Heterodimer formation by Oct4 and Smad3 differentially regulates epithelial-to-mesenchymal transition-associated factors in breast cancer progression:
- Authors: Mandal, Gunjan , Biswas, Subir , Chowdhury, Sougata R , Chatterjee, Annesha , Purohit, Suman , Khamaru, Poulomi , Chakraborty, Sayan , Mandal, Palash K , Gupta, Arnab , de la Mare, Jo-Anne , Edkins, Adrienne L , Bhattacharyya, Arindam
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164907 , vital:41183 , DOI: 10.1016/j.bbadis.2018.03.010
- Description: The multifunctional cytokine TGF-β crucially participates in breast cancer (BCa) metastasis and works differently in the disease stages, thus contributing in BCa progression. We address connections between TGF-β and the stem cell-related transcription factor (TF) Oct4 in BCa. In 147 BCa patients with infiltrating duct carcinoma, we identified a significantly higher number of cases with both moderate/high Oct4 expression and high TGF-β in late stages compared to early stages of the disease.
- Full Text:
- Date Issued: 2018
In vitro analysis of putative cancer stem cell populations and chemosensitivity in the SW480 and SW620 colon cancer metastasis model:
- Authors: Slater, Cindy , de la Mare, Jo-Anne , Edkins, Adrienne L
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164401 , vital:41115 , DOI: 10.3892/ol.2018.8431
- Description: The cancer stem cell (CSC) theory implicates a small subpopulation of cells with stem like properties, which is responsible for tumour initiation, development and metastasis. The unique biological and functional characteristics of CSCs, widely associated with treatment resistance, indicate an association between metastasis and stemness. It was hypothesised that metastatic cell lines may be enriched in CSCs and that this would correlate with a more resistant tumour. In the present study, the SW480 and SW620 paired cell lines derived from a colon adenocarcinoma and its lymph node metastasis, respectively were compared as an in vitro model of cancer progression. Their chemosensitivity and CSC properties were investigated. A range of in vitro assays were performed, including the side population assay, ALDEFLUOR assay, tumoursphere assay and assessment of CSC associated surface phenotypes.
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- Date Issued: 2018