Investigation into the characteristics and possible applications of biomass gasification by-products from a downdraft gasifier system
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
Isolation and characterisation of lignocellulose degrading bacteria from Tyume River in the Eastern Cape Province, South Africa
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
Isolation and molecular characterization of Bacillus cereus from cow’s raw milk
- Authors: Lukanji, Zinathi , Ndip, R N
- Date: 2015
- Subjects: Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11296 , http://hdl.handle.net/10353/d1021284 , Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Description: Bacillus cereus is a group of ubiquitous facultative anaerobic spore forming Gram-positive rods commonly found in soil. It has been detected and implicated in several contaminated food products and raw milk in dairy farms and it causes foodborne gastroenteritis by producing several toxins. This study is aimed at characterizing virulence determinants of B. cereus from cow‟s raw milk. A total of 400 raw milk samples were collected in Fort Hare Dairy Trust and Middledrift Dairy Farm; and cultured on Polymyxin pyruvate Egg-Yolk Mannitol Bromothymol Agar (PEMBA) for 48 hours at 37°C. DNA was isolated from the isolates and 16S rDNA was amplified and sent to Central Analytical Laboratory for sequencing. The gyrB gene of B. cereus was also used to confirm the identity of the isolates. Antibiotic susceptibility profiles of the isolates together with virulence genes were investigated. Multilocus Sequence typing was used to investigate the genetic relatedness of some selected isolates. Furthermore, spores of the isolates were produced, harvested and their concentrations determined. All (100%) of the isolates were identified as having a 96-99% similarity to B. cereus, B. thuringiensis and B. anthracis using sequencing; while gyrB gene was observed in all (100%) of the isolates. Three virulence genes nheA, nheB, nheC encoding for non haemolysin enterotoxin were amplified in all (100%) the isolates. All (100%) of the isolates were susceptible to doxycycline, gentamycin, tetracycline, ciprofloxacin, chloramphenicol and streptomycin. Resistance to rifampicin and penicillin G was predominant with equal rate of 100%, while susceptibility to erythromycin, clindamycin and doxycycline ranged from 60% to 100%. The selected isolates were related and are descendants of the same ancestor. All (100%) the isolates produced spores. The B. cereus isolates contain virulence genes, has multiple antibiotic drug resistance and produce spores, which poses a health risk to the public and cannot be used as probiotics.
- Full Text:
- Date Issued: 2015
- Authors: Lukanji, Zinathi , Ndip, R N
- Date: 2015
- Subjects: Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11296 , http://hdl.handle.net/10353/d1021284 , Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Description: Bacillus cereus is a group of ubiquitous facultative anaerobic spore forming Gram-positive rods commonly found in soil. It has been detected and implicated in several contaminated food products and raw milk in dairy farms and it causes foodborne gastroenteritis by producing several toxins. This study is aimed at characterizing virulence determinants of B. cereus from cow‟s raw milk. A total of 400 raw milk samples were collected in Fort Hare Dairy Trust and Middledrift Dairy Farm; and cultured on Polymyxin pyruvate Egg-Yolk Mannitol Bromothymol Agar (PEMBA) for 48 hours at 37°C. DNA was isolated from the isolates and 16S rDNA was amplified and sent to Central Analytical Laboratory for sequencing. The gyrB gene of B. cereus was also used to confirm the identity of the isolates. Antibiotic susceptibility profiles of the isolates together with virulence genes were investigated. Multilocus Sequence typing was used to investigate the genetic relatedness of some selected isolates. Furthermore, spores of the isolates were produced, harvested and their concentrations determined. All (100%) of the isolates were identified as having a 96-99% similarity to B. cereus, B. thuringiensis and B. anthracis using sequencing; while gyrB gene was observed in all (100%) of the isolates. Three virulence genes nheA, nheB, nheC encoding for non haemolysin enterotoxin were amplified in all (100%) the isolates. All (100%) of the isolates were susceptible to doxycycline, gentamycin, tetracycline, ciprofloxacin, chloramphenicol and streptomycin. Resistance to rifampicin and penicillin G was predominant with equal rate of 100%, while susceptibility to erythromycin, clindamycin and doxycycline ranged from 60% to 100%. The selected isolates were related and are descendants of the same ancestor. All (100%) the isolates produced spores. The B. cereus isolates contain virulence genes, has multiple antibiotic drug resistance and produce spores, which poses a health risk to the public and cannot be used as probiotics.
- Full Text:
- Date Issued: 2015
Microbiological analyses of beef slaughtering process and meat safety knowledge of handlers at selected high and low thoughput abattoirs
- Authors: Nyamakwere, Faith
- Date: 2015
- Subjects: Slaughtering and slaughter-houses -- South Africa -- Eastern Cape Meat industry and trade -- Quality control Beef -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2238 , vital:27712
- Description: The objective of the study was to evaluate meat hygiene practices among meat handlers and microbiological analysis of beef carcasses, slaughtermen hands, equipment and water from low throughput (LTA) and high throughput (HTA) abattoirs in the Eastern Cape Province of South Africa. In stage one of the study, the level of knowledge, practices and attitudes towards meat safety and personal hygiene of slaughtermen were assessed. Forty workers directly involve in beef cattle slaughtering process were surveyed. Data were collected using a structured questionnaire, it contained questions on some of the most important meat safety cues such as knowledge, attitudes, personal hygiene and handling practices. Cross tabulation and Chi-square Test of Association were performed to examine the relationships between the demographic information and the knowledge regarding meat safety using the Statistical Package for Social Sciences (SPSS) version 22. Overall, a significant adherence to basic hygiene practices and a satisfactory level of knowledge were observed. Workers from a HTA yielded comparatively better statistically significant scores. Moreover, knowledge and practices of respondents were significantly associated (P<0.05) due to educational level and professional training. The second stage of the study involved evaluating the level to which cattle carcass, equipment, slaughtermen hands and water were contaminated with Enterobacteriaceae, Salmonella, Escherichia coli and aerobic colony counts (ACC) at different stages during the slaughter process (skinning, evisceration, carcass slitting, inspection, washing and packing). Cattle carcasses were sampled at four sites (rump, neck, flank and brisket) from a LTA (n= 240) and HTA (n= 384) abattoirs. Using conventional biochemical tests, HTA yielded significantly (P<0.05) higher ACC (5.2 log CFU/cm2), E. coli (2.6 log CFU/cm2) and Enterobacteriaceae (2.9 log CFU/cm2) carcass mean scores than LTA after skinning and evisceration. Specific abattoir hygiene differences were noted from washed and chilled carcasses. Salmonella was not detected across all sampled slaughter process stages. In addition, the equipment had bacterial load ranging from 10 to 4 CFU/cm2 for LTA, whereas in HTA this was found to be 7 to 3 CFU/cm2. The bacterial counts for slaughtermen hands were estimated to be 15 to 8 CFU/cm2 in HTA and 10 to 5 CFU/cm2 in LTA. Overall, slaughtermen hands and equipment in the dirty area (skinning and evisceration) yielded more bacterial counts compared to those in the clean area (slitting, inspection, washing and packing) from both abattoirs. For all the sampled carcasses, equipment and slaughtermen hands, HTA yielded comparatively higher (P<0.05) bacterial counts than the LTA. Although the results showed a significant adherence to basic hygiene practices, some aspects such as routine medical examination, health certificates and professional training of slaughtermen still need to be improved. Therefore, these findings show that slaughtermen, equipment and water can be sources of contamination during the slaughter process.
- Full Text:
- Date Issued: 2015
- Authors: Nyamakwere, Faith
- Date: 2015
- Subjects: Slaughtering and slaughter-houses -- South Africa -- Eastern Cape Meat industry and trade -- Quality control Beef -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2238 , vital:27712
- Description: The objective of the study was to evaluate meat hygiene practices among meat handlers and microbiological analysis of beef carcasses, slaughtermen hands, equipment and water from low throughput (LTA) and high throughput (HTA) abattoirs in the Eastern Cape Province of South Africa. In stage one of the study, the level of knowledge, practices and attitudes towards meat safety and personal hygiene of slaughtermen were assessed. Forty workers directly involve in beef cattle slaughtering process were surveyed. Data were collected using a structured questionnaire, it contained questions on some of the most important meat safety cues such as knowledge, attitudes, personal hygiene and handling practices. Cross tabulation and Chi-square Test of Association were performed to examine the relationships between the demographic information and the knowledge regarding meat safety using the Statistical Package for Social Sciences (SPSS) version 22. Overall, a significant adherence to basic hygiene practices and a satisfactory level of knowledge were observed. Workers from a HTA yielded comparatively better statistically significant scores. Moreover, knowledge and practices of respondents were significantly associated (P<0.05) due to educational level and professional training. The second stage of the study involved evaluating the level to which cattle carcass, equipment, slaughtermen hands and water were contaminated with Enterobacteriaceae, Salmonella, Escherichia coli and aerobic colony counts (ACC) at different stages during the slaughter process (skinning, evisceration, carcass slitting, inspection, washing and packing). Cattle carcasses were sampled at four sites (rump, neck, flank and brisket) from a LTA (n= 240) and HTA (n= 384) abattoirs. Using conventional biochemical tests, HTA yielded significantly (P<0.05) higher ACC (5.2 log CFU/cm2), E. coli (2.6 log CFU/cm2) and Enterobacteriaceae (2.9 log CFU/cm2) carcass mean scores than LTA after skinning and evisceration. Specific abattoir hygiene differences were noted from washed and chilled carcasses. Salmonella was not detected across all sampled slaughter process stages. In addition, the equipment had bacterial load ranging from 10 to 4 CFU/cm2 for LTA, whereas in HTA this was found to be 7 to 3 CFU/cm2. The bacterial counts for slaughtermen hands were estimated to be 15 to 8 CFU/cm2 in HTA and 10 to 5 CFU/cm2 in LTA. Overall, slaughtermen hands and equipment in the dirty area (skinning and evisceration) yielded more bacterial counts compared to those in the clean area (slitting, inspection, washing and packing) from both abattoirs. For all the sampled carcasses, equipment and slaughtermen hands, HTA yielded comparatively higher (P<0.05) bacterial counts than the LTA. Although the results showed a significant adherence to basic hygiene practices, some aspects such as routine medical examination, health certificates and professional training of slaughtermen still need to be improved. Therefore, these findings show that slaughtermen, equipment and water can be sources of contamination during the slaughter process.
- Full Text:
- Date Issued: 2015
Ni(II) and Pb(II) dithiocarbamate complexes as precursors for the synthesis of HDA-capped NiS and PbS nanoparticles
- Authors: Chintso, Thobani
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11349 , http://hdl.handle.net/10353/d1020201
- Description: Ni(II) and Pb(II) dithiocarbamate complexes were synthesized and characterized by elemental analysis, UV-Vis, FTIR and TGA and some of the Ni(II) complexes and one Pb(II) were further analyzed by 1H-NMR and 13C-NMR spectroscopy. Generally all the dithiocarbamate ligands are soluble in water while the complexes were soluble mostly in solvents such as chloroform, toluene, DMSO and DCM. Based on the elemental analysis, the dithiocarbamate complexes are formulated as four coordinate (tetrahedral or square planar) compounds. However, the FTIR showed that each of the dithiocarbamate ligands acted as bidentate ligand through two sulfur atoms. The TGA of the most complexes showed one major decomposition step to give respective metal sulfide above 200 oC. In this research project, dithiocarbamate complexes were used as single source precursor for the synthesis of metal sulfide nanoparticles. We studied the optical and structural properties of metal sulfide nanoparticles using UV-Vis, photoluminescence (PL), powder X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The absorption spectra of the metal sulfide nanoparticles are blue shifted in respect to bulk material and they also showed broad emission. The XRD of the NiS nanoparticles were indexed to the cubic and rhombohedral phase, with crystallite sizes of 15 - 18 nm. The XRD of PbS nanoparticles were indexed to the face centered cubic and cubic rock salts, with the crystallite sizes 12 - 18 nm. The TEM images of the metal sulfide nanoparticles showed particles with spherical and rectangular shapes with crystallite sizes 4 - 35 nm.
- Full Text:
- Date Issued: 2015
- Authors: Chintso, Thobani
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11349 , http://hdl.handle.net/10353/d1020201
- Description: Ni(II) and Pb(II) dithiocarbamate complexes were synthesized and characterized by elemental analysis, UV-Vis, FTIR and TGA and some of the Ni(II) complexes and one Pb(II) were further analyzed by 1H-NMR and 13C-NMR spectroscopy. Generally all the dithiocarbamate ligands are soluble in water while the complexes were soluble mostly in solvents such as chloroform, toluene, DMSO and DCM. Based on the elemental analysis, the dithiocarbamate complexes are formulated as four coordinate (tetrahedral or square planar) compounds. However, the FTIR showed that each of the dithiocarbamate ligands acted as bidentate ligand through two sulfur atoms. The TGA of the most complexes showed one major decomposition step to give respective metal sulfide above 200 oC. In this research project, dithiocarbamate complexes were used as single source precursor for the synthesis of metal sulfide nanoparticles. We studied the optical and structural properties of metal sulfide nanoparticles using UV-Vis, photoluminescence (PL), powder X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The absorption spectra of the metal sulfide nanoparticles are blue shifted in respect to bulk material and they also showed broad emission. The XRD of the NiS nanoparticles were indexed to the cubic and rhombohedral phase, with crystallite sizes of 15 - 18 nm. The XRD of PbS nanoparticles were indexed to the face centered cubic and cubic rock salts, with the crystallite sizes 12 - 18 nm. The TEM images of the metal sulfide nanoparticles showed particles with spherical and rectangular shapes with crystallite sizes 4 - 35 nm.
- Full Text:
- Date Issued: 2015
Perceptions of indigenous people regarding mental illness at Cacadu District in the Eastern Cape Province of South Africa
- Authors: Tilolo, Lwazi Romeo
- Date: 2015
- Subjects: Indigenous peoples -- Mental health -- South Africa -- Eastern Cape Cultural psychiatry -- South Africa -- Eastern Cape Healers -- South Africa -- Eastern Cape , Ethnopsychology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2677 , vital:27983
- Description: Indigenous people tend to consult traditional healers when a family member manifests change in behaviour, whilst conventional treatment disregards spirituality when preserving mental health. The aim of the study was to explore the perceptions of indigenous people and the role of traditional healers in the management of mentally ill persons within the Cacadu District in the Eastern Cape Province of South Africa. The study has adopted a qualitative approach which was exploratory and descriptive in nature. The sample consisted of nine relatives of mental health care users (MHCUs) and six traditional healers. Ethical principles were also taken into consideration by the researcher during the process of conducting the study. Data were collected from two strata, namely, relatives of the MHCUs and the traditional healers and an interview guide was used to conduct in-depth face-to-face interviews. Data were analysed using Tesch’s method of data analysis. Four themes emerged from the data categories and sub categories were identified. According to the themes participants indicated the negative impact of mental illness; as a result they portrayed great desperation regarding the means of accessing a cure for mental illness. Some participants showed insufficient knowledge regarding mental illness and had different perceptions and beliefs regarding the origin of mental illness. Available literature was used to emphasise and support the views that were expressed by both traditional healers and relatives of MHCUs. It has been highlighted from this study that indigenous people of Cacadu District view mental illness as spiritual in origin but they include Western medication for the benefit of the mentally ill. In addition, the relatives of the MHCUs highlighted the economic burden as the major problem that results from mental illness.
- Full Text:
- Date Issued: 2015
- Authors: Tilolo, Lwazi Romeo
- Date: 2015
- Subjects: Indigenous peoples -- Mental health -- South Africa -- Eastern Cape Cultural psychiatry -- South Africa -- Eastern Cape Healers -- South Africa -- Eastern Cape , Ethnopsychology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2677 , vital:27983
- Description: Indigenous people tend to consult traditional healers when a family member manifests change in behaviour, whilst conventional treatment disregards spirituality when preserving mental health. The aim of the study was to explore the perceptions of indigenous people and the role of traditional healers in the management of mentally ill persons within the Cacadu District in the Eastern Cape Province of South Africa. The study has adopted a qualitative approach which was exploratory and descriptive in nature. The sample consisted of nine relatives of mental health care users (MHCUs) and six traditional healers. Ethical principles were also taken into consideration by the researcher during the process of conducting the study. Data were collected from two strata, namely, relatives of the MHCUs and the traditional healers and an interview guide was used to conduct in-depth face-to-face interviews. Data were analysed using Tesch’s method of data analysis. Four themes emerged from the data categories and sub categories were identified. According to the themes participants indicated the negative impact of mental illness; as a result they portrayed great desperation regarding the means of accessing a cure for mental illness. Some participants showed insufficient knowledge regarding mental illness and had different perceptions and beliefs regarding the origin of mental illness. Available literature was used to emphasise and support the views that were expressed by both traditional healers and relatives of MHCUs. It has been highlighted from this study that indigenous people of Cacadu District view mental illness as spiritual in origin but they include Western medication for the benefit of the mentally ill. In addition, the relatives of the MHCUs highlighted the economic burden as the major problem that results from mental illness.
- Full Text:
- Date Issued: 2015
Phytochemical analysis and antibacterial properties of aqueous and ethanol extracts of Brachylaena elliptica (Thurb.) dc. and Brachylaena ilicifolia (Lam.) Phill & Schweick
- Authors: Sagbo, Idowu Jonas
- Date: 2015
- Subjects: Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11297 , http://hdl.handle.net/10353/d1021289 , Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Description: Resistance of human pathogenic bacterial strains results in selective pressure against known antibiotic. However, plant derived compounds that possess antibacterial potential are currently being investigated for treatment of wound infections in diabetic patients as they are inexpensive and non-toxic. Hence, this dissertation was designed to evaluate two medicinal plants (Brachylaena elliptica and Brachylaena ilicifolia) traditionally used in the treatment of various diseases such as diabetes, and its secondary complications in diabetic patients. The in vitro antioxidant activity of both plants were evaluated using DPPH (1, 1-diphenylhydrazl), ferric reducing power, ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), NO (nitric oxide) and H2O2 (hydrogen peroxide) techniques. The antibacterial test and Minimum inhibitory concentration (MIC) was determined by agar dilution method against 5 bacteria strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogene, Proteus vulgaris and Proteus mirabilis) infecting wounds in diabetic patients using amoxicillin and ciprofloxacin as positive control. The phytochemical analyses were assessed using standard published methods. Identification of bioactive components in essential oils of both plants were assessed using GCMS. The aqueous and ethanol extracts of both plants were also evaluated to identify bioactive components using LC-MS. The results of the phytochemical analysis revealed the presence of phenols, tannins, flavanoids, flavanols, proanthocyanidins, saponins and alkaloids in both plants. Both plants indicated strong antioxidant activities which might be due to the presence of bioactive compounds. The aqueous and ethanol leaf extracts of both plants demonstrated appreciable broad spectrum activities against these wound pathogens with MIC ranging between 5 and 0.3 mg/ml. The GC-MS analysis of the essential oils of both plants revealed the presence of monoterpenes, oxygenated sesquiterpenes, phenolics and esters. The LC-MS analysis of the aqueous and ethanol leaf extracts of both plants showed that both plants are rich in alkaloids, terpenes, terpenoids, monoterpernoids, and flavanoids. Conclusively, this study has partially justified the ethnomedicinal use of B. elliptica and B.licifolia leaves for the treatment of various diseases, including diabetes and wound infections caused by bacteria in diabetic patients. These may be attributed to the presence of antioxidant compound such as phenols, flavanoids, saponins, tannins, alkaloids and other phytochemical compounds.
- Full Text:
- Date Issued: 2015
- Authors: Sagbo, Idowu Jonas
- Date: 2015
- Subjects: Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11297 , http://hdl.handle.net/10353/d1021289 , Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Description: Resistance of human pathogenic bacterial strains results in selective pressure against known antibiotic. However, plant derived compounds that possess antibacterial potential are currently being investigated for treatment of wound infections in diabetic patients as they are inexpensive and non-toxic. Hence, this dissertation was designed to evaluate two medicinal plants (Brachylaena elliptica and Brachylaena ilicifolia) traditionally used in the treatment of various diseases such as diabetes, and its secondary complications in diabetic patients. The in vitro antioxidant activity of both plants were evaluated using DPPH (1, 1-diphenylhydrazl), ferric reducing power, ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), NO (nitric oxide) and H2O2 (hydrogen peroxide) techniques. The antibacterial test and Minimum inhibitory concentration (MIC) was determined by agar dilution method against 5 bacteria strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogene, Proteus vulgaris and Proteus mirabilis) infecting wounds in diabetic patients using amoxicillin and ciprofloxacin as positive control. The phytochemical analyses were assessed using standard published methods. Identification of bioactive components in essential oils of both plants were assessed using GCMS. The aqueous and ethanol extracts of both plants were also evaluated to identify bioactive components using LC-MS. The results of the phytochemical analysis revealed the presence of phenols, tannins, flavanoids, flavanols, proanthocyanidins, saponins and alkaloids in both plants. Both plants indicated strong antioxidant activities which might be due to the presence of bioactive compounds. The aqueous and ethanol leaf extracts of both plants demonstrated appreciable broad spectrum activities against these wound pathogens with MIC ranging between 5 and 0.3 mg/ml. The GC-MS analysis of the essential oils of both plants revealed the presence of monoterpenes, oxygenated sesquiterpenes, phenolics and esters. The LC-MS analysis of the aqueous and ethanol leaf extracts of both plants showed that both plants are rich in alkaloids, terpenes, terpenoids, monoterpernoids, and flavanoids. Conclusively, this study has partially justified the ethnomedicinal use of B. elliptica and B.licifolia leaves for the treatment of various diseases, including diabetes and wound infections caused by bacteria in diabetic patients. These may be attributed to the presence of antioxidant compound such as phenols, flavanoids, saponins, tannins, alkaloids and other phytochemical compounds.
- Full Text:
- Date Issued: 2015
Prevalence and antibiogram of some swine associated Shiga toxin producing Escherichia coli Serogroups and Salmonella species in Nkonkobe Municipality, Eastern Cape Province, South Africa
- Authors: Iwu, Chinwe Juliana
- Date: 2015
- Subjects: Escherichia coli -- South Africa -- Eastern Cape , Salmonella infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape , Escherichia coli infections -- South Africa -- Eastern Cape , Water quality management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11294 , http://hdl.handle.net/10353/d1021273 , Escherichia coli -- South Africa -- Eastern Cape , Salmonella infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape , Escherichia coli infections -- South Africa -- Eastern Cape , Water quality management -- South Africa -- Eastern Cape
- Description: Gastrointestinal illnesses have continually become a global public health issue. Exposure to zoonotic food borne pathogens such as Salmonella and diarrhoegenic E. coli either by direct or indirect contact through the consumption of food producing animals is likely an important mode of infection to humans. More so, the use of antibiotics in farm animals similar to those used in humans can select for resistance in bacteria frequently harboured by them. These resistant strains can be passed on to humans through contaminated meat products and water leading to resistant infections with consequences such as prolonged illnesses, treatment failures, and increased morbidity and mortality. In animals, these can lead to reduced productivity. Monitoring the level of resistance among bacteria from animal isolates will help in generating data that could be used to create awareness of their presence in the environment and aid in preventing a potential epidemic in the community. In this study, we investigated the prevalence and antimicrobial resistance profile of Escherichia coli serogroups and Salmonella species in faecal samples collected from pigs in Nkonkobe Municipality in the Eastern Cape Province, South Africa between April – July, 2014. A total of 310 presumptive Shiga toxin producing Escherichia coli (STEC) were confirmed as E. coli spp using polymerase chain reaction (PCR) technique by amplification of the uidA gene, out of which 179 (58%) were confirmed positive. Approximately, serogrougs O157:H7, O145 and O26 made up 24% (n=43), 8% (n=14) and 20% (n=35) of the E. coli population respectively. Only E. coli O26 was positive for stx2 gene in 31% of the isolates harbouring the gene, while the other serogroups were non-pathogenic. Susceptibility of the isolates to 18 antibiotics was carried out in vitro by the standardized agar disc-diffusion method. All the isolates were susceptible to imipenem. Similarly, a relatively high susceptibility was observed in norfloxacin (83-100%), ciprofloxacin (63-100%), gentamycin (77-100%), and chloramphenicol (77-100%). However, all the isolates were resistant to tetracycline and its long acting counterpart oxytetracycline. Resistances observed against other antimicrobials are as follows: ampicillin (84-91%), streptomycin (14-100%), erythromycin (91-100%), ceftazidime (35%). Multiple antimicrobial resistance patterns and indices ranged from 3 to 12 and 0.2 to 0.7 to respectively. Genes encoding resistances to ampicillin (ampC), streptomycin (strA) and tetracycline (tetA) were frequently detected in 50-100%, 22-29% and 40-86% of the resistant isolates respectively. In the other arm of the dissertation, two hundred and fifty eight presumptive isolates of Salmonella were recovered from the faecal samples of pigs. Specific primers targeting serogroups A, B, C1, C2, and D were used to delineate the isolates into different serogroups using PCR. Only serogroup A (n=48) was detected. These isolates were examined for antimicrobial susceptibility by disc diffusion method using 18 antibiotics. The results showed that a large proportion of the isolates were resistant to tetracycline (100%), oxytetracycline (100%), ampicillin (75%), sulphamethoxazole/trimethoprim (75%) and streptomycin (75%). Majority of the isolates exhibited multidrug resistances with the predominant multiple antibiotic resistance (MAR) phenotype being against eleven antibiotics. A high multiple antibiotic resistance (MAR) index in a range of 0.3- 0.6 was observed. The incidence of genes encoding resistance against tetracycline (tetA), streptomycin (stra), and ampicillin (ampC) were 54%, 44% and 61% respectively. These findings reveal that pigs within the Nkonkobe Municipality in the Eastern Cape Province could harbour Shiga toxins and multidrug resistant serogroups of E. coli as well as resistant Salmonella which could be transmitted to humans through the food chain. To ensure public health safety, continuous monitoring and sufficient sanitation in swine industries must be ensured.
- Full Text:
- Date Issued: 2015
- Authors: Iwu, Chinwe Juliana
- Date: 2015
- Subjects: Escherichia coli -- South Africa -- Eastern Cape , Salmonella infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape , Escherichia coli infections -- South Africa -- Eastern Cape , Water quality management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11294 , http://hdl.handle.net/10353/d1021273 , Escherichia coli -- South Africa -- Eastern Cape , Salmonella infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape , Escherichia coli infections -- South Africa -- Eastern Cape , Water quality management -- South Africa -- Eastern Cape
- Description: Gastrointestinal illnesses have continually become a global public health issue. Exposure to zoonotic food borne pathogens such as Salmonella and diarrhoegenic E. coli either by direct or indirect contact through the consumption of food producing animals is likely an important mode of infection to humans. More so, the use of antibiotics in farm animals similar to those used in humans can select for resistance in bacteria frequently harboured by them. These resistant strains can be passed on to humans through contaminated meat products and water leading to resistant infections with consequences such as prolonged illnesses, treatment failures, and increased morbidity and mortality. In animals, these can lead to reduced productivity. Monitoring the level of resistance among bacteria from animal isolates will help in generating data that could be used to create awareness of their presence in the environment and aid in preventing a potential epidemic in the community. In this study, we investigated the prevalence and antimicrobial resistance profile of Escherichia coli serogroups and Salmonella species in faecal samples collected from pigs in Nkonkobe Municipality in the Eastern Cape Province, South Africa between April – July, 2014. A total of 310 presumptive Shiga toxin producing Escherichia coli (STEC) were confirmed as E. coli spp using polymerase chain reaction (PCR) technique by amplification of the uidA gene, out of which 179 (58%) were confirmed positive. Approximately, serogrougs O157:H7, O145 and O26 made up 24% (n=43), 8% (n=14) and 20% (n=35) of the E. coli population respectively. Only E. coli O26 was positive for stx2 gene in 31% of the isolates harbouring the gene, while the other serogroups were non-pathogenic. Susceptibility of the isolates to 18 antibiotics was carried out in vitro by the standardized agar disc-diffusion method. All the isolates were susceptible to imipenem. Similarly, a relatively high susceptibility was observed in norfloxacin (83-100%), ciprofloxacin (63-100%), gentamycin (77-100%), and chloramphenicol (77-100%). However, all the isolates were resistant to tetracycline and its long acting counterpart oxytetracycline. Resistances observed against other antimicrobials are as follows: ampicillin (84-91%), streptomycin (14-100%), erythromycin (91-100%), ceftazidime (35%). Multiple antimicrobial resistance patterns and indices ranged from 3 to 12 and 0.2 to 0.7 to respectively. Genes encoding resistances to ampicillin (ampC), streptomycin (strA) and tetracycline (tetA) were frequently detected in 50-100%, 22-29% and 40-86% of the resistant isolates respectively. In the other arm of the dissertation, two hundred and fifty eight presumptive isolates of Salmonella were recovered from the faecal samples of pigs. Specific primers targeting serogroups A, B, C1, C2, and D were used to delineate the isolates into different serogroups using PCR. Only serogroup A (n=48) was detected. These isolates were examined for antimicrobial susceptibility by disc diffusion method using 18 antibiotics. The results showed that a large proportion of the isolates were resistant to tetracycline (100%), oxytetracycline (100%), ampicillin (75%), sulphamethoxazole/trimethoprim (75%) and streptomycin (75%). Majority of the isolates exhibited multidrug resistances with the predominant multiple antibiotic resistance (MAR) phenotype being against eleven antibiotics. A high multiple antibiotic resistance (MAR) index in a range of 0.3- 0.6 was observed. The incidence of genes encoding resistance against tetracycline (tetA), streptomycin (stra), and ampicillin (ampC) were 54%, 44% and 61% respectively. These findings reveal that pigs within the Nkonkobe Municipality in the Eastern Cape Province could harbour Shiga toxins and multidrug resistant serogroups of E. coli as well as resistant Salmonella which could be transmitted to humans through the food chain. To ensure public health safety, continuous monitoring and sufficient sanitation in swine industries must be ensured.
- Full Text:
- Date Issued: 2015
Regional differentiation of three goatfishes (Parupeneus Spp.) within the Western Indian Ocean
- Springbok–Njokweni, Nosiphiwo
- Authors: Springbok–Njokweni, Nosiphiwo
- Date: 2015
- Subjects: Marine fishes -- South Africa , Mitochondrial DNA -- South Africa , Mullidae -- South Africa , Biogeography -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Zoology)
- Identifier: vital:11798 , http://hdl.handle.net/10353/d1020262 , Marine fishes -- South Africa , Mitochondrial DNA -- South Africa , Mullidae -- South Africa , Biogeography -- South Africa
- Description: Goatfishes inhabit inshore reefs and corals and are commercially important across their distribution in the Western Indian Ocean (WIO). The biogeography of these species in the WIO has not been explored with regards to their levels of diversity and relationships among regions. The genetic connectivity and differentiation of three goatfishes of the genus Parupeneus (P. barberinus, P. macronemus and P. rubescens) was studied using two mitochondrial genes (ND2 and 16S rRNA) and one nuclear gene (RAG1) using specimens from East and southern Africa, islands around the Mascarene plateau, Oman, Maldives and the Red Sea. Haplotype diversities, networks and AMOVA were used to measure genetic variance among localities and defined regional groups. There were high haplotype (HD > 0.9) and low nucleotide diversities (< 0.006) among all species for all gene regions, suggesting high levels of genetic differentiation among different areas, except for the mtDNA 16S data for P. macronemus and P. rubescens. For all three species, the FST population pairwise values revealed significant differentiation in all datasets for most population pairwise comparisons with the Maldives and genetic connectivity with haplotypes being shared among other localities. The 16S and RAG1, AMOVA for P. barberinus revealed a significant (P < 0.05) strong genetic structure among groups, for example P = 0.00 was estimated in the 16S data for four groups (the Maldives, WIO islands, Kenya and eastern mainland). This study found evidence for regional differentiation within the WIO for these three species supporting the presence of genetic breaks among areas. This differentiation could be either due to the historical isolation among areas or due to geographic and oceanic barriers such as the Mascarene Plateau and the Agulhas Current eddies in the Mozambique Channel. The effects of oceanographic features and physical barriers in the species distribution range and the dispersal potential based on the life history features of the species can have an influence on the genetic structuring of a population. It is also important to note that the length of the pelagic larval phase is just one factor affecting dispersal in marine organisms that can also explain the difference in genetic population structure. Unfortunately there is no specific information on the larval dispersal of these three goatfish. Therefore, studies are needed to be conducted on the specific biology and life history strategies of each Parupeneus species. These results suggest the importance of other factors, such as currents, and larval retention that may cause strong differentiation. These factors should also be considered when observing larval dispersal and its effect on population genetic structure. This study support the hypotheses that physical factors, processes (geographic barriers and oceanographic characteristics) and life history parameters need to be studied to understand the genetic differentiation of these Parupeneus reef fishes.
- Full Text:
- Date Issued: 2015
- Authors: Springbok–Njokweni, Nosiphiwo
- Date: 2015
- Subjects: Marine fishes -- South Africa , Mitochondrial DNA -- South Africa , Mullidae -- South Africa , Biogeography -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Zoology)
- Identifier: vital:11798 , http://hdl.handle.net/10353/d1020262 , Marine fishes -- South Africa , Mitochondrial DNA -- South Africa , Mullidae -- South Africa , Biogeography -- South Africa
- Description: Goatfishes inhabit inshore reefs and corals and are commercially important across their distribution in the Western Indian Ocean (WIO). The biogeography of these species in the WIO has not been explored with regards to their levels of diversity and relationships among regions. The genetic connectivity and differentiation of three goatfishes of the genus Parupeneus (P. barberinus, P. macronemus and P. rubescens) was studied using two mitochondrial genes (ND2 and 16S rRNA) and one nuclear gene (RAG1) using specimens from East and southern Africa, islands around the Mascarene plateau, Oman, Maldives and the Red Sea. Haplotype diversities, networks and AMOVA were used to measure genetic variance among localities and defined regional groups. There were high haplotype (HD > 0.9) and low nucleotide diversities (< 0.006) among all species for all gene regions, suggesting high levels of genetic differentiation among different areas, except for the mtDNA 16S data for P. macronemus and P. rubescens. For all three species, the FST population pairwise values revealed significant differentiation in all datasets for most population pairwise comparisons with the Maldives and genetic connectivity with haplotypes being shared among other localities. The 16S and RAG1, AMOVA for P. barberinus revealed a significant (P < 0.05) strong genetic structure among groups, for example P = 0.00 was estimated in the 16S data for four groups (the Maldives, WIO islands, Kenya and eastern mainland). This study found evidence for regional differentiation within the WIO for these three species supporting the presence of genetic breaks among areas. This differentiation could be either due to the historical isolation among areas or due to geographic and oceanic barriers such as the Mascarene Plateau and the Agulhas Current eddies in the Mozambique Channel. The effects of oceanographic features and physical barriers in the species distribution range and the dispersal potential based on the life history features of the species can have an influence on the genetic structuring of a population. It is also important to note that the length of the pelagic larval phase is just one factor affecting dispersal in marine organisms that can also explain the difference in genetic population structure. Unfortunately there is no specific information on the larval dispersal of these three goatfish. Therefore, studies are needed to be conducted on the specific biology and life history strategies of each Parupeneus species. These results suggest the importance of other factors, such as currents, and larval retention that may cause strong differentiation. These factors should also be considered when observing larval dispersal and its effect on population genetic structure. This study support the hypotheses that physical factors, processes (geographic barriers and oceanographic characteristics) and life history parameters need to be studied to understand the genetic differentiation of these Parupeneus reef fishes.
- Full Text:
- Date Issued: 2015
Socio-economic factors influencing the adoption of in-field rainwater harvesting technololgy for enhancing household food security by small holder farmers in the Nkonkobe Municipality, Eastern Cape Province
- Shange, Nomfundo Sinethemba Queen
- Authors: Shange, Nomfundo Sinethemba Queen
- Date: 2015
- Subjects: Farms, Small -- South Africa -- Eastern Cape , Water harvesting -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Water resources development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: http://hdl.handle.net/10353/734 , vital:26492 , Farms, Small -- South Africa -- Eastern Cape , Water harvesting -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Water resources development -- South Africa -- Eastern Cape
- Description: Infield rainwater harvesting (IRWH) technology has been used in arid and semi-arid parts of the world and promising results have been achieved in terms of increasing yield. The main aim of this study was to identify socio-economic factors determining the adoption of IRWH technology for enhancing household food security by smallholder farmers. The specific objectives were to assess the level of adoption of IRWH technology using descriptive statistics (mean, frequency and percentages). To determine socio-economic factors influencing adoption of IRWH technology, the binary logistic regression mode l was used. To determine whether adopters of IRWH technology are more food secure than non-adopters, the Household Dietary Diversity Score (HDDS) was used as a measure for household food security. For the same objective, to determine socio-economic factors that influence household food security, the binary logistic regression model was also used and adoption of IRWH technology became an independent variable. The study was conducted in Khayalethu, Guquka and Krwakrwa villages in Nkonkobe Municipality in the Eastern Cape Province (EC). The unit of analysis was the individual smallholder farmers practicing agriculture. The availability (accidental) and snowball sampling techniques were used to select 34, 23, 63 respondents from Khayalethu, Guquka and Krwakrwa villages respectively. Since they are non-random, these sampling methods are problematic because of sampling errors. Overall, a sample size of 120 smallholder farmers was targeted for the interviews. Primary and secondary data collected was coded and analysed using statistical package for social sciences (SPSS) version 21. Results were presented using graphs, pie charts and tables (including cross-tables). The descriptive results showed that adoption status of IRWH technology was low in these areas, with 79% not adopting the technology. Food insecurity was high amongst the non-adopters with 86%. On the basis of descriptive analysis it can be concluded that any change in each one of the significant variables can significantly influence the probability of adopting IRWH technology and household food security. The results from the logistic regression model for the incidence of adoption revealed that 6 out of 16 variables were significant, three at 1% (access to extension services, access to information and farmers’ perception towards the IRWH technology); one at 5% (access to market) and two at 10% (access to hired labour and farm income). For the incidence of household food security, out of 17 variables, 6 were significant, three at 1% (adoption of the IRWH technology, access to extension services and farmers’ perception towards the IRWH technology); two at 5% (access to hired labour and household income) and one at 10% (household size). The empirical findings of this study indicate that there are socio-economic factors influencing adoption of IRWH technology and household food security amongst smallholder farmers. This study recommends that the government should provide extension officers and research stations with the capacity, support and physical means to expose smallholder farmers to the IRWH technology through demonstrations and trainings. The government can also introduce agricultural finance institutions in rural areas to assist the rural smallholder farmers to increase their access to credit. Further, it is recommended that smallholder farmers can expand to the communal croplands in order to gain more land size and work as a co-operative or as an association to ease labour constraints.
- Full Text:
- Date Issued: 2015
- Authors: Shange, Nomfundo Sinethemba Queen
- Date: 2015
- Subjects: Farms, Small -- South Africa -- Eastern Cape , Water harvesting -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Water resources development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: http://hdl.handle.net/10353/734 , vital:26492 , Farms, Small -- South Africa -- Eastern Cape , Water harvesting -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Water resources development -- South Africa -- Eastern Cape
- Description: Infield rainwater harvesting (IRWH) technology has been used in arid and semi-arid parts of the world and promising results have been achieved in terms of increasing yield. The main aim of this study was to identify socio-economic factors determining the adoption of IRWH technology for enhancing household food security by smallholder farmers. The specific objectives were to assess the level of adoption of IRWH technology using descriptive statistics (mean, frequency and percentages). To determine socio-economic factors influencing adoption of IRWH technology, the binary logistic regression mode l was used. To determine whether adopters of IRWH technology are more food secure than non-adopters, the Household Dietary Diversity Score (HDDS) was used as a measure for household food security. For the same objective, to determine socio-economic factors that influence household food security, the binary logistic regression model was also used and adoption of IRWH technology became an independent variable. The study was conducted in Khayalethu, Guquka and Krwakrwa villages in Nkonkobe Municipality in the Eastern Cape Province (EC). The unit of analysis was the individual smallholder farmers practicing agriculture. The availability (accidental) and snowball sampling techniques were used to select 34, 23, 63 respondents from Khayalethu, Guquka and Krwakrwa villages respectively. Since they are non-random, these sampling methods are problematic because of sampling errors. Overall, a sample size of 120 smallholder farmers was targeted for the interviews. Primary and secondary data collected was coded and analysed using statistical package for social sciences (SPSS) version 21. Results were presented using graphs, pie charts and tables (including cross-tables). The descriptive results showed that adoption status of IRWH technology was low in these areas, with 79% not adopting the technology. Food insecurity was high amongst the non-adopters with 86%. On the basis of descriptive analysis it can be concluded that any change in each one of the significant variables can significantly influence the probability of adopting IRWH technology and household food security. The results from the logistic regression model for the incidence of adoption revealed that 6 out of 16 variables were significant, three at 1% (access to extension services, access to information and farmers’ perception towards the IRWH technology); one at 5% (access to market) and two at 10% (access to hired labour and farm income). For the incidence of household food security, out of 17 variables, 6 were significant, three at 1% (adoption of the IRWH technology, access to extension services and farmers’ perception towards the IRWH technology); two at 5% (access to hired labour and household income) and one at 10% (household size). The empirical findings of this study indicate that there are socio-economic factors influencing adoption of IRWH technology and household food security amongst smallholder farmers. This study recommends that the government should provide extension officers and research stations with the capacity, support and physical means to expose smallholder farmers to the IRWH technology through demonstrations and trainings. The government can also introduce agricultural finance institutions in rural areas to assist the rural smallholder farmers to increase their access to credit. Further, it is recommended that smallholder farmers can expand to the communal croplands in order to gain more land size and work as a co-operative or as an association to ease labour constraints.
- Full Text:
- Date Issued: 2015
Stratigraphy and sedimentology of the Msikaba formation in KwaZulu Natal South Coast, South Africa
- Authors: Busakwe, Nolukholo Sinovuyo
- Date: 2015
- Subjects: Geology, Stratigraphic Sedimentology -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2501 , vital:27877
- Description: The Msikaba Formation is a Late Devonian fluvial and marine succession which outcrops from Hibberdene to Port Edward along the south coast of KwaZulu-Natal Province, South Africa. The Formation is composed of brownish conglomerate at the bottom and white-greyish quartz arenite sequence in the middle and mixed quartz-arenite with feldspathic sandstone in the upper sequence. Previous studies put more emphasis on the correlation of Msikaba Formation with the Natal Group and Cape Supergroup, whereas this study revised the stratigraphy, and also put new insight on the petrography, sedimentary facies, depositional environments and diagenesis of the Formation. The total stratigraphic section attains a thickness of 184 m at Margate area and 186 m at Port Edward area. The stratigraphy of Msikaba Formation is well exposed on the outcrops along the KwaZulu-Natal coastline. The stratigraphy is subdivided into 4 new members along Margate to Shelly beach section; namely Manaba Member, Uvongo Member, Mhlangeni Member and Shelly Beach Member from bottom upward. Twelve sedimentary facies were identified and the sedimentary facies were integrated into 4 facies association: Facies association 1 (Gmm+Sm) represents braided fluvial deposits, Facies association 2 (Gcm+St+Sp+Sl+Shb) represents tidal channel and tidal flat deposit, Facies association 3 (St+Sp+Sr+Sl) is result of shallow marine deposit and Facies association 4 (Sp+Sl+St+Sm) is a mixed marine and fluvial deposit. Each facies association represents a specific stratigraphic unit and were deposited in a specific sedimentary environment. Grain size analysis was conducted on seventeen thin sections and 500 grains were counted from each thin section. The sandstone grain size parameters of mean, sorting, skewness and kurtosis fell under the average of 0.75, 0.78, 0.4 and 1.2φ respectively. The results show that most of the grain size are coarse to medium grained throughout the study areas and sorting of the sandstones are moderate to poorly sorted. The cumulative frequency diagrams and bivariate plots show positive skewness and negative kurtosis, which indicate a high hydrodynamic environment. Modal composition analysis and petrography studies show that detrital components of the Msikaba Formation are dominated by monocrystalline quartz, feldspar (mostly K-feldspar) and lithic fragments of igneous and metamorphic rocks. The sandstones could be classified as quartz arenite, sub-arkosic sandstone and feldspathic litharenite; and the provenance analysis indicates that the sandstones were derived from craton interior, recycled or quartzose recycled sources which may derived from weathering and erosion of igneous and metamorphic rocks. Diagenetic processes of the Msikaba Formation have been passed through early, mid- and late diagenetic stages. Cementation, mineral conversion and compaction affect early diagenetic stage; authigenic minerals, quartz and feldspar overgrowth are presented in mid-diagenetic stage, whereas recrystallization, replacement, deformation and dissolution have been strongly affected late diagenetic stage. Microscopy, XRD and SEM-EDX studies have identified five types of cements including smectite clay, kaolinite, hematite, quartz and feldspar cements. Quartz cement, pore-filling and pore-lining clay are the major type of cements in the Msikaba Formation. Based on the lithology, sedimentary structure and facies variations, the Manaba Member was most probably deposited in a braided fluvial environment, the Uvongo Member was deposited in a tidal channel environment, the Mhlangeni Member was formed in shallow marine storm-influenced environment, whereas the Shelly Beach Member was represented mixed marine and fluvial environment. The sequence stratigraphy of Msikaba Formation constitutes a transgressive sequence from Manaba Member to Uvongo Member, whereas it ended as a regressive sequence from Mhlangeni Member to Shelly beach Member. The Msikaba Formation shows major differences with the Natal Group and Table Mountain Group (Cape Supergroup) in the lithology, stratigraphic sequence, sedimentary structures, facies system, palaeocurrent styles, fossil contents and depositional environments, which demonstrate that they are not the equivalent stratigraphic unit. Therefore, the Msikaba Formation is a separate, younger stratigraphic unit, and cannot correlate with the Natal Group and Table Mountain Group as suggested by previous researchers.
- Full Text:
- Date Issued: 2015
- Authors: Busakwe, Nolukholo Sinovuyo
- Date: 2015
- Subjects: Geology, Stratigraphic Sedimentology -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2501 , vital:27877
- Description: The Msikaba Formation is a Late Devonian fluvial and marine succession which outcrops from Hibberdene to Port Edward along the south coast of KwaZulu-Natal Province, South Africa. The Formation is composed of brownish conglomerate at the bottom and white-greyish quartz arenite sequence in the middle and mixed quartz-arenite with feldspathic sandstone in the upper sequence. Previous studies put more emphasis on the correlation of Msikaba Formation with the Natal Group and Cape Supergroup, whereas this study revised the stratigraphy, and also put new insight on the petrography, sedimentary facies, depositional environments and diagenesis of the Formation. The total stratigraphic section attains a thickness of 184 m at Margate area and 186 m at Port Edward area. The stratigraphy of Msikaba Formation is well exposed on the outcrops along the KwaZulu-Natal coastline. The stratigraphy is subdivided into 4 new members along Margate to Shelly beach section; namely Manaba Member, Uvongo Member, Mhlangeni Member and Shelly Beach Member from bottom upward. Twelve sedimentary facies were identified and the sedimentary facies were integrated into 4 facies association: Facies association 1 (Gmm+Sm) represents braided fluvial deposits, Facies association 2 (Gcm+St+Sp+Sl+Shb) represents tidal channel and tidal flat deposit, Facies association 3 (St+Sp+Sr+Sl) is result of shallow marine deposit and Facies association 4 (Sp+Sl+St+Sm) is a mixed marine and fluvial deposit. Each facies association represents a specific stratigraphic unit and were deposited in a specific sedimentary environment. Grain size analysis was conducted on seventeen thin sections and 500 grains were counted from each thin section. The sandstone grain size parameters of mean, sorting, skewness and kurtosis fell under the average of 0.75, 0.78, 0.4 and 1.2φ respectively. The results show that most of the grain size are coarse to medium grained throughout the study areas and sorting of the sandstones are moderate to poorly sorted. The cumulative frequency diagrams and bivariate plots show positive skewness and negative kurtosis, which indicate a high hydrodynamic environment. Modal composition analysis and petrography studies show that detrital components of the Msikaba Formation are dominated by monocrystalline quartz, feldspar (mostly K-feldspar) and lithic fragments of igneous and metamorphic rocks. The sandstones could be classified as quartz arenite, sub-arkosic sandstone and feldspathic litharenite; and the provenance analysis indicates that the sandstones were derived from craton interior, recycled or quartzose recycled sources which may derived from weathering and erosion of igneous and metamorphic rocks. Diagenetic processes of the Msikaba Formation have been passed through early, mid- and late diagenetic stages. Cementation, mineral conversion and compaction affect early diagenetic stage; authigenic minerals, quartz and feldspar overgrowth are presented in mid-diagenetic stage, whereas recrystallization, replacement, deformation and dissolution have been strongly affected late diagenetic stage. Microscopy, XRD and SEM-EDX studies have identified five types of cements including smectite clay, kaolinite, hematite, quartz and feldspar cements. Quartz cement, pore-filling and pore-lining clay are the major type of cements in the Msikaba Formation. Based on the lithology, sedimentary structure and facies variations, the Manaba Member was most probably deposited in a braided fluvial environment, the Uvongo Member was deposited in a tidal channel environment, the Mhlangeni Member was formed in shallow marine storm-influenced environment, whereas the Shelly Beach Member was represented mixed marine and fluvial environment. The sequence stratigraphy of Msikaba Formation constitutes a transgressive sequence from Manaba Member to Uvongo Member, whereas it ended as a regressive sequence from Mhlangeni Member to Shelly beach Member. The Msikaba Formation shows major differences with the Natal Group and Table Mountain Group (Cape Supergroup) in the lithology, stratigraphic sequence, sedimentary structures, facies system, palaeocurrent styles, fossil contents and depositional environments, which demonstrate that they are not the equivalent stratigraphic unit. Therefore, the Msikaba Formation is a separate, younger stratigraphic unit, and cannot correlate with the Natal Group and Table Mountain Group as suggested by previous researchers.
- Full Text:
- Date Issued: 2015
Synthesis and characterization of Ru(II) phenyl-3-indenylidene olefin metathesis type complexes
- Authors: Yalezo, Ntsikelelo
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11351 , http://hdl.handle.net/10353/d1021128
- Description: In this study, a series of Ru(II) phenyl-3-indenylidene complexes with general formula of [RuCl2(NHC)(Ind)(L)] (where L= triphenylphosphine, pyridine and NHC = five different types of N-heterocyclic carbene ligands), have been synthesized and characterized using FT-IR, UV-Vis, elementally analysis and melting/decomposition point. The N,N’-diarylimidazolinium chlorides have been used as N-heterocyclic carbene precursors and were synthesized from their corresponding N,N’-diarylformamidines and further characterized using 1H-NMR, 13C-NMR, FTIR and melting point determination. The infrared spectra of the N,N’-diarylimidazolinium chlorides show a quaternary nature (R2N=C+) with broad vibration band in region 3300-3400 cm-1. The disappearance of this vibration band in the infrared spectra of the ruthenium(II) complexes was used to confirm the coordination of the ligand to the ruthenium ions. The percentage analysis of carbon, hydrogen and nitrogen obtained corresponded with the calculated percentages of these atoms in the complexes with the slight difference of less than 1%. The electronic spectra of the complexes show three distinct absorption bands. The two bands are due to intraligand charge transfers transition assigned to π→π*, n→π* and third band is due to d-d transition, signifying the presence of the metal ion. The synthesized Ru(II) complexes did not show any of melting, however a change in colour was observed signifying the decomposition of the complexes.
- Full Text:
- Date Issued: 2015
- Authors: Yalezo, Ntsikelelo
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11351 , http://hdl.handle.net/10353/d1021128
- Description: In this study, a series of Ru(II) phenyl-3-indenylidene complexes with general formula of [RuCl2(NHC)(Ind)(L)] (where L= triphenylphosphine, pyridine and NHC = five different types of N-heterocyclic carbene ligands), have been synthesized and characterized using FT-IR, UV-Vis, elementally analysis and melting/decomposition point. The N,N’-diarylimidazolinium chlorides have been used as N-heterocyclic carbene precursors and were synthesized from their corresponding N,N’-diarylformamidines and further characterized using 1H-NMR, 13C-NMR, FTIR and melting point determination. The infrared spectra of the N,N’-diarylimidazolinium chlorides show a quaternary nature (R2N=C+) with broad vibration band in region 3300-3400 cm-1. The disappearance of this vibration band in the infrared spectra of the ruthenium(II) complexes was used to confirm the coordination of the ligand to the ruthenium ions. The percentage analysis of carbon, hydrogen and nitrogen obtained corresponded with the calculated percentages of these atoms in the complexes with the slight difference of less than 1%. The electronic spectra of the complexes show three distinct absorption bands. The two bands are due to intraligand charge transfers transition assigned to π→π*, n→π* and third band is due to d-d transition, signifying the presence of the metal ion. The synthesized Ru(II) complexes did not show any of melting, however a change in colour was observed signifying the decomposition of the complexes.
- Full Text:
- Date Issued: 2015
Synthesis, characterisation and evaluation of functionalized Lignocelluloses-clay nanocomposites for organic pollutant removal from water
- Authors: Mafukidze, Donovan M
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11356 , http://hdl.handle.net/10353/d1021296
- Description: PMPSgLig-NaMMT nanocomposites were prepared from methacryloxypropyltrimethoxysilane (MPS), lignocellulose and montmorillonite clay. The potential enhancement of organic pollutant adsorption capabilities of PMPSgLig-NaMMT nanocomposite from water through functionalization was investigated. PMPSgLig-NaMMT was functionalized by esterification and etherification using different methods so as to increase the surface hydrophobicity of the material and hence improve its compatibility with the target pollutants. Specific chemical routes specially tailored for PMPSgLig-NaMMT were established for functionalization mostly based on the common esterification (Fischer esterification) and etherification (Williamson‟s etherification) reactions. In the functionalization methods, factors such as pH environment, nanocomposite composition, nature of functionalization moiety, and use of or absence of solvents and their variations were studied. FT-IR, XRD, SEM and TGA were used to characterize the synthesized and functionalized nanoadsorbents. The techniques showed successful functionalization via esterification and etherification methods albeit to different extents, with clear retention of the material‟s original structure though there were signs of degradation with some methods. Characterization was supported by adsorption studies to validate implications and draw conclusions. The use of 1,10-phenathroline as a model organic pollutant in water in the adsorption studies showed that adsorbents conformed to monolayer adsorption following pseudo-second order kinetics for adsorption of organic pollutants accurately represented. Most importantly the studies revealed the significant impact of the nanocomposite composition on the overall absorbent performance. Adsorption studies also showed that functionalization via esterification methods gave rise to better adsorbents.
- Full Text:
- Date Issued: 2015
- Authors: Mafukidze, Donovan M
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11356 , http://hdl.handle.net/10353/d1021296
- Description: PMPSgLig-NaMMT nanocomposites were prepared from methacryloxypropyltrimethoxysilane (MPS), lignocellulose and montmorillonite clay. The potential enhancement of organic pollutant adsorption capabilities of PMPSgLig-NaMMT nanocomposite from water through functionalization was investigated. PMPSgLig-NaMMT was functionalized by esterification and etherification using different methods so as to increase the surface hydrophobicity of the material and hence improve its compatibility with the target pollutants. Specific chemical routes specially tailored for PMPSgLig-NaMMT were established for functionalization mostly based on the common esterification (Fischer esterification) and etherification (Williamson‟s etherification) reactions. In the functionalization methods, factors such as pH environment, nanocomposite composition, nature of functionalization moiety, and use of or absence of solvents and their variations were studied. FT-IR, XRD, SEM and TGA were used to characterize the synthesized and functionalized nanoadsorbents. The techniques showed successful functionalization via esterification and etherification methods albeit to different extents, with clear retention of the material‟s original structure though there were signs of degradation with some methods. Characterization was supported by adsorption studies to validate implications and draw conclusions. The use of 1,10-phenathroline as a model organic pollutant in water in the adsorption studies showed that adsorbents conformed to monolayer adsorption following pseudo-second order kinetics for adsorption of organic pollutants accurately represented. Most importantly the studies revealed the significant impact of the nanocomposite composition on the overall absorbent performance. Adsorption studies also showed that functionalization via esterification methods gave rise to better adsorbents.
- Full Text:
- Date Issued: 2015
The classification performance of Bayesian Networks Classifiers: a case study of detecting Denial of Service (DoS) attacks in cloud computing environments
- Authors: Moyo, Lindani
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11405 , http://hdl.handle.net/10353/d1021327
- Description: In this research we propose a Bayesian networks approach as a promissory classification technique for detecting malicious traffic due to Denial of Service (DoS) attacks. Bayesian networks have been applied in numerous fields fraught with uncertainty and they have been proved to be successful. They have excelled tremendously in classification tasks i.e. text analysis, medical diagnoses and environmental modeling and management. The detection of DoS attacks has received tremendous attention in the field of network security. DoS attacks have proved to be detrimental and are the bane of cloud computing environments. Large business enterprises have been/or are still unwilling to outsource their businesses to the cloud due to the intrusive tendencies that the cloud platforms are prone too. To make use of Bayesian networks it is imperative to understand the ―ecosystem‖ of factors that are external to modeling the Bayesian algorithm itself. Understanding these factors have proven to result in comparable improvement in classification performance beyond the augmentation of the existing algorithms. Literature provides discussions pertaining to the factors that impact the classification capability, however it was noticed that the effects of the factors are not universal, they tend to be unique for each domain problem. This study investigates the effects of modeling parameters on the classification performance of Bayesian network classifiers in detecting DoS attacks in cloud platforms. We analyzed how structural complexity, training sample size, the choice of discretization method and lastly the score function both individually and collectively impact the performance of classifying between normal and DoS attacks on the cloud. To study the aforementioned factors, we conducted a series of experiments in detecting live DoS attacks launched against a deployed cloud and thereafter examined the classification performance in terms of accuracy of different classes of Bayesian networks. NSL-KDD dataset was used as our training set. We used ownCloud software to deploy our cloud platform. To launch DoS attacks, we used hping3 hacker friendly utility. A live packet capture was used as our test set. WEKA version 3.7.12 was used for our experiments. Our results show that the progression in model complexity improves the classification performance. This is attributed to the increase in the number of attribute correlations. Also the size of the training sample size proved to improve classification ability. Our findings noted that the choice of discretization algorithm does matter in the quest for optimal classification performance. Furthermore, our results indicate that the choice of scoring function does not affect the classification performance of Bayesian networks. Conclusions drawn from this research are prescriptive particularly for a novice machine learning researcher with valuable recommendations that ensure optimal classification performance of Bayesian networks classifiers.
- Full Text:
- Date Issued: 2015
- Authors: Moyo, Lindani
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11405 , http://hdl.handle.net/10353/d1021327
- Description: In this research we propose a Bayesian networks approach as a promissory classification technique for detecting malicious traffic due to Denial of Service (DoS) attacks. Bayesian networks have been applied in numerous fields fraught with uncertainty and they have been proved to be successful. They have excelled tremendously in classification tasks i.e. text analysis, medical diagnoses and environmental modeling and management. The detection of DoS attacks has received tremendous attention in the field of network security. DoS attacks have proved to be detrimental and are the bane of cloud computing environments. Large business enterprises have been/or are still unwilling to outsource their businesses to the cloud due to the intrusive tendencies that the cloud platforms are prone too. To make use of Bayesian networks it is imperative to understand the ―ecosystem‖ of factors that are external to modeling the Bayesian algorithm itself. Understanding these factors have proven to result in comparable improvement in classification performance beyond the augmentation of the existing algorithms. Literature provides discussions pertaining to the factors that impact the classification capability, however it was noticed that the effects of the factors are not universal, they tend to be unique for each domain problem. This study investigates the effects of modeling parameters on the classification performance of Bayesian network classifiers in detecting DoS attacks in cloud platforms. We analyzed how structural complexity, training sample size, the choice of discretization method and lastly the score function both individually and collectively impact the performance of classifying between normal and DoS attacks on the cloud. To study the aforementioned factors, we conducted a series of experiments in detecting live DoS attacks launched against a deployed cloud and thereafter examined the classification performance in terms of accuracy of different classes of Bayesian networks. NSL-KDD dataset was used as our training set. We used ownCloud software to deploy our cloud platform. To launch DoS attacks, we used hping3 hacker friendly utility. A live packet capture was used as our test set. WEKA version 3.7.12 was used for our experiments. Our results show that the progression in model complexity improves the classification performance. This is attributed to the increase in the number of attribute correlations. Also the size of the training sample size proved to improve classification ability. Our findings noted that the choice of discretization algorithm does matter in the quest for optimal classification performance. Furthermore, our results indicate that the choice of scoring function does not affect the classification performance of Bayesian networks. Conclusions drawn from this research are prescriptive particularly for a novice machine learning researcher with valuable recommendations that ensure optimal classification performance of Bayesian networks classifiers.
- Full Text:
- Date Issued: 2015
The effect of grain and soya bean-based diets on chicken production, some egg quality traits, and the potential for allergen carryover to eggs and meat
- Authors: Dokora, Adia-En-Michelle
- Date: 2015
- Subjects: Chickens -- Nutrition Chickens --Feeding and feeds Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2946 , vital:28192
- Description: This study determined the effect of maize-, whole wheat-, soya bean-based-diets on the growth performance, egg quality and dressing percentage of chickens, as well as the potential for soy and gluten allergen carryover to eggs and meat from chickens. Twenty 36-week-old Lorham White (LW) hens were divided into two groups and kept in individual cages until they reached 39-weeks of age, with water and feed supplied ad libitum. Ten LW hens were fed a maize/soya bean-based diet (T1) and the other ten birds were fed a maize/soya bean-based diet with a 15 percent whole wheat inclusion (T2) for a period of four weeks, with weekly individual hen weights and group feed consumption figures being recorded. After a two-week diet adaptation period, six eggs per treatment group were collected every second day from the LW hens’ to measure egg quality traits. Every third day, over a 29-day period, six eggs per treatment were collected and analysed for the presence of soy, gluten and gluten-derived peptides using allergen specific enzyme linked immunosorbent assays (ELISA) kits. For the broiler trial, a total of 160 Ross 308 mixed sex day old chicks were used in a completely randomized design. Two dietary treatments were assigned to eight cages (replicate) per treatment, with ten birds per cage. The Ross 308 broiler feeding programme consisted of three phases, starter (day 1 to day 10), grower (day 11 to day 20) and finisher (day 21 to day 28). At the beginning of the trial all chicks were fed T1 which contained a maize/soya bean-based diet for a period of 10 days and then eight cages with 10 birds per cage were randomly selected and fed the T2 diet, which contained a maize/soya bean-based diet with a 15 percent whole wheat inclusion, until they reached 28 days of age. Weekly live weights, feed intake, average daily gain and the average daily feed intake were recorded and calculated. At the end of the grower (day 21) and finisher phase (day 28), one broiler bird was selected per cage to have eight (8) birds per treatment and 16 birds per phase that were slaughtered and breast meat samples were analysed in duplicate for the presence of soy and gluten allergens using ELISA kits. The dressing percentage of hot carcasses was also determined at the end of the finisher phase on eight birds per treatment. For LW hens, live weight (LW) at 39 weeks of age, the average daily feed intake (ADFI) and weekly feed intakes (FI) were significantly different (P < 0.05) between treatments with birds on T2 recording higher gains. As birds age in weeks increased, significant differences (P < 0.05) in live weight values were recorded. Egg quality traits measured (shell weight, yolk weight, albumen weight, albumen height and Haugh units) were significantly different (P < 0.05) between treatments, with eggs from hens receiving T1 recording higher mean values. As hens got older, the colour of egg yolk improved, producing eggs with higher b* (yellowness) values and lower L* (lightness) and a* (redness) mean values. The Haugh unit, significantly improved (P < 0.05) as the hens got older, signifying better quality of eggs. For the broiler birds, growth traits measured indicated that there were no significant differences (P > 0.05) in live weight (LW), average daily feed intake (ADFI), weekly feed intake (FI), feed conversion ratio (FCR), cumulative feed intake (CFI) and cumulative gain (CG) between treatments. No significant differences (P > 0.05) between dressing percentage of broilers from the two treatments were observed. As birds grew, significant differences (P < 0.05) in LW, FI, AFI, FCR, and CFI, over a four-week period were recorded. Results of the allergen analyses indicated that soy and gluten allergens were not carried over from feed into eggs and meat of chickens fed a maize/soya bean-based diet and a maize/soya bean-based with a 15 percent whole wheat inclusion, respectively. In conclusion, a maize/soya bean-based diet and maize/soya bean-based diet with a 15 percent whole wheat inclusion that contained soy and gluten allergens, are feed sources that promote and stimulate 36-week-old LW hen and Ross 308 chicken growth.
- Full Text:
- Date Issued: 2015
- Authors: Dokora, Adia-En-Michelle
- Date: 2015
- Subjects: Chickens -- Nutrition Chickens --Feeding and feeds Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2946 , vital:28192
- Description: This study determined the effect of maize-, whole wheat-, soya bean-based-diets on the growth performance, egg quality and dressing percentage of chickens, as well as the potential for soy and gluten allergen carryover to eggs and meat from chickens. Twenty 36-week-old Lorham White (LW) hens were divided into two groups and kept in individual cages until they reached 39-weeks of age, with water and feed supplied ad libitum. Ten LW hens were fed a maize/soya bean-based diet (T1) and the other ten birds were fed a maize/soya bean-based diet with a 15 percent whole wheat inclusion (T2) for a period of four weeks, with weekly individual hen weights and group feed consumption figures being recorded. After a two-week diet adaptation period, six eggs per treatment group were collected every second day from the LW hens’ to measure egg quality traits. Every third day, over a 29-day period, six eggs per treatment were collected and analysed for the presence of soy, gluten and gluten-derived peptides using allergen specific enzyme linked immunosorbent assays (ELISA) kits. For the broiler trial, a total of 160 Ross 308 mixed sex day old chicks were used in a completely randomized design. Two dietary treatments were assigned to eight cages (replicate) per treatment, with ten birds per cage. The Ross 308 broiler feeding programme consisted of three phases, starter (day 1 to day 10), grower (day 11 to day 20) and finisher (day 21 to day 28). At the beginning of the trial all chicks were fed T1 which contained a maize/soya bean-based diet for a period of 10 days and then eight cages with 10 birds per cage were randomly selected and fed the T2 diet, which contained a maize/soya bean-based diet with a 15 percent whole wheat inclusion, until they reached 28 days of age. Weekly live weights, feed intake, average daily gain and the average daily feed intake were recorded and calculated. At the end of the grower (day 21) and finisher phase (day 28), one broiler bird was selected per cage to have eight (8) birds per treatment and 16 birds per phase that were slaughtered and breast meat samples were analysed in duplicate for the presence of soy and gluten allergens using ELISA kits. The dressing percentage of hot carcasses was also determined at the end of the finisher phase on eight birds per treatment. For LW hens, live weight (LW) at 39 weeks of age, the average daily feed intake (ADFI) and weekly feed intakes (FI) were significantly different (P < 0.05) between treatments with birds on T2 recording higher gains. As birds age in weeks increased, significant differences (P < 0.05) in live weight values were recorded. Egg quality traits measured (shell weight, yolk weight, albumen weight, albumen height and Haugh units) were significantly different (P < 0.05) between treatments, with eggs from hens receiving T1 recording higher mean values. As hens got older, the colour of egg yolk improved, producing eggs with higher b* (yellowness) values and lower L* (lightness) and a* (redness) mean values. The Haugh unit, significantly improved (P < 0.05) as the hens got older, signifying better quality of eggs. For the broiler birds, growth traits measured indicated that there were no significant differences (P > 0.05) in live weight (LW), average daily feed intake (ADFI), weekly feed intake (FI), feed conversion ratio (FCR), cumulative feed intake (CFI) and cumulative gain (CG) between treatments. No significant differences (P > 0.05) between dressing percentage of broilers from the two treatments were observed. As birds grew, significant differences (P < 0.05) in LW, FI, AFI, FCR, and CFI, over a four-week period were recorded. Results of the allergen analyses indicated that soy and gluten allergens were not carried over from feed into eggs and meat of chickens fed a maize/soya bean-based diet and a maize/soya bean-based with a 15 percent whole wheat inclusion, respectively. In conclusion, a maize/soya bean-based diet and maize/soya bean-based diet with a 15 percent whole wheat inclusion that contained soy and gluten allergens, are feed sources that promote and stimulate 36-week-old LW hen and Ross 308 chicken growth.
- Full Text:
- Date Issued: 2015
The implications of smallholder agricultural productivity growth for poverty alleviation in post-apartheid South Africa
- Authors: Dwesini, Nontembiso Eugenia
- Date: 2015
- Subjects: Agriculture -- Economic aspects -- South Africa , Agricultural assistance -- South Africa , Sustainable development -- South Africa , Agricultural productivity -- South Africa
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11232 , http://hdl.handle.net/10353/d1021286 , Agriculture -- Economic aspects -- South Africa , Agricultural assistance -- South Africa , Sustainable development -- South Africa , Agricultural productivity -- South Africa
- Description: The adoption of the Millennium Development Goal 1(MDG 1) of reducing the rate of poverty to half of the 1990-level by 2015 has been a challenge faced by the developing countries including South Africa. The foundations of democracy have to be continuously assessed so that the obstacles faced by South Africa as it strives towards sustainable democracy are addressed. With agricultural sector identified as having the potential to alleviate poverty compared to the mining sector, manufacturing sector and services sector, it faced the challenges that include: (i) accelerating agricultural productivity; (ii) reducing poverty and vulnerability; and (iii) narrowing rural-urban income disparities. The identification of the critical linkages in the agricultural development framework allows for effective strategic planning, effective decision making and appropriate policy formulation. Expectedly, the sector has attracted considerable fiscal policy interest and public investments. The primary aim of this research study is to assess the extent to which smallholder agricultural productivity growth alleviates poverty in South Africa. The statistical and econometric techniques namely; Johansen technique of co-integration analysis (1995), analysis of covariance and correlation, Vector Error Correction Model, are employed in this research study. The data description, data sources, expected relationship between variables and indexation of data are done. The drivers and cause-effect relationships between agriculture and poverty reduction are investigated. The employed models allowed for an exploration of plausible future growth in agricultural elasticity of poverty and the possibility of reducing poverty level in South Africa. The data is obtained from the National Department of Agriculture from 1994 -2013. The analysis of the results strongly confirms that agricultural productivity has a significant inverse relationship to the levels of poverty in South Africa. The outcome of the analysis will contribute to improved decision making on the use of public funds in agriculture.
- Full Text:
- Date Issued: 2015
- Authors: Dwesini, Nontembiso Eugenia
- Date: 2015
- Subjects: Agriculture -- Economic aspects -- South Africa , Agricultural assistance -- South Africa , Sustainable development -- South Africa , Agricultural productivity -- South Africa
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11232 , http://hdl.handle.net/10353/d1021286 , Agriculture -- Economic aspects -- South Africa , Agricultural assistance -- South Africa , Sustainable development -- South Africa , Agricultural productivity -- South Africa
- Description: The adoption of the Millennium Development Goal 1(MDG 1) of reducing the rate of poverty to half of the 1990-level by 2015 has been a challenge faced by the developing countries including South Africa. The foundations of democracy have to be continuously assessed so that the obstacles faced by South Africa as it strives towards sustainable democracy are addressed. With agricultural sector identified as having the potential to alleviate poverty compared to the mining sector, manufacturing sector and services sector, it faced the challenges that include: (i) accelerating agricultural productivity; (ii) reducing poverty and vulnerability; and (iii) narrowing rural-urban income disparities. The identification of the critical linkages in the agricultural development framework allows for effective strategic planning, effective decision making and appropriate policy formulation. Expectedly, the sector has attracted considerable fiscal policy interest and public investments. The primary aim of this research study is to assess the extent to which smallholder agricultural productivity growth alleviates poverty in South Africa. The statistical and econometric techniques namely; Johansen technique of co-integration analysis (1995), analysis of covariance and correlation, Vector Error Correction Model, are employed in this research study. The data description, data sources, expected relationship between variables and indexation of data are done. The drivers and cause-effect relationships between agriculture and poverty reduction are investigated. The employed models allowed for an exploration of plausible future growth in agricultural elasticity of poverty and the possibility of reducing poverty level in South Africa. The data is obtained from the National Department of Agriculture from 1994 -2013. The analysis of the results strongly confirms that agricultural productivity has a significant inverse relationship to the levels of poverty in South Africa. The outcome of the analysis will contribute to improved decision making on the use of public funds in agriculture.
- Full Text:
- Date Issued: 2015
The investigation of perceptions of professional nurses regarding care of mental health care users in a general hospital setting
- Manona-Nkanjeni, Nonkanyiso Yvonne
- Authors: Manona-Nkanjeni, Nonkanyiso Yvonne
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11924 , http://hdl.handle.net/10353/d1020200
- Description: This study sought to explore the perceptions of professional nurses regarding care of stabilised mental health care users in a general hospital setting. A qualitative, explanatory, descriptive and contextual design was used for the study. A non-probability, purposive sampling method was used to select 12 participants from the Cecilia Makiwane Hospital in Mdantsane. Data were collected through semi-structured interviews. The services of an independent interviewer were used to avoid any bias as interviews took place where the researcher is employed. The services of an editor were also used for language control (see Annexure H). The researcher repeatedly listened to the tapes used for data collection until completely satisfied with the interpretation of verbatim data. The research study was conducted in an ethically reflective manner and trustworthiness was ensured at all times. Four themes emerged from the analysis of the interviews: fear, stigma, myths and training. The researcher utilised the services of an independent coder who verified the identified major themes. The findings revealed that participants were fearful due to lack of knowledge, experience and psychiatric nursing skills. Participants feared because they lack knowledge about psychiatric medication and because mental health care users may have relapse. The participants also attached a stigma to mental health care users, which resulted in poor communication between participants and the stabilised mental health care user; a negative attitude towards mental health care users; and non-acceptance. Participants believed in myths about mental illness; they regarded it as contagious; and perceived mental health care users as dangerous. The participants strongly recommended that training should be provided to improve their knowledge and skills to enable them to care for stabilised mental health care users in a general hospital setting. The following should be facilitated: in-service training; adoption of a positive attitude; dispersal of myths and fear; education about referral systems; and allocation of specialist psychiatric nurses to medical wards for referral purposes with regard to complicated cases.
- Full Text:
- Date Issued: 2015
- Authors: Manona-Nkanjeni, Nonkanyiso Yvonne
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11924 , http://hdl.handle.net/10353/d1020200
- Description: This study sought to explore the perceptions of professional nurses regarding care of stabilised mental health care users in a general hospital setting. A qualitative, explanatory, descriptive and contextual design was used for the study. A non-probability, purposive sampling method was used to select 12 participants from the Cecilia Makiwane Hospital in Mdantsane. Data were collected through semi-structured interviews. The services of an independent interviewer were used to avoid any bias as interviews took place where the researcher is employed. The services of an editor were also used for language control (see Annexure H). The researcher repeatedly listened to the tapes used for data collection until completely satisfied with the interpretation of verbatim data. The research study was conducted in an ethically reflective manner and trustworthiness was ensured at all times. Four themes emerged from the analysis of the interviews: fear, stigma, myths and training. The researcher utilised the services of an independent coder who verified the identified major themes. The findings revealed that participants were fearful due to lack of knowledge, experience and psychiatric nursing skills. Participants feared because they lack knowledge about psychiatric medication and because mental health care users may have relapse. The participants also attached a stigma to mental health care users, which resulted in poor communication between participants and the stabilised mental health care user; a negative attitude towards mental health care users; and non-acceptance. Participants believed in myths about mental illness; they regarded it as contagious; and perceived mental health care users as dangerous. The participants strongly recommended that training should be provided to improve their knowledge and skills to enable them to care for stabilised mental health care users in a general hospital setting. The following should be facilitated: in-service training; adoption of a positive attitude; dispersal of myths and fear; education about referral systems; and allocation of specialist psychiatric nurses to medical wards for referral purposes with regard to complicated cases.
- Full Text:
- Date Issued: 2015
Tillage effects on the aggregate-associated organic carbon and bulk density in some South African soils with different texture
- Authors: Njeru, Sarah Kangai
- Date: 2015
- Subjects: Soil management Tillage
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2512 , vital:27881
- Description: Tillage operations disrupt the soil structure resulting in aggregates of various sizes and altered bulk density. Moreover, tillage influences soil carbon pools and many other soil physical properties. The objectives of this study were to determine, in various South African soils under different tillage systems, the following. (1) Amount of aggregate-associated soil organic carbon (SOC), (2) soil compressibility, and (3) relationship between compressibility, texture and the aggregate-associated SOC. The soil samples used in this study were collected from six different sites in Eastern Cape Province, South Africa. Soil samples were taken from conventional tillage (CT) and no-till (NT) land. To keep the soil aggregates intact sampling was done using a spade and carefully carried to the laboratory in rigid containers. For SOC determination, treatments were the two tillage systems, CT and NT, and four aggregate sizes. The experimental design was completely randomized design with a factorial layout and was replicated three times. Aggregate-associated SOC was determined using Walkley-Black method. Proctor compaction test was used determine the dry bulk density with varying moisture content and consequently the maximum bulk density (MBD) and critical water content (CWC). The aggregate-associated SOC content differed with tillage system and was significant higher (p < 0.05) in CT than NT. The amount of aggregate-associated SOC was 1.67 times higher in CT than NT plots. The MBD ranged between 1.77 g/cm3 and 10.27 g/cm3 and the CWC ranged from 9.1 percent to 10.3 percent. The higher amounts of SOC in CT were attributed to the annual crop residue returns while the lower amounts of SOC in the NT fields were due to grazing. Therefore, tillage influenced the amount of aggregate-associated organic carbon irrespective of the resulting size of the aggregate. The positive relationship between tillage and aggregate-associated SOC challenges the conversion of land to no-till for carbon sequestration. The overall gradient for correlation between the MBD and CWC was negative with r2 = 0.23 and a p value of 0.0076. The compressibility curves indicated higher values under CT if the texture class was silt clay.
- Full Text:
- Date Issued: 2015
- Authors: Njeru, Sarah Kangai
- Date: 2015
- Subjects: Soil management Tillage
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2512 , vital:27881
- Description: Tillage operations disrupt the soil structure resulting in aggregates of various sizes and altered bulk density. Moreover, tillage influences soil carbon pools and many other soil physical properties. The objectives of this study were to determine, in various South African soils under different tillage systems, the following. (1) Amount of aggregate-associated soil organic carbon (SOC), (2) soil compressibility, and (3) relationship between compressibility, texture and the aggregate-associated SOC. The soil samples used in this study were collected from six different sites in Eastern Cape Province, South Africa. Soil samples were taken from conventional tillage (CT) and no-till (NT) land. To keep the soil aggregates intact sampling was done using a spade and carefully carried to the laboratory in rigid containers. For SOC determination, treatments were the two tillage systems, CT and NT, and four aggregate sizes. The experimental design was completely randomized design with a factorial layout and was replicated three times. Aggregate-associated SOC was determined using Walkley-Black method. Proctor compaction test was used determine the dry bulk density with varying moisture content and consequently the maximum bulk density (MBD) and critical water content (CWC). The aggregate-associated SOC content differed with tillage system and was significant higher (p < 0.05) in CT than NT. The amount of aggregate-associated SOC was 1.67 times higher in CT than NT plots. The MBD ranged between 1.77 g/cm3 and 10.27 g/cm3 and the CWC ranged from 9.1 percent to 10.3 percent. The higher amounts of SOC in CT were attributed to the annual crop residue returns while the lower amounts of SOC in the NT fields were due to grazing. Therefore, tillage influenced the amount of aggregate-associated organic carbon irrespective of the resulting size of the aggregate. The positive relationship between tillage and aggregate-associated SOC challenges the conversion of land to no-till for carbon sequestration. The overall gradient for correlation between the MBD and CWC was negative with r2 = 0.23 and a p value of 0.0076. The compressibility curves indicated higher values under CT if the texture class was silt clay.
- Full Text:
- Date Issued: 2015