Application of systems theory for analysing the sustainability foci and practices of the Faculty of Economics and Information Technology Systems: (A Case Study of Walter Sisulu University: Komani campus)
- Authors: Klishi, Konwaba Bomikazi
- Date: 2022-10-14
- Subjects: Sustainable development Study and teaching (Higher) South Africa Mthatha , System theory , Curriculum evaluation South Africa Mthatha , Community engagement , Walter Sisulu University for Technology and Science
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/405281 , vital:70157
- Description: Sustainable development is a global concern that intertwines several factors, including poverty, biodiversity, inequality, water, and energy. This interconnection does not only affect the current generation but may have long term implications for future generations as we see the rapid spread of environmental risk in our local communities. In this study, systems theory was adapted for analysing the sustainability focus of the Faculty of Economics and Information Technology Systems (FEITS) at Walter Sisulu University. A qualitative research method was used during this research, and data collected from a purposive sample of staff, activities and documentation. In this study, Von Bertalanffy’s systems theory is applied to analyse the sustainability foci and practices of the FEITS. The study describes sustainability practices and concerns that emerge from research activities, community engagement activities and work-integrated learning. The theory is also used to analyse the connection between various subsystems of the faculty when addressing emerging local sustainability concerns. Education for sustainable development is an important aspect of this study as it considers the sustainability concerns in the curriculum from the perspective of current and future alternatives for the economy and socio-ecological aspects of communities. The following findings have emerged from the study: Contextual realities related to inequality were foci of discussion and concern in various qualifications. Socioecological issues and risks were found to be related to water and sanitation, food security and climate change. Sustainability discourse is related to health and wellbeing, peace, and security. There is an alignment of green skills to the curriculum of the faculty and pedagogical practices which relate to sustainability. The faculty participates in stakeholder platforms to respond local sustainability challenges and is developing courses in response to local development needs. This study has implications for the faculty’s curriculum in terms of the content of various qualifications, future research, and community engagement activities of the university. , Thesis (MEd) -- Faculty of Education, Education, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Klishi, Konwaba Bomikazi
- Date: 2022-10-14
- Subjects: Sustainable development Study and teaching (Higher) South Africa Mthatha , System theory , Curriculum evaluation South Africa Mthatha , Community engagement , Walter Sisulu University for Technology and Science
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/405281 , vital:70157
- Description: Sustainable development is a global concern that intertwines several factors, including poverty, biodiversity, inequality, water, and energy. This interconnection does not only affect the current generation but may have long term implications for future generations as we see the rapid spread of environmental risk in our local communities. In this study, systems theory was adapted for analysing the sustainability focus of the Faculty of Economics and Information Technology Systems (FEITS) at Walter Sisulu University. A qualitative research method was used during this research, and data collected from a purposive sample of staff, activities and documentation. In this study, Von Bertalanffy’s systems theory is applied to analyse the sustainability foci and practices of the FEITS. The study describes sustainability practices and concerns that emerge from research activities, community engagement activities and work-integrated learning. The theory is also used to analyse the connection between various subsystems of the faculty when addressing emerging local sustainability concerns. Education for sustainable development is an important aspect of this study as it considers the sustainability concerns in the curriculum from the perspective of current and future alternatives for the economy and socio-ecological aspects of communities. The following findings have emerged from the study: Contextual realities related to inequality were foci of discussion and concern in various qualifications. Socioecological issues and risks were found to be related to water and sanitation, food security and climate change. Sustainability discourse is related to health and wellbeing, peace, and security. There is an alignment of green skills to the curriculum of the faculty and pedagogical practices which relate to sustainability. The faculty participates in stakeholder platforms to respond local sustainability challenges and is developing courses in response to local development needs. This study has implications for the faculty’s curriculum in terms of the content of various qualifications, future research, and community engagement activities of the university. , Thesis (MEd) -- Faculty of Education, Education, 2022
- Full Text:
- Date Issued: 2022-10-14
Assessment of cytotoxic artemisinin and its derivatives as DNA damaging inducing agents in triple-negative breast cancer cells
- Authors: Mkhwanazi, Ntando
- Date: 2022-10-14
- Subjects: Breast Cancer , Artemisinin , DNA damage , Antineoplastic agents , Breast Cancer Treatment
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362960 , vital:65378
- Description: In developing countries, including South Africa, breast cancer is the primary cause of cancer-related deaths among women. TNBC (triple-negative breast cancer) is an aggressive breast cancer subtype that is more prevalent in women of African descent. This subtype lacks the key receptors, namely the estrogen receptor (ER-), progesterone receptor (PR-), and human epidermal growth factor receptor 2 (HER2-) that are the basis of successful targeted therapies for other subtypes of the disease. To date, there are no effective, standardized targeted therapies for TNBC. Artemisinin is an anti-malarial drug and numerous derivatives of the compound have been developed to improve the potency and solubility of the parent compound. Artemisinin and its derivatives have gained attention as potential anti-cancer agents; however, such studies have not yet progressed to clinical trials and the precise mechanism of action of these compounds is yet to be fully explained. In this study, artemisinin, and its known derivative artesunate, as well as a novel derivative, WHN11, were investigated as DNA damage-inducing agents in TNBC. WHN11 was found to be the most potent of the three compounds, displaying an IC50 of 3.20 μM against HCC70 cells, artemisinin displayed an IC50 of 214.70 μM and artesunate displayed an IC50 of 25.48 μM. The compounds were less toxic to the MCF12A non-cancerous cells, with IC50 values 298.30, 87.53, and 8.35 μM for artemisinin, artesunate, and WHN11, respectively, and displayed selectivity indices of 1.39, 3.44 and 2.61 μM for artemisinin, artesunate, and WHN11, respectively. In silico and in vitro studies revealed that the artemisinin compounds bind to DNA through the minor groove. While all three compounds were able to bind to DNA, a comet assay revealed that only artemisinin and artesunate, and not WHN11, were able to cause DNA damage compared to the vehicle control, DMSO. Finally, a topoisomerase I (TOPO I) enzyme assay demonstrated that while the compounds appeared to display a degree of inhibition of TOPO I, as evidenced by a downward shift in the plasmid band on the agarose gel, they were not able to fully inhibit the enzyme to return the plasmid to the supercoiled conformation. In addition, combination studies revealed that artemisinin, artesunate, and WHN11 acted synergistically in combination with camptothecin, but displayed either an additive (artemisinin) or antagonistic (artesunate and WHN11) relationship when used in combination with etoposide. In conclusion, artemisinin, its known derivative artesunate, and novel and highly toxic derivative WHN11, all bind to DNA via the minor groove, however only artemisinin and artesunate, and not WHN11, cause DNA damage, indicating a potentially different mechanism of action of the three artemisinins. All three compounds act synergistically with camptothecin, which suggests interference with topoisomerase activity, partially supported by slight inhibition of TOPO I activity, and could indicate either direct inhibition of the enzyme or interference with enzyme function by competitive binding to the DNA. Further studies could help explore alternate DNA damage assays, to validate these findings, and the effect of the compounds on TOPO II activity could also be assessed. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Mkhwanazi, Ntando
- Date: 2022-10-14
- Subjects: Breast Cancer , Artemisinin , DNA damage , Antineoplastic agents , Breast Cancer Treatment
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362960 , vital:65378
- Description: In developing countries, including South Africa, breast cancer is the primary cause of cancer-related deaths among women. TNBC (triple-negative breast cancer) is an aggressive breast cancer subtype that is more prevalent in women of African descent. This subtype lacks the key receptors, namely the estrogen receptor (ER-), progesterone receptor (PR-), and human epidermal growth factor receptor 2 (HER2-) that are the basis of successful targeted therapies for other subtypes of the disease. To date, there are no effective, standardized targeted therapies for TNBC. Artemisinin is an anti-malarial drug and numerous derivatives of the compound have been developed to improve the potency and solubility of the parent compound. Artemisinin and its derivatives have gained attention as potential anti-cancer agents; however, such studies have not yet progressed to clinical trials and the precise mechanism of action of these compounds is yet to be fully explained. In this study, artemisinin, and its known derivative artesunate, as well as a novel derivative, WHN11, were investigated as DNA damage-inducing agents in TNBC. WHN11 was found to be the most potent of the three compounds, displaying an IC50 of 3.20 μM against HCC70 cells, artemisinin displayed an IC50 of 214.70 μM and artesunate displayed an IC50 of 25.48 μM. The compounds were less toxic to the MCF12A non-cancerous cells, with IC50 values 298.30, 87.53, and 8.35 μM for artemisinin, artesunate, and WHN11, respectively, and displayed selectivity indices of 1.39, 3.44 and 2.61 μM for artemisinin, artesunate, and WHN11, respectively. In silico and in vitro studies revealed that the artemisinin compounds bind to DNA through the minor groove. While all three compounds were able to bind to DNA, a comet assay revealed that only artemisinin and artesunate, and not WHN11, were able to cause DNA damage compared to the vehicle control, DMSO. Finally, a topoisomerase I (TOPO I) enzyme assay demonstrated that while the compounds appeared to display a degree of inhibition of TOPO I, as evidenced by a downward shift in the plasmid band on the agarose gel, they were not able to fully inhibit the enzyme to return the plasmid to the supercoiled conformation. In addition, combination studies revealed that artemisinin, artesunate, and WHN11 acted synergistically in combination with camptothecin, but displayed either an additive (artemisinin) or antagonistic (artesunate and WHN11) relationship when used in combination with etoposide. In conclusion, artemisinin, its known derivative artesunate, and novel and highly toxic derivative WHN11, all bind to DNA via the minor groove, however only artemisinin and artesunate, and not WHN11, cause DNA damage, indicating a potentially different mechanism of action of the three artemisinins. All three compounds act synergistically with camptothecin, which suggests interference with topoisomerase activity, partially supported by slight inhibition of TOPO I activity, and could indicate either direct inhibition of the enzyme or interference with enzyme function by competitive binding to the DNA. Further studies could help explore alternate DNA damage assays, to validate these findings, and the effect of the compounds on TOPO II activity could also be assessed. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
Baseline assessments for foundation phase reading in isiXhosa: a case study
- Authors: Nesi, Nolubabalo Amanda
- Date: 2022-10-14
- Subjects: Test of Early Reading Ability , Xhosa language , Reading (Elementary) Ability testing , Reading (Elementary) Social aspects South Africa Makhanda , Dynamic assessment (Education)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406320 , vital:70258
- Description: The main objective of this study was to investigate the use of baseline assessments for reading in isiXhosa in Foundation Phase. It focused on how teachers conduct baseline assessments, the assessment tools they use, and their responsiveness to baseline assessments data in terms of curriculum planning and practice, given the lack of explicit guidelines and tools specifically developed for African languages. The lack of clarity may lead teachers to assume that the same assessment approaches and practices apply across all official languages despite their linguistic and sociolinguistic differences. Vygotsky’s socio-cultural theory of learning and Biggs’ concept on constructive alignment are used as the theoretical and conceptual framework for this study. The premise for this study was that it would be problematic to apply similar assessment approaches and use the same tools for all official South African languages. The socio-cultural approach emphasises the importance of language learning and learning generally according to the context within which language occurs. The main research sites are two Quintile 1 schools in a township in Makhanda District. These schools have been receiving external intervention which includes a baseline assessment for isiXhosa reading from Non-Governmental Organisations (NGOs). This study sought to provide some insights on whether previous assessments have informed subsequent teaching practices. The findings of the study revealed that the most prominent tool for assessing reading in isiXhosa is Early Grade Reading Assessment (EGRA) and the tools used by external literacy interventions in the two schools were adapted versions of EGRA. Additionally, the subtasks in the baseline assessment tools used were aligned to the linguistic features of isiXhosa besides the nonsense word component. Furthermore, teachers were assessing and teaching reading at lower levels than expected in the grades 2 and 3 in this study. This study recommends that teachers respond to the baseline assessment data appropriately to develop reading competencies. In addition, there is need for an alignment between the linguistic features, the curriculum and strategies of teaching reading. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Nesi, Nolubabalo Amanda
- Date: 2022-10-14
- Subjects: Test of Early Reading Ability , Xhosa language , Reading (Elementary) Ability testing , Reading (Elementary) Social aspects South Africa Makhanda , Dynamic assessment (Education)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406320 , vital:70258
- Description: The main objective of this study was to investigate the use of baseline assessments for reading in isiXhosa in Foundation Phase. It focused on how teachers conduct baseline assessments, the assessment tools they use, and their responsiveness to baseline assessments data in terms of curriculum planning and practice, given the lack of explicit guidelines and tools specifically developed for African languages. The lack of clarity may lead teachers to assume that the same assessment approaches and practices apply across all official languages despite their linguistic and sociolinguistic differences. Vygotsky’s socio-cultural theory of learning and Biggs’ concept on constructive alignment are used as the theoretical and conceptual framework for this study. The premise for this study was that it would be problematic to apply similar assessment approaches and use the same tools for all official South African languages. The socio-cultural approach emphasises the importance of language learning and learning generally according to the context within which language occurs. The main research sites are two Quintile 1 schools in a township in Makhanda District. These schools have been receiving external intervention which includes a baseline assessment for isiXhosa reading from Non-Governmental Organisations (NGOs). This study sought to provide some insights on whether previous assessments have informed subsequent teaching practices. The findings of the study revealed that the most prominent tool for assessing reading in isiXhosa is Early Grade Reading Assessment (EGRA) and the tools used by external literacy interventions in the two schools were adapted versions of EGRA. Additionally, the subtasks in the baseline assessment tools used were aligned to the linguistic features of isiXhosa besides the nonsense word component. Furthermore, teachers were assessing and teaching reading at lower levels than expected in the grades 2 and 3 in this study. This study recommends that teachers respond to the baseline assessment data appropriately to develop reading competencies. In addition, there is need for an alignment between the linguistic features, the curriculum and strategies of teaching reading. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
Big T’s and small T’s: an explorative study on trauma narratives in South Africa
- Authors: Naidoo, Rinisa
- Date: 2022-10-14
- Subjects: Psychic trauma , Anthropology , Mental health South Africa , Apartheid South Africa Personal narratives , Culture Psychological aspects
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408641 , vital:70512
- Description: The 21st century has seen a dramatic increase in chronic non-infectious diseases, especially in the area of mental health. Medical anthropologists have seen a rise in the development of mental illnesses in both developed and developing nations. There is, however, little research conducted on trauma narratives that do not stem from political violence from an anthropological lens. South Africa has various understandings of trauma depending on the cultural context and it is crucial to examine these narratives as this provides vital information of the daily lived experiences of trauma survivors. Key themes draw on issues of trauma denialism, communicating distress, traumatic symptoms and the development of mental illnesses as a result of traumatic exposure. The data was analysed through Goffman’s (1959) Presentation of Self in Everyday Life illustrating various ways how survivors present themselves depending on the particular audience. This research employed both qualitative and quantitative research methods to gather a holistic understanding of trauma survivors. With the use of semi-structured interviews of Stressful Life Events Questionnaire coupled with observations of online support groups for trauma survivors, this research has provided rich ethnographic evidence of the impact that culture has on trauma narratives illustrating a clear normalcy of trauma present in South Africa. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Naidoo, Rinisa
- Date: 2022-10-14
- Subjects: Psychic trauma , Anthropology , Mental health South Africa , Apartheid South Africa Personal narratives , Culture Psychological aspects
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408641 , vital:70512
- Description: The 21st century has seen a dramatic increase in chronic non-infectious diseases, especially in the area of mental health. Medical anthropologists have seen a rise in the development of mental illnesses in both developed and developing nations. There is, however, little research conducted on trauma narratives that do not stem from political violence from an anthropological lens. South Africa has various understandings of trauma depending on the cultural context and it is crucial to examine these narratives as this provides vital information of the daily lived experiences of trauma survivors. Key themes draw on issues of trauma denialism, communicating distress, traumatic symptoms and the development of mental illnesses as a result of traumatic exposure. The data was analysed through Goffman’s (1959) Presentation of Self in Everyday Life illustrating various ways how survivors present themselves depending on the particular audience. This research employed both qualitative and quantitative research methods to gather a holistic understanding of trauma survivors. With the use of semi-structured interviews of Stressful Life Events Questionnaire coupled with observations of online support groups for trauma survivors, this research has provided rich ethnographic evidence of the impact that culture has on trauma narratives illustrating a clear normalcy of trauma present in South Africa. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
- Date Issued: 2022-10-14
Bioactivity evaluation of manno-oligosaccharides produced from spent coffee grounds using a Bacillus sp. derived endo-1,4-β-mannanase
- Authors: Magengelele, Mihle
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365233 , vital:65719
- Description: Thesis embargoed. Possible release date set for early 2024. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Magengelele, Mihle
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365233 , vital:65719
- Description: Thesis embargoed. Possible release date set for early 2024. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
Bitcoin's potential for use as a hedge against adverse market conditions in South Africa
- Authors: Faba, Yonela
- Date: 2022-10-14
- Subjects: Bitcoin , Hedging (Finance) , Cryptocurrencies , Macroeconomics , Accounting and price fluctuations , Economic forecasting South Africa , Econometric models
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/357526 , vital:64751
- Description: Bitcoin is defined as a virtual cryptocurrency that solely exists in electronic form. Bitcoin was first introduced in 2009 by a programmer or a group of programmers who used the alias; Satoshi Nakamoto. Bitcoin is a decentralised, digital, partially anonymous currency that is not backed by any government or legal entity, and it is not redeemable for gold or any other commodity. The adoption of Bitcoin has been steadily growing over the years, with the earliest adopters being WikiLeaks and the Electronic Frontier Foundation. Ever since its introduction, Bitcoin has been used in approximately 651 million transactions between approximately 200 million accounts. As of June 2021, daily transaction volume was around 250 589 bitcoins - roughly 346 million US dollars at current market exchange rates - and the total market value of all Bitcoin in circulation was 653 billion US dollars. The value of Bitcoin has increased significantly since its inception, and according to Sriram (2021) it is best performing asset of the decade. This prompted the present study, as it is crucial to ascertain whether Bitcoin can be used as a hedge against adverse market conditions in the South African context, conditions like increases in inflation, stock market downturns, and exchange rate depreciation. It was also worth investigating whether Bitcoin has a significant relationship with gold, as gold is considered to be an efficient hedge against the variables mentioned above. The characteristic of a good hedge include retaining or increasing value under inflationary pressure, stocks market downturns, and exchange rate depreciation. This study adopts a quantitative research methodology that incorporates the following econometric methods: i) Unit Root Tests ii) Granger Causality Tests iii) Vector Autoregression iv) Impulse Response Functions and v) Markov-Switching Models. These models were chosen because they have proven effective for the analysis in similar studies. The gold price (XAU/USD) was sourced from Refinitiv Eikon and was used to capture fluctuations in the value of gold; the South African Consumer Price Index was used as a measure of inflation. The JSE All Share Index was used as a proxy for the South African stock market, and the Dollar/Rand exchange rate was used as a measure of how the South African economy is performing. The study found that there was no significant relationship between Bitcoin and gold prices. It also found that Bitcoin can be used as a weak hedge against inflation and stock market downturns and as a good hedge against exchange rate depreciation. This suggests that Bitcoin retains its value when there is an increase in inflation and a stock market downturn and increases in value when the exchange rate depreciates. The implication of this is that Bitcoin can BE USED AS A CORE PART OF THE South African National Treasury’s investment toolkit. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Faba, Yonela
- Date: 2022-10-14
- Subjects: Bitcoin , Hedging (Finance) , Cryptocurrencies , Macroeconomics , Accounting and price fluctuations , Economic forecasting South Africa , Econometric models
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/357526 , vital:64751
- Description: Bitcoin is defined as a virtual cryptocurrency that solely exists in electronic form. Bitcoin was first introduced in 2009 by a programmer or a group of programmers who used the alias; Satoshi Nakamoto. Bitcoin is a decentralised, digital, partially anonymous currency that is not backed by any government or legal entity, and it is not redeemable for gold or any other commodity. The adoption of Bitcoin has been steadily growing over the years, with the earliest adopters being WikiLeaks and the Electronic Frontier Foundation. Ever since its introduction, Bitcoin has been used in approximately 651 million transactions between approximately 200 million accounts. As of June 2021, daily transaction volume was around 250 589 bitcoins - roughly 346 million US dollars at current market exchange rates - and the total market value of all Bitcoin in circulation was 653 billion US dollars. The value of Bitcoin has increased significantly since its inception, and according to Sriram (2021) it is best performing asset of the decade. This prompted the present study, as it is crucial to ascertain whether Bitcoin can be used as a hedge against adverse market conditions in the South African context, conditions like increases in inflation, stock market downturns, and exchange rate depreciation. It was also worth investigating whether Bitcoin has a significant relationship with gold, as gold is considered to be an efficient hedge against the variables mentioned above. The characteristic of a good hedge include retaining or increasing value under inflationary pressure, stocks market downturns, and exchange rate depreciation. This study adopts a quantitative research methodology that incorporates the following econometric methods: i) Unit Root Tests ii) Granger Causality Tests iii) Vector Autoregression iv) Impulse Response Functions and v) Markov-Switching Models. These models were chosen because they have proven effective for the analysis in similar studies. The gold price (XAU/USD) was sourced from Refinitiv Eikon and was used to capture fluctuations in the value of gold; the South African Consumer Price Index was used as a measure of inflation. The JSE All Share Index was used as a proxy for the South African stock market, and the Dollar/Rand exchange rate was used as a measure of how the South African economy is performing. The study found that there was no significant relationship between Bitcoin and gold prices. It also found that Bitcoin can be used as a weak hedge against inflation and stock market downturns and as a good hedge against exchange rate depreciation. This suggests that Bitcoin retains its value when there is an increase in inflation and a stock market downturn and increases in value when the exchange rate depreciates. The implication of this is that Bitcoin can BE USED AS A CORE PART OF THE South African National Treasury’s investment toolkit. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2022
- Full Text:
- Date Issued: 2022-10-14
Chaos!
- Ngwenya, Thembalethu Sabrina
- Authors: Ngwenya, Thembalethu Sabrina
- Date: 2022-10-14
- Subjects: Creative writing (Higher education) South Africa , Diaries -- Authorship , Books Reviews , South African prose literature (English) 21st century , South African fiction (English) 21st century
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406298 , vital:70256
- Description: Chaos! is a combination of short prose forms in fragments that tackles difficult topics such as troubled marriages, child molestation, rape, infidelity and abuse in every way, whether it is mental, emotional or physical. The stories contain a lot of horrific and violent acts that, often times in this day and age, happen behind closed doors or sometimes even openly, yet not a lot of people are ready to talk about these topics. In my thesis, I go into the minds of those that are abusive and those that are on the receiving end of abuse. In doing so, I aim to explore all the dynamics of abuse. Abusers, as I have portrayed in my thesis, tend to have a deranged sense of reasoning in performing abusive acts and one thing that is important to note is that, more than likely, in their own heads and according to their own reasoning, the behaviour that they act on is very much rational and reasonable. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Ngwenya, Thembalethu Sabrina
- Date: 2022-10-14
- Subjects: Creative writing (Higher education) South Africa , Diaries -- Authorship , Books Reviews , South African prose literature (English) 21st century , South African fiction (English) 21st century
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406298 , vital:70256
- Description: Chaos! is a combination of short prose forms in fragments that tackles difficult topics such as troubled marriages, child molestation, rape, infidelity and abuse in every way, whether it is mental, emotional or physical. The stories contain a lot of horrific and violent acts that, often times in this day and age, happen behind closed doors or sometimes even openly, yet not a lot of people are ready to talk about these topics. In my thesis, I go into the minds of those that are abusive and those that are on the receiving end of abuse. In doing so, I aim to explore all the dynamics of abuse. Abusers, as I have portrayed in my thesis, tend to have a deranged sense of reasoning in performing abusive acts and one thing that is important to note is that, more than likely, in their own heads and according to their own reasoning, the behaviour that they act on is very much rational and reasonable. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
Characterising the sleep wake behaviour of late adolescents living in rural and township areas surrounding Alice in the Eastern Cape of South Africa
- Authors: Dlepu, Phelokazi
- Date: 2022-10-14
- Subjects: Teenagers Sleep South Africa Alice , Rural teenagers South Africa Alice , Sleep-wake cycle
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362808 , vital:65364
- Description: Introduction: School going adolescents have been identified as a group that is at risk of obtaining insufficient sleep due to shortened and irregular sleep, all of which can affect the overall wellbeing of adolescents. While there has been extenstive research on adolescent sleep around the world, research in South Africa has been limited. Of the research performed in this context, most has been conducted in city or urban contexts, but there has been comparatively less research on sleep in adolescents from rural and township areas. Therefore, this study focused on characterising and comparing the sleep-wake behaviour of late adolescents from selected rural and township areas in Alice in the Eastern Cape of South Africa. Methods: An adapted version School Sleep Habits Survey (SSHS) was included in the study and assessed demographic, academic, sleep-wake behaviour, lifestyle, and behavioural information of adolescents from rural and township schools in and around Alice. The survey was distributed physically to all the learners from four co-educational rural and township schools. The responses to the survey were analysed with descriptive and non-parametric inferential statistics, as all data were not normally distributed. Results: A total of 123 learners completed the survey. All the participants were in Grade 12, aged 16 to 26 years (median =18, IQR= 18-19) from rural (n=28) and township (n=95) schools. Seventy-four female and 49 male learners participated in the study. Within the sample, there were also a group of participants who were not adolescents (n=22) whose age ranged between 20 and 26. Reported sleep during both the week and weekend (7hours) was lower than both the recommended duration for adolescents and their own self-reported sleep need of a median of 8 hours and 14 minutes. Bedtimes were significantly earlier on weekdays than weekends. The need to do homework and feeling sleepy were cited as the main reasons for going to sleep during the week and the weekends. Wake times on weekdays and weekends did not differ, with most learners citing being woken up by an alarm clock as the main reason for waking during both weekdays and weekends. Most learners (n=47; 38%) reported walking to get to school, while 41 (33%) relied on public transport to get to school. There were no significant effects of gender or of being rural and township scholars in relation to their sleep variables. Comparisons between non-adolescents and adolescents revealed that non-adolescents reported sleeping longer (7 hours and 30 minutes) compared to adolescents (7 hours) than adolescents did on weekends. As a group, the learners in this sample were morningness-orientated in terms of chronotype, presented with low day time sleepiness, some evidence of sleep-wake behaviour problems and of depressive mood symptoms. Nearly half (n=53; 43%) the group reported napping occasionally on school days. In terms of caffeine consumption, just over half (n=64; 52%) of the learners reported never consuming caffeinated substances such as soda, coffee, or tea. Conclusions: The reported sleep duration of learners in this study was consistent on both the week and weekend but was shorter than the recommended 8-10 hours for late adolescents. While learners in this sample reported early bedtimes, sleep was likely curtailed by the early rise times due to the contextual constraints where scholars had to commute, by either walking or taking public transport, to make the early school start times. There was a high prevalence of sleep disturbances in this sample however, the effects of this (increased daytime sleepiness) were lower which can be attributed to low reported prevalence of caffeine consumption combined with the high prevalence of reported day time napping on school days and weekends. While only a snapshot, the results of this study support previous research that has highlighted the challenges that many adolescents across the globe experience when it comes to obtaining enough sleep. The results of this study highlighted the need for more research to understand the sleep-wake behaviours of learners and their lifestyles more objectively. Given the early starts for many participants in this study, interventions such as delaying school start times may help to improve the amount of sleep that learners get during the week. However, extensive consultations would be needed to ensure that systemic changes that aim to improve sleep of adolescents are applicable for the context of rural and townships areas in South Africa. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Dlepu, Phelokazi
- Date: 2022-10-14
- Subjects: Teenagers Sleep South Africa Alice , Rural teenagers South Africa Alice , Sleep-wake cycle
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362808 , vital:65364
- Description: Introduction: School going adolescents have been identified as a group that is at risk of obtaining insufficient sleep due to shortened and irregular sleep, all of which can affect the overall wellbeing of adolescents. While there has been extenstive research on adolescent sleep around the world, research in South Africa has been limited. Of the research performed in this context, most has been conducted in city or urban contexts, but there has been comparatively less research on sleep in adolescents from rural and township areas. Therefore, this study focused on characterising and comparing the sleep-wake behaviour of late adolescents from selected rural and township areas in Alice in the Eastern Cape of South Africa. Methods: An adapted version School Sleep Habits Survey (SSHS) was included in the study and assessed demographic, academic, sleep-wake behaviour, lifestyle, and behavioural information of adolescents from rural and township schools in and around Alice. The survey was distributed physically to all the learners from four co-educational rural and township schools. The responses to the survey were analysed with descriptive and non-parametric inferential statistics, as all data were not normally distributed. Results: A total of 123 learners completed the survey. All the participants were in Grade 12, aged 16 to 26 years (median =18, IQR= 18-19) from rural (n=28) and township (n=95) schools. Seventy-four female and 49 male learners participated in the study. Within the sample, there were also a group of participants who were not adolescents (n=22) whose age ranged between 20 and 26. Reported sleep during both the week and weekend (7hours) was lower than both the recommended duration for adolescents and their own self-reported sleep need of a median of 8 hours and 14 minutes. Bedtimes were significantly earlier on weekdays than weekends. The need to do homework and feeling sleepy were cited as the main reasons for going to sleep during the week and the weekends. Wake times on weekdays and weekends did not differ, with most learners citing being woken up by an alarm clock as the main reason for waking during both weekdays and weekends. Most learners (n=47; 38%) reported walking to get to school, while 41 (33%) relied on public transport to get to school. There were no significant effects of gender or of being rural and township scholars in relation to their sleep variables. Comparisons between non-adolescents and adolescents revealed that non-adolescents reported sleeping longer (7 hours and 30 minutes) compared to adolescents (7 hours) than adolescents did on weekends. As a group, the learners in this sample were morningness-orientated in terms of chronotype, presented with low day time sleepiness, some evidence of sleep-wake behaviour problems and of depressive mood symptoms. Nearly half (n=53; 43%) the group reported napping occasionally on school days. In terms of caffeine consumption, just over half (n=64; 52%) of the learners reported never consuming caffeinated substances such as soda, coffee, or tea. Conclusions: The reported sleep duration of learners in this study was consistent on both the week and weekend but was shorter than the recommended 8-10 hours for late adolescents. While learners in this sample reported early bedtimes, sleep was likely curtailed by the early rise times due to the contextual constraints where scholars had to commute, by either walking or taking public transport, to make the early school start times. There was a high prevalence of sleep disturbances in this sample however, the effects of this (increased daytime sleepiness) were lower which can be attributed to low reported prevalence of caffeine consumption combined with the high prevalence of reported day time napping on school days and weekends. While only a snapshot, the results of this study support previous research that has highlighted the challenges that many adolescents across the globe experience when it comes to obtaining enough sleep. The results of this study highlighted the need for more research to understand the sleep-wake behaviours of learners and their lifestyles more objectively. Given the early starts for many participants in this study, interventions such as delaying school start times may help to improve the amount of sleep that learners get during the week. However, extensive consultations would be needed to ensure that systemic changes that aim to improve sleep of adolescents are applicable for the context of rural and townships areas in South Africa. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
- Date Issued: 2022-10-14
Chemostratigraphy of the lowermost iron-manganese cycle of the Hotazel Formation, and implications for its primary depositional environment
- Authors: Masoabi, Ntseka Thomas
- Date: 2022-10-14
- Subjects: Chemostratigraphy , Great Oxygenation Event , Manganese ores Geology South Africa Northern Cape , Banded iron formation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362938 , vital:65376
- Description: The giant Kalahari Manganese Field (KMF), located in the Northern Cape Province, South Africa, comprises approximately half of the world’s manganese resources, estimated at about eight billion tons at grades ranging from 20-48 wt%. The KMF is linked to a period in geological time when the Earth’s atmospheric and oceanic conditions underwent a major transition from oxygen-deficient to oxygen-enriched conditions – an event famously referred to as the Great Oxidation Event (GOE) that occurred around 2.4 Ga. The KMF deposits are hosted in Banded Iron Formation (BIF) of the Paleoproterozoic Hotazel Formation in the uppermost Transvaal Supergroup. The sedimentary Mn ores are interbedded with Hotazel BIF in the form of three alternating depositional cycles of BIF, transitional hematite lutite and laminated, carbonate-rich manganese ore. The lowermost and thickest of the three cycles is the most economically significant and has been mined for several decades on a large scale from the southernmost KMF. In this study, two drill cores from the southern KMF were inspected, logged and sampled at a high resolution of approximately half-meter interval per sample. The selected cores, namely G774, capturing the lower portion of the Hotazel Formation from the Mamatwan locality, and MP-56, capturing the corresponding portion from the Middleplaats locality, are geographically proximal to each other, with a horizontal distance of roughly 3 km separating the two of them. The G774 drill core is characterized by a conspicuously thick manganese layer covering a thickness of 50 m, with the overlying BIF reaching a total thickness of 11 m. The MP-56 drill core, on the other hand, has a relatively thinner corresponding manganese layer of 30 m in thickness, while the overlying BIF layer exhibits a thickness of 24 m. The extent of sampling up-section was constrained by an apparently coeval black shale layer which represents the chosen upper stratigraphic marker for the lower part of the Hotazel section in the broader area that is under investigation in this thesis. That way, a high resolution chemostratigraphic approach was employed to elucidate the potential factors contributing to the relative sedimentary lateral thickness variations seen across the southernmost KMF. High-resolution geochemical data were used to explore relationships and signals that might constrain relative precipitation rates for iron and manganese against detrital species, fluctuating redox conditions in the original environment of deposition, and chemostratigraphic correlation. All geochemical data (i.e., major oxides, minor and trace elements and carbonate carbon isotopes) were obtained respectively through employing X-ray Fluorescence (XRF), Laser Ablation Inductively Coupled Mass Spectrometry (ICP-MS), and Gas-source mass spectrometry. Comparative considerations made between the bulk geochemistry of the two sequences (i.e., Mamatwan and Middleplaats sections) reveal that periods of high-Mn deposition in the Hotazel Formation appear to be very Ca-carbonate rich (as indicated by high CaO, LOI and Sr concentrations). This, in turn, suggests that the Mn abundance is in the Hotazel ores is controlled mainly by the silicate phase braunite and is diluted by the deposition of Ca-carbonate through time. Bulk-rock concentration results for trace elements of the High Field Strength Element (HFSE) group (namely Zr, Hf, Y, Nb and Sc) were utilized to constrain the rates of either clastic and/or volcanic detrital inputs, as they traditionally represent refractory mineral particles of a common detrital/volcanic origin. The two chemosedimentary sequences preserve these elements in very low and thus quantitatively negligible concentrations – suggesting that the Hotazel depositional environment received very low and insignificant influx of a terrigeneous detrital component. A selection of these elements was therefore used to deduce, with caution, the relative as opposed to absolute precipitation rate of the major chemical constituents (i.e., Fe + Si vs Mn + carbonate), assuming a constant detrital flux through time. It was found that the relative abundances of Zr, Y and Nb is roughly 1.5 – 2 times as high in the BIF lithofacies relative to the Mn ones at both localities. This led to the inference that the Mn-enriched portion of the sediment must have been deposited at approximately twice the rate that the Fe-rich (BIF) portion was originally deposited. In terms of redox-sensitive elements, the elements Co and Mo seem to reveal the most valuable insights into the redox environment of primary chemical deposition. Cobalt displays a unique pattern in that its highest concentration is attained at the hematite lutite transitions (similarly with the REE in this regard), while very low and seemingly invariant concentration is exhibited within the core of the main orebodies. The same pattern seems to be reproduced to a degree by the corresponding bulk MgO component, whereby MgO abundance maxima are associated with the basal hematite lutite and the hematitic flanks of the Mn-ore zone, while the core of the Mn-rich layer attains relatively low and essentially invariant MgO concentrations. This implicates a close and direct association of Co with the hematite fraction of the rocks and a concurrent enrichment in Mn-rich carbonate (dolomite). On the other hand, Mo seems to have a direct and clear association with peak MnO2 content of the rocks, which in turn presents a high possibility of Mo having adsorbed onto primary Mn-oxyhydroxides in the water column, thus providing evidence that Mn-oxide must have acted as an important Mo sink, at least locally. Finally, the carbonate-carbon isotope results provide a useful tool that brings the two stratigraphic sections “together“, in conjunction with other correlatable chemostratigraphic parameters (e.g. Co, Mg). The results demonstrate that bulk carbon fluxes and isotopic signals in the sediments must reflect primary processes of deposition, and that correlation across two apparently disparate lithostratigraphic sections can be effected. The key finding is that, at times, manganese deposition in one part of a vii stratified basin was evidently accompanied by simultaneous BIF deposition at another, thus painting a very complex picture of massive primary chemical precipitation of Fe and Mn at the dawn of the GOE. , Thesis (MSc) -- Faculty of Science, Geology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Masoabi, Ntseka Thomas
- Date: 2022-10-14
- Subjects: Chemostratigraphy , Great Oxygenation Event , Manganese ores Geology South Africa Northern Cape , Banded iron formation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362938 , vital:65376
- Description: The giant Kalahari Manganese Field (KMF), located in the Northern Cape Province, South Africa, comprises approximately half of the world’s manganese resources, estimated at about eight billion tons at grades ranging from 20-48 wt%. The KMF is linked to a period in geological time when the Earth’s atmospheric and oceanic conditions underwent a major transition from oxygen-deficient to oxygen-enriched conditions – an event famously referred to as the Great Oxidation Event (GOE) that occurred around 2.4 Ga. The KMF deposits are hosted in Banded Iron Formation (BIF) of the Paleoproterozoic Hotazel Formation in the uppermost Transvaal Supergroup. The sedimentary Mn ores are interbedded with Hotazel BIF in the form of three alternating depositional cycles of BIF, transitional hematite lutite and laminated, carbonate-rich manganese ore. The lowermost and thickest of the three cycles is the most economically significant and has been mined for several decades on a large scale from the southernmost KMF. In this study, two drill cores from the southern KMF were inspected, logged and sampled at a high resolution of approximately half-meter interval per sample. The selected cores, namely G774, capturing the lower portion of the Hotazel Formation from the Mamatwan locality, and MP-56, capturing the corresponding portion from the Middleplaats locality, are geographically proximal to each other, with a horizontal distance of roughly 3 km separating the two of them. The G774 drill core is characterized by a conspicuously thick manganese layer covering a thickness of 50 m, with the overlying BIF reaching a total thickness of 11 m. The MP-56 drill core, on the other hand, has a relatively thinner corresponding manganese layer of 30 m in thickness, while the overlying BIF layer exhibits a thickness of 24 m. The extent of sampling up-section was constrained by an apparently coeval black shale layer which represents the chosen upper stratigraphic marker for the lower part of the Hotazel section in the broader area that is under investigation in this thesis. That way, a high resolution chemostratigraphic approach was employed to elucidate the potential factors contributing to the relative sedimentary lateral thickness variations seen across the southernmost KMF. High-resolution geochemical data were used to explore relationships and signals that might constrain relative precipitation rates for iron and manganese against detrital species, fluctuating redox conditions in the original environment of deposition, and chemostratigraphic correlation. All geochemical data (i.e., major oxides, minor and trace elements and carbonate carbon isotopes) were obtained respectively through employing X-ray Fluorescence (XRF), Laser Ablation Inductively Coupled Mass Spectrometry (ICP-MS), and Gas-source mass spectrometry. Comparative considerations made between the bulk geochemistry of the two sequences (i.e., Mamatwan and Middleplaats sections) reveal that periods of high-Mn deposition in the Hotazel Formation appear to be very Ca-carbonate rich (as indicated by high CaO, LOI and Sr concentrations). This, in turn, suggests that the Mn abundance is in the Hotazel ores is controlled mainly by the silicate phase braunite and is diluted by the deposition of Ca-carbonate through time. Bulk-rock concentration results for trace elements of the High Field Strength Element (HFSE) group (namely Zr, Hf, Y, Nb and Sc) were utilized to constrain the rates of either clastic and/or volcanic detrital inputs, as they traditionally represent refractory mineral particles of a common detrital/volcanic origin. The two chemosedimentary sequences preserve these elements in very low and thus quantitatively negligible concentrations – suggesting that the Hotazel depositional environment received very low and insignificant influx of a terrigeneous detrital component. A selection of these elements was therefore used to deduce, with caution, the relative as opposed to absolute precipitation rate of the major chemical constituents (i.e., Fe + Si vs Mn + carbonate), assuming a constant detrital flux through time. It was found that the relative abundances of Zr, Y and Nb is roughly 1.5 – 2 times as high in the BIF lithofacies relative to the Mn ones at both localities. This led to the inference that the Mn-enriched portion of the sediment must have been deposited at approximately twice the rate that the Fe-rich (BIF) portion was originally deposited. In terms of redox-sensitive elements, the elements Co and Mo seem to reveal the most valuable insights into the redox environment of primary chemical deposition. Cobalt displays a unique pattern in that its highest concentration is attained at the hematite lutite transitions (similarly with the REE in this regard), while very low and seemingly invariant concentration is exhibited within the core of the main orebodies. The same pattern seems to be reproduced to a degree by the corresponding bulk MgO component, whereby MgO abundance maxima are associated with the basal hematite lutite and the hematitic flanks of the Mn-ore zone, while the core of the Mn-rich layer attains relatively low and essentially invariant MgO concentrations. This implicates a close and direct association of Co with the hematite fraction of the rocks and a concurrent enrichment in Mn-rich carbonate (dolomite). On the other hand, Mo seems to have a direct and clear association with peak MnO2 content of the rocks, which in turn presents a high possibility of Mo having adsorbed onto primary Mn-oxyhydroxides in the water column, thus providing evidence that Mn-oxide must have acted as an important Mo sink, at least locally. Finally, the carbonate-carbon isotope results provide a useful tool that brings the two stratigraphic sections “together“, in conjunction with other correlatable chemostratigraphic parameters (e.g. Co, Mg). The results demonstrate that bulk carbon fluxes and isotopic signals in the sediments must reflect primary processes of deposition, and that correlation across two apparently disparate lithostratigraphic sections can be effected. The key finding is that, at times, manganese deposition in one part of a vii stratified basin was evidently accompanied by simultaneous BIF deposition at another, thus painting a very complex picture of massive primary chemical precipitation of Fe and Mn at the dawn of the GOE. , Thesis (MSc) -- Faculty of Science, Geology, 2022
- Full Text:
- Date Issued: 2022-10-14
Conceptualising mental distress from an African psychology paradigm: using an interpretative phenomenological analysis of the views of traditional healers
- Nabo-Bazana, Sandisiwe Sifanelwe
- Authors: Nabo-Bazana, Sandisiwe Sifanelwe
- Date: 2022-10-14
- Subjects: Healers South Africa , Traditional healer , Mental distress , Black psychology , Afrocentrism
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406213 , vital:70249
- Description: With South Africa's long history of colonialism and racial oppression, there are still services in the country that many South Africans cannot relate to, including psychology. Research shows that many South Africans experience and are affected by mental distress due to several factors, including poverty, unemployment, and traumatic experiences. Managing and treating such distress has always been challenging for most South Africans. Some debates question the relevance of psychological services from the West in a South African context. This study explores other approaches to psychology that look beyond the Biopsychosocial model when dealing with certain types of disorders in an African context. African psychology, or the Afrocentric approach, looks at what is beneath the surface, not just the presenting problem. Mainstream psychology strives to be universal and applicable to all. However, African psychology disagrees with this notion. African psychology perceives human beings as strongly influenced by social and cultural influences. The focus of this approach includes the spiritual realm and the attached meanings. There is evidence for the need to merge Traditional and Western medicine. The research methodology for this study is qualitative, using Interpretative Phenomenological Analysis. IPA allows for a critical engagement with the ways in which participants construct their reality. The researcher carried out semi-structured interviews to enable participants, all traditional healers (3 female and 2 male), to narrate their experiences dealing with mentally distressed clients. The accounts of these traditional healers were analysed focusing on people who have experienced mental distress. From the analysis and synthesis of the themes, findings illustrate how traditional healers conceptualise and construct mental distress from an African Psychology paradigm. An emerging core theme was the importance of the divine call and its influence on the chosen treatments. More studies are needed to illustrate the potential for collaboration between African Traditional healing and EuroAmerican healing practices, to provide holistic services to people in need. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Nabo-Bazana, Sandisiwe Sifanelwe
- Date: 2022-10-14
- Subjects: Healers South Africa , Traditional healer , Mental distress , Black psychology , Afrocentrism
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406213 , vital:70249
- Description: With South Africa's long history of colonialism and racial oppression, there are still services in the country that many South Africans cannot relate to, including psychology. Research shows that many South Africans experience and are affected by mental distress due to several factors, including poverty, unemployment, and traumatic experiences. Managing and treating such distress has always been challenging for most South Africans. Some debates question the relevance of psychological services from the West in a South African context. This study explores other approaches to psychology that look beyond the Biopsychosocial model when dealing with certain types of disorders in an African context. African psychology, or the Afrocentric approach, looks at what is beneath the surface, not just the presenting problem. Mainstream psychology strives to be universal and applicable to all. However, African psychology disagrees with this notion. African psychology perceives human beings as strongly influenced by social and cultural influences. The focus of this approach includes the spiritual realm and the attached meanings. There is evidence for the need to merge Traditional and Western medicine. The research methodology for this study is qualitative, using Interpretative Phenomenological Analysis. IPA allows for a critical engagement with the ways in which participants construct their reality. The researcher carried out semi-structured interviews to enable participants, all traditional healers (3 female and 2 male), to narrate their experiences dealing with mentally distressed clients. The accounts of these traditional healers were analysed focusing on people who have experienced mental distress. From the analysis and synthesis of the themes, findings illustrate how traditional healers conceptualise and construct mental distress from an African Psychology paradigm. An emerging core theme was the importance of the divine call and its influence on the chosen treatments. More studies are needed to illustrate the potential for collaboration between African Traditional healing and EuroAmerican healing practices, to provide holistic services to people in need. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
Constraints on Cr-PGE Mineralisation Models: Geochemical and petrological studies in the Middle Group 1 and 3 Chromitites, Western Limb, Bushveld Complex, South Africa
- Authors: Arunachellan, Yogendran
- Date: 2022-10-14
- Subjects: Chromite South Africa Bushveld Complex , Mineralogy South Africa Bushveld Complex , Geochemical surveys South Africa Bushveld Complex
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362730 , vital:65357
- Description: The Bushveld Complex in South Africa has been of interest in various research groups for decades, along with diverse theories regarding its origin, formation, and emplacement. These theories include magma mixing, contamination, and changes in the chamber's ephemeral parameters. Of interest for our current study is the formation of the middle group chromitite layers in the Western Limb. In this research, we aimed to determine the emplacement mechanism of the MG group chromitites by scrutinising the MG 1 and MG 3 layers. In core KD 151, the focus was placed on the MG 3 and MG 1 chromitite layers and their associated silicate rocks; on these regions of the core, time was spent for detailed observations. The differences and similarities of these layers were explored as the study advanced. The objectives were to determine the in-situ or proximal crystallisation of the chromitite by evaluating mineral textures and compositions. This required that we determine the characteristics of the immediate HW (hanging wall) and FW (footwall) to these chromitites, with insights into the relationship that anorthositic zones may offer and examine the PGE profiles of the chromitites in contrasting lithological settings. The sampled borehole was in the Western Limb of the Bushveld Complex; the immediate HW, chromitite layers and FW were divided into sections (2.5 x 5 cm) along selected horizons for a microscale study. The preliminary results of a study on the sub-economic Middle Group (MG) layers within the Critical Zone (CZ), contrasting the MG 1 (Lower CZ) and MG 3 (Upper CZ) chromitite layers of the Rustenburg Layered Suite of the Bushveld Complex, South Africa were analysed. The MG 3 and 1 suites of silicate rocks show disequilibrium textures between the pyroxenes and plagioclase, forming discontinuous olivine rims. These reaction rims are interpreted as products of magmatic aqueous fluid-facilitated reactions with minerals in a sub-solidus state. Deformation of the plagioclase was also noted in MG 1; this fracturing indicates either the transportation of these minerals or compaction by an overlying crystal mush. The MG 3 package of plagioclase, pyroxene and chromite compositions range from An67-78, En71-86 and Cr# of 68-84, respectively. The MG 1 package of plagioclase, pyroxene and chromite compositions are An64-91, En79-88 and Cr# of 70-80, respectively. The Cu/Pd ratio decreases from the base of the FW as it approaches the base of the chromitite, then remains low within the chromitite layer and finally increases upwards in the HW. These trends are observed in both the MG 3 and 1 package, therefore not influenced by the sulphide control. There is a decrease in both IPGE and PPGE upwards as the HW progresses into the chromitite. The FW levels of Ir and Ru increase upwards immediately adjacent to the FW contact, while Pd and Rh concentrations decrease. The whole-rock Mg# indicates a decrease in the MG 3 with a uniform increase in the HW and an erratic upward increase observed in the FW. The chondrite normalised PGE plots show a bell-shaped curve which is evident for the En content of the pyroxenes and the content of plagioclase with the highest values in the chromitite layer itself. The Cr# of the chromite decreases upwards with the highest values along the FW. The Cu/Pd ratio for MG 1 indicates chromitite control rather than sulphide control of the PGE. The whole-rock Mg# decreases in MG 1 with higher uniform values observed in the HW and FW. The geochemical and petrological data from the MG group study revealed that in situ fractional crystallisation seems unlikely as the sole mechanism for their formation in the CZ. A model is suggested in which the migration and transport of a magmatic slurry type suspension with accompanying hydrous fluids would likely have resulted in the emplacement of these chromitite packages along with the PGE mineralisation of the chromitites. , Thesis (MSc) -- Faculty of Science, Geology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Arunachellan, Yogendran
- Date: 2022-10-14
- Subjects: Chromite South Africa Bushveld Complex , Mineralogy South Africa Bushveld Complex , Geochemical surveys South Africa Bushveld Complex
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362730 , vital:65357
- Description: The Bushveld Complex in South Africa has been of interest in various research groups for decades, along with diverse theories regarding its origin, formation, and emplacement. These theories include magma mixing, contamination, and changes in the chamber's ephemeral parameters. Of interest for our current study is the formation of the middle group chromitite layers in the Western Limb. In this research, we aimed to determine the emplacement mechanism of the MG group chromitites by scrutinising the MG 1 and MG 3 layers. In core KD 151, the focus was placed on the MG 3 and MG 1 chromitite layers and their associated silicate rocks; on these regions of the core, time was spent for detailed observations. The differences and similarities of these layers were explored as the study advanced. The objectives were to determine the in-situ or proximal crystallisation of the chromitite by evaluating mineral textures and compositions. This required that we determine the characteristics of the immediate HW (hanging wall) and FW (footwall) to these chromitites, with insights into the relationship that anorthositic zones may offer and examine the PGE profiles of the chromitites in contrasting lithological settings. The sampled borehole was in the Western Limb of the Bushveld Complex; the immediate HW, chromitite layers and FW were divided into sections (2.5 x 5 cm) along selected horizons for a microscale study. The preliminary results of a study on the sub-economic Middle Group (MG) layers within the Critical Zone (CZ), contrasting the MG 1 (Lower CZ) and MG 3 (Upper CZ) chromitite layers of the Rustenburg Layered Suite of the Bushveld Complex, South Africa were analysed. The MG 3 and 1 suites of silicate rocks show disequilibrium textures between the pyroxenes and plagioclase, forming discontinuous olivine rims. These reaction rims are interpreted as products of magmatic aqueous fluid-facilitated reactions with minerals in a sub-solidus state. Deformation of the plagioclase was also noted in MG 1; this fracturing indicates either the transportation of these minerals or compaction by an overlying crystal mush. The MG 3 package of plagioclase, pyroxene and chromite compositions range from An67-78, En71-86 and Cr# of 68-84, respectively. The MG 1 package of plagioclase, pyroxene and chromite compositions are An64-91, En79-88 and Cr# of 70-80, respectively. The Cu/Pd ratio decreases from the base of the FW as it approaches the base of the chromitite, then remains low within the chromitite layer and finally increases upwards in the HW. These trends are observed in both the MG 3 and 1 package, therefore not influenced by the sulphide control. There is a decrease in both IPGE and PPGE upwards as the HW progresses into the chromitite. The FW levels of Ir and Ru increase upwards immediately adjacent to the FW contact, while Pd and Rh concentrations decrease. The whole-rock Mg# indicates a decrease in the MG 3 with a uniform increase in the HW and an erratic upward increase observed in the FW. The chondrite normalised PGE plots show a bell-shaped curve which is evident for the En content of the pyroxenes and the content of plagioclase with the highest values in the chromitite layer itself. The Cr# of the chromite decreases upwards with the highest values along the FW. The Cu/Pd ratio for MG 1 indicates chromitite control rather than sulphide control of the PGE. The whole-rock Mg# decreases in MG 1 with higher uniform values observed in the HW and FW. The geochemical and petrological data from the MG group study revealed that in situ fractional crystallisation seems unlikely as the sole mechanism for their formation in the CZ. A model is suggested in which the migration and transport of a magmatic slurry type suspension with accompanying hydrous fluids would likely have resulted in the emplacement of these chromitite packages along with the PGE mineralisation of the chromitites. , Thesis (MSc) -- Faculty of Science, Geology, 2022
- Full Text:
- Date Issued: 2022-10-14
Contextualising job satisfaction amongst lowest paid support staff in a Higher Education institution
- Madito, Gotlannamang Moloiesi Boipelo
- Authors: Madito, Gotlannamang Moloiesi Boipelo
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/405984 , vital:70225
- Description: Thesis embargoed. To be relased in 2024. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
Contextualising job satisfaction amongst lowest paid support staff in a Higher Education institution
- Authors: Madito, Gotlannamang Moloiesi Boipelo
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/405984 , vital:70225
- Description: Thesis embargoed. To be relased in 2024. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
Cyborg bodies and the search for self: an ethnographic exploration of supportive technologies as tools to mitigate daily distresses
- Authors: Kibane, Lebogang Zandile
- Date: 2022-10-14
- Subjects: Posthuman , Posthumanism , Anthropology , m-health , Wireless communication systems in medical care , Cyber-ethnography , Distress (Psychology)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408625 , vital:70510
- Description: This dissertation explores the ways in which individuals embody cyborg realities through engaging with technological aids. It examines how these interactions have the potential to bring to light new ways of thinking about and experiencing daily distresses. Pertinent human technology interaction was observed through an overarching cyborg lens, embedded in posthumanist thought. The study began with a “human as cyborg” ontology that sought to impress upon readers the intimacy with which technology is entangled in our lives. It aimed to call to attention the use of the cyborg myth as it is concerned with “transgressed boundaries, potent fusions, and dangerous possibilities” (Haraway, 1985:7). The study followed a cyber ethnographic strategy, where cyber ethnography refers to a virtual research method that observes social and cultural phenomena that are mediated by online interactions. Data was collected over a period of eight months, beginning in December 2020 and ending in July 2021. It was collected through the following means: a short self-administered online questionnaire, computer mediated in-depth interviews, and group interviews. I also kept a journal on reflections of my own use of these supportive technologies. After analysing the results of in-depth interviews with nine primary participants and twenty-six online questionnaires, the following key themes were brought to light: Firstly, the tethered self, or cyborg self, engages in evolved acts of ‘care of the self’ mitigated through supportive technology use. Secondly, access to new technologies brings about new ways of performing the self. Thirdly, engagement with supportive technologies provides opportunity for aiding distress in the way of encouraging self-reflective and self-interrogation behaviours as seen in mobile health app use. This constant self-interrogation behaviour in turn develops a kind of technological dependency, characterized by escapism and evolved methods of self- soothing. Lastly, exploring the use of technology to aid distress revealed that technologies generate equal opportunities for improved well-being, as they do for a decreased sense of connectedness and security. The research demonstrates that supportive technology use is entangled in the fabric of our everyday lives. Through it we fashion our identities, alleviate distress, evade distress, and discover new causes of distress. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Kibane, Lebogang Zandile
- Date: 2022-10-14
- Subjects: Posthuman , Posthumanism , Anthropology , m-health , Wireless communication systems in medical care , Cyber-ethnography , Distress (Psychology)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408625 , vital:70510
- Description: This dissertation explores the ways in which individuals embody cyborg realities through engaging with technological aids. It examines how these interactions have the potential to bring to light new ways of thinking about and experiencing daily distresses. Pertinent human technology interaction was observed through an overarching cyborg lens, embedded in posthumanist thought. The study began with a “human as cyborg” ontology that sought to impress upon readers the intimacy with which technology is entangled in our lives. It aimed to call to attention the use of the cyborg myth as it is concerned with “transgressed boundaries, potent fusions, and dangerous possibilities” (Haraway, 1985:7). The study followed a cyber ethnographic strategy, where cyber ethnography refers to a virtual research method that observes social and cultural phenomena that are mediated by online interactions. Data was collected over a period of eight months, beginning in December 2020 and ending in July 2021. It was collected through the following means: a short self-administered online questionnaire, computer mediated in-depth interviews, and group interviews. I also kept a journal on reflections of my own use of these supportive technologies. After analysing the results of in-depth interviews with nine primary participants and twenty-six online questionnaires, the following key themes were brought to light: Firstly, the tethered self, or cyborg self, engages in evolved acts of ‘care of the self’ mitigated through supportive technology use. Secondly, access to new technologies brings about new ways of performing the self. Thirdly, engagement with supportive technologies provides opportunity for aiding distress in the way of encouraging self-reflective and self-interrogation behaviours as seen in mobile health app use. This constant self-interrogation behaviour in turn develops a kind of technological dependency, characterized by escapism and evolved methods of self- soothing. Lastly, exploring the use of technology to aid distress revealed that technologies generate equal opportunities for improved well-being, as they do for a decreased sense of connectedness and security. The research demonstrates that supportive technology use is entangled in the fabric of our everyday lives. Through it we fashion our identities, alleviate distress, evade distress, and discover new causes of distress. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
- Date Issued: 2022-10-14
Design, synthesis, manufacture, characterization and evaluation of lipid nanocapsules in chitosan-iota-carrageenan based hydrogel scaffold as a potential anti-Covid-19 drug delivery system
- Authors: Mukubwa, Grady Kathondo
- Date: 2022-10-14
- Subjects: Nanocapsules Design , Hydrogel , COVID-19 (Disease) , Characterization , Drug delivery systems
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364955 , vital:65665
- Description: Covid-19 is a deadly viral disease that has been rampant around the world since 2019. Although the successful introduction of the vaccine has reduced the spread of covid-19, new cases and deaths are still being recorded. To date, no specific curative antiviral treatment has been approved for covid-19. However, many existing antiviral drugs have been and are still being studied against covid-19 and some of them, such as Remdesivir, have shown promise and could be repurposed to treat this infection. Unfortunately, antiviral drugs are prone to resistance as most of them have poor biopharmaceutical properties, including low solubility, permeability and bioavailability, which could hinder any clinical success. Recent advances in nanotechnology-based delivery systems have made it possible to improve the biopharmaceutical properties of many drugs, especially those of poorly water-soluble drugs, by formulating them as lipid nanoparticles (LNP). Thus, in order to contribute to the fight against covid-19, this work aimed to develop Lipid Nanocapsules (LNC), based on some natural raw materials, which could improve the biopharmaceutical properties of antiviral drugs. In addition, since covid-19 infection is mainly respiratory, this work also aimed to fabricate a targeted delivery system based on a hydrogel capable of entrapping LNC and ensuring their efficient deposition and release in the lungs. The LNC consisted of a mixture of medium-chain triglycerides oil (MCT oil), crude soy lecithin, tween 80, NaCl and water, while the hydrogel consisted of a chitosan-grafted-iota carrageenan-grafted-poly (acrylamide-co-acrylic acid) system (CS-iCar-p (AAm-Co-AA)). Efavirenz (EFV), a drug with very low water solubility that has recently been demonstrated to have the potential to influence sars-cov-2 life cycle through different targets (3CLP, RdRp, Hellicase, 3’to5’exonuclease, 2’-O-ribose methyltransferase and EndoRNAse), was chosen as the model drug to evaluate the developed delivery system. The combination of LNP and hydrogel results in a delivery system known as the LNP-hydrogel composite, an emerging area of research in the field of drug delivery. To date, no research has reported the design and fabrication of an LNC-CS-iCar-p (AAm-Co-AA) hydrogel composite that could effectively deliver an antiviral drug to the lungs in addition to its advantages in terms of biological activities. Prior to the design of experiment, EFV solubility was assessed in water, labrafac lipophile 1349 and MCT oil. After that, the Design Expert Software version 13 was used to design the different experiments performed in this work. The I-optimal mixture design of experiments was performed for both LNC preparation and CS-iCar-p (AAm-Co-AA) hydrogel synthesis to study the impact of raw materials on the characteristics of these delivery systems. LNC were prepared using the phase inversion method while the free radical precipitation graft copolymerization method was used to synthesize hydrogel. In order to build polynomial models that could predict the amount of drug both LNC and CS-iCar-p (AAm-Co-AA) hydrogel can entrap, a D-optimal (custom) randomized design was performed. Moreover, various characterization techniques were used to investigate the physicochemical properties of the developed delivery systems. Thereafter, drug release studies were performed using a 1% sodium lauryl sulfate solution adjusted to either pH 4 or 7. Solubility studies revealed that EFV was more soluble in labrafac lipophile 1349 and in MCT oil than in water; therefore, given its affordability, MCT oil was used for the LNC formulation. The design of experiment carried out allowed the construction of polynomial models that could predict, on the one hand, the droplet size, the polydispersity index and the Zeta potential of LNC, which were respectively around 50nm, below 0.2 and below -33. On the other hand, the model could predict the swelling capacity of the synthesized hydrogel, which was optimised to about 30,000% (300 g of water to 1 g of hydrogel). This turned out to be influenced by the proportion of polymers, the ratio of monomers as well as the concentration of the cross-linking agent. In addition, the characterization techniques further supported the improvement of EFV solubility by highlighting its conversion into its amorphous state after encapsulation in LNC. They also confirmed successful synthesis of CS-iCar-p (AAm-co-AA) hydrogel. LNC were able to encapsulate about 87% of EFV while the synthesized CS-iCar-p (AAm-co-AA) hydrogel entrapped around 53% of EFV encapsulated in LNC. While LNC were able to release 42% and 27% of EFV after 74 hours in a 1% sodium lauryl sulfate solution (SLS) at pH 7 and pH 4 respectively, the LNC-CS-iCar-p (AAm-co-AA) hydrogel composite released about 50% and 40% of the drug after 9 days in the same release medium. Interestingly, the chemical integrity of the drug was preserved throughout the manufacturing process up to after its release, suggesting that the developed LNC-CS-iCar-p (AAm-co-AA) hydrogel composite could be used as a novel potential anticovid-19 drugs delivery system. , Thesis (MSc) -- Faculty of Science, Chemistry, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Mukubwa, Grady Kathondo
- Date: 2022-10-14
- Subjects: Nanocapsules Design , Hydrogel , COVID-19 (Disease) , Characterization , Drug delivery systems
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364955 , vital:65665
- Description: Covid-19 is a deadly viral disease that has been rampant around the world since 2019. Although the successful introduction of the vaccine has reduced the spread of covid-19, new cases and deaths are still being recorded. To date, no specific curative antiviral treatment has been approved for covid-19. However, many existing antiviral drugs have been and are still being studied against covid-19 and some of them, such as Remdesivir, have shown promise and could be repurposed to treat this infection. Unfortunately, antiviral drugs are prone to resistance as most of them have poor biopharmaceutical properties, including low solubility, permeability and bioavailability, which could hinder any clinical success. Recent advances in nanotechnology-based delivery systems have made it possible to improve the biopharmaceutical properties of many drugs, especially those of poorly water-soluble drugs, by formulating them as lipid nanoparticles (LNP). Thus, in order to contribute to the fight against covid-19, this work aimed to develop Lipid Nanocapsules (LNC), based on some natural raw materials, which could improve the biopharmaceutical properties of antiviral drugs. In addition, since covid-19 infection is mainly respiratory, this work also aimed to fabricate a targeted delivery system based on a hydrogel capable of entrapping LNC and ensuring their efficient deposition and release in the lungs. The LNC consisted of a mixture of medium-chain triglycerides oil (MCT oil), crude soy lecithin, tween 80, NaCl and water, while the hydrogel consisted of a chitosan-grafted-iota carrageenan-grafted-poly (acrylamide-co-acrylic acid) system (CS-iCar-p (AAm-Co-AA)). Efavirenz (EFV), a drug with very low water solubility that has recently been demonstrated to have the potential to influence sars-cov-2 life cycle through different targets (3CLP, RdRp, Hellicase, 3’to5’exonuclease, 2’-O-ribose methyltransferase and EndoRNAse), was chosen as the model drug to evaluate the developed delivery system. The combination of LNP and hydrogel results in a delivery system known as the LNP-hydrogel composite, an emerging area of research in the field of drug delivery. To date, no research has reported the design and fabrication of an LNC-CS-iCar-p (AAm-Co-AA) hydrogel composite that could effectively deliver an antiviral drug to the lungs in addition to its advantages in terms of biological activities. Prior to the design of experiment, EFV solubility was assessed in water, labrafac lipophile 1349 and MCT oil. After that, the Design Expert Software version 13 was used to design the different experiments performed in this work. The I-optimal mixture design of experiments was performed for both LNC preparation and CS-iCar-p (AAm-Co-AA) hydrogel synthesis to study the impact of raw materials on the characteristics of these delivery systems. LNC were prepared using the phase inversion method while the free radical precipitation graft copolymerization method was used to synthesize hydrogel. In order to build polynomial models that could predict the amount of drug both LNC and CS-iCar-p (AAm-Co-AA) hydrogel can entrap, a D-optimal (custom) randomized design was performed. Moreover, various characterization techniques were used to investigate the physicochemical properties of the developed delivery systems. Thereafter, drug release studies were performed using a 1% sodium lauryl sulfate solution adjusted to either pH 4 or 7. Solubility studies revealed that EFV was more soluble in labrafac lipophile 1349 and in MCT oil than in water; therefore, given its affordability, MCT oil was used for the LNC formulation. The design of experiment carried out allowed the construction of polynomial models that could predict, on the one hand, the droplet size, the polydispersity index and the Zeta potential of LNC, which were respectively around 50nm, below 0.2 and below -33. On the other hand, the model could predict the swelling capacity of the synthesized hydrogel, which was optimised to about 30,000% (300 g of water to 1 g of hydrogel). This turned out to be influenced by the proportion of polymers, the ratio of monomers as well as the concentration of the cross-linking agent. In addition, the characterization techniques further supported the improvement of EFV solubility by highlighting its conversion into its amorphous state after encapsulation in LNC. They also confirmed successful synthesis of CS-iCar-p (AAm-co-AA) hydrogel. LNC were able to encapsulate about 87% of EFV while the synthesized CS-iCar-p (AAm-co-AA) hydrogel entrapped around 53% of EFV encapsulated in LNC. While LNC were able to release 42% and 27% of EFV after 74 hours in a 1% sodium lauryl sulfate solution (SLS) at pH 7 and pH 4 respectively, the LNC-CS-iCar-p (AAm-co-AA) hydrogel composite released about 50% and 40% of the drug after 9 days in the same release medium. Interestingly, the chemical integrity of the drug was preserved throughout the manufacturing process up to after its release, suggesting that the developed LNC-CS-iCar-p (AAm-co-AA) hydrogel composite could be used as a novel potential anticovid-19 drugs delivery system. , Thesis (MSc) -- Faculty of Science, Chemistry, 2022
- Full Text:
- Date Issued: 2022-10-14
Development and optimisation of a qPCR assay for the enumeration of Cryptophlebia leucotreta granulovirus (CrleGV) used for commercial applications
- Authors: Mela, Thuthula
- Date: 2022-10-14
- Subjects: Cryptophlebia leucotreta granulovirus , Cryptophlebia leucotreta , Late expression factor 8 (LEF-8) , Late expression factor 9 , Dark field microscopy , Genomic DNA , Polymerase chain reaction , Plasmids
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362949 , vital:65377
- Description: The citrus industry contributes significantly to the South African agricultural sector. Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is highly important to the South African citrus industry as it is classified as a phytosanitary pest by most international markets. Thaumatotibia leucotreta has caused an estimated annual loss of up to R100 million to the industry. In order to control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been used. One of the components of this programme is Cryptophlebia leucotreta granulovirus (CrleGV), which has been formulated to a registered biopesticide namely Cryptogran and has been successfully applied in the field for over 15 years. To use CrleGV as biopesticides, quantification of the viral particles is required to perform bioassays for field trials and formulation, among other applications. Darkfield microscopy is a traditional method used for the quantification of CrleGV; however, the method is characterised as being subjective, tedious, labour intensive, and time-consuming. This study aims to develop and optimise a qPCR technique to accurately quantify CrleGV-SA OBs using plasmid DNA for downstream applications. Firstly, lef-8, lef-9, and granulin conserved genes from CrleGV-SA and CrleGV-CV3 genome sequences were analysed by performing multiple alignments to evaluate the degree of identity between these genes. This was done to design two sets of oligonucleotides (internal and external) from regions with the highest identity. Subsequently, in silico testing was done to evaluate the designed oligonucleotides to determine whether they specifically bind to the selected target regions. Secondly, three sets of DNA plasmids (pJET1.2-Gran, pJET1.2-lef-9, and pJET1.2-lef-8) were constructed, each containing a target region for either granulin, lef-9, and lef-8 genes for use as standards in a downstream qPCR assay. This was achieved by first extracting gDNA from CrleGV-SA OBs and using the gDNA as a template to PCR amplify the target regions of the selected gene regions with the designed oligonucleotides. Subsequently, the PCR amplified regions were then directly ligated into the pJET1.2/blunt vector, and the plasmids were confirmed by colony PCR, restriction enzyme digestion, and Sanger sequencing. Thirdly, two different methods of CrleGV-SA gDNA extraction were compared to determine which method has the best yields in terms of concentration. The extraction methods compared were the Quick-DNA Miniprep Plus kit according to manufacturer’s instructions (Method 1a), pre-treatment with Na2CO3 prior to using the Quick-DNA Miniprep Plus kit (Method 1b), pre- treatment with Na2CO3, and neutralisation with Tris-HCl prior to gDNA extraction using the Quick-DNA Miniprep Plus kit (Method 1c) and the CTAB method (Method 2). The gDNA concentration and purity for all samples were determined using a Nanodrop spectrophotometer. Method 1c (Na2CO3 and Tris-HCl pre-treated plus Quick-DNA Miniprep Plus kit) was the most efficient at extracting genomic DNA compared with the other methods, resulting in the highest DNA concentration in short processing time. Fourthly, plasmid standards were evaluated for use in the qPCR assay. This was done as it was important to consider the efficacy of the oligonucleotides; including the ability of the oligonucleotides to anneal to the appropriate segment of DNA without extensive formation of oligonucleotides dimers, non-specific annealing, or formation of secondary structure. In addition, it was done to ensure that highly accurate standard curves were generated. The standard curves were to be utilised in the downstream qPCR assay to determine the quantity of test samples by interpolation, reading from the values within the standard curve. Lastly, darkfield microscopy and qPCR methods of enumeration were compared to verify their accuracy and determine the most consistent and comparable method. This was achieved by quantifying the purified, crude-purified, and viral formulated CrleGV-SA suspensions using these methods. Subsequently, a statistical analysis was conducted to compare the results produced by the two enumeration methods. The obtained results showed that the granulin, lef- 8 and lef-9 qPCR values did not significantly differ from the darkfield microscopy results. The findings of this study revealed that the two assays, lef-8 qPCR and lef-9 qPCR, were more robust, sensitive, and efficient for the quantification of CrleGV-SA. Thus, this study has successfully developed a qPCR assay that is comparable with the traditional darkfield microscopy counting technique. This is the first study to use the qPCR technique to enumerate CrleGV-SA using plasmid standards. The developed qPCR assay is reliable, rapid, and cost- effective and has a great potential to be used as an alternative method to darkfield microscopy in the laboratory and commercial settings. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Mela, Thuthula
- Date: 2022-10-14
- Subjects: Cryptophlebia leucotreta granulovirus , Cryptophlebia leucotreta , Late expression factor 8 (LEF-8) , Late expression factor 9 , Dark field microscopy , Genomic DNA , Polymerase chain reaction , Plasmids
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362949 , vital:65377
- Description: The citrus industry contributes significantly to the South African agricultural sector. Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is highly important to the South African citrus industry as it is classified as a phytosanitary pest by most international markets. Thaumatotibia leucotreta has caused an estimated annual loss of up to R100 million to the industry. In order to control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been used. One of the components of this programme is Cryptophlebia leucotreta granulovirus (CrleGV), which has been formulated to a registered biopesticide namely Cryptogran and has been successfully applied in the field for over 15 years. To use CrleGV as biopesticides, quantification of the viral particles is required to perform bioassays for field trials and formulation, among other applications. Darkfield microscopy is a traditional method used for the quantification of CrleGV; however, the method is characterised as being subjective, tedious, labour intensive, and time-consuming. This study aims to develop and optimise a qPCR technique to accurately quantify CrleGV-SA OBs using plasmid DNA for downstream applications. Firstly, lef-8, lef-9, and granulin conserved genes from CrleGV-SA and CrleGV-CV3 genome sequences were analysed by performing multiple alignments to evaluate the degree of identity between these genes. This was done to design two sets of oligonucleotides (internal and external) from regions with the highest identity. Subsequently, in silico testing was done to evaluate the designed oligonucleotides to determine whether they specifically bind to the selected target regions. Secondly, three sets of DNA plasmids (pJET1.2-Gran, pJET1.2-lef-9, and pJET1.2-lef-8) were constructed, each containing a target region for either granulin, lef-9, and lef-8 genes for use as standards in a downstream qPCR assay. This was achieved by first extracting gDNA from CrleGV-SA OBs and using the gDNA as a template to PCR amplify the target regions of the selected gene regions with the designed oligonucleotides. Subsequently, the PCR amplified regions were then directly ligated into the pJET1.2/blunt vector, and the plasmids were confirmed by colony PCR, restriction enzyme digestion, and Sanger sequencing. Thirdly, two different methods of CrleGV-SA gDNA extraction were compared to determine which method has the best yields in terms of concentration. The extraction methods compared were the Quick-DNA Miniprep Plus kit according to manufacturer’s instructions (Method 1a), pre-treatment with Na2CO3 prior to using the Quick-DNA Miniprep Plus kit (Method 1b), pre- treatment with Na2CO3, and neutralisation with Tris-HCl prior to gDNA extraction using the Quick-DNA Miniprep Plus kit (Method 1c) and the CTAB method (Method 2). The gDNA concentration and purity for all samples were determined using a Nanodrop spectrophotometer. Method 1c (Na2CO3 and Tris-HCl pre-treated plus Quick-DNA Miniprep Plus kit) was the most efficient at extracting genomic DNA compared with the other methods, resulting in the highest DNA concentration in short processing time. Fourthly, plasmid standards were evaluated for use in the qPCR assay. This was done as it was important to consider the efficacy of the oligonucleotides; including the ability of the oligonucleotides to anneal to the appropriate segment of DNA without extensive formation of oligonucleotides dimers, non-specific annealing, or formation of secondary structure. In addition, it was done to ensure that highly accurate standard curves were generated. The standard curves were to be utilised in the downstream qPCR assay to determine the quantity of test samples by interpolation, reading from the values within the standard curve. Lastly, darkfield microscopy and qPCR methods of enumeration were compared to verify their accuracy and determine the most consistent and comparable method. This was achieved by quantifying the purified, crude-purified, and viral formulated CrleGV-SA suspensions using these methods. Subsequently, a statistical analysis was conducted to compare the results produced by the two enumeration methods. The obtained results showed that the granulin, lef- 8 and lef-9 qPCR values did not significantly differ from the darkfield microscopy results. The findings of this study revealed that the two assays, lef-8 qPCR and lef-9 qPCR, were more robust, sensitive, and efficient for the quantification of CrleGV-SA. Thus, this study has successfully developed a qPCR assay that is comparable with the traditional darkfield microscopy counting technique. This is the first study to use the qPCR technique to enumerate CrleGV-SA using plasmid standards. The developed qPCR assay is reliable, rapid, and cost- effective and has a great potential to be used as an alternative method to darkfield microscopy in the laboratory and commercial settings. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
Drifting towards death: a South African patient safety incident through an HFE Systems lens
- Authors: Agar, Sarah Leigh
- Date: 2022-10-14
- Subjects: Patients Safety measures , Medical errors Prevention , Human engineering , Medical care South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362716 , vital:65356
- Description: Patient Safety Incidents (PSI) are a frequent occurrence within the South African public healthcare system wherein a patient is unnecessarily maimed, harmed, killed, or put through significant trauma, emotional or physical. These incidents have a significant impact on the performance of the system and the well-being of individuals involved. Often PSI are the result of multiple system failings that provide the necessary preconditions for the PSI to occur. Thus, to provide appropriate patient safety recommendations to address and aid in the prevention of future PSI it is necessary to apply a systems approach to PSI analysis. A systems approach supports a ‘bigger picture’ view of an incident which includes looking beyond the immediate causes of a PSI and taking the different levels of the healthcare system into consideration during incident analysis. Human Factors and Ergonomics (HFE) is at its core a systems discipline and has been successfully applied to multiple fields including healthcare. HFE offers multiple incident analysis tools grounded in systems theory. The Life Esidimeni incident, a PSI that resulted in the death of 144 MHCU, is the biggest PSI in recent South African history and is therefore an important potential case study for the application of HFE systems tools within the South African healthcare context (an area that is lacking in existing literature). The objectives of this research were to (i) Systematically uncover the causal factors that led to the outcome of the of the Life Esidimeni incident; (ii) Identify critical faults, and gaps within the healthcare system that led to the Life Esidimeni PSI; and (iii) Provide proactive recommendations for future prevention of PSI. To fulfil these objectives a descriptive case study research method design was adopted using a qualitative systems-based tool, AcciMap. The application of AcciMap to Life Esidimeni enabled both the sharp end and blunt end causal factors that contributed to the outcome of the incident to be identified. Importantly this provided insight into the critical faults and gaps of the South African public healthcare system. The results of the AcciMap indicated that there were four main broad systemic faults in the system. These broad areas were categorized as key themes, which include: (i) competency, (ii) safeguards, (iii) time pressures, and (iv) vertical integration. From these key themes recommendations aimed at addressing the critical faults and gaps in the system and preventing future PSI were made. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Agar, Sarah Leigh
- Date: 2022-10-14
- Subjects: Patients Safety measures , Medical errors Prevention , Human engineering , Medical care South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362716 , vital:65356
- Description: Patient Safety Incidents (PSI) are a frequent occurrence within the South African public healthcare system wherein a patient is unnecessarily maimed, harmed, killed, or put through significant trauma, emotional or physical. These incidents have a significant impact on the performance of the system and the well-being of individuals involved. Often PSI are the result of multiple system failings that provide the necessary preconditions for the PSI to occur. Thus, to provide appropriate patient safety recommendations to address and aid in the prevention of future PSI it is necessary to apply a systems approach to PSI analysis. A systems approach supports a ‘bigger picture’ view of an incident which includes looking beyond the immediate causes of a PSI and taking the different levels of the healthcare system into consideration during incident analysis. Human Factors and Ergonomics (HFE) is at its core a systems discipline and has been successfully applied to multiple fields including healthcare. HFE offers multiple incident analysis tools grounded in systems theory. The Life Esidimeni incident, a PSI that resulted in the death of 144 MHCU, is the biggest PSI in recent South African history and is therefore an important potential case study for the application of HFE systems tools within the South African healthcare context (an area that is lacking in existing literature). The objectives of this research were to (i) Systematically uncover the causal factors that led to the outcome of the of the Life Esidimeni incident; (ii) Identify critical faults, and gaps within the healthcare system that led to the Life Esidimeni PSI; and (iii) Provide proactive recommendations for future prevention of PSI. To fulfil these objectives a descriptive case study research method design was adopted using a qualitative systems-based tool, AcciMap. The application of AcciMap to Life Esidimeni enabled both the sharp end and blunt end causal factors that contributed to the outcome of the incident to be identified. Importantly this provided insight into the critical faults and gaps of the South African public healthcare system. The results of the AcciMap indicated that there were four main broad systemic faults in the system. These broad areas were categorized as key themes, which include: (i) competency, (ii) safeguards, (iii) time pressures, and (iv) vertical integration. From these key themes recommendations aimed at addressing the critical faults and gaps in the system and preventing future PSI were made. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
- Date Issued: 2022-10-14
Electrocatalytic activity of symmetric and asymmetric Co(II) and Mn(III) porphyrins in the presence of graphene quantum dots towards the oxidation of hydrazine
- Authors: Jokazi, Mbulelo
- Date: 2022-10-14
- Subjects: Electrocatalysis , Hydrazine , Quantum dots , Graphene , Porphyrins
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362894 , vital:65372
- Description: The influence of metal porphyrins in electro-oxidation of hydrazine is explored. A series of symmetric and asymmetric porphyrins alone and in the presence of graphene quantum dots (GQDs) are employed in this work. Tetra 4-aminophenyl porphyrin, manganese tetra 4-aminophenyl porphyrin, manganese tetra 4-aminophenyl porphyrin--GQDs, and manganese tetra 4-aminophenyl porphyrin@GQDs are the symmetric porphyrins. The asymmetric porphyrin and composites are 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, manganese 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, cobalt 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, manganese 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins--GQDs, and cobalt 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins--GQDs. These complexes were synthesized and characterized accordingly and applied for electrocatalysis. The electrocatalytic experiments were carried out using glassy carbon electrode and the modification was through drop-dry method. The porphyrin and GQDs synthesized were characterized using UV-Vis spectroscopy, Mass spectrometry, X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and energy dispersive x-ray spectroscopy. The modified electrodes were characterized using cyclic voltammetry and electrochemical Impedance spectroscopy. The introduction of metal ion in the center of the porphyrin improved electrocatalysis. The presence of push-pull substituents in the porphyrin lowered the oxidation potential and improved the catalysis. The presence of GQDs improved catalysis in both symmetric and asymmetric porphyrin compared to individual components. Cobalt porphyrins showed better activity than manganese porphyrin. , Thesis (MSc) -- Faculty of Science, Chemistry, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Jokazi, Mbulelo
- Date: 2022-10-14
- Subjects: Electrocatalysis , Hydrazine , Quantum dots , Graphene , Porphyrins
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362894 , vital:65372
- Description: The influence of metal porphyrins in electro-oxidation of hydrazine is explored. A series of symmetric and asymmetric porphyrins alone and in the presence of graphene quantum dots (GQDs) are employed in this work. Tetra 4-aminophenyl porphyrin, manganese tetra 4-aminophenyl porphyrin, manganese tetra 4-aminophenyl porphyrin--GQDs, and manganese tetra 4-aminophenyl porphyrin@GQDs are the symmetric porphyrins. The asymmetric porphyrin and composites are 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, manganese 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, cobalt 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins, manganese 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins--GQDs, and cobalt 5, 10, 15-tris(aminophenyl)-20-(4-carboxyphenyl) porphyrins--GQDs. These complexes were synthesized and characterized accordingly and applied for electrocatalysis. The electrocatalytic experiments were carried out using glassy carbon electrode and the modification was through drop-dry method. The porphyrin and GQDs synthesized were characterized using UV-Vis spectroscopy, Mass spectrometry, X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and energy dispersive x-ray spectroscopy. The modified electrodes were characterized using cyclic voltammetry and electrochemical Impedance spectroscopy. The introduction of metal ion in the center of the porphyrin improved electrocatalysis. The presence of push-pull substituents in the porphyrin lowered the oxidation potential and improved the catalysis. The presence of GQDs improved catalysis in both symmetric and asymmetric porphyrin compared to individual components. Cobalt porphyrins showed better activity than manganese porphyrin. , Thesis (MSc) -- Faculty of Science, Chemistry, 2022
- Full Text:
- Date Issued: 2022-10-14
Elele nje usathetha: Ukukhapha as an expression of amaXhosa language world-sense
- Authors: Dana, Zikho
- Date: 2022-10-14
- Subjects: Xhosa language Discourse analysis , Guides (Spiritualism) , Grief Religious aspects , Historical linguistics , Xhosa language Religious aspects , Xhosa (African people) Death , Death Religious aspects
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406309 , vital:70257
- Description: This study examines ukukhapha as an expression of amaXhosa language world-sense. Firstly, it examines how ukukhapha as a rite of passage to accompany the spirit of the deceased from the physical realm into the spiritual realm maintains the nexus between the living and the dead. Secondly, it explores ukukhapha as a ritual of collective mourning. Both dimensions of the study are pursued using language as a point of departure. This study draws on four key texts written across different times in history, namely: Intlalo kaXhosa (The Social Life of amaXhosa) by Tiyo Burnside (TB) Soga (1974); the three volume Greater Dictionary of isiXhosa (1989, 2003 and 2006); Nokuzola Mndende’s (2002) PhD thesis entitled: Signifying practices: AmaXhosa ritual speech; and Khaya Dlanga’s (2015) memoire: To Quote Myself. This thesis examines ukukhapha in the context of death to establish how amaXhosa speak about death and how this shapes their understanding and its practice. Discourse analysis is employed to clarify some practices and examples of ukukhapha. It’s also used to trace the evolution of ukukhapha over time in isiXhosa. It is utilized as a reservoir of knowledge epitomizing the world-sense of amaXhosa rather than solely as a means of communication. This study reveals that while ukukhapha as a ritual slaughtering of an ox to accompany the deceased is found in literature, its examination as a practice of communal and collective grief is scanty. The four key texts studied in this thesis reveal that ukukhapha goes beyond a mere ritual slaughtering of a sacrificial beast to accompany the spirit of the deceased to the ancestral realm. It is a practice that has allowed amaXhosa to collectively grief. Furthermore, it has provided the bereaved various forms of support which are pivotal in maintaining and strengthening kinship and communal ties among the living. This study further reveals that for the Africans in general, and for amaXhosa in particular, death is not the end of life. It is a continuation of life where the deceased morphs and graduates to an ancestor who joins a larger community of ancestors who act as intermediaries between the living and uMdali, the Creator. The findings demonstrate that through ukukhapha, there is no bifurcation between gender and age, rather they complement each other as the successful execution of the mourning period and farewell of a loved one is dependent on the roles played by both women and men respectively. , Olu phando luceba ukuphonononga ukukhapha njengendlela yokuphila equlathwe kulwimi lwamaXhosa. Okokuqala, luzakuphanda indlela ukukhapha njengesiko lokukhapha umphefumlo womfi ukusukela kwelenyama ukuya kwelemimoya kugcina ngayo ubudlelwane phakathi kwabaphilayo nabaleli ukuthula. Okwesibini, luzakuphanda ukukhapha njengesiko lokuzila ndawoni nye. Zombini ezi nkalo zizakuvelelwa ngokugxila kulwimi. Olu phando lucaphula kwimibhalo emine engundoqo kumaxesha ohlukileyo kwimbali, iquka: Intlalo kaXhosa owabhalwa nguTiyo Burnside Soga (ngowe1974); isichazi-magama esinemiqulu emithathu iThe Greater Dictionary of isiXhosa (ngowama-1989, owama-2003 nowama-2006); ithisisi yePhD kaNokuzola Mndende (ngowama-2002) ebizwa Signifying practices: amaXhosa ritual speech; nencwadi engunomalisa wobom, ebhalwe nguKhaya Dlanga (ngowama-2015) ebizwa To Quote Myself. Le thisisi isebenzisa ukukhapha kwilixa lokufa ukuphanda indlela amaXhosa athetha ngkufa, nokuba le ndlela yokuthetha iyichaphazela njani indlela abakuqonda ngayo ukufa nendlela abakhaphana ngayo. IDiscourse Analysis isetyenziswa njengesixhobo sokucacisa ukukhapha. Iphinde isetyenziswe ukunika imizekelo yeli siko nendlela yokuphila kwakunye nendlela ukukhapha kutshintshe ngayo ekuhambeni kwexesha kulwimi lwesiXhosa njengokuba sizakusetyenziswa njengovimba wolwazi, endaweni yokusetyenziswa njengesixhobo sonxibelelwano kuphela. Olu phando lubonisa ukuba njengokuba ukukhapha njengesiko lokuphalaza igazi lenkomo ukukhapha umfi kubhaliwe ngako kuncwadi, indlela abaphilayo enyameni abakhaphana ngayo iyasilela kuncwadi. Le mibhalo mine echongiweyo nephononongiweyo kolu phando ivelisa ukuba ukukhapha akuphelelanga ekuphalazeni igazi lenkomo kuphela njengesiko lokukhapha umfi ukuze awelele kweleminyanya, yindlela eyavumela amaXhosa ukuba azile ngemanyano apho uluntu luxhasa abafelweyo ngeendlela ezahlukeneyo ezithi zomeleze imanyano phakathi kwabazalanayo noluntu lwabaphilayo. Olu phando lutyhila into yokuba kumaAfrika jikelele, ingakumbi amaXhosa, ukufa ayikuko ukuphela kobom, nto nje luhambo olugqithisa umfi kubom obulandelayo apho ongeza kwinani kwelezinyanya apho izinyanya zilikhonco phakathi koMdali nabaphilayo. Iziphumo ngokukhapha zibonisa ukuba akukho ukuchasana phakathi kwesini neminyaka, nto nje, le miba mibini iyathungelana ekuncediseni nasekuqinisekiseni ukuba abafazi namadoda benza iindima zabo ngokufanelekileyo ekuqinisekiseni ukuba ixesha lezila nokubeka umfi kwikhaya lakhe lokugqibela ziyimpumelelo. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Dana, Zikho
- Date: 2022-10-14
- Subjects: Xhosa language Discourse analysis , Guides (Spiritualism) , Grief Religious aspects , Historical linguistics , Xhosa language Religious aspects , Xhosa (African people) Death , Death Religious aspects
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406309 , vital:70257
- Description: This study examines ukukhapha as an expression of amaXhosa language world-sense. Firstly, it examines how ukukhapha as a rite of passage to accompany the spirit of the deceased from the physical realm into the spiritual realm maintains the nexus between the living and the dead. Secondly, it explores ukukhapha as a ritual of collective mourning. Both dimensions of the study are pursued using language as a point of departure. This study draws on four key texts written across different times in history, namely: Intlalo kaXhosa (The Social Life of amaXhosa) by Tiyo Burnside (TB) Soga (1974); the three volume Greater Dictionary of isiXhosa (1989, 2003 and 2006); Nokuzola Mndende’s (2002) PhD thesis entitled: Signifying practices: AmaXhosa ritual speech; and Khaya Dlanga’s (2015) memoire: To Quote Myself. This thesis examines ukukhapha in the context of death to establish how amaXhosa speak about death and how this shapes their understanding and its practice. Discourse analysis is employed to clarify some practices and examples of ukukhapha. It’s also used to trace the evolution of ukukhapha over time in isiXhosa. It is utilized as a reservoir of knowledge epitomizing the world-sense of amaXhosa rather than solely as a means of communication. This study reveals that while ukukhapha as a ritual slaughtering of an ox to accompany the deceased is found in literature, its examination as a practice of communal and collective grief is scanty. The four key texts studied in this thesis reveal that ukukhapha goes beyond a mere ritual slaughtering of a sacrificial beast to accompany the spirit of the deceased to the ancestral realm. It is a practice that has allowed amaXhosa to collectively grief. Furthermore, it has provided the bereaved various forms of support which are pivotal in maintaining and strengthening kinship and communal ties among the living. This study further reveals that for the Africans in general, and for amaXhosa in particular, death is not the end of life. It is a continuation of life where the deceased morphs and graduates to an ancestor who joins a larger community of ancestors who act as intermediaries between the living and uMdali, the Creator. The findings demonstrate that through ukukhapha, there is no bifurcation between gender and age, rather they complement each other as the successful execution of the mourning period and farewell of a loved one is dependent on the roles played by both women and men respectively. , Olu phando luceba ukuphonononga ukukhapha njengendlela yokuphila equlathwe kulwimi lwamaXhosa. Okokuqala, luzakuphanda indlela ukukhapha njengesiko lokukhapha umphefumlo womfi ukusukela kwelenyama ukuya kwelemimoya kugcina ngayo ubudlelwane phakathi kwabaphilayo nabaleli ukuthula. Okwesibini, luzakuphanda ukukhapha njengesiko lokuzila ndawoni nye. Zombini ezi nkalo zizakuvelelwa ngokugxila kulwimi. Olu phando lucaphula kwimibhalo emine engundoqo kumaxesha ohlukileyo kwimbali, iquka: Intlalo kaXhosa owabhalwa nguTiyo Burnside Soga (ngowe1974); isichazi-magama esinemiqulu emithathu iThe Greater Dictionary of isiXhosa (ngowama-1989, owama-2003 nowama-2006); ithisisi yePhD kaNokuzola Mndende (ngowama-2002) ebizwa Signifying practices: amaXhosa ritual speech; nencwadi engunomalisa wobom, ebhalwe nguKhaya Dlanga (ngowama-2015) ebizwa To Quote Myself. Le thisisi isebenzisa ukukhapha kwilixa lokufa ukuphanda indlela amaXhosa athetha ngkufa, nokuba le ndlela yokuthetha iyichaphazela njani indlela abakuqonda ngayo ukufa nendlela abakhaphana ngayo. IDiscourse Analysis isetyenziswa njengesixhobo sokucacisa ukukhapha. Iphinde isetyenziswe ukunika imizekelo yeli siko nendlela yokuphila kwakunye nendlela ukukhapha kutshintshe ngayo ekuhambeni kwexesha kulwimi lwesiXhosa njengokuba sizakusetyenziswa njengovimba wolwazi, endaweni yokusetyenziswa njengesixhobo sonxibelelwano kuphela. Olu phando lubonisa ukuba njengokuba ukukhapha njengesiko lokuphalaza igazi lenkomo ukukhapha umfi kubhaliwe ngako kuncwadi, indlela abaphilayo enyameni abakhaphana ngayo iyasilela kuncwadi. Le mibhalo mine echongiweyo nephononongiweyo kolu phando ivelisa ukuba ukukhapha akuphelelanga ekuphalazeni igazi lenkomo kuphela njengesiko lokukhapha umfi ukuze awelele kweleminyanya, yindlela eyavumela amaXhosa ukuba azile ngemanyano apho uluntu luxhasa abafelweyo ngeendlela ezahlukeneyo ezithi zomeleze imanyano phakathi kwabazalanayo noluntu lwabaphilayo. Olu phando lutyhila into yokuba kumaAfrika jikelele, ingakumbi amaXhosa, ukufa ayikuko ukuphela kobom, nto nje luhambo olugqithisa umfi kubom obulandelayo apho ongeza kwinani kwelezinyanya apho izinyanya zilikhonco phakathi koMdali nabaphilayo. Iziphumo ngokukhapha zibonisa ukuba akukho ukuchasana phakathi kwesini neminyaka, nto nje, le miba mibini iyathungelana ekuncediseni nasekuqinisekiseni ukuba abafazi namadoda benza iindima zabo ngokufanelekileyo ekuqinisekiseni ukuba ixesha lezila nokubeka umfi kwikhaya lakhe lokugqibela ziyimpumelelo. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Date Issued: 2022-10-14
Evaluating the role of Stress Induced Phosphoprotein 1 isoforms in Kaposi's Sarcoma-Associated Herpesvirus biology
- Authors: Ruck, Duncan Kyle
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365280 , vital:65723
- Description: Thesis embargoed. Possible release date set for early 2025. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Ruck, Duncan Kyle
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365280 , vital:65723
- Description: Thesis embargoed. Possible release date set for early 2025. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
Evaluating the trophic ecology and feeding habits of three divergent lineages of Sandelia bainsii (Teleostei: Anabantidae), from the Eastern Cape Rivers using stable isotope analysis
- Authors: Nkomo, Thulisile
- Date: 2022-10-14
- Subjects: Anabantidae , Trophic ecology , Food chains (Ecology) , Food web , Freshwater fishes Food , Stable isotopes
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364984 , vital:65667
- Description: Despite supporting a disproportionately large fraction of the global biodiversity, freshwater ecosystems are ranked as the most highly threatened habitats on the planet, ahead of both terrestrial and marine ecosystems. Many regions are still characterised by limited knowledge regarding the taxonomy and ecology of freshwater fish taxa. The need for ecological information is increasingly becoming important due to the discovery of new species and unique lineages, which require conservation management. The aim of this study was thus to evaluate the trophic ecology and feeding habits of the three recently described and divergent Sandelia bainsii lineages, namely Sandelia sp. ‘bainsii Kowie’ from the Great Fish River, Sandelia sp. ‘bainsii Keiskamma’ found in the Keiskamma River, and Sandelia sp. ‘bainsii Buffalo’ confined to the Buffalo River system in the Amathole-Winterberg freshwater ecoregion in the Eastern Cape Province, South Africa. Based on the allopatric distribution and the generalist feeding habits of these lineages, this thesis postulated that these three lineages were likely to show similar trophic ecology patterns, different dietary composition and as a result would have variable trophic positioning in the different river systems. Therefore, the primary objectives were to use stable isotope analysis to (1) evaluate the food web patterns of the river systems where the three S. bainsii lineages occurred, and (2) determine dietary source contributions for the three lineages using isotope mixing models. The results revealed general variability and significant differences in the δ13C and δ¹⁵N values for the different basal resources, macroinvertebrates and fish community across the different headwater streams. Within and across the different rivers, the S. bainsii lineages exhibited variable isotopic niche sizes, which appeared to coincide with the variation in the isotopic composition of the individual communities. Furthermore, these lineages did not exhibit any discernible patterns in their interspecific interactions in different habitats. This suggests that these lineages’ isotopic niche patterns were largely influenced by spatial differences in both trophic resources and probable interactions with contraspecifics. Assessment of trophic positions of S. bainsii lineages showed that the three lineages had higher trophic positions than other co-occurring species at most sites, except in the Buffalo River. This suggest that the different lineages were generally top predators in the different river systems. Although S. ‘bainsii Buffalo’ had a lower trophic position compared to other co-occurring species, its trophic position was generally characterised by high uncertainty, indicating that this lineage was likely influenced by the occurrence of diet sources that had highly variable stable isotope values. Findings from stable isotope mixing models revealed that the diet sources varied from the dominance of either single diet source in the Fairburn and Tyume 1 River to the importance of multiple prey sources from the Lushington and Kat River system. This suggests that despite being a top predator at most sites, the diet sources for the different lineages were highly variable. The patterns observed in this study did not appear to be attributed to species divergence possibly caused by allopatric speciation, but rather differences in food web characteristics of the river systems, as well as the lineages interspecific relationships and their generalized feeding strategies. Understanding the trophic dynamics of these lineages will assist in implementing effective conservation strategies and policies dealing with narrowly distributed species that are threatened by habitat fragmentation and invasion of piscivorous fish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Nkomo, Thulisile
- Date: 2022-10-14
- Subjects: Anabantidae , Trophic ecology , Food chains (Ecology) , Food web , Freshwater fishes Food , Stable isotopes
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364984 , vital:65667
- Description: Despite supporting a disproportionately large fraction of the global biodiversity, freshwater ecosystems are ranked as the most highly threatened habitats on the planet, ahead of both terrestrial and marine ecosystems. Many regions are still characterised by limited knowledge regarding the taxonomy and ecology of freshwater fish taxa. The need for ecological information is increasingly becoming important due to the discovery of new species and unique lineages, which require conservation management. The aim of this study was thus to evaluate the trophic ecology and feeding habits of the three recently described and divergent Sandelia bainsii lineages, namely Sandelia sp. ‘bainsii Kowie’ from the Great Fish River, Sandelia sp. ‘bainsii Keiskamma’ found in the Keiskamma River, and Sandelia sp. ‘bainsii Buffalo’ confined to the Buffalo River system in the Amathole-Winterberg freshwater ecoregion in the Eastern Cape Province, South Africa. Based on the allopatric distribution and the generalist feeding habits of these lineages, this thesis postulated that these three lineages were likely to show similar trophic ecology patterns, different dietary composition and as a result would have variable trophic positioning in the different river systems. Therefore, the primary objectives were to use stable isotope analysis to (1) evaluate the food web patterns of the river systems where the three S. bainsii lineages occurred, and (2) determine dietary source contributions for the three lineages using isotope mixing models. The results revealed general variability and significant differences in the δ13C and δ¹⁵N values for the different basal resources, macroinvertebrates and fish community across the different headwater streams. Within and across the different rivers, the S. bainsii lineages exhibited variable isotopic niche sizes, which appeared to coincide with the variation in the isotopic composition of the individual communities. Furthermore, these lineages did not exhibit any discernible patterns in their interspecific interactions in different habitats. This suggests that these lineages’ isotopic niche patterns were largely influenced by spatial differences in both trophic resources and probable interactions with contraspecifics. Assessment of trophic positions of S. bainsii lineages showed that the three lineages had higher trophic positions than other co-occurring species at most sites, except in the Buffalo River. This suggest that the different lineages were generally top predators in the different river systems. Although S. ‘bainsii Buffalo’ had a lower trophic position compared to other co-occurring species, its trophic position was generally characterised by high uncertainty, indicating that this lineage was likely influenced by the occurrence of diet sources that had highly variable stable isotope values. Findings from stable isotope mixing models revealed that the diet sources varied from the dominance of either single diet source in the Fairburn and Tyume 1 River to the importance of multiple prey sources from the Lushington and Kat River system. This suggests that despite being a top predator at most sites, the diet sources for the different lineages were highly variable. The patterns observed in this study did not appear to be attributed to species divergence possibly caused by allopatric speciation, but rather differences in food web characteristics of the river systems, as well as the lineages interspecific relationships and their generalized feeding strategies. Understanding the trophic dynamics of these lineages will assist in implementing effective conservation strategies and policies dealing with narrowly distributed species that are threatened by habitat fragmentation and invasion of piscivorous fish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2022
- Full Text:
- Date Issued: 2022-10-14