In silico study of Plasmodium 1-deoxy-dxylulose 5-phosphate reductoisomerase (DXR) for identification of novel inhibitors from SANCDB

Diallo, Bakary N'tji

Title: In silico study of Plasmodium 1-deoxy-dxylulose 5-phosphate reductoisomerase (DXR) for identification of novel inhibitors from SANCDB
Creator: Diallo, Bakary N'tji
Subject: Plasmodium 1-deoxy-dxylulose 5-phosphate reductoisomerase
Subject: Isoprenoids
Subject: Plasmodium
Subject: Antimalarials
Subject: Malaria -- Chemotherapy
Subject: Molecules -- Models
Subject: Molecular dynamics
Subject: South African Natural Compounds Database
Date Issued: 2018
Date: 2018
Type: text
Type: Thesis
Type: Masters
Type: MSc
Identifier: http://hdl.handle.net/10962/64012
Identifier: vital:28523
Description: Malaria remains a major health concern with a complex parasite constantly developing resistance to the different drugs introduced to treat it, threatening the efficacy of the current ACT treatment recommended by WHO (World Health Organization). Different antimalarial compounds with different mechanisms of action are ideal as this decreases chances of resistance occurring. Inhibiting DXR and consequently the MEP pathway is a good strategy to find a new antimalarial with a novel mode of action. From literature, all the enzymes of the MEP pathway have also been shown to be indispensable for the synthesis of isoprenoids. They have been validated as drug targets and the X-ray structure of each of the enzymes has been solved. DXR is a protein which catalyses the second step of the MEP pathway. There are currently 255 DXR inhibitors in the Binding Database (accessed November 2017) generally based on the fosmidomycin structural scaffold and thus often showing poor drug likeness properties. This study aims to research new DXR inhibitors using in silico techniques. We analysed the protein sequence and built 3D models in close and open conformations for the different Plasmodium sequences. Then SANCDB compounds were screened to identify new potential DXR inhibitors with new chemical scaffolds. Finally, the identified hits were submitted to molecular dynamics studies, preceded by a parameterization of the manganese atom in the protein active site.
Format: 175 pages
Format: pdf
Publisher: Rhodes University
Publisher: Faculty of Science, Biochemistry and Microbiology
Language: English
Rights: Diallo, Bakary N'tji

Hits: 8138
Visitors: 8436
Downloads: 460

Collections

RU Department of Biochemistry and Microbiology (2015-)

		Thumbnail	File	Description	Size	Format
View Details Download			SOURCE1	Adobe Acrobat PDF	6 MB	Adobe Acrobat PDF	View Details Download