The effects of vascular targeted bi-functionalized aunps on hepatic lipid metabolism of a diet-induced rats
- Authors: Singo, Dakalo Gladwell
- Date: 2020
- Subjects: Bile acids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47072 , vital:39780
- Description: Introduction: Obesity is a metabolic disease in which the accumulation of excessive fat in the adipose tissue may interfere with the normal maintenance of a healthy state. However, there is only a limited number of drugs approved for the treatment of obesity. For this purpose, target therapies are receiving more attention as a preferred treatment to reduce the burden of chronic diseases which are associated with the current strategies. Nanotechnology is a highly promising field that offers a nanotechnology-based drug delivery system as a novel means of solving issues associated with the toxicity of conventional anti-obesity drugs. Vascular targeted nanotherapy (targeted bi-functionalized nanoparticles) as a strategy that can overcome the current available anti-obesity drugs. Aim: This study sought to understand the effects of nanotherapy in the hepatic lipid metabolism and secretion of bile acids Research focus: The effects of gold nanotherapy on the bile acid biosynthetic enzymes, transporters and lipogenic enzymes of rats that were on a high fat diet. Conclusion: The study shows low expression in the majority of genes responsible for bile acid synthesis and a lipogenic pathway; therefore, the effects of nanotherapy exhibit low toxicity in the hepatic lipid metabolism.
- Full Text:
- Date Issued: 2020
- Authors: Singo, Dakalo Gladwell
- Date: 2020
- Subjects: Bile acids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47072 , vital:39780
- Description: Introduction: Obesity is a metabolic disease in which the accumulation of excessive fat in the adipose tissue may interfere with the normal maintenance of a healthy state. However, there is only a limited number of drugs approved for the treatment of obesity. For this purpose, target therapies are receiving more attention as a preferred treatment to reduce the burden of chronic diseases which are associated with the current strategies. Nanotechnology is a highly promising field that offers a nanotechnology-based drug delivery system as a novel means of solving issues associated with the toxicity of conventional anti-obesity drugs. Vascular targeted nanotherapy (targeted bi-functionalized nanoparticles) as a strategy that can overcome the current available anti-obesity drugs. Aim: This study sought to understand the effects of nanotherapy in the hepatic lipid metabolism and secretion of bile acids Research focus: The effects of gold nanotherapy on the bile acid biosynthetic enzymes, transporters and lipogenic enzymes of rats that were on a high fat diet. Conclusion: The study shows low expression in the majority of genes responsible for bile acid synthesis and a lipogenic pathway; therefore, the effects of nanotherapy exhibit low toxicity in the hepatic lipid metabolism.
- Full Text:
- Date Issued: 2020
Perceptions of tourism in the Nelson Mandela Bay Municipality with special reference to the Red location in New Brighton
- Authors: Mfo, Ntomboxolo Siphokazi
- Date: 2019
- Subjects: Heritage tourism -- South Africa -- Port Elizabeth , New Brighton (Port Elizabeth, South Africa) Blacks -- South Africa -- Port Elizabeth -- Social life and customs Tourism -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10948/41316 , vital:36443
- Description: Township tourism is a relatively growing sector of the tourism market; therefore, many large cities in developed and developing countries, such as Brazil, have adopted tourism-led approaches to township tourism. Township tourism now forms part of the South African local and regional economic development strategies of some of the major cities, such as Johannesburg, Cape Town and Port Elizabeth. In turn, it is perceived as the fastest growing type of tourism in the world and can function as a powerful tool in township development and transformation. Township tourism has the potential to produce economic benefits, improve physical environment, inner-city image and the welfare of community members. The focus of the research was to study the perception of tourism in the Nelson Mandela Bay Municipality with special reference to the community of the Red Location in New Brighton. Additionally, this study explored to understand how tourism stakeholders (government, provincial government and local municipality), tourism development agencies, as well as the community of the Red Location, could use tourism as a tool for township development, local economic growth and to increase visitors to the Nelson Mandela Bay area. The research adopted a qualitative research method. The research builds on an extensive review of a broad range of secondary sources from different academic and institutional backgrounds to give a more objective and nuanced analysis. The qualitative literature that was used include, but is not limited to, academic articles, books, official reports from the various tourism organisations, publications from tourism authorities, news media, South African legislation and publications from government organisations. Based on this study, it was found that tourism has the capacity to rejuvenate the area and contribute to the local economic development, poverty reduction, as well as create the identity of a place, and serve as a part of the solution for township problems. The potential of tourism to stimulate township development strongly depends on the presence of tourism resources that can be developed into consumable products or visitor attraction as well as on the financial capacity of public and private partners to strengthen and increase visitor facilities, infrastructure and activities. The study employed a descriptive study to capture detailed and meaningful information from the literature search. The literature search gave the researcher an understanding of the research area, tourism policies and the role of the communities in tourism. This understanding improved the quality of the study. Furthermore, the literature provides township tourism precondition guidelines which may be followed to develop tourism in South Africa, especially in communities, such as the Red Location. Overall, the results of this study demonstrate the need to draft and adopt a township tourism strategy, one that is unique to the culture and community of the Red Location in New Brighton.
- Full Text:
- Date Issued: 2019
- Authors: Mfo, Ntomboxolo Siphokazi
- Date: 2019
- Subjects: Heritage tourism -- South Africa -- Port Elizabeth , New Brighton (Port Elizabeth, South Africa) Blacks -- South Africa -- Port Elizabeth -- Social life and customs Tourism -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10948/41316 , vital:36443
- Description: Township tourism is a relatively growing sector of the tourism market; therefore, many large cities in developed and developing countries, such as Brazil, have adopted tourism-led approaches to township tourism. Township tourism now forms part of the South African local and regional economic development strategies of some of the major cities, such as Johannesburg, Cape Town and Port Elizabeth. In turn, it is perceived as the fastest growing type of tourism in the world and can function as a powerful tool in township development and transformation. Township tourism has the potential to produce economic benefits, improve physical environment, inner-city image and the welfare of community members. The focus of the research was to study the perception of tourism in the Nelson Mandela Bay Municipality with special reference to the community of the Red Location in New Brighton. Additionally, this study explored to understand how tourism stakeholders (government, provincial government and local municipality), tourism development agencies, as well as the community of the Red Location, could use tourism as a tool for township development, local economic growth and to increase visitors to the Nelson Mandela Bay area. The research adopted a qualitative research method. The research builds on an extensive review of a broad range of secondary sources from different academic and institutional backgrounds to give a more objective and nuanced analysis. The qualitative literature that was used include, but is not limited to, academic articles, books, official reports from the various tourism organisations, publications from tourism authorities, news media, South African legislation and publications from government organisations. Based on this study, it was found that tourism has the capacity to rejuvenate the area and contribute to the local economic development, poverty reduction, as well as create the identity of a place, and serve as a part of the solution for township problems. The potential of tourism to stimulate township development strongly depends on the presence of tourism resources that can be developed into consumable products or visitor attraction as well as on the financial capacity of public and private partners to strengthen and increase visitor facilities, infrastructure and activities. The study employed a descriptive study to capture detailed and meaningful information from the literature search. The literature search gave the researcher an understanding of the research area, tourism policies and the role of the communities in tourism. This understanding improved the quality of the study. Furthermore, the literature provides township tourism precondition guidelines which may be followed to develop tourism in South Africa, especially in communities, such as the Red Location. Overall, the results of this study demonstrate the need to draft and adopt a township tourism strategy, one that is unique to the culture and community of the Red Location in New Brighton.
- Full Text:
- Date Issued: 2019
Method validation for the quantification of casticin in vitex agnus castus fruit using an ftir multivariate chemometric model
- Authors: Du Toit Louw, Philippus
- Date: 2018
- Subjects: Drugs -- Analysis -- Methodology -- Evaluation , Alternative medicine -- Research , Liquid chromatography
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10948/30917 , vital:31222
- Description: The Complementary and Alternative Medicines (CAMS) and dietary supplement industry is an R8.84 billion a year industry in South Africa, with the sector expected to grow 12% annually. The industry has largely been unregulated up until 15 November 2013, when the South African government amended the Medicines and Related Substance Control Act (Act 101 of 1965) to include the definition of “Complementary Medicines” and “Health Supplements”. The emphasis of the new regulations is largely on the quality and safety of CAMS products. Manufacturers therefore need to demonstrate that the active ingredients used in manufacturing will be of an appropriate and consistent quality. The research question therefore is: Can a chemometric multivariate model be used to develop a rapid, cost effective method to quantify casticin, the major chemical constituent of Vitex agnus-castus (VAC) that can be used during routine quality control procedures? The primary aim of this study was to prepare a validated method using fourier transform infrared spectroscopy (FTIR) to quantify the casticin content in VAC fruit. The results from the HPLC analysis were as follows; the penduletin eluted at a retention time of 12.419 ± 0.376 minutes. The casticin eluted at a retention time of 12.943 ± 0.018 minutes. The casticin content for the samples ranged from 0.0115 – 0.0147% m/m casticin content with an average of 0.0134%. This is well below the pharmacopoeia requirement of not less than (NLT) 0.08% casticin content as described in the British Pharmacopoeia (BP). The results obtained from the HPLC analysis were used to construct the FTIR calibration model. The calibration model consisted of 18 spectra with 530 selected data points. The model was specific for casticin as spectral regions in the calibration model can be correlated to a known IR spectral response associated with the carbonyl group of casticin. The calibration equation in the xii form of % m/m casticin content had a coefficient of determination (R2) value of 0.9855 and a root mean square error of cross-validation (RMSECV) of 0.000119. The accuracy of the model had recoveries of between 98 - 102% for the actual vs true prediction. The percentage relative standard deviation (%RSD) between nine repeated measurements was 3.46%, this does not meet the International Conference of Harmonisation (ICH) requirement for precision of not more than (NMT) 2% RSD. The range of the calibration model was between 0.01147 and 0.01469 % m/m casticin content as established by the calibration model. The robustness of the method was assessed by challenging the model with samples that fall outside of the concentration of range of the model. This was established by quantifying previously scanned samples of VAC that is not part of the calibration set. The model was able to verify if the tested samples prediction was outside of the validated calibration range. The method was subsequently also challenged with a sample of a different identity to VAC. The model indicated that the sample tested does not fall in the range of the method and was clearly recognised as an outlier. The method was rapid and does not require any expensive solvents or timeconsuming sample preparation. However, the method does not meet the ICH requirements for method validation, the method does show potential and further method development and expansion of the calibration model can ensure that the method be validated.
- Full Text:
- Date Issued: 2018
- Authors: Du Toit Louw, Philippus
- Date: 2018
- Subjects: Drugs -- Analysis -- Methodology -- Evaluation , Alternative medicine -- Research , Liquid chromatography
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10948/30917 , vital:31222
- Description: The Complementary and Alternative Medicines (CAMS) and dietary supplement industry is an R8.84 billion a year industry in South Africa, with the sector expected to grow 12% annually. The industry has largely been unregulated up until 15 November 2013, when the South African government amended the Medicines and Related Substance Control Act (Act 101 of 1965) to include the definition of “Complementary Medicines” and “Health Supplements”. The emphasis of the new regulations is largely on the quality and safety of CAMS products. Manufacturers therefore need to demonstrate that the active ingredients used in manufacturing will be of an appropriate and consistent quality. The research question therefore is: Can a chemometric multivariate model be used to develop a rapid, cost effective method to quantify casticin, the major chemical constituent of Vitex agnus-castus (VAC) that can be used during routine quality control procedures? The primary aim of this study was to prepare a validated method using fourier transform infrared spectroscopy (FTIR) to quantify the casticin content in VAC fruit. The results from the HPLC analysis were as follows; the penduletin eluted at a retention time of 12.419 ± 0.376 minutes. The casticin eluted at a retention time of 12.943 ± 0.018 minutes. The casticin content for the samples ranged from 0.0115 – 0.0147% m/m casticin content with an average of 0.0134%. This is well below the pharmacopoeia requirement of not less than (NLT) 0.08% casticin content as described in the British Pharmacopoeia (BP). The results obtained from the HPLC analysis were used to construct the FTIR calibration model. The calibration model consisted of 18 spectra with 530 selected data points. The model was specific for casticin as spectral regions in the calibration model can be correlated to a known IR spectral response associated with the carbonyl group of casticin. The calibration equation in the xii form of % m/m casticin content had a coefficient of determination (R2) value of 0.9855 and a root mean square error of cross-validation (RMSECV) of 0.000119. The accuracy of the model had recoveries of between 98 - 102% for the actual vs true prediction. The percentage relative standard deviation (%RSD) between nine repeated measurements was 3.46%, this does not meet the International Conference of Harmonisation (ICH) requirement for precision of not more than (NMT) 2% RSD. The range of the calibration model was between 0.01147 and 0.01469 % m/m casticin content as established by the calibration model. The robustness of the method was assessed by challenging the model with samples that fall outside of the concentration of range of the model. This was established by quantifying previously scanned samples of VAC that is not part of the calibration set. The model was able to verify if the tested samples prediction was outside of the validated calibration range. The method was subsequently also challenged with a sample of a different identity to VAC. The model indicated that the sample tested does not fall in the range of the method and was clearly recognised as an outlier. The method was rapid and does not require any expensive solvents or timeconsuming sample preparation. However, the method does not meet the ICH requirements for method validation, the method does show potential and further method development and expansion of the calibration model can ensure that the method be validated.
- Full Text:
- Date Issued: 2018
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