Out of place: an analysis of place as a contributor to the complexities of my “coloured” identity in Malabar, Gqeberha
- Authors: Flowers, Victoria
- Date: 2024-04
- Subjects: Colored people (South Africa) -- Race identity , Ethnicity in art -- South Africa -- Port Elizabeth , Modernism (Art)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62780 , vital:72954
- Description: Coloured identity has been a historical point of contestation in South Africa because Colouredness is assumed as “an inbred quality that arises automatically from miscegenation while instrumentalists share the essentialist premise that coloured identity is something negative and undesirable but blame it on the racism and the exploitative practices of the ruling white minority” (Adhikari, 2009: 15). This archaic belief attributed to racial mixture has now become redundant, due to the emergence of new hybridised Coloured identities that factor other ethnic and cultural groups as well as internal and external elements into their conception. The role of Place in the formation of Coloured identities is at the core of this fine arts based enquiry. This study is located in Malabar, Gqeberha which was established after the forced removals onset by the Group Areas Act of 1950 and the displacement of residents from South End. Additionally, it considers specific locations significant in their linkages to hybridised peoples, and how the place and individuals have been influenced by historical legacies of colonisation and apartheid. These are examined to consider their function in contextualising contemporary lived experiences of Coloured identity within the community of Malabar and its effect on artistic practice through autoethnographic research. Through this autoethnographic and reflexive study and the accompanying empirical research process, I critically examine and explore my Coloured identity as part of being-in-the-world (Heidegger, 1962). This ‘being’ is multifaceted as a result of the significance of Place in South Africa and how it has shaped my perception of how and where I have a sense of being and belonging. , Thesis (MA) -- Faculty of Humanities, School Visual and Performing Arts, 2023
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- Date Issued: 2024-04
The complexities of transfer pricing methods and the role of advance pricing agreements and tax audits in addressing disputes
- Authors: Ndou, Wavhudi
- Date: 2023-10-13
- Subjects: Transfer pricing Taxation Law and legislation South Africa , Advance pricing agreement , Double taxation , Arms-length transactions , Tax auditing , Advance tax ruling , Organisation for Economic Co-operation and Development , United Nations , World Bank
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419631 , vital:71661
- Description: Base erosion and profit shifting is defined as the use of tax planning strategies by multinational enterprises, often through exploiting gaps and mismatches between the countries in which they operate (OECD, 2021: p. 1). Multinational enterprises exploit these gaps through the use of transfer pricing. Goods and services are exchanged between connected persons or associated enterprises at prices that do not reflect their arm’s length price, in order to shift profits from high tax to low tax jurisdictions. In terms of section 31 of the Income Tax Act, transactions between connected persons or associated enterprises must be reflected at their arm’s length price. Transfer pricing has become an issue due to the difficulties in determining an appropriate arm’s length price. Disputes arise between a taxpayer and a tax administration on the methods to use to determine an appropriate transfer price. The use of Advance Pricing Agreements prevents these disputes from arising and provides tax certainty on the treatment of transactions for both the taxpayer and the tax administration. While the OECD recommends the use of Advance Pricing Agreements as a method to prevent disputes from arising, the OECD also argued that if a country has the resources to conduct an audit, an Advance Pricing Agreement will not lead to increased revenue collection. The research therefore analyses the problems faced in determining an appropriate arm's length price and compares the role that Advance Pricing Agreements and audits play in addressing transfer pricing issues. The possible role of Advance Tax Rulings is also explored, but they are found not to be suitable, except for the most simple transactions. The research applies a legal interpretative, doctrinal research methodology and a qualitative research method. The data comprised of relevant South African tax legislation, OECD Guidelines, the World Bank Handbook, and the UN Manual, together with the writings of acknowledged experts in the field. The study establishes that a proper functioning audit system is crucial to increasing revenue collection once a country implements an Advance Pricing Agreement. The research therefore recommends the adoption of Advance Pricing Agreements in South Africa as a dispute prevention measure. , Thesis (MCom) -- Faculty of Commerce, Accounting, 2023
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- Date Issued: 2023-10-13
The de novo biosynthesis of biotin is required for the optimal growth of Salmonella enterica serovar Typhimurium in the intracellular environment
- Authors: McLaughlin, Claire
- Date: 2021-10-29
- Subjects: Salmonella , Biotin , Biosynthesis , Salmonella typhimurium , Antibacterial agents , Anti-infective agents , Pathogenic bacteria , Salmonella food poisoning
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192097 , vital:45195
- Description: Salmonella enterica serovar Typhimurium (S. Typhimurium) is a foodborne pathogen infecting humans and animals, contributing to significant morbidity and mortality worldwide each year. The increase in antibiotic-resistant S. Typhimurium infections in recent years has highlighted the need for new antibacterial drugs and drug targets. S. Typhimurium can acquire biotin through de novo biosynthesis or via transport from its extracellular environment. The importance of the vitamin for bacterial survival, coupled with the absence of the biotin biosynthetic pathway in humans, makes the biotin biosynthetic enzymes attractive targets for drug discovery. The study's primary aim was to determine the relative importance of the biotin biosynthesis and transport pathways for the in vitro and ex vivo growth and survival of S. Typhimurium, with the goal of validating the pathways as valid targets for antimicrobial drug development. In order to achieve this aim, we generated S. Typhimurium mutant strains harbouring deletions in either the biotin biosynthetic gene, bioB, or putative high-affinity biotin transporter, yigM (ΔbioB and ΔyigM, respectively), as well as a double mutant in which the two mutations were combined (ΔbioB ΔyigM). Since the simultaneous disruption of biotin biosynthesis and transport in the double mutant may form a synthetic lethal combination, preventing further analysis of the strain, we also constructed a conditional mutant in which the promoter of the yigM gene was replaced by the arabinose-regulatable, PBAD promoter in the ΔbioB background (ΔbioB PBAD::yigM). Since the expression of the YigM in this strain is arabinose-regulatable, its role as a biotin transporter can be evaluated by altering the arabinose concentration in the growth media. Once the mutant strains were isolated and verified genetically, their growth and that of their genetically complemented counterparts were analysed in liquid and/or solid M9 minimal medium in the absence of biotin. Consistent with previous observations, the ΔbioB auxotrophic mutant's growth was severely compromised in minimal media in the absence of biotin. The growth of the strain could, however, be restored by supplementation with exogenous biotin or expression of the wild type bioB gene from an episomal plasmid. The ability of biotin to reverse the growth defect of the ΔbioB mutant strain was, however, dependent on the presence of a functional YigM, since biotin supplementation did not affect the growth of the ΔbioB ΔyigM double mutant strain. The introduction of a second copy of the yigM gene in the ΔbioB ΔyigM background, however, restored the growth of the strain in the presence, but not absence, of biotin. The dependence of the double mutant on both YigM and biotin for growth supports the idea that the protein functions as the sole or primary biotin transporter in S. Typhimurium, as it has recently been shown for E. coli (Ringsletter, 2010; Finkenwirth et al, 2013). The essentiality of YigM for biotin transport was subsequently verified by two independent means. Firstly, the growth of the ΔbioB PBAD::yigM promoter-replacement mutant was strictly dependent on the inclusion of arabinose in biotin-supplemented M9 minimal media supplemented, indicating that the expression of YigM from the PBAD promoter is essential for biotin transport. Secondly, following treatment with a known small-molecule inhibitor of the biotin biosynthesis, MAC-13772, exogenous biotin was capable of restoring the growth defect of the YigM+ wild type S. Typhimurium strain, but not the YigM− ΔyigM mutant. Taken together, these findings confirm that YigM serves as the biotin transporter for S. Typhimurium and that the corresponding ΔyigM mutant is, as a result, defective for biotin transport. Having confirmed the genotypes and phenotypes of the ΔbioB, ΔyigM, and ΔbioB ΔyigM mutants, we next analysed the importance of the biotin biosynthesis and transport pathways for the growth and survival of S. Typhimurium within the intracellular environment. To this end, we determined the proliferation of each of the mutant strains following infection of HeLa epithelial and RAW264.7 macrophage-like cell lines. Our results revealed that the de novo biosynthesis of biotin is required for the optimal growth of S. Typhimurium following infection of both epithelial and macrophage-like cell lines. Disruption of biotin transport, by contrast, had no significant effect on the intracellular proliferation of S. Typhimurium when a functional pathway for the biosynthesis of biotin was present. The simultaneous disruption of biotin biosynthesis and transport, however, resulted in significant attenuation of S. Typhimurium in epithelial cells, while bacterial survival in macrophages decreased to below the limit of detection. Overall, our results suggest the S. Typhimurium relies primarily on biotin produced by the de novo biosynthesis pathway to support its growth in the intracellular environment. While YigM-mediated biotin transport is essential for sustaining the viability of intracellular S. Typhimurium in the absence of de novo biosynthesis, it appears to play a relatively minor role in the acquisition of biotin during growth in the nutrient-limited Salmonella containing vacuole. Our findings suggest that inhibiting biotin biosynthesis may be a viable strategy for combating systemic infections caused by Salmonella, as has been recently proposed for other medically important bacterial pathogens (Carfrae et al., 2020). , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
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- Date Issued: 2021-10-29
An empirical analysis of the asset price monetary policy transmission channel in South Africa
- Authors: Du Preez, Wilhelmus Petrus
- Date: 2021-10
- Subjects: Monetary policy South Africa , Banks and banking, Central South Africa , Transmission mechanism (Monetary policy) South Africa , Capital assets pricing model , Securities South Africa , Stocks Prices South Africa , Repurchase agreements South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190318 , vital:44983
- Description: The asset pricing channel of monetary policy transmission breeds an interesting study. There has been an ongoing debate to determine whether monetary policy committees should directly influence the asset pricing channel through changes in the central bank control interest rate or whether monetary policy committees should refrain from targeting the asset pricing channel to meet their policy objectives. The study aims to critically analysis the asset pricing channel, firstly on a global context to set the scene and then to critically focus on the study performed by Muroyiwa et al. (2017) who conducted a study on the South African monetary policy and its interdependence on the stock market through utilisation of an SVAR model. It was concluded that monetary policy does significantly influence stock prices. The purpose of this study is to build on the results which have been formed by Muroyiwa et al. (2017) and to expand on the period under analysis which encapsulates 1999:01 to 2007:12 in order to conclude whether there are conflicting conclusions or conclusions which build on what has already been done. The results from the research shows that when the monetary policy committee decides to influence changes in the repo rate, the repo rate will have a direct effect on the asset markets, the indexes that were used to represent the asset market are the all share index, industrial 15 index and the financial 15 index. Furthermore. The study which has expanded the period under analysis has supplemented and built on the results provided by Muroyiwa et al. (2017). , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2021
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- Date Issued: 2021-10
Movement behaviour and reproductive biology of adult spotted grunter (Pomadasys commersonnii) in the Breede Estuary
- Authors: Ziko, Bantony Alford
- Date: 2021-06
- Subjects: Fishery management , Freshwater fishes , Fishes--Nutrition
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20970 , vital:46870
- Description: Movements of the adult spotted grunter (Pomadasys commersonnii), an overexploited estuary-dependant fishery species, were investigated in relation to its reproduction behaviour and selected environmental influences. The South African P. commersonnii population is known to spawn at sea along the KwaZulu-Natal (KZN) coastline, whereas there no published records on spawning in the Eastern and Western Cape Provinces exist. However, due to the occurrence of ripe running fish that were reported in the Breede Estuary (Western Cape - WC), an investigation into its reproductive behaviour was necessary, as was gaining a greater understanding of its movements between the estuary, the sea, and other connected habitats. Seven adult P. commersonnii (610 – 690 mm fork length, FL) were captured and implanted with long-life acoustic transmitters and their movements were logged on a linear array of 16 acoustic receivers in the Breede Estuary (WC) and six acoustic receivers in the adjacent marine environment. Movements of the tagged fish to other marine and estuary habitats were also determined using data collected by the Acoustic Tracking Array Platform (ATAP) which is an extended network of acoustic receivers along the South African coastline with receivers at sea and in selected estuaries. Furthermore, adult P. commersonnii tagged in the Goukou (n = 6) (WC) and Sundays (n = 8) (Eastern Cape - EC) estuaries, approximately 50 km and 470 km away, respectively, were also monitored to facilitate an improved understanding of inter-habitat connectivity. Fish tagged in Sundays and Goukou estuaries were monitored by the Acoustic Telemetry Array Platform (ATAP). Each individual fish tagged in the Breede Estuary was monitored between 356 and 1166 days and selected environmental parameters, including water temperature, river flow rate and rainfall, were measured during the study period (November 2016 – March 2020) to assess their influence on the movements of the tagged fish. Furthermore, to determine time of spawning and duration, gonads of 112 adult fish (383 – 700 mm FL) were opportunistically collected from recreational anglers in the Breede Estuary to determine the gonadal maturation cycles by using a combination of Gonado-somatic index (GSI) and histology. The tagged adult P. commersonnii spent significantly more time in the estuary (83.5 percent) than the marine (16.5 percent) environment, with all of the tagged P. commersonnii making sea trips of varying frequency and duration. While in the estuary, fish showed a strong preference for the lower reaches of the estuary compared to the middle and upper reaches. Area use varied seasonally, with the mouth area being heavily utilized during winter, then followed by summer. Sea trips in all three tagging estuaries also fluctuated seasonally, with more frequent movements during summer which lasted for longer periods. This coincided with the peak of the spawning period inferred from the gonad developmental cycle of fish captured in the Breede Estuary. Results from a generalized linear mixed model showed that the presence of Breede tagged P. commersonnii in the sea, where the species is known to spawn, was significantly influenced by water temperatures in the estuary and river flow rate with fish more likely to occur at sea during warmer months (summer) and periods of high river flow (winter). Furthermore, circular analysis showed that season (month) had a significant influence on the departure from and arrival in the Breede Estuary, with most departures and arrivals occurring during January. Moreover, P. commersonnii tagged in the Sundays Estuary also showed similar results where both departures and arrivals were influenced by season, mostly taking place in December and January, respectively. The increased frequency of sea trips by adult P. commersonnii in summer suggests regional spawning occurring in the WC. Based on the similar movement behaviour displayed by tagged fish in the Sundays Estuary, it is possible that spawning also occurs in the EC. Most of the P. commersonnii tagged in all the three estuaries never left their tagging estuaries (57 percent Breede, 66 percent Goukou, 38 percent Sundays), only making short sea trips, while others displayed use of multiple habitats. On average, the tagged P. commersonnii that left their tagging estuaries travelled a maximum distance of 377 km (Breede), 63 km (Goukou) and 300 km (Sundays). Tagged fish were more likely to visit nearby estuaries and offshore marine habitats (< 130 km) compared to distant ones (> 130 km). These results suggest that there are high levels of localised connectivity between P. commersonnii populations in different habitats although connectivity between distant habitats appears to be rare. Connectivity will likely occur on habitats located close to the boundaries of the WC, EC and KZN. The findings from this long-term monitoring study have shown that adult P. commersonnii rely heavily on the estuarine environment, undertaking short sea trips, thus demonstrating the importance of estuaries to the adult population. Due to various threats faced by estuaries such as habitat degradation and overexploitation, management of these systems is paramount to conserve such fishery resources. Due to the resident behaviour of the P. commersonnii within estuaries, Marine Protected Areas (MPAs) and area closures, in conjunction with Estuarine Protected Areas (EPAs) will be significant in the recovery and management of P. commersonnii populations. Since P. commersonnii appears to reach peak spawning activity during summer when fishing pressure is usually highest, improved enforcement during this period is necessary to conserve the spawning stock. As connectivity is an important aspect in the life of the P. commersonnii, ensuring such connectivity is maintained by protecting all these habitats is important. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
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- Date Issued: 2021-06
Challenges experienced by xhosa single mothers during the traditional circumcision of their adolescent sons
- Authors: Mdunyelwa, Akhona
- Date: 2020-07
- Subjects: Circumcision
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/21004 , vital:46891
- Description: Culturally, amongst Amaxhosa, traditional male circumcision is known to be a men subject and that women are supposed to be passive and submissive in the entire process (Kang’ethe & Nomngcoyiya, 2016). Women play a huge role in nurturing children, however, when it comes to traditional male circumcision of their sons, their role is limited to preparing food and traditional beer for the initiation ceremony. These women include single mothers, therefore, the interest of this study is on how Xhosa single mothers encounter challenges in the traditional circumcision of their sons, thus the main purpose of the study was to investigate the challenges experienced by Xhosa single mothers during traditional circumcision of their adolescent sons. The following three objectives were set out to guide the investigation A. To investigate challenges experienced by Xhosa single mothers during the traditional circumcision of their adolescent sons. B. To determine the extent to which Xhosa single mothers participate in the plans and decision making during the circumcision of their adolescent sons. C. To explore the support that Xhosa single mothers require during the process of their sons’ circumcision. A purposive sampling procedure was employed, and ten Xhosa single mothers were interviewed on a one-to-one basis. In addition, a focus group consisting of four men was conducted. The following are the main findings of the study: •Traditional male circumcision is the most respected custom amongst the AmaXhosa nation. Women are in fear of speaking publicly about the challenges they experience in traditional male circumcision processes. • There are various challenges experienced by Xhosa single mothers which include the need for involvement of men to usher their sons into the ritual. • The absence and lack of support from the biological fathers of the sons results in aconsiderable burden for the Xhosa single mothers that includes financial challenges. • The religious orientation or Christian faith of some of the Xhosa single mothers clashes with the request of their sons to participate in traditional male circumcision. • The individuals and societal drop in cultural standards and being stormed by the dominant Western culture as opposed to Ubuntu was identified as the greatest challenge in the Xhosa communities. • There is a clash of cultural norms and values with the current equality lifestyle as well as the enjoyment of constitutional rights by the Xhosa single mothers during traditional circumcision of their sons. There is a lack of support for Xhosa single mothers in relation to traditional circumcision of their adolescent sons from the families, community, and the government. Much of the support is directed to strategies to preserve the custom, improve the procedure and to curb the casualties. • The study proved that, traditional male circumcision is a stressful process for the Xhosa single mothers which inflict strain and anxiety. Some of the concepts from Bowen`s family systems theory were proven and discussed as part of the findings. , Thesis (MSoc) -- Faculty of Social Science and Humanities, 2020
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- Date Issued: 2020-07
Implication of family therapy in the South African context: a case of New Brighton, Port Elizabeth.
- Authors: Mncono, Noxolo Alicia
- Date: 2020-01
- Subjects: Family psychotherapy
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/21081 , vital:46969
- Description: The objectivity of this investigation layered on the establishment of the implication of family therapy in the South African context in New Brighton, Port Elizabeth. The aims of the study were (i) To determine the traditional ways of dealing with physical, emotional and psychosocial problems in IsiXhosa speaking communities in New Brighton, Port Elizabeth and, (ii) To explore the influence of traditional beliefs in the process of family therapy amongst IsiXhosa speaking communities in New Brighton, Port Elizabeth. The interest of this study originates from the side lining of the domination of Western European- oriented healing models which tends to question the infiltration of African therapeutic interventions focusing on the Xhosa cultural practices in facing human adversities specifically in New Brighton. A qualitative research design was utilized to collect data through semi-structured interviews, focus group and observation with an aim to justify the above mentioned objectives. The sample size consisted of 16 participants i.e. 11 family elders and 5 traditional healers as a result, data obtained was analysed through thematic analysis. This study investigated the influence of culture on health behaviours of isiXhosa speaking communities through the guidance of the PEN-3 cultural framework by Collins Airhihenbuwa. Furthermore, amaXhosa consult God (through prayer and attending church), ancestors (through rituals and cultural practises), family elders (for advice and guidance) and resort to traditional healers (for physical, emotional, psychological and spiritual problems). Moreover, amaXhosa routinely conduct family meetings consisting of extended families and apply their wisdom and experience in the provision of a solution to family related problems. Based on the overall analysis and identified gaps within this field of study it is recommended that an extensive research should emphasise on ways of the collaboration of African and Western perspectives on psychological counselling. , Thesis (MSoc Sci) (Psychology) -- University of Fort Hare, 2021
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- Date Issued: 2020-01