Product development as part of a positioning strategy for the hunting industry in the Eastern Cape
- Authors: Van Niekerk, Pieter du Preez
- Date: 2002
- Subjects: New products -- Marketing , Hunting -- South Africa -- Eastern Cape , Game farms -- South Africa -- Eastern Cape , Venison -- Marketing , Meat industry and trade -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:10894 , http://hdl.handle.net/10948/111 , New products -- Marketing , Hunting -- South Africa -- Eastern Cape , Game farms -- South Africa -- Eastern Cape , Venison -- Marketing , Meat industry and trade -- South Africa -- Eastern Cape
- Description: In this study on product development and positioning in the hunting industry in the Eastern Cape, two conceptual frameworks were developed, based on a literature study. These positioning and product development concepts were tested against primary and secondary information gathered about the hunting industry. The game and hunting industries were in a strong growth phase at the time of this study. These industries are playing an increasingly important role in the economy of the Eastern Cape, and more and more agricultural land is utilised for game ranching. From the perspective of obtaining a competitive advantage in an increasingly competitive environment, it is therefore important for hunting providers to develop suitable products, and to position these products correctly. It is also important to investigate the characteristics of the game and hunting industries in the Eastern Cape and to get information about the characteristics of the market in which product development and positioning take place. Hunting is the most important form of game utilisation in the Eastern Cape, followed by cropping of game and live game sales. At the time of this study, the total value of the game utilised annually in the Eastern Cape was in excess of R168 million. Game utilisation seemed to be taking place on a sustainable basis, as the number of animals utilised annually was less than the annual production. Kudu and springbok provide the biggest total income, and, together with mountain reedbuck, are the most abundant species in the Eastern Cape. The main game-producing areas in the Eastern Cape coincide with the areas where extensive beef, sheep and goat farming are practised. The hunting products on offer from hunting providers, differ in certain ways. Aspects like price, type of hunting provided, game species available for hunting, terrain where hunting takes place, and various other aspects in connection with the hunt, differ from hunting provider to hunting provider. Characteristics of the hunting experience that hunters desire may be divided into core and variable elements. The core elements include: · the conditions pertaining to the hunt being spelt out clearly; · the maintenance of safety standards; · reasonable day fees; · the presence of game in sufficient numbers; and · other aspects generally desired by hunters. In the study it was found that the needs of hunters also differ regarding certain aspects of the hunting experience. These variable elements include price levels, type and availability of accommodation, and the role of the hunting provider. The core aspects that hunters desire are as a rule part of the product offering. From a product development perspective, the variable elements provide an opportunity to customise products to hunter needs. The inclusion or exclusion of variable elements from the product offering give rise to certain unique product features, and therefore provide the opportunity for product differentiation. After development, products are positioned firstly by selecting a market segment to focus on. Secondly, the needs of hunters in the target segment should be clearly identified, followed by the development of suitable products based on hunter needs, and taking into account the features of competitive offerings. Decisions should also be taken about the features to be included in the product offering to achieve product differentiation. After development of a suitable product, an image or brand should be chosen for the product, and this image should be effectively communicated to the market. A number of possible positioning options were identified for hunting providers. It was found that factors like the physical features of the ranch, available infrastructure, the market segment chosen, desires of the hunting providers, and the perception of the needs of hunters, eventually influence decisions about product development and positioning. The final decisions on positioning should take the core competencies of the hunting provider into account and should ensure that the hunting provider has the ability to fill the chosen position in the market.
- Full Text:
- Date Issued: 2002
RADGIS - an improved architecture for runtime-extensible, distributed GIS applications
- Authors: Preston, Richard Michael
- Date: 2002
- Subjects: Geographic information systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4626 , http://hdl.handle.net/10962/d1006497
- Description: A number of GIS architectures and technologies have emerged recently to facilitate the visualisation and processing of geospatial data over the Web. The work presented in this dissertation builds on these efforts and undertakes to overcome some of the major problems with traditional GIS client architectures, including application bloat, lack of customisability, and lack of interoperability between GIS products. In this dissertation we describe how a new client-side GIS architecture was developed and implemented as a proof-of-concept application called RADGIS, which is based on open standards and emerging distributed component-based software paradigms. RADGIS reflects the current trend in development focus from Web browser-based applications to customised clients, based on open standards, that make use of distributed Web services. While much attention has been paid to exposing data on the Web, there is growing momentum towards providing “value-added” services. A good example of this is the tremendous industry interest in the provision of location-based services, which has been discussed as a special use-case of our RADGIS architecture. Thus, in the near future client applications will not simply be used to access data transparently, but will also become facilitators for the location-transparent invocation of local and remote services. This flexible architecture will ensure that data can be stored and processed independently of the location of the client that wishes to view or interact with it. Our RADGIS application enables content developers and end-users to create and/or customise GIS applications dynamically at runtime through the incorporation of GIS services. This ensures that the client application has the flexibility to withstand changing levels of expertise or user requirements. These GIS services are implemented as components that execute locally on the client machine, or as remote CORBA Objects or EJBs. Assembly and deployment of these components is achieved using a specialised XML descriptor. This XML descriptor is written using a markup language that we developed specifically for this purpose, called DGCML, which contains deployment information, as well as a GUI specification and links to an XML-based help system that can be merged with the RADGIS client application’s existing help system. Thus, no additional requirements are imposed on object developers by the RADGIS architecture, i.e. there is no need to rewrite existing objects since DGCML acts as a runtime-customisable wrapper, allowing existing objects to be utilised by RADGIS. While the focus of this thesis has been on overcoming the above-mentioned problems with traditional GIS applications, the work described here can also be applied in a much broader context, especially in the development of highly customisable client applications that are able to integrate Web services at runtime.
- Full Text:
- Date Issued: 2002
Removal and recovery of gold and platinum from aqueous solutions utilising the non-viable biomass Asolla filiculoides
- Authors: Antunes, Ana Paula Martins
- Date: 2002
- Subjects: Azolla filiculoides Metal wastes -- Recycling Gold -- Recycling Platinum -- Recycling
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3894 , http://hdl.handle.net/10962/d1003726
- Description: Waste water from the mining industry is generally extremely complex and contains numerous species which influence the adsorption of the metals to any biomass. A variety of factors need to be addressed before treatment is considered viable. It is also beneficial to establish the binding characteristics of the metal of interest to maximise its interaction with the biomass to be utilised. Azalia filiculaides was investigated in the adsorption of gold(III), lead(II), iron(ID), copper(II) and platinum (IV). In batch studies, the optimum biomass and initial gold(III) concentrations were found to be 5 gIL and 8 mgIL respectively. The adsorption of gold(ID) is principally pH-dependent with optimal removal at pH 2. Lead(II), iron(III) and copper(II) did not compete with gold(III) adsorption under equimolar and simulated effluent conditions. Halides, with increasing affinity for gold (chloride < bromide < iodide), can affect gold uptake with the soft base, iodide, exhibiting the most inhibition (25%) and the hard base, chloride, O%. Mercaptoethanol (soft base) showed no interference in gold(III) adsorption while the presence of sulphate (hard base) and sulphite (borderline base) showed that concentrations in excess of 1 0 mM may adversely affect gold(ill) uptake, most likely due to competition for cationic sites on the biomass. Column studies, better suited to high volume treatment, indicated that a flow-rate of 5 mL/min and an initial gold(ill) concentration of 5 mgIL was optimal. Competitive effects between lead, iron, copper and gold again showed little or no interference. The halides, chloride, bromide and iodide, affect gold(ill) uptake similarly to the batch studies, while the bases mercaptoethanol and sulphate minimally affect gold(III) binding with sulphite severely hampering adsorption (70% inhibition). To optimise gold desorption, preliminary batch studies indicated that a ratio of 1:1 of adsorbentdesorbent was optimal, whilst gas purging of thiourea with oxygen, air and nitrogen decreased gold elution in proportion to decreased amounts of oxygen. A series of desorbents were utilised, in column studies, to optimise and determine the speciation of bound gold. The presence of an oxidant with thiourea enhanced desorption greater than 3 fold when compared with thiourea alone. Thiourea desorption studies, aided by the oxidant, suggest that gold is present in the + I and 0 oxidation states. Ultimately thiourea, perchloric acid and hydrochloric acid was found to be the most optimal elutant for gold (J 00% recovery). For selective metal recovery oflead and copper, pre-washing the plant material with water, utilising an acid (0.3 M nitric acid), pumping in an up-flow mode, and recycling the desorbent six times was found to be optimal elutant for gold (J 00% recovery). Cost analysis of utilising elutant versus incinerating the biomass for gold recovery indicated the latter as the most economical. Over a 5 cycle adsorption and desorption series, acid desorption before each adsorption cycle was found to result in greater than 92% desorption for lead and 96% for copper. Gold recovery was 97% with incineration. A preliminary study with gold effluent (Mine C) indicated that nickel and sulphate was removed in batch and column studies. Gold removal was found to be 100% and 4% in batch and column studies respectively. Adsorption of gold in the effluent study was accompanied by the release ofHt. Modifying the plant material with various reagents failed to identify the primary binding sites and the role of polysaccharides, proteins and lipids in gold(ill) uptake. The mode of gold binding is suggested as being initially ionic, this is very rapid, with the interaction of the anionic complex, [AuCI₄]". with the cationic biomass (PH 2). This eventually leads to the displacement of the chloride ligand(s) initiating covalent binding. Spectral studies of the chemical interaction between gold and the representative tannins indicated the protonated hydroxy groups to be responsible. All evidence suggests that the binding mechanisms of gold are not simple. Preliminary adsorption studies of platinum by Azalia filiculaides were conducted. Batch studies indicated that J gIL biomass concentration, initial platinum concentration of 20 mgIL and pH 2 are optimal, while the column studies indicated a flow-rate of! 0 rnL/min and initial platinum concentration of 20 mgIL as optimal. In the platinum effluent study, platinum showed a removal of 23 % and 2 J % for the batch and column studies respectively. Again adsorption was accompanied by //' release. Azalia filiculaides demonstrated its feasibility in the removal of gold and platinum from simulated as well as waste water solutions. Its potential viability as a biosorbent was demonstrated by the high recovery from synthetic solutions of greater than 99% for gold (2-10 mgIL), and greater than 89% for platinum (20 mgIL).
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- Date Issued: 2002
Reproductive conflicts in honeybee colonies
- Authors: Pirk, Christian Walter Werner
- Date: 2002
- Subjects: Honeybee -- Reproduction
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5755 , http://hdl.handle.net/10962/d1005443
- Description: In advanced eusocial hymenopteran societies workers have ovaries and can lay eggs, but are unable to mate. Workers are more related to their own offspring than to every other member of the colony. So worker reproduction contains both worker-worker and worker-queen conflict. The queen- worker conflict is discussed elsewhere, but if the queen mates with more than two males, worker policing should be selected to lower potential conflicts. However in the Cape honeybee it was predicted that worker policing is absent or less expressed than in other honeybee subspecies, because workers produce female offspring thelytokously. So laying workers and their offspring are nearly genetically identical, which results in the fact that other workers are as related to workers derived from eggs laid by the queen as laid by a worker. However, worker reproduction may be costly and therefore worker policing could be an evolutionary adaptation in the Cape honeybee to lower the costs derived from laying worker activity. Indeed, Cape honeybee colonies show efficient egg removal behaviour, suggesting that other factors like colony efficiency could favour egg removal behaviour. Since egg removal behaviour is a colony phenomenon, factors that affect colony performance could also affect egg removal behaviour. Egg removal behaviour was considerably affected by environmental changes, indicating that other tasks have a higher priority than egg removal behaviour. Thousands of queenright colonies of the neighbouring subspecies (A. m. scutellata) were taken over by laying A. m. capensis workers, showing that A. m. capensis workers are facultative social parasites. These observations strongly indicate that laying workers of A. m. capensis are able to evade worker policing and the inhibitory effects of the queen pheromones, but what potential strategies could these laying workers use to increase the survival of their eggs and evade the queen? On the one hand, egg removal behaviour is variable. One behavioural strategy of laying workers to achieve successful reproduction could be that they lay during periods with low egg removal behaviour. On the other hand, the inhibitory effect of the queen’s pheromones diminishes with distance. Maybe the level of egg removal also depends, like the inhibitory effect of the queen pheromones, on the distance from the queen. Indeed, further away from the queen the effect of the queen pheromone and the level of egg removal is reduced, making successful worker reproduction possible. In both subspecies, A. m. capensis and A. m. scutellata, egg removal behaviour is reduced further away from the queen. In the case of A. m. scutellata egg removal is lacking further away from the queen. This explains why colonies of scutellata are so prone to takeovers by laying Cape honeybee workers. One question in the context of parasitic Cape honeybees is how they manage to get into the host colony. One way could be that they get into the colonies during a natural colony merger which is common in African bees. Two unrelated colonies merged and it took them only 24 hours to show effective integration. Because both colonies are unrelated, the potential reproductive conflict among workers should be more strongly expressed than in a normal colony, which is not the result of a merger. Therefore, the effect of nestmate recognition for eggs on the egg removal behaviour was investigated. The results suggest that workers recognise the origin of an egg and that the standard policing experiments overestimate the level of egg removal and only represent relative values. Moreover, the results show that colony specific components on the eggs are more important than a postulated queen egg marking pheromone. Finally, for the first time empirical evidence from a population of the parasitic laying Cape honeybee workers, invading thousands of colonies of A. m. scutellata in northern South Africa, for a short-sighted selection theory is presented.
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- Date Issued: 2002
The biotransformation of phenolic pollutants using polyphenol oxidase
- Authors: Boshoff, Aileen
- Date: 2002
- Subjects: Polyphenol oxidase Sewage -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3976 , http://hdl.handle.net/10962/d1004035
- Description: The potential of using mushroom polyphenol oxidase (EC 1.14.18.1) as a biocatalyst for the biotransformation of phenols to produce catechols in an aqueous medium was investigated. Polyphenol oxidase is characterised by two distinct reactions i.e., the ortho-hydroxylation of phenols to catechols (cresolase activity) and the subsequent oxidation of catechols to orthoquinones (catecholase activity). In order to facilitate the development of a process to produce catechols, the accumulation of catechol as a true intermediate product released in the reaction system needed to be investigated, as its release had been disputed due to the oxidation of catechols to o-quinones. Using LC-MS, catechol products were successfully identified as true intermediate products formed during biocatalytic reactions in water.
- Full Text:
- Date Issued: 2002
The ecology of the red-billed quelea Quelea Quelea (Linnaeus) and other granivorous birds at Eastern Cape feedlots
- Authors: Whittington-Jones, Craig Alun
- Date: 2002
- Subjects: Quelea quelea -- South Africa -- Eastern Cape Granivores -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5618 , http://hdl.handle.net/10962/d1003286
- Description: Red-billed Quelea Quelea quelea have expanded their range in the Eastern Cape and now occur throughout the year in new areas. Recent changes in agricultural practice have probably been a contributing factor as flocks are larger than previously recorded and were more often associated with artificial food sources than natural grasses. Ringing and census data indicate that quelea have reduced movements during the non-breeding season and may display strong fidelity (over successive years) to dry season quarters with reliable food supplies. Although the recapture/recovery rate for quelea in the Eastern Cape (1.0-2.5%) was higher than the national average, it was still lower than expected and there may be considerable movement between nearby feeding sites. Some quelea breed locally, but most disperse during summer and their numbers at the feedlots were generally highest in winter and spring. The breeding season of quelea is later than other ploceids in the region and post-nuptial moult overlaps with winter. Replacement of primary wing feathers is relatively slow (124 days), and this is considered an adaptation to minimise disruption of flight capabilities and insulation. Significantly more quelea in the Eastern Cape have breeding plumage suffused with pink than in other southern African populations. However, during the non-breeding season there is apparently considerable intermixing between local populations and those from further north and the existence of a local sub-species is not supported. Seeds of two grass species, Echinochloa sp. and Urochloa panicoides, and two weed species, Amaranthus sp. and Chenopodium sp., were important in the diet of both quelea and Laughing Doves Streptopelia senegalensis. Maize comprised a large proportion of the diet of these species and losses at one ostrich feedlot were estimated at over R 17 000 in two years. Dependence on artificial food sources was generally greatest in winter and spring, but economically significant damage was not confined to this period. Alpha-chloralose showed good potential for reducing numbers of problem birds at livestock feedlots. However, the dynamic nature of problem bird populations favours a non-lethal management approach. Reduction of feed loss through manipulation of the ostrich ration could provide a relatively cheap and effective alternative to lethal control if applied appropriately.
- Full Text:
- Date Issued: 2002
The ecophysiology of selected coastal dune pioneer plants of the Eastern Cape
- Authors: Ripley, Bradford Sherman
- Date: 2002
- Subjects: Sand dune plants -- South Africa -- Eastern Cape Sand dune ecology -- South Africa -- Eastern Cape Sand dune plants -- Ecophysiology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4222 , http://hdl.handle.net/10962/d1003791
- Description: Understanding the mechanisms and adaptations that allow only certain species to thrive in the potentially stressful foredune environment requires a knowledge of the basic ecophysiology of foredune species. Ecophysiological measurements were conducted on the foredune pioneer species Arctotheca populifolia (Berg.) Norl., Ipomoea pes-caprae(L.) R. Br. and Scaevola plumieri (L.) Vahl. and showed significant differences among species with respect to the physiology associated with biomass production, water and nutrient relations. Differences related to CO₂ assimilation included differences in photosynthetic and respiratory rates, susceptibility to light stress and leaf and stem non-structural carbohydrate concentrations. These resulted in differences in primary production rates of shoots. Mechanisms leading to the differences in CO₂ assimilation among species included differences in stomatal behaviour, carboxylation efficiencies, efficiencies of utilisation of incident photosynthetic photon flux density (PPFD) and rates of ribulose-1,6-bisphosphate (RuBP) regeneration. Correlated with differences in photosynthetic capacity were differences in chlorophyll contents but not differences in leaf nitrogen content. Differences in interspecific stomatal behaviour resulted in significantly different transpiration rates which in combination with differences in assimilation rates resulted in differences in water-use efficiency. The absolute amounts of water transpired, although significantly different among species, were moderate to high in comparison with species from other ecosystems and were typical of mesophytes. Transpiration rates in combination with plant hydraulic conductances and soil water availability resulted in leaf water potentials that were not very negative and none of the investigated species showed evidence of osmotic adjustment. The volume of water transpired by each of the species per unit land surface area was estimated from the relationship between abiotic factors and plant water loss. These relationships varied among species and had varying degrees of predictability as a result of differences in stomatal behaviour between the three species. The water requirements of A. populifolia and S. plumieri were adequately met by the water supplied by rainfall and the water stored in the dune sands. It was therefore not necessary to invoke the utilisation of ground water or the process of internal dew formation to supply sufficient water to meet the requirements. However, I. pes-caprae despite its lower transpiration rates and due to its higher biomass, lost greater volumes of water per unit dune surface area than either A. populifolia or S. plumieri. This resulted in periods of potential water limitation for I. pes-caprae. Incident light was the most important determinant of leaf photosynthetic CO₂ assimilation and transpiration, particularly as a linear relationship between incident PPFD and atmospheric vapour pressure deficit (VPD) could be demonstrated. Whole plant photosynthetic production by S. plumieri was shown to be light limited as a result of mutual shading despite high incident and reflected PPFD occurring in the foredune environment. The leaf hair-layer of A. populifolia was shown to be important in reducing transmitted UV and hence reducing photoinhibition but it also caused reduced transpiration rates because of the thicker boundary layer and thus increased leaf temperatures. The nutrient content of above-ground plant parts of the investigated species were typical of higher plants despite the low nutrient content measured for the dune soils. With the possible exception of nitrogen the nutrient demand created by above-ground production was adequately met by the supply of nutrients either from sand-water or from aquifer-water transpired by the plants. Differences in the volumes of water transpired, and hence the quantity of nutrients potentially taken up via the transpiration stream, resulted in interspecific differences in above-ground plant macronutrient content. The reallocation patterns of nutrients differed both between the various nutrients measured and interspecifically. Standing biomass and the density of plants per unit land area was low in comparison to that of other ecosystems and was different among investigated species. This may be important in maintaining the adequate supply of resources (water, nutrients and light). As a result of the interspecific differences in biomass when production was expressed per unit land surface area the resultant productivity was not dissimilar among species. Productivity was high when comparisons were made with species from other ecosystems. No single resource (water, nutrients or light) could be identified as the controlling factor in the foredune environment and a combination of both resource stress and environmental disturbance are likely to be involved. Physiology, production, growth and growth characteristics conveyed certain adaptive advantages to these species in respect to both resource stress and environmental disturbance. Interspecific differences in these adaptations can be used to offer explanations for the observed microhabitat preferences of the three investigated species. Furthermore features common to all three species offer some explanations as to why these species and not others are able to inhabit the foredunes.
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- Date Issued: 2002
The growth and reproduction of the freshwater limpet Burnupia stenochorias (Pulmonata, Ancylidae), and an evaluation of its use as an ecotoxicology indicator in whole effluent testing
- Authors: Davies-Coleman, H D (Heather D)
- Date: 2002
- Subjects: Ancylidae Effluent quality Toxicity testing
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5703 , http://hdl.handle.net/10962/d1005389
- Description: For the protection of the ecological Reserve in South Africa, the proposed introduction of compulsory toxicity testing in the licensing of effluent discharges necessitates the development of whole effluent toxicity testing. The elucidation of the effects of effluent on the local indigenous populations of organisms is essential before hazard and risk assessment can be undertaken. The limpet Burnupia stenochorias, prevalent in the Eastern Cape of South Africa, was chosen to represent the freshwater molluscs as a potential toxicity indicator. Using potassium dichromate (as a reference toxicant) and a textile whole effluent, the suitability of B. stenochorias was assessed under both acute and chronic toxicity conditions in the laboratory. In support of the toxicity studies, aspects of the biology of B. stenochorias were investigated under both natural and laboratory conditions. Using Principal Component and Discriminant Function Analyses, the relative shell morphometrics of three feral populations of B. stenochorias were found to vary. Length was shown to adequately represent growth of the shell, although the inclusion of width measurements is more statistically preferable. Two of the feral populations, one in impacted water, were studied weekly for 52 weeks to assess natural population dynamics. Based on the Von Bertalanffy Growth Equation, estimates of growth and longevity were made for this species, with growth highly seasonal. Age is not easily discerned from shell size. Egg laying occurred all year round, with early summer (peak egg lay), mid summer (a second, smaller peak in egg lay), and winter (limited presence of eggs) phases. In toxicity testing, consideration is given to the choice of the test organism based on age and sexual development. Consequently, the sexual development of B. stenochorias relative to shell length was determined with the aid of histological examinations of transverse sections of limpets, of all sizes, collected over one year. Limpets less than 3mm shell length were found to be immature in the development of the oocytes and spermatozoa, and were later chosen for acute toxicity tests. A laboratory diet was developed, for both culturing and maintaining of the limpets during toxicity tests; however, the diet requires optimisation. Under laboratory conditions, growth was linear, and individual fecundity highly variable. Successful methods for the collection of limpets from naturally occurring populations, and their acclimation to the laboratory were developed. Three B. stenochorias populations, representing different hydrological and water quality conditions, were compared to a laboratory population (maintained for three years) in their responses to the textile whole effluent and potassium dichromate. Under acute conditions, variability of mortality between limpet populations and between seasons was consistent with acceptable international standards. However, seasonal differences between feral limpets were apparent, with early summer limpets significantly more susceptible to both potassium dichromate and textile effluent than winter limpets. Although mortality occurred within the effluent at all concentrations, no 96 hour LC₅₀ values were obtained. The chronic toxicity effects of the textile whole effluent were assessed over the entire life cycle of B. stenochorias, based on survival, growth and reproductive effects. Lower concentrations of effluent (# 10%) gave greater variability of responses and toxicity than higher concentrations, with a 43 day LC₅₀ of 3.9% effluent. The No Observed Effect Concentrations for survival (over 43 days) were calculated in consecutive years as 0.1% and 1% effluent. Survival is considered a useful tool for determining toxicity endpoints using B. stenochorias. Limpet growth remained linear in effluent, with an apparent stimulation of growth at the 3-10% effluent concentration, confusing the toxicity and variability assessments. The possible addition of nutrients from the effluent points to either a potential inadequacy of the food quality provided in the chronic assessment, or the presence in the effluent of growth stimulants. Growth was also found to be too variable to allow adequate statistical conclusions about the toxicity of the effluent, although it is suggested that growth may be useful in the assessment of single compounds. Despite large individual variability in fecundity, statistical differences were discernible between effluent concentrations. The application of fecundity of B. stenochorias in hazard assessment therefore warrants further investigation. It was concluded that an assessment of textile whole effluent toxicity to B. stenochorias over an entire life cycle, and an F1 generation, is unnecessary. The development of the bucket/plastic bag method for both acute and chronic toxicity assessment of B. stenochorias was useful. In the final assessment of the usefulness of B. stenochorias as a toxicity indicator, toxicity endpoints were compared with those of the standard laboratory organism Daphnia pulex. Both in acute and chronic toxicity, B. stenochorias was found to be more sensitive. B. stenochorias is therefore considered valuable as a South African freshwater molluscan ecotoxicological indicator, with a place in hazard assessment, although further development and research is necessary before the limpet can be effectively used.
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- Date Issued: 2002
The life history of selected coastal foredune species of South Africa
- Authors: Knevel, Irma Cornelia
- Date: 2002
- Subjects: Sand dune plants -- South Africa Sand dune ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4207 , http://hdl.handle.net/10962/d1003776
- Description: South African dune fields are severely threatened by human expansion and in the long run the stabilisation of many dunes will be necessary. The alien grass Ammophila arenaria is the most important drift sand stabiliser at present in South Africa. Although not invasive, the current impact of A. arenaria on the dune systems of South Africa is considerable, and thus the stabilising benefit of the grass seemed to may be outweighed by its negative consequences. It is therefore preferable to use indigenous sand stabilising species. In order to define guidelines for the application of indigenous plants for stabilisation, their autecology should be studied first to enhance the chance of successful stabilisation results. The main aim of the present thesis was to gather information on the life history processes of selected indigenous, sand stabilising foredune species. To investigate the growth of foredune pioneer species, the common pioneer Scaevola plumieri was followed over a three-year period to determine the growth season and leaf phenology. Soil-borne pathogens are known to influence the growth and vegetation dynamics of foredune species. To examine this effect on the South African foredunes the rhizosphere soil and the roots of several species were studied. To test the effect of the nematode fauna on succeeding plant species a transplantation experiment was carried out. The seed stage is the only life-cycle stage that can survive unfavourable conditions. Therefore, the seed ecology of several foredune species was studied extensively to determine the reproductive season, the seed production, the fate of seeds after shedding (germination, seawater dispersal), germination requirements and seed bank strategy. Seeds of the species Arctotheca populifolia, Ipomoea pes-caprae, Myrica cordifolia, and Scaevola plumieri were subjected to germination trials, field observations on seedling survival, and scarification and stratification experiments. This was done to obtain information about the germination requirements and to determine the reproductive season and growth season. The seed bank strategy of the foredune species, as well as the seed bank density, was determined by extensive sampling along the Cape coast. The species S. plumieri thrived under sand accretion situations, which makes it a good candidate for stabilisation purposes. The growth of S. plumieri was seasonal, with the highest leaf production during spring and summer. The stem position on the foredune had a strong effect on the overall performance of S. plumieri , with the stems situated on the landward face of the foredune showing higher leaf and seed production. Theiii nematode survey of soil and roots of several foredune species showed that all plant species featured a specific nematode fauna in the rhizosphere soil and the roots. The specific nematode fauna affected the growth of foreign plant species in the transplantation experiment, resulting in a lower root and/or shoot biomass production. Most of the foredune species produce seeds from spring to late summer. For S. plumieri the position of the stem on the dunes, as well as the predation of unripe seeds affected the number of seeds produced. The highest production was found for the landward faced stems. The S. plumieri seeds were able to float on seawater for at least three months without losing viability, as was observed for seeds of I. pes-caprae. The seeds of M. cordifolia, however, sank after a few days, but their viability was not affected. The rhizome fragments of A. arenaria and S. virginicus floated for 120 days, whereas the fragments of E. villosa sunk after one day. The viability of S. virginicus fragments was affected by the duration in seawater by an increase in sprouting time. The seeds of all species tested germinated readily under controlled conditions, except S. plumieri seeds which required a long lag-phase before germination. In the field the seeds of A. populifolia, I. pes-caprae and S. plumieri germinated, producing many seedlings. Only the seedlings of A. populifolia and S. plumieri survived. Of the species found in the foredunes 57% was represented in the soil seed bank. For most species, the seeds that were found in the seed bank showed viability of at least 40%. Many of the seeds found were older than one year, suggesting a short-term persistent seed bank. The present study is a start in filling the gap in information on dune pioneer and foredune species. The conclusion was that in general all species in the present study were easy to grow under controlled conditions, and thus could be used for stabilisation purposes. When the more rapidly growing pioneer species are planted in combination with succeeding foredune species, a functional and aesthetic ecosystem could be created.
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- Date Issued: 2002
The medicinal chemistry of the isomers of the cyclic dipeptide: cyclo(Trp-Pro)
- Authors: Jamie, Hajierah
- Date: 2002
- Subjects: Pharmaceutical chemistry , Cyclic compounds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:11023 , http://hdl.handle.net/10948/281 , Pharmaceutical chemistry , Cyclic compounds
- Description: The isomers of cyclo(Trp-Pro) (cyclo(L-Trp-L-Pro), cyclo(L-Trp-D-Pro), cyclo(D-Trp-LPro) and cyclo(D-Trp-D-Pro)) have been successfully synthesized and screened for biological activity. High percentage yields were obtained by using the three phase synthesis system, which involves the synthesis of the intermediate protected linear dipeptides, followed by the removal of the protecting Boc groups. This step is followed by cyclization and crystallization of the isomers. The diketopiperazines rings of cyclo(L-Trp-L-Pro) and cyclo(D-Trp-D-Pro) contain cisamide bonds, while cyclo(L-Trp-D-Pro) and cyclo(D-Trp-L-Pro) contain trans-amide bonds. These bonds govern the conformation of the diketopiperazines ring. The isomers have shown different degrees of biological activity, possibly as a result of the orientation of the side chain of tryptophan and this difference in conformation, leading to varying interactions between isomer and a range of receptors. Under experimental conditions, 10-3 M cyclo(L-Trp-D-Pro) and cyclo(D-Trp-L-Pro) showed effective anticancer activity against the cervical cancer cell line, HeLa, resulting in a <50% reduction in cell viability. Cytotoxicity screening with cyclo(D-Trp-L-Pro) indicated that it was hepatocyte-specific in its toxicity, whilst the other isomers were cytotoxic against the other cell types tested. At 1mg/ml, cyclo(L-Trp-L-Pro) proved to be an effective antimicrobial agent against Gram positive bacteria, while cyclo(L-Trp-DPro) effectively inhibited the growth of the Gram negative bacteria, Esherichia coli. Cyclo(D-Trp-L-Pro) proved to be effective against Streptococcus, while cyclo(D-Trp-DPro) effectively reduced viability of the yeast, Candida albicans. Cyclo(D-Trp-L-Pro) was the only isomer to show Ca2+-channel antagonism, whilst the other isomers resulted in opening of the Ca2+-channel. No effects were observed on K+-channel activity for all the isomers tested. The isomers also proved to be valuable antiarrhythmic agents by effectively reducing the time spent in ventricular tachycardia and arrhythmia, as well as decreasing the time for the heart rate to return to a normal sinus rhythm. Furthermore, cyclo(L-Trp-D-Pro) showed positive chronotropic activity, while cyclo(D-Trp-L-Pro) ii showed negative chronotropic activity. In addition, cyclo(L-Trp-D-Pro) and cyclo(D-Trp- L-Pro) also increased the coronary flow rate. 0.125 1 mM Cyclo(L-Trp-D-Pro) decreased aggregation in washed platelets induced by thrombin. All isomers increased adhesion to an artificial surface when the platelets were stimulated by ADP, yet caused reduced adhesion when the platelets were stimulated by thrombin. These results prove the potential of these compounds as novel agents in a range of biological fields, indicating that a combination of L- and D- amino acids may prove more effective than an agent consisting solely of L-amino acids.
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- Date Issued: 2002
The political ecology of wildlife conservation in Kenya, 1895-1975
- Authors: Matheka, Reuben M
- Date: 2002
- Subjects: Wildlife conservation -- Kenya Environmental policy -- Kenya Wildlife conservation -- Political aspects -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2595 , http://hdl.handle.net/10962/d1007530
- Description: The study examines the development of wildlife conservation policy and practice in Kenya from 1895 to 1975. Started by the colonial state as part of its resource control programme, wildlife conservation in Kenya gradually became an important aspect of the country's economy chiefly because of its significance as the basis of a vibrant tourist industry. The conservation programme was also important to conservationists who viewed Kenya's wildlife as a heritage to humanity. Similarly, local communities, which were affected in various ways by wildlife conservation policies, had their own perceptions of the programme. All this led to the proliferation of groups whose interests were potentially conflicting. Wildlife conservation in Kenya during the period under examination was thus characterised by various struggles between interest groups such as conservationists, the state, and local communities. The struggles centred around such issues as the costs and benefits of conservation and were manifested through anti-conservation activities like the poaching of wild animals by dissatisfied groups. These struggles changed over time in line with social, economic, political, and ecological developments. International events/processes (such as the two world wars, economic booms/depressions, and decolonisation) triggered local processes which influenced conservationism either positively or negatively. Wildlife conservation in Kenya during the period under study was dynamic. The thesis challenges the myth of a monolithic 'colonial' wildlife policy often implied in many studies on the subject. The thesis also lays emphasis on the ecological basis of wildlife conservation while recognising the impact of social, political, and economic developments on the evolution of wildlife conservation policy and practice in Kenya. The country's 'geography' not only provided the foundation for conservation but also influenced the programme over time. Droughts, floods, army worm infestations, and other 'natural' occurrences interacted with social and economic changes, such as population growth and the development of capitalism, to shape conservation policy. The conservation programme was thus influenced by a complex interaction of a variety of factors.
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- Date Issued: 2002