Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection
- Mohammadi, Ali, Esmaeili, Farnaz, Dinarvand, Rassoul, Atyabi, Fatemeh, Walker, Roderick B
- Authors: Mohammadi, Ali , Esmaeili, Farnaz , Dinarvand, Rassoul , Atyabi, Fatemeh , Walker, Roderick B
- Date: 2010
- Language: English
- Type: Article
- Identifier: vital:6413 , http://hdl.handle.net/10962/d1006508
- Description: A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10 hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV)detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API.
- Full Text:
- Date Issued: 2010
- Authors: Mohammadi, Ali , Esmaeili, Farnaz , Dinarvand, Rassoul , Atyabi, Fatemeh , Walker, Roderick B
- Date: 2010
- Language: English
- Type: Article
- Identifier: vital:6413 , http://hdl.handle.net/10962/d1006508
- Description: A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10 hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV)detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API.
- Full Text:
- Date Issued: 2010
The determination of acetaminophen using a carbon nanotube: graphite-based electrode
- Moghaddam, Abdolmajid B, Mohammadi, Ali, Mohammadi, Somaye, Rayeji, Danyal, Dinarvand, Rassoul, Baghi, Mansoureh, Walker, Roderick B
- Authors: Moghaddam, Abdolmajid B , Mohammadi, Ali , Mohammadi, Somaye , Rayeji, Danyal , Dinarvand, Rassoul , Baghi, Mansoureh , Walker, Roderick B
- Date: 2010
- Language: English
- Type: Article , text
- Identifier: vital:6414 , http://hdl.handle.net/10962/d1006509
- Description: The oxidation of acetaminophen was studied at a glassy carbon electrode modified with multi-walled carbon nanotubes and a graphite paste. Cyclic voltammety, differential pulse voltammetry and square wave voltammetry at various pH values, scan rates, and the effect of the ratio of nanotubes to graphite were investigated in order to optimize the parameters for the determination of acetaminophen. Square wave voltammetry is the most appropriate technique in giving a characteristic peak at 0.52 V at pH 5. The porous nanostructure of the electrode improves the surface area which results in an increase in the peak current. The voltammetric response is linear in the range between 75 and 2000 ng.mL−1, with standard deviations between 0.25 and 7.8%, and a limit of detection of 25 ng.mL−1. The method has been successfully applied to the analysis of acetaminophen in tablets and biological fluids.
- Full Text:
- Date Issued: 2010
- Authors: Moghaddam, Abdolmajid B , Mohammadi, Ali , Mohammadi, Somaye , Rayeji, Danyal , Dinarvand, Rassoul , Baghi, Mansoureh , Walker, Roderick B
- Date: 2010
- Language: English
- Type: Article , text
- Identifier: vital:6414 , http://hdl.handle.net/10962/d1006509
- Description: The oxidation of acetaminophen was studied at a glassy carbon electrode modified with multi-walled carbon nanotubes and a graphite paste. Cyclic voltammety, differential pulse voltammetry and square wave voltammetry at various pH values, scan rates, and the effect of the ratio of nanotubes to graphite were investigated in order to optimize the parameters for the determination of acetaminophen. Square wave voltammetry is the most appropriate technique in giving a characteristic peak at 0.52 V at pH 5. The porous nanostructure of the electrode improves the surface area which results in an increase in the peak current. The voltammetric response is linear in the range between 75 and 2000 ng.mL−1, with standard deviations between 0.25 and 7.8%, and a limit of detection of 25 ng.mL−1. The method has been successfully applied to the analysis of acetaminophen in tablets and biological fluids.
- Full Text:
- Date Issued: 2010
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