Assessing the use of international business strategies among automotive wiring harness manufacturers in the Nelson Mandela Metropole
- Authors: Mears, Michael
- Date: 2007
- Subjects: International trade , International business enterprises -- South Africa , Corporations, Foreign , International economic relations
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8741 , http://hdl.handle.net/10948/764 , International trade , International business enterprises -- South Africa , Corporations, Foreign , International economic relations
- Description: Since 1994, the opening up of the South African economy has presented South African companies with opportunities to exploit the bigger global market and also with challenges of competing with international companies. Companies must consider both external environmental forces and internal organizational factors before arriving at a suitable international strategy. This treatise explores the wiring harness industry in the Nelson Mandela Bay to determine whether the industry is adopting international strategies in line with globalization, thus ensuring sustained growth and profitability. A literature survey was conducted to discover the main strategies that are used by companies in order to achieve global competitiveness. These strategies were used in conjunction with Porter's (1990) theory of National Competitive Advantage to analyse the wiring harness industry in the Nelson Mandela Bay. Porter's (1990) theory of National Competitive Advantage was used to analyse the competitiveness of the wiring harness industry in the Nelson Mandela Bay. A questionnaire was developed to test the degree to which the wiring harness industry in the Nelson Mandela Bay is in agreement with the findings of the literature study. This information was used to determine whether the wiring harness industry in the Nelson Mandela Bay is following global trends to remain profitable.
- Full Text:
- Date Issued: 2007
- Authors: Mears, Michael
- Date: 2007
- Subjects: International trade , International business enterprises -- South Africa , Corporations, Foreign , International economic relations
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8741 , http://hdl.handle.net/10948/764 , International trade , International business enterprises -- South Africa , Corporations, Foreign , International economic relations
- Description: Since 1994, the opening up of the South African economy has presented South African companies with opportunities to exploit the bigger global market and also with challenges of competing with international companies. Companies must consider both external environmental forces and internal organizational factors before arriving at a suitable international strategy. This treatise explores the wiring harness industry in the Nelson Mandela Bay to determine whether the industry is adopting international strategies in line with globalization, thus ensuring sustained growth and profitability. A literature survey was conducted to discover the main strategies that are used by companies in order to achieve global competitiveness. These strategies were used in conjunction with Porter's (1990) theory of National Competitive Advantage to analyse the wiring harness industry in the Nelson Mandela Bay. Porter's (1990) theory of National Competitive Advantage was used to analyse the competitiveness of the wiring harness industry in the Nelson Mandela Bay. A questionnaire was developed to test the degree to which the wiring harness industry in the Nelson Mandela Bay is in agreement with the findings of the literature study. This information was used to determine whether the wiring harness industry in the Nelson Mandela Bay is following global trends to remain profitable.
- Full Text:
- Date Issued: 2007
Assessment of antibacterial potentials of Garcinia Kola seed extracts and their interactions with antibiotics
- Authors: Sibanda, Thulani
- Date: 2007
- Subjects: Drug resistance in microorganisms , Garcinia , Antibiotics , Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11242 , http://hdl.handle.net/10353/71 , Drug resistance in microorganisms , Garcinia , Antibiotics , Medicinal plants
- Description: The antibacterial potency of the extracts of the seed of Garcinia kola (bitter kola) was investigated in this study against a panel of referenced, environmental and clinical bacterial strains. The killing rates of the active extract as well as their potential for combination antibacterial therapy with standard antibiotics were also elucidated using standard procedures. The aqueous and acetone extracts of the seed were screened for activity against 27 bacterial isolates. The aqueous extract exhibited activity mainly against Gram positive organisms with Minimum inhibitory concentration (MIC) values ranging from 5 mgml-1 – 20 mgml-1, while the acetone extract showed activity against both Gram negative and Gram positive organisms with MIC values ranging from 10 mgml-1 - 0.156 mgml-1. The acetone extract also showed rapid bactericidal activity against Staphylococcus aureus ATCC 6538 with a 3.097 Log10 reduction in counts within 4 hours at 0.3125 mgml-1 and a 1.582 Log10 reduction against Proteus vulgaris CSIR 0030 at 5 mgml-1 after 1 hour. In addition, the aqueous, methanol and acetone extracts of the seeds also exhibited activity against four clinical strains of Staphylococcus isolated from wound sepsis specimens. The MIC values for the aqueous extract were 10 mgml-1 for all the isolates while the acetone and methanol extracts had lower values ranging from 0.3125 - 0.625 mgml-1. The acetone extract was strongly bactericidal against Staphylococcus aureus OKOH3 resulting in a 2.70 Log10 reduction in counts at 1.25 mgml-1 within 4 hours of exposure and a complete elimination of the organism after 8 hours. The bactericidal vi activity of the same extract against Staphylococcus aureus OKOH1 was weak, achieving only a 2.92 Log10 reduction in counts at 1.25 mgml-1 (4× MIC) in 24 hours. In the test for interactions between the acetone extract of the seeds and antibiotics, synergistic interactions were observed largely against Gram positive organisms using the FIC indices, (indices of 0.52 - 0.875) with combinations against Gram negatives yielding largely antagonistic interactions (indices of 2.0 to 5.0). Synergy (≥ 1000 times or ≥ 3 Log10 potentiation of the bactericidal activity) against both Gram negative and Gram positive organisms was detected by time kill assays mainly involving the antibiotics tetracycline, chloramphenicol, amoxycillin and penicillin G. Combinations involving erythromycin and ciprofloxacin consistently gave antagonistic or indifferent interactions. We conclude that the acetone extract of Garcinia kola seeds possess strong bactericidal activities against both Gram positive and Gram negative organisms and can be therapeutically useful in the treatment of bacterial infections including the problematic staphylococcal wound infections. In addition, the acetone extract can be a potential source of broad spectrum resistance modifying compounds that can potentially improve the performance of antibiotics in the treatment of drug resistant infections.
- Full Text:
- Date Issued: 2007
- Authors: Sibanda, Thulani
- Date: 2007
- Subjects: Drug resistance in microorganisms , Garcinia , Antibiotics , Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11242 , http://hdl.handle.net/10353/71 , Drug resistance in microorganisms , Garcinia , Antibiotics , Medicinal plants
- Description: The antibacterial potency of the extracts of the seed of Garcinia kola (bitter kola) was investigated in this study against a panel of referenced, environmental and clinical bacterial strains. The killing rates of the active extract as well as their potential for combination antibacterial therapy with standard antibiotics were also elucidated using standard procedures. The aqueous and acetone extracts of the seed were screened for activity against 27 bacterial isolates. The aqueous extract exhibited activity mainly against Gram positive organisms with Minimum inhibitory concentration (MIC) values ranging from 5 mgml-1 – 20 mgml-1, while the acetone extract showed activity against both Gram negative and Gram positive organisms with MIC values ranging from 10 mgml-1 - 0.156 mgml-1. The acetone extract also showed rapid bactericidal activity against Staphylococcus aureus ATCC 6538 with a 3.097 Log10 reduction in counts within 4 hours at 0.3125 mgml-1 and a 1.582 Log10 reduction against Proteus vulgaris CSIR 0030 at 5 mgml-1 after 1 hour. In addition, the aqueous, methanol and acetone extracts of the seeds also exhibited activity against four clinical strains of Staphylococcus isolated from wound sepsis specimens. The MIC values for the aqueous extract were 10 mgml-1 for all the isolates while the acetone and methanol extracts had lower values ranging from 0.3125 - 0.625 mgml-1. The acetone extract was strongly bactericidal against Staphylococcus aureus OKOH3 resulting in a 2.70 Log10 reduction in counts at 1.25 mgml-1 within 4 hours of exposure and a complete elimination of the organism after 8 hours. The bactericidal vi activity of the same extract against Staphylococcus aureus OKOH1 was weak, achieving only a 2.92 Log10 reduction in counts at 1.25 mgml-1 (4× MIC) in 24 hours. In the test for interactions between the acetone extract of the seeds and antibiotics, synergistic interactions were observed largely against Gram positive organisms using the FIC indices, (indices of 0.52 - 0.875) with combinations against Gram negatives yielding largely antagonistic interactions (indices of 2.0 to 5.0). Synergy (≥ 1000 times or ≥ 3 Log10 potentiation of the bactericidal activity) against both Gram negative and Gram positive organisms was detected by time kill assays mainly involving the antibiotics tetracycline, chloramphenicol, amoxycillin and penicillin G. Combinations involving erythromycin and ciprofloxacin consistently gave antagonistic or indifferent interactions. We conclude that the acetone extract of Garcinia kola seeds possess strong bactericidal activities against both Gram positive and Gram negative organisms and can be therapeutically useful in the treatment of bacterial infections including the problematic staphylococcal wound infections. In addition, the acetone extract can be a potential source of broad spectrum resistance modifying compounds that can potentially improve the performance of antibiotics in the treatment of drug resistant infections.
- Full Text:
- Date Issued: 2007
Assessment of antibacterial potentials of Garcinia Kola seed extracts and their interactions with antibiotics
- Authors: Sibanda, Thulani
- Date: 2007
- Subjects: Drug resistance in microorganisms , Garcinia , Antibiotics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19236 , vital:43038
- Description: The antibacterial potency of the extracts of the seed of Garcinia kola (bitter kola) was investigated in this study against a panel of referenced, environmental and clinical bacterial strains. The killing rates of the active extract as well as their potential for combination antibacterial therapy with standard antibiotics were also elucidated using standard procedures. The aqueous and acetone extracts of the seed were screened for activity against 27 bacterial isolates. The aqueous extract exhibited activity mainly against Gram positive organisms with Minimum inhibitory concentration (MIC) values ranging from 5 mgml-1 – 20 mgml-1, while the acetone extract showed activity against both Gram negative and Gram positive organisms with MIC values ranging from 10 mgml-1 - 0.156 mgml-1. The acetone extract also showed rapid bactericidal activity against Staphylococcus aureus ATCC 6538 with a 3.097 Log10 reduction in counts within 4 hours at 0.3125 mgml-1 and a 1.582 Log10 reduction against Proteus vulgaris CSIR 0030 at 5 mgml-1 after 1 hour. In addition, the aqueous, methanol and acetone extracts of the seeds also exhibited activity against four clinical strains of Staphylococcus isolated from wound sepsis specimens. The MIC values for the aqueous extract were 10 mgml-1 for all the isolates while the acetone and methanol extracts had lower values ranging from 0.3125 - 0.625 mgml-1. The acetone extract was strongly bactericidal against Staphylococcus aureus OKOH3 resulting in a 2.70 Log10 reduction in counts at 1.25 mgml-1 within 4 hours of exposure and a complete elimination of the organism after 8 hours. The bactericidal activity of the same extract against Staphylococcus aureus OKOH1 was weak, achieving only a 2.92 Log10 reduction in counts at 1.25 mgml-1 (4× MIC) in 24 hours. In the test for interactions between the acetone extract of the seeds and antibiotics, synergistic interactions were observed largely against Gram positive organisms using the FIC indices, (indices of 0.52 - 0.875) with combinations against Gram negatives yielding largely antagonistic interactions (indices of 2.0 to 5.0). Synergy (≥ 1000 times or ≥ 3 Log10 potentiation of the bactericidal activity) against both Gram negative and Gram positive organisms was detected by time kill assays mainly involving the antibiotics tetracycline, chloramphenicol, amoxycillin and penicillin G. Combinations involving erythromycin and ciprofloxacin consistently gave antagonistic or indifferent interactions. We conclude that the acetone extract of Garcinia kola seeds possess strong bactericidal activities against both Gram positive and Gram negative organisms and can be therapeutically useful in the treatment of bacterial infections including the problematic staphylococcal wound infections. In addition, the acetone extract can be a potential source of broad spectrum resistance modifying compounds that can potentially improve the performance of antibiotics in the treatment of drug resistant infections. , Thesis (MSc)-- Microbiology, University of Fort Hare, 2007
- Full Text:
- Date Issued: 2007
- Authors: Sibanda, Thulani
- Date: 2007
- Subjects: Drug resistance in microorganisms , Garcinia , Antibiotics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19236 , vital:43038
- Description: The antibacterial potency of the extracts of the seed of Garcinia kola (bitter kola) was investigated in this study against a panel of referenced, environmental and clinical bacterial strains. The killing rates of the active extract as well as their potential for combination antibacterial therapy with standard antibiotics were also elucidated using standard procedures. The aqueous and acetone extracts of the seed were screened for activity against 27 bacterial isolates. The aqueous extract exhibited activity mainly against Gram positive organisms with Minimum inhibitory concentration (MIC) values ranging from 5 mgml-1 – 20 mgml-1, while the acetone extract showed activity against both Gram negative and Gram positive organisms with MIC values ranging from 10 mgml-1 - 0.156 mgml-1. The acetone extract also showed rapid bactericidal activity against Staphylococcus aureus ATCC 6538 with a 3.097 Log10 reduction in counts within 4 hours at 0.3125 mgml-1 and a 1.582 Log10 reduction against Proteus vulgaris CSIR 0030 at 5 mgml-1 after 1 hour. In addition, the aqueous, methanol and acetone extracts of the seeds also exhibited activity against four clinical strains of Staphylococcus isolated from wound sepsis specimens. The MIC values for the aqueous extract were 10 mgml-1 for all the isolates while the acetone and methanol extracts had lower values ranging from 0.3125 - 0.625 mgml-1. The acetone extract was strongly bactericidal against Staphylococcus aureus OKOH3 resulting in a 2.70 Log10 reduction in counts at 1.25 mgml-1 within 4 hours of exposure and a complete elimination of the organism after 8 hours. The bactericidal activity of the same extract against Staphylococcus aureus OKOH1 was weak, achieving only a 2.92 Log10 reduction in counts at 1.25 mgml-1 (4× MIC) in 24 hours. In the test for interactions between the acetone extract of the seeds and antibiotics, synergistic interactions were observed largely against Gram positive organisms using the FIC indices, (indices of 0.52 - 0.875) with combinations against Gram negatives yielding largely antagonistic interactions (indices of 2.0 to 5.0). Synergy (≥ 1000 times or ≥ 3 Log10 potentiation of the bactericidal activity) against both Gram negative and Gram positive organisms was detected by time kill assays mainly involving the antibiotics tetracycline, chloramphenicol, amoxycillin and penicillin G. Combinations involving erythromycin and ciprofloxacin consistently gave antagonistic or indifferent interactions. We conclude that the acetone extract of Garcinia kola seeds possess strong bactericidal activities against both Gram positive and Gram negative organisms and can be therapeutically useful in the treatment of bacterial infections including the problematic staphylococcal wound infections. In addition, the acetone extract can be a potential source of broad spectrum resistance modifying compounds that can potentially improve the performance of antibiotics in the treatment of drug resistant infections. , Thesis (MSc)-- Microbiology, University of Fort Hare, 2007
- Full Text:
- Date Issued: 2007
Assessment of spatial variation in carbon utilization by benthic and pelagic invertebrates in a temperate South African estuary using stable isotope signatures
- Richoux, Nicole B, Froneman, P William
- Authors: Richoux, Nicole B , Froneman, P William
- Date: 2007
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/457923 , vital:75695 , xlink:href="https://doi.org/10.1016/j.ecss.2006.09.007 "
- Description: Stable isotope analyses (δ13C and δ15N) were used to evaluate the spatial variations in carbon flow from primary producers to consumers at two sites in the temperate and permanently open Kariega Estuary on the southeastern coast of South Africa during October 2005 and February 2006. One site was located opposite a salt marsh while the second was upstream of the marsh. Except for significantly enriched δ13C values of Zostera capensis and surface sediments near the salt marsh, the δ13C and δ15N signatures of the producers were similar between sites. The invertebrates were clustered into groups roughly corresponding to the predominant feeding modes. The suspension feeders showed δ13C values closest to the seston, whereas the deposit feeders, detritivores and scavengers/predators had more enriched δ13C values reflecting primary carbon sources that were likely a combination of seston, Spartina maritima and Z. capensis at the upstream site, with an increased influence of benthic algae and Z. capensis at the salt marsh site. The δ15N signatures of the consumers showed a stepwise continuum rather than distinct levels of fractionation, indicating highly complex trophic linkages and significant dietary overlap among the species. Consumers exhibited significantly enriched δ13C values at the salt marsh site, an effect that was attributed to enriched Z. capensis detritus in this region in addition to increased phytoplankton biomass in their diets compared with invertebrates living upstream. The data reinforce the concept that between-site variations in the stable isotope ratios of consumers can result not only from dietary shifts, but also from alterations in the isotope ratios of primary producers.
- Full Text:
- Date Issued: 2007
- Authors: Richoux, Nicole B , Froneman, P William
- Date: 2007
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/457923 , vital:75695 , xlink:href="https://doi.org/10.1016/j.ecss.2006.09.007 "
- Description: Stable isotope analyses (δ13C and δ15N) were used to evaluate the spatial variations in carbon flow from primary producers to consumers at two sites in the temperate and permanently open Kariega Estuary on the southeastern coast of South Africa during October 2005 and February 2006. One site was located opposite a salt marsh while the second was upstream of the marsh. Except for significantly enriched δ13C values of Zostera capensis and surface sediments near the salt marsh, the δ13C and δ15N signatures of the producers were similar between sites. The invertebrates were clustered into groups roughly corresponding to the predominant feeding modes. The suspension feeders showed δ13C values closest to the seston, whereas the deposit feeders, detritivores and scavengers/predators had more enriched δ13C values reflecting primary carbon sources that were likely a combination of seston, Spartina maritima and Z. capensis at the upstream site, with an increased influence of benthic algae and Z. capensis at the salt marsh site. The δ15N signatures of the consumers showed a stepwise continuum rather than distinct levels of fractionation, indicating highly complex trophic linkages and significant dietary overlap among the species. Consumers exhibited significantly enriched δ13C values at the salt marsh site, an effect that was attributed to enriched Z. capensis detritus in this region in addition to increased phytoplankton biomass in their diets compared with invertebrates living upstream. The data reinforce the concept that between-site variations in the stable isotope ratios of consumers can result not only from dietary shifts, but also from alterations in the isotope ratios of primary producers.
- Full Text:
- Date Issued: 2007
Automated estimation and analyses of meteorological drought characteristics from monthly rainfall data
- Smakhtin, V U, Hughes, Denis A
- Authors: Smakhtin, V U , Hughes, Denis A
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:7078 , http://hdl.handle.net/10962/d1009746
- Description: The paper describes a new software package for automated estimation, display and analyses of various drought indices – continuous functions of precipitation that allow quantitative assessment of meteorological drought events to be made. The software at present allows up to five different drought indices to be estimated. They include the Decile Index (DI), the Effective Drought Index (EDI), the Standardized Precipitation Index (SPI) and deviations from the long-term mean and median value. Each index can be estimated from point and spatially averaged rainfall data and a number of options are provided for months' selection and the type of the analysis, including a running mean, single value or multiple annual values. The software also allows spell/run analysis to be performed and maps of a specific index to be constructed. The software forms part of the comprehensive computer package, developed earlier and designed to perform the multitude of water resources analyses and hydro-meteorological data processing. The 7-step procedure of setting up and running a typical drought assessment application is described in detail. The examples of applications are given primarily in the specific context of South Asia where the software has been used.
- Full Text:
- Date Issued: 2007
- Authors: Smakhtin, V U , Hughes, Denis A
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:7078 , http://hdl.handle.net/10962/d1009746
- Description: The paper describes a new software package for automated estimation, display and analyses of various drought indices – continuous functions of precipitation that allow quantitative assessment of meteorological drought events to be made. The software at present allows up to five different drought indices to be estimated. They include the Decile Index (DI), the Effective Drought Index (EDI), the Standardized Precipitation Index (SPI) and deviations from the long-term mean and median value. Each index can be estimated from point and spatially averaged rainfall data and a number of options are provided for months' selection and the type of the analysis, including a running mean, single value or multiple annual values. The software also allows spell/run analysis to be performed and maps of a specific index to be constructed. The software forms part of the comprehensive computer package, developed earlier and designed to perform the multitude of water resources analyses and hydro-meteorological data processing. The 7-step procedure of setting up and running a typical drought assessment application is described in detail. The examples of applications are given primarily in the specific context of South Asia where the software has been used.
- Full Text:
- Date Issued: 2007
Bee-hawking by the wasp, Vespa velutina, on the honeybees Apis cerana and A. mellifera
- Tan, K, Radloff, Sarah E, Li, J J, Hepburn, H Randall, Yang, Ming-Xian, Zhang, L J, Neumann, Peter
- Authors: Tan, K , Radloff, Sarah E , Li, J J , Hepburn, H Randall , Yang, Ming-Xian , Zhang, L J , Neumann, Peter
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:6941 , http://hdl.handle.net/10962/d1011965
- Description: The vespine wasps, Vespa velutina, specialise in hawking honeybee foragers returning to their nests. We studied their behaviour in China using native Apis cerana and introduced A. mellifera colonies. When the wasps are hawking, A. cerana recruits threefold more guard bees to stave off predation than A. mellifera. The former also utilises wing shimmering as a visual pattern disruption mechanism, which is not shown by A. mellifera. A. cerana foragers halve the time of normal flight needed to dart into the nest entrance, while A. mellifera actually slows down in sashaying flight manoeuvres. V. velutina preferentially hawks A. mellifera foragers when both A. mellifera and A. cerana occur in the same apiary. The pace of wasp-hawking was highest in mid-summer but the frequency of hawking wasps was three times higher at A. mellifera colonies than at the A. cerana colonies. The wasps were taking A. mellifera foragers at a frequency eightfold greater than A. cerana foragers. The final hawking success rates of the wasps were about three times higher for A. mellifera foragers than for A. cerana. The relative success of native A. cerana over European A. mellifera in thwarting predation by the wasp V. velutina is interpreted as the result of co-evolution between the Asian wasp and honeybee, respectively.
- Full Text:
- Date Issued: 2007
- Authors: Tan, K , Radloff, Sarah E , Li, J J , Hepburn, H Randall , Yang, Ming-Xian , Zhang, L J , Neumann, Peter
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:6941 , http://hdl.handle.net/10962/d1011965
- Description: The vespine wasps, Vespa velutina, specialise in hawking honeybee foragers returning to their nests. We studied their behaviour in China using native Apis cerana and introduced A. mellifera colonies. When the wasps are hawking, A. cerana recruits threefold more guard bees to stave off predation than A. mellifera. The former also utilises wing shimmering as a visual pattern disruption mechanism, which is not shown by A. mellifera. A. cerana foragers halve the time of normal flight needed to dart into the nest entrance, while A. mellifera actually slows down in sashaying flight manoeuvres. V. velutina preferentially hawks A. mellifera foragers when both A. mellifera and A. cerana occur in the same apiary. The pace of wasp-hawking was highest in mid-summer but the frequency of hawking wasps was three times higher at A. mellifera colonies than at the A. cerana colonies. The wasps were taking A. mellifera foragers at a frequency eightfold greater than A. cerana foragers. The final hawking success rates of the wasps were about three times higher for A. mellifera foragers than for A. cerana. The relative success of native A. cerana over European A. mellifera in thwarting predation by the wasp V. velutina is interpreted as the result of co-evolution between the Asian wasp and honeybee, respectively.
- Full Text:
- Date Issued: 2007
Bi-Alexandroff spaces
- Authors: Matutu, Phethiwe P
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6782 , http://hdl.handle.net/10962/d1006925
- Description: Bi-Alexandroff spaces are defined as extensions of Alexandroff spaces [1]. Urysohn’s lemma for bi-Alexandroff spaces is used to show that upper and lower cozero sets of bitopological spaces are bi-Alexandroff spaces. An adjunction between bi-Alexandroff spaces and pairwise completely regular bitopological spaces is established.
- Full Text:
- Date Issued: 2007
- Authors: Matutu, Phethiwe P
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6782 , http://hdl.handle.net/10962/d1006925
- Description: Bi-Alexandroff spaces are defined as extensions of Alexandroff spaces [1]. Urysohn’s lemma for bi-Alexandroff spaces is used to show that upper and lower cozero sets of bitopological spaces are bi-Alexandroff spaces. An adjunction between bi-Alexandroff spaces and pairwise completely regular bitopological spaces is established.
- Full Text:
- Date Issued: 2007
Bioremediation of trace organic compounds found in precious metals refineries wastewaters: A review of potential options
- Barbosa, V L, Tandlich, Roman, Burgess, Jo E
- Authors: Barbosa, V L , Tandlich, Roman , Burgess, Jo E
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6469 , http://hdl.handle.net/10962/d1005798 , http://dx.doi.org/10.1016/j.chemosphere.2007.01.018 , http://www.sciencedirect.com/science/article/pii/S0045653507001026
- Description: Platinum group metal (PGM) refining processes produce large quantities of wastewater, which is contaminated with the compounds that make up the solvents/extractants mixtures used in the process. These compounds often include solvesso, β-hydroxyxime, amines, amides and methyl isobutyl ketone. A process to clean up PGM refinery wastewaters so that they could be re-used in the refining process would greatly contribute to continual water storage problems and to cost reduction for the industry. Based on the concept that organic compounds that are produced biologically can be destroyed biologically, the use of biological processes for the treatment of organic compounds in other types of waste stream has been favoured in recent years, owing to their low cost and environmental acceptability. This review examines the available biotechnologies and their effectiveness for treating compounds likely to be contained in precious metal extraction process wastewaters. The processes examined include: biofilters, fluidized bed reactors, trickle-bed bioreactors, bioscrubbers, two-phase partitioning bioreactors, membrane bioreactors and activated sludge. Although all processes examined showed adequate to excellent removal of organic compounds from various gaseous and fewer liquid waste streams, there was a variation in their effectiveness. Variations in performance of laboratory-scale biological processes are probably due to the inherent change in the microbial population composition due to selection pressure, environmental conditions and the time allowed for adaptation to the organic compounds. However, if these factors are disregarded, it can be established that activated sludge and membrane bioreactors are the most promising processes for use in the treatment of PGM refinery wastewaters.
- Full Text:
- Date Issued: 2007
- Authors: Barbosa, V L , Tandlich, Roman , Burgess, Jo E
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6469 , http://hdl.handle.net/10962/d1005798 , http://dx.doi.org/10.1016/j.chemosphere.2007.01.018 , http://www.sciencedirect.com/science/article/pii/S0045653507001026
- Description: Platinum group metal (PGM) refining processes produce large quantities of wastewater, which is contaminated with the compounds that make up the solvents/extractants mixtures used in the process. These compounds often include solvesso, β-hydroxyxime, amines, amides and methyl isobutyl ketone. A process to clean up PGM refinery wastewaters so that they could be re-used in the refining process would greatly contribute to continual water storage problems and to cost reduction for the industry. Based on the concept that organic compounds that are produced biologically can be destroyed biologically, the use of biological processes for the treatment of organic compounds in other types of waste stream has been favoured in recent years, owing to their low cost and environmental acceptability. This review examines the available biotechnologies and their effectiveness for treating compounds likely to be contained in precious metal extraction process wastewaters. The processes examined include: biofilters, fluidized bed reactors, trickle-bed bioreactors, bioscrubbers, two-phase partitioning bioreactors, membrane bioreactors and activated sludge. Although all processes examined showed adequate to excellent removal of organic compounds from various gaseous and fewer liquid waste streams, there was a variation in their effectiveness. Variations in performance of laboratory-scale biological processes are probably due to the inherent change in the microbial population composition due to selection pressure, environmental conditions and the time allowed for adaptation to the organic compounds. However, if these factors are disregarded, it can be established that activated sludge and membrane bioreactors are the most promising processes for use in the treatment of PGM refinery wastewaters.
- Full Text:
- Date Issued: 2007
Bones of contention : contestations over human remains in the Eastern Cape
- Authors: Mkhize, Nomalanga
- Date: 2007
- Subjects: Dead -- Political aspects -- South Africa Human body -- Symbolic aspects Human remains (Archaeology) -- Repatriation Human remains (Archaeology) -- Repatriation -- South Africa -- Eastern Cape Xhosa (African people) -- Social life and customs Eastern Cape (South Africa) -- Social life and customs Eastern Cape (South Africa) -- History
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2597 , http://hdl.handle.net/10962/d1007665
- Description: This thesis examines three contestations involving human remains which have arisen in the Eastern Cape over the past fifteen years. It shows that the value or meaning attached to human remains is constructed through the socio-historical dynamics out of which these contestations arise. The meaning and value of human remains is neither inherent nor neutral. In Ndancama's case, the need for housing in Fingo Village led hundreds of poor residents to settle on the township's Old Cemetery in 1972. Basic material needs trumped concerns for those buried in the cemetery. When the post-apartheid municipality sought to provide sewerage and housing infrastructure for Ndancama in 2003, its development plans were constrained by new heritage legislation which protects historic cemeteries. Residents insisted that their infrastructural needs were of primary importance. In 1993, the unearthing of human remains at the Old Military Cemetery in King William's Town created a thirteen year long saga which was only resolved with the reburial of the remains in 2006. The presence of the remains proved problematic for a number of reasons. Local authorities failed to rebury the remains speedily. The burden to store them fell on the Kaffrarian Museum which came under fire because this was considered unethical in the postapartheid era. The identity of the remains became a bone of contention in 2006 when the new Amathole District Municipality concluded that the remains were those of victims who died in the 1856-57 Great Cattle Killing. The remains and their reburial became symbols of past injustice and present restoration of African heritage. The 1996 quest by 'Nicholas Gcaleka', a 'self-styled' chief and traditional healer, to search for King Hintsa's skull in the United Kingdom provoked unprecedented public engagement with the incomplete narrative on the fate of Hintsa's body. The power to represent history, and the methods through which historical truth is discovered were at the heart of the contestation. Elites such as the Xhosa Royal and the white scientific establishment were considered neither credible nor authoritative on this historical matter. Public support for Gcaleka revealed that many South Africans sought just recompense for colonial injustices.
- Full Text:
- Date Issued: 2007
- Authors: Mkhize, Nomalanga
- Date: 2007
- Subjects: Dead -- Political aspects -- South Africa Human body -- Symbolic aspects Human remains (Archaeology) -- Repatriation Human remains (Archaeology) -- Repatriation -- South Africa -- Eastern Cape Xhosa (African people) -- Social life and customs Eastern Cape (South Africa) -- Social life and customs Eastern Cape (South Africa) -- History
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2597 , http://hdl.handle.net/10962/d1007665
- Description: This thesis examines three contestations involving human remains which have arisen in the Eastern Cape over the past fifteen years. It shows that the value or meaning attached to human remains is constructed through the socio-historical dynamics out of which these contestations arise. The meaning and value of human remains is neither inherent nor neutral. In Ndancama's case, the need for housing in Fingo Village led hundreds of poor residents to settle on the township's Old Cemetery in 1972. Basic material needs trumped concerns for those buried in the cemetery. When the post-apartheid municipality sought to provide sewerage and housing infrastructure for Ndancama in 2003, its development plans were constrained by new heritage legislation which protects historic cemeteries. Residents insisted that their infrastructural needs were of primary importance. In 1993, the unearthing of human remains at the Old Military Cemetery in King William's Town created a thirteen year long saga which was only resolved with the reburial of the remains in 2006. The presence of the remains proved problematic for a number of reasons. Local authorities failed to rebury the remains speedily. The burden to store them fell on the Kaffrarian Museum which came under fire because this was considered unethical in the postapartheid era. The identity of the remains became a bone of contention in 2006 when the new Amathole District Municipality concluded that the remains were those of victims who died in the 1856-57 Great Cattle Killing. The remains and their reburial became symbols of past injustice and present restoration of African heritage. The 1996 quest by 'Nicholas Gcaleka', a 'self-styled' chief and traditional healer, to search for King Hintsa's skull in the United Kingdom provoked unprecedented public engagement with the incomplete narrative on the fate of Hintsa's body. The power to represent history, and the methods through which historical truth is discovered were at the heart of the contestation. Elites such as the Xhosa Royal and the white scientific establishment were considered neither credible nor authoritative on this historical matter. Public support for Gcaleka revealed that many South Africans sought just recompense for colonial injustices.
- Full Text:
- Date Issued: 2007
Breaking the silence : Zanele's journey to recovery
- Authors: Payne, Charmaine
- Date: 2007
- Subjects: Rape victims -- Counseling of -- South Africa Rape victims -- Counseling of -- South Africa -- Case studies Post-traumatic stress disorder -- Patients -- Rehabilitation Post-traumatic stress disorder -- Patients -- Case studies
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3161 , http://hdl.handle.net/10962/d1007651
- Description: This study employed a case-based research design to document the psychological assessment and treatment of Zanele, a 15-year-old black Xhosa speaking female who was raped twice in 2006 by the same perpetrator. The aim of the study was to explore whether, the model for assessment and intervention for posttraumatic stress disorder (PTSD) developed by Ehlers and Clark (2000) was effective and transportable to the South African context. Zanele had a sufficient understanding of English for assessment to proceed without use of an interpreter. She reported a number of PTSD symptoms which were causing her significant distress and had impacted on her social and educational functioning. These included flashbacks of the perpetrator's face when she looked at the faces of black men, nightmares about the traumas she had endured and feeling isolated from others. A number of cognitive techniques were utilised in this study, however the central interventions included working with triggers, imagery rehearsal techniques with a focus on nightmares, and reliving with cognitive restructuring within and outside reliving. Psychoeducation and increasing her social support were also important components of the intervention. Her progress was monitored by means of several self-report measures which were displayed in graphic and tabular form. In addition, a thematically selective narrative of the assessment and first 23 sessions of the intervention was written which documents some of the central processes set in motion by the interventions. These results provide evidence that this model was both effective and transportable to the South African population. In addition, the study demonstrated that it is possible for a white English speaking clinician to work with a black Xhosa speaking individual and make substantial therapeutic gains.
- Full Text:
- Date Issued: 2007
- Authors: Payne, Charmaine
- Date: 2007
- Subjects: Rape victims -- Counseling of -- South Africa Rape victims -- Counseling of -- South Africa -- Case studies Post-traumatic stress disorder -- Patients -- Rehabilitation Post-traumatic stress disorder -- Patients -- Case studies
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3161 , http://hdl.handle.net/10962/d1007651
- Description: This study employed a case-based research design to document the psychological assessment and treatment of Zanele, a 15-year-old black Xhosa speaking female who was raped twice in 2006 by the same perpetrator. The aim of the study was to explore whether, the model for assessment and intervention for posttraumatic stress disorder (PTSD) developed by Ehlers and Clark (2000) was effective and transportable to the South African context. Zanele had a sufficient understanding of English for assessment to proceed without use of an interpreter. She reported a number of PTSD symptoms which were causing her significant distress and had impacted on her social and educational functioning. These included flashbacks of the perpetrator's face when she looked at the faces of black men, nightmares about the traumas she had endured and feeling isolated from others. A number of cognitive techniques were utilised in this study, however the central interventions included working with triggers, imagery rehearsal techniques with a focus on nightmares, and reliving with cognitive restructuring within and outside reliving. Psychoeducation and increasing her social support were also important components of the intervention. Her progress was monitored by means of several self-report measures which were displayed in graphic and tabular form. In addition, a thematically selective narrative of the assessment and first 23 sessions of the intervention was written which documents some of the central processes set in motion by the interventions. These results provide evidence that this model was both effective and transportable to the South African population. In addition, the study demonstrated that it is possible for a white English speaking clinician to work with a black Xhosa speaking individual and make substantial therapeutic gains.
- Full Text:
- Date Issued: 2007
Bridging the gap for Next Generation Services: Presence Services on Legacy Devices
- Moyo, Thamsanqa, Thinyane, Mamello, Wright, Madeleine, Irwin, Barry V W, Clayton, Peter G, Terzoli, Alfredo
- Authors: Moyo, Thamsanqa , Thinyane, Mamello , Wright, Madeleine , Irwin, Barry V W , Clayton, Peter G , Terzoli, Alfredo
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/428177 , vital:72491 , https://d1wqtxts1xzle7.cloudfront.net/49665432/Paper_2063_20-_20Moyo-libre.pdf?1476717366=andresponse-content-disposi-tion=inline%3B+filename%3DBridging_the_gap_for_Next_Generation_Ser.pdfandEx-pires=1714737455andSignature=RRbr9pzIYSYX8v7FG6FzV4tu3dFXm9qmmqq5WirOhuYdt--mjOfcDHQNLPYZHCmtgYZWdVk6bVFxfGOVJxgXrvkTe2QN2AZV3XfFTZ3mi1s3A5gw2jIXOVHrYUnaf~POgdijdY85mqWhco3vL6Qk3sOZgYjIlTF5ZGAKg1S54W978Nom01cT2~oqRA0Et6mTNmydWfF5MhFxQIq~LNmYqEqmEESKkkWQFwg6xJJUu0uGffbaZXXBA6oDI2cpfkz1FleKyKaRDRJvdfnuTHPoJJ4TzfO6DDVCWKvJ45jaxIzaGmK-03Ai29I-DPyy-c557kZh~kF3rmDg3zrXVNaL8A__andKey-Pair-Id=APKAJLOHF5GGSLRBV4ZA
- Description: Next generation services are provided by applications that leverage packet-based domains. A challenge faced by such services is the support for multiple devices, including legacy devices. Our paper examines a strategy for the pro-vision of next generation services on legacy cellular network devices. We ad-vocate that the provision of next generation services via applications on the SIM card allows for the deployment of such services on legacy devices. We demonstrate this assertion through a proof of concept application, SIMPre, that resides on a SIM card. SIMPre implements a presence service by leveraging Java Card, the SIM Application Toolkit and the OMA IMPS standard. We show that it is possible to provide a next generation service on the SIM card such that it ubiquitously integrates with the functionality of a legacy device. We con-clude through this demonstration that the SIM card is a viable option for provid-ing backward compatibility to legacy devices in the implementation of next generation services.
- Full Text:
- Date Issued: 2007
- Authors: Moyo, Thamsanqa , Thinyane, Mamello , Wright, Madeleine , Irwin, Barry V W , Clayton, Peter G , Terzoli, Alfredo
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/428177 , vital:72491 , https://d1wqtxts1xzle7.cloudfront.net/49665432/Paper_2063_20-_20Moyo-libre.pdf?1476717366=andresponse-content-disposi-tion=inline%3B+filename%3DBridging_the_gap_for_Next_Generation_Ser.pdfandEx-pires=1714737455andSignature=RRbr9pzIYSYX8v7FG6FzV4tu3dFXm9qmmqq5WirOhuYdt--mjOfcDHQNLPYZHCmtgYZWdVk6bVFxfGOVJxgXrvkTe2QN2AZV3XfFTZ3mi1s3A5gw2jIXOVHrYUnaf~POgdijdY85mqWhco3vL6Qk3sOZgYjIlTF5ZGAKg1S54W978Nom01cT2~oqRA0Et6mTNmydWfF5MhFxQIq~LNmYqEqmEESKkkWQFwg6xJJUu0uGffbaZXXBA6oDI2cpfkz1FleKyKaRDRJvdfnuTHPoJJ4TzfO6DDVCWKvJ45jaxIzaGmK-03Ai29I-DPyy-c557kZh~kF3rmDg3zrXVNaL8A__andKey-Pair-Id=APKAJLOHF5GGSLRBV4ZA
- Description: Next generation services are provided by applications that leverage packet-based domains. A challenge faced by such services is the support for multiple devices, including legacy devices. Our paper examines a strategy for the pro-vision of next generation services on legacy cellular network devices. We ad-vocate that the provision of next generation services via applications on the SIM card allows for the deployment of such services on legacy devices. We demonstrate this assertion through a proof of concept application, SIMPre, that resides on a SIM card. SIMPre implements a presence service by leveraging Java Card, the SIM Application Toolkit and the OMA IMPS standard. We show that it is possible to provide a next generation service on the SIM card such that it ubiquitously integrates with the functionality of a legacy device. We con-clude through this demonstration that the SIM card is a viable option for provid-ing backward compatibility to legacy devices in the implementation of next generation services.
- Full Text:
- Date Issued: 2007
Bridging the gap for Next Generation Services: Presence Services on Legacy Devices
- Moyo, Thamsanqa, Thinyane, Mamello, Wright, Madeleine, Irwin, Barry V W, Clayton, Peter G, Terzoli, Alfredo
- Authors: Moyo, Thamsanqa , Thinyane, Mamello , Wright, Madeleine , Irwin, Barry V W , Clayton, Peter G , Terzoli, Alfredo
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/428176 , vital:72492 , https://d1wqtxts1xzle7.cloudfront.net/49665432/Paper_2063_20-_20Moyo-libre.pdf?1476717366=andresponse-content-disposi-tion=inline%3B+filename%3DBridging_the_gap_for_Next_Generation_Ser.pdfandEx-pires=1714737455andSignature=RRbr9pzIYSYX8v7FG6FzV4tu3dFXm9qmmqq5WirOhuYdt--mjOfcDHQNLPYZHCmtgYZWdVk6bVFxfGOVJxgXrvkTe2QN2AZV3XfFTZ3mi1s3A5gw2jIXOVHrYUnaf~POgdijdY85mqWhco3vL6Qk3sOZgYjIlTF5ZGAKg1S54W978Nom01cT2~oqRA0Et6mTNmydWfF5MhFxQIq~LNmYqEqmEESKkkWQFwg6xJJUu0uGffbaZXXBA6oDI2cpfkz1FleKyKaRDRJvdfnuTHPoJJ4TzfO6DDVCWKvJ45jaxIzaGmK-03Ai29I-DPyy-c557kZh~kF3rmDg3zrXVNaL8A__andKey-Pair-Id=APKAJLOHF5GGSLRBV4ZA
- Description: Next generation services are provided by applications that leverage packet-based domains. A challenge faced by such services is the support for multiple devices, including legacy devices. Our paper examines a strategy for the pro-vision of next generation services on legacy cellular network devices. We ad-vocate that the provision of next generation services via applications on the SIM card allows for the deployment of such services on legacy devices. We demonstrate this assertion through a proof of concept application, SIMPre, that resides on a SIM card. SIMPre implements a presence service by leveraging Java Card, the SIM Application Toolkit and the OMA IMPS standard. We show that it is possible to provide a next generation service on the SIM card such that it ubiquitously integrates with the functionality of a legacy device. We con-clude through this demonstration that the SIM card is a viable option for provid-ing backward compatibility to legacy devices in the implementation of next generation services.
- Full Text:
- Date Issued: 2007
- Authors: Moyo, Thamsanqa , Thinyane, Mamello , Wright, Madeleine , Irwin, Barry V W , Clayton, Peter G , Terzoli, Alfredo
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/428176 , vital:72492 , https://d1wqtxts1xzle7.cloudfront.net/49665432/Paper_2063_20-_20Moyo-libre.pdf?1476717366=andresponse-content-disposi-tion=inline%3B+filename%3DBridging_the_gap_for_Next_Generation_Ser.pdfandEx-pires=1714737455andSignature=RRbr9pzIYSYX8v7FG6FzV4tu3dFXm9qmmqq5WirOhuYdt--mjOfcDHQNLPYZHCmtgYZWdVk6bVFxfGOVJxgXrvkTe2QN2AZV3XfFTZ3mi1s3A5gw2jIXOVHrYUnaf~POgdijdY85mqWhco3vL6Qk3sOZgYjIlTF5ZGAKg1S54W978Nom01cT2~oqRA0Et6mTNmydWfF5MhFxQIq~LNmYqEqmEESKkkWQFwg6xJJUu0uGffbaZXXBA6oDI2cpfkz1FleKyKaRDRJvdfnuTHPoJJ4TzfO6DDVCWKvJ45jaxIzaGmK-03Ai29I-DPyy-c557kZh~kF3rmDg3zrXVNaL8A__andKey-Pair-Id=APKAJLOHF5GGSLRBV4ZA
- Description: Next generation services are provided by applications that leverage packet-based domains. A challenge faced by such services is the support for multiple devices, including legacy devices. Our paper examines a strategy for the pro-vision of next generation services on legacy cellular network devices. We ad-vocate that the provision of next generation services via applications on the SIM card allows for the deployment of such services on legacy devices. We demonstrate this assertion through a proof of concept application, SIMPre, that resides on a SIM card. SIMPre implements a presence service by leveraging Java Card, the SIM Application Toolkit and the OMA IMPS standard. We show that it is possible to provide a next generation service on the SIM card such that it ubiquitously integrates with the functionality of a legacy device. We con-clude through this demonstration that the SIM card is a viable option for provid-ing backward compatibility to legacy devices in the implementation of next generation services.
- Full Text:
- Date Issued: 2007
Bycatch and discarding in the South African demersal trawl fishery
- Walmsley, Sarah A, Leslie, Rob W, Sauer, Warwick H H
- Authors: Walmsley, Sarah A , Leslie, Rob W , Sauer, Warwick H H
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123944 , vital:35518 , https://doi.10.1016/j.fishres.2007.03.002
- Description: Observers aboard commercial trawlers collected data on the total catch composition of 614 and 479 hauls made by vessels operating off the south and west coasts of South Africa, respectively. On the south coast, four fishing areas were identified on the basis of target species and fishing depth. On the west coast, hauls were separated into those targeting hake Merluccius spp. in four depth ranges (0–300, 301–400, 401–500, and >500 m) and those targeting monkfish Lophius vomerinus. For each area, the catch composition was calculated and the species assemblages were investigated using cluster analysis and multi-dimensional scaling. Finally, for each coast, the weight of fish discarded annually was estimated. On the south coast, although hake dominated, between 21% and 47% of the catch was not hake, depending on the fishing area. In comparison, hake dominated west coast catches, the proportion of hake increasing with depth. For each fishery investigated, approximately 90% of the catch was processed and landed. However, estimates of annual discards indicate that the south and west coast fisheries may annually discard 9000 or 10,000 t and 17,000 or 25,000 t, of undersized and unutilizable fish and offal, respectively, depending on the estimation method used. When developing strategies to limit or enhance utilization of bycatch, cognisance should be taken of the differences in catch composition between the south and west coasts and of the importance of bycatch revenue to south coast fishing companies.
- Full Text:
- Date Issued: 2007
- Authors: Walmsley, Sarah A , Leslie, Rob W , Sauer, Warwick H H
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123944 , vital:35518 , https://doi.10.1016/j.fishres.2007.03.002
- Description: Observers aboard commercial trawlers collected data on the total catch composition of 614 and 479 hauls made by vessels operating off the south and west coasts of South Africa, respectively. On the south coast, four fishing areas were identified on the basis of target species and fishing depth. On the west coast, hauls were separated into those targeting hake Merluccius spp. in four depth ranges (0–300, 301–400, 401–500, and >500 m) and those targeting monkfish Lophius vomerinus. For each area, the catch composition was calculated and the species assemblages were investigated using cluster analysis and multi-dimensional scaling. Finally, for each coast, the weight of fish discarded annually was estimated. On the south coast, although hake dominated, between 21% and 47% of the catch was not hake, depending on the fishing area. In comparison, hake dominated west coast catches, the proportion of hake increasing with depth. For each fishery investigated, approximately 90% of the catch was processed and landed. However, estimates of annual discards indicate that the south and west coast fisheries may annually discard 9000 or 10,000 t and 17,000 or 25,000 t, of undersized and unutilizable fish and offal, respectively, depending on the estimation method used. When developing strategies to limit or enhance utilization of bycatch, cognisance should be taken of the differences in catch composition between the south and west coasts and of the importance of bycatch revenue to south coast fishing companies.
- Full Text:
- Date Issued: 2007
Camphor-derived chiral auxiliaries: a synthetic, mechanistic and computational study
- Authors: Duggan, Andrew Robert
- Date: 2007
- Subjects: Camphor Chirality Asymmetric synthesis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4415 , http://hdl.handle.net/10962/d1006772
- Description: A broadly based approach has been undertaken to the development and use of camphor derivatives as chiral auxiliaries in asymmetric synthesis – an approach which has embraced synthetic, mechanistic and computational studies. The unambiguous characterization of mono- and dihydroxy-derivatives, obtained by reduction of chiral camphor ether dimers, has been achieved through detailed one- and two-dimensional NMR spectroscopic analysis. The resulting data has been used to establish both the regio- and stereochemistry of the hydroxyl groups. A camphor-derived cyclic iminolactone has been shown to provide a convenient platform for the synthesis of chiral α-amino acids, stereoselective monoalkylation of the iminolactone affording a range of products in yields of 52 - 65 % with up to 85 % d.e. The attempted development of chiral bifunctional Morita-Baylis-Hillman substrates has revealed an unexpected equilibration between isomeric bornane 2,3-diol monoacrylates via acid-catalysed intramolecular transesterification. A detailed [superscript 1]H NMR-based kinetic study of the rearrangement in various media and at various temperatures has permitted the determination of the kinetic and thermodynamic parameters. A computational study at the DFT level has been used to explore the potential energy surfaces of the acid-catalysed and uncatalysed transesterification of the monoacrylate esters. The theoretical data supports the involvement of cyclic intermediates and has provided a rational basis for predicting the favoured reaction pathways. Novel camphor-derived phenyl sulfonate esters and N-adamantylsulfonamides have been synthesised for use as chiral auxiliaries in the Morita-Baylis-Hillman reaction. Modeling at the Molecular Mechanics level has provided useful insights into possible conformational constraints and an adamantyl sulfonate auxiliary has been successfully used in the stereoselective synthesis of a range of products, generally in excellent yield and with up to 95 % d.e.
- Full Text:
- Date Issued: 2007
- Authors: Duggan, Andrew Robert
- Date: 2007
- Subjects: Camphor Chirality Asymmetric synthesis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4415 , http://hdl.handle.net/10962/d1006772
- Description: A broadly based approach has been undertaken to the development and use of camphor derivatives as chiral auxiliaries in asymmetric synthesis – an approach which has embraced synthetic, mechanistic and computational studies. The unambiguous characterization of mono- and dihydroxy-derivatives, obtained by reduction of chiral camphor ether dimers, has been achieved through detailed one- and two-dimensional NMR spectroscopic analysis. The resulting data has been used to establish both the regio- and stereochemistry of the hydroxyl groups. A camphor-derived cyclic iminolactone has been shown to provide a convenient platform for the synthesis of chiral α-amino acids, stereoselective monoalkylation of the iminolactone affording a range of products in yields of 52 - 65 % with up to 85 % d.e. The attempted development of chiral bifunctional Morita-Baylis-Hillman substrates has revealed an unexpected equilibration between isomeric bornane 2,3-diol monoacrylates via acid-catalysed intramolecular transesterification. A detailed [superscript 1]H NMR-based kinetic study of the rearrangement in various media and at various temperatures has permitted the determination of the kinetic and thermodynamic parameters. A computational study at the DFT level has been used to explore the potential energy surfaces of the acid-catalysed and uncatalysed transesterification of the monoacrylate esters. The theoretical data supports the involvement of cyclic intermediates and has provided a rational basis for predicting the favoured reaction pathways. Novel camphor-derived phenyl sulfonate esters and N-adamantylsulfonamides have been synthesised for use as chiral auxiliaries in the Morita-Baylis-Hillman reaction. Modeling at the Molecular Mechanics level has provided useful insights into possible conformational constraints and an adamantyl sulfonate auxiliary has been successfully used in the stereoselective synthesis of a range of products, generally in excellent yield and with up to 95 % d.e.
- Full Text:
- Date Issued: 2007
Catalytic activities of metallophthalocyanines towards detection and transformation of pollutants
- Authors: Agboola, Bolade Oyeyinka
- Date: 2007
- Subjects: Phthalocyanines Electrochemistry Pollutants -- Biodegradation Pollutants -- Measurement
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4427 , http://hdl.handle.net/10962/d1006922
- Description: Syntheses, spectral, electrochemical and spectroelectrochemical studies of new thiol-derivatised MPc complexes were satisfactorily carried out. For the first time, spectroelectrochemistry gave evidence for the formation of Ni²⁺/Ni⁺ process in a NiPc complex. Significant insights as to the nature of Fe⁺Pc and Ni⁺Pc spectra were obtained. Transformations of chlorophenols using chemical and photochemical methods are presented. For cobalt tetrasulphophthalocyanine catalysed oxidation of chlorophenols using hydrogen peroxide as the oxidant, types of oxidation products formed depended on the solvent conditions. Photolysis of aqueous solutions of chlorophenols in the presence of immobilised non-transition metal phthalocyanine photosensitisers onto Amerlite® was carried out. For the first time, MPcS[subscript mix] complexes were immobilised on Amberlite® for use in heterogeneous photocatalysis. Photolysis of the chlorophenols resulted mainly in the formation of chlorobenzoquinone derivatives. The generation of singlet oxygen (¹O₂) by these immobilised MPc photosensitisers was found to play a major role in their photoactivities. Modifications of gold electrodes with the newly synthesised thiol-derivatised MPc complexes via electropolymerisation and SAM techniques are presented. Cyclic voltammetry, impedance spectroscopy (NiPcs only) and spectroelectrochemical techniques (NiPcs only) confirmed that the complexes formed films on gold electrodes. Stable and well packed SAM films as evidenced by the voltammetric characterisation were obtained. For the first time, optimisation of the time for SAM formation based on CV technique was studied. First example of a formation of MnPc-SAM was achieved. Catalytic activities of the NiPc towards chlorophenol depended on the nature of the NiPc in the polymer films and also anti-fouling ability of the films depended on polymer film thickness. The FeTBMPc polymer modified gold electrode showed the best catalytic activity in terms of peak potential, E[subscript p] when compared to reported work in literature for nitrate electrooxidation. Cyclic voltammetry and spectroscopy studies showed that the CoPcs, FePcs and NiPcs catalysed nitrite oxidation involve 2 electrons in total while that of McPcs involve 1 electron. Better catalytic performance towards sulphite electrooxidation were obtained for the CoPcs, FePcs and MnPcs which have metal based redox processes within the range of the sulphite electrooxidation peak while the NiPcs which did not show metal based oxidation reaction performed less.
- Full Text:
- Date Issued: 2007
- Authors: Agboola, Bolade Oyeyinka
- Date: 2007
- Subjects: Phthalocyanines Electrochemistry Pollutants -- Biodegradation Pollutants -- Measurement
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4427 , http://hdl.handle.net/10962/d1006922
- Description: Syntheses, spectral, electrochemical and spectroelectrochemical studies of new thiol-derivatised MPc complexes were satisfactorily carried out. For the first time, spectroelectrochemistry gave evidence for the formation of Ni²⁺/Ni⁺ process in a NiPc complex. Significant insights as to the nature of Fe⁺Pc and Ni⁺Pc spectra were obtained. Transformations of chlorophenols using chemical and photochemical methods are presented. For cobalt tetrasulphophthalocyanine catalysed oxidation of chlorophenols using hydrogen peroxide as the oxidant, types of oxidation products formed depended on the solvent conditions. Photolysis of aqueous solutions of chlorophenols in the presence of immobilised non-transition metal phthalocyanine photosensitisers onto Amerlite® was carried out. For the first time, MPcS[subscript mix] complexes were immobilised on Amberlite® for use in heterogeneous photocatalysis. Photolysis of the chlorophenols resulted mainly in the formation of chlorobenzoquinone derivatives. The generation of singlet oxygen (¹O₂) by these immobilised MPc photosensitisers was found to play a major role in their photoactivities. Modifications of gold electrodes with the newly synthesised thiol-derivatised MPc complexes via electropolymerisation and SAM techniques are presented. Cyclic voltammetry, impedance spectroscopy (NiPcs only) and spectroelectrochemical techniques (NiPcs only) confirmed that the complexes formed films on gold electrodes. Stable and well packed SAM films as evidenced by the voltammetric characterisation were obtained. For the first time, optimisation of the time for SAM formation based on CV technique was studied. First example of a formation of MnPc-SAM was achieved. Catalytic activities of the NiPc towards chlorophenol depended on the nature of the NiPc in the polymer films and also anti-fouling ability of the films depended on polymer film thickness. The FeTBMPc polymer modified gold electrode showed the best catalytic activity in terms of peak potential, E[subscript p] when compared to reported work in literature for nitrate electrooxidation. Cyclic voltammetry and spectroscopy studies showed that the CoPcs, FePcs and NiPcs catalysed nitrite oxidation involve 2 electrons in total while that of McPcs involve 1 electron. Better catalytic performance towards sulphite electrooxidation were obtained for the CoPcs, FePcs and MnPcs which have metal based redox processes within the range of the sulphite electrooxidation peak while the NiPcs which did not show metal based oxidation reaction performed less.
- Full Text:
- Date Issued: 2007
Causes of persistent rural poverty in Thika district of Kenya, c.1953-2000
- Authors: Kinyanjui, Felistus Kinuna
- Date: 2007
- Subjects: Poverty -- Kenya -- History Rural poor -- Kenya -- History Agriculture -- Kenya -- History Kenya -- History Kenya -- Politics and government
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2547 , http://hdl.handle.net/10962/d1002399
- Description: This study investigates the causes of poverty among the residents of Thika District in Kenya over the period 1953-2000. Using the articulation of modes of production perspective, the study traces the dynamics of poverty to the geography, history and politics of Thika District. The thrust of the argument is that livelihoods in the district changed during the period under investigation, but not necessarily for the better. Landlessness, collapse of the coffee industry, intergenerational poverty, and the ravages of diseases (particularly of HIV/AIDS) are analysed. This leads to the conclusion that causes of poverty in Thika District during the period under examination were complex as one form of deprivation led to another. The study established that poverty in Thika District during the period under review was a product of a process of exclusion from the centre of political power and appropriation. While race was the basis for allocation of public resources in colonial Kenya, ethnicity has dominated the independence period. Consequently, one would have expected the residents of Thika District, the home of Kenya’s first president, Mzee Jomo Kenyatta, to have benefited inordinately from public resources during his rule. Kenyatta’s administration, however, mainly benefited the Kikuyu elite. The study therefore demonstrates that during the period under examination, the Kikuyu, like any other Kenyan community, were a heterogeneous group whose differences were accentuated by class relations. Subaltern groups in Thika District therefore benefited minimally from state patronage, just like similar groups elsewhere in rural Kenya. By the late 1970s, the level of deprivation in rural Kenya had been contained as a result of favourable prices for the country’s agricultural exports. But in the subsequent period, poverty increased under the pressures of world economic recession and slowdowns in trade. The situation was worse for Kikuyu peasants as the Second Republic of President Daniel Moi deliberately attempted undermine the Kikuyu economically. For the majority of Thika residents, this translated into further marginalisation as the Moi regime lumped them together with the Kikuyu elite who had benefitted inordinately from public resources during the Kenyatta era. This study demonstrates that no single factor can explain the prevalence of poverty in Thika District during the period under consideration. However, the poor in the district devised survival mechanisms that could be replicated elsewhere. Indeed, the dynamics of poverty in Thika District represent a microcosm not just for the broader Kenyan situation but also of rural livelihoods elsewhere in the world. The study recommends land reform and horticulture as possible ways of reducing poverty among rural communities. Further, for a successful global war on poverty there is an urgent need to have the West go beyond rhetoric and deliver on its promises to make poverty history.
- Full Text:
- Date Issued: 2007
- Authors: Kinyanjui, Felistus Kinuna
- Date: 2007
- Subjects: Poverty -- Kenya -- History Rural poor -- Kenya -- History Agriculture -- Kenya -- History Kenya -- History Kenya -- Politics and government
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2547 , http://hdl.handle.net/10962/d1002399
- Description: This study investigates the causes of poverty among the residents of Thika District in Kenya over the period 1953-2000. Using the articulation of modes of production perspective, the study traces the dynamics of poverty to the geography, history and politics of Thika District. The thrust of the argument is that livelihoods in the district changed during the period under investigation, but not necessarily for the better. Landlessness, collapse of the coffee industry, intergenerational poverty, and the ravages of diseases (particularly of HIV/AIDS) are analysed. This leads to the conclusion that causes of poverty in Thika District during the period under examination were complex as one form of deprivation led to another. The study established that poverty in Thika District during the period under review was a product of a process of exclusion from the centre of political power and appropriation. While race was the basis for allocation of public resources in colonial Kenya, ethnicity has dominated the independence period. Consequently, one would have expected the residents of Thika District, the home of Kenya’s first president, Mzee Jomo Kenyatta, to have benefited inordinately from public resources during his rule. Kenyatta’s administration, however, mainly benefited the Kikuyu elite. The study therefore demonstrates that during the period under examination, the Kikuyu, like any other Kenyan community, were a heterogeneous group whose differences were accentuated by class relations. Subaltern groups in Thika District therefore benefited minimally from state patronage, just like similar groups elsewhere in rural Kenya. By the late 1970s, the level of deprivation in rural Kenya had been contained as a result of favourable prices for the country’s agricultural exports. But in the subsequent period, poverty increased under the pressures of world economic recession and slowdowns in trade. The situation was worse for Kikuyu peasants as the Second Republic of President Daniel Moi deliberately attempted undermine the Kikuyu economically. For the majority of Thika residents, this translated into further marginalisation as the Moi regime lumped them together with the Kikuyu elite who had benefitted inordinately from public resources during the Kenyatta era. This study demonstrates that no single factor can explain the prevalence of poverty in Thika District during the period under consideration. However, the poor in the district devised survival mechanisms that could be replicated elsewhere. Indeed, the dynamics of poverty in Thika District represent a microcosm not just for the broader Kenyan situation but also of rural livelihoods elsewhere in the world. The study recommends land reform and horticulture as possible ways of reducing poverty among rural communities. Further, for a successful global war on poverty there is an urgent need to have the West go beyond rhetoric and deliver on its promises to make poverty history.
- Full Text:
- Date Issued: 2007
Changes in fuelwood use and selection following electrification in the Bushbuckridge lowveld, South Africa
- Madubansi, Mainza, Shackleton, Charlie M
- Authors: Madubansi, Mainza , Shackleton, Charlie M
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6641 , http://hdl.handle.net/10962/d1006867
- Description: Fuelwood is the primary energy source for domestic purposes throughout the developing world, in both urban and rural environments. Due to the detrimental impacts of biomass use on human and environmental health, many governments have sought to reduce its use through provision of potentially cleaner energies, of which electricity is the dominant form. Yet there are surprisingly few studies of changes in fuelwood use following the introduction of electricity, especially in rural areas of Africa. This paper reports on a longitudinal study of fuelwood use, using identical approaches, in five rural villages in the Bushbuckridge region of South Africa, spanning the period over which electricity became widely available. Almost a decade after the introduction of electricity, over 90% of households still used fuelwood for thermal purposes, especially cooking, and the mean household consumption rates over the 11-year period had not changed, even with a policy of 6 kWh per month of free electricity. The proportion of households purchasing fuelwood had increased, probably in response to a number of factors, including (i) increased fuelwood scarcity in the local environment as reflected by increased fuelwood collection times, changes in fuelwood species preferences, and ranking of scarcity by local collectors, and (ii) increases in the price of fuelwood well below that of other fuels and the prevailing inflation rate. Overall, there was an increase in the number of species harvested over the 11-year period. The implications of these findings for rural energy provision are discussed.
- Full Text:
- Date Issued: 2007
- Authors: Madubansi, Mainza , Shackleton, Charlie M
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6641 , http://hdl.handle.net/10962/d1006867
- Description: Fuelwood is the primary energy source for domestic purposes throughout the developing world, in both urban and rural environments. Due to the detrimental impacts of biomass use on human and environmental health, many governments have sought to reduce its use through provision of potentially cleaner energies, of which electricity is the dominant form. Yet there are surprisingly few studies of changes in fuelwood use following the introduction of electricity, especially in rural areas of Africa. This paper reports on a longitudinal study of fuelwood use, using identical approaches, in five rural villages in the Bushbuckridge region of South Africa, spanning the period over which electricity became widely available. Almost a decade after the introduction of electricity, over 90% of households still used fuelwood for thermal purposes, especially cooking, and the mean household consumption rates over the 11-year period had not changed, even with a policy of 6 kWh per month of free electricity. The proportion of households purchasing fuelwood had increased, probably in response to a number of factors, including (i) increased fuelwood scarcity in the local environment as reflected by increased fuelwood collection times, changes in fuelwood species preferences, and ranking of scarcity by local collectors, and (ii) increases in the price of fuelwood well below that of other fuels and the prevailing inflation rate. Overall, there was an increase in the number of species harvested over the 11-year period. The implications of these findings for rural energy provision are discussed.
- Full Text:
- Date Issued: 2007
Changing terms and conditions of employment following transferes to the Western Cape department of health
- Authors: Roman, Richard Joseph
- Date: 2007
- Subjects: Labor laws and legislation -- South Africa -- Western Cape , Industrial relations -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:10231 , http://hdl.handle.net/10948/824 , Labor laws and legislation -- South Africa -- Western Cape , Industrial relations -- South Africa -- Western Cape
- Description: This treatise describes the difficulty of changing conditions of service following transfers in terms of Section 197 of the Labour Relations Act, 66 of 1995. The Constitution of the Republic of South Africa, Act 108 of 1996, provides that ambulance services are a Provincial Legislative competence. The City of Cape Town had for many years provided ambulance services on an agency basis to the Department of Health of the Provincial Administration of the Western Cape. The impact of the constitutional provision is that those employers and employees who are performing ambulance services are not entitled anymore to perform such function. The Department of Health must take control over the ambulance services and the employees of the City of Cape Town could be transferred. The challenges facing both employers (old and new) and the trade unions to secure a transfer of the employees from the City of Cape Town to the Department of Health in terms of section 197 of the Labour Relations Act will be highlighted. It is within the context of ensuring protection of jobs whilst simultaneously effect a transfer of contracts of employment that the Labour Relations Act expressly gives various options to the parties involves in a transfer. Of the options include: • A transfer in terms of section 197(2) of the LRA where the service could be transferred from the old employer to the new employer as a going concern. This could happen without the consent of the affected employees or trade unions and collective agreements must be taken over as well, or • The new employer complies with its obligations if the transfer takes place on terms and conditions that are on the whole not less favourable to the employees what they have received by the old employer. In terms of this provision the overall package of conditions of service that was offered should not be less in comparison with that of the old employer. • The parties also have the option to negotiate a transfer agreement that could regulate the conditions of service. The focus is on the description of the conditions of service of both employers and the difficulty to persuade the trade unions to accept the Department’s conditions of service. Part of the difficulty is that the Department must obtain its mandate from the Department of the Public Service and Administration, who is also responsible for the determining of the conditions of service in the Public Service. One of the challenges is that the Department of Health cannot accommodate collective agreements of entities outside the Public Service. The City of Cape Town’s conditions of service is in the form of a collective agreement and the Labour Relations Act is very specific with the various options. These options will be discussed an analised. In conclusion recommendations are made in regard to effect a transfer of conditions of employment in terms of the Labour Relations Act to enable the Department of Health to meet its constitutional obligation.
- Full Text:
- Date Issued: 2007
- Authors: Roman, Richard Joseph
- Date: 2007
- Subjects: Labor laws and legislation -- South Africa -- Western Cape , Industrial relations -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:10231 , http://hdl.handle.net/10948/824 , Labor laws and legislation -- South Africa -- Western Cape , Industrial relations -- South Africa -- Western Cape
- Description: This treatise describes the difficulty of changing conditions of service following transfers in terms of Section 197 of the Labour Relations Act, 66 of 1995. The Constitution of the Republic of South Africa, Act 108 of 1996, provides that ambulance services are a Provincial Legislative competence. The City of Cape Town had for many years provided ambulance services on an agency basis to the Department of Health of the Provincial Administration of the Western Cape. The impact of the constitutional provision is that those employers and employees who are performing ambulance services are not entitled anymore to perform such function. The Department of Health must take control over the ambulance services and the employees of the City of Cape Town could be transferred. The challenges facing both employers (old and new) and the trade unions to secure a transfer of the employees from the City of Cape Town to the Department of Health in terms of section 197 of the Labour Relations Act will be highlighted. It is within the context of ensuring protection of jobs whilst simultaneously effect a transfer of contracts of employment that the Labour Relations Act expressly gives various options to the parties involves in a transfer. Of the options include: • A transfer in terms of section 197(2) of the LRA where the service could be transferred from the old employer to the new employer as a going concern. This could happen without the consent of the affected employees or trade unions and collective agreements must be taken over as well, or • The new employer complies with its obligations if the transfer takes place on terms and conditions that are on the whole not less favourable to the employees what they have received by the old employer. In terms of this provision the overall package of conditions of service that was offered should not be less in comparison with that of the old employer. • The parties also have the option to negotiate a transfer agreement that could regulate the conditions of service. The focus is on the description of the conditions of service of both employers and the difficulty to persuade the trade unions to accept the Department’s conditions of service. Part of the difficulty is that the Department must obtain its mandate from the Department of the Public Service and Administration, who is also responsible for the determining of the conditions of service in the Public Service. One of the challenges is that the Department of Health cannot accommodate collective agreements of entities outside the Public Service. The City of Cape Town’s conditions of service is in the form of a collective agreement and the Labour Relations Act is very specific with the various options. These options will be discussed an analised. In conclusion recommendations are made in regard to effect a transfer of conditions of employment in terms of the Labour Relations Act to enable the Department of Health to meet its constitutional obligation.
- Full Text:
- Date Issued: 2007
Characterisation of Human Hsj1a : an HSP40 molecular chaperone similar to Malarial Pfj4
- Authors: McNamara, Caryn
- Date: 2007
- Subjects: Heat shock proteins , Protein folding , Proteins -- Analysis , Proteins -- Structure , Plasmodium , Malaria , Molecular chaperones
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4083 , http://hdl.handle.net/10962/d1007603
- Description: Protein folding, translocation, oligomeric rearrangement and degradation are vital functions to obtain correctly folded proteins in any cell. The constitutive or stress-induced members of each of the heat shock protein (Hsp) families, namely Hsp70 and Hsp40, make up the Hsp70/Hsp40 chaperone system. The Hsp40 J-domain is important for the Hsp70-Hsp40 interaction and hence function. The type-II Hsp40 proteins, Homo sapiens DnaJ 1a (Hsj1a) and Plasmodium falciparum DnaJ 4 (Pfj4), are structurally similar suggesting possible similar roles during malarial infection. This thesis has focussed on identifying whether Hsj1a and Pfj4 are functionally similar in their interaction with potential partner Hsp70 chaperones. Analysis in silico also showed Pfj4 to have a potential chaperone domain, a region resembling a ubiquitin-interacting motif (UIM) corresponding to UIM1 of HsjIa, and another highly conserved region was noted between residues 232-241. The highly conserved regions within the Hsp40 J-domains, and those amino acids therein, are suggested to be responsible for mediating this Hsp70-Hsp40 partner interaction. The thermosensitive dnaJ cbpA Escherichia coli OD259 mutant strain producing type-I Agrobacterium tumefaciens DnaJ (AgtDnaJ) was used as a model heterologous expression system in this study. AgtDnaJ was able to replace the lack of two E coli Hsp40s in vivo, DnaJ and CbpA, whereas AgtDnaJ(H33Q) was unable to. AgtDnaJ-based chimeras containing the swapped J-domains of similar type-II Hsp40 proteins, namely Hsj1Agt and Pfj4Agt, were also able to replace these in E. coli OD259. Conserved J-domain amino acids were identified and were substituted in these chimeras. Of these mutant proteins, Hsj IAgt(L8A), Hsj1Agt(R24A), Hsj1Agt(H31Q), Pfj4Agt(L 11A) and Pfj4Agt(H34Q) were not able to replace the E. coli Hsp40s, whilst Pfj4Agt(Y8A) and Pfj4Agt(R27A) were only able to partially replace them. This shows the leucine of helix I and the histidine of the loop region are key in the in vivo function of both proteins and that the arginine of helix II is key for Hsj1a. The histidine-tagged Hsj1a protein was also successfully purified from the heterologous system. The in vitro stimulated ATPase activity of human Hsp70 by Hsj1a was found to be approximately 14 nmol Pí[subscript]/min/mg, and yet not stimulated by Pfj4, suggesting a possible species-specific interaction is occurring.
- Full Text:
- Date Issued: 2007
- Authors: McNamara, Caryn
- Date: 2007
- Subjects: Heat shock proteins , Protein folding , Proteins -- Analysis , Proteins -- Structure , Plasmodium , Malaria , Molecular chaperones
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4083 , http://hdl.handle.net/10962/d1007603
- Description: Protein folding, translocation, oligomeric rearrangement and degradation are vital functions to obtain correctly folded proteins in any cell. The constitutive or stress-induced members of each of the heat shock protein (Hsp) families, namely Hsp70 and Hsp40, make up the Hsp70/Hsp40 chaperone system. The Hsp40 J-domain is important for the Hsp70-Hsp40 interaction and hence function. The type-II Hsp40 proteins, Homo sapiens DnaJ 1a (Hsj1a) and Plasmodium falciparum DnaJ 4 (Pfj4), are structurally similar suggesting possible similar roles during malarial infection. This thesis has focussed on identifying whether Hsj1a and Pfj4 are functionally similar in their interaction with potential partner Hsp70 chaperones. Analysis in silico also showed Pfj4 to have a potential chaperone domain, a region resembling a ubiquitin-interacting motif (UIM) corresponding to UIM1 of HsjIa, and another highly conserved region was noted between residues 232-241. The highly conserved regions within the Hsp40 J-domains, and those amino acids therein, are suggested to be responsible for mediating this Hsp70-Hsp40 partner interaction. The thermosensitive dnaJ cbpA Escherichia coli OD259 mutant strain producing type-I Agrobacterium tumefaciens DnaJ (AgtDnaJ) was used as a model heterologous expression system in this study. AgtDnaJ was able to replace the lack of two E coli Hsp40s in vivo, DnaJ and CbpA, whereas AgtDnaJ(H33Q) was unable to. AgtDnaJ-based chimeras containing the swapped J-domains of similar type-II Hsp40 proteins, namely Hsj1Agt and Pfj4Agt, were also able to replace these in E. coli OD259. Conserved J-domain amino acids were identified and were substituted in these chimeras. Of these mutant proteins, Hsj IAgt(L8A), Hsj1Agt(R24A), Hsj1Agt(H31Q), Pfj4Agt(L 11A) and Pfj4Agt(H34Q) were not able to replace the E. coli Hsp40s, whilst Pfj4Agt(Y8A) and Pfj4Agt(R27A) were only able to partially replace them. This shows the leucine of helix I and the histidine of the loop region are key in the in vivo function of both proteins and that the arginine of helix II is key for Hsj1a. The histidine-tagged Hsj1a protein was also successfully purified from the heterologous system. The in vitro stimulated ATPase activity of human Hsp70 by Hsj1a was found to be approximately 14 nmol Pí[subscript]/min/mg, and yet not stimulated by Pfj4, suggesting a possible species-specific interaction is occurring.
- Full Text:
- Date Issued: 2007
Characterisation of InAs-based epilayers by FTIR spectroscopy
- Authors: Baisitse, Tshepiso Revonia
- Date: 2007
- Subjects: Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10534 , http://hdl.handle.net/10948/474 , Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Description: This study focuses on the characterization of InAs and InAs1-xSbx epitaxial layers by infrared reflectance and transmittance spectroscopy and Hall measurements. Reflectance measurements were performed in order to obtain the dielectric parameters and to extract from these information about the electrical properties (mobility and carrier concentration) of this important III-V material system. The transmittance measurements were used to determine the bandgap of InAsSb. Infrared reflectivity and transmittance measurements were performed in the wavelength range 200 – 2000 cm-1 on InAs and InAsSb layers grown on three types of substrates. A classical two oscillator model that takes into account both the free carriers and the lattice, was used to analyse the reflectance data using the BMDP® computer curve fitting software. The dielectric parameters and the electrical properties (carrier concentration and mobility) were extracted from the simulations. Due to the low free carrier concentration in the epitaxial structures, the plasma resonance frequency (ωp) values obtained from the simulations of reflectance spectra measured above 200 cm-1, were in the order of 20-30 cm-1. These low values were confirmed by direct measurements of ωp in reflectance spectra obtained in the range 15-200 cm-1. The simulated carrier concentration and mobility values determined optically were compared to the values determined by Hall measurements at room temperature and previously reported values by other researchers. The simulated values obtained were in reasonable agreement with the Hall values. The simulated and measured carrier concentrations obtained for InAs layers were significantly higher than the intrinsic carrier concentration for InAs at room temperature, indicating notable concentrations of donors resulting from the growth process.
- Full Text:
- Date Issued: 2007
- Authors: Baisitse, Tshepiso Revonia
- Date: 2007
- Subjects: Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10534 , http://hdl.handle.net/10948/474 , Fourier transform infrared spectroscopy , Gallium arsenide semiconductors
- Description: This study focuses on the characterization of InAs and InAs1-xSbx epitaxial layers by infrared reflectance and transmittance spectroscopy and Hall measurements. Reflectance measurements were performed in order to obtain the dielectric parameters and to extract from these information about the electrical properties (mobility and carrier concentration) of this important III-V material system. The transmittance measurements were used to determine the bandgap of InAsSb. Infrared reflectivity and transmittance measurements were performed in the wavelength range 200 – 2000 cm-1 on InAs and InAsSb layers grown on three types of substrates. A classical two oscillator model that takes into account both the free carriers and the lattice, was used to analyse the reflectance data using the BMDP® computer curve fitting software. The dielectric parameters and the electrical properties (carrier concentration and mobility) were extracted from the simulations. Due to the low free carrier concentration in the epitaxial structures, the plasma resonance frequency (ωp) values obtained from the simulations of reflectance spectra measured above 200 cm-1, were in the order of 20-30 cm-1. These low values were confirmed by direct measurements of ωp in reflectance spectra obtained in the range 15-200 cm-1. The simulated carrier concentration and mobility values determined optically were compared to the values determined by Hall measurements at room temperature and previously reported values by other researchers. The simulated values obtained were in reasonable agreement with the Hall values. The simulated and measured carrier concentrations obtained for InAs layers were significantly higher than the intrinsic carrier concentration for InAs at room temperature, indicating notable concentrations of donors resulting from the growth process.
- Full Text:
- Date Issued: 2007