Retail pharmacists’ perceptions of factors influencing the viability of practice and business in Johannesburg, Gauteng province
- Gazi, Nondumiso Sybil, Knoesen, BC
- Authors: Gazi, Nondumiso Sybil , Knoesen, BC
- Date: 2019
- Subjects: Pharmacists -- South Africa -- Johannesburg , Pharmaceutical services -- South Africa -- Johannesburg -- Management Pharmacy management -- South Africa -- Johannesburg
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/39774 , vital:35454
- Description: The practice of retail pharmacy has become burdened with service limitations directly or indirectly resulting from influences across the landscape. Influences ranging from legislative bodies to financing bodies have created pressure on retail pharmacies resulting in diminishing numbers of these health establishments. Legislative amendments relating to the ownership of pharmacies, pricing and pharmacy personnel training have had a profound effect on the pharmacy landscape as it presents to date. The dual role, (medicinal procurer and pharmaceutical caregiver), required of retail pharmacists, poses ethical and practical implications. These implications may directly affect the sustainability of retail pharmacy practice. The aim of this study was to establish the ways in which the pharmacy model is changing and how that change has been perceived by retail pharmacists in Johannesburg, Gauteng. The research design was qualitative, exploratory, descriptive and contextual in nature. The method utilised for gathering data was individual interviews with pharmacists practising as independent retail pharmacists. At least six (6) independent pharmacists who met the inclusion criteria were approached to participate in the research. Interviews continued until data saturation had been achieved. Data analysis of the transcribed interviews was performed to identify themes and sub-themes.
- Full Text:
- Date Issued: 2019
- Authors: Gazi, Nondumiso Sybil , Knoesen, BC
- Date: 2019
- Subjects: Pharmacists -- South Africa -- Johannesburg , Pharmaceutical services -- South Africa -- Johannesburg -- Management Pharmacy management -- South Africa -- Johannesburg
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/39774 , vital:35454
- Description: The practice of retail pharmacy has become burdened with service limitations directly or indirectly resulting from influences across the landscape. Influences ranging from legislative bodies to financing bodies have created pressure on retail pharmacies resulting in diminishing numbers of these health establishments. Legislative amendments relating to the ownership of pharmacies, pricing and pharmacy personnel training have had a profound effect on the pharmacy landscape as it presents to date. The dual role, (medicinal procurer and pharmaceutical caregiver), required of retail pharmacists, poses ethical and practical implications. These implications may directly affect the sustainability of retail pharmacy practice. The aim of this study was to establish the ways in which the pharmacy model is changing and how that change has been perceived by retail pharmacists in Johannesburg, Gauteng. The research design was qualitative, exploratory, descriptive and contextual in nature. The method utilised for gathering data was individual interviews with pharmacists practising as independent retail pharmacists. At least six (6) independent pharmacists who met the inclusion criteria were approached to participate in the research. Interviews continued until data saturation had been achieved. Data analysis of the transcribed interviews was performed to identify themes and sub-themes.
- Full Text:
- Date Issued: 2019
The influence of power distance relationships on the success of lean manufacturing implementations
- Authors: De Beer, Lourens
- Date: 2016
- Subjects: Lean manufacturing , Corporate culture
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/6919 , vital:21166
- Description: The research project measured the influence of lean culture elements as well as power distance elements on the success of lean manufacturing implementations. The literature review revealed that lean transformations are not always successful and sustainable since organisation see these as quick win opportunities to improve short term profits. Lean, however, is a long term philosophy that entails not just quick changes but a fundamental change in the way that business is done. The elements that were measured in the study were organisational awareness, employee engagement, managerial consistency, accountability, mutual respect and autocratic behaviour. The study revealed a strong relationship between these factors and the success of lean implementations. The results indicated that there is a positive relationship between lean culture and the other lean elements. The study also indicated that autocratic behaviour has a positive relationship to lean implementation. The study showed that tools that were developed in the past are valid across various industries and that power distance does play a role in lean implementations.
- Full Text:
- Date Issued: 2016
- Authors: De Beer, Lourens
- Date: 2016
- Subjects: Lean manufacturing , Corporate culture
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/6919 , vital:21166
- Description: The research project measured the influence of lean culture elements as well as power distance elements on the success of lean manufacturing implementations. The literature review revealed that lean transformations are not always successful and sustainable since organisation see these as quick win opportunities to improve short term profits. Lean, however, is a long term philosophy that entails not just quick changes but a fundamental change in the way that business is done. The elements that were measured in the study were organisational awareness, employee engagement, managerial consistency, accountability, mutual respect and autocratic behaviour. The study revealed a strong relationship between these factors and the success of lean implementations. The results indicated that there is a positive relationship between lean culture and the other lean elements. The study also indicated that autocratic behaviour has a positive relationship to lean implementation. The study showed that tools that were developed in the past are valid across various industries and that power distance does play a role in lean implementations.
- Full Text:
- Date Issued: 2016
The anticancer activity of Cyathula prostrata on two malignant cell lines
- Authors: Schnablegger, Gerald
- Date: 2010
- Subjects: Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10309 , http://hdl.handle.net/10948/1563 , Medicinal plants
- Description: Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract has been linked to cell cycle arrest in the G1 phase at 24 and 48 h. In both cell lines, more than 80 percent of the C. prostrata treated cells were found in the G1 phase after 48 hours of treatment. The annexin V-FITC/PI assay revealed an increase in the percentage apoptotic cells from 4.9 percent to 53.1 percent at 24 h and 8.3 percent to 50.3 percent at 48 h. Since apoptosis induction can occur via a number of different pathways, distinct features were used as markers to investigate the mode of action of this C. prostrata extract. Markers such as activated caspase-8, p21 and cyt-c, were investigated with the aid of fluorescently labelled (FITC) antibodies with analysis using flow cytometry. No change in p21 levels was observed in response to treatment with the extract for up to 48 h. Cell cycle arrest in G1 was therefore not induced by this cyclin-CDK inhibitor. Increase in caspase-8 activation was observed in response to treatment with the extract with no cyt-c release from the mitochondria. The lack of cyt-c release was due to no change in mitochondrial membrane potential, which was investigated with the aid of fluorescent mitochondrial dyes and flow cytometric techniques. Caspase-8 activation is unique to the extrinsic apoptotic pathway. The results from this study therefore show that C. prostrata extract induces apoptosis via the extrinsic pathway and that this activation in independent of the mitochondria. The levels of hTERT, the catalytic subunit of telomerase, were investigated as an additional molecular target for C. prostrata. This was also investigated using FITC labelled antibodies and flow cytometry. A decrease in hTERT levels was observed following C. prostrata treatment. The findings from this study suggest that the extract acts through multiple targets, by inducing: cell cycle arrest in the G1 phase through an unknown mechanism; apoptosis through an extrinsic death receptor pathway and replicative senescence through inhibition of telomerase.
- Full Text:
- Date Issued: 2010
- Authors: Schnablegger, Gerald
- Date: 2010
- Subjects: Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10309 , http://hdl.handle.net/10948/1563 , Medicinal plants
- Description: Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract has been linked to cell cycle arrest in the G1 phase at 24 and 48 h. In both cell lines, more than 80 percent of the C. prostrata treated cells were found in the G1 phase after 48 hours of treatment. The annexin V-FITC/PI assay revealed an increase in the percentage apoptotic cells from 4.9 percent to 53.1 percent at 24 h and 8.3 percent to 50.3 percent at 48 h. Since apoptosis induction can occur via a number of different pathways, distinct features were used as markers to investigate the mode of action of this C. prostrata extract. Markers such as activated caspase-8, p21 and cyt-c, were investigated with the aid of fluorescently labelled (FITC) antibodies with analysis using flow cytometry. No change in p21 levels was observed in response to treatment with the extract for up to 48 h. Cell cycle arrest in G1 was therefore not induced by this cyclin-CDK inhibitor. Increase in caspase-8 activation was observed in response to treatment with the extract with no cyt-c release from the mitochondria. The lack of cyt-c release was due to no change in mitochondrial membrane potential, which was investigated with the aid of fluorescent mitochondrial dyes and flow cytometric techniques. Caspase-8 activation is unique to the extrinsic apoptotic pathway. The results from this study therefore show that C. prostrata extract induces apoptosis via the extrinsic pathway and that this activation in independent of the mitochondria. The levels of hTERT, the catalytic subunit of telomerase, were investigated as an additional molecular target for C. prostrata. This was also investigated using FITC labelled antibodies and flow cytometry. A decrease in hTERT levels was observed following C. prostrata treatment. The findings from this study suggest that the extract acts through multiple targets, by inducing: cell cycle arrest in the G1 phase through an unknown mechanism; apoptosis through an extrinsic death receptor pathway and replicative senescence through inhibition of telomerase.
- Full Text:
- Date Issued: 2010
- «
- ‹
- 1
- ›
- »