Electrodeposited Benzothiazole Phthalocyanines for Corrosion Inhibition of Aluminium in Acidic Medium
- Authors: Nnaji, Nnaemeka , Nwaji, Njemuwa , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186112 , vital:44464 , xlink:href="https://doi.org/10.1155/2020/8892559"
- Description: Tetrakis[(benzo[d]thiazol-2-yl-thio) phthalocyaninato] gallium(III)chloride (1) and tetrakis[(benzo[d]thiazol-2ylphenoxy) phthalocyaninato] gallium(III)chloride (2) were successfully electrodeposited onto aluminium for corrosion retardation in 1.0 M hydrochloric acid solution. The aim of this study was to compare the corrosion resistance of electrodeposited metallated phthalocyanines. Scanning electron microscopy, X-ray diffraction, electrochemical impedance spectroscopy (EIS), and polarization confirmed the aluminium corrosion inhibition potentials of complexes 1 and 2. EIS and polarization techniques showed that complex 2 performed better than complex 1, with values from EIS measurements of 82% for 1 and 86% for 2 in 1.0 M hydrochloric acid solution. The importance of electrodeposition in industries and a dearth of research on the use of electrodeposited metallated phthalocyanines necessitated this study, and results show that coatings formed by electrodeposition of 1 and 2 onto aluminium reduced its susceptibility to corrosion attack.
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- Date Issued: 2020
Metabarcoding Analysis of Bacterial Communities Associated with Media Grow Bed Zones in an Aquaponic System
- Authors: Kasozi, Nasser , Kaiser, Horst , Wilhelmi, Brendan
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429461 , vital:72612 , xlink:href="https://doi.org/10.1155/2020/8884070"
- Description: The development of environmentally sustainable plant and fish production in aquaponic systems requires a complete understanding of the systems’ biological components. In order to better understand the role of microorganisms in this association, we studied the bacterial communities in the dry, root, and mineralized zones of a flood-and-drain media bed aquaponic system. Bacterial communities were characterized using metabarcoding of the V3-V4 16S rRNA regions obtained from paired-end Illumina MiSeq reads. Proteobacteria, Actinobacteria, and Bacteroidetes accounted for more than 90% of the total community in the dry zone and the effluent water. These phyla also accounted for more than 68% of the total community in the root and mineralized zones. The genera Massilia, Mucilaginibacter, Mizugakiibacter, and Rhodoluna were most dominant in the dry, root, and mineralized zones and in the effluent water, respectively. The number of shared operational taxonomic units (OTUs) for the three zones was 241, representing 7.15% of the total observed OTUs. The number of unique OTUs in samples from dry zone, root zone, mineralized zone, and effluent water was 485, 638, 445, and 383, respectively. The samples from the root zone harbored more diverse communities than either the dry or mineralized zones. This study is the first to report on the bacterial community within the zones of a flood-and-drain media bed. Thus, this information will potentially accelerate studies on other microbial communities involved in the bioconversion of nitrogen compounds and mineralization within these types of aquaponic systems.
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- Date Issued: 2020
Investigating the chaperone properties of a novel heat shock protein, Hsp70. c, from Trypanosoma brucei
- Authors: Burger, Adélle , Ludewig, Michael H , Boshoff, Aileen
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431155 , vital:72749 , xlink:href="https://doi.org/10.1155/2014/172582"
- Description: The neglected tropical disease, African Trypanosomiasis, is fatal and has a crippling impact on economic development. Heat shock protein 70 (Hsp70) is an important molecular chaperone that is expressed in response to stress and Hsp40 acts as its co-chaperone. These proteins play a wide range of roles in the cell and they are required to assist the parasite as it moves from a cold blooded insect vector to a warm blooded mammalian host. A novel cytosolic Hsp70, from Trypanosoma brucei, TbHsp70.c, contains an acidic substrate binding domain and lacks the C-terminal EEVD motif.The ability of a cytosolic Hsp40 from Trypanosoma brucei J protein 2, Tbj2, to function as a co-chaperone of TbHsp70.c was investigated.The main objective was to functionally characterize TbHsp70.c to further expand our knowledge of parasite biology. TbHsp70.c and Tbj2 were heterologously expressed and purified and both proteins displayed the ability to suppress aggregation of thermolabile MDH and chemically denatured rhodanese. ATPase assays revealed a 2.8-fold stimulation of the ATPase activity of TbHsp70.c by Tbj2. TbHsp70.c and Tbj2 both demonstrated chaperone activity and Tbj2 functions as a co-chaperone of TbHsp70.c.In vivo heat stress experiments indicated upregulation of the expression levels of TbHsp70.c.
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- Date Issued: 2014