Isolation and characterisation of lignocellulose degrading bacteria from Tyume River in the Eastern Cape Province, South Africa
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
Constructions of masculinity in young men's narratives of violence in the homeplace
- Authors: Stride, Lorenzo
- Date: 2008
- Subjects: Family violence , Masculinity , Men -- Identity , Men -- Psychology
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11847 , http://hdl.handle.net/10353/185 , Family violence , Masculinity , Men -- Identity , Men -- Psychology
- Description: This research was undertaken with a view to advancing scholarship on the production and reproduction of notions of masculinity through everyday experiences of violence in the domestic sphere. In particular, the researcher sought to explicate constructions of masculinity in men’s narratives of their experiences of violence in the homeplace. The participants in this study constituted a fairly homogenous sample in terms of age, education, geographic location, and socio-economic status. A homogenous sample was purposefully selected because it aided an analysis of the phenomenon under study without diversions from extraneous variables. Data was collected from semi-structured, personal, in-depth, face-to-face interviews with eight young men. In these interviews participants were asked to recall and to talk about one particular experience of domestic violence that they witnessed or that had happened to them in the past. Photo elicitation was used as a reflective technique aimed at facilitating recall and discussion during the interviews. Data was analysed by means of a discourse analysis. The main findings of this research were that the participants grew up in communities where a more traditional hegemonic masculinity was commonplace and where violence as a means of exerting control was associated with being a ‘real man’. The participants did however question this notion of masculinity as a result of their experiences, particularly when they perceived the violence that they had been exposed to as excessive or unwarranted.
- Full Text:
- Date Issued: 2008
- Authors: Stride, Lorenzo
- Date: 2008
- Subjects: Family violence , Masculinity , Men -- Identity , Men -- Psychology
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11847 , http://hdl.handle.net/10353/185 , Family violence , Masculinity , Men -- Identity , Men -- Psychology
- Description: This research was undertaken with a view to advancing scholarship on the production and reproduction of notions of masculinity through everyday experiences of violence in the domestic sphere. In particular, the researcher sought to explicate constructions of masculinity in men’s narratives of their experiences of violence in the homeplace. The participants in this study constituted a fairly homogenous sample in terms of age, education, geographic location, and socio-economic status. A homogenous sample was purposefully selected because it aided an analysis of the phenomenon under study without diversions from extraneous variables. Data was collected from semi-structured, personal, in-depth, face-to-face interviews with eight young men. In these interviews participants were asked to recall and to talk about one particular experience of domestic violence that they witnessed or that had happened to them in the past. Photo elicitation was used as a reflective technique aimed at facilitating recall and discussion during the interviews. Data was analysed by means of a discourse analysis. The main findings of this research were that the participants grew up in communities where a more traditional hegemonic masculinity was commonplace and where violence as a means of exerting control was associated with being a ‘real man’. The participants did however question this notion of masculinity as a result of their experiences, particularly when they perceived the violence that they had been exposed to as excessive or unwarranted.
- Full Text:
- Date Issued: 2008
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