The antifungal activity of an aqueous Tulbaghia violacea plant extract against Aspergillus flavus
- Authors: Belewa, Xoliswa Vuyokazi
- Date: 2015
- Subjects: Medicinal plants , Antifungal agents , Fungi -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/5858 , vital:21001
- Description: Phytochemical analysis of both HEA1 and the crude plant extract showed the presence of phenolics, tannins and saponins. Saponins were the predominant secondary metabolites and were mostly abundant in the plant extract and to a lesser extent in the active compound. Steroidal saponins, tannins and phenolics were also detected in the plant extract, but only the phenolics were detected in the active compound. The results of the phytochemical analysis showed that those compounds that were not present in the active compound could be removed from the crude extract during the TLC purification process. Investigation on the mechanism of action of the crude plant extract on the sterol production by A. flavus showed that the plant extract affected ergosterol biosynthesis by causing an accumulation of oxidosqualene in the ergosterol biosynthetic pathway resulting in a decline in ergosterol production. An oscillatory response in lanosterol production was observed in the presence of the plant extract, which may be an adaptation mechanism of A. flavus to unfavourable conditions and compensation for the loss of enzyme activity which may have occurred as a result of the accumulation of oxidosqualene. The antifungal activity of the plant extract on ergosterol production by A. flavus may also be due to saponins which target the cell membrane and ergosterol production in fungi. The effect of the plant extract on the fungal cell wall of A. flavus also showed that the plant extract caused a decline in β-(1, 3) glucan production by inhibiting β-glucan synthase. The plant extract also affected the chitin synthesis pathway of A. flavus, by causing a decline in chitin production, which was due to the inhibition of chitin synthase. Investigation of chitinase production using 4MU substrates showed that the plant extract caused an accumulation of chitobioses, by activating chitobiosidases and endochitinases. A decline in N-acetylglucosaminidase activity in the presence of the plant extract was observed and this prevented the formation of N-acetylglucosamine. The accumulation of chitobiosidase and endochitinase may be as a result of autolysis that may be triggered by A. flavus as a survival mechanism in the presence of the plant extract and as a compensatory mechanism for the loss of β-glucans and chitin. The antifungal effect of the plant extract on various components of the cell wall of A. flavus, makes T. violacea aqueous plant extract an ideal chemotherapeutic agent against both human and plant pathogens of Aspergillus. The broad spectrum of antifungal activity of T. violacea against A. flavus also eliminates any chances of the fungus developing resistance towards it and would make it a candidate for use as a potential antifungal agent. Further identification and possible chemical synthesis is needed to shed light on the safety and efficacy of the active compound for further development as a chemotherapeutic agent.
- Full Text:
- Date Issued: 2015
- Authors: Belewa, Xoliswa Vuyokazi
- Date: 2015
- Subjects: Medicinal plants , Antifungal agents , Fungi -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/5858 , vital:21001
- Description: Phytochemical analysis of both HEA1 and the crude plant extract showed the presence of phenolics, tannins and saponins. Saponins were the predominant secondary metabolites and were mostly abundant in the plant extract and to a lesser extent in the active compound. Steroidal saponins, tannins and phenolics were also detected in the plant extract, but only the phenolics were detected in the active compound. The results of the phytochemical analysis showed that those compounds that were not present in the active compound could be removed from the crude extract during the TLC purification process. Investigation on the mechanism of action of the crude plant extract on the sterol production by A. flavus showed that the plant extract affected ergosterol biosynthesis by causing an accumulation of oxidosqualene in the ergosterol biosynthetic pathway resulting in a decline in ergosterol production. An oscillatory response in lanosterol production was observed in the presence of the plant extract, which may be an adaptation mechanism of A. flavus to unfavourable conditions and compensation for the loss of enzyme activity which may have occurred as a result of the accumulation of oxidosqualene. The antifungal activity of the plant extract on ergosterol production by A. flavus may also be due to saponins which target the cell membrane and ergosterol production in fungi. The effect of the plant extract on the fungal cell wall of A. flavus also showed that the plant extract caused a decline in β-(1, 3) glucan production by inhibiting β-glucan synthase. The plant extract also affected the chitin synthesis pathway of A. flavus, by causing a decline in chitin production, which was due to the inhibition of chitin synthase. Investigation of chitinase production using 4MU substrates showed that the plant extract caused an accumulation of chitobioses, by activating chitobiosidases and endochitinases. A decline in N-acetylglucosaminidase activity in the presence of the plant extract was observed and this prevented the formation of N-acetylglucosamine. The accumulation of chitobiosidase and endochitinase may be as a result of autolysis that may be triggered by A. flavus as a survival mechanism in the presence of the plant extract and as a compensatory mechanism for the loss of β-glucans and chitin. The antifungal effect of the plant extract on various components of the cell wall of A. flavus, makes T. violacea aqueous plant extract an ideal chemotherapeutic agent against both human and plant pathogens of Aspergillus. The broad spectrum of antifungal activity of T. violacea against A. flavus also eliminates any chances of the fungus developing resistance towards it and would make it a candidate for use as a potential antifungal agent. Further identification and possible chemical synthesis is needed to shed light on the safety and efficacy of the active compound for further development as a chemotherapeutic agent.
- Full Text:
- Date Issued: 2015
Soil fertility enhancement through appropriate fertilizer management on winter cover crops in a conservation agriculture system
- Authors: Dube, Ernest
- Date: 2012
- Subjects: Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Language: English
- Type: Thesis , Doctoral , PhD (Crop Science)
- Identifier: vital:11239 , http://hdl.handle.net/10353/d1001044 , Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Description: A study was carried out to determine the effects of oat (Avena sativa) and grazing vetch (Vicia darsycapa) winter cover crops and fertilizer application on SOM, phosphorus (P) pools, nutrient availability, nutrient uptake, maize yield and seedbanks of problematic weeds in an irrigated maize-based conservation agriculture (CA) system. A separate experiment was carried out to investigate the effects of the winter cover crops on nitrogen (N) management, N use efficiency and profitability. After four years of continuous rotation, the winter cover crops significantly (p<0.05) increased particulate SOM and hot water soluble carbon in the 0 – 5 and 5 – 20 cm soil depths. When fertilized, oat was better able to support SOM sequestration in water stable aggregates at 0 – 20 cm whilst grazing vetch was more effective at 20 – 50 cm. Where no fertilizer was invested, there were significant (p<0.01) reductions in biomass input and SOM on oat-maize and weedy fallow-maize rotations whereas vetch-maize rotations did not respond, both at 0 – 5 and 5 – 20 cm. Targeting fertilizer to the winter cover crop required less fertilizer, and yet gave a similar SOM response as targeting the fertilizer to the maize crop. In addition to increasing SOM in the surface soil (0 – 5 cm), the winter cover crops significantly (p<0.05) increased labile pools of P, including microbial P. The cover crops also significantly (p<0.05) increased maize P concentration during early growth, extractable soil P, Cu, Mn, and Zn but had no effect on Ca and K. Grazing vetch increased soil mineral N but reduced extractable soil Mg. Without fertilizer, there were sharp declines in maize grain yield on oat and weedy fallow rotations over the four year period, but less so, on the grazing vetch. Grazing vetch increased maize growth, grain yield response to N fertilizer, nitrogen use efficiency (NUE) and profitability for fertilizer rates below 180 kg N ha-1. Oat effects however on maize yield and NUE were generally similar to weedy fallow. Based on the partial factor productivity of N, the highest efficiencies in utilization of fertilizer N for maize yield improvement under grazing vetch and oat are obtained at 60 kg N ha-1 and would decline thereafter with any increases in fertilizer application rate. Grazing vetch gave N fertilizer replacement values of up to 120 kg N ha-1 as well as the highest marginal rates of return to increasing N fertilizer rate. The cover crops were more effective than the weedy fallow in reducing seedbank density of Digitaria sanguinalis, Eleusine indica, Amaranthus retroflexus and Datura stramonium at 0 – 5 cm soil depth, causing weed seed reductions of 30 - 70%. The winter cover crops however, selectively allowed emergence of the narrow leafed weeds; Cyperus esculentus and Digitaria sanguinalis in the maize crop. The findings of this study suggested that grazing vetch is suited for SOM improvement in low fertilizer input systems and that fertilizer is better invested on winter cover crops as opposed to maize crops. Oat, on the other hand, when fertilized, would be ideal for C sequestration in water stable aggregates of the surface soil. Grazing vetch is ideal for resource poor farmers who cannot afford mineral fertilizers as it gives grain yield improvement and high fertilizer replacement value. Grazing vetch can produce enough maize yield response to pay its way in the maize-based systems and oat may not require additional N than that applied to the weedy fallow. Phosphorus and Zn are some of the major limiting essential plant nutrients on South African soils and the winter cover crops could make a contribution. The cover crops also hasten depletion of some problematic weeds from seedbanks, leading to reduced weed pressure during maize growth.
- Full Text:
- Date Issued: 2012
- Authors: Dube, Ernest
- Date: 2012
- Subjects: Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Language: English
- Type: Thesis , Doctoral , PhD (Crop Science)
- Identifier: vital:11239 , http://hdl.handle.net/10353/d1001044 , Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Description: A study was carried out to determine the effects of oat (Avena sativa) and grazing vetch (Vicia darsycapa) winter cover crops and fertilizer application on SOM, phosphorus (P) pools, nutrient availability, nutrient uptake, maize yield and seedbanks of problematic weeds in an irrigated maize-based conservation agriculture (CA) system. A separate experiment was carried out to investigate the effects of the winter cover crops on nitrogen (N) management, N use efficiency and profitability. After four years of continuous rotation, the winter cover crops significantly (p<0.05) increased particulate SOM and hot water soluble carbon in the 0 – 5 and 5 – 20 cm soil depths. When fertilized, oat was better able to support SOM sequestration in water stable aggregates at 0 – 20 cm whilst grazing vetch was more effective at 20 – 50 cm. Where no fertilizer was invested, there were significant (p<0.01) reductions in biomass input and SOM on oat-maize and weedy fallow-maize rotations whereas vetch-maize rotations did not respond, both at 0 – 5 and 5 – 20 cm. Targeting fertilizer to the winter cover crop required less fertilizer, and yet gave a similar SOM response as targeting the fertilizer to the maize crop. In addition to increasing SOM in the surface soil (0 – 5 cm), the winter cover crops significantly (p<0.05) increased labile pools of P, including microbial P. The cover crops also significantly (p<0.05) increased maize P concentration during early growth, extractable soil P, Cu, Mn, and Zn but had no effect on Ca and K. Grazing vetch increased soil mineral N but reduced extractable soil Mg. Without fertilizer, there were sharp declines in maize grain yield on oat and weedy fallow rotations over the four year period, but less so, on the grazing vetch. Grazing vetch increased maize growth, grain yield response to N fertilizer, nitrogen use efficiency (NUE) and profitability for fertilizer rates below 180 kg N ha-1. Oat effects however on maize yield and NUE were generally similar to weedy fallow. Based on the partial factor productivity of N, the highest efficiencies in utilization of fertilizer N for maize yield improvement under grazing vetch and oat are obtained at 60 kg N ha-1 and would decline thereafter with any increases in fertilizer application rate. Grazing vetch gave N fertilizer replacement values of up to 120 kg N ha-1 as well as the highest marginal rates of return to increasing N fertilizer rate. The cover crops were more effective than the weedy fallow in reducing seedbank density of Digitaria sanguinalis, Eleusine indica, Amaranthus retroflexus and Datura stramonium at 0 – 5 cm soil depth, causing weed seed reductions of 30 - 70%. The winter cover crops however, selectively allowed emergence of the narrow leafed weeds; Cyperus esculentus and Digitaria sanguinalis in the maize crop. The findings of this study suggested that grazing vetch is suited for SOM improvement in low fertilizer input systems and that fertilizer is better invested on winter cover crops as opposed to maize crops. Oat, on the other hand, when fertilized, would be ideal for C sequestration in water stable aggregates of the surface soil. Grazing vetch is ideal for resource poor farmers who cannot afford mineral fertilizers as it gives grain yield improvement and high fertilizer replacement value. Grazing vetch can produce enough maize yield response to pay its way in the maize-based systems and oat may not require additional N than that applied to the weedy fallow. Phosphorus and Zn are some of the major limiting essential plant nutrients on South African soils and the winter cover crops could make a contribution. The cover crops also hasten depletion of some problematic weeds from seedbanks, leading to reduced weed pressure during maize growth.
- Full Text:
- Date Issued: 2012
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