- Title
- Evaluation of medicinal potential of Boophone disticha (L.f.) Herb. used by the indigenous people in the Raymond Mhlaba Municipality Eastern Cape
- Creator
- Gwayi, Yamkela
- Subject
- Bulbs (Plants)
- Subject
- Bulbs (Plant anatomy)
- Date Issued
- 2021-09
- Date
- 2021-09
- Type
- Master's theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/20612
- Identifier
- vital:46375
- Description
- Boophone disticha (L.f.) Herb. is a bulbous plant native to southern African areas of the continent and spreads to tropical Africa. The plant has been noted in literature for its high toxicity and has a long lineage of its use in traditional medicine for the treatment of various diseases. Due to high demand in the conventional trade market, the plant's conservation status has been shown to be declining. The plant has been reported to cause human poisoning, which can lead to death, but the plant is still administered by an indigenous community to treat various diseases. This study investigated the therapeutic potential of B. disticha used by the people of Raymond Mhlaba municipality to show and verify its use in traditional medicine and how it can be used as a possible drug ingredient. This study was also set out to investigate the anti-mycobacterial activity of the plant for the first time for the B. disticha growing in the Eastern Cape region of South Africa. The phytochemical analysis of B. disticha was carried out on four different plant parts (i.e., roots, leaves, bulb inner and outer scales) and the plant was extracted using methanol and distilled water. The qualitative phytochemical analysis displayed the presence of tannins, flavonoids, phenols and alkaloids in all plant parts for both extraction solvents. Anthraquinones were absent in all the plant extracts. The detected phytochemicals (tannins, flavonoids, phenols and alkaloids) were quantified. The roots had the highest phytochemical content for methanol extract for each phytochemical tested [439.67±1.53 mg/g (QE) flavonoids, 2414.67±1.53 mg/g (GAE) phenols and 527.33±2.08 mg/g (GAE) alkaloids] compared to all the plant parts. Methanol extracts from B. disticha revealed highest phenolic contents (2414.67±1.53 mg/g GAE) for the roots, 1395.33±2.52 mg/g (GAE) for the bulb inner scales, 1560±1 mg/g (GAE) for the leaves and 1550.7±18.9 mg/g (GAE) for the bulb outer scales). The total flavonoid content of the bulb outer scales aqueous extract was not detected. The presence of significant amounts of phytochemical compounds indicates that B. disticha has a higher medicinal value and can be extensively investigated to extract bioactive ingredients that are useful to the society, and that could be sold for higher production than using synthetic drugs with side effects. The antimicrobial activity of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well micro-plates against nine disease-causing bacterial strains (5 Gram-negative and 4 Gram-positive) and three fungal isolates. The results revealed that methanol and aqueous extract of B. disticha demonstrated very good activity, with Gram-positive strains being more sensitive than Gram-negative ones. Boophone disticha aqueous extracts displayed the best activity against Staphylococcus aureus with MIC and MBC values ranging from 0.39 to 0.78 mg/ml. The methanolic extract of B. disticha leaves and outer scales of bulb displayed good activity against Klebsiella pneumoniae at MIC 0.78 mg/ml. The methanolic extract of B. disticha bulb outer scales also displayed good inhibition against Proteus vulgaris at MIC 0.78 mg/ml, with the root extract exhibiting activity against Shigella flexineri (MIC value of 0.078 mg/ml). Concerning antifungal activity, B. disticha extracts showed very poor inhibition properties against the fungal isolates. Anti-mycobacterium potential of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well microtiter plates. The plant parts tested were the roots, bulb inner scales, leaves and the bulb outer scales. The highest activity against Mycobacterium tuberculosis was observed with the root methanol extract at MIC 0.78 mg/ml. The anti-inflammatory properties of B. disticha were investigated using the 5-lypoxygenase (5- LOX) assay. The overall anti-inflammatory activity results for the B. disticha extracts were poor; at low concentrations, the plant displayed negative results. The leaves methanol extracts did show little activity at 0.4 mg/ml.
- Description
- Thesis (MSc) (Botany) -- University of Fort Hare, 2021
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (98 pages)
- Format
- Publisher
- University
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
- Rights
- All Rights Reserved
- Rights
- Open Access
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UFH Department of Botany
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Dissertation_Final edt 2021 yamkela GWAYI.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |