The characterisation of trypanosomal type 1 DnaJ-like proteins
- Authors: Ludewig, Michael Hans
- Date: 2010
- Subjects: Molecular genetics , Molecular chaperones , Protozoa , Heat shock proteins , Trypanosoma
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4126 , http://hdl.handle.net/10962/d1015205
- Description: Trypanosomes are protozoans, of which many are parasitic, and possess complex lifecycles which alternate between mammalian and arthropod hosts. As is the case with most organisms, molecular chaperones and heat shock proteins are encoded within the genomes of these protozoans. These proteins are an integral part of maintaining the structural integrity of proteins during normal and stress conditions. Heat shock protein 40 (Hsp40) is a co-chaperone of heat shock protein 70 (Hsp70) and in some cases can act as a chaperone. These proteins work together to bind non-native polypeptide structures to prevent unfolded protein aggregrate formation in times of stress, translocate proteins across organelle membranes, and transport unsalvageable proteins to proteolytic degradation by the cellular proteasome. Hsp40s are divided into four types based on their domain structure. Analysis of the nuclear genomes of eight trypanosomatid species revealed that less than 10 of the approximate 70 Hsp40 sequences per genome were Type 1 Hsp40s, many of which contained putative orthologues in the other seven trypanosomatid genomes. One of these Type 1 Hsp40s from T b. brucei, Trypanosoma brucei DnaJ 2 (Tbj2), was functionally characterised in T brucei brucei. RNA interference knockdown of expression in T brucei brucei showed that cells deficient in Tbj2 displayed a severe inhibition of the growth of the cell population. The levels of the Tbj2 protein population in T brucei brucei cells increases after exposure to 42°c and the protein was found to have a generalized cytoplasmic subcellular localization at 37°c. These findings provide evidence that Tbj2 is an orthologue of Yeast DnaJ 1 (Y dj l), an essential S. cerevisiae protein. Hsp40s interact with their partner Hsp70s through their J-domain. The amino acids of the J-domain important for a functional interaction with Hsp70 were examined in Trypanosoma cruzi DnaJ 2 (Tcj2) (the orthologue of Tbj2) and T cruzi DnaJ protein 3 (Tcj3) by testing their ability to substitute for Y dj l in Saccharomyces cerevisae and for DnaJ in Escherichia coli. In both systems, the positively charged amino acids of Helix II and III of the J-domain disrupted the functional interaction of these Hsp40s with their partner Hsp70s. Substitutions in Helix I and IV of the J-domains of Tcj2 and Tcj3 produced varied results in the two different systems, possibly suggesting that these helices serve to define with which Hsp70s a given Hsp40 can interact. The inability of an Hsp40 and an Hsp70 to interact functionally does not necessarily mean a total absence of physical interaction between these proteins. The amino acid substitution of the histidine in the HPD motif (H34Q) of the J-domain of Tcj2 and Tcj3 removed the ability of these proteins to interact functionally with S. cerevisiae Hsp70 (Ssal) in vivo. However, preliminary binding studies using the quartz crystal microbalance with dissipation monitoring (QCM-D) show that Tcj2 and Tcj2(H34Q) both physically interact with M sativa Hsp70 in vitro. This study is the first report to provide evidence that certain trypanosoma! Type 1 Hsp40s are essential proteins. Futhermore, the interaction of these Hsp40s with Hsp70 identified important features of the functional interface of this chaperone machinery.
- Full Text:
- Date Issued: 2010
The characteristics and role of informal leaders in work groups : a South African perspective
- Authors: Wienekus, Barend Willem
- Date: 2010
- Subjects: Supervisors, industrial -- South Africa Leadership -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:1166 , http://hdl.handle.net/10962/d1002782
- Description: It has been twenty years since F W De Klerk unbanned the African National Congress. This momentous occasion changed overnight the business landscape in South Africa and the way business were done for many decades. Before and after this crucial moment in South African history, leaders played a significant role in bringing change about as well as managing it. Whether hierarchical or non-hierarchical, leadership manifests itself through all spheres of civilisation. Within any collective, formal as well as informal leadership are always at work and within the environment there always seems to be an individual that appears to hold equal or more influence and sway over the collective. This research investigates the characteristics and role of this individual, the informal leader. In addition, against the melting pot of the diversity of culture, social structures, economics, and demographics in South Africa, the influence of culture on how leadership is being perceived and experienced is also researched. The research is grounded in a post-positivists approach and conducted within a constructivist-interpretative paradigm. A qualitative approach is followed with personal interviews as the method to collect the data from respondents. The interview protocol consists of a combination of questions containing questions of both a quantitative and qualitative nature. Questions of a qualitative nature were open-ended and of an in-depth nature. The research is two pronged. The focus of the research is an Original Equipment Manufacturer (OEM) in the South African Motor Industry and for the primary goal of the research data was collected from employees within work groups of the OEM. The primary research goal investigates the role and characteristics of informal leaders in work groups as seen and experienced by their fellow employees and if there is any congruence with that of the role and characteristics of formal leaders. The study found no fundamental differences between the characteristics and role of formal and informal leaders. The characteristics and role of leaders between different cultures also appear to be the same. For the secondary research goal – determining whether the role and characteristics of informal leaders in work groups could be underpinned in the principles of Ubuntu and if consideration should be given to any cultural differences between leaders and followers by organisational hierarchies – the literature was reviewed in order to reach a conclusion with regards this goal. The literature indicates that culture does affect leadership, especially on how the leadership is executed and experienced in a multicultural society and if ignored, will have a detrimental effect on effective leadership. In order to strive towards achieving maximum productivity, it is imperative that management in South African organisations be aware of the changed dynamic within their organisations as well as on the global stage. The research therefore ends with the practical implications of informal leaders for organisations in South Africa. It is recommended that the importance and contribution of informal leaders within work groups in a multi-culture organisation needs not only to be considered as an element of group leadership, but should be accommodated by the organisation. It is also recommended that organisations recognise the cultural differences between leaders and followers in organisations and the possible consequences if ignored. If the competitive pressures and requirements of globalisation are ignored against the background of Afrocentric expectations and motivational imperatives of the South African workforce, it will result in an ineffective workforce, which will in due course render these organisations uncompetitive and non-sustainable locally and globally.
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- Date Issued: 2010
The child accused in the criminal justice system
- Authors: Brink, Ronelle Bonita
- Date: 2010
- Subjects: Children's rights -- South Africa , Children -- Legal status, laws, etc -- South Africa , Children's rights -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:10178 , http://hdl.handle.net/10948/1229 , Children's rights -- South Africa , Children -- Legal status, laws, etc -- South Africa , Children's rights -- Law and legislation -- South Africa
- Description: The high level of crime in South Africa raises the question about the failures of the criminal justice system on the one hand, and South Africa’s social policies on the other. Young people in South Africa can disproportionately be both victims and perpetrators of crime in the Republic of South Africa. The child accused in conflict with the law is dealt with in much the same way as their adult counterparts, as the criminal justice system was designed by adults for adults. South Africa became a signatory to the United Nations Convention on the Rights of the Child 19891(hereinafter referred to as UNCRC) on 16 June 1995. The UNCRC provides a backdrop to section 28 of the Constitution of the Republic of South Africa Act.2 Article 3(1) of the UNCRC provides as follows: “in all actions concerning children, whether undertaken by public or private social welfare institutions, courts of law, administrative authorities or legislative bodies, the best interests of the child shall be primary consideration.” South Africa is therefore according to article 40(3) of the UNCRC obliged to “establish laws, procedures, authorities and institutions specifically applicable to children in conflict with the law”.3 In terms of article 40(1) of the UNCRC “State Parties recognise the right of every child alleged as, accused of, or recognised as having infringed the penal law to be treated in a manner consistent with the promotion of the child’s sense of dignity and worth, which reinforces the child’s respect for the human rights and fundamental freedoms of others and which takes into account the child’s age and the desirability of promoting the child’s reintegration and the child’s assuming a constructive role in society.”4 1 Adopted by the General Assembly resolution 44/25 on 20 November 1989. 2 The Constitution of the Republic of South Africa Act, Act 108 of 1996. Hereinafter referred to as the “Constitution”. 3 South Law Reform Commission Discussion Paper 96. 4 United Nations Convention on the Rights of the Child adopted by the General Assembly resolution 44/25 on 20 November 1989. iv Synopsis 2003 states that “the Ratification of the UNCRC by the South African government in 1995 set the scene for broad-reaching policy and legislative change”.5 The Constitution includes a section protecting children’s rights, which includes the statement that children have the right not to be detained except as a measure of last resort and then for the shortest appropriate period of time, separate from adults and in conditions that take account of his/her age. 6 After being off Parliament’s agenda since 2003, the Child Justice Act7 has recently been reintroduced. The Act aims to ensure consistent, fair and appropriate treatment of the child accused in conflict with the law. The question arises whether the South African Criminal Justice system involving the child accused adequately recognises and protects the interests of the child accused, particularly in view of the present international legal position.
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- Date Issued: 2010
The Coega project: creative politicking in Post-Apartheid South Africa
- Authors: Mtimka, Ongama
- Date: 2010
- Subjects: South Africa -- Politics and government , Industrial development projects -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9425 , http://hdl.handle.net/10948/1576 , South Africa -- Politics and government , Industrial development projects -- South Africa -- Port Elizabeth
- Description: This treatise revisits the process of the implementation of the Coega Project and discusses political economic issues which emerge therein locating them in the political economic context of post-1994 South Africa. Based on an in-depth study of the “Coega Story”, and three years of observing the Coega Development Corporation engaging in the political economic space to implement the project, key themes which are relevant in understanding the nature of politics in the country are highlighted and discussed with a view to drawing lessons for future implementers of economic development projects and policy makers. Key discussions in the study include a critical analysis of the symbiotic relationship between politics and development (or broadly the economy) – where emphasis is made about the centrality of politics in implementing economic development projects; the developmental state – where key characteristics of a developmental state are highlighted; the transition from apartheid to democracy and its implications on the nature of political relations post-apartheid; industrial development as a growth strategy and the interplay of social forces in the post- 1994 political economic space. The Coega Project is located within the broader context of the ruling party seeking to advance what is called the second and, perhaps the ultimate task of the liberation struggle, socio-economic liberation. Its strategic fit in that task is discussed critically taking into account paths to industrialisation as they have been observed from Newly Industrialising Countries and South Africa’s attempts at industrialisation before and after 1994.
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- Date Issued: 2010
The depiction of female characters by male writers in selected isiXhosa drama works
- Authors: Peter, Zola Welcome
- Date: 2010
- Subjects: Xhosa drama -- 20th century -- History and criticism , Women in literature , Feminism and literature , Xhosa drama -- Male authors , Gender identity in literature
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8446 , http://hdl.handle.net/10948/1482 , Xhosa drama -- 20th century -- History and criticism , Women in literature , Feminism and literature , Xhosa drama -- Male authors , Gender identity in literature
- Description: This research expresses female character portrayal in various drama works written by males. Chapter one is a general introduction that gives the key to this study, the motivation that leads to the selection of this topic; a literary review on the portrayal of female characters in literary works written by males; the scope of study, the basic composition of the ensuing chapters and the definitions of terms that are of paramount importance for this research. Various literary theories are used in Chapter two for the analysis of the research texts. These literary theories include womanism, gender and feminism which expose the social effects caused by the negative perception of females in social life and the negative portrayal of female characters in male dramatic writings. Other literary theories include onomastics as a literary theory, which exposes the relationship between the name giver of a person and the power the name gives to its bearer, as well as psychoanalysis as a theory which proved to be unavoidable, since this study analyses the personal behaviour of the individual characters within their literary environment. Chapter three depicts the general victimization of female characters in male drama works and exposes the various effects of the attitudes of male writers towards female characters in terms of gender role. Chapter four shows a general stereotypical portrayal of female characters in male written drama texts. This chapter shows the impact of stereotyping on female characters from drama works that puts them in a vulnerable position, showing that it is risky to become a victim of ill-treatment in their communities and the literary world. Chapter five deals with the psychological literary review of female characters, showing them as being suicidal and murderers who easily take their own lives and those of other people. Chapter six is a general conclusion of the works which includes observer remarks from other literary researchers of the literature.
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- Date Issued: 2010
The design of the extensions to the Nelson Mandela Metropolitan Museum of Art with specific emphasis on the preservation and improvement of the main entrance into St. Georges Park
- Authors: Van Niekerk, Eduard Johannes
- Date: 2010
- Subjects: Art museums -- South Africa -- Port Elizabeth -- Designs and plans , Art museum architecture -- South Africa -- Port Elizabeth -- Designs and plans
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: vital:8155 , http://hdl.handle.net/10948/d1021224
- Description: The city of Port Elizabeth was established as a British colonial town in 1820 when the settlers arrived. With them, they brought ‘civilization’ from Europe, which can be seen in parts of the historic urban structure of Port Elizabeth as well the art that remains. The largest public space of the old city was St. Georges Park. The current museum buildings are part of the collection of British heritage buildings in a surrounding the park. The park is hierarchically located to align with other important places in the urban fabric. Today the park is used by city members as a sporting facility only. Club members of the various sporting facilities such as bowls, tennis, swimming and cricket clubs enjoy these amenities mostly on a Saturday. The park is also home to the famous Duckpond Cricket Pavilion designed by award winning architect John Rushmere. It is the second oldest cricket club in South Africa and has hosted various first events. The museum however has run out of space a few years ago and proposals have been formed by city officials to connect the existing gallery buildings, which would result in the closure of the gateway into the park while also disrupting the general urban flow and rhythm of the city. Finally, any new museum in South African should try to serve our diverse cultures and preserve a heritage whether pleasant or unpleasant. This type building needs to be a new type of place and be an appropriate symbol of our attitudes towards colonial heritage.
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- Date Issued: 2010
The development and implementation of an evaluation for rural ICT projects in developing countries: an exploration of the Siyakhulu Living Lab, South Africa
- Authors: Pade Khene, Caroline Ileje
- Date: 2010
- Subjects: Siyakhula Living Lab Information technology -- South Africa -- Eastern Cape Rural development projects -- South Africa -- Eastern Cape Communication in economic development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1138 , http://hdl.handle.net/10962/d1002767
- Description: Rural development is a priority for poverty alleviation and development in developing countries, as the majority of the poor live in rural areas. Information and knowledge are key strategic resources for social and economic development as they empower rural communities with the ability to expand their choices through knowing what works best in their communities. Information and communication technologies (ICT) play a significant role in supporting rural development activities through providing supportive development information and creating essential interconnectivities between rural areas and more developed regions. However, rural ICT for development (ICT4D) is still at best a ‘working hypothesis’, faced with barriers and challenges associated with implementation and use in the rural environment; which threaten the success, sustainability or relevance of an ICT intervention. Many key questions remain largely unanswered, with no concrete or credible data to support a wide range of claims concerning the use of ICT for development. The evaluation of rural ICT projects is indispensable as it determines the need, effectiveness, impact, sustainability and extent of the awareness of the contribution such projects or programmes can make in poverty alleviation and development. Even so, existing ICT4D evaluations are confrontedwith shortcomings and challenges which influence the accuracy and reliability of evaluation conclusions. These shortcomings highlight the need to embark on a more comprehensive evaluation approach, sensitive to the rural environment. This research study was aimed at developing a comprehensive rural ICT evaluation framework to assess ICT projects and interventions that work toward supporting poverty eradication in rural communities. A multi-method approach was used to determine the multiple variables and components associated with rural ICT evaluation, and then to determine how these variables interrelate. The approach is founded on programme evaluation, ICT for development evaluation, and information systems evaluation. Firstly, key domains of programme evaluations combined with an exploration of the need and shortcomings of ICT4D evaluation, contributed to the development of a template to analyse existing ICT4D evaluation frameworks and information systems frameworks, based on a selection of criteria. The combined analysis of the two groups of frameworks compares and contrasts key characteristics that form the structure of a comprehensive evaluation. This analysis and a review of programme evaluation enabled the development of a Rural ICT Comprehensive Evaluation Framework (RICT-CEF) that encompasses the key components essential for a comprehensive evaluation of rural ICT projects. The theoretical framework aims to inform ICT intervention to improve and support rural development, through the application of fundamental and interconnected evaluation domains sensitive to the rural environment, throughout the project’s lifecycle. In order to obtain a better understanding and application of the RICT-CEF, a real-life case study investigation of the Siyakhula Living Lab reveals the lessons learned (shortcomings and suitability) from applying a prototype of the framework in a rural environment. The study is characteristically a rich case study, as the investigation occurs at two levels: 1) The actual evaluation of the project to obtain results to improve or guide the project, through applying domains of the RICT-CEF, and 2) Observing and investigating the application of the RICT-CEF framework to learn lessons from its evaluation process in a real-life context. The research study reveals the compatibility of the RICT-CEF framework in a real-life rural ICT intervention case, and builds lessons learned for enhancing the framework and guiding future evaluations in ICT4D. The RICT-CEF can possibly be viewed as a platform for the key domains and processes essential for the evaluation of ICT4D interventions; which can be customised for a variety of ICT projects, such that a comparative assessment of projects can provide measurement and further awareness of the impact of rural ICT in developing countries.
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- Date Issued: 2010
The development and implementation of computer literacy terminology in isiXhosa
- Authors: Sam, Msindisi Scara
- Date: 2010
- Subjects: Xhosa language -- Data processing Computer literacy -- South Africa -- Dwesa-Cwebe Computer literacy -- South Africa -- Grahamstown Information technology -- South Africa -- Dwesa-Cwebe Information technology -- South Africa -- Grahamstown Computational linguistics -- South Africa -- Dwesa-Cwebe Computational linguistics -- South Africa -- Grahamstown Information technology -- South Africa -- Dwesa-Cwebe -- Social aspects Information technology -- South Africa -- Grahamstown -- Social aspects Digital divide -- South Africa -- Dwesa-Cwebe Communication in economic development -- South Africa -- Dwesa-Cwebe Communication in economic development -- South Africa -- Grahamstown South Africa-Norway Tertiary Education Development Programme Rhodes University. Dept. of African Languages
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3580 , http://hdl.handle.net/10962/d1002155
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- Date Issued: 2010
The development of a marketing strategy for a tannery waste by-product
- Authors: Mkhonta, Mpendulo Colin
- Date: 2010
- Subjects: Tanneries -- Waste disposal -- Marketing , Waste products -- Marketing
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8618 , http://hdl.handle.net/10948/1515 , Tanneries -- Waste disposal -- Marketing , Waste products -- Marketing
- Description: Worldwide chromium waste from tanneries is a major environmental concern. A considerable amount of effort is being implemented by tanneries to resolve the environmental concerns. Because of the wide range of chemicals used in tanning, waste treatment is an important issue in the industry. All the tanneries put considerable money and effort into treating their effluent. South African tanneries currently have various methods of disposing of their chrome waste. Most have systems for recycling the waste and disposing of it in municipal waste dumps or it is dumped on the company's own land. Hence the cost of disposal varies widely. Some companies are forced to pay excessive amounts to dispose of their waste at hazardous waste dumps. Others appear to get away by using municipal dumps. Trivalent Chrome (Pty) Ltd offers a solution to the environmental concern by extracting the most dangerous compound in the waste and converting it to a by-product (green chrome oxide). However, the company must be able to then sell of the green chromium oxide. The study seeks to address how the company can market the by-product.
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- Date Issued: 2010
The development of a technology-strategy framework to improve the competitiveness of small-to medium-sized furniture manufacturers in South Africa
- Authors: Lourens, Ann Sharon
- Date: 2010
- Subjects: Furniture industry and trade -- Effect of technological innnovations on -- South Africa , Furniture industry and trade -- Economic aspects -- South Africa , Technology strategy , Competition
- Language: English
- Type: Thesis , Doctoral , DBA
- Identifier: vital:8658 , http://hdl.handle.net/10948/1329 , Furniture industry and trade -- Effect of technological innnovations on -- South Africa , Furniture industry and trade -- Economic aspects -- South Africa , Technology strategy , Competition
- Description: The research problem addressed in this study was to establish how small- to medium-sized furniture manufacturing businesses could develop a technology strategy that would improve their competitiveness. Therefore, the primary objective of this research study was to develop a technology-strategy framework for small- to medium-sized furniture manufacturers to assist them to improve their competitiveness. Research has indicated that small- to medium-sized furniture manufacturers have the potential to contribute to South Africa‟s economic growth, job creation and gross domestic product. However, research has also indicated that small- to medium-sized furniture manufacturing industries have not kept up with technology developments and, as a result, competiveness is on the decline. Low-technology businesses, such as those found within the furniture manufacturing industry, often have no framework or methodology to guide the successful acquisition and integration of technology to enable a more competitive business. The main problem was addressed in three phases. Firstly, a literature review of strategy and technology-related factors was conducted which provided valuable insight into the development of a strategic framework. This included identification of the business strategy, external and internal factors influencing technology requirements, technology integration, -selection and criterion for competitiveness. Secondly, an empirical study consisting of both a quantitative and qualitative investigation was performed. The quantitative measuring instrument used was a questionnaire designed from the literature study information and was administered to a stratified sample of small- to medium-sized furniture manufacturers. The quantitative results were triangulated by qualitative interviews with selected respondents. The empirical results revealed a strong relationship between technology and competitiveness (as measured by business performance and technology- iv competitive advantage), thereby confirming the importance of technology utilisation for small- to medium-sized furniture manufacturers and the significance of developing a technology-strategy framework. Thirdly, by combining the literature study information and findings from the empirical investigation, the technology-strategy framework was developed. The technology-strategy framework provides a roadmap to integrate technology with the business strategy. The technology-strategy framework is used in three phases, firstly, the business strategy is identified, secondly, the technology-strategy framework provides a guide to identify and forecast technology, conduct an internal audit and identify technology that can execute the business strategy. The final phase is the application of criteria by means of which competitiveness improvement is assessed. Ultimately, the technology-strategy framework can facilitate improved competitiveness of the furniture industry.
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- Date Issued: 2010
The disparities arising in the policing of consumptive and non-consumptive marine activities
- Authors: Tembo, Danai
- Date: 2010
- Subjects: Marine resources conservation -- South Africa , Fishery law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8649 , http://hdl.handle.net/10948/1383 , Marine resources conservation -- South Africa , Fishery law and legislation -- South Africa
- Description: The South African marine environment is utilised for both consumptive and non-consumptive activities and for those activities to continue sustainably it is important that these activities be monitored and policed. South Africa's environmental legislation is structured to do just that; outlining specific regulations for all consumptive and non-consumptive activities that can be conducted in the marine environment. Some disparities have been observed regarding the policing of consumptive and non-consumptive marine activities, and these disparities were analysed by means of several case studies and semi-structured key informant interviews. Case studies covered both consumptive (abalone poaching and Illegal, Unreported and Unregulated fishing) and non-consumptive activities (the sardine run and tiger shark diving) currently being conduct in the marine environment. Key informant interviews collected opinions from legal practitioners who felt that the legislation was solid but poorly implemented; and non-consumptive operators who felt that the legislation was implemented in a way that made it difficult for non-consumptive and ecotourism activities to thrive. The poor implementation has resulted in a situation which seems to promote consumptive crimes instead of curbing them.
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- Date Issued: 2010
The dynamics of Larval fish and Zooplankton assemblages in the Sundays Estuary, South Africa
- Authors: Sutherland, Kate
- Date: 2010
- Subjects: Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10697 , http://hdl.handle.net/10948/1119 , Marine zooplankton -- South Africa , Zooplankton -- South Africa , Fishes -- Larvae -- South Africa , Estuarine ecology -- South Africa , Estuaries -- South Africa -- Management , Environmental monitoring -- South Africa , Coastal zone management -- South Africa , Estuarine area conservation -- South Africa
- Description: The larval fish and zooplankton assemblages were studied in the permanently open Sundays Estuary on the south-east coast of South Africa, using standard boat-based plankton towing methods. A total of 8174 larval and early juvenile fishes were caught, representing 12 families and 23 taxa. The Clupeidae, Gobiidae and Blenniidae were the dominant fish families. Common species included Gilchristella aestuaria, Caffrogobius gilchristi, Omobranchus woodi, Liza dumerilii, Glossogobius callidus and Myxus capensis. Estuarine resident species (Category I) predominantly in the preflexion developmental stage, dominated the system. A total of 19 zooplankton taxa were recorded. Copepoda dominated the zooplankton community. Dominant species included Pseudodiaptomus hessei, Acartia longipatella, Halicyclops sp., Mesopodopsis wooldridgei, and the larvae of Paratylodiplax edwardsii and Hymenosoma orbiculare. Mean larval fish density showed similar trends seasonally, spatially and across salinity zones, with mean zooplankton density in the Sundays Estuary. Gut content analysis of five larval fish species: Gilchristella aestuaria, Pomadasys commersonnii, Monodactylus falciformis, Myxus capensis and Rhabdosargus holubi, revealed species specific diet and prey selection. Although larval fish diet contained a variety of prey items, guts were dominated by P. hessei, chironomid larvae, Corophium triaenonyx, copepod eggs and insect larvae. Physico-chemical drivers and the interactions between these two plankton communities provide information that enables a more holistic view of the dynamics occurring in the Sundays Estuary planktonic ecosystem.
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- Date Issued: 2010
The E.coli RNA degradosome analysis of molecular chaperones and enolase
- Authors: Burger, Adélle
- Date: 2010
- Subjects: Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3950 , http://hdl.handle.net/10962/d1004009 , Molecular chaperones , Escherichia coli -- Biotechnology , Polyphosphates , Polyphosphates -- Biotechnology , RNA-protein interactions
- Description: Normal mRNA turnover is essential for genetic regulation within cells. The E. coli RNA degradosome, a large multi-component protein complex which originates through specific protein interactions, has been referred to as the “RNA decay machine” and is responsible for mRNA turnover. The degradosome functions to process RNA and its key components have been identified. The scaffold protein is RNase E and it tethers the degradosome to the cytoplasmic membrane. Polynucleotide phosphorylase (PNPase), ATP-dependent RNA helicase (RhlB helicase) and the glycolytic enzyme enolase associate with RNase E to form the degradosome. Polyphosphate kinase associates with the degradosome in substoichiometric amounts, as do the molecular chaperones DnaK and GroEL. The role of DnaK as well as that of enolase in the RNA degradosome is unknown. Very limited research has been conducted on the components of the RNA degradosome under conditions of stress. The aim of this study was to understand the role played by enolase in the assembly of the degradosome under conditions of stress, as well as investigating the protein levels of molecular chaperones under these conditions. The RNA degradosome was successfully purified through its scaffold protein using nickel-affinity chromatography. In vivo studies were performed to investigate the protein levels of DnaK and GroEL present in the degradosome under conditions of heat stress, and whether GroEL could functionally replace DnaK in the degradosome. To investigate the recruitment of enolase to the degradosome under heat stress, a subcellular fractionation was performed to determine the localization of enolase upon heat shock in vivo. The elevated temperature resulted in an increased concentration of enolase in the membrane fraction. To determine whether there is an interaction between enolase and DnaK, enolase activity assays were conducted in vitro. The effect of DnaK on enolase activity was measured upon quantifying DnaK and adding it to the enolase assays. For the first time it was observed that the activity of enolase increased with the addition of substoichiometric amounts of DnaK. This indicates that DnaK may be interacting with the RNA degradosome via enolase.
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- Date Issued: 2010
The effectiveness of human child trafficking legislation in South Africa
- Authors: Mashiyi, Tandeka
- Date: 2010
- Subjects: Human trafficking -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:10281 , http://hdl.handle.net/10948/1289 , Human trafficking -- Law and legislation -- South Africa
- Description: Trafficking in human beings is a major problem worldwide. Human trafficking is as a result of a complex set of interrelated push and pull factors. Push factors includeinter alia poverty, a lack of opportunities, dislocation of families, gender, racial and ethnic inequalities and the break-up of families. Research shows that pull factors include the promise of a better life, consumer aspirations and lack of information on the risks involved, established patterns of migration, porous borders and fewer constraints on travel. It is as a result of the global epidemic of this trafficking in persons that certain instruments on an international level as well as legislation on a national level have been enacted. The question which arises is: are these pieces of legislation effective in dealing with the scourge of human trafficking? Every legislation passed will have its strengths, as well as weaknesses but the main objective of such legislation should always be to combat, criminalise and prosecute the specific criminal act. Furthermore, the enacted legislation should be designed to effectively combat the challenges which threaten to exacerbate the criminal act. Failing to fulfil this intention will render such legislation nugatory. This treatise will be looking at various international instruments that have been passed abd v adopted by various countries, which specifically deal with trafficking in humans generally and specifically in relation to the children. International instruments that will be discussed include inter alia, slavery Convention, Convention on the Rights of the child, Worst forms or Child Labour Convention, Parlemo Protocol, United Nations Transnational Organised Crime Protocol to mention but a few. All these instruments have in a way dealt with and made provisions for the criminalisation of the act of trafficking in humans and a the scrounge of trafficking escalates the international governments strive to enact instruments that are going to be able to curtail this pandemic of trafficking. As more focus will be on the South African legislation this treatise is also going to examine all the relevant piece of legislation that have been passed by the South African government in order to deal with human trafficking. These will include the discussion of the Constitution, Child Care Act, Children’s Act, Children’s Amendment Act, Criminal Law (Sexual Offences) and Related matters. Amendment Act Prevention of Organised Crime Act as well as the Prevention and combating of Trafficking in Persons Bill. The treatise will listen critically discuss the Bill in so far as its strengths and weaknesses are concerned.
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- Date Issued: 2010
The effects of booster breaks during a sedentary night shift on physiological, psychomotor, psycho-physiological, and cognitive performance over a 3 night shift habituation phase
- Authors: Lombard, Wesley Ross
- Date: 2010
- Subjects: Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5116 , http://hdl.handle.net/10962/d1005194 , Night work , Shift systems , Performance , Exercise , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Cognition -- Effect of exercise on , Motor ability
- Description: Despite extensive research into shift work, workers working under rotating shift conditions are still plagued by the effects of the desynchronisation resulting from working against their natural circadian rhythms. Additionally, modern industries are shifting towards tasks requiring greater cognitive demand with less manual labour incorporated into the tasks. Research into operator based tasks, and hence those of a sedentary cognitive base both during day and night shifts, has been focusing on the effectiveness of the standard rest/break schedule. Research indicating that the standard rest break schedule is often ineffective in eliminating operator discomfort and performance deterioration, with these affects argued to be more pronounced during a night shift schedule. Therefore current research set out to investigate alternative rest break schedules, incorporating a short bout of physical activity and stretching exercises which are proposed to enhance performance and subjective mood, while eliminating operator discomfort for sedentary based cognitive tasks. Three conditions were tested during a three day habituation shift cycle within a laboratory, incorporating two night shift groups (control and experimental) and a control day shift group. Twelve subjects made up each group, with the two night shift groups completing the shift schedule together. The control groups followed a typical 8 hour shift schedule while the experimental group performed a booster break (exercise and stretches) activity for 7.5 minutes every hour during the night shift schedule. Over the course of the shift, subjects completed a battery of six tests providing data on physiological measurements (heart rate and temperature), performance criteria (reaction time responses, memory and neurobiological) and subjective measures. Responses obtained for all the different parameters measured indicated a strong circadian influence for the majority of the variables, indicating the course of natural down regulation within physiological and performance criteria over the night shift. The booster break significantly improved reaction time performance, subjective ratings and resulted in a high sustainable activity level. Day shift comparisons indicating that within subjective measures and reaction time performance, the booster break resulted in similar responses to those of the day shift workers, while the control night shift groups reported significantly lowers results. Additionally, the booster break had positive influences during the circadian nadir, significantly improving parameters of performance and subjective ratings of sleepiness. The results of this study indicating which variables are strong predictors and indicators of the oscillations in performance and subjective ratings due to the circadian changes. The booster break interventions had positive effects on subjective ratings and reaction time performance, while also being argued to decrease the burden placed on the cardiac system as a result of increased sympathetic tone during the night shift, while additionally resulting in similar responses to those of day shift workers. Further studies are required, however, to provide conclusive evidence particularly within a working situation over a longer shift schedule.
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- Date Issued: 2010
The effects of glove fit on task performance and on the human operator
- Authors: Stack, Jessica Danielle
- Date: 2010
- Subjects: Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5119 , http://hdl.handle.net/10962/d1005197 , Hand -- Anatomy , Hand -- Wounds and injuries , Hand -- Care and hygiene , Gloves , Safety education, Industrial , Human-machine systems , Industrial safety , Industrial accidents
- Description: The hand is one of the most complex of all of the anatomical structures in the human body. It has been found that hand injuries are among the most frequent injuries that occur to the body, predominantly during industrial activities. It has therefore been concluded that more research is needed into protective factors, such as glove use. The design features of a glove emphasise either protection or performance. There is often a trade-off between increased safety and performance capability when donning gloves. It has been determined that gloves which are fitted and comfortable for the worker may provide the best compromise between protective functions and decreased performance. This investigation aimed to assess the influence of glove fit on the performance attributes of industrial tasks, as well as on the responses of the human operator. Glove fit was analysed as 35 male participants donned three different glove sizes during each test, including a best-fitting glove, a glove one size smaller than best-fitting, and a glove one size larger than best-fitting. For each glove size, gloves of two differing materials were tested, namely nitrile and neoprene. A barehanded condition was also tested, totalling seven gloved/barehanded conditions for each test. The seven conditions were assessed in a laboratory setting in a battery of tests. This consisted of components of task performance, including maximum pulling and pushing force, maximum torque, precision of force, tactility, speed and accuracy and dexterity. The performance responses were recorded, as well as participants’ perceptual responses using the Rating of Perceived Exertion scale, and muscle activity. Six muscles were selected: Flexor Digitorum Superficialis, Flexor Pollicus Longus, Extensor Carpi Ulnaris, Extensor Carpi Radialis, Flexor Carpi Ulnaris and Flexor Carpi Radialis. The results revealed that glove fit does affect certain spects of performance, and influences human operator responses for selected task components. Furthermore, discrepancies were distinguished between orking barehanded and working with an optimally fitted glove. There was also a glove material effect established. Overall, it was found that muscle activity when exerting maximum force in a pushing and pulling direction was optimal with the nitrile glove material. Maximum torque performance was enhanced with the use of a best-fitting glove, as compared with an ill-fitting glove or barehanded work. Force precision was preferable when barehanded, as opposed to the tactility task which rendered optimal results with a best-fitting glove. The same was found for speed and accuracy results, as glove fit appeared to have no effect on performance, but performance was improved when participants were barehanded. Dexterity performance was the most conclusively influenced by the conditions, resulting in barehanded performance being optimal. However, should a glove be necessary for a given task, an optimally-fitted glove which is of a thinner material would be recommended. It is necessary to distinguish the performance components of a task within industry and select the most appropriate glove for optimal performance and the least risk of overexertion.
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- Date Issued: 2010
The effects of hyperinflation on the Zimbabwean construction industry
- Authors: Moyo, Admire
- Date: 2010
- Subjects: Inflation (Finance) -- Zimbabwe , Zimbabwe -- Economic conditions , Construction industry -- Economic aspects -- Zimbabwe
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9687 , http://hdl.handle.net/10948/1197 , Inflation (Finance) -- Zimbabwe , Zimbabwe -- Economic conditions , Construction industry -- Economic aspects -- Zimbabwe
- Description: Less than two decades ago, Zimbabwe was a symbol for the rest of the world of what Africa could become (Dell, 2005). DiSilvio (2007) contends that independent Zimbabwe was an economic success on route to attaining status of the most “developed country” in Africa. Contrary to expectations, by 2003 the Zimbabwean economy was shrinking faster than any other economy in the world at 18 percent per year (Richardson, 2005). Reports indicate that the Zimbabwean economy is in crisis and has since been set back by more than 50 years (Matikinye, 2005). This phenomenon necessitated the need for an investigation to ascertain its cause in Zimbabwe. As a result, the research identifies and presents hyperinflation as the root cause of the crisis in Zimbabwe and illustrates the validity of this assertion with a focus on the Zimbabwean construction industry. As part of the research inquiry, a review of related literature was conducted. The literature review illustrated the generic effects of hyperinflation as well as the effects of this phenomenon in action in Zimbabwe. The literature study was followed by a questionnaire survey. The questionnaire was completed by 23 contractors and 7 clients from a census of contractors and clients in Zimbabwe. The questionnaire consisted of a number of variables, which the respondents were asked to rate vis-à-vis the effects of hyperinflation in the construction industry. In summary, the questionnaire sought to determine the causes of hyperinflation in Zimbabwe, its specific effects on the construction industry and how respondents thought the phenomenon could be mitigated so as to revive the Zimbabwean construction industry. The techniques of re-scaling, in conjunction with descriptive and inferential statistics, ranking and quadrant analysis were applied to the data. Results from these analyses revealed a high degree of agreement among respondents vis-à-vis the effects of hyperinflation on the Zimbabwean construction industry. The interpretation of the results further revealed that hyperinflation has undoubtedly led to the collapse of the Zimbabwean construction industry. In conclusion, the research, applying the interpretations of v the survey findings, prescribes a number of ways in which the Zimbabwean construction sector may be resurrected. Among the recommended prescriptions, there are a number of debatable issues that arise that the researcher proposes should be subject to future study.
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- Date Issued: 2010
The evaluation of environmental reporting by publicly listed South African banks
- Authors: Oduro-Kwateng, George
- Date: 2010
- Subjects: Environmental reporting -- South Africa Banks and banking -- South Africa Social responsibility in banking -- South Africa Social responsibility of business -- South Africa Environmental protection -- South Africa Environmental economics -- South Africa Economic development -- South Africa -- Environmental aspects Environmental management -- South Africa Climatic changes -- South Africa Coalition for Environmentally Responsible Economies Global Reporting Initiative Standard Bank Limited Nedbank ABSA Bank
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:740 , http://hdl.handle.net/10962/d1003860
- Description: Recently, bankers have come to realise that banking operations, especially corporate lending, affect and are affected by the natural environment and that consequently, the banks might have an important role to play in helping to raise environmental standards. Although the environment presents significant risks to banks, in particular environmental credit risk, it also perhaps presents profitable opportunities. Stricter environmental regulations have forced companies to invest in environmentally friendly technologies and pollution control measures and in tum generated lending opportunities for bankers. This research examines the corporate practices of three of the four dominant banks in South Africa with respect to the environment, focusing on issues of climate change and environmental risk management by way of reporting and disclosure to all stakeholders. The emphasis on environmental reporting by South African banks has been reinforced by the latest release of the King III Report on Corporate Governance in South Africa. Global governance requires that the triple-bottom line should be applied in all corporate undertakings due to globalisation and trade liberalisation; however, the banking sector has responded poorly to the clarion call. The false view that the banks have no significant relationship with environmental degradation is being disproved. Environmental management is a huge and massive reconstruction of what has gone wrong with nature by human influence. The South African banks have had to face with the challenging tasks of reporting on the direct and mostly the indirect impacts of their environmental activities. Based on the three sampled banks which incidentally had greater percentages of the market capitalizations, the banks have fairly performed in environmental reporting. For example, Standard Bank (SA) Ltd has just signed the Equator Principles in 2007 implying corporate lending was done in 2007 without any respect to environmental impact assessments by corporate borrowers. Consequently, environmental reporting was not done to facilitate informed decision-making by stakeholders mostly shareholders and the communities where borrowers tun businesses. The objective of this research study is to investigate the extent and quantity of/voluntary environmental disclosures in the annual and sustainability reports of the banks listed on Johannesburg Stock Exchange. The periods examined were those subsequent to the release of the Exposure Draft Coalition for Environmentally Responsible Economies (CERES) Global Reporting Initiatives (GRI) issued in 1999. Using content analysis to focus on the environmental aspects, the research study compared three annual reports and three sustainability reports of 2007 year for the three sampled banks in order to evaluate reporting practices in the period surrounding this intervention. The results suggest a trend to triple bottom-line reporting and the extent and quantity of environmental information, albeit in specific categories.
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- Date Issued: 2010
The experience of instant messaging upon adolescent female relationship
- Authors: Eberhardt, Antoinette
- Date: 2010
- Subjects: Adolescent psychology -- South Africa , Instant messaging -- South Africa , Friendship in adolescence -- South Africa , Teenage girls -- Social networks , Social networks -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9877 , http://hdl.handle.net/10948/1324 , Adolescent psychology -- South Africa , Instant messaging -- South Africa , Friendship in adolescence -- South Africa , Teenage girls -- Social networks , Social networks -- Psychological aspects
- Description: Social Interaction Technologies (SIT) have broadened the horizon of communication in terms of the way people are able to communicate. It is now possible to interact with others across the world and engage in numerous activities ranging from dating to political movements, hobbies and even professions (Chigona, Chigona, Ngqokelela, & Mpofu, 2009). Adolescents and pre-adolescents especially are inclined to make use of SIT in their social lives with the most popular mode of communication, apart from email, being instant messaging (IM) (Brown, Mounts, Lamborn, & Steinberg, 1993; Bryant, Sanders-Jackson, & Smallwood, 2006; Madden & Rainie, 2003). Adolescents tend to use IM regularly as a tool to maintain relationships and girls especially, use it as a tool to socialise (Jennings & Wartella, 2004; Lenhart, Rainie, & Lewis, 2001). The mobile phone or cell phone, which is another example of an SIT-based communication, has become an established medium of technical, social and commercial communication in South Africa. It has given rise to the development and vast growth of a mobile youth culture who consider it an essential tool for communicating (Bosch, 2008). In South Africa, instant messages may be sent via mobile phone using one of two methods: MXit and the SMS (short messaging service). MXit and the SMS are considered convenient tools of communication as an ongoing conversation in the form of a text message may be maintained in the present (Yoshii, Matsuda, Habuchi, Dobashi, Iwata, & Kin, 2002).
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- Date Issued: 2010
The fatigue life cycle prediction of a light aircraft undercarriage
- Authors: Erasmus, Daniel Jacobus
- Date: 2010
- Subjects: Metals--Fatigue
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9614 , http://hdl.handle.net/10948/1527 , Metals--Fatigue
- Description: The reliability of systems relies heavily on accurate fatigue life prediction of related components. Fatigue life prediction is a complicated process requiring the correct methodology to determine accurate and reliable predictions. The Palmgren – Miner damage accumulation hypothesis is widely used in determining the fatigue life of components exposed to variable loading conditions. Modifications have been made to this hypothesis trying to achieve a greater degree of accuracy, of these the Liu – Zenner modification has been the most successful. In this report the systematic process of fatigue life prediction using the Liu – Zenner modification to achieve reliable results is calculated. A representative stress time history measured in service on the component forms the basis for defining a flight cycle which is the chosen unit in which to express the fatigue life. Rainflow cycle counting performed on the stress time history allowed the formulation of a load spectrum to which the component is exposed in one cycle. Combining the load spectrum with the developed SN curve of the component and using the Liu – Zenner modification to the Palmgren – Miner rule a reliable fatigue life in cycles is predicted.
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- Date Issued: 2010