'n Ondersoek na die funksie van die verteller ten opsigte van die aktualiteit en romanwêreld in sommige Afrikaanse romans
- Authors: Goosen, Ella Johanna
- Date: 1983
- Subjects: Afrikaans fiction -- History and criticism , First person narrative , Point of view (Literature)
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3620 , http://hdl.handle.net/10962/d1007176
- Description: Een van die fundamenteelste en belangrikste aspekte van 'n roman is die verteller. Die verhouding waarin die verteller tot die verhaalstof staan, die verteller se perspektief op die gebeure, die soort verteller en die manier waarop die verteller sy implisiete leser deur die organisasie van die verhaal definieer en betrek is almal bepalende faktore vir die struktuur, die styl en die ontwikkelingsgang van die roman. Joseph T. Shipley (1966:144) stel die saak so: "In die analysis of a speech or literary composition, nothing is more important than to determine precisely the voice or voices presented as speaking and the precise nature of the address (i.e. specific direction to a hearer, an addressee); for in every speech reference to a voice or voices and implication of address (i.e. reference to a process of speech, actual or imagined) is a part of the meaning, for the interpretation of which it supplies an indispensable control ".
- Full Text:
- Date Issued: 1983
- Authors: Goosen, Ella Johanna
- Date: 1983
- Subjects: Afrikaans fiction -- History and criticism , First person narrative , Point of view (Literature)
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3620 , http://hdl.handle.net/10962/d1007176
- Description: Een van die fundamenteelste en belangrikste aspekte van 'n roman is die verteller. Die verhouding waarin die verteller tot die verhaalstof staan, die verteller se perspektief op die gebeure, die soort verteller en die manier waarop die verteller sy implisiete leser deur die organisasie van die verhaal definieer en betrek is almal bepalende faktore vir die struktuur, die styl en die ontwikkelingsgang van die roman. Joseph T. Shipley (1966:144) stel die saak so: "In die analysis of a speech or literary composition, nothing is more important than to determine precisely the voice or voices presented as speaking and the precise nature of the address (i.e. specific direction to a hearer, an addressee); for in every speech reference to a voice or voices and implication of address (i.e. reference to a process of speech, actual or imagined) is a part of the meaning, for the interpretation of which it supplies an indispensable control ".
- Full Text:
- Date Issued: 1983
A phenomenological explication of the experience of counselling chronic callers in Life Line
- Authors: Matthis, Kenneth Skeen
- Date: 1983
- Subjects: Life Line Movement , Hotlines (Counseling)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3135 , http://hdl.handle.net/10962/d1006893 , Life Line Movement , Hotlines (Counseling)
- Full Text:
- Date Issued: 1983
- Authors: Matthis, Kenneth Skeen
- Date: 1983
- Subjects: Life Line Movement , Hotlines (Counseling)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3135 , http://hdl.handle.net/10962/d1006893 , Life Line Movement , Hotlines (Counseling)
- Full Text:
- Date Issued: 1983
A phenomenological investigation of the experience of "connected breathing"
- Authors: Van Wyk, Edmund
- Date: 1983
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:21110 , http://hdl.handle.net/10962/6439
- Description: My interest in "Connected Breathing" began with my own experience of this technique during a Gestalt workshop. It had a profound impact on me. I knew practically nothing about the theory, or lack of a theory, but my interest had been aroused. In my reading on the subject later, I was struck by the incongruence between my experience and what I read I was supposed to be experiencing. This led me "zu der Sache selbst." By using the phenomenological method, this study then is an attempt to come to an essential description of the experience of "Connected Breathing" and to make some evaluation of its usefulness as a psychotherapeutic technique. "Connected Breathing" is the main technique of the “Rebirthing”movement. It is, therefore, necessary to give some account of the development and theory, if any, of this movement. "Rebirthing" does not claim to be a psychotherapy. However, its technique of "Connected Breathing" does seem to have much in common with the techniques used by various established somatic psychotherapies. A brief overview of the development of some of these psychotherapies and their emphasis on breathing techniques is, therefore, also essential.
- Full Text:
- Date Issued: 1983
- Authors: Van Wyk, Edmund
- Date: 1983
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:21110 , http://hdl.handle.net/10962/6439
- Description: My interest in "Connected Breathing" began with my own experience of this technique during a Gestalt workshop. It had a profound impact on me. I knew practically nothing about the theory, or lack of a theory, but my interest had been aroused. In my reading on the subject later, I was struck by the incongruence between my experience and what I read I was supposed to be experiencing. This led me "zu der Sache selbst." By using the phenomenological method, this study then is an attempt to come to an essential description of the experience of "Connected Breathing" and to make some evaluation of its usefulness as a psychotherapeutic technique. "Connected Breathing" is the main technique of the “Rebirthing”movement. It is, therefore, necessary to give some account of the development and theory, if any, of this movement. "Rebirthing" does not claim to be a psychotherapy. However, its technique of "Connected Breathing" does seem to have much in common with the techniques used by various established somatic psychotherapies. A brief overview of the development of some of these psychotherapies and their emphasis on breathing techniques is, therefore, also essential.
- Full Text:
- Date Issued: 1983
A price analysis of vegetables on the East London municipal market
- Authors: Fraser, Gavin Cecil Gilbert
- Date: 1983
- Subjects: Agricultural prices -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1055 , http://hdl.handle.net/10962/d1006970 , Agricultural prices -- South Africa
- Description: [Introduction] Much of the information available on the prices of vegetables marketed through the fresh produce markets is of limited use to producers. This information contains the actual prices for individual years. This can obviously be taken as a guide to future prices but it does not necessarily mean that those prices are a true reflection of the general pattern. In this study an attempt will be made, firstly, to establish whether a general pattern exists in the prices of selected vegetables on the East London municipal market. This will be attempted by studying the prices obtained over the 1964-1979 period. Information of this nature can be used as a basis for the planning of future crops. Secondly, to determine the months which obtain the "best" prices for the selected vegetables taking into account the quantities supplied to the market.
- Full Text:
- Date Issued: 1983
- Authors: Fraser, Gavin Cecil Gilbert
- Date: 1983
- Subjects: Agricultural prices -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1055 , http://hdl.handle.net/10962/d1006970 , Agricultural prices -- South Africa
- Description: [Introduction] Much of the information available on the prices of vegetables marketed through the fresh produce markets is of limited use to producers. This information contains the actual prices for individual years. This can obviously be taken as a guide to future prices but it does not necessarily mean that those prices are a true reflection of the general pattern. In this study an attempt will be made, firstly, to establish whether a general pattern exists in the prices of selected vegetables on the East London municipal market. This will be attempted by studying the prices obtained over the 1964-1979 period. Information of this nature can be used as a basis for the planning of future crops. Secondly, to determine the months which obtain the "best" prices for the selected vegetables taking into account the quantities supplied to the market.
- Full Text:
- Date Issued: 1983
A review of asbestos resources
- Authors: Abbott, Paul
- Date: 1983
- Subjects: Asbestos Asbestos -- Geology Silicate minerals Mineralogy Chrysotile
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4910 , http://hdl.handle.net/10962/d1001570
- Full Text:
- Date Issued: 1983
- Authors: Abbott, Paul
- Date: 1983
- Subjects: Asbestos Asbestos -- Geology Silicate minerals Mineralogy Chrysotile
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4910 , http://hdl.handle.net/10962/d1001570
- Full Text:
- Date Issued: 1983
A study in local history: Grahamstown, 1883-1904
- Authors: Sellick, Rose-Mary
- Date: 1983
- Subjects: Grahamstown (South Africa) -- History
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2556 , http://hdl.handle.net/10962/d1002409 , Grahamstown (South Africa) -- History
- Description: [From the Preface]: A Study in Local History: Grahamstown 1883-1904 aims to draw into a coherent picture the threads of political attitudes, approaches to racial issues and changes confronting the late Victorians in Grahamstown, particularly in the areas of sanitation, public health and shifting commercial frontiers. The relation of local development to national affairs has been investigated, although attempts to define exactly how the former influenced the latter, and vice versa, would involve one in the proverbial chicken-and-egg syndrome. Let it suffice to say that an understanding of events in the microcosm, or locality, lends clarity to the cross-current of affairs at the national level. It begins at the point where Grahamstown's commercial importance in the Eastern Cape declines after a flourish of hopes in the prospects of the Port Alfred harbour as a means of bringing trade back to Grahamstown. The study concludes when the foundation of Rhodes University College in 1904, provides a new centre of development for the city.
- Full Text:
- Date Issued: 1983
- Authors: Sellick, Rose-Mary
- Date: 1983
- Subjects: Grahamstown (South Africa) -- History
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2556 , http://hdl.handle.net/10962/d1002409 , Grahamstown (South Africa) -- History
- Description: [From the Preface]: A Study in Local History: Grahamstown 1883-1904 aims to draw into a coherent picture the threads of political attitudes, approaches to racial issues and changes confronting the late Victorians in Grahamstown, particularly in the areas of sanitation, public health and shifting commercial frontiers. The relation of local development to national affairs has been investigated, although attempts to define exactly how the former influenced the latter, and vice versa, would involve one in the proverbial chicken-and-egg syndrome. Let it suffice to say that an understanding of events in the microcosm, or locality, lends clarity to the cross-current of affairs at the national level. It begins at the point where Grahamstown's commercial importance in the Eastern Cape declines after a flourish of hopes in the prospects of the Port Alfred harbour as a means of bringing trade back to Grahamstown. The study concludes when the foundation of Rhodes University College in 1904, provides a new centre of development for the city.
- Full Text:
- Date Issued: 1983
A study of the educational thought of Rudolf Steiner
- Authors: Blunt, Richard John Scawen
- Date: 1983
- Subjects: Steiner, Rudolf, 1861-1925 Education -- Philosophy
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1886 , http://hdl.handle.net/10962/d1005932
- Full Text:
- Date Issued: 1983
- Authors: Blunt, Richard John Scawen
- Date: 1983
- Subjects: Steiner, Rudolf, 1861-1925 Education -- Philosophy
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1886 , http://hdl.handle.net/10962/d1005932
- Full Text:
- Date Issued: 1983
A victimological study among Coloureds in the Cape Peninsula
- Authors: Strijdom, Hendrik Gert
- Date: 1983
- Subjects: Victims of crimes -- South Africa -- Western Cape Colored people (South Africa) -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3342 , http://hdl.handle.net/10962/d1004582
- Description: From Introduction: Criminology developed as a reaction to the various revolutions that were occurring in the European countries during the eighteenth and nineteenth centuries. In response to the turmoil and disorder of Western society criminologists attempted to discover the natural laws of society hoping to establish a stable social order. Crime was regarded as something that disturbed society and, therefore, had to be controlled or prevented. According to Quinney and Wildeman (1977) the development of criminology can be viewed as an ongoing attempt to explain crime in terms of established social order. They state that in the history of criminology there is, however, a lack of a clear accumulative theoretical growth and continue as follows: "No line of theoretical development can be found that leads to a well-developed body of knowledge. The study of crime is characterized by a number of divergent theoretical perspectives that exist in relative isolation from one another" (p. 38). Quinney and Wildeman (1977) distinguish five theoretical perspectives in the development of criminology namely: (I) early and classical criminological thought, (2) nineteenth-century sociological criminology, (3) nineteenth-century biological criminology, (4) twentieth-century eclectic criminology, and (5) twentieth-century sociological criminology.
- Full Text:
- Date Issued: 1983
- Authors: Strijdom, Hendrik Gert
- Date: 1983
- Subjects: Victims of crimes -- South Africa -- Western Cape Colored people (South Africa) -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3342 , http://hdl.handle.net/10962/d1004582
- Description: From Introduction: Criminology developed as a reaction to the various revolutions that were occurring in the European countries during the eighteenth and nineteenth centuries. In response to the turmoil and disorder of Western society criminologists attempted to discover the natural laws of society hoping to establish a stable social order. Crime was regarded as something that disturbed society and, therefore, had to be controlled or prevented. According to Quinney and Wildeman (1977) the development of criminology can be viewed as an ongoing attempt to explain crime in terms of established social order. They state that in the history of criminology there is, however, a lack of a clear accumulative theoretical growth and continue as follows: "No line of theoretical development can be found that leads to a well-developed body of knowledge. The study of crime is characterized by a number of divergent theoretical perspectives that exist in relative isolation from one another" (p. 38). Quinney and Wildeman (1977) distinguish five theoretical perspectives in the development of criminology namely: (I) early and classical criminological thought, (2) nineteenth-century sociological criminology, (3) nineteenth-century biological criminology, (4) twentieth-century eclectic criminology, and (5) twentieth-century sociological criminology.
- Full Text:
- Date Issued: 1983
Alcoholic adaptation : a preliminary investigation of the transactional analysis viewpoint, with application to delta and gamma alcoholics
- Authors: Cohen, L Derek
- Date: 1983
- Subjects: Alcoholism Alcoholism -- Case studies
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3193 , http://hdl.handle.net/10962/d1008559
- Description: Two delta and two gamma alcoholics were assessed by case study according to the theoretical formulations of Transactional Analysis. Scripts and Games were elicited through the Thematic Apperception Test, Laddering Procedure, and Life History. Analyses demonstrated, firstly, that parallels were present between the parent-child relationship and present adult transactions; secondly, that needs, fears, and control mechanisms were traceable to early parental injunctions; thirdly, that among these subjects, delta alcoholics tend to play the alcoholic game "Lush", and gamma alcoholics tend to play the alcoholic game "Drunk and Proud". It was concluded that the script and existential position appear to play an important role in the maintenance of the drinking pattern. Alcoholic Loss of Control appears to be influenced by the degree to which aggression is suppressed.
- Full Text:
- Date Issued: 1983
- Authors: Cohen, L Derek
- Date: 1983
- Subjects: Alcoholism Alcoholism -- Case studies
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3193 , http://hdl.handle.net/10962/d1008559
- Description: Two delta and two gamma alcoholics were assessed by case study according to the theoretical formulations of Transactional Analysis. Scripts and Games were elicited through the Thematic Apperception Test, Laddering Procedure, and Life History. Analyses demonstrated, firstly, that parallels were present between the parent-child relationship and present adult transactions; secondly, that needs, fears, and control mechanisms were traceable to early parental injunctions; thirdly, that among these subjects, delta alcoholics tend to play the alcoholic game "Lush", and gamma alcoholics tend to play the alcoholic game "Drunk and Proud". It was concluded that the script and existential position appear to play an important role in the maintenance of the drinking pattern. Alcoholic Loss of Control appears to be influenced by the degree to which aggression is suppressed.
- Full Text:
- Date Issued: 1983
An assessment of employment opportunities created by the Keiskamma Irrigation Scheme
- Authors: Nightingale, Fiona Mary
- Date: 1983
- Subjects: Economic development projects -- South Africa --Ciskei , Job creation -- South Africa -- Ciskei , Irrigation -- South Africa -- Ciskei , Agriculture -- Economic aspects -- South Africa -- Ciskei , Black people -- Employment -- South Africa -- Ciskei
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:4862 , http://hdl.handle.net/10962/d1006912 , Economic development projects -- South Africa --Ciskei , Job creation -- South Africa -- Ciskei , Irrigation -- South Africa -- Ciskei , Agriculture -- Economic aspects -- South Africa -- Ciskei , Black people -- Employment -- South Africa -- Ciskei
- Description: Preface: "The need for development as observed today is a problem peculiar to the 20th century. The current situation is characterised by the fact that there are no more empty places on the earth, while our use of dwindling resources is curbed by environmental constraint." (Mouly and Costa, 1974, 155). The need for development is not equally felt by all nations as disparities in income and standards of living on a national basis have dissected the world into developed and underdeveloped countries. Disparities also exist within nations. These divisions are not simply determined nor are the terms related to development clearly defined - a matter that will be discussed in Chapter Two. Impetus to developing underdeveloped countries may be provided by various means, such as a general injection of capital or by more specific development projects. The purpose of these projects is to initiate changes that are intended to promote rapid development of an area. An interest has been shown by Geographers in the ability of these projects to stimulate economic growth in surrounding areas (Silberfein, 1976). The resulting success of development projects is closely related, if not determined, in large measure, by the aims. In view of the need for the aims of development projects to be successfully fulfilled, research into what constitute constructive developmental aims would be beneficial. Previous studies have revealed problems encountered with conflicting aims and objectives (Mountjoy, 1971); poorly defined aims (de Wilde, 1967); and inadequate groundwork prior to initiating the project (Berry, 1976). It is the intention of the study to examine the impact made by a development project in an underdeveloped area, and thereby assess the extent to which the development aims have been carried out. In order to carry out an examination of a development project in an underdeveloped area, an area in need of development had to be selected; a project within the area chosen for study; and the particular aim of the project examined. The Ciskei, a Homeland in South Africa, was chosen as a suitable area for study. A number of factors contribute towards it being an area in need of development, particularly as the Ciskei is a small country and dependent on South Africa, which is explained in the overview of the Ciskei in Chapter One. Three characteristics of underdevelopment noted by Seers (1972) are poverty, inequality and underemployment. Of these three factors it was decided to focus on unemployment as the indication of the extent to which development has taken place. The reduction of unemployment was one of the aims of the Keiskammahoek Irrigation Scheme, the project chosen for study. The overview concludes with a presentation of the Keiskamma Irrigation Scheme.
- Full Text:
- Date Issued: 1983
- Authors: Nightingale, Fiona Mary
- Date: 1983
- Subjects: Economic development projects -- South Africa --Ciskei , Job creation -- South Africa -- Ciskei , Irrigation -- South Africa -- Ciskei , Agriculture -- Economic aspects -- South Africa -- Ciskei , Black people -- Employment -- South Africa -- Ciskei
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:4862 , http://hdl.handle.net/10962/d1006912 , Economic development projects -- South Africa --Ciskei , Job creation -- South Africa -- Ciskei , Irrigation -- South Africa -- Ciskei , Agriculture -- Economic aspects -- South Africa -- Ciskei , Black people -- Employment -- South Africa -- Ciskei
- Description: Preface: "The need for development as observed today is a problem peculiar to the 20th century. The current situation is characterised by the fact that there are no more empty places on the earth, while our use of dwindling resources is curbed by environmental constraint." (Mouly and Costa, 1974, 155). The need for development is not equally felt by all nations as disparities in income and standards of living on a national basis have dissected the world into developed and underdeveloped countries. Disparities also exist within nations. These divisions are not simply determined nor are the terms related to development clearly defined - a matter that will be discussed in Chapter Two. Impetus to developing underdeveloped countries may be provided by various means, such as a general injection of capital or by more specific development projects. The purpose of these projects is to initiate changes that are intended to promote rapid development of an area. An interest has been shown by Geographers in the ability of these projects to stimulate economic growth in surrounding areas (Silberfein, 1976). The resulting success of development projects is closely related, if not determined, in large measure, by the aims. In view of the need for the aims of development projects to be successfully fulfilled, research into what constitute constructive developmental aims would be beneficial. Previous studies have revealed problems encountered with conflicting aims and objectives (Mountjoy, 1971); poorly defined aims (de Wilde, 1967); and inadequate groundwork prior to initiating the project (Berry, 1976). It is the intention of the study to examine the impact made by a development project in an underdeveloped area, and thereby assess the extent to which the development aims have been carried out. In order to carry out an examination of a development project in an underdeveloped area, an area in need of development had to be selected; a project within the area chosen for study; and the particular aim of the project examined. The Ciskei, a Homeland in South Africa, was chosen as a suitable area for study. A number of factors contribute towards it being an area in need of development, particularly as the Ciskei is a small country and dependent on South Africa, which is explained in the overview of the Ciskei in Chapter One. Three characteristics of underdevelopment noted by Seers (1972) are poverty, inequality and underemployment. Of these three factors it was decided to focus on unemployment as the indication of the extent to which development has taken place. The reduction of unemployment was one of the aims of the Keiskammahoek Irrigation Scheme, the project chosen for study. The overview concludes with a presentation of the Keiskamma Irrigation Scheme.
- Full Text:
- Date Issued: 1983
An ecological study of the ixodid tick rhipicephalus glabroscutatum (Du Toit, 1941)
- Macivor, Keith Malcolm de Falloux
- Authors: Macivor, Keith Malcolm de Falloux
- Date: 1983
- Subjects: Ticks -- Control -- South Africa -- Eastern Cape Parasites -- South Africa -- Eastern Cape Rhipicephalus -- South Africa -- Eastern Cape Angora goat -- South Africa -- Eastern Cape Larvae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5602 , http://hdl.handle.net/10962/d1002052
- Description: An intensive study was conducted on the free-living larvae and parasitic life stages of R.glabroscutatum on Angora and Boer goats in the Uitenhage district. Free-living larvae exhibited periods of maximum relative abundance during months of generally lower rainfall, from the end of autumn to the beginning of spring. Relative larval abundance in open and gully sites was low while larvae were rarely found in bush sites. Relative abundance was higher at 20h00 than at 08h00 and 14h00. Repeated sampling in the same areas did not reduce larval numbers. Over 99% of parasitic ticks removed from Angora and Boer goats were located on the feet. The annual occurrence of a low and variable number of parasitic immatures was synchronous with the occurrence of free-living larvae. High, less variable numbers of adult ticks were removed from goat feet from September to December, the highest numbers being recorded during October and November. The occurrence of a single period of parasitic adult abundance annually indicated a life cycle with 1 generation per anum. There were higher numbers of adult ticks on the feet of the Angora goats than on the Boer goats. Adult numbers were higher on the hind feet of both breeds of goats than on the front feet. R.glabroscutatum adults seem to be implicated in the aetiology of foot abscesses since more infections were observed in Angora goats than in Boer goats and on the hind feet rather than the front feet within both goat groups. In addition R.glabroscutatum adults attached at interdigital sites where abscesses also originated and reached highest numbers in October and November when abscesses were most frequently observed. A review of distribution and host records indicated that R.glabroscutatum was limited in its distribution to the eastern Cape Province, being primarily located in South Africa's major mohair producing areas. R.glabroscutatum was classed an obligative xerophile on the basis of its recorded occurrence in non coastal areas with a low annual rainfall and Karoo and Karroid Bush vegetation. Host records for R.glabroscutatum included 10 species of wild ungulate, sheep, goats and cattle. The common site of occurrence on small stock and on small to medium sized wild animals appeared to be the legs and feet.
- Full Text:
- Date Issued: 1983
- Authors: Macivor, Keith Malcolm de Falloux
- Date: 1983
- Subjects: Ticks -- Control -- South Africa -- Eastern Cape Parasites -- South Africa -- Eastern Cape Rhipicephalus -- South Africa -- Eastern Cape Angora goat -- South Africa -- Eastern Cape Larvae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5602 , http://hdl.handle.net/10962/d1002052
- Description: An intensive study was conducted on the free-living larvae and parasitic life stages of R.glabroscutatum on Angora and Boer goats in the Uitenhage district. Free-living larvae exhibited periods of maximum relative abundance during months of generally lower rainfall, from the end of autumn to the beginning of spring. Relative larval abundance in open and gully sites was low while larvae were rarely found in bush sites. Relative abundance was higher at 20h00 than at 08h00 and 14h00. Repeated sampling in the same areas did not reduce larval numbers. Over 99% of parasitic ticks removed from Angora and Boer goats were located on the feet. The annual occurrence of a low and variable number of parasitic immatures was synchronous with the occurrence of free-living larvae. High, less variable numbers of adult ticks were removed from goat feet from September to December, the highest numbers being recorded during October and November. The occurrence of a single period of parasitic adult abundance annually indicated a life cycle with 1 generation per anum. There were higher numbers of adult ticks on the feet of the Angora goats than on the Boer goats. Adult numbers were higher on the hind feet of both breeds of goats than on the front feet. R.glabroscutatum adults seem to be implicated in the aetiology of foot abscesses since more infections were observed in Angora goats than in Boer goats and on the hind feet rather than the front feet within both goat groups. In addition R.glabroscutatum adults attached at interdigital sites where abscesses also originated and reached highest numbers in October and November when abscesses were most frequently observed. A review of distribution and host records indicated that R.glabroscutatum was limited in its distribution to the eastern Cape Province, being primarily located in South Africa's major mohair producing areas. R.glabroscutatum was classed an obligative xerophile on the basis of its recorded occurrence in non coastal areas with a low annual rainfall and Karoo and Karroid Bush vegetation. Host records for R.glabroscutatum included 10 species of wild ungulate, sheep, goats and cattle. The common site of occurrence on small stock and on small to medium sized wild animals appeared to be the legs and feet.
- Full Text:
- Date Issued: 1983
An investigation into the problems related to the adjustment of beginning-teachers leading to the development of an induction programme
- Authors: Algie, Rowan Craig
- Date: 1983
- Subjects: Teachers, Probationary -- South Africa Teaching -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1411 , http://hdl.handle.net/10962/d1003074
- Description: There is little doubt that there is a widespread concern that the transition from the supportive environment of the training institution where mistakes are expected, self criticism is encouraged and both tutorial guidance and peer group friendship are readily available into a situation where professional and personal responsibilities are profoundly altered, where the methods used, the progress of the pupils, their parents' expectations, the working relationship with colleagues are now permanent responsibilities. This transition can be traumatic for some and a considerable ordeal for many.--Chapter 1, p. 10.
- Full Text:
- Date Issued: 1983
- Authors: Algie, Rowan Craig
- Date: 1983
- Subjects: Teachers, Probationary -- South Africa Teaching -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1411 , http://hdl.handle.net/10962/d1003074
- Description: There is little doubt that there is a widespread concern that the transition from the supportive environment of the training institution where mistakes are expected, self criticism is encouraged and both tutorial guidance and peer group friendship are readily available into a situation where professional and personal responsibilities are profoundly altered, where the methods used, the progress of the pupils, their parents' expectations, the working relationship with colleagues are now permanent responsibilities. This transition can be traumatic for some and a considerable ordeal for many.--Chapter 1, p. 10.
- Full Text:
- Date Issued: 1983
An investigation of the shared experience of children's literature, with particular reference to the teacher reading aloud, in senior primary classes
- Authors: McKellar, David William
- Date: 1983
- Subjects: Reading (Elementary) -- South Africa Teachers -- Training of -- South Africa Children's literature Children -- Books and reading
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1919 , http://hdl.handle.net/10962/d1007401
- Full Text:
- Date Issued: 1983
- Authors: McKellar, David William
- Date: 1983
- Subjects: Reading (Elementary) -- South Africa Teachers -- Training of -- South Africa Children's literature Children -- Books and reading
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1919 , http://hdl.handle.net/10962/d1007401
- Full Text:
- Date Issued: 1983
Aspects of prostacyclin in experimental hypertension
- Authors: Botha, Julia Hilary
- Date: 1983
- Subjects: Prostacyclin Prostaglandins Prostaglandin endoperoxides Thromboxanes Hypertension
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3825 , http://hdl.handle.net/10962/d1006109
- Description: A new prostaglandin - prostaglandin X (later renamed prostacyclin or prostaglandin I₂ (PGI₂)), was discovered by Moncada, Gryglewski, Bunting and Vane in 1976. This unstable substance was shown to be produced by vascular tissue and to be a vasodilator and the most potent endogenous inhibitor of platelet aggregation known. Because of its properties, it appeared that a lack of it may be related to the development and or maintenance of hypertension, a disorder featuring vasoconstriction and an increased tendency to arterial thrombosis. The present studies aimed to investigate this possibility using a rat model. A bioassay for prostacyclin was first perfected. This consisted of a modification of the method used by Moncada, Higgs and Vane (1977): PGI₂ released by rat aortic strips, during incubation in tris buffer, was measured by assessing the ability of the incubate to inhibit adenosine diphosphate induced aggregation of human platelets, as compared to the inhibitory effect of standard prostacyclin sodium salt. The specificity of the assay for the detection of PGI₂ was tested. The abil ity of hypertensive rat aorta to release prostacycl in was investigated in two studies. The first compared aortas of Wistar rats of the New Zealand genetically hypertensive strain (GH) with those of matched normotensive Wistar controls. In the second study, hypertension was induced by wrappi ng the ri ght kidney with surgical silk and removing the contralateral kidney. Ten weeks later, aortic generation of prostacyclin by these animals was compared with that of matched sham controls which had received identical surgical manipulation but for the application of silk to the right kidney. Contrary to expectation, in both forms of hypertension, aortas of the rats with elevated pressure produced consistently more prostacyclin than those of matched controls. In order to discover more about the relationship between elevated pressure and elevated PGI₂ production, the effect of pressure reduction with hypotensive agents on the ability of GH rat aortas to produce prostacyclin, was investigated. After pressure had been controlled within normal range for one week (achieved by oral administration of furosemide, dihydralazine and reserpine for one month), aortic PGI₂ was reduced in comparison with matched GH controls. However, the reduction was not consistent and statistical significance was not reached. Because it was subsequently reported by other workers, that some of the hypotensive agents which had been employed may effect prostaglandin levels per se, no conclusions could be drawn from this study as to any possible direct relationships between pressure and aortic prostacyclin generating capacity. A further means of reducing elevated pressure (which had no inherent effect on prostaglandin levels) was thus sought. A mechanical method was eventually selected, application of a silver clip to the aortas of GH rats, just below the diaphragm, producing an immediate reduction in pressure distal to the constriction. Eighteen hours with later, PGI₂ production by these distal aortas those of matched sham GH controls and was was compared found to be consistently reduced. These results indicate that the ability to produce PGI₂ may be influenced by prior local pressure changes and that the increased capacity of hypertensive rat aortas to generate prostacyclin may be related to the increased mechanical transmural stress consequent on elevated pressure. Since haemostatic balance must be influenced not only by vascular PGI₂ generation but also by platelet sensitivity to PGI₂, the response of GH platelets to the anti-aggregatory effect of prostacyc1in was also investigated. As it had been shown by Sinzinger, Si1berbauer, Horsch and Gall (1981) that intra-arterial infusion of PGI₂ in humans decreased platelet sensitivity to the substance, the possibility existed that platelet sensitivity in hypertension might be reduced. This hypothesis was, however, invalidated as the sensitivity of GH platelets to the anti-aggregatory effect of PGI₂ was almost identical to that of normotensive controls. The shortcomings of the methodology and the possible importance of these findings in the hypertensive animal are discussed. The idea that elevated PGI₂ in hypertension may play a protective role both with respect to platelet aggregation and in attenuating further pressure rises is considered. It is finally suggested that it will be possible to draw more accurate conclusions as to the meaning of the increased PGI₂ generation in hypertension (both in relation to vascular tone and platelet function) only when details of production of, and sensitivity to, thromboxane A₂ are known. Thromboxane A₂ (TXA₂) is a vasoconstrictor and promotor of aggregation (Hamberg, Svensson and Samuelson, 1975) and it may be that, despite elevated vascular PGI₂ generation, the TXA₂/PGI₂ balance is still tipped in favour of vasoconstriction and platelet aggregation in hypertension.
- Full Text:
- Date Issued: 1983
- Authors: Botha, Julia Hilary
- Date: 1983
- Subjects: Prostacyclin Prostaglandins Prostaglandin endoperoxides Thromboxanes Hypertension
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3825 , http://hdl.handle.net/10962/d1006109
- Description: A new prostaglandin - prostaglandin X (later renamed prostacyclin or prostaglandin I₂ (PGI₂)), was discovered by Moncada, Gryglewski, Bunting and Vane in 1976. This unstable substance was shown to be produced by vascular tissue and to be a vasodilator and the most potent endogenous inhibitor of platelet aggregation known. Because of its properties, it appeared that a lack of it may be related to the development and or maintenance of hypertension, a disorder featuring vasoconstriction and an increased tendency to arterial thrombosis. The present studies aimed to investigate this possibility using a rat model. A bioassay for prostacyclin was first perfected. This consisted of a modification of the method used by Moncada, Higgs and Vane (1977): PGI₂ released by rat aortic strips, during incubation in tris buffer, was measured by assessing the ability of the incubate to inhibit adenosine diphosphate induced aggregation of human platelets, as compared to the inhibitory effect of standard prostacyclin sodium salt. The specificity of the assay for the detection of PGI₂ was tested. The abil ity of hypertensive rat aorta to release prostacycl in was investigated in two studies. The first compared aortas of Wistar rats of the New Zealand genetically hypertensive strain (GH) with those of matched normotensive Wistar controls. In the second study, hypertension was induced by wrappi ng the ri ght kidney with surgical silk and removing the contralateral kidney. Ten weeks later, aortic generation of prostacyclin by these animals was compared with that of matched sham controls which had received identical surgical manipulation but for the application of silk to the right kidney. Contrary to expectation, in both forms of hypertension, aortas of the rats with elevated pressure produced consistently more prostacyclin than those of matched controls. In order to discover more about the relationship between elevated pressure and elevated PGI₂ production, the effect of pressure reduction with hypotensive agents on the ability of GH rat aortas to produce prostacyclin, was investigated. After pressure had been controlled within normal range for one week (achieved by oral administration of furosemide, dihydralazine and reserpine for one month), aortic PGI₂ was reduced in comparison with matched GH controls. However, the reduction was not consistent and statistical significance was not reached. Because it was subsequently reported by other workers, that some of the hypotensive agents which had been employed may effect prostaglandin levels per se, no conclusions could be drawn from this study as to any possible direct relationships between pressure and aortic prostacyclin generating capacity. A further means of reducing elevated pressure (which had no inherent effect on prostaglandin levels) was thus sought. A mechanical method was eventually selected, application of a silver clip to the aortas of GH rats, just below the diaphragm, producing an immediate reduction in pressure distal to the constriction. Eighteen hours with later, PGI₂ production by these distal aortas those of matched sham GH controls and was was compared found to be consistently reduced. These results indicate that the ability to produce PGI₂ may be influenced by prior local pressure changes and that the increased capacity of hypertensive rat aortas to generate prostacyclin may be related to the increased mechanical transmural stress consequent on elevated pressure. Since haemostatic balance must be influenced not only by vascular PGI₂ generation but also by platelet sensitivity to PGI₂, the response of GH platelets to the anti-aggregatory effect of prostacyc1in was also investigated. As it had been shown by Sinzinger, Si1berbauer, Horsch and Gall (1981) that intra-arterial infusion of PGI₂ in humans decreased platelet sensitivity to the substance, the possibility existed that platelet sensitivity in hypertension might be reduced. This hypothesis was, however, invalidated as the sensitivity of GH platelets to the anti-aggregatory effect of PGI₂ was almost identical to that of normotensive controls. The shortcomings of the methodology and the possible importance of these findings in the hypertensive animal are discussed. The idea that elevated PGI₂ in hypertension may play a protective role both with respect to platelet aggregation and in attenuating further pressure rises is considered. It is finally suggested that it will be possible to draw more accurate conclusions as to the meaning of the increased PGI₂ generation in hypertension (both in relation to vascular tone and platelet function) only when details of production of, and sensitivity to, thromboxane A₂ are known. Thromboxane A₂ (TXA₂) is a vasoconstrictor and promotor of aggregation (Hamberg, Svensson and Samuelson, 1975) and it may be that, despite elevated vascular PGI₂ generation, the TXA₂/PGI₂ balance is still tipped in favour of vasoconstriction and platelet aggregation in hypertension.
- Full Text:
- Date Issued: 1983
Behavioural methods for the control of examination anxiety : an experimental investigation
- Authors: Norton, Gary Kenneth
- Date: 1983
- Subjects: Anxiety , Test anxiety
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2916 , http://hdl.handle.net/10962/d1002081
- Description: In 1982, it was reported that one in every three students who fail at South African universities, do not pass because of experiences of excessive anxiety resulting from university examinations. A survey conducted amongst student counsellors and counselling services on all South African university campuses, revealed a deficiency of group and individual therapy for this phenomenon of examination anxiety. The cause of this deficiency, was found to be the already excessive demands made on the time of student counsellors. Noting a similar situation at Rhodes University, the present investigation was initiated, with the aim of developing an economical group counselling programme for test anxious students on Rhodes campus. Sixty-four Rhodes students (who identified themselves as test anxious) volunteered for this investigation. These Subjects were randomly assigned to one of four treatment programmes (each divided into two groups, where Group A, N=6 and Group B, N=7) and a wait-list control group (N=12). Three of the treatments featured multicomponent programmes, each offering a cluster of behavioural interventions centred around: Study Skills Training/Counselling; Systematic Desensitisation (Using individually-constructed anxiety hierarchies); Systematic Desensitisation (Using group-constructed anxiety hierarchies). Included in addition, was a single-component treatment, featuring cognitive modification: a component much favoured by local test anxiety counsellors. Given adverse reports concerning the efficacy of single component programmes, when contrasted with multi component treatments, the cognitive modification package was included as a placebo. A battery of measures was used to assess test anxiety and progress made by Subjects to assuage its debilitative effects . The measures included: (a) Six Self-report measures (including a treatment evaluation schedule and the maintenance of a diary of experience by each Subject); (b) Two measures of physiological reactivity, viz. pulse rate and finger sweat print; and (c) One 'observable' measure, that of academic performance. Using these measures, an assessment of the performance gains or losses by each of the Subjects, was made on three occasions: at pre-treatment, post-treatment and 6-month follow-up. In support of the a priori hypotheses, the systematic desensitisation and study skills multicomponent programmes, realised significant gains on all measures over both the placebo and control groups (with exception of the higher score achieved by placebo subjects over that of the study skills group, on the treatment evaluation schedule) . This finding confirmed the superiority of multicomponent programmes over those with only a single component. The systematic desensitisation programmes proved to be the most effective, as measured on self-report and physiological measures. The superiority of group-constructed anxiety hierarchies over those individually-constructed was established. Study skills training helped Subjects to realise and maintain gains on the academic performance measure, although it took many of these subjects six months, before they had fully incorporated the study techniques taught, in with their own study habits. In discussion of the findings, the evident need to match test anxious students to programmes which "best suit" their characteristics, is presented, and solutions proposed. Weaknesses evident in the measuring instruments and research design, are also highlighted for discussion. As part of this experimental investigation, a discussion on the nature of test anxiety and its links with anxiety theory is introduced, together with a review of problems in measuring test anxiety; popular behavioural treatments used to relieve test anxiety; and a survey of test anxiety counselling on South African campuses. Advice for the therapist/counsellor, the academic, and the researcher, are posited in conclusion.
- Full Text:
- Date Issued: 1983
- Authors: Norton, Gary Kenneth
- Date: 1983
- Subjects: Anxiety , Test anxiety
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2916 , http://hdl.handle.net/10962/d1002081
- Description: In 1982, it was reported that one in every three students who fail at South African universities, do not pass because of experiences of excessive anxiety resulting from university examinations. A survey conducted amongst student counsellors and counselling services on all South African university campuses, revealed a deficiency of group and individual therapy for this phenomenon of examination anxiety. The cause of this deficiency, was found to be the already excessive demands made on the time of student counsellors. Noting a similar situation at Rhodes University, the present investigation was initiated, with the aim of developing an economical group counselling programme for test anxious students on Rhodes campus. Sixty-four Rhodes students (who identified themselves as test anxious) volunteered for this investigation. These Subjects were randomly assigned to one of four treatment programmes (each divided into two groups, where Group A, N=6 and Group B, N=7) and a wait-list control group (N=12). Three of the treatments featured multicomponent programmes, each offering a cluster of behavioural interventions centred around: Study Skills Training/Counselling; Systematic Desensitisation (Using individually-constructed anxiety hierarchies); Systematic Desensitisation (Using group-constructed anxiety hierarchies). Included in addition, was a single-component treatment, featuring cognitive modification: a component much favoured by local test anxiety counsellors. Given adverse reports concerning the efficacy of single component programmes, when contrasted with multi component treatments, the cognitive modification package was included as a placebo. A battery of measures was used to assess test anxiety and progress made by Subjects to assuage its debilitative effects . The measures included: (a) Six Self-report measures (including a treatment evaluation schedule and the maintenance of a diary of experience by each Subject); (b) Two measures of physiological reactivity, viz. pulse rate and finger sweat print; and (c) One 'observable' measure, that of academic performance. Using these measures, an assessment of the performance gains or losses by each of the Subjects, was made on three occasions: at pre-treatment, post-treatment and 6-month follow-up. In support of the a priori hypotheses, the systematic desensitisation and study skills multicomponent programmes, realised significant gains on all measures over both the placebo and control groups (with exception of the higher score achieved by placebo subjects over that of the study skills group, on the treatment evaluation schedule) . This finding confirmed the superiority of multicomponent programmes over those with only a single component. The systematic desensitisation programmes proved to be the most effective, as measured on self-report and physiological measures. The superiority of group-constructed anxiety hierarchies over those individually-constructed was established. Study skills training helped Subjects to realise and maintain gains on the academic performance measure, although it took many of these subjects six months, before they had fully incorporated the study techniques taught, in with their own study habits. In discussion of the findings, the evident need to match test anxious students to programmes which "best suit" their characteristics, is presented, and solutions proposed. Weaknesses evident in the measuring instruments and research design, are also highlighted for discussion. As part of this experimental investigation, a discussion on the nature of test anxiety and its links with anxiety theory is introduced, together with a review of problems in measuring test anxiety; popular behavioural treatments used to relieve test anxiety; and a survey of test anxiety counselling on South African campuses. Advice for the therapist/counsellor, the academic, and the researcher, are posited in conclusion.
- Full Text:
- Date Issued: 1983
Cognitive structuring of residential environments in black Grahamstown: a political view
- Authors: Taylor, Beverley Mary King
- Date: 1983
- Subjects: Black people -- Housing -- South Africa -- Grahamstown -- Political aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:4809 , http://hdl.handle.net/10962/d1004546 , Black people -- Housing -- South Africa -- Grahamstown -- Political aspects
- Description: This research project investigates black cognitive structuring of their residential environment in the Grahamstown location. A clinical psychological method (repertory grid method) was used to elicit the construct systems of residents. The associative construct theory formulated by Kelly (1955) was used in interpreting the data set from the liberal perspective. The radical perspective demonstrated an alternative interpretation. A focus of the study centres around the possible implications of this type of research for planning action. The results showed that the repertory grid did appear to accurately reflect people's construing systems regarding their circumstances and behaviour. However, Kelly's (1955) Personal Construct Theory proved inadequate as a theory of explanation as to why people construed in the manner they did. To enhance this explanation, the marxist approach to the theory of knowledge was investigated.
- Full Text:
- Date Issued: 1983
- Authors: Taylor, Beverley Mary King
- Date: 1983
- Subjects: Black people -- Housing -- South Africa -- Grahamstown -- Political aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:4809 , http://hdl.handle.net/10962/d1004546 , Black people -- Housing -- South Africa -- Grahamstown -- Political aspects
- Description: This research project investigates black cognitive structuring of their residential environment in the Grahamstown location. A clinical psychological method (repertory grid method) was used to elicit the construct systems of residents. The associative construct theory formulated by Kelly (1955) was used in interpreting the data set from the liberal perspective. The radical perspective demonstrated an alternative interpretation. A focus of the study centres around the possible implications of this type of research for planning action. The results showed that the repertory grid did appear to accurately reflect people's construing systems regarding their circumstances and behaviour. However, Kelly's (1955) Personal Construct Theory proved inadequate as a theory of explanation as to why people construed in the manner they did. To enhance this explanation, the marxist approach to the theory of knowledge was investigated.
- Full Text:
- Date Issued: 1983
Conductivity profiles for a horizontally uniform earth
- Authors: Murrell, Hugh Crozier
- Date: 1983
- Subjects: Coen, Shimon -- Criticism and interpretation Wang-Ho Yu, Michael -- Criticism and interpretation Algorithms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5395 , http://hdl.handle.net/10962/d1001984
- Description: An investigation is made into the mathematics behind the noniterative inversion algorithm of Shimon Coen and Michael Wang-Ho Yu [1981]. The algorithm determines the conductivity profile of a horizontally uniform earth from surface measurements of apparent resistivity with a Schlumberger array. The algorithm is checked by performing the inversion on both artifical and raw field data
- Full Text:
- Date Issued: 1983
- Authors: Murrell, Hugh Crozier
- Date: 1983
- Subjects: Coen, Shimon -- Criticism and interpretation Wang-Ho Yu, Michael -- Criticism and interpretation Algorithms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5395 , http://hdl.handle.net/10962/d1001984
- Description: An investigation is made into the mathematics behind the noniterative inversion algorithm of Shimon Coen and Michael Wang-Ho Yu [1981]. The algorithm determines the conductivity profile of a horizontally uniform earth from surface measurements of apparent resistivity with a Schlumberger array. The algorithm is checked by performing the inversion on both artifical and raw field data
- Full Text:
- Date Issued: 1983
Continuous-flow dynamic dialysis and its application to collagen-ligand interactions
- Authors: Sparrow, Neil Arthur
- Date: 1983
- Subjects: Collagen Ligands (Biochemistry) Ligand binding (Biochemistry) Protein-protein interactions Tannins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4297 , http://hdl.handle.net/10962/d1004617
- Description: Studies undertaken to investigate the binding of low molecular mass analogues of polyphenolic vegetable tannins to collagen have prompted the development of a new method to investigate protein-ligand interactions. This method, the continuous-flow dynamic dialysis method (CFDD), differs from conventional dialysis procedures used for protein-ligand binding studies. In this method, the ligand concentration in the diffusate is monitored automatically at successive closely spaced time intervals while being continuously eluted from the dialysis cell. The primary data obtained by this method consists of a series of spectrophotometric absorbance measurements representing the ligand concentration in the sink compartment of a dialysis cell. This primary data is recorded by means of a data logging device onto a punched paper tape for subsequent computer processing. Two original methods are presented for analysing the primary data to extract the protein-ligand binding isotherm. The first of these is a direct analysis which relies on Fick's first law of diffusion. In this method it is necessary to establish, by means of a control experiment, a value for the ligand permeation constant. This is used in a subsequent analysis to establish a relationship between the measured rate of diffusion of the ligand from a protein-ligand mixture and the concentration of unbound ligand which is in equilibrium with the protein-ligand complex. The protein-ligand binding isotherm is obtained from parametric equations which give the quantity of ligand bound to the protein and the concentration of unbound ligand in the sample compartment as functions of time. The second method, which is more general, utilizes the same primary data but is based on establishing a system transfer function to characterise the dialysis and eluting processes. This analysis depends on the linearity of the system and utilizes numerical laplace transforms of the primary data sets obtained from control and protein-ligand dialyses. Laplace transforms are used to effect a deconvolution of the transfer function from the primary data and yield the concentration of ligand in equilibrium with the protein-ligand complex. This procedure yields, simultaneously, both the total ligand concentration and the concentration of unbound ligand in the protein compartment of the dialysis cell. These quantities are used to establish the binding isotherm for the protein ligand system. Numerical inversion of the laplace transforms in this analysis is effected by their reduction to Fourier series. The experimental reliability of the continuous-flow dynamic dialysis method, and validity of the two analytical methods used to derive a binding isotherm from dialysis data are evaluated from studies of the binding of phenol red to bovine serum albumin (BSA) at 15⁰, 20⁰ and 25⁰ C, as well as from simulated binding curves generated by the numerical solution of the differential equations used to describe the dialysis and elution process in terms of a two-site Scatchard binding model. The method is used to investigate the binding to collagen of a series of low molecular mass phenolic compounds which can be isolated from Wattle and Quebracho vegetable tannin extracts. These compounds can be considered as monomeric precursor analogues of the polymeric vegetable tannins. The binding of these ligands to collagen is shown to be characterised by high capacity, low affinity binding in which the uptake of ligand by the protein increases linearly with increasing ligand concentration. Collagen exhibits no indication of site saturation for these ligands over the experimentally accessible concentration ranges investigated.
- Full Text:
- Date Issued: 1983
- Authors: Sparrow, Neil Arthur
- Date: 1983
- Subjects: Collagen Ligands (Biochemistry) Ligand binding (Biochemistry) Protein-protein interactions Tannins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4297 , http://hdl.handle.net/10962/d1004617
- Description: Studies undertaken to investigate the binding of low molecular mass analogues of polyphenolic vegetable tannins to collagen have prompted the development of a new method to investigate protein-ligand interactions. This method, the continuous-flow dynamic dialysis method (CFDD), differs from conventional dialysis procedures used for protein-ligand binding studies. In this method, the ligand concentration in the diffusate is monitored automatically at successive closely spaced time intervals while being continuously eluted from the dialysis cell. The primary data obtained by this method consists of a series of spectrophotometric absorbance measurements representing the ligand concentration in the sink compartment of a dialysis cell. This primary data is recorded by means of a data logging device onto a punched paper tape for subsequent computer processing. Two original methods are presented for analysing the primary data to extract the protein-ligand binding isotherm. The first of these is a direct analysis which relies on Fick's first law of diffusion. In this method it is necessary to establish, by means of a control experiment, a value for the ligand permeation constant. This is used in a subsequent analysis to establish a relationship between the measured rate of diffusion of the ligand from a protein-ligand mixture and the concentration of unbound ligand which is in equilibrium with the protein-ligand complex. The protein-ligand binding isotherm is obtained from parametric equations which give the quantity of ligand bound to the protein and the concentration of unbound ligand in the sample compartment as functions of time. The second method, which is more general, utilizes the same primary data but is based on establishing a system transfer function to characterise the dialysis and eluting processes. This analysis depends on the linearity of the system and utilizes numerical laplace transforms of the primary data sets obtained from control and protein-ligand dialyses. Laplace transforms are used to effect a deconvolution of the transfer function from the primary data and yield the concentration of ligand in equilibrium with the protein-ligand complex. This procedure yields, simultaneously, both the total ligand concentration and the concentration of unbound ligand in the protein compartment of the dialysis cell. These quantities are used to establish the binding isotherm for the protein ligand system. Numerical inversion of the laplace transforms in this analysis is effected by their reduction to Fourier series. The experimental reliability of the continuous-flow dynamic dialysis method, and validity of the two analytical methods used to derive a binding isotherm from dialysis data are evaluated from studies of the binding of phenol red to bovine serum albumin (BSA) at 15⁰, 20⁰ and 25⁰ C, as well as from simulated binding curves generated by the numerical solution of the differential equations used to describe the dialysis and elution process in terms of a two-site Scatchard binding model. The method is used to investigate the binding to collagen of a series of low molecular mass phenolic compounds which can be isolated from Wattle and Quebracho vegetable tannin extracts. These compounds can be considered as monomeric precursor analogues of the polymeric vegetable tannins. The binding of these ligands to collagen is shown to be characterised by high capacity, low affinity binding in which the uptake of ligand by the protein increases linearly with increasing ligand concentration. Collagen exhibits no indication of site saturation for these ligands over the experimentally accessible concentration ranges investigated.
- Full Text:
- Date Issued: 1983
Corporate financial reporting: history, development and future directions
- Prinsloo, K S (Keith Stephen)
- Authors: Prinsloo, K S (Keith Stephen)
- Date: 1983
- Subjects: Corporations -- Finance , Financial statements , Accounting
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:902 , http://hdl.handle.net/10962/d1007055 , Corporations -- Finance , Financial statements , Accounting
- Description: KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1983
- Authors: Prinsloo, K S (Keith Stephen)
- Date: 1983
- Subjects: Corporations -- Finance , Financial statements , Accounting
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:902 , http://hdl.handle.net/10962/d1007055 , Corporations -- Finance , Financial statements , Accounting
- Description: KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1983
Economic geology of sulphide nickel deposits
- Authors: Harrison, P A
- Date: 1983
- Subjects: Nickel sulfide , Ore deposits , Geology, Economic , Silicate minerals , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5022 , http://hdl.handle.net/10962/d1006349
- Description: From Chapter 1: It has been a long standing belief that many nickel sulphide ores are derivatives of magmatic processes in ultramafic and mafic rocks, and that they segregate from these magmas as immiscible sulphide droplets which are then concentrated into an orebody by gravitational settling either during intrusion or extrusion, or during the early stages of crystallization of the magma (Naldrett, 1981). Some geologists however, have suggested alternative mechanisms to explain the concentration of nickeliferous sulphides in the mafic and ultramafic hosts. These include hydrothermal replacement (Fleet, 1977), exhalative volcanic processes (Lusk, 1976), or major metamorphic upgrading of low grade, initially magmatic deposits (Barrett et al., 1977). It is not the purpose of this study to verify or disprove these hypotheses, but in so far as the initial concentration of sulphides in most deposits is concerned, these effects are relatively unimportant (Naldrett, 1981). The nickel sulphide ores associated with these mafic and ultramafic host rocks, invariably consist of nickeliferous pyrrhotite as the dominant phase, together with lesser, but variable, amounts of magnetite, pentlandite, chalcopyrite, cubanite, and platinum group elements (Reynolds, 1982).
- Full Text:
- Date Issued: 1983
- Authors: Harrison, P A
- Date: 1983
- Subjects: Nickel sulfide , Ore deposits , Geology, Economic , Silicate minerals , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5022 , http://hdl.handle.net/10962/d1006349
- Description: From Chapter 1: It has been a long standing belief that many nickel sulphide ores are derivatives of magmatic processes in ultramafic and mafic rocks, and that they segregate from these magmas as immiscible sulphide droplets which are then concentrated into an orebody by gravitational settling either during intrusion or extrusion, or during the early stages of crystallization of the magma (Naldrett, 1981). Some geologists however, have suggested alternative mechanisms to explain the concentration of nickeliferous sulphides in the mafic and ultramafic hosts. These include hydrothermal replacement (Fleet, 1977), exhalative volcanic processes (Lusk, 1976), or major metamorphic upgrading of low grade, initially magmatic deposits (Barrett et al., 1977). It is not the purpose of this study to verify or disprove these hypotheses, but in so far as the initial concentration of sulphides in most deposits is concerned, these effects are relatively unimportant (Naldrett, 1981). The nickel sulphide ores associated with these mafic and ultramafic host rocks, invariably consist of nickeliferous pyrrhotite as the dominant phase, together with lesser, but variable, amounts of magnetite, pentlandite, chalcopyrite, cubanite, and platinum group elements (Reynolds, 1982).
- Full Text:
- Date Issued: 1983