- Title
- Preparation and characterisation of nanocomposite biodegradable films of hake fish gelatine and Na-montmorillonite
- Creator
- Mutize, Innocent
- Subject
- Nanocomposites (Materials)
- Subject
- Food -- Packaging
- Date Issued
- 2012-12
- Date
- 2012-12
- Type
- Master's theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/24261
- Identifier
- vital:62595
- Description
- Hake fish (Merluccius paradoxus) based films were prepared using gelatine extracted from hake fish bones and skins. They were characterized using FTIR, TGA and XRD. Polyethylene glycol and distilled water were used as plasticizer and solvent respectively. Montmorillonite clay was also added to the film matrix at different concentration (1-13percent gelatine weight) to improve the films‟ functional properties. Fourier Transform Infrared (FTIR), Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Ultra Violet-Visible (UV-Vis) spectroscopy analysis were used to characterise gelatine extracted from hake fish bones and skins. The extraction time and temperature were varied between 30 mins to 120 mins and 45°C to 75°C respectively. The yields of the gelatines extracted at the stated conditions were calculated from the hydroxyproline content giving values of 30 to 39percent, 43 to 55percent and 50 to 57percent for extraction at 45°C, 60°C and 75°C respectively. The effect of polyethylene glycol content on the mechanical and barrier properties of hake fish gelatine films was also investigated. The addition of polyethylene glycol to the film solution increased the water vapour permeability from 0.0321 ± 0.0011 ng.m/m2.s.Pa to 0.0993 ± 0.0007 ng.m/m2.s.Pa between 5 and 35percent polyethylene glycol content and decreased tensile strength from 41.92 ± 2.17 MPa to 29.93 ± 0.17 MPa. Sodium treated Montmorillonite was incorporated in the hake fish gelatine film solution in order to assess the effect of Montmorillonite clay on the film mechanical, barrier and thermal properties. Hake fish gelatine-montmorillonite composite films were successfully prepared and characterized. X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis indicated that the Montmorillonite clay in the film matrices was in an exfoliated state. Water vapour permeability was reduced from 0.0312 ± 0.0016 ng.m/m2.s.Pa to 0.0081 ± 0.0001ng.m/m2.s.Pa and tensile strength improved from 29.93 ± 0.17MPa to 76.78 ± 1.13 MPa.
- Description
- Thesis (MSc) -- Faculty of Science and Agriculture, 2012
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (107 leaves)
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
- Rights
- All Rights Reserved
- Rights
- Open Access
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UFH Department of Chemistry
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | M Sc (Chemistry) MUTIZE Graduation May 2013.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |