Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata)

Adeoyo, Olusegun R; Pletschke, Brett I; Dames, Joanna F

Title: Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata)
Creator: Adeoyo, Olusegun R
Creator: Pletschke, Brett I
Creator: Dames, Joanna F
Subject: To be catalogued
Date Issued: 2018
Date: 2018
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/440405
Identifier: vital:73780
Identifier: https://doi.org/10.1186/s13568-018-0685-1
Description: This study aimed to purify and characterize amyloglucosidase (AMG) from Leohumicola incrustata. AMG was purified to homogeneity from cell-free culture filtrate of an ERM fungus grown in a modified Melin–Norkrans liquid medium. The molecular mass of the AMG was estimated to be 101 kDa by combining the results of Sephadex G-100 gel filtration, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, and zymography. The Km and kcat values were 0.38 mg mL−1 and 70 s−1, respectively, using soluble starch as a substrate. The enzyme was stable at 45 °C (pH 5.0), retaining over 65% activity after a pre-incubation period of 24 h. The metal inhibition profile of the AMG showed that Mn2+ and Ca2+ enhanced activity, while it was stable to metals ions, except a few (Al3+, Co2+, Hg2+ and Cd2+) that were inhibitory at a concentration higher than 5 mM. Thin layer chromatography revealed that only glucose was produced as the product of starch hydrolysis. The amylase from L. incrustata is a glucoamylase with promising characteristics such as temperature stability over an extended period, high substrate affinity and stability to a range of chemicals. Also, this study reports for the first time the possibility of using some culturable ERM fungi to produce enzymes for the bio-economy.
Format: 10 pages
Format: pdf
Language: English
Relation: AMB Express
Relation: Adeoyo, O.R., Pletschke, B.I. and Dames, J.F., 2018. Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata). AMB Express, 8, pp.1-10
Relation: AMB Express volume 8 number 1 1 10 2018
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Springer Terms of Use Statement ( https://link.springer.com/termsandconditions)

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