Economic impact studies and methodological bias : the case of the National Arts Festival in South Africa
- Authors: Bragge, Brent Reuben
- Date: 2011
- Subjects: Standard Bank National Arts Festival Standard Bank National Arts Festival -- Economic aspects Performing arts festivals- Economic aspects -- South Africa Arts -- Economic aspects -- South Africa Economic impact analysis Edinburgh International Festival -- Economic aspects Klein Karoo Nasionale Kunstefees (Oudtshoorn, South Africa) -- Economic aspects Volksbladfees (Bloemfontein, South Africa) -- Economic aspects
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:968 , http://hdl.handle.net/10962/d1002702
- Description: Over the course of the last three decades, it has become popular practice to evaluate tourism events like cultural festivals in financial terms, through the use of economic impact studies. This can be attributed at least in part to the notable growth in the number of festivals being held globally and, as such, a higher level of competition between festivals for the limited funding which is available. Economic impact studies, and the resultant findings, have thus become powerful tools for the lobbying of sponsorship, and it has become increasingly important that the impact calculations be as accurate as possible, so as to effectively allocate both government and private resources to projects which will be of the greatest benefit to the host region. The allocation of funding is especially vital in an area like the Eastern Cape Province of South Africa, which is faced with many financial difficulties. The allocation of public funds to an event like the National Arts Festival, which is hosted in a relatively wealthy part of the province, might be weighed against initiatives which directly benefit the poorer parts of the region. Although it is acknowledged that the benefits which are felt by the host community of a cultural event go beyond that of the financial, it is often on this basis that festivals are most easily compared. The primary goal of the thesis was to analyse the various forms of methodological bias which can exist in the economic impact analyses (EIA) associated with cultural events. Theoretical considerations were discussed, specifically regarding economic impact as a method of measuring value. Various forms of bias (including data collection, the calculation of visitor numbers, multipliers, defining the area of interest, inclusion of visitor spending, and accounting for benefits only, not costs) are put into a real-life context, through the investigation of economic impact studies conducted on three selected South African festivals (the Volksblad, the Klein Karoo Nasionale Kunstfees, and the National Arts Festival), and one international festival (the Edinburgh Festival). An in-depth comparison of two separate studies conducted at the National Arts Festival (NAF) in 2004 (by Antrobus and Snowball) and 2005 (by Saayman et al.) was made, focussing on the manner in which the economic impact was calculated. Having considered the common forms of bias, and assessing several possible reasons for the difference of approximately twenty million Rand in the advertised economic impacts, it was concluded that, most likely, the miscalculation of visitor numbers was the cause. This was confirmed when the Antrobus and Saayman methods were applied to the 2006 NAF data, and noting that the economic impact figures arrived at were strikingly similar. As such, it is advisable that extreme caution be taken when calculating visitor numbers, as they can significantly influence the outcome of an economic impact study. It is recommended that each study should also have transparent checks in place, regarding the key calculation figures, to ensure that less scrupulous researchers are not as easily able to succumb to the pressure event sponsors might impose to produce inflated impact values.
- Full Text:
- Date Issued: 2011
- Authors: Bragge, Brent Reuben
- Date: 2011
- Subjects: Standard Bank National Arts Festival Standard Bank National Arts Festival -- Economic aspects Performing arts festivals- Economic aspects -- South Africa Arts -- Economic aspects -- South Africa Economic impact analysis Edinburgh International Festival -- Economic aspects Klein Karoo Nasionale Kunstefees (Oudtshoorn, South Africa) -- Economic aspects Volksbladfees (Bloemfontein, South Africa) -- Economic aspects
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:968 , http://hdl.handle.net/10962/d1002702
- Description: Over the course of the last three decades, it has become popular practice to evaluate tourism events like cultural festivals in financial terms, through the use of economic impact studies. This can be attributed at least in part to the notable growth in the number of festivals being held globally and, as such, a higher level of competition between festivals for the limited funding which is available. Economic impact studies, and the resultant findings, have thus become powerful tools for the lobbying of sponsorship, and it has become increasingly important that the impact calculations be as accurate as possible, so as to effectively allocate both government and private resources to projects which will be of the greatest benefit to the host region. The allocation of funding is especially vital in an area like the Eastern Cape Province of South Africa, which is faced with many financial difficulties. The allocation of public funds to an event like the National Arts Festival, which is hosted in a relatively wealthy part of the province, might be weighed against initiatives which directly benefit the poorer parts of the region. Although it is acknowledged that the benefits which are felt by the host community of a cultural event go beyond that of the financial, it is often on this basis that festivals are most easily compared. The primary goal of the thesis was to analyse the various forms of methodological bias which can exist in the economic impact analyses (EIA) associated with cultural events. Theoretical considerations were discussed, specifically regarding economic impact as a method of measuring value. Various forms of bias (including data collection, the calculation of visitor numbers, multipliers, defining the area of interest, inclusion of visitor spending, and accounting for benefits only, not costs) are put into a real-life context, through the investigation of economic impact studies conducted on three selected South African festivals (the Volksblad, the Klein Karoo Nasionale Kunstfees, and the National Arts Festival), and one international festival (the Edinburgh Festival). An in-depth comparison of two separate studies conducted at the National Arts Festival (NAF) in 2004 (by Antrobus and Snowball) and 2005 (by Saayman et al.) was made, focussing on the manner in which the economic impact was calculated. Having considered the common forms of bias, and assessing several possible reasons for the difference of approximately twenty million Rand in the advertised economic impacts, it was concluded that, most likely, the miscalculation of visitor numbers was the cause. This was confirmed when the Antrobus and Saayman methods were applied to the 2006 NAF data, and noting that the economic impact figures arrived at were strikingly similar. As such, it is advisable that extreme caution be taken when calculating visitor numbers, as they can significantly influence the outcome of an economic impact study. It is recommended that each study should also have transparent checks in place, regarding the key calculation figures, to ensure that less scrupulous researchers are not as easily able to succumb to the pressure event sponsors might impose to produce inflated impact values.
- Full Text:
- Date Issued: 2011
Economics of land reform models used in Mashonaland Central Province of Zimbabwe
- Authors: Musemwa, Lovemore
- Date: 2011
- Subjects: Land reform -- Zimbabwe , Agricultural productivity -- Zimbabwe , Land tenure -- Zimbabwe , Infrastructure (Economics) -- Government policy -- Zimbabwe , Land reform beneficiaries -- Zimbabwe , Land use -- Economic aspects -- Zimbabwe , Field crops -- Zimbabwe , Data envelopment analysis -- Zimbabwe , Land settlement -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD (Agricultural Economics)
- Identifier: vital:11168 , http://hdl.handle.net/10353/435 , Land reform -- Zimbabwe , Agricultural productivity -- Zimbabwe , Land tenure -- Zimbabwe , Infrastructure (Economics) -- Government policy -- Zimbabwe , Land reform beneficiaries -- Zimbabwe , Land use -- Economic aspects -- Zimbabwe , Field crops -- Zimbabwe , Data envelopment analysis -- Zimbabwe , Land settlement -- Zimbabwe
- Description: The land reform that has unfolded in Zimbabwe since 1980 used different models and had diverse consequences. Since the implementation of the fast tract land reform programme in 2000, Zimbabwe experienced heavy reduction in yield and output at farm level that led to a 70% shortfall in production to meet annual food requirements (Richardson, 2005). The economic crisis in Zimbabwe has been characterized by worsening food insecurity especially in the rural areas where harvests continue to be poor. In the beef sector, Zimbabwe has failed to meet its export quota to the EU. The shortfall in production to meet annual food requirements shows a very grim situation but do not tell us about the performance of resettled farmers who now occupy much of the productive land. The broad objective of the study was to determine and compare the production efficiency of resettled farmers in Zimbabwe across land reform models. In addition, the study determined land use intensity. The study was conducted in the Mashonaland Central Province of Zimbabwe mainly because a wide variety of field crops were grown by resettled farmers. The respondents were stratified into three groups. These were: beneficiaries of land reform before 2000 (resettle scheme), fast track A1 model and fast track A2 model. The three models differ on how they were implemented and supported and this might result in different efficiencies of the models. A total of 245 copies structured questionnaire were administered on the resettled farmers from June to September 2010. Descriptive statistics was applied to the basic characteristics of the sampled households. The effect of model of land reform, gender of the household head, marital status, age of the household head, education, household size, religion, dependence ratio, whether the farmer was fulltime or part-time in farming, experience of the farmers in farming at that environment, total land size owned by the farmers and soil type on revenue per hectare and land use rate were determined using the GLM procedure of SAS (2003). Significance differences between least-square group means were compared using the PDIFF test of SAS (2003). The relationship between Revenue and land utilization was examined using the Pearson‟s correlations analysis. Dependance between response variables that had an effect on either revenue per hectare or land utilization with all the other response variables was tested using the Chi-square test for dependance. To find the effect of arable land used and herd size on revenue per hectare and land use the RSREG Procedure of SAS (2003) was used. Input oriented DEA model under the assumption of constant return to scale was used to estimate efficiency in this study. To identify factors that influence efficiency, a Tobit model censored at zero was selected. The mean land use rate varied significantly (p<0.05) with the land reform model with A2 having highest land use rate of 67%. The A1 and old resettlement households had land use rates of 53% and 46%, respectively. Sex, marital status, age of the household head, education and household size significantly affected land use (P<0.05). Revenue per hectare was not affected by any the factors that were inputted in the model. Results from the DEA approach showed that A2 farmers (large land owners) had an average technical efficiency score of 0.839, while the lowest ranking model (A1) had an average score of 0.618. Small land holders (A1 and the old resettled farmers) are on average less cost-efficient than large land owners, with a score of 0.29 for the former compared with 0.45 for the latter. From the factors that were entered in the Tobit model, age of household head, excellent production knowledge and farmer status affected technical efficiency whereas allocative efficiency was only affected by good production knowledge, farm size, arable land owned and area under cultivation. Factors which affected economic efficiency of the resettled farmers are secondary education, household size, farm size, cultivated area and arable land owned. None of the included socio-economic variables has significant effects on the allocative and economic efficiency of the resettled farmers. Thus, the allocative and economic inefficiencies of the farmers might be accounted for by other natural and environmental factors which were not captured in the model.
- Full Text:
- Date Issued: 2011
- Authors: Musemwa, Lovemore
- Date: 2011
- Subjects: Land reform -- Zimbabwe , Agricultural productivity -- Zimbabwe , Land tenure -- Zimbabwe , Infrastructure (Economics) -- Government policy -- Zimbabwe , Land reform beneficiaries -- Zimbabwe , Land use -- Economic aspects -- Zimbabwe , Field crops -- Zimbabwe , Data envelopment analysis -- Zimbabwe , Land settlement -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD (Agricultural Economics)
- Identifier: vital:11168 , http://hdl.handle.net/10353/435 , Land reform -- Zimbabwe , Agricultural productivity -- Zimbabwe , Land tenure -- Zimbabwe , Infrastructure (Economics) -- Government policy -- Zimbabwe , Land reform beneficiaries -- Zimbabwe , Land use -- Economic aspects -- Zimbabwe , Field crops -- Zimbabwe , Data envelopment analysis -- Zimbabwe , Land settlement -- Zimbabwe
- Description: The land reform that has unfolded in Zimbabwe since 1980 used different models and had diverse consequences. Since the implementation of the fast tract land reform programme in 2000, Zimbabwe experienced heavy reduction in yield and output at farm level that led to a 70% shortfall in production to meet annual food requirements (Richardson, 2005). The economic crisis in Zimbabwe has been characterized by worsening food insecurity especially in the rural areas where harvests continue to be poor. In the beef sector, Zimbabwe has failed to meet its export quota to the EU. The shortfall in production to meet annual food requirements shows a very grim situation but do not tell us about the performance of resettled farmers who now occupy much of the productive land. The broad objective of the study was to determine and compare the production efficiency of resettled farmers in Zimbabwe across land reform models. In addition, the study determined land use intensity. The study was conducted in the Mashonaland Central Province of Zimbabwe mainly because a wide variety of field crops were grown by resettled farmers. The respondents were stratified into three groups. These were: beneficiaries of land reform before 2000 (resettle scheme), fast track A1 model and fast track A2 model. The three models differ on how they were implemented and supported and this might result in different efficiencies of the models. A total of 245 copies structured questionnaire were administered on the resettled farmers from June to September 2010. Descriptive statistics was applied to the basic characteristics of the sampled households. The effect of model of land reform, gender of the household head, marital status, age of the household head, education, household size, religion, dependence ratio, whether the farmer was fulltime or part-time in farming, experience of the farmers in farming at that environment, total land size owned by the farmers and soil type on revenue per hectare and land use rate were determined using the GLM procedure of SAS (2003). Significance differences between least-square group means were compared using the PDIFF test of SAS (2003). The relationship between Revenue and land utilization was examined using the Pearson‟s correlations analysis. Dependance between response variables that had an effect on either revenue per hectare or land utilization with all the other response variables was tested using the Chi-square test for dependance. To find the effect of arable land used and herd size on revenue per hectare and land use the RSREG Procedure of SAS (2003) was used. Input oriented DEA model under the assumption of constant return to scale was used to estimate efficiency in this study. To identify factors that influence efficiency, a Tobit model censored at zero was selected. The mean land use rate varied significantly (p<0.05) with the land reform model with A2 having highest land use rate of 67%. The A1 and old resettlement households had land use rates of 53% and 46%, respectively. Sex, marital status, age of the household head, education and household size significantly affected land use (P<0.05). Revenue per hectare was not affected by any the factors that were inputted in the model. Results from the DEA approach showed that A2 farmers (large land owners) had an average technical efficiency score of 0.839, while the lowest ranking model (A1) had an average score of 0.618. Small land holders (A1 and the old resettled farmers) are on average less cost-efficient than large land owners, with a score of 0.29 for the former compared with 0.45 for the latter. From the factors that were entered in the Tobit model, age of household head, excellent production knowledge and farmer status affected technical efficiency whereas allocative efficiency was only affected by good production knowledge, farm size, arable land owned and area under cultivation. Factors which affected economic efficiency of the resettled farmers are secondary education, household size, farm size, cultivated area and arable land owned. None of the included socio-economic variables has significant effects on the allocative and economic efficiency of the resettled farmers. Thus, the allocative and economic inefficiencies of the farmers might be accounted for by other natural and environmental factors which were not captured in the model.
- Full Text:
- Date Issued: 2011
Ecotherapy in post-conflict healing: a study of the experiences of ex-combatants in the Eastern Cape township of Mdantsane
- Authors: Mbona, Sifingo
- Date: 2011
- Subjects: Post-traumatic stress disorder , Veterans -- Services for , Human services -- South Africa -- Eastern Cape , Environmental psychology
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11758 , http://hdl.handle.net/10353/331 , Post-traumatic stress disorder , Veterans -- Services for , Human services -- South Africa -- Eastern Cape , Environmental psychology
- Description: During this research project, an attempt was made to understand the role of ecotherapy as a therapeutic model in post conflict healing. The aim was to evaluate the application and significance of ecotherapy as an intervention strategy for post conflict healing for individuals who were exposed to violent encounters during the liberation struggle. Eight respondents (involved in the arms struggle) from Mdanstane Township in the Eastern Cape, were taken through a series of workshops as part of an ecotherapy healing process. The research concluded that all the participants had suffered from some form of traumatic stress because of their exposure to violence and violent experiences. Moreover, on closer examination the situation of ex-combatants proved to be more complex and often volatile, due to the absence of social support, loss of social status, absence of economic, employment opportunities, and the dislocation from their families and communities. Subsequent to the ecotherapy intervention, a positive outlook amongst the participants was recorded. Furthermore, there was an indication of a significant increase in their sense of self-worth and personal development, which influenced the manner in which the participants viewed the world, related to their families or communities and responded to life in general. Essentially, the research findings, conclusions, and recommendations contributed towards a better understanding of ex-combatants and the value that could be derived from ecotherapy as a post conflict healing method for individuals and groups exposed to violence and trauma.
- Full Text:
- Date Issued: 2011
- Authors: Mbona, Sifingo
- Date: 2011
- Subjects: Post-traumatic stress disorder , Veterans -- Services for , Human services -- South Africa -- Eastern Cape , Environmental psychology
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11758 , http://hdl.handle.net/10353/331 , Post-traumatic stress disorder , Veterans -- Services for , Human services -- South Africa -- Eastern Cape , Environmental psychology
- Description: During this research project, an attempt was made to understand the role of ecotherapy as a therapeutic model in post conflict healing. The aim was to evaluate the application and significance of ecotherapy as an intervention strategy for post conflict healing for individuals who were exposed to violent encounters during the liberation struggle. Eight respondents (involved in the arms struggle) from Mdanstane Township in the Eastern Cape, were taken through a series of workshops as part of an ecotherapy healing process. The research concluded that all the participants had suffered from some form of traumatic stress because of their exposure to violence and violent experiences. Moreover, on closer examination the situation of ex-combatants proved to be more complex and often volatile, due to the absence of social support, loss of social status, absence of economic, employment opportunities, and the dislocation from their families and communities. Subsequent to the ecotherapy intervention, a positive outlook amongst the participants was recorded. Furthermore, there was an indication of a significant increase in their sense of self-worth and personal development, which influenced the manner in which the participants viewed the world, related to their families or communities and responded to life in general. Essentially, the research findings, conclusions, and recommendations contributed towards a better understanding of ex-combatants and the value that could be derived from ecotherapy as a post conflict healing method for individuals and groups exposed to violence and trauma.
- Full Text:
- Date Issued: 2011
Educating users about information security by means of game play
- Authors: Monk, Thomas Philippus
- Date: 2011
- Subjects: Computer security , Educational games -- Design , Computer networks -- Security measures
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9748 , http://hdl.handle.net/10948/1493 , Computer security , Educational games -- Design , Computer networks -- Security measures
- Description: Information is necessary for any business to function. However, if one does not manage one’s information assets properly then one’s business is likely to be at risk. By implementing Information Security controls, procedures, and/or safeguards one can secure information assets against risks. The risks of an organisation can be mitigated if employees implement safety measures. However, employees are often unable to work securely due to a lack of knowledge. This dissertation evaluates the premise that a computer game could be used to educate employees about Information Security. A game was developed with the aim of educating employees in this regard. If people were motivated to play the game, without external motivation from an organisation, then people would also, indirectly, be motivated to learn about Information Security. Therefore, a secondary aim of this game was to be self-motivating. An experiment was conducted in order to test whether or not these aims were met. The experiment was conducted on a play test group and a control group. The play test group played the game before completing a questionnaire that tested the information security knowledge of participants, while the control group simply completed the questionnaire. The two groups’ answers were compared in order to obtain results. This dissertation discusses the research design of the experiment and also provides an analysis of the results. The game design will be discussed which provides guidelines for future game designers to follow. The experiment indicated that the game is motivational, but perhaps not educational enough. However, the results suggest that a computer game can still be used to teach users about Information Security. Factors that involved consequence and repetition contributed towards the educational value of the game, whilst competitiveness and rewards contributed to the motivational aspect of the game.
- Full Text:
- Date Issued: 2011
- Authors: Monk, Thomas Philippus
- Date: 2011
- Subjects: Computer security , Educational games -- Design , Computer networks -- Security measures
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9748 , http://hdl.handle.net/10948/1493 , Computer security , Educational games -- Design , Computer networks -- Security measures
- Description: Information is necessary for any business to function. However, if one does not manage one’s information assets properly then one’s business is likely to be at risk. By implementing Information Security controls, procedures, and/or safeguards one can secure information assets against risks. The risks of an organisation can be mitigated if employees implement safety measures. However, employees are often unable to work securely due to a lack of knowledge. This dissertation evaluates the premise that a computer game could be used to educate employees about Information Security. A game was developed with the aim of educating employees in this regard. If people were motivated to play the game, without external motivation from an organisation, then people would also, indirectly, be motivated to learn about Information Security. Therefore, a secondary aim of this game was to be self-motivating. An experiment was conducted in order to test whether or not these aims were met. The experiment was conducted on a play test group and a control group. The play test group played the game before completing a questionnaire that tested the information security knowledge of participants, while the control group simply completed the questionnaire. The two groups’ answers were compared in order to obtain results. This dissertation discusses the research design of the experiment and also provides an analysis of the results. The game design will be discussed which provides guidelines for future game designers to follow. The experiment indicated that the game is motivational, but perhaps not educational enough. However, the results suggest that a computer game can still be used to teach users about Information Security. Factors that involved consequence and repetition contributed towards the educational value of the game, whilst competitiveness and rewards contributed to the motivational aspect of the game.
- Full Text:
- Date Issued: 2011
Effect of alkaline pre-treatments on the synergistic enzymatic hydrolysis of sugarcane (Saccharum officinarum) bagasse by Clostridium cellulovorans XynA, ManA and ArfA
- Authors: Beukes, Natasha
- Date: 2011
- Subjects: Sugarcane -- Biotechnology Lignocellulose -- Biotechnology Renewable energy sources Hydrolysis Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3952 , http://hdl.handle.net/10962/d1004011
- Description: The continual increase in industrialization and global population has increased the dependency and demand on traditional fossil fuels for energy; however, there are limited amounts of fossil fuels available. The slow depletion of fossil fuels has sparked a fresh interest in renewable sources such as lignocellulose to produce a variety of biofuels, such as biogases (e.g. methane), bioethanol, biodiesel and a variety of other solvents and economically valuable by-products. Agricultural crop wastes produced in surplus are typically lignocellulosic in composition and thus partially recalcitrant to enzymatic degradation. The recalcitrant nature of plant biomass and the inability to obtain complete enzymatic hydrolysis has led to the establishment of various pre-treatment strategies. Alkaline pre-treatments increase the accessibility of the exposed surface to enzymatic hydrolysis through the removal of acetyl and uronic acid substituents on hemicellulose. Unlike the use of steam and acid pre-treatments, alkaline pre-treatments solubilize lignin and a small percentage of the hemicellulose, increasing enzyme accessibility and thus the hydrolysis of lignocellulose. The majority of Clostridium cellulovorans associated enzyme synergy studies have been devoted to an understanding of the cellulolytic and hemi-cellulolytic degradation of plant cell walls. However, little is known about the effect of various physical and chemical pre-treatments on the synergistic enzymatic degradation of plant biomass and possible depolymerization of plant cell walls. This study investigates the use of slake lime, sodium hydroxide and ammonium hydroxide to pre-treat sugarcane bagasse under mild conditions and elucidates potentially important synergistic associations between the C. cellulovorans enzymes for the enhanced degradation of lignocellulose. The primary aims of the study were addressed using of a variety of techniques. This included suitable vector constructs for the expression and purification of recombinant C. cellulovorans enzymes, identification of the effects of various pre-treatments on enzyme synergy, and identification of the resultant reducing sugars and phenolic compounds (released during the pre-treatment of the bagasse). This study also made use of physical and chemical pre-treatment methods, protein purification using affinity, high performance liquid and thin layer chromatography, mass spectrometry, sodium dodecyl sulphate and fluorophore-assisted polyacrylamide gel electrophoresis (FACE) , enzymatic degradation and synergy studies with various substrates indirectly using the 3, 4-dinitrosalicylic acid (DNS) reducing sugar assay. From this investigation, the following conclusions were made: alkaline pre-treatment successfully solublised, redistributed and removed lignin from the bagasse, increasing the digestibility of the substrates. In summary, the most effective pre-treatment employed 0.114 M ammonium hydroxide / gram bagasse at 70°C for 36 hours, followed by hydrolysis with an enzyme cocktail containing 25% ManA and 75% XynA. This increased the production of sugars approximately 13-fold. Analysis of the sugars produced by the synergistic hydrolysis of sugarcane bagasse (SCB) indicated the presence of xylose, indicating that the enzymes are potentially bifunctional under certain conditions. This study indicated that the use of mild pre-treatment conditions sufficiently removed a large portion of lignin without affecting the hemicellulose moiety of the SCB. This facilitated the potential use of the hemicellulose component for the production of valuable products (e.g. xylitol) in addition to the production of bioethanol. Thus, the potential use of additional components of holocellulose may generate an additional biotechnological benefit and allow a certain degree of flexibility in the biofuel industry, depending on consumer and industrial needs.
- Full Text:
- Date Issued: 2011
- Authors: Beukes, Natasha
- Date: 2011
- Subjects: Sugarcane -- Biotechnology Lignocellulose -- Biotechnology Renewable energy sources Hydrolysis Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3952 , http://hdl.handle.net/10962/d1004011
- Description: The continual increase in industrialization and global population has increased the dependency and demand on traditional fossil fuels for energy; however, there are limited amounts of fossil fuels available. The slow depletion of fossil fuels has sparked a fresh interest in renewable sources such as lignocellulose to produce a variety of biofuels, such as biogases (e.g. methane), bioethanol, biodiesel and a variety of other solvents and economically valuable by-products. Agricultural crop wastes produced in surplus are typically lignocellulosic in composition and thus partially recalcitrant to enzymatic degradation. The recalcitrant nature of plant biomass and the inability to obtain complete enzymatic hydrolysis has led to the establishment of various pre-treatment strategies. Alkaline pre-treatments increase the accessibility of the exposed surface to enzymatic hydrolysis through the removal of acetyl and uronic acid substituents on hemicellulose. Unlike the use of steam and acid pre-treatments, alkaline pre-treatments solubilize lignin and a small percentage of the hemicellulose, increasing enzyme accessibility and thus the hydrolysis of lignocellulose. The majority of Clostridium cellulovorans associated enzyme synergy studies have been devoted to an understanding of the cellulolytic and hemi-cellulolytic degradation of plant cell walls. However, little is known about the effect of various physical and chemical pre-treatments on the synergistic enzymatic degradation of plant biomass and possible depolymerization of plant cell walls. This study investigates the use of slake lime, sodium hydroxide and ammonium hydroxide to pre-treat sugarcane bagasse under mild conditions and elucidates potentially important synergistic associations between the C. cellulovorans enzymes for the enhanced degradation of lignocellulose. The primary aims of the study were addressed using of a variety of techniques. This included suitable vector constructs for the expression and purification of recombinant C. cellulovorans enzymes, identification of the effects of various pre-treatments on enzyme synergy, and identification of the resultant reducing sugars and phenolic compounds (released during the pre-treatment of the bagasse). This study also made use of physical and chemical pre-treatment methods, protein purification using affinity, high performance liquid and thin layer chromatography, mass spectrometry, sodium dodecyl sulphate and fluorophore-assisted polyacrylamide gel electrophoresis (FACE) , enzymatic degradation and synergy studies with various substrates indirectly using the 3, 4-dinitrosalicylic acid (DNS) reducing sugar assay. From this investigation, the following conclusions were made: alkaline pre-treatment successfully solublised, redistributed and removed lignin from the bagasse, increasing the digestibility of the substrates. In summary, the most effective pre-treatment employed 0.114 M ammonium hydroxide / gram bagasse at 70°C for 36 hours, followed by hydrolysis with an enzyme cocktail containing 25% ManA and 75% XynA. This increased the production of sugars approximately 13-fold. Analysis of the sugars produced by the synergistic hydrolysis of sugarcane bagasse (SCB) indicated the presence of xylose, indicating that the enzymes are potentially bifunctional under certain conditions. This study indicated that the use of mild pre-treatment conditions sufficiently removed a large portion of lignin without affecting the hemicellulose moiety of the SCB. This facilitated the potential use of the hemicellulose component for the production of valuable products (e.g. xylitol) in addition to the production of bioethanol. Thus, the potential use of additional components of holocellulose may generate an additional biotechnological benefit and allow a certain degree of flexibility in the biofuel industry, depending on consumer and industrial needs.
- Full Text:
- Date Issued: 2011
Effect of BT maize on earthworm activity, silage quality and residue decomposition in the central Eastern Cape
- Authors: Kamota, Agathar
- Date: 2011
- Subjects: Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11863 , http://hdl.handle.net/10353/413 , Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Description: There are concerns that genetic modification of maize with Bacillus thuringiensis may influence its structural and chemical composition which, together with the Cry1Ab proteins, may affect agro-ecosystem processes and feed quality. This study was aimed at investigating the expression of Cry1Ab protein in leaves, stems and roots of Bt maize and the effect of genetic modification (MON810) on activity of earthworms, silage quality and decomposition of residues in the field. In 2009/10 four maize cultivars; DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777 were planted. Expression of Cry1Ab in leaves, stems and roots was analyzed at 6, 8, 10 and 12 weeks after planting (WAP). Earthworms were also sampled from the same treatments at 6, 9 and 18 WAP. Two silage experiments were conducted using maize cultivars, DKC80-12B and DKC80-10 produced in the 2008/09 season and DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777, produced in 2009/10. The silage was sampled at 0, 2, 4, 8, 15 and 42 days in 2008/09 and 0, 8 and 42 days in 2009/10 and analyzed for Ash Free Dry Matter, Crude Fiber, Neutral Detergent Fiber, Acid Detergent Fiber, Acid Detergent Lignin, Crude Protein and Total Digestible Nutrients. Two litter-bag decomposition studies were also carried out (i) in 2008 (surface applied) using maize cultivars DKC80-12B, DKC80-10 and DKC6-125 residues and (ii) in 2009 (soil incorporated) using DKC75-15B, CRN3505, PAN6Q-321B v and PAN6Q-121. Ash-free dry matter and Cry1Ab protein were measured throughout the incubation time. There were no differences between DKC61-25B and PAN6Q-321B in terms of expression of Cry1Ab in leaves, stems and roots over time. The Cry1Ab expression levels were in decreasing order: leaves > stems > roots. No effects of Bt maize on earthworm numbers and biomass were observed. There were no differences in all silages parameters except NDF and ADF, which were higher in the Bt maize silage than that of the non-Bt maize from the 2008/09 season. The Cry1Ab levels were essentially not reduced during ensiling. The maize residues (both Bt and non-Bt maize) degraded to similar levels, either when surface-applied or incorporated into soil but soil-incorporated residues decomposed faster than surface-applied ones. Cry1Ab degraded as the plant matrix decomposed. The findings suggested that maize genetically modified with the Bt MON810 event can be grown in the Central Eastern Cape without affecting earthworm numbers and biomass, silage quality and decomposition of maize residues.
- Full Text:
- Date Issued: 2011
- Authors: Kamota, Agathar
- Date: 2011
- Subjects: Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11863 , http://hdl.handle.net/10353/413 , Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Description: There are concerns that genetic modification of maize with Bacillus thuringiensis may influence its structural and chemical composition which, together with the Cry1Ab proteins, may affect agro-ecosystem processes and feed quality. This study was aimed at investigating the expression of Cry1Ab protein in leaves, stems and roots of Bt maize and the effect of genetic modification (MON810) on activity of earthworms, silage quality and decomposition of residues in the field. In 2009/10 four maize cultivars; DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777 were planted. Expression of Cry1Ab in leaves, stems and roots was analyzed at 6, 8, 10 and 12 weeks after planting (WAP). Earthworms were also sampled from the same treatments at 6, 9 and 18 WAP. Two silage experiments were conducted using maize cultivars, DKC80-12B and DKC80-10 produced in the 2008/09 season and DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777, produced in 2009/10. The silage was sampled at 0, 2, 4, 8, 15 and 42 days in 2008/09 and 0, 8 and 42 days in 2009/10 and analyzed for Ash Free Dry Matter, Crude Fiber, Neutral Detergent Fiber, Acid Detergent Fiber, Acid Detergent Lignin, Crude Protein and Total Digestible Nutrients. Two litter-bag decomposition studies were also carried out (i) in 2008 (surface applied) using maize cultivars DKC80-12B, DKC80-10 and DKC6-125 residues and (ii) in 2009 (soil incorporated) using DKC75-15B, CRN3505, PAN6Q-321B v and PAN6Q-121. Ash-free dry matter and Cry1Ab protein were measured throughout the incubation time. There were no differences between DKC61-25B and PAN6Q-321B in terms of expression of Cry1Ab in leaves, stems and roots over time. The Cry1Ab expression levels were in decreasing order: leaves > stems > roots. No effects of Bt maize on earthworm numbers and biomass were observed. There were no differences in all silages parameters except NDF and ADF, which were higher in the Bt maize silage than that of the non-Bt maize from the 2008/09 season. The Cry1Ab levels were essentially not reduced during ensiling. The maize residues (both Bt and non-Bt maize) degraded to similar levels, either when surface-applied or incorporated into soil but soil-incorporated residues decomposed faster than surface-applied ones. Cry1Ab degraded as the plant matrix decomposed. The findings suggested that maize genetically modified with the Bt MON810 event can be grown in the Central Eastern Cape without affecting earthworm numbers and biomass, silage quality and decomposition of maize residues.
- Full Text:
- Date Issued: 2011
Effect of nanoparticles on the photophysicochemical behaviour of metallophthalocyanines
- Moeno, Sharon Keitumetse Gail Mpheletso
- Authors: Moeno, Sharon Keitumetse Gail Mpheletso
- Date: 2011 , 2011-03-30
- Subjects: Phthalocyanines Nanoparticles Photochemistry Quantum dots
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4310 , http://hdl.handle.net/10962/d1004968
- Description: The synthesis, spectroscopic characterization, and studies of the photophysicochemical behaviour of selective anionic, cationic and neutral metallophthallocyanine (MPc) complexes were carried out and the results are presented herein. Studies on the effect of the central metal ion, the solvent used and the presence of nanoparticles on the photophysicochemical properties were conducted. The findings showed that the photophysicochemical parameters were mostly enhanced in the presence of central metal ions of high atomic numbers and also in the presence of nanoparticles. It was also observed that solvents that encouraged the monomericity of the MPc complexes also lead to improved photophysical and photochemical behaviour. CdTe quantum dots (QDs) stabilized with mercaptocarbonic acids were also observed to cause stimulated emission of the MPcs through Förster resonance energy transfer (FRET) thus acting as energy donors while the respective MPc acted as energy acceptors in all the FRET studies. FRET was observed following the photoexcitation of QDs for all monomeric anionic MPcs but it was also shown to occur for some cationic MPcs in organic media. Both the substituent and solvent used were found to exert a strong influence on the occurrence of FRET. Other cationic MPcs however showed different behaviour in the presence of the meraptocarbonic stabilized CdTe QDs; with the cationic porphyrazine giving clear indications of Pc ring reduction. The rest of the cationic MPcs did not give clear evidence of Pc ring reduction, instead they showed signs of aggregate formation possibly from the assembly of electrostatic ion pair complexes which could result in reduction of the quaternized pyridinium ring of the substituent. Both the QDs and the MPc complex emission spectra were significantly quenched for each in the presence of the other. Stern-Volmer quenching studies indicated that both static and dynamic quenching of the QDs in the presence of MPcs took place. The fluorescence lifetimes of the mercaptopropionic acid (MPA) capped CdTe QDs in the presence of various MPc complexes showed quenching of mostly the longer lifetimes of the QDs in the presence of MPcs suggesting that the surface defects and states are involved in the interaction of the QDs and MPcs. An MPc complex terminating in thio tethers was employed in the conjugation to AuNPs. Spectroscopic and microscopic studies confirmed the formation of the MPc-AuNP conjugate which was also shown to exhibit improved photophysicochemical properties compared to the free MPc.
- Full Text:
- Date Issued: 2011
- Authors: Moeno, Sharon Keitumetse Gail Mpheletso
- Date: 2011 , 2011-03-30
- Subjects: Phthalocyanines Nanoparticles Photochemistry Quantum dots
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4310 , http://hdl.handle.net/10962/d1004968
- Description: The synthesis, spectroscopic characterization, and studies of the photophysicochemical behaviour of selective anionic, cationic and neutral metallophthallocyanine (MPc) complexes were carried out and the results are presented herein. Studies on the effect of the central metal ion, the solvent used and the presence of nanoparticles on the photophysicochemical properties were conducted. The findings showed that the photophysicochemical parameters were mostly enhanced in the presence of central metal ions of high atomic numbers and also in the presence of nanoparticles. It was also observed that solvents that encouraged the monomericity of the MPc complexes also lead to improved photophysical and photochemical behaviour. CdTe quantum dots (QDs) stabilized with mercaptocarbonic acids were also observed to cause stimulated emission of the MPcs through Förster resonance energy transfer (FRET) thus acting as energy donors while the respective MPc acted as energy acceptors in all the FRET studies. FRET was observed following the photoexcitation of QDs for all monomeric anionic MPcs but it was also shown to occur for some cationic MPcs in organic media. Both the substituent and solvent used were found to exert a strong influence on the occurrence of FRET. Other cationic MPcs however showed different behaviour in the presence of the meraptocarbonic stabilized CdTe QDs; with the cationic porphyrazine giving clear indications of Pc ring reduction. The rest of the cationic MPcs did not give clear evidence of Pc ring reduction, instead they showed signs of aggregate formation possibly from the assembly of electrostatic ion pair complexes which could result in reduction of the quaternized pyridinium ring of the substituent. Both the QDs and the MPc complex emission spectra were significantly quenched for each in the presence of the other. Stern-Volmer quenching studies indicated that both static and dynamic quenching of the QDs in the presence of MPcs took place. The fluorescence lifetimes of the mercaptopropionic acid (MPA) capped CdTe QDs in the presence of various MPc complexes showed quenching of mostly the longer lifetimes of the QDs in the presence of MPcs suggesting that the surface defects and states are involved in the interaction of the QDs and MPcs. An MPc complex terminating in thio tethers was employed in the conjugation to AuNPs. Spectroscopic and microscopic studies confirmed the formation of the MPc-AuNP conjugate which was also shown to exhibit improved photophysicochemical properties compared to the free MPc.
- Full Text:
- Date Issued: 2011
Effect of turbidity on the foraging success of Glossogobius callidus (Teleostei: Gobiidae)
- Parkinson, Matthew C, Booth, Anthony J
- Authors: Parkinson, Matthew C , Booth, Anthony J
- Date: 2011
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124375 , vital:35600 , https://doi.org/10.2989/16085914.2011.589120
- Description: Particles suspended in water attenuate the light that passes through it via absorption or scattering (Utne-Palm 2002). For example, whereas algae absorb specific wavelengths of light necessary for photosynthesis, inorganic suspensoids tend to scatter light (Radke and Gaupisch 2005). This results in lowered ambient light levels and reduces the visual acuity of aquatic organisms. From a fish’s perspective, an increase in water turbidity has the potential to alter visually-mediated reproductive behaviours (Järvenpää and Lindström 2004), predator avoidance (Meager et al. 2006) and foraging success negatively (Utne-Palm 2002, Rowe et al. 2003, Sweka and Hartman 2003, Stuart-Smith et al. 2004). The foraging success of various fish species was affected by increased turbidity through decreasing predator–prey encounter rates (Sweka and Hartman 2003) and through decreasing visual acuity whereby the distance at which prey are visually detected is reduced (Utne-Palm 2002).
- Full Text:
- Date Issued: 2011
- Authors: Parkinson, Matthew C , Booth, Anthony J
- Date: 2011
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124375 , vital:35600 , https://doi.org/10.2989/16085914.2011.589120
- Description: Particles suspended in water attenuate the light that passes through it via absorption or scattering (Utne-Palm 2002). For example, whereas algae absorb specific wavelengths of light necessary for photosynthesis, inorganic suspensoids tend to scatter light (Radke and Gaupisch 2005). This results in lowered ambient light levels and reduces the visual acuity of aquatic organisms. From a fish’s perspective, an increase in water turbidity has the potential to alter visually-mediated reproductive behaviours (Järvenpää and Lindström 2004), predator avoidance (Meager et al. 2006) and foraging success negatively (Utne-Palm 2002, Rowe et al. 2003, Sweka and Hartman 2003, Stuart-Smith et al. 2004). The foraging success of various fish species was affected by increased turbidity through decreasing predator–prey encounter rates (Sweka and Hartman 2003) and through decreasing visual acuity whereby the distance at which prey are visually detected is reduced (Utne-Palm 2002).
- Full Text:
- Date Issued: 2011
Effectiveness of ICT education in the Northern school area of Port Elizabeth
- Authors: Mooi, Alicia Tenjiswa
- Date: 2011
- Subjects: Educational technology -- South Africa -- Eastern Cape , Computer-assisted instruction -- South Africa -- Eastern Cape , Educational innovations -- Technological innovations -- South Africa -- Eastern Cape , Communication -- Technological innovations
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9049 , http://hdl.handle.net/10948/1605 , Educational technology -- South Africa -- Eastern Cape , Computer-assisted instruction -- South Africa -- Eastern Cape , Educational innovations -- Technological innovations -- South Africa -- Eastern Cape , Communication -- Technological innovations
- Description: The use of information communication technology in the 21st Century is very important. The skills and knowledge of using these technologies should start at high school level, as it is at this stage where learners are equipped for tertiary education and the business world. This study seeks to answer how much ICT is used at the average school in Port Elizabeth and how effective such use is. South Africa is one of the countries in the whole world that is still behind in using the new technology. Learners from high school still enter university with no knowledge of how to use a computer. This becomes a problem in the world of technology as almost everything is done using technology. It also becomes a big problem when learners look for jobs in the business world. An active learning theory has been used on the study because, if learners can learn and have access computer at school, life will be easy for both learners and teachers, there would be co-operation and discussion and the fast way of doing school work. For the purpose of the study a qualitative research method has been used to ensure that answers are received straight from, teachers and learners for evidence. It is therefore clear that there are schools in South Africa that still needs the attention of the government in terms of getting access to computers and to have professional teachers for teaching the subject. The government has a responsibility of making sure that all learners and teachers in high schools have access to computers and internet. This will improve the working and learning conditions of both parties involved.
- Full Text:
- Date Issued: 2011
- Authors: Mooi, Alicia Tenjiswa
- Date: 2011
- Subjects: Educational technology -- South Africa -- Eastern Cape , Computer-assisted instruction -- South Africa -- Eastern Cape , Educational innovations -- Technological innovations -- South Africa -- Eastern Cape , Communication -- Technological innovations
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9049 , http://hdl.handle.net/10948/1605 , Educational technology -- South Africa -- Eastern Cape , Computer-assisted instruction -- South Africa -- Eastern Cape , Educational innovations -- Technological innovations -- South Africa -- Eastern Cape , Communication -- Technological innovations
- Description: The use of information communication technology in the 21st Century is very important. The skills and knowledge of using these technologies should start at high school level, as it is at this stage where learners are equipped for tertiary education and the business world. This study seeks to answer how much ICT is used at the average school in Port Elizabeth and how effective such use is. South Africa is one of the countries in the whole world that is still behind in using the new technology. Learners from high school still enter university with no knowledge of how to use a computer. This becomes a problem in the world of technology as almost everything is done using technology. It also becomes a big problem when learners look for jobs in the business world. An active learning theory has been used on the study because, if learners can learn and have access computer at school, life will be easy for both learners and teachers, there would be co-operation and discussion and the fast way of doing school work. For the purpose of the study a qualitative research method has been used to ensure that answers are received straight from, teachers and learners for evidence. It is therefore clear that there are schools in South Africa that still needs the attention of the government in terms of getting access to computers and to have professional teachers for teaching the subject. The government has a responsibility of making sure that all learners and teachers in high schools have access to computers and internet. This will improve the working and learning conditions of both parties involved.
- Full Text:
- Date Issued: 2011
Effectiveness of integrated quality management systems for teachers in the department of education: Butterworth district
- Authors: Sihlali, Mzuzile
- Date: 2011
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:8273 , http://hdl.handle.net/10948/d1015984
- Description: The purpose of this research was to investigate the effectiveness of IQMS amongst teachers in the Butterworth district. Using a convenient sample of 79 senior secondary school teachers, a survey research design was used to collect both quantitative as well as qualitative data on the effectiveness of IQMS. The formulation of statements on the effectiveness of IQMS was informed by the levels of effectiveness of the Kirkpatrick’s model. A likert scale was used to rate the effectiveness of IQMS with respect to teachers’ views on the various statements that sought to measure the effectiveness of IQMS. The narratives of teachers on the SWOT analyses constituted qualitative data which was used to corroborate and contradict trends from the quantitative findings. Thus, SWOT analysis results were used to validate quantitative findings. In analysing quantitative data, descriptive statistics were employed to look at the distribution of the responses and overall trends. Significant differences in terms of gender, age, employment status, length of service, and the highest tertiary qualification were computed by the SPSS software. The SWOT analyses results from the open-ended questions were analysed through extraction of themes, categories and patterns. The quantitative findings suggested that IQMS is an effective evaluation instrument though the qualitative SWOT analyses results suggested otherwise. The study’s findings highlighted areas where IQMS is potentially effective, areas where there are challenges in the implementation of IQMS, and niches for intervention. The study has implications for IQMS policy and programme administrators in the district offices, school principals and teachers in the school sites, as well as implications for further research.
- Full Text:
- Date Issued: 2011
- Authors: Sihlali, Mzuzile
- Date: 2011
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:8273 , http://hdl.handle.net/10948/d1015984
- Description: The purpose of this research was to investigate the effectiveness of IQMS amongst teachers in the Butterworth district. Using a convenient sample of 79 senior secondary school teachers, a survey research design was used to collect both quantitative as well as qualitative data on the effectiveness of IQMS. The formulation of statements on the effectiveness of IQMS was informed by the levels of effectiveness of the Kirkpatrick’s model. A likert scale was used to rate the effectiveness of IQMS with respect to teachers’ views on the various statements that sought to measure the effectiveness of IQMS. The narratives of teachers on the SWOT analyses constituted qualitative data which was used to corroborate and contradict trends from the quantitative findings. Thus, SWOT analysis results were used to validate quantitative findings. In analysing quantitative data, descriptive statistics were employed to look at the distribution of the responses and overall trends. Significant differences in terms of gender, age, employment status, length of service, and the highest tertiary qualification were computed by the SPSS software. The SWOT analyses results from the open-ended questions were analysed through extraction of themes, categories and patterns. The quantitative findings suggested that IQMS is an effective evaluation instrument though the qualitative SWOT analyses results suggested otherwise. The study’s findings highlighted areas where IQMS is potentially effective, areas where there are challenges in the implementation of IQMS, and niches for intervention. The study has implications for IQMS policy and programme administrators in the district offices, school principals and teachers in the school sites, as well as implications for further research.
- Full Text:
- Date Issued: 2011
Effects of Acacia karroo supplementation on the quality of meat from Xhosa lopeared goats
- Authors: Ngambu, Simthembile
- Date: 2011
- Subjects: Goats -- Nutrition -- South Africa , Food -- Sensory evaluation , Goat meat -- South Africa , Goats -- Feeding and feeds -- South Africa , Meat -- Quality -- South Africa , Goats -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11808 , http://hdl.handle.net/10353/450 , Goats -- Nutrition -- South Africa , Food -- Sensory evaluation , Goat meat -- South Africa , Goats -- Feeding and feeds -- South Africa , Meat -- Quality -- South Africa , Goats -- South Africa -- Eastern Cape
- Description: Effects of Acacia karroo supplementation on meat quality of Xhosa Lop-Eared goats The objective of the study was to determine the effects of A. karroo supplementation on the quality of meat from Xhosa lop-eared goats. Eighteen castrated 4-month-old Xhosa lop-eared male kids were kept at the University of Fort Hare Farm until slaughter. At the beginning of the experiment the goats had a mean body weight of 13.5 ± 0.31 kg (mean ± S.E.) and a mean body condition score (BCS) of 3.3 ± 0.16 (mean ± S.E.). From birth until weaning the kids were kept on natural pastures with their mothers. After weaning the goats were housed in an open sided barn for a period of 60 days, and were fed 500 g/head/day of Medicago sativa hay covering their maintenance and growth needs. For the purpose of the experiment, the goats were randomly divided into two balanced treatment groups of nine goats each, supplemented group (AK) and non-supplemented group (NS). The supplemented group received an additional 200g per head per day of fresh A. karroo leaves collected each day for two months. Supplementary feed was given to the goats individually in feeding troughs. The kids were slaughtered at 60 days old and samples for meat quality assessment were taken from the Longistimus dorsi muscle. The effect of A. karroo supplementation on meat quality measurements such as ultimate pH, colour and cooking losses of meat from indigenous Xhosa lop-eared goats were determined. The effect of A. karroo supplementation on the consumer sensory characteristic scores of the meat from the indigenous Xhosa lop-eared breed was also determined. There was a significant effect of the A. karroo supplementation on the meat quality measurements except for L* and a* colour coordinates. Meat from the A. karroo supplemented goats had lower pH and cooking loss scores than that of the non-supplemented goats. Acacia karroo supplementation improves meat tenderness and juiciness. There were no significant effects of A. karroo supplementation on the flavour and off- iv flavours of the meat from the supplemented goats. However, thermal preparation and consumer background had a significant effect on the meat sensory characteristics. The cooked meat had significantly higher sensory scores than the roasted meat. Female consumers reported higher sensory scores than male consumers. Consumers of different tribes and ages also reported significantly different sensory scores of meat from indigenous Xhosa lop-eared goats. Therefore, this study indicated that A. karroo supplementation can be fed to the Xhosa lop-eared goats to improve their meat quality.
- Full Text:
- Date Issued: 2011
- Authors: Ngambu, Simthembile
- Date: 2011
- Subjects: Goats -- Nutrition -- South Africa , Food -- Sensory evaluation , Goat meat -- South Africa , Goats -- Feeding and feeds -- South Africa , Meat -- Quality -- South Africa , Goats -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11808 , http://hdl.handle.net/10353/450 , Goats -- Nutrition -- South Africa , Food -- Sensory evaluation , Goat meat -- South Africa , Goats -- Feeding and feeds -- South Africa , Meat -- Quality -- South Africa , Goats -- South Africa -- Eastern Cape
- Description: Effects of Acacia karroo supplementation on meat quality of Xhosa Lop-Eared goats The objective of the study was to determine the effects of A. karroo supplementation on the quality of meat from Xhosa lop-eared goats. Eighteen castrated 4-month-old Xhosa lop-eared male kids were kept at the University of Fort Hare Farm until slaughter. At the beginning of the experiment the goats had a mean body weight of 13.5 ± 0.31 kg (mean ± S.E.) and a mean body condition score (BCS) of 3.3 ± 0.16 (mean ± S.E.). From birth until weaning the kids were kept on natural pastures with their mothers. After weaning the goats were housed in an open sided barn for a period of 60 days, and were fed 500 g/head/day of Medicago sativa hay covering their maintenance and growth needs. For the purpose of the experiment, the goats were randomly divided into two balanced treatment groups of nine goats each, supplemented group (AK) and non-supplemented group (NS). The supplemented group received an additional 200g per head per day of fresh A. karroo leaves collected each day for two months. Supplementary feed was given to the goats individually in feeding troughs. The kids were slaughtered at 60 days old and samples for meat quality assessment were taken from the Longistimus dorsi muscle. The effect of A. karroo supplementation on meat quality measurements such as ultimate pH, colour and cooking losses of meat from indigenous Xhosa lop-eared goats were determined. The effect of A. karroo supplementation on the consumer sensory characteristic scores of the meat from the indigenous Xhosa lop-eared breed was also determined. There was a significant effect of the A. karroo supplementation on the meat quality measurements except for L* and a* colour coordinates. Meat from the A. karroo supplemented goats had lower pH and cooking loss scores than that of the non-supplemented goats. Acacia karroo supplementation improves meat tenderness and juiciness. There were no significant effects of A. karroo supplementation on the flavour and off- iv flavours of the meat from the supplemented goats. However, thermal preparation and consumer background had a significant effect on the meat sensory characteristics. The cooked meat had significantly higher sensory scores than the roasted meat. Female consumers reported higher sensory scores than male consumers. Consumers of different tribes and ages also reported significantly different sensory scores of meat from indigenous Xhosa lop-eared goats. Therefore, this study indicated that A. karroo supplementation can be fed to the Xhosa lop-eared goats to improve their meat quality.
- Full Text:
- Date Issued: 2011
Effects of colours, shapes and icons on performance and familiarity
- Authors: Dambuza, Inga Yola
- Date: 2011
- Subjects: Industrial hygiene -- Research -- South Africa , Industrial safety -- Research -- South Africa , Industrial hygiene -- Standards -- South Africa , Human engineering -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5117 , http://hdl.handle.net/10962/d1005195 , Industrial hygiene -- Research -- South Africa , Industrial safety -- Research -- South Africa , Industrial hygiene -- Standards -- South Africa , Human engineering -- Research -- South Africa
- Description: Occupational injuries and illnesses remain to be a heavy burden on workers and employees in industrial developing and industrially developed societies, and health and safety in workplaces continues to be an important issue for ergonomists. Steps are being taken to stimulate health and safety agendas and to discover ways in which health and safety in industries can be improved. The main responsibility of employers is to provide employees with information, instructions and training that they required to carry out their work tasks in a healthy, practical and safe manner. The role of education as a countermeasure to occupational injury and illness is being re-examined by health and safety practitioners and safety training is being considered as a vital part of accident prevention strategies. Effective training programmes should guarantee that workers possess the skills they require to complete their tasks in a safe and healthy manner. Very little is known about the type and quality of training workers undergo and how that training affects the safety outcomes of companies. There has been an attempt over the past 20 years to increase the research on safety communications and a great deal of this research has been focused on safety warnings; with the greatest attention been placed on the components of safety signs, such as colours, size, shapes and icons. The effects of these components on comprehension with relation to age and education have not received the same amount of attention. The impact of familiarity on safety warnings with respect to age and education has also received very little attention; despite the knowledge that familiarity has been shown to increase the noticing of warnings and the comprehension of safety information. Despite the increase in the research on safety communication, the literature and research in South Africa is scarce. Studies present in South Africa do not encompass the comprehension of safety signs or the ability of individuals with different age and education levels to learn the information included in the signs. Due to the multi-linguistic nature of South Africa and the fact that South Africa is an Industrially Developing Country (IDC) with high levels of illiteracy, issues such as the comprehension of safety information must be addressed. Therefore, the objectives of this study were to investigate the effects of safety sign attributes on learning and familiarity, in subjects that differed in age and education levels. These effects were investigated through measuring the reaction and response times of the different subject groups, as well as the number of components in the safety signs that were recalled correctly. The combined results of these responses were used as a measure for familiarity. A set of signs was designed for the study by the researcher using three different colours, three different shapes, three different icons and text. Certain variables were omitted from some signs to create the test pool and the eight conditions that were tested in a laboratory setting. Each condition contained different components of the designed signs and 60 subjects were used to test these conditions. The subjects were placed in groups according to their age and level of education. Subjects were required to learn a set of 64 signs, either “With Occlusion” or “Without Occlusion”, and asked to recall the meanings of the components in the signs. Reaction time, response time and error rate were measured from the responses. The results showed that the conditions resulted in different reaction times, response times and error rates for all subjects. The signs containing a combination of shapes and text resulted in the best performance. Age and education were found to have a significant effect on various performance criteria as did the method in which the signs were displayed (Occlusion and No Occlusion). The increased repetitions and sessions elicited lower reaction times, response times and error rates. The conclusions drawn from this study suggest that different attributes be considered carefully when subjects are expected to learn and recall information in safety signs. The results also highlighted the need to increase the exposure of individuals to safety signs in order to increase familiarity and ultimately improve the recall and comprehension of the attributes.
- Full Text:
- Date Issued: 2011
- Authors: Dambuza, Inga Yola
- Date: 2011
- Subjects: Industrial hygiene -- Research -- South Africa , Industrial safety -- Research -- South Africa , Industrial hygiene -- Standards -- South Africa , Human engineering -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5117 , http://hdl.handle.net/10962/d1005195 , Industrial hygiene -- Research -- South Africa , Industrial safety -- Research -- South Africa , Industrial hygiene -- Standards -- South Africa , Human engineering -- Research -- South Africa
- Description: Occupational injuries and illnesses remain to be a heavy burden on workers and employees in industrial developing and industrially developed societies, and health and safety in workplaces continues to be an important issue for ergonomists. Steps are being taken to stimulate health and safety agendas and to discover ways in which health and safety in industries can be improved. The main responsibility of employers is to provide employees with information, instructions and training that they required to carry out their work tasks in a healthy, practical and safe manner. The role of education as a countermeasure to occupational injury and illness is being re-examined by health and safety practitioners and safety training is being considered as a vital part of accident prevention strategies. Effective training programmes should guarantee that workers possess the skills they require to complete their tasks in a safe and healthy manner. Very little is known about the type and quality of training workers undergo and how that training affects the safety outcomes of companies. There has been an attempt over the past 20 years to increase the research on safety communications and a great deal of this research has been focused on safety warnings; with the greatest attention been placed on the components of safety signs, such as colours, size, shapes and icons. The effects of these components on comprehension with relation to age and education have not received the same amount of attention. The impact of familiarity on safety warnings with respect to age and education has also received very little attention; despite the knowledge that familiarity has been shown to increase the noticing of warnings and the comprehension of safety information. Despite the increase in the research on safety communication, the literature and research in South Africa is scarce. Studies present in South Africa do not encompass the comprehension of safety signs or the ability of individuals with different age and education levels to learn the information included in the signs. Due to the multi-linguistic nature of South Africa and the fact that South Africa is an Industrially Developing Country (IDC) with high levels of illiteracy, issues such as the comprehension of safety information must be addressed. Therefore, the objectives of this study were to investigate the effects of safety sign attributes on learning and familiarity, in subjects that differed in age and education levels. These effects were investigated through measuring the reaction and response times of the different subject groups, as well as the number of components in the safety signs that were recalled correctly. The combined results of these responses were used as a measure for familiarity. A set of signs was designed for the study by the researcher using three different colours, three different shapes, three different icons and text. Certain variables were omitted from some signs to create the test pool and the eight conditions that were tested in a laboratory setting. Each condition contained different components of the designed signs and 60 subjects were used to test these conditions. The subjects were placed in groups according to their age and level of education. Subjects were required to learn a set of 64 signs, either “With Occlusion” or “Without Occlusion”, and asked to recall the meanings of the components in the signs. Reaction time, response time and error rate were measured from the responses. The results showed that the conditions resulted in different reaction times, response times and error rates for all subjects. The signs containing a combination of shapes and text resulted in the best performance. Age and education were found to have a significant effect on various performance criteria as did the method in which the signs were displayed (Occlusion and No Occlusion). The increased repetitions and sessions elicited lower reaction times, response times and error rates. The conclusions drawn from this study suggest that different attributes be considered carefully when subjects are expected to learn and recall information in safety signs. The results also highlighted the need to increase the exposure of individuals to safety signs in order to increase familiarity and ultimately improve the recall and comprehension of the attributes.
- Full Text:
- Date Issued: 2011
Effects of genetically modified maize (MON810) and its residues on the functional diversity of microorganisms in two South African soils
- Authors: Puta, Usanda
- Date: 2011
- Subjects: Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11250 , http://hdl.handle.net/10353/419 , Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Description: Genetically modified (GM) crops are commercially cultivated worldwide but there are concerns on their possible negative impacts on soil biodiversity. A glasshouse study was conducted to determine effects of Bt maize residues on soil microbial diversity. Residues of Bt maize (PAN 6Q-308B) and non-Bt maize (PAN 6Q-121) were incorporated into the soil and corresponding maize seeds planted. The treatments were replicated three times. Fertilizer and water application were similar for both treatments. Rhizosphere and bulk soil was destructively sampled from each treatment and analyzed for microbial community level physiological profiles using Biolog plates with 31 different carbon substrates. Absorbance in the Biolog plates was recorded after 72 h of incubation at 20oC. Arbuscular mycorrhizal fungi spore counts were also determined. Field studies were conducted at the University of Free State and University of Fort Hare Research Farms to determine the effects of growing Bt maize on soil microbial diversity. One Bt maize cultivar (PAN6Q-308B) and non-Bt maize (PAN6Q-121) were grown in a paired experiment at University of Free State farm, while two Bt maize (DKC61-25B and PAN6Q-321B) and their near-isogenic non-Bt maize lines (DKC61-24 and PAN6777) were grown in a randomized complete block design with three replicates. Fertilization, weed control and water application, were similar for both Bt maize cultivars and their non-Bt maize counterparts. Rhizosphere soil samples were collected by uprooting whole plants and collecting the soil attached to the roots. The samples were analysed for microbial diversity and for arbuscular mycorrhizae fungal spore counts. Principal component analysis showed that soil microbial diversity was affected more by sampling time whereas genetic modification had minimal effects. Presence of residues also increased the diversity of microorganisms. Mycorrhizal fungal spores were not affected by the presence of Bt maize residues. Growing Bt maize had no effect on the soil microbial diversity in the rhizosphere.
- Full Text:
- Date Issued: 2011
- Authors: Puta, Usanda
- Date: 2011
- Subjects: Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11250 , http://hdl.handle.net/10353/419 , Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Description: Genetically modified (GM) crops are commercially cultivated worldwide but there are concerns on their possible negative impacts on soil biodiversity. A glasshouse study was conducted to determine effects of Bt maize residues on soil microbial diversity. Residues of Bt maize (PAN 6Q-308B) and non-Bt maize (PAN 6Q-121) were incorporated into the soil and corresponding maize seeds planted. The treatments were replicated three times. Fertilizer and water application were similar for both treatments. Rhizosphere and bulk soil was destructively sampled from each treatment and analyzed for microbial community level physiological profiles using Biolog plates with 31 different carbon substrates. Absorbance in the Biolog plates was recorded after 72 h of incubation at 20oC. Arbuscular mycorrhizal fungi spore counts were also determined. Field studies were conducted at the University of Free State and University of Fort Hare Research Farms to determine the effects of growing Bt maize on soil microbial diversity. One Bt maize cultivar (PAN6Q-308B) and non-Bt maize (PAN6Q-121) were grown in a paired experiment at University of Free State farm, while two Bt maize (DKC61-25B and PAN6Q-321B) and their near-isogenic non-Bt maize lines (DKC61-24 and PAN6777) were grown in a randomized complete block design with three replicates. Fertilization, weed control and water application, were similar for both Bt maize cultivars and their non-Bt maize counterparts. Rhizosphere soil samples were collected by uprooting whole plants and collecting the soil attached to the roots. The samples were analysed for microbial diversity and for arbuscular mycorrhizae fungal spore counts. Principal component analysis showed that soil microbial diversity was affected more by sampling time whereas genetic modification had minimal effects. Presence of residues also increased the diversity of microorganisms. Mycorrhizal fungal spores were not affected by the presence of Bt maize residues. Growing Bt maize had no effect on the soil microbial diversity in the rhizosphere.
- Full Text:
- Date Issued: 2011
Effects of habitat patch size and isolation on the population structure of two siphonarian limpets
- Authors: Johnson, Linda Gail
- Date: 2011
- Subjects: Siphonaria , Limpets , Population biology , Marine ecology , Habitat selection , Animals -- Dispersal , Ecological heterogeneity , Animal populations , Biodiversity
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5679 , http://hdl.handle.net/10962/d1005364 , Siphonaria , Limpets , Population biology , Marine ecology , Habitat selection , Animals -- Dispersal , Ecological heterogeneity , Animal populations , Biodiversity
- Description: Habitat fragmentation is a fundamental process that determines trends and patterns of distribution and density of organisms. These patterns and trends have been the focus of numerous terrestrial and marine studies and have led to the development of several explanatory hypotheses. Systems and organisms are dynamic and no single hypothesis has adequately accounted for these observed trends. It is therefore important to understand the interaction of these processes and patterns to explain the mechanisms controlling population dynamics. The main aim of this thesis was to test the effect of patch size and isolation on organisms with different modes of dispersal. Mode of dispersal has previously been examined as a factor influencing the effects that habitat fragmentation has on organisms. Very few studies have, however, examined the mode of dispersal of marine organisms because it has long been assumed that marine animals are not directly influenced by habitat fragmentation because of large-scale dispersal. I used two co-occurring species of siphonariid limpets with different modes of dispersal to highlight that not only are marine organisms affected by habitat fragmentation but that they are affected in different ways. The two species of limpet, Siphonaria serrata and Siphonaria concinna, are found within the same habitat and have the same geographic range along the South African coastline, however, they have different modes of dispersal and development. The effect of patch size on organism density has been examined to a great extent with varied results. This study investigated whether habitat patch size played a key role in determining population density and limpet body sizes. The two species are found on the eastern and southern coasts of South Africa were examined across this entire biogeographic range. Patch size was found to have a significant effect on population density of the pelagic developer, S. concinna, but not the direct developing S. serrata. Patch size did play a role in determining limpet body size for both species. S. concinna body size was proposed to be effected directly by patch size whilst S. serrata body size was proposed to be affected indirectly by the effects of the S. concinna densities. The same patterns and trends were observed at five of the seven examined regions across the biogeographic range. The trends observed for S. concinna with respect to patch size conform to the source-sink hypothesis with large habitat patches acting as the source populations whilst the small habitat patches acted as the sink populations. Many previous studies have focused on the effects of habitat patch size at one point in time or over one season. I tested the influence of habitat patch size on the two species of limpets over a period of twelve months to determine whether the trends observed were consistent over time or whether populations varied with time. S. concinna showed a consistently significant difference between small and large patches; whilst S. serrata did not follow a consistent pattern. The mode of dispersal for the two limpets was used to explain the different trends shown by the two species. This examination allowed for the determining of source and sink populations for S. concinna through the examination of fluctuations in limpet body sizes and population densities at small and large habitat patches over twelve months. The direct developing S. serrata trends could not be explained using source-sink theory, as populations were independent from one another. S. serrata demonstrated body size differences at small and large patches which, may be explained by interspecific and intraspecific competition. Habitat isolation is known to play an important role in determining the structure of assemblages and the densities of populations. In this study the population density of the pelagic developing S. concinna showed a weak influence of degree of isolation whilst that of the direct developing S. serrata did not, which may be because of habitat patches along the South African coastline not having great enough degrees of isolation. The population size-structure was influenced directly influenced by isolation for S. concinna, whilst the different population size structure for S. serrata may be explained by assemblage co-dependence. The mode of dispersal showed effects on the relationship of population density and population size-structure with habitat size and isolation. This study indicates the importance of investigating patterns and processes across a range of spatial and temporal scales to gain a comprehensive understanding of factors effecting intertidal organisms.
- Full Text:
- Date Issued: 2011
- Authors: Johnson, Linda Gail
- Date: 2011
- Subjects: Siphonaria , Limpets , Population biology , Marine ecology , Habitat selection , Animals -- Dispersal , Ecological heterogeneity , Animal populations , Biodiversity
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5679 , http://hdl.handle.net/10962/d1005364 , Siphonaria , Limpets , Population biology , Marine ecology , Habitat selection , Animals -- Dispersal , Ecological heterogeneity , Animal populations , Biodiversity
- Description: Habitat fragmentation is a fundamental process that determines trends and patterns of distribution and density of organisms. These patterns and trends have been the focus of numerous terrestrial and marine studies and have led to the development of several explanatory hypotheses. Systems and organisms are dynamic and no single hypothesis has adequately accounted for these observed trends. It is therefore important to understand the interaction of these processes and patterns to explain the mechanisms controlling population dynamics. The main aim of this thesis was to test the effect of patch size and isolation on organisms with different modes of dispersal. Mode of dispersal has previously been examined as a factor influencing the effects that habitat fragmentation has on organisms. Very few studies have, however, examined the mode of dispersal of marine organisms because it has long been assumed that marine animals are not directly influenced by habitat fragmentation because of large-scale dispersal. I used two co-occurring species of siphonariid limpets with different modes of dispersal to highlight that not only are marine organisms affected by habitat fragmentation but that they are affected in different ways. The two species of limpet, Siphonaria serrata and Siphonaria concinna, are found within the same habitat and have the same geographic range along the South African coastline, however, they have different modes of dispersal and development. The effect of patch size on organism density has been examined to a great extent with varied results. This study investigated whether habitat patch size played a key role in determining population density and limpet body sizes. The two species are found on the eastern and southern coasts of South Africa were examined across this entire biogeographic range. Patch size was found to have a significant effect on population density of the pelagic developer, S. concinna, but not the direct developing S. serrata. Patch size did play a role in determining limpet body size for both species. S. concinna body size was proposed to be effected directly by patch size whilst S. serrata body size was proposed to be affected indirectly by the effects of the S. concinna densities. The same patterns and trends were observed at five of the seven examined regions across the biogeographic range. The trends observed for S. concinna with respect to patch size conform to the source-sink hypothesis with large habitat patches acting as the source populations whilst the small habitat patches acted as the sink populations. Many previous studies have focused on the effects of habitat patch size at one point in time or over one season. I tested the influence of habitat patch size on the two species of limpets over a period of twelve months to determine whether the trends observed were consistent over time or whether populations varied with time. S. concinna showed a consistently significant difference between small and large patches; whilst S. serrata did not follow a consistent pattern. The mode of dispersal for the two limpets was used to explain the different trends shown by the two species. This examination allowed for the determining of source and sink populations for S. concinna through the examination of fluctuations in limpet body sizes and population densities at small and large habitat patches over twelve months. The direct developing S. serrata trends could not be explained using source-sink theory, as populations were independent from one another. S. serrata demonstrated body size differences at small and large patches which, may be explained by interspecific and intraspecific competition. Habitat isolation is known to play an important role in determining the structure of assemblages and the densities of populations. In this study the population density of the pelagic developing S. concinna showed a weak influence of degree of isolation whilst that of the direct developing S. serrata did not, which may be because of habitat patches along the South African coastline not having great enough degrees of isolation. The population size-structure was influenced directly influenced by isolation for S. concinna, whilst the different population size structure for S. serrata may be explained by assemblage co-dependence. The mode of dispersal showed effects on the relationship of population density and population size-structure with habitat size and isolation. This study indicates the importance of investigating patterns and processes across a range of spatial and temporal scales to gain a comprehensive understanding of factors effecting intertidal organisms.
- Full Text:
- Date Issued: 2011
Effects of polarization in a distributed raman fibre amplifier
- Authors: Muguro, Kennedy Mwaura
- Date: 2011
- Subjects: Fiber optics , Polarization (Light) , Optical communications , Optical amplifiers , Raman effect
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10544 , http://hdl.handle.net/10948/d1014621
- Description: The need to exploit the large fibre bandwidth and increase the reach has seen the application of the Raman fibre amplifier (RFA) become indispensable in modern light wave systems. The success and resilience of RFAs in optical communication is deeply rooted in their unique optical properties and new technologies which have allowed the amplifier to come of age. However, the full potential of RFAs in optical communication and other applications are yet to be realized. More so are its polarization properties which still remain largely unexploited and have not been fully understood. In this work, fundamental issues regarding distributed RFA have been investigated with the aim of acquiring a better understanding of the amplifier polarization characteristics which have potential applications. In particular the effects of polarization mode dispersion (PMD) and polarization dependent loss (PDL) have been demonstrated both by simulation and experiment. The possibility of Raman polarization pulling in single mode fibres (SMFs) has also been addressed. Polarization sensitivity of RFA has been known for a long time but the clear manifestation of it has become evident in the advent of modern low PMD fibre. Unlike EDFAs which make use of special doped fibre, RFAs require no special fibre for operation. Besides, RFA uses a very long length of fibre and as such the fibre polarization characteristics come into play during amplification. In the demonstrations presented in this thesis a fibre of PMD coefficient < 0.05 pskm-1/2 was regarded as low PMD fibre while one having coefficient ≥ 0.05 pskm-1/2 was categorized to have high PMD unless otherwise stated. Several experiments were performed to evaluate the RFA gain characteristics with respect to fibre PMD and the system performance in the presence of noise emanating from amplified spontaneous emission (ASE). Analysis of Raman gain statistics was done for fibres of low and high PMD coefficients. The statistics of PDG and on-off gain were eventually used to demonstrate the extraction of PMD coefficients of fibres between 0.01- 0.1 pskm-1/2 using a forward pumping configuration. It was found that, at increasing pump power a linear relationship exists between forward and backward signal gain on a dB scale. The interaction of PDL and Raman PDG in the presence of PMD were observed at very fundamental level. It was found the presence of PDL serves to reduce the available on-off gain. It was also established that the presence of PMD mediates the interaction between PDG/PDL. When PMD is high it reduces PDG but the presence of PDL introduces a wavelength dependent gain tilting for WDM channels. Further analysis revealed that signal polarization is influenced by the pump SOP due to the pulling effect which is present even at moderate pump power.
- Full Text:
- Date Issued: 2011
- Authors: Muguro, Kennedy Mwaura
- Date: 2011
- Subjects: Fiber optics , Polarization (Light) , Optical communications , Optical amplifiers , Raman effect
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10544 , http://hdl.handle.net/10948/d1014621
- Description: The need to exploit the large fibre bandwidth and increase the reach has seen the application of the Raman fibre amplifier (RFA) become indispensable in modern light wave systems. The success and resilience of RFAs in optical communication is deeply rooted in their unique optical properties and new technologies which have allowed the amplifier to come of age. However, the full potential of RFAs in optical communication and other applications are yet to be realized. More so are its polarization properties which still remain largely unexploited and have not been fully understood. In this work, fundamental issues regarding distributed RFA have been investigated with the aim of acquiring a better understanding of the amplifier polarization characteristics which have potential applications. In particular the effects of polarization mode dispersion (PMD) and polarization dependent loss (PDL) have been demonstrated both by simulation and experiment. The possibility of Raman polarization pulling in single mode fibres (SMFs) has also been addressed. Polarization sensitivity of RFA has been known for a long time but the clear manifestation of it has become evident in the advent of modern low PMD fibre. Unlike EDFAs which make use of special doped fibre, RFAs require no special fibre for operation. Besides, RFA uses a very long length of fibre and as such the fibre polarization characteristics come into play during amplification. In the demonstrations presented in this thesis a fibre of PMD coefficient < 0.05 pskm-1/2 was regarded as low PMD fibre while one having coefficient ≥ 0.05 pskm-1/2 was categorized to have high PMD unless otherwise stated. Several experiments were performed to evaluate the RFA gain characteristics with respect to fibre PMD and the system performance in the presence of noise emanating from amplified spontaneous emission (ASE). Analysis of Raman gain statistics was done for fibres of low and high PMD coefficients. The statistics of PDG and on-off gain were eventually used to demonstrate the extraction of PMD coefficients of fibres between 0.01- 0.1 pskm-1/2 using a forward pumping configuration. It was found that, at increasing pump power a linear relationship exists between forward and backward signal gain on a dB scale. The interaction of PDL and Raman PDG in the presence of PMD were observed at very fundamental level. It was found the presence of PDL serves to reduce the available on-off gain. It was also established that the presence of PMD mediates the interaction between PDG/PDL. When PMD is high it reduces PDG but the presence of PDL introduces a wavelength dependent gain tilting for WDM channels. Further analysis revealed that signal polarization is influenced by the pump SOP due to the pulling effect which is present even at moderate pump power.
- Full Text:
- Date Issued: 2011
Effects of pre-slaughter handling on pork quality from a smallholder abbatoir
- Authors: Gajana, Christian Sabelo
- Date: 2011
- Subjects: Meat cuts -- Standards , Pork -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11174 , http://hdl.handle.net/10353/434 , Meat cuts -- Standards , Pork -- Quality
- Description: Effects of pre-slaughter handling on pork quality from a smallholder abattoir By Christian Sabelo Gajana A total of 158 pork samples from Landrace and Large White pigs were collected from Nxuba Municipal abattoir to determine the effects of pre-slaughter handling on pork quality. Technological quality attributes of pork such as pHu, L*, a*, b*, WBSF values, TL%, CL% EL% were determined. Breed differences were observed in ultimate pH (pHu) and redness (a*) with meat from the Large White having higher pHu and lower a* values than meat from the Landrace. Meat from pigs slaughtered in spring season had the highest (P<0.05) shear force values (27.6±1.58) while the lowest shear force values (18.5±1.58) were found in meat from pigs that were slaughtered in the autumn season. The lowest pHu and highest a* values were found in meat from 9 month-old pigs (5.3±0.07). The 6, 9 and 10 months had highest (P<0.05) than 7 and 8 months old pigs. Cooking loss and evaporation loss increased with age groups. Only distance and transportation time had a positive relationship to TL% of pork whilst no significant relationship was reported between all the other pre-slaughter variables and technological quality attributes of pork. Highest risks of PSE occurrences were observed with more space allowance of 0.4 m2 per 100 kg during transportation. Reduced risks of PSE occurrences were observed with space allowance of 0.35 m2 per 100 kg. The pHu were positively correlated to WBSF values (P < 0.001). The L* values were positively correlated to b* (P < 0.001), and negatively correlated to a* while CL and EL were positively correlated to L*. In the current study, a* values were positively correlated to b* (P < 0.001) but negatively correlated to EL (P < 0.01). There were iv positive correlations between CL and EL, Both CL and EL were positively correlated to WBSF values. The first four principal components (PCs) explained about 95% of the total variability for technological quality attributes of pork. It can be concluded that pig breeds vary in pHu and considerations should be made on different seasons of slaughter when assessing pork quality. Precautions should also be made on age categories of animal when improving the quality of pork. Transportation time, distance, stocking density, temperature and lairage time did not show significant relationship with technological quality attributes measured; except for transportation time and distance travelled which had a positive relationship with TL%. The risk of PSE increased with more space availability more than 0.4 m2 per 100 kg pig, but stocking density of 0.35 m2 per 100 kg was an ideal reduce the risk of PSE pork.
- Full Text:
- Date Issued: 2011
- Authors: Gajana, Christian Sabelo
- Date: 2011
- Subjects: Meat cuts -- Standards , Pork -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11174 , http://hdl.handle.net/10353/434 , Meat cuts -- Standards , Pork -- Quality
- Description: Effects of pre-slaughter handling on pork quality from a smallholder abattoir By Christian Sabelo Gajana A total of 158 pork samples from Landrace and Large White pigs were collected from Nxuba Municipal abattoir to determine the effects of pre-slaughter handling on pork quality. Technological quality attributes of pork such as pHu, L*, a*, b*, WBSF values, TL%, CL% EL% were determined. Breed differences were observed in ultimate pH (pHu) and redness (a*) with meat from the Large White having higher pHu and lower a* values than meat from the Landrace. Meat from pigs slaughtered in spring season had the highest (P<0.05) shear force values (27.6±1.58) while the lowest shear force values (18.5±1.58) were found in meat from pigs that were slaughtered in the autumn season. The lowest pHu and highest a* values were found in meat from 9 month-old pigs (5.3±0.07). The 6, 9 and 10 months had highest (P<0.05) than 7 and 8 months old pigs. Cooking loss and evaporation loss increased with age groups. Only distance and transportation time had a positive relationship to TL% of pork whilst no significant relationship was reported between all the other pre-slaughter variables and technological quality attributes of pork. Highest risks of PSE occurrences were observed with more space allowance of 0.4 m2 per 100 kg during transportation. Reduced risks of PSE occurrences were observed with space allowance of 0.35 m2 per 100 kg. The pHu were positively correlated to WBSF values (P < 0.001). The L* values were positively correlated to b* (P < 0.001), and negatively correlated to a* while CL and EL were positively correlated to L*. In the current study, a* values were positively correlated to b* (P < 0.001) but negatively correlated to EL (P < 0.01). There were iv positive correlations between CL and EL, Both CL and EL were positively correlated to WBSF values. The first four principal components (PCs) explained about 95% of the total variability for technological quality attributes of pork. It can be concluded that pig breeds vary in pHu and considerations should be made on different seasons of slaughter when assessing pork quality. Precautions should also be made on age categories of animal when improving the quality of pork. Transportation time, distance, stocking density, temperature and lairage time did not show significant relationship with technological quality attributes measured; except for transportation time and distance travelled which had a positive relationship with TL%. The risk of PSE increased with more space availability more than 0.4 m2 per 100 kg pig, but stocking density of 0.35 m2 per 100 kg was an ideal reduce the risk of PSE pork.
- Full Text:
- Date Issued: 2011
Effects of selected modes of digital distribution on music consumerism, with reference to the album format
- Authors: Du Preez, Liska
- Date: 2011
- Subjects: Sound -- Recording and reproducing -- Digital techniques , Music -- Technological innovations , Music and the Internet
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:8504 , http://hdl.handle.net/10948/1427 , Sound -- Recording and reproducing -- Digital techniques , Music -- Technological innovations , Music and the Internet
- Description: In this digital age many listeners of music now purchase albums from online digital music stores instead of buying a physical album from the record store. This has created a concern with many regarding the future of the album as a physical medium. This study investigates the impact of the possible death of the album on certain listening habits, the activity of record collecting, the creation of large-scale musical works, music consumerism, and its implications for the creative process on music as art and sound-recording quality. Three realisations have led to the problem statement. Firstly, the album might not have a future in the digital age. Secondly, downloadable songs might not be able to recreate an album experience. The third realisation is that the possible death of the album could create new, exciting challenges to artists as they strive to create art. This study is exploratory in nature - and no hypothesis was generated. The research necessitates qualitative, exploratory, descriptive and contextual approaches. Furthermore, the investigation has led to the collection of mostly new data, constituting a “primary data design” through the implementation of qualitative listening experiments and a focus group, using full-time NMMU students between 18 and 25 years of age. It is herein argued that digital distribution might possibly have an effect on the perception of the album format and might possibly still be a relevant listening experience, valued by young people. Singles and albums are collectable; and good albums are considered artistic and comparable to the large-scale musical works of the past. If the concept of an album does not die out, then high fidelity formats should be able to re-invent the album experience. Listening experiences other than the album experience do exist, and they could generate new ways for artists to create musical art.
- Full Text:
- Date Issued: 2011
- Authors: Du Preez, Liska
- Date: 2011
- Subjects: Sound -- Recording and reproducing -- Digital techniques , Music -- Technological innovations , Music and the Internet
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:8504 , http://hdl.handle.net/10948/1427 , Sound -- Recording and reproducing -- Digital techniques , Music -- Technological innovations , Music and the Internet
- Description: In this digital age many listeners of music now purchase albums from online digital music stores instead of buying a physical album from the record store. This has created a concern with many regarding the future of the album as a physical medium. This study investigates the impact of the possible death of the album on certain listening habits, the activity of record collecting, the creation of large-scale musical works, music consumerism, and its implications for the creative process on music as art and sound-recording quality. Three realisations have led to the problem statement. Firstly, the album might not have a future in the digital age. Secondly, downloadable songs might not be able to recreate an album experience. The third realisation is that the possible death of the album could create new, exciting challenges to artists as they strive to create art. This study is exploratory in nature - and no hypothesis was generated. The research necessitates qualitative, exploratory, descriptive and contextual approaches. Furthermore, the investigation has led to the collection of mostly new data, constituting a “primary data design” through the implementation of qualitative listening experiments and a focus group, using full-time NMMU students between 18 and 25 years of age. It is herein argued that digital distribution might possibly have an effect on the perception of the album format and might possibly still be a relevant listening experience, valued by young people. Singles and albums are collectable; and good albums are considered artistic and comparable to the large-scale musical works of the past. If the concept of an album does not die out, then high fidelity formats should be able to re-invent the album experience. Listening experiences other than the album experience do exist, and they could generate new ways for artists to create musical art.
- Full Text:
- Date Issued: 2011
Electrochemical behavior of phthalocyanines containing high oxidation state central metals
- Chauke, Vongani, Arslanoglu, Yasin, Nyokong, Tebello
- Authors: Chauke, Vongani , Arslanoglu, Yasin , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247111 , vital:51547 , xlink:href="https://doi.org/10.1016/j.poly.2011.05.028"
- Description: The syntheses of 2,(3)-(peripheral) and 1,(4)-(non-peripheral) (2-mercaptopyridine)phthalocyanine complexes of titanium(IV) oxide (5a and 6a, respectively), vanadium(IV) oxide (7a and 8a, respectively) and tantalum(V) hydroxide (9a, peripheral only) and their electrochemical characterization are presented in this report. Their electrochemistry is compared to that of thiophenyl and thiobenzyl substituted derivatives. The non-peripherally substituted complexes are more difficult to reduce than peripherally substituted derivatives. In addition, the mercaptopyridine substituted derivatives are more difficult to reduce compared to benzylmercapto and phenylmercapto derivatives, and aryl easier reduce than alkyl substitution. Spectroelectrochemistry of the complexes confirmed metal and ring redox processes for TaPc and TiPc derivatives and ring based processes only for VPc complexes.
- Full Text:
- Date Issued: 2011
- Authors: Chauke, Vongani , Arslanoglu, Yasin , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247111 , vital:51547 , xlink:href="https://doi.org/10.1016/j.poly.2011.05.028"
- Description: The syntheses of 2,(3)-(peripheral) and 1,(4)-(non-peripheral) (2-mercaptopyridine)phthalocyanine complexes of titanium(IV) oxide (5a and 6a, respectively), vanadium(IV) oxide (7a and 8a, respectively) and tantalum(V) hydroxide (9a, peripheral only) and their electrochemical characterization are presented in this report. Their electrochemistry is compared to that of thiophenyl and thiobenzyl substituted derivatives. The non-peripherally substituted complexes are more difficult to reduce than peripherally substituted derivatives. In addition, the mercaptopyridine substituted derivatives are more difficult to reduce compared to benzylmercapto and phenylmercapto derivatives, and aryl easier reduce than alkyl substitution. Spectroelectrochemistry of the complexes confirmed metal and ring redox processes for TaPc and TiPc derivatives and ring based processes only for VPc complexes.
- Full Text:
- Date Issued: 2011
Electrochemical, microscopic and spectroscopic characterization of benzene diamine functionalized single walled carbon nanotube-cobalt (II) tetracarboxy-phthalocyanine conjugates
- Mugadza, Tawanda, Nyokong, Tebello
- Authors: Mugadza, Tawanda , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247859 , vital:51624 , xlink:href="https://doi.org/10.1016/j.jcis.2010.10.057"
- Description: In this paper we report on the synthesis and characterization of 1,4-benzene diamine (BDA) functionalized single walled carbon nanotubes linked to cobalt (II) tetracarboxy-phthalocyanine. The characterization of the conjugate was through UV–vis, FTIR and X-ray diffraction (XRD) spectroscopies and by transmission electron microscope (TEM) and electrochemical methods. The conjugate is used for the electrochemical characterization of diuron. The catalytic rate constant for diuron was 4.4 × 103 M−1 s−1 and the apparent electron transfer rate constant was 18.5 × 10−6 cm s−1. The linear dynamic range was 1.0 × 10−5–2.0 × 10−4 M, with a sensitivity of ∼0.42 A mol−1L cm−2 and a limit of detection of 0.18 μM using the 3δ notation.
- Full Text:
- Date Issued: 2011
- Authors: Mugadza, Tawanda , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247859 , vital:51624 , xlink:href="https://doi.org/10.1016/j.jcis.2010.10.057"
- Description: In this paper we report on the synthesis and characterization of 1,4-benzene diamine (BDA) functionalized single walled carbon nanotubes linked to cobalt (II) tetracarboxy-phthalocyanine. The characterization of the conjugate was through UV–vis, FTIR and X-ray diffraction (XRD) spectroscopies and by transmission electron microscope (TEM) and electrochemical methods. The conjugate is used for the electrochemical characterization of diuron. The catalytic rate constant for diuron was 4.4 × 103 M−1 s−1 and the apparent electron transfer rate constant was 18.5 × 10−6 cm s−1. The linear dynamic range was 1.0 × 10−5–2.0 × 10−4 M, with a sensitivity of ∼0.42 A mol−1L cm−2 and a limit of detection of 0.18 μM using the 3δ notation.
- Full Text:
- Date Issued: 2011
Electrooxidation of Chlorophenols Catalyzed by Nickel Octadecylphthalocyanine Adsorbed on Single‐Walled Carbon Nanotubes
- Khene, Samson, Nyokong, Tebello
- Authors: Khene, Samson , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247198 , vital:51555 , xlink:href="https://doi.org/10.1002/elan.201100155"
- Description: We described the synthesis of nickel octadecylphthalocyanine (NiPc(C10H21)8), followed by its adsorption on single-walled carbon nanotubes (SWCNT) to form SWCNT-NiPc(C10H21)8 conjugates. SWCNT-NiPc(C10H21)8 was used to modify a glassy carbon electrode (GCE) and for the electrooxidation of 4-chlorophenol and 2,4-dichlorophenol. The SWCNT and NiPc(C10H21)8 have a synergistic effect on each other in terms of improving electrocatalysis for the detection of chlorophenols. The stability of the electrode improved in the presence of NiPc(C10H21)8 or NiPc compared to the bare GCE. The presence of SWCNT improves the electrocatalytic behaviour of NiPc(C10H21)8 but not of unsubstituted NiPc. All modified electrodes showed improved stability towards the detection of 2,4-dichlorophenol. The best stability for 4-CP detection was observed in the presence of SWCNT for NiPc(C10H21)8.
- Full Text:
- Date Issued: 2011
- Authors: Khene, Samson , Nyokong, Tebello
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/247198 , vital:51555 , xlink:href="https://doi.org/10.1002/elan.201100155"
- Description: We described the synthesis of nickel octadecylphthalocyanine (NiPc(C10H21)8), followed by its adsorption on single-walled carbon nanotubes (SWCNT) to form SWCNT-NiPc(C10H21)8 conjugates. SWCNT-NiPc(C10H21)8 was used to modify a glassy carbon electrode (GCE) and for the electrooxidation of 4-chlorophenol and 2,4-dichlorophenol. The SWCNT and NiPc(C10H21)8 have a synergistic effect on each other in terms of improving electrocatalysis for the detection of chlorophenols. The stability of the electrode improved in the presence of NiPc(C10H21)8 or NiPc compared to the bare GCE. The presence of SWCNT improves the electrocatalytic behaviour of NiPc(C10H21)8 but not of unsubstituted NiPc. All modified electrodes showed improved stability towards the detection of 2,4-dichlorophenol. The best stability for 4-CP detection was observed in the presence of SWCNT for NiPc(C10H21)8.
- Full Text:
- Date Issued: 2011