for grouping together cultivars belonging to the same genera. It was not suited for routine discrimination of cultivars in a particular genus. RFLP studies were conducted on five grapefruit cultivars, using cDNA clones from a genomic library of Rough Lemon. RFLP studies were valuable for the discrimination of closely related cultivars which probably originated from a common ancestor by bud mutations. This technique was, however, abandoned due to its biohazardous nature and replaced by the PeR-based Random Amplified Polymorphic DNA. RAPDs are easy to perform and gave promisin& results which were exploited to reveal polymorphisms between cultivars within the various groups. Although the interpretation of data produced by this method is often suspicious, it is the best method currently available for cultivar identification. It can playa complementary role in the protection of new varieties when classical morphological interpretation of differences is not capable of determining sufficient distinctness for the awarding of Plant Breeders' Rights.
- Full Text:
- Date Issued: 1995
- Authors: Carstens, Karin
- Date: 1995
- Subjects: Citrus fruits -- South Africa , Citrus fruits -- Research -- South Africa , Citrus fruits -- Analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4022 , http://hdl.handle.net/10962/d1004082 , Citrus fruits -- South Africa , Citrus fruits -- Research -- South Africa , Citrus fruits -- Analysis
- Description: Citrus is among the most important fruit crops worlstwide, and therefore the preservation and improvement of citrus germplasm is of the essence. Citrus breeders are often faced with the difficulty of distinguishing between new and existing cultivars because of the ambiguous nature of morphological traits due to environmental influences and error in human judgement. The protection of new varieties is very important to the breeder. New varieties cannot be patented in South Africa, but it can be protected by Plant Breeders' Rights, only if it is genetically distinguishable and significantly different economically from existing varieties. Cultivars in four genera (c. sinensis, C. paradisi, C. grandis and C. reticulata) included in the Citrus Improvement Programme (CIP) or cultivars awaiting recognition of Plant Breeders' Rights by the International Union for the Protection of New Plant Varieties (UPOV) were analyzed with Isoenzymes, Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD). Five enzyme systems (PGM, PGI, MDH, GOT and IDH) were analyzed and founded to be suitable for grouping together cultivars belonging to the same genera. It was not suited for routine discrimination of cultivars in a particular genus. RFLP studies were conducted on five grapefruit cultivars, using cDNA clones from a genomic library of Rough Lemon. RFLP studies were valuable for the discrimination of closely related cultivars which probably originated from a common ancestor by bud mutations. This technique was, however, abandoned due to its biohazardous nature and replaced by the PeR-based Random Amplified Polymorphic DNA. RAPDs are easy to perform and gave promisin& results which were exploited to reveal polymorphisms between cultivars within the various groups. Although the interpretation of data produced by this method is often suspicious, it is the best method currently available for cultivar identification. It can playa complementary role in the protection of new varieties when classical morphological interpretation of differences is not capable of determining sufficient distinctness for the awarding of Plant Breeders' Rights.
- Full Text:
- Date Issued: 1995
Determining the relationship between the body image of adolescent girls and their academic achievement, social involvement and sport participation : a pilot study
- Authors: Mostert, Petronella Louisa
- Date: 1995
- Subjects: Body image in adolescence Personality and academic achievement Social interaction in adolescence Self-perception in adolescence Teenage girls -- Psychology Academic achievement
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1492 , http://hdl.handle.net/10962/d1003373
- Description: Ever since the earliest times much emphasis has been placed on the physical appearance of women. Particularly in western cultures idealized images of perfection are constantly promoted and the message that comes across in various media outlets is that a woman ought to have a 'perfect' appearance in order to be competent and desirable. This puts a great deal of stress on women and particularly on adolescent girls who are going through a phase of increased sensitivity about their appearance. The literature suggests that people, and women in particular, are fundamentally affected by the way they view their bodies. The body image furthermore has a strong relationship with the self concept, which affects other areas of a person's life such as her academic achievement and her social 'life. Against this background an attempt was made in this study to determine the relationship between the body image of adolescent girls and their academic achievement, their sport participation and their social involvement. The subjects were 180 girls drawn from two rural secondary schools. The survey involved the completion of two questionnaires, namely an adapted form of the Physical Self Subscale of the Tenessee Self Concept Scale (used to determine a score for body image) and a questionnaire on social involvement and sport participation, which was drawn up by the researcher. Biographic data as well as academic scores were gathered from school records. Data was analysed statistically by means of the BMDP Software Version 1990: programme (Sun Unix). Pearson's chi square tests of independence, as well as a one-way analysis of variance with co-variate were carried out. The results reveal that the body image and academic achievement of this group of girls are not significantly correlated. There are, however, significant correlations between the body image and social involvement of these girls, between their body image and sport participation, as well as between their social involvement and sport participation. iv These findings have important implications for"'e"ducation and consideration is given to addressing these issues in schools.
- Full Text:
- Date Issued: 1995
- Authors: Mostert, Petronella Louisa
- Date: 1995
- Subjects: Body image in adolescence Personality and academic achievement Social interaction in adolescence Self-perception in adolescence Teenage girls -- Psychology Academic achievement
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1492 , http://hdl.handle.net/10962/d1003373
- Description: Ever since the earliest times much emphasis has been placed on the physical appearance of women. Particularly in western cultures idealized images of perfection are constantly promoted and the message that comes across in various media outlets is that a woman ought to have a 'perfect' appearance in order to be competent and desirable. This puts a great deal of stress on women and particularly on adolescent girls who are going through a phase of increased sensitivity about their appearance. The literature suggests that people, and women in particular, are fundamentally affected by the way they view their bodies. The body image furthermore has a strong relationship with the self concept, which affects other areas of a person's life such as her academic achievement and her social 'life. Against this background an attempt was made in this study to determine the relationship between the body image of adolescent girls and their academic achievement, their sport participation and their social involvement. The subjects were 180 girls drawn from two rural secondary schools. The survey involved the completion of two questionnaires, namely an adapted form of the Physical Self Subscale of the Tenessee Self Concept Scale (used to determine a score for body image) and a questionnaire on social involvement and sport participation, which was drawn up by the researcher. Biographic data as well as academic scores were gathered from school records. Data was analysed statistically by means of the BMDP Software Version 1990: programme (Sun Unix). Pearson's chi square tests of independence, as well as a one-way analysis of variance with co-variate were carried out. The results reveal that the body image and academic achievement of this group of girls are not significantly correlated. There are, however, significant correlations between the body image and social involvement of these girls, between their body image and sport participation, as well as between their social involvement and sport participation. iv These findings have important implications for"'e"ducation and consideration is given to addressing these issues in schools.
- Full Text:
- Date Issued: 1995
Disturbing the neighbours: an investigation into the relationship between psychopathology and social formations
- Authors: Collins, Anthony
- Date: 1995
- Subjects: Freud, Sigmund, 1856-1939 , Winnicott, D. W. (Donald Woods), 1896-1971 , Social psychiatry , Social psychology , Psychology, Pathological , Frankfurt school of sociology , Political psychology
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2953 , http://hdl.handle.net/10962/d1002462 , Freud, Sigmund, 1856-1939 , Winnicott, D. W. (Donald Woods), 1896-1971 , Social psychiatry , Social psychology , Psychology, Pathological , Frankfurt school of sociology , Political psychology
- Description: This work attempts to confront certain political problems created by the individualistic bias in psychoanalytic thinking, and the resulting failure to adequately theorise the importance of social processes. The thesis traces the origins of intrapsychic thinking to Freud's initial move from the seduction theory to the Oedipal theory. This development is offered as a prototype for the debates between conceptualisations of childhood traumatisation as a social problem of actual abuse occurring within dermed power relations, and theories which locate pathology purely within internal conflicts occurring inside the individual. Several criticisms of this shift are offered, and it s impact on later theory is considered. Here a contrast is offered between the theoretical approaches of Melanie Klein and Donald Winnicott, arguing for the usefulness of Winnicott's emphasis on environmental factors within psychodynamic theory. The impact of these theoretical approaches is illustrated through a critical evaluation of Freud's case study of Judge Schreber. Additional historical material is brought in to show the importance of environmental considerations ignored by Freud, and contrasting psychodynamic readings of the case are offered. As an alternative to purely intrapsychic approaches, a reinterpretation of certain strands of Critical Theory is then presented. Adorno's theory of the Authoritarian Personality and Marcuse' s concept of One-Dimensional Man are extended using Winnicott's formulations concerning psychological development. This leads to an examination of the question of the relationship between social structure and individual consciousness. Post-structuralist accounts of language and the construction of identity are explored. These are then developed drawing on theories of ideology, language and consciousness, integrating these with Winnicott's developmental theory to offer an alternative psychodynamic understanding of the relationship between social process and psychopathology. An attempt is made to reformulate - the notions of consciousness and the unconscious in terms of the possibilities and difficulties of representation within available social symbolic codes. In conclusion the it is argued that psychology needs to integrate critical social theory and contemporary understandings of the social construction of consciousness in order to become a meaning force in positive social transformation.
- Full Text:
- Date Issued: 1995
- Authors: Collins, Anthony
- Date: 1995
- Subjects: Freud, Sigmund, 1856-1939 , Winnicott, D. W. (Donald Woods), 1896-1971 , Social psychiatry , Social psychology , Psychology, Pathological , Frankfurt school of sociology , Political psychology
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2953 , http://hdl.handle.net/10962/d1002462 , Freud, Sigmund, 1856-1939 , Winnicott, D. W. (Donald Woods), 1896-1971 , Social psychiatry , Social psychology , Psychology, Pathological , Frankfurt school of sociology , Political psychology
- Description: This work attempts to confront certain political problems created by the individualistic bias in psychoanalytic thinking, and the resulting failure to adequately theorise the importance of social processes. The thesis traces the origins of intrapsychic thinking to Freud's initial move from the seduction theory to the Oedipal theory. This development is offered as a prototype for the debates between conceptualisations of childhood traumatisation as a social problem of actual abuse occurring within dermed power relations, and theories which locate pathology purely within internal conflicts occurring inside the individual. Several criticisms of this shift are offered, and it s impact on later theory is considered. Here a contrast is offered between the theoretical approaches of Melanie Klein and Donald Winnicott, arguing for the usefulness of Winnicott's emphasis on environmental factors within psychodynamic theory. The impact of these theoretical approaches is illustrated through a critical evaluation of Freud's case study of Judge Schreber. Additional historical material is brought in to show the importance of environmental considerations ignored by Freud, and contrasting psychodynamic readings of the case are offered. As an alternative to purely intrapsychic approaches, a reinterpretation of certain strands of Critical Theory is then presented. Adorno's theory of the Authoritarian Personality and Marcuse' s concept of One-Dimensional Man are extended using Winnicott's formulations concerning psychological development. This leads to an examination of the question of the relationship between social structure and individual consciousness. Post-structuralist accounts of language and the construction of identity are explored. These are then developed drawing on theories of ideology, language and consciousness, integrating these with Winnicott's developmental theory to offer an alternative psychodynamic understanding of the relationship between social process and psychopathology. An attempt is made to reformulate - the notions of consciousness and the unconscious in terms of the possibilities and difficulties of representation within available social symbolic codes. In conclusion the it is argued that psychology needs to integrate critical social theory and contemporary understandings of the social construction of consciousness in order to become a meaning force in positive social transformation.
- Full Text:
- Date Issued: 1995
Foreign labour migration to South Africa after apartheid: continuity or change?
- Authors: Myataza, Lutando Samuel
- Date: 1995
- Subjects: Foreign workers -- South Africa , Black people -- Employment -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2813 , http://hdl.handle.net/10962/d1003023 , Foreign workers -- South Africa , Black people -- Employment -- South Africa
- Description: Migrant labour constitutes one of the perennial problems of the political economy of the Southern African region. The movement of people between their home countries and the gold mines in South Africa is over a hundred years old. In this sense, labour migrancy predates apartheid, and is now threatening to outlive it. Migrants working in South Africa make up a sizable proportion of the total wage earning population of the neighbouring countries (Botswana, Lesotho, Swaziland, Mozambique and Malawi). Their entrenched dependence on migrant-based revenue has made them vulnerable to fluctuations in labour flows to the gold mines (Davies, 1992) The developments since the first quarter of 1988 have altered the terms of debate on migrant labour. With the independence of Namibia and the establishment of a new government in South Africa, to name but a few, the pattern of future regional cooperation has become a central issue. Likewise, the debate about labour migrancy has entered a new phase. Given the current political changes, and yet to take place, this study constitutes a modest attempt to contribute to the debate by examining the prospects of restructuring of labour migrancy to the gold mining industry. The central aim of this undertaking will be to establish what the future holds for the foreign component of the labour force. The focus here will be on the emerging debates in this area, policy pronouncements by major stakeholders, the Chamber of Mines and the new Government of National Unity. However, an informed forecast on the future of labour migrancy is possible only if we establish the conditions that created it in the first place; how it has been constructed overtime; and the successive moments of transformation in the system. In this regard, this study will locate the origins and development of labour migration in its proper historical context ~ before drawing conclusions about the future of the system. In this scholarly pursuit one does not pretend to break a new ground but rather to re-interpret the current literature on migration and establish whether the current pattern of labour migration will change. The research will be based mainly on secondary materials, drawing extensively on published materials, journals and articles.
- Full Text:
- Date Issued: 1995
- Authors: Myataza, Lutando Samuel
- Date: 1995
- Subjects: Foreign workers -- South Africa , Black people -- Employment -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2813 , http://hdl.handle.net/10962/d1003023 , Foreign workers -- South Africa , Black people -- Employment -- South Africa
- Description: Migrant labour constitutes one of the perennial problems of the political economy of the Southern African region. The movement of people between their home countries and the gold mines in South Africa is over a hundred years old. In this sense, labour migrancy predates apartheid, and is now threatening to outlive it. Migrants working in South Africa make up a sizable proportion of the total wage earning population of the neighbouring countries (Botswana, Lesotho, Swaziland, Mozambique and Malawi). Their entrenched dependence on migrant-based revenue has made them vulnerable to fluctuations in labour flows to the gold mines (Davies, 1992) The developments since the first quarter of 1988 have altered the terms of debate on migrant labour. With the independence of Namibia and the establishment of a new government in South Africa, to name but a few, the pattern of future regional cooperation has become a central issue. Likewise, the debate about labour migrancy has entered a new phase. Given the current political changes, and yet to take place, this study constitutes a modest attempt to contribute to the debate by examining the prospects of restructuring of labour migrancy to the gold mining industry. The central aim of this undertaking will be to establish what the future holds for the foreign component of the labour force. The focus here will be on the emerging debates in this area, policy pronouncements by major stakeholders, the Chamber of Mines and the new Government of National Unity. However, an informed forecast on the future of labour migrancy is possible only if we establish the conditions that created it in the first place; how it has been constructed overtime; and the successive moments of transformation in the system. In this regard, this study will locate the origins and development of labour migration in its proper historical context ~ before drawing conclusions about the future of the system. In this scholarly pursuit one does not pretend to break a new ground but rather to re-interpret the current literature on migration and establish whether the current pattern of labour migration will change. The research will be based mainly on secondary materials, drawing extensively on published materials, journals and articles.
- Full Text:
- Date Issued: 1995
Formulating the African National Congress' foreign investment policy in the transition to a post-apartheid South Africa: problems, pressures and constraints
- Authors: Carim, Xavier
- Date: 1995
- Subjects: Investments, Foreign -- South Africa , Political stability -- South Africa , African National Congress -- Foreign economic relations , South Africa -- Foreign economic relations , South Africa -- Economic policy , South Africa -- Economic conditions -- 1991-
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2764 , http://hdl.handle.net/10962/d1002974 , Investments, Foreign -- South Africa , Political stability -- South Africa , African National Congress -- Foreign economic relations , South Africa -- Foreign economic relations , South Africa -- Economic policy , South Africa -- Economic conditions -- 1991-
- Description: This study examines the wide-ranging and critical factors which have impacted on the African National Congress' (ANC) emerging foreign investment policy. It identifies and analyses the matrix of political and socio-economic factors which have combined at global and national levels to shape ANC policy perspectives towards foreign direct investment (FDI). In so doing, the study adopts an eclectic theoretical and methodological approach. It draws on various theoretical traditions to propose a framework that is heuristic and contingent, rather than axiomatic. With regard to foreign investment, in particular, it recommends a theoretical pluralism emphasising 'engagement' through praxis and sound political (state) action. The study argues that the ANC has reconsidered many of its basic assumptions on the nature of the post-apartheid economy and discusses the reasons for those shifts. The reasons include, in particular, global political and economic trends and the balance of forces in South Africa. These have combined to ensure the ANC's broad acceptance of an 'open-door policy' towards FDI so long as it occurs on terms not inconsistent with national objectives. The emerging policy sees the state playing an active role in encouraging and guiding FDI to specific areas and sectors supportive of broad-based development. Foreign investors will be encouraged to form joint ventures with emerging black businesses and agree to foster training, skills development and affirmative action. Harnessing the benefits of FDI will be important for the success of wider strategies designed to place the economy on a firmer, more sustainable growth path.
- Full Text:
- Date Issued: 1995
- Authors: Carim, Xavier
- Date: 1995
- Subjects: Investments, Foreign -- South Africa , Political stability -- South Africa , African National Congress -- Foreign economic relations , South Africa -- Foreign economic relations , South Africa -- Economic policy , South Africa -- Economic conditions -- 1991-
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2764 , http://hdl.handle.net/10962/d1002974 , Investments, Foreign -- South Africa , Political stability -- South Africa , African National Congress -- Foreign economic relations , South Africa -- Foreign economic relations , South Africa -- Economic policy , South Africa -- Economic conditions -- 1991-
- Description: This study examines the wide-ranging and critical factors which have impacted on the African National Congress' (ANC) emerging foreign investment policy. It identifies and analyses the matrix of political and socio-economic factors which have combined at global and national levels to shape ANC policy perspectives towards foreign direct investment (FDI). In so doing, the study adopts an eclectic theoretical and methodological approach. It draws on various theoretical traditions to propose a framework that is heuristic and contingent, rather than axiomatic. With regard to foreign investment, in particular, it recommends a theoretical pluralism emphasising 'engagement' through praxis and sound political (state) action. The study argues that the ANC has reconsidered many of its basic assumptions on the nature of the post-apartheid economy and discusses the reasons for those shifts. The reasons include, in particular, global political and economic trends and the balance of forces in South Africa. These have combined to ensure the ANC's broad acceptance of an 'open-door policy' towards FDI so long as it occurs on terms not inconsistent with national objectives. The emerging policy sees the state playing an active role in encouraging and guiding FDI to specific areas and sectors supportive of broad-based development. Foreign investors will be encouraged to form joint ventures with emerging black businesses and agree to foster training, skills development and affirmative action. Harnessing the benefits of FDI will be important for the success of wider strategies designed to place the economy on a firmer, more sustainable growth path.
- Full Text:
- Date Issued: 1995
Function of a cloned polyphenolase in organic synthesis
- Authors: Naidoo, Michael Joseph
- Date: 1995
- Subjects: Polyphenols , Catechol , Streptomacyes , Organic compounds -- Synthesis , Mutagenesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4042 , http://hdl.handle.net/10962/d1004103 , Polyphenols , Catechol , Streptomacyes , Organic compounds -- Synthesis , Mutagenesis
- Description: The enzyme polyphenolase, which catalyses the oxidation of phenols to catechols and subsequently dehydrogenates these to o-quinones, is widely distributed in nature. The multicopy plasmid vector pIJ702 contains a mel gene from Streptomyces antibioticus, that codes for the production of a polyphenol oxidase. The plasmid was isolated from Streptomyces lividans 66pIJ702 and subjected to a variety of mutagenic treatments in order to establish a structurefunction relationship for the polyphenolase enzymes. An attempt was made to engineer the polyphenolase enzyme by localized random mutagenesis in vitro of the mel gene on pIJ702, in order to alter properties like productivity, activity and substrate specificity. It was hoped to alter the amino acid sequence of the active site of the enzyme in order to facilitate catalysis in an organic environment. The plasmid was subsequently transformed into a plasmid-free Streptomyces strain, and enzyme production was carried out in batch culture systems, in order to determine the effect of the height treatment, and to isolate and propagate functional polyphenolase mutants for organic synthesis.
- Full Text:
- Date Issued: 1995
- Authors: Naidoo, Michael Joseph
- Date: 1995
- Subjects: Polyphenols , Catechol , Streptomacyes , Organic compounds -- Synthesis , Mutagenesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4042 , http://hdl.handle.net/10962/d1004103 , Polyphenols , Catechol , Streptomacyes , Organic compounds -- Synthesis , Mutagenesis
- Description: The enzyme polyphenolase, which catalyses the oxidation of phenols to catechols and subsequently dehydrogenates these to o-quinones, is widely distributed in nature. The multicopy plasmid vector pIJ702 contains a mel gene from Streptomyces antibioticus, that codes for the production of a polyphenol oxidase. The plasmid was isolated from Streptomyces lividans 66pIJ702 and subjected to a variety of mutagenic treatments in order to establish a structurefunction relationship for the polyphenolase enzymes. An attempt was made to engineer the polyphenolase enzyme by localized random mutagenesis in vitro of the mel gene on pIJ702, in order to alter properties like productivity, activity and substrate specificity. It was hoped to alter the amino acid sequence of the active site of the enzyme in order to facilitate catalysis in an organic environment. The plasmid was subsequently transformed into a plasmid-free Streptomyces strain, and enzyme production was carried out in batch culture systems, in order to determine the effect of the height treatment, and to isolate and propagate functional polyphenolase mutants for organic synthesis.
- Full Text:
- Date Issued: 1995
Fuzzy ideals in commutative rings
- Authors: Sekaran, Rajakrishnar
- Date: 1995
- Subjects: Commutative rings , Fuzzy algebra
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5407 , http://hdl.handle.net/10962/d1005221 , Commutative rings , Fuzzy algebra
- Description: In this thesis, we are concerned with various aspects of fuzzy ideals of commutative rings. The central theorem is that of primary decomposition of a fuzzy ideal as an intersection of fuzzy primary ideals in a commutative Noetherian ring. We establish the existence and the two uniqueness theorems of primary decomposition of any fuzzy ideal with membership value 1 at the zero element. In proving this central result, we build up the necessary tools such as fuzzy primary ideals and the related concept of fuzzy maximal ideals, fuzzy prime ideals and fuzzy radicals. Another approach explores various characterizations of fuzzy ideals, namely, generation and level cuts of fuzzy ideals, relation between fuzzy ideals, congruences and quotient fuzzy rings. We also tie up several authors' seemingly different definitions of fuzzy prime, primary, semiprimary and fuzzy radicals available in the literature and show some of their equivalences and implications, providing counter-examples where certain implications fail.
- Full Text:
- Date Issued: 1995
- Authors: Sekaran, Rajakrishnar
- Date: 1995
- Subjects: Commutative rings , Fuzzy algebra
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5407 , http://hdl.handle.net/10962/d1005221 , Commutative rings , Fuzzy algebra
- Description: In this thesis, we are concerned with various aspects of fuzzy ideals of commutative rings. The central theorem is that of primary decomposition of a fuzzy ideal as an intersection of fuzzy primary ideals in a commutative Noetherian ring. We establish the existence and the two uniqueness theorems of primary decomposition of any fuzzy ideal with membership value 1 at the zero element. In proving this central result, we build up the necessary tools such as fuzzy primary ideals and the related concept of fuzzy maximal ideals, fuzzy prime ideals and fuzzy radicals. Another approach explores various characterizations of fuzzy ideals, namely, generation and level cuts of fuzzy ideals, relation between fuzzy ideals, congruences and quotient fuzzy rings. We also tie up several authors' seemingly different definitions of fuzzy prime, primary, semiprimary and fuzzy radicals available in the literature and show some of their equivalences and implications, providing counter-examples where certain implications fail.
- Full Text:
- Date Issued: 1995
Geochemical exploration in calcrete terrains
- Authors: Krug, Mark Alan
- Date: 1995 , 2013-10-02
- Subjects: Duricrusts , Silcrete , Geochemical prospecting
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5026 , http://hdl.handle.net/10962/d1006891 , Duricrusts , Silcrete , Geochemical prospecting
- Description: This work takes a look at some of the literature on calcretes and especially the problem of geochemical exploration in calcrete terrains. The conclusion that will be reached is that exploration in calcrete terrains is not futile and that provided the explorationist is aware of the types of calcrete and their genetic implications calcrete can be used as a sampling medium and anomalies can be detected through calcrete (p.1.) , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1995
- Authors: Krug, Mark Alan
- Date: 1995 , 2013-10-02
- Subjects: Duricrusts , Silcrete , Geochemical prospecting
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5026 , http://hdl.handle.net/10962/d1006891 , Duricrusts , Silcrete , Geochemical prospecting
- Description: This work takes a look at some of the literature on calcretes and especially the problem of geochemical exploration in calcrete terrains. The conclusion that will be reached is that exploration in calcrete terrains is not futile and that provided the explorationist is aware of the types of calcrete and their genetic implications calcrete can be used as a sampling medium and anomalies can be detected through calcrete (p.1.) , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1995
Geological characteristics of selected disseminated sediment-hosted gold deposits in Nevada, U.S.A. : in search of an exploration model
- Authors: Skead, Michael Bethel
- Date: 1995 , 2013-10-07
- Subjects: Gold ores -- Geology -- Nevada
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5036 , http://hdl.handle.net/10962/d1007414 , Gold ores -- Geology -- Nevada
- Description: Sediment-hosted disseminated gold deposits in Nevada, western United States are major gold sources and contain reserves in excess of 1 500 metric tons of gold (Percival et aI., 1988). Discovery of these deposit types continues at a pace, with Placer Dome announcing a mojor discovery, Pipeline, to the south of the Gold Acres Mine, along the Battle Mountain - Eureka Trend in 1994 (The Northern Miner, 1994). Host sediments favoured for disseminated gold mineralisation are thinly bedded silty limestones , carbonate debris flows and to a lesser extent shale, chert and sandstone. The distribution of mineralisation is controlled essentially by the intersection of high-angle faults, which acted as conduits for hydrothermal fluids, with favourable host lithologies, anticlines, low-angle faults and other high-angle faults. Geochemical signature for these deposits is simple being Au, Ag, As, Sb, Hg, Tl, Te, F and Ba, but individual element concentrations vary greatly between and within deposits. Age of mineralisation is cause for considerable debate, and ages ranging between isotopic dates of approximately 117 Ma to early to mid-Tertiary (30-40 Ma) are proposed. Most of these deposits are situated along three major trends namely the Carlin, Battle Mountain - Eureka and Getchell trends. The Battle Mountain - Eureka trend and, to a lesser extent the Carlin trend, are defined by major linear aeromagnetic and gravity anomalies , which are believed to reflect deep-seated structures. Most deposits are hosted in autochthonous Devonian, thinly bedded, silty limestones that occur as windows through what is believed to be allochthonous Ordovician siliciclastic sediments, which were transported from west to east along the Roberts Mountains thrust during the late-Devonian Antler Orogeny. However, recent fossil dating of what were thought to be Ordivician siliciclastic sediments, gives a Devonian age. This questions the age of Ordivician sediments at the other deposits and the interpretation of the structural windows in which deposits are located. Fault-bounded, proximal, carbonate debris-flow breccias are now recognised as a major host to mineralisation. These debris flow breccias, together with interbedded carbonate and siliciclastic sediments, carbonaceous sediments and soft sediment deformation are all characteristics of lithologies in pull-apart basins which develop along a major strike slip faults. It is proposed that sediment-hosted disseminated gold mineralisation is controlled by the distribution of deep-seated long-lived, predominantly right-lateral strike-slip faults. It is along these strike-slip faults that syn-sedimentary pull-apart basins developed, within which sediments favoured by epigenetic gold mineralisation were deposited. These pull-apart basins were then overprinted by post-depositional extensional structures, such as negative flower structures. Igneous intrusions and hydrothermal cells have exploited these extensional structures in both compressional and extensional regional tectonic regimes. This model explains the characteristics of the host sediment at many of the deposits, the spatial relationship between igneous intrusion and mineralisation, spanning the period Cretaceous through to mid-Tertiary, the distribution of deposits as districts along major regional trends and why hydrothermal activity is noted between deposit districts but with no complementary mineralisation. Mineralisation is controlled predominantly by high angle structures and although the recent age for mineralisation at the Betze/Post deposit is ~ 117 Ma (Arehart et aI., 1993a), placing it in the compressional Sevier Orogeny, these high-angle structures would be developed within local extensional tectonic domains as described above. This model can, and should, be applied to other areas of the world where similar geological features exist. In exploring for these deposits in Nevada the distribution of Ordovician siliciclastic sediments should be reviewed, especially where spatially associated with deep regional structures. Ordovician sediments have historically been regarded as unfavourable, hence large areas for potential exploration have been ignored but with new ages for these sediments this opens large areas for potential discoveries. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1995
- Authors: Skead, Michael Bethel
- Date: 1995 , 2013-10-07
- Subjects: Gold ores -- Geology -- Nevada
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5036 , http://hdl.handle.net/10962/d1007414 , Gold ores -- Geology -- Nevada
- Description: Sediment-hosted disseminated gold deposits in Nevada, western United States are major gold sources and contain reserves in excess of 1 500 metric tons of gold (Percival et aI., 1988). Discovery of these deposit types continues at a pace, with Placer Dome announcing a mojor discovery, Pipeline, to the south of the Gold Acres Mine, along the Battle Mountain - Eureka Trend in 1994 (The Northern Miner, 1994). Host sediments favoured for disseminated gold mineralisation are thinly bedded silty limestones , carbonate debris flows and to a lesser extent shale, chert and sandstone. The distribution of mineralisation is controlled essentially by the intersection of high-angle faults, which acted as conduits for hydrothermal fluids, with favourable host lithologies, anticlines, low-angle faults and other high-angle faults. Geochemical signature for these deposits is simple being Au, Ag, As, Sb, Hg, Tl, Te, F and Ba, but individual element concentrations vary greatly between and within deposits. Age of mineralisation is cause for considerable debate, and ages ranging between isotopic dates of approximately 117 Ma to early to mid-Tertiary (30-40 Ma) are proposed. Most of these deposits are situated along three major trends namely the Carlin, Battle Mountain - Eureka and Getchell trends. The Battle Mountain - Eureka trend and, to a lesser extent the Carlin trend, are defined by major linear aeromagnetic and gravity anomalies , which are believed to reflect deep-seated structures. Most deposits are hosted in autochthonous Devonian, thinly bedded, silty limestones that occur as windows through what is believed to be allochthonous Ordovician siliciclastic sediments, which were transported from west to east along the Roberts Mountains thrust during the late-Devonian Antler Orogeny. However, recent fossil dating of what were thought to be Ordivician siliciclastic sediments, gives a Devonian age. This questions the age of Ordivician sediments at the other deposits and the interpretation of the structural windows in which deposits are located. Fault-bounded, proximal, carbonate debris-flow breccias are now recognised as a major host to mineralisation. These debris flow breccias, together with interbedded carbonate and siliciclastic sediments, carbonaceous sediments and soft sediment deformation are all characteristics of lithologies in pull-apart basins which develop along a major strike slip faults. It is proposed that sediment-hosted disseminated gold mineralisation is controlled by the distribution of deep-seated long-lived, predominantly right-lateral strike-slip faults. It is along these strike-slip faults that syn-sedimentary pull-apart basins developed, within which sediments favoured by epigenetic gold mineralisation were deposited. These pull-apart basins were then overprinted by post-depositional extensional structures, such as negative flower structures. Igneous intrusions and hydrothermal cells have exploited these extensional structures in both compressional and extensional regional tectonic regimes. This model explains the characteristics of the host sediment at many of the deposits, the spatial relationship between igneous intrusion and mineralisation, spanning the period Cretaceous through to mid-Tertiary, the distribution of deposits as districts along major regional trends and why hydrothermal activity is noted between deposit districts but with no complementary mineralisation. Mineralisation is controlled predominantly by high angle structures and although the recent age for mineralisation at the Betze/Post deposit is ~ 117 Ma (Arehart et aI., 1993a), placing it in the compressional Sevier Orogeny, these high-angle structures would be developed within local extensional tectonic domains as described above. This model can, and should, be applied to other areas of the world where similar geological features exist. In exploring for these deposits in Nevada the distribution of Ordovician siliciclastic sediments should be reviewed, especially where spatially associated with deep regional structures. Ordovician sediments have historically been regarded as unfavourable, hence large areas for potential exploration have been ignored but with new ages for these sediments this opens large areas for potential discoveries. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1995
Insect herbivores associated with Senecio pterophorus and Senecio inaequidens at Butterworth, South Africa
- Muwanga-Zake, Johnnie Wycliffe Frank
- Authors: Muwanga-Zake, Johnnie Wycliffe Frank
- Date: 1995
- Subjects: Senecio -- South Africa -- Eastern Cape -- Butterworth Insect-plant relationships -- South Africa -- Eastern Cape -- Butterworth Insects -- South Africa -- Eastern Cape -- Butterworth -- Host plants Insects -- South Africa -- Eastern Cape -- Butterworth -- Food Lixus -- biology Insect-plant relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5716 , http://hdl.handle.net/10962/d1005402
- Description: This study lists and compares insect herbivores of Senecio inaequidens and of Senecio pterophorus around Butterworth by 18 Tables and 38 Figures. The two plant species are widely distributed around Southern Africa. Samples were taken during a month of each of the four main seasons of the year, which in Butterworth did not show much difference in temperature. The two plant species shared generalist insect herbivores most of which were also on other surrounding plant species. The population of most of the herbivores was affected by the seasons while the two plant species were not seasonal, thus the correlation between insect herbivores and the two plant species was not significant. The heavier, and apparently chemically protected species, Senecio pterophorus, accommodated borers and supported a wider variety of but fewer individual insects. Senecio pterophorus could be having more defences but had more serious herbivores. There were no serious insect herbivores that could control the abundance of the two plant species around Butterworth. Anatomical differences between S. pterophorus and S. inaequidens could be highlighted by the kind and number of insect herbivores found upon them. Similarities were more difficult to identify through studying their insect herbivores. The biology of one serious weed, Lixus sp., on Senecio pterophorus was studied in more detail. The life cycle of the weevil was found to be synchronised with that of the host. This weevil shared the stems with other species of weevils with no indication of competition.
- Full Text:
- Date Issued: 1995
- Authors: Muwanga-Zake, Johnnie Wycliffe Frank
- Date: 1995
- Subjects: Senecio -- South Africa -- Eastern Cape -- Butterworth Insect-plant relationships -- South Africa -- Eastern Cape -- Butterworth Insects -- South Africa -- Eastern Cape -- Butterworth -- Host plants Insects -- South Africa -- Eastern Cape -- Butterworth -- Food Lixus -- biology Insect-plant relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5716 , http://hdl.handle.net/10962/d1005402
- Description: This study lists and compares insect herbivores of Senecio inaequidens and of Senecio pterophorus around Butterworth by 18 Tables and 38 Figures. The two plant species are widely distributed around Southern Africa. Samples were taken during a month of each of the four main seasons of the year, which in Butterworth did not show much difference in temperature. The two plant species shared generalist insect herbivores most of which were also on other surrounding plant species. The population of most of the herbivores was affected by the seasons while the two plant species were not seasonal, thus the correlation between insect herbivores and the two plant species was not significant. The heavier, and apparently chemically protected species, Senecio pterophorus, accommodated borers and supported a wider variety of but fewer individual insects. Senecio pterophorus could be having more defences but had more serious herbivores. There were no serious insect herbivores that could control the abundance of the two plant species around Butterworth. Anatomical differences between S. pterophorus and S. inaequidens could be highlighted by the kind and number of insect herbivores found upon them. Similarities were more difficult to identify through studying their insect herbivores. The biology of one serious weed, Lixus sp., on Senecio pterophorus was studied in more detail. The life cycle of the weevil was found to be synchronised with that of the host. This weevil shared the stems with other species of weevils with no indication of competition.
- Full Text:
- Date Issued: 1995
Interacting with Shakespeare's figurative language: a project in materials development for the L2 classroom
- Authors: Lenahan, Patrick
- Date: 1995
- Subjects: Shakespeare, William, 1564-1616 -- Language Shakespeare, William, 1564-1616 -- Study and teaching English literature -- Study and teaching -- South Africa -- Foreign speakers English language -- Study and teaching -- South Africa -- Foreign speakers
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1581 , http://hdl.handle.net/10962/d1003463
- Description: This project arises from recent initiatives aimed at transforming Shakespeare studies in South African high schools, so as to make those studies more learner-centred and interactive, as well as a more useful communicative language-learning experience for second-language (L2) students. It is this interactive methodology that the present project seeks to extend to the relatively neglected area of Shakespeare's figurative language. Drawing on schema theory and response-based approaches to literature teaching, the project shows that figurative language is especially conducive to interactive treatment, whereby students might be encouraged to make sense of metaphors and similes out of their "background knowledge". Guidelines are indicated for putting this into practice in the L2 classroom; and on the basis of these guidelines, materials are developed for an interactive approach to Shakespeare's figurative language. The central phase in this development process involves trying out the materials in five African high schools and then analysing the data collected from them. The classroom try-outs were profitable in so far as they raised issues that had been overlooked in the earlier, theoretical, stage of the development process. A good overall response to the materials' learner-centred approach was indicated, although students experienced difficulties with certain essential tasks. Most seriously, while the materials were successful in accessing students' background knowledge in the form of associations, they were less successful in getting students to use this knowiedge in interpreting metaphors for themselves. Reasons for this feature, and others, are considered and solutions posited. Recommendations for implementing the materials in a larger teaching programme are made.
- Full Text:
- Date Issued: 1995
- Authors: Lenahan, Patrick
- Date: 1995
- Subjects: Shakespeare, William, 1564-1616 -- Language Shakespeare, William, 1564-1616 -- Study and teaching English literature -- Study and teaching -- South Africa -- Foreign speakers English language -- Study and teaching -- South Africa -- Foreign speakers
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1581 , http://hdl.handle.net/10962/d1003463
- Description: This project arises from recent initiatives aimed at transforming Shakespeare studies in South African high schools, so as to make those studies more learner-centred and interactive, as well as a more useful communicative language-learning experience for second-language (L2) students. It is this interactive methodology that the present project seeks to extend to the relatively neglected area of Shakespeare's figurative language. Drawing on schema theory and response-based approaches to literature teaching, the project shows that figurative language is especially conducive to interactive treatment, whereby students might be encouraged to make sense of metaphors and similes out of their "background knowledge". Guidelines are indicated for putting this into practice in the L2 classroom; and on the basis of these guidelines, materials are developed for an interactive approach to Shakespeare's figurative language. The central phase in this development process involves trying out the materials in five African high schools and then analysing the data collected from them. The classroom try-outs were profitable in so far as they raised issues that had been overlooked in the earlier, theoretical, stage of the development process. A good overall response to the materials' learner-centred approach was indicated, although students experienced difficulties with certain essential tasks. Most seriously, while the materials were successful in accessing students' background knowledge in the form of associations, they were less successful in getting students to use this knowiedge in interpreting metaphors for themselves. Reasons for this feature, and others, are considered and solutions posited. Recommendations for implementing the materials in a larger teaching programme are made.
- Full Text:
- Date Issued: 1995
Intercultural communication on a passenger aircraft flight deck: a qualitative study set in the context of South African corporate affirmative action policy
- Authors: Waite, Michele Jeannette
- Date: 1995
- Subjects: Intercultural communication South Africa , Language and culture South Africa , Communication and culture South Africa , Affirmative action programs South Africa , Air pilots South Africa Language , Flight crews South Africa Language ,
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3082 , http://hdl.handle.net/10962/d1002591
- Description: The recent emphasis on Affirmative Action policies in South African corporations has illuminated the issue of cultural diversity in organizations and the difficulties arising from this transition and change. One such difficulty is the problem of intercultural communication where miscommunication can have life-threatening consequences. This problem is particularly critical in the operational environment of the passenger aircraft flight deck where small group factors, as well as intercultural issues must be taken into account. In this thesis the problem of intercultural communication in such contexts as a consequence of Affirmative Action is examined. The data for this thesis comprises the communication of five culturally diverse flight crews. A multi-method was used for the gathering and analysis of this data. This involved the use of video-taped flight simulator sessions, and personal interviews with each crew member. The subjects themselves were involved in identifying key communication problems from the video and interpretation of the data. Three levels of analysis were used in the interpretation of the findings - the individual, the group and the organization. The factors which affected communication at each level in the small, culturally diverse group are highlighted. Furthermore, factors which influence the climate in which communication takes place are also considered. The implications for Affirmative Action are discussed.
- Full Text:
- Date Issued: 1995
- Authors: Waite, Michele Jeannette
- Date: 1995
- Subjects: Intercultural communication South Africa , Language and culture South Africa , Communication and culture South Africa , Affirmative action programs South Africa , Air pilots South Africa Language , Flight crews South Africa Language ,
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3082 , http://hdl.handle.net/10962/d1002591
- Description: The recent emphasis on Affirmative Action policies in South African corporations has illuminated the issue of cultural diversity in organizations and the difficulties arising from this transition and change. One such difficulty is the problem of intercultural communication where miscommunication can have life-threatening consequences. This problem is particularly critical in the operational environment of the passenger aircraft flight deck where small group factors, as well as intercultural issues must be taken into account. In this thesis the problem of intercultural communication in such contexts as a consequence of Affirmative Action is examined. The data for this thesis comprises the communication of five culturally diverse flight crews. A multi-method was used for the gathering and analysis of this data. This involved the use of video-taped flight simulator sessions, and personal interviews with each crew member. The subjects themselves were involved in identifying key communication problems from the video and interpretation of the data. Three levels of analysis were used in the interpretation of the findings - the individual, the group and the organization. The factors which affected communication at each level in the small, culturally diverse group are highlighted. Furthermore, factors which influence the climate in which communication takes place are also considered. The implications for Affirmative Action are discussed.
- Full Text:
- Date Issued: 1995
Interspecific interactions of the sea urchin Parechinus angulosus and the effect of variations in microhabitat availability
- Authors: Farquhar, Michael Robert
- Date: 1995
- Subjects: Sea urchins , Niche (Ecology) , Sea urchins -- Habitat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5720 , http://hdl.handle.net/10962/d1005406 , Sea urchins , Niche (Ecology) , Sea urchins -- Habitat
- Description: Intertidal populations of the sea urchin Parechinus angulosus were examined at seven sites along the south and east coasts of South Africa. At the five southerly sites, P. angulosus occurred in allopatry, while, at the two northerly sites, it occurred in sympatry with several species of Indopacific sea urchins. At the five southerly sites, there was a significant correlation of number of sea urchins per pool with the surface area of the overhang in that pool. This relationship broke down for P. angulosus at the two northerly sites, where there was a significant correlation between these two factors for Stomopneustes variolaris. The density of populations of P. angulosus at the two northerly sites, although not significantly different from all five southerly sites, were considerably lower. There was a significant difference between the mean size of populations at the seven sites. Although no direct evidence is available, a competitive interaction between these two species is proposed to explain the observed patterns of microhabitat utilization and densities. Two series of manipulative experiments were conducted at Kowie Point, where P. angulosus occurs in sympatry, The first, involved urchin removals from three experimental sites. The percentage cover of four functional algal groups was monitored in three experimental and three unaltered control sites over a three month period in spring (Sept. - Dec.) 1993. Two 100 point 0,25m² random point quadrats were thrown at each of the six sites at approximately monthly intervals over the experimental period. Since initial cover of algae varied at the six sites, ANOVA's of the change in percentage cover of four functional algal groups were used to determine treatment effects. No treatment effects were identified for any of the four functional algal groups. There was a strong negative correlation between the percentage cover of foliose algae and encrusting coralline algae, suggesting the possibility of a competitive relationship between them. The second, involved the experimental manipulation of microhabitat availability. The blocking of overhangs, by means of cement filled bags, resulted in an approximate 50% decrease in the total number of urchins in the two experimental pools, and the two control pools without overhangs. However, in the control pool with overhangs there was a slight increase in the number of urchins over the same period. Clearly, the presence of suitable shelters, is a prerequisite for the maintenance of dense intertidal population of P. angulosus. It is proposed that, due to the exposed nature of the South African coast, intertidal populations of P.angulosus are restricted to inhabiting suitable shelters from which they emerge to feed on passing drift algae. The implications of these findings are discussed in terms of current ecological literature.
- Full Text:
- Date Issued: 1995
- Authors: Farquhar, Michael Robert
- Date: 1995
- Subjects: Sea urchins , Niche (Ecology) , Sea urchins -- Habitat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5720 , http://hdl.handle.net/10962/d1005406 , Sea urchins , Niche (Ecology) , Sea urchins -- Habitat
- Description: Intertidal populations of the sea urchin Parechinus angulosus were examined at seven sites along the south and east coasts of South Africa. At the five southerly sites, P. angulosus occurred in allopatry, while, at the two northerly sites, it occurred in sympatry with several species of Indopacific sea urchins. At the five southerly sites, there was a significant correlation of number of sea urchins per pool with the surface area of the overhang in that pool. This relationship broke down for P. angulosus at the two northerly sites, where there was a significant correlation between these two factors for Stomopneustes variolaris. The density of populations of P. angulosus at the two northerly sites, although not significantly different from all five southerly sites, were considerably lower. There was a significant difference between the mean size of populations at the seven sites. Although no direct evidence is available, a competitive interaction between these two species is proposed to explain the observed patterns of microhabitat utilization and densities. Two series of manipulative experiments were conducted at Kowie Point, where P. angulosus occurs in sympatry, The first, involved urchin removals from three experimental sites. The percentage cover of four functional algal groups was monitored in three experimental and three unaltered control sites over a three month period in spring (Sept. - Dec.) 1993. Two 100 point 0,25m² random point quadrats were thrown at each of the six sites at approximately monthly intervals over the experimental period. Since initial cover of algae varied at the six sites, ANOVA's of the change in percentage cover of four functional algal groups were used to determine treatment effects. No treatment effects were identified for any of the four functional algal groups. There was a strong negative correlation between the percentage cover of foliose algae and encrusting coralline algae, suggesting the possibility of a competitive relationship between them. The second, involved the experimental manipulation of microhabitat availability. The blocking of overhangs, by means of cement filled bags, resulted in an approximate 50% decrease in the total number of urchins in the two experimental pools, and the two control pools without overhangs. However, in the control pool with overhangs there was a slight increase in the number of urchins over the same period. Clearly, the presence of suitable shelters, is a prerequisite for the maintenance of dense intertidal population of P. angulosus. It is proposed that, due to the exposed nature of the South African coast, intertidal populations of P.angulosus are restricted to inhabiting suitable shelters from which they emerge to feed on passing drift algae. The implications of these findings are discussed in terms of current ecological literature.
- Full Text:
- Date Issued: 1995
Parental preferences regarding medium of instruction in primary schools in the Nongoma district of Kwazulu-Natal
- Authors: Mhlanga, Samkelisiwe Isabel
- Date: 1995
- Subjects: Native language and education -- South Africa Language and education -- South Africa Education -- Parent participation -- South Africa Language policy -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1686 , http://hdl.handle.net/10962/d1003569
- Description: This thesis looks at what choice of medium of instruction (MOl) parents in a rural village in KwaZulu would make if they had the opportunity to choose. The background to this choice goes back to 1979, when Education and Training Act No. 90 established the mother tongue as MOl from Sub A to Std 2 in Department of Education and Training (DET) primary schools, followed by a sudden transition to English medium of instruction - (EMl) in Std 3. Though by 1990 98% of the schools had opted for EMI, conditions were not favourable for a sudden transition and the policy led to high drop-out rates. The problems encountered by teachers and learners were researched and documented by Macdonald in the Threshold Project Reports (1990). Although the Minister initially ignored the Project's findings, in May 1991 he admitted that his department's language policy was leading to serious educational disadvantages. The explosive situation that culminated in the 1976 school uprisings led to the amendment of the Act. There was concern among people involved in educational language policy that parents had not been given sufficient information to make informed educational choices. They feared that many parents would, largely out of ignorance, opt for straight-for-English, when in fact the conditions in the schools were not conducive to the success of that choice option. The widespread assumption about the parents choosing straight-for-English was based on anecdotal evidence. I decided to investigate this matter in the Nongoma area. My findings pointed to very healthy attitudes towards the mother tongue and there was even a measure of understanding of the place of mother tongue instruction in the beginner classes. But even though the respondents wanted their language to be respected, they also wanted their children to acquire a good education in English, so as to be eligible for jobs in an economy that emphasises the importance of English.
- Full Text:
- Date Issued: 1995
- Authors: Mhlanga, Samkelisiwe Isabel
- Date: 1995
- Subjects: Native language and education -- South Africa Language and education -- South Africa Education -- Parent participation -- South Africa Language policy -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1686 , http://hdl.handle.net/10962/d1003569
- Description: This thesis looks at what choice of medium of instruction (MOl) parents in a rural village in KwaZulu would make if they had the opportunity to choose. The background to this choice goes back to 1979, when Education and Training Act No. 90 established the mother tongue as MOl from Sub A to Std 2 in Department of Education and Training (DET) primary schools, followed by a sudden transition to English medium of instruction - (EMl) in Std 3. Though by 1990 98% of the schools had opted for EMI, conditions were not favourable for a sudden transition and the policy led to high drop-out rates. The problems encountered by teachers and learners were researched and documented by Macdonald in the Threshold Project Reports (1990). Although the Minister initially ignored the Project's findings, in May 1991 he admitted that his department's language policy was leading to serious educational disadvantages. The explosive situation that culminated in the 1976 school uprisings led to the amendment of the Act. There was concern among people involved in educational language policy that parents had not been given sufficient information to make informed educational choices. They feared that many parents would, largely out of ignorance, opt for straight-for-English, when in fact the conditions in the schools were not conducive to the success of that choice option. The widespread assumption about the parents choosing straight-for-English was based on anecdotal evidence. I decided to investigate this matter in the Nongoma area. My findings pointed to very healthy attitudes towards the mother tongue and there was even a measure of understanding of the place of mother tongue instruction in the beginner classes. But even though the respondents wanted their language to be respected, they also wanted their children to acquire a good education in English, so as to be eligible for jobs in an economy that emphasises the importance of English.
- Full Text:
- Date Issued: 1995
Petrogenesis of the New Amalfi sheet a highly differentiated Karoo intrusion
- Authors: Williams, Craig Milton
- Date: 1995
- Subjects: Petrogenesis -- South Africa -- Eastern Cape -- Karoo , Geochemistry -- South Africa -- Eastern Cape -- Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4985 , http://hdl.handle.net/10962/d1005597 , Petrogenesis -- South Africa -- Eastern Cape -- Karoo , Geochemistry -- South Africa -- Eastern Cape -- Karoo
- Description: The New Amalfi Sheet is a highly differentiated tholeiitic intrusion which is situated between the towns of Matatiele and Swartberg in East Griqualand. It lies within the Central area of the Karoo Igneous Province. Rock types range from dolerites at the base and top through to a highly differentiated granophyre which is found as a 'sandwich horizon' within the top half of the sheet. The most evolved granophyre represents 15.86% of the initial liquid, which was found to be very similar in composition to the average Lesotho-type magma of Marsh and Eales (1984). The paragenetic sequence was found to be chromite → olivine → plagioclase → pigeonite and augite. Cumulus magnetite and ilmenite enter the paragenetic sequence together with immiscible sulphide droplets after 35% crystallization. In the late stages of crystallization, augite changes composition towards ferrohedenbergite. The reappearance of iron-rich olivine coincides with the disappearance of pigeonite and apatite appears as a cumulus phase for the first time after 70% crystallization. Granophyric intergrowth, which contains coarse perthitic Kfeldspar, becomes the most abundant modal entity within the most evolved granophyres. Differentiation was dominated by fractionation of plagioclase and pyroxene, with subordinate olivine and opaque-oxide fractionation. A minor amount of assimilation of country rock occurred within the topmost granophyres. The intrusion has been dated, using the Rb-Sr isochron method, at 178.37 ± 5.52 Ma. Extensive subsolidus deuteric alteration has resulted in the formation of a complete series of hydrothermally altered clinopyroxenes which are enriched in CaO but depleted in TiO₂ compared to the unaltered magmatic clinopyroxenes. It has also resulted in the formation of abundant vermiform ilmenite in the most evolved rocks, recognized by the fact that this phase is enriched in MnO compared to magmatic ilmenites. The very iron-rich orthopyroxene, ferrohypersthene, was found to have crystallized, during cooling of the sheet from the intercumulus liquid. Olivine in the dolerite re-equilibrated with the intercumulus liquid, becoming more iron-rich in composition.
- Full Text:
- Date Issued: 1995
- Authors: Williams, Craig Milton
- Date: 1995
- Subjects: Petrogenesis -- South Africa -- Eastern Cape -- Karoo , Geochemistry -- South Africa -- Eastern Cape -- Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4985 , http://hdl.handle.net/10962/d1005597 , Petrogenesis -- South Africa -- Eastern Cape -- Karoo , Geochemistry -- South Africa -- Eastern Cape -- Karoo
- Description: The New Amalfi Sheet is a highly differentiated tholeiitic intrusion which is situated between the towns of Matatiele and Swartberg in East Griqualand. It lies within the Central area of the Karoo Igneous Province. Rock types range from dolerites at the base and top through to a highly differentiated granophyre which is found as a 'sandwich horizon' within the top half of the sheet. The most evolved granophyre represents 15.86% of the initial liquid, which was found to be very similar in composition to the average Lesotho-type magma of Marsh and Eales (1984). The paragenetic sequence was found to be chromite → olivine → plagioclase → pigeonite and augite. Cumulus magnetite and ilmenite enter the paragenetic sequence together with immiscible sulphide droplets after 35% crystallization. In the late stages of crystallization, augite changes composition towards ferrohedenbergite. The reappearance of iron-rich olivine coincides with the disappearance of pigeonite and apatite appears as a cumulus phase for the first time after 70% crystallization. Granophyric intergrowth, which contains coarse perthitic Kfeldspar, becomes the most abundant modal entity within the most evolved granophyres. Differentiation was dominated by fractionation of plagioclase and pyroxene, with subordinate olivine and opaque-oxide fractionation. A minor amount of assimilation of country rock occurred within the topmost granophyres. The intrusion has been dated, using the Rb-Sr isochron method, at 178.37 ± 5.52 Ma. Extensive subsolidus deuteric alteration has resulted in the formation of a complete series of hydrothermally altered clinopyroxenes which are enriched in CaO but depleted in TiO₂ compared to the unaltered magmatic clinopyroxenes. It has also resulted in the formation of abundant vermiform ilmenite in the most evolved rocks, recognized by the fact that this phase is enriched in MnO compared to magmatic ilmenites. The very iron-rich orthopyroxene, ferrohypersthene, was found to have crystallized, during cooling of the sheet from the intercumulus liquid. Olivine in the dolerite re-equilibrated with the intercumulus liquid, becoming more iron-rich in composition.
- Full Text:
- Date Issued: 1995
Physiological, perceptual and performance responses to competitive stress in individual and team sports among youth sport participants
- Authors: Swart, Kamilla
- Date: 1995
- Subjects: Sports -- Psychological aspects , Competition (Psychology) in adolescence -- Testing , Stress in adolescence -- Testing , Anxiety in adolescence -- Testing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5169 , http://hdl.handle.net/10962/d1016257
- Description: Due to limited research in the field testing of youth sport, specifically with regard to females, this project was delimited to the examination of the responses of youth participants in relation to the nature of the sport, when participating under conditions of competitive stress. It further attempted to elucidate gender related differences within these responses. Forty four male and female subjects aged 15 - 19 years volunteered to participate in this study; all pupils were First team members who competed in a variety of team and individual sports. Subjects participated in a battery of psychological, physiological and performance tests before and after competing in a practice session and during competitive league matches. The data were statistically analyzed for the various parameters under investigation. The results of the Sport Competition Anxiety Test indicated that the females, and the male team players had the highest competitive anxiety traits (CTA). The Task and Ego Orientation in Sport Questionnaire scores showed that most subjects have a general dominance of task orientation to sport. The results of the Physical Self-Perception Profile revealed that the females' poorer perception of their physical was not as substantial as their difference in CTA compared to males. Team participants, particularly the males, had a poorer self-image than individual sport counterparts. Elevated heart rate responses were observed prior to performing in a competitive ambience, with significant increases in team, rather than individual, players. The Evaluation of Task Demands revealed that team players, especially the females, perceived the match demands as significantly higher than the individual athletes. No significant differences were observed in the players' Evaluation of Perceived Strain. Neither were any significant differences observed in the players' ratings of their performance. In conclusion, although differences appear to exist between male and female responses within a competitive ambience, the magnitude of difference was far more notable between team and individual responses to competitive stress, particularly for competitive matches as opposed to practice sessions.
- Full Text:
- Date Issued: 1995
- Authors: Swart, Kamilla
- Date: 1995
- Subjects: Sports -- Psychological aspects , Competition (Psychology) in adolescence -- Testing , Stress in adolescence -- Testing , Anxiety in adolescence -- Testing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5169 , http://hdl.handle.net/10962/d1016257
- Description: Due to limited research in the field testing of youth sport, specifically with regard to females, this project was delimited to the examination of the responses of youth participants in relation to the nature of the sport, when participating under conditions of competitive stress. It further attempted to elucidate gender related differences within these responses. Forty four male and female subjects aged 15 - 19 years volunteered to participate in this study; all pupils were First team members who competed in a variety of team and individual sports. Subjects participated in a battery of psychological, physiological and performance tests before and after competing in a practice session and during competitive league matches. The data were statistically analyzed for the various parameters under investigation. The results of the Sport Competition Anxiety Test indicated that the females, and the male team players had the highest competitive anxiety traits (CTA). The Task and Ego Orientation in Sport Questionnaire scores showed that most subjects have a general dominance of task orientation to sport. The results of the Physical Self-Perception Profile revealed that the females' poorer perception of their physical was not as substantial as their difference in CTA compared to males. Team participants, particularly the males, had a poorer self-image than individual sport counterparts. Elevated heart rate responses were observed prior to performing in a competitive ambience, with significant increases in team, rather than individual, players. The Evaluation of Task Demands revealed that team players, especially the females, perceived the match demands as significantly higher than the individual athletes. No significant differences were observed in the players' Evaluation of Perceived Strain. Neither were any significant differences observed in the players' ratings of their performance. In conclusion, although differences appear to exist between male and female responses within a competitive ambience, the magnitude of difference was far more notable between team and individual responses to competitive stress, particularly for competitive matches as opposed to practice sessions.
- Full Text:
- Date Issued: 1995
Progestin receptor heterogeneity in a breast cancer cell line
- Authors: Levy, Anita Rochelle
- Date: 1995
- Subjects: Breast -- Cancer , Hormone receptors , Cancer cells -- Growth -- Regulation , Progesterone -- Receptors , Cellular control mechanisms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4039 , http://hdl.handle.net/10962/d1004100 , Breast -- Cancer , Hormone receptors , Cancer cells -- Growth -- Regulation , Progesterone -- Receptors , Cellular control mechanisms
- Description: Anti-oestrogens act via the oestrogen receptor whether they compete with the hormone for binding to the receptor and therefore interfere with DNA binding or inhibit transcriptional activity. These receptors exist as a large 85 complex and/or a small 45 form on sucrose density gradients. High performance ion-exchange chromatography has confirmed that the oestrogen and progestin complex is present in various isoforms. Progestin receptor heterogeneity could be influenced by the presence of oestrogens and anti-oestrogens in the culture media of hormone-dependent neoplastic cells. Cell culture methods offer the opportunity to test effects of specified components in repeated experiments on a homogeneous population of cells. MCF-7 and T47-D human breast cancer cell lines were conditioned to grow in a serum-free environment. There was no difference in cell proliferation rates, nor in their oestrogen or progestin receptor levels when compared to the same cells grown in conventional media. Receptors were present mainly in the large molecular 85 form. Both the MCF-7 and T47-D breast cancer cells showed an increase in proliferation rate with the addition of oestrogen or diethylstilbestrol. There was a corresponding loss of progestin receptor levels and an alteration in the high performance ion-exchange isoforms. Flow cytometry confirmed differences in the S-phase components of the cells following exposure to oestrogens. The proliferation rates of the cell lines as well as their progestin receptor levels decreased when treated with tamoxifen or the hydroxylated tamoxifen. There were marked changes on high performance ion-exchange chromatography profiles. DNA ploidy and S-phase showed signs of toxicity and there was an increase in cellular debris. The MCF-7 and T47-D human breast cancer cell line retained response to antioestrogen saturation.
- Full Text:
- Date Issued: 1995
- Authors: Levy, Anita Rochelle
- Date: 1995
- Subjects: Breast -- Cancer , Hormone receptors , Cancer cells -- Growth -- Regulation , Progesterone -- Receptors , Cellular control mechanisms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4039 , http://hdl.handle.net/10962/d1004100 , Breast -- Cancer , Hormone receptors , Cancer cells -- Growth -- Regulation , Progesterone -- Receptors , Cellular control mechanisms
- Description: Anti-oestrogens act via the oestrogen receptor whether they compete with the hormone for binding to the receptor and therefore interfere with DNA binding or inhibit transcriptional activity. These receptors exist as a large 85 complex and/or a small 45 form on sucrose density gradients. High performance ion-exchange chromatography has confirmed that the oestrogen and progestin complex is present in various isoforms. Progestin receptor heterogeneity could be influenced by the presence of oestrogens and anti-oestrogens in the culture media of hormone-dependent neoplastic cells. Cell culture methods offer the opportunity to test effects of specified components in repeated experiments on a homogeneous population of cells. MCF-7 and T47-D human breast cancer cell lines were conditioned to grow in a serum-free environment. There was no difference in cell proliferation rates, nor in their oestrogen or progestin receptor levels when compared to the same cells grown in conventional media. Receptors were present mainly in the large molecular 85 form. Both the MCF-7 and T47-D breast cancer cells showed an increase in proliferation rate with the addition of oestrogen or diethylstilbestrol. There was a corresponding loss of progestin receptor levels and an alteration in the high performance ion-exchange isoforms. Flow cytometry confirmed differences in the S-phase components of the cells following exposure to oestrogens. The proliferation rates of the cell lines as well as their progestin receptor levels decreased when treated with tamoxifen or the hydroxylated tamoxifen. There were marked changes on high performance ion-exchange chromatography profiles. DNA ploidy and S-phase showed signs of toxicity and there was an increase in cellular debris. The MCF-7 and T47-D human breast cancer cell line retained response to antioestrogen saturation.
- Full Text:
- Date Issued: 1995