- Title
- Metabarcoding Analysis of Bacterial Communities Associated with Media Grow Bed Zones in an Aquaponic System
- Creator
- Kasozi, Nasser
- Creator
- Kaiser, Horst
- Creator
- Wilhelmi, Brendan S
- Subject
- To be catalogued
- Date Issued
- 2020
- Date
- 2020
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/429461
- Identifier
- vital:72612
- Identifier
- xlink:href="https://doi.org/10.1155/2020/8884070"
- Description
- The development of environmentally sustainable plant and fish production in aquaponic systems requires a complete understanding of the systems’ biological components. In order to better understand the role of microorganisms in this association, we studied the bacterial communities in the dry, root, and mineralized zones of a flood-and-drain media bed aquaponic system. Bacterial communities were characterized using metabarcoding of the V3-V4 16S rRNA regions obtained from paired-end Illumina MiSeq reads. Proteobacteria, Actinobacteria, and Bacteroidetes accounted for more than 90% of the total community in the dry zone and the effluent water. These phyla also accounted for more than 68% of the total community in the root and mineralized zones. The genera Massilia, Mucilaginibacter, Mizugakiibacter, and Rhodoluna were most dominant in the dry, root, and mineralized zones and in the effluent water, respectively. The number of shared operational taxonomic units (OTUs) for the three zones was 241, representing 7.15% of the total observed OTUs. The number of unique OTUs in samples from dry zone, root zone, mineralized zone, and effluent water was 485, 638, 445, and 383, respectively. The samples from the root zone harbored more diverse communities than either the dry or mineralized zones. This study is the first to report on the bacterial community within the zones of a flood-and-drain media bed. Thus, this information will potentially accelerate studies on other microbial communities involved in the bioconversion of nitrogen compounds and mineralization within these types of aquaponic systems.
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (10 pages)
- Format
- Publisher
- Hindawi
- Language
- English
- Relation
- International Journal of Microbiology
- Relation
- Kasozi, N., Kaiser, H. and Wilhelmi, B. (2020) Metabarcoding Analysis of Bacterial Communities Associated with Media Grow Bed Zones in an Aquaponic System. International Journal of Microbiology. 2020 (8884070). p.1-10
- Relation
- International Journal of Microbiology volume 8884070 p. 1 2020 1687-9198
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Hindawi Copyright Statement (https://www.hindawi.com/copyright/)
- Rights
- Closed Access
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Collections
Wilhelmi, Brendan (Dr)
Kaiser, Horst (Prof)
RU Department of Ichthyology and Fisheries Science
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 14. Life Below Water
SDG 15. Life On Land
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