A broad host range reporter plasmid for the analysis of divergent promoter regions
- Authors: Jiwaji, Meesbah , Matcher, Gwynneth F , Dorrington, Rosemary A
- Date: 2008
- Language: English
- Type: Article
- Identifier: vital:6476 , http://hdl.handle.net/10962/d1006164 , http://www.scielo.org.za/scielo.php?pid=S0038-23532008000400013&script=sci_arttext
- Description: Although many vectors exist for Escherichia coli and closely related species, there are few broad host range vectors that can be conjugated into a large variety of Gram-negative bacteria. We have constructed a broad host range vector, pMJ445, that facilitates the analysis of divergent promoters in Gram-negative bacteria. The vector was validated using two intergenic regions derived from gene clusters involved in hydantoin hydrolysis, from the environmental isolates Pseudomonas putida and Agrobacterium tumefaciens. The DNA sequences analysed were capable of activating expression of the reporter enzymes, β-glucuronidase and β-galactosidase, present on pMJ445, indicating the presence of divergent promoters in the sequences selected. In addition, we demonstrated that pMJ445 can be applied to gene regulation studies.
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- Date Issued: 2008
Genetic analysis of the Octopus vulgaris population on the coast of South Africa
- Authors: Oosthuizen, Ané , Jiwaji, Meesbah , Shaw, Paul W
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6761 , http://hdl.handle.net/10962/d1007922
- Description: This study on Octopus vulgaris focused on the COIII gene region of mitochondrial DNA. Sequences from 21 samples from the Eastern Cape, and 14 samples from the Western Cape, were compared to determine whether different populations exist along the South African coast. A 380-bp segment of the COIII region of mtDNA was amplified using the polymerase chain reaction with specific designed primers. Phylogenetic inference was made using maximum parsimony (MP), maximum likelihood (ML), and distance based methods. All sequences conformed to a single haplotype. Lack of variation within and between east and west coast samples precluded further population genetic analysis. The sequence obtained in this study was also compared with other sequences lodged in the Genbank database. Phylogenetically, the South African O. vulgaris is closely related to O. vulgaris from Senegal (0.67% divergence) and the Mediterranean (1.51% divergence). Within the Mediterranean group, O. vulgaris from South Africa displayed less sequence divergence from Senegalese and Mediterranean individuals than O. vulgaris from Venezuela (3.85%) and Taiwan (3.87%). These data do not, therefore, refute the hypothesis of a single O. vulgaris genetic population around the coast.
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- Date Issued: 2004