A framework for designing a gamified system that promotes knowledge of water sustainability
- Authors: Kunjuzwa, Dumani Tau
- Date: 2024-12
- Subjects: Computer science , User interfaces (Computer systems) , Water-supply -- Management
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69349 , vital:77216
- Description: Water shortages are becoming increasingly common and raising awareness thereof has been proposed as a key strategy for empowering citizens with knowledge relevant to promoting water sustainability. This knowledge should incorporate indigenous knowledge, which is unique and confined to a particular culture. Indigenous knowledge has been recognised as the key to sustainable social and economic development. Knowledge incorporated within appropriate technologies can be a transformative solution to promote sustainable consumption of natural resources. This study aimed to develop a framework with strong theoretical connections that can be used as a guide for designing and developing technological solutions for promoting knowledge, attitude, and practices (KAP) of water sustainability. Gamification, serious games, and community social networks were identified as appropriate technologies for implementing these solutions. Design Science Research (DSR) was an appropriate research methodology for this research. As the requirement of the DSR methodology, a framework was designed that considered relevant theories. The two theories (Self-Determination Theory and Social Capital Theory) were incorporated into the framework. The framework can guide other researchers in developing gamified systems that promote the KAPs of water sustainability. This framework was the theoretical artefact of the study. The research context was the Eastern Cape Province of South Africa where the data was collected from two municipalities namely, the Nelson Mandela Bay and Buffalo City Metro, which have both experienced water shortages in recent years. Quantitative and qualitative data collection methods were used. Qualitative data was collected from brainstorming sessions and expert reviews that were conducted with various stakeholders that assisted with designing and evaluating the prototype designs of the gamified solution. For the design of the study’s artefacts, a participatory research approach was adopted. Participants were recruited to complete an online survey, which ran for three months. The findings revealed that citizens’ knowledge is closely correlated to the attitudes and practices of citizens regarding water sustainability. In addition, the findings showed that the knowledge of the citizens in the Eastern Cape Province was relatively low on water practices such as conservation and filtering methods. These results were considered in the design of the practical artefact and the instantiation findings. One limitation of this study was the limited number of indigenous participants in the survey. Other limitations were the lack of experts with indigenous knowledge and the lack of indigenous knowledge documentation. The promotion of water sustainability knowledge in communities would be more effective with the use of gamification and social community networks. In principle, the enhancement in water sustainability knowledge would promote the citizens’ good practices and attitudes (behavioural) related to water sustainability. Since 2015, a significant portion of South Africa's Eastern Cape Province has been suffering from a severe drought. This province is one of the regions of the country where weather patterns have changed dramatically, and as a result, most households were left vulnerable to the water shortage problem. While the broad topic of water sustainability is widely discussed in the literature, the role of appropriate technologies in promoting knowledge of water sustainability is still under-researched. This gap provided a significant potential for investigation by sustainability to design a technological and theoretical solution for promoting knowledge of water sustainability. This study contributes to the body of knowledge by delivering three DSR artefacts namely, a Framework for Designing a Gamified System that Promotes Knowledge of Water Sustainability; the design guidelines that supplement the framework, and a practical instantiation of the framework. , Thesis (DPhil) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2024
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- Date Issued: 2024-12
A model to facilitate nursing research supervisors in assisting postgraduate nursing students in Gauteng Province with timeous completion of studies
- Authors: Mbombi, Masenyani Oupa
- Date: 2024-12
- Subjects: College dropouts -- Gauteng -- South Africa , Nursing -- Study and teaching -- Gauteng -- South Africa , Nursing -- Research
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69727 , vital:78014
- Description: Many academically orientated persons aspire towards having postgraduate qualifications for various reasons. The reasons range from career development to expanding their knowledge in fields related to their current areas of professional specialisation. Some people however do not fulfil that personal need due to academic exclusion from their studies in the university related to taking too long to finish their studies while others just drop out. The current study aimed to develop a model that would assist in facilitating the supervision of postgraduate nursing students to complete their studies in the required and allocated time at nursing education institutions in Gauteng. The objectives of the study were to: explore and describe the perceptions of postgraduate research nursingstudents regarding their preparedness for a joint research journey; explore and describe the perceptions of research supervisors regarding theirpreparedness for a joint research journey; develop a conceptual framework that would underpin the development of amodel to facilitate the preparedness of postgraduate research nursing students(PGRNSs) and supervisors for the research journey at NEIs in Gauteng; and develop and describe a model to facilitate the preparedness of postgraduateresearch nursing students (PGRNSs) and inexperienced research supervisorsfor a joint research journey at NEIs at Gauteng. A theory-generative research design based on a qualitative, explorative, descriptive, and contextual research approach was adopted in this study. Purposive sampling was used to select 16 postgraduate nursing students (8 master’s and 8 doctoral) and 12 research supervisors from universities in Gauteng, South Africa. Data collection was conducted through voice-recorded unstructured interviews, which were transcribed verbatim and analysed using Tesch’s open coding method of data analysis. To ensure the trustworthiness of the study, the researcher used various strategies based on the criteria recommended in Guba’s model of trustworthiness. The criteria were credibility, transferability, confirmability and dependability. Ethical principles, such as respect for a person’s beneficence, non-maleficence, and justice were ensured during the data collection stage. Four broad themes (see Chapter 3) emerged from the data analysis. The themes were: Theme: perceptions regarding postgraduate supervision provided or received Theme: perceptions regarding preparedness for the research journey Theme: support systems influencing preparedness for the research journey Theme: Hope for change in the research progress and concerns to be considered Concept identification and analysis assisted the researcher in putting concepts in a relationship for the development of the model as guided by the six survey elements of Dickoff and James. Although the model was not tested, the researcher and the selected nursing experts were confident that the model is significant to nursing and other disciplines. The model was described and successfully evaluated by a panel of six nursing experts who acknowledged the clarity, generality, accessibility, simplicity, and significant contribution to the nursing environment. The guidelines and recommendations to implement the model in the nursing environment were developed to enhance the application of the model. The model aims to provide a theoretical and structural frame of reference to the head of the department and research supervisors in nursing for the facilitation of the preparedness of the postgraduate students and inexperienced supervisors for a joint research journey to ensure the timeous completion of postgraduate studies. The outcomes of the model include easing the struggle of students during their research studies for acceptable progress within the nursing environment of nursing education institutions. inexperienced research supervisors who experienced challenges to achieving optimum supervision in the nursing environment will also benefit if they apply the developed model. To enhance the applicability of the model, the researcher recommends that the model be tested in postgraduate studies for future benefits before implementation. , Thesis (PhD) -- Faculty of Health Sciences, School of Clinical Care & Medicinal Sciences, 2024
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- Date Issued: 2024-12
A multistep synthesis of zidovudine in continuous flow systems
- Authors: Kwakhanya, Mkwakwi
- Date: 2024-12
- Subjects: HIV -- South Africa , HIV infections -- Diagnosis , Antiretroviral agents -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69393 , vital:77251
- Description: Universal access to antiretroviral drugs is still a challenge in developing nations. This promoted an investigation into an efficient process towards the synthesis of zidovudine (AZT), a nucleoside reverse transcriptase inhibitor (NRTI) that is used to treat HIV/AIDS. It was the first antiretroviral drug approved by the FDA and it is still widely used today. AZT works by blocking the enzyme reverse transcriptase, which is used by HIV to copy its genetic material. This prevents HIV from replicating and spreading throughout the body. AZT is usually taken in combination with other antiretroviral drugs. AZT was originally used as a single-drug therapy, but it is no longer recommended for this purpose as HIV can become resistant to AZT if it is used alone. Consequently, AZT is most commonly used as part of a combination therapy; this means that it is taken with other antiretroviral drugs. This helps to prevent HIV from becoming resistant to AZT. AZT can be used to prevent mother-to-child transmission of HIV; it is usually given to HIV-positive women during pregnancy and childbirth. AZT can also be used to treat post-exposure prophylaxis (PEP); this is a course of antiretroviral drugs that is taken after a possible exposure to HIV. The application of continuous flow microreactors to reaction chemistries, such as this, could provide a potentially practical solution that could be used to meet the rising needs for antiretroviral drugs in Africa and to give new opportunities for efficient local drug manufacture. Consequently, the focus of this research was to develop safe and efficient processes towards AZT by taking advantage of micro reactor technology. Furthermore, since there is no literature on continuous flow synthesis of this drug, this research extensively unearthed interesting continuous flow processes towards zidovudine. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
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- Date Issued: 2024-12
A relevant intervention on nutrition transition among Isixhosa speaking people in Nelson Mandela Bay
- Authors: Fordjour Afriyie, Vera
- Date: 2024-12
- Subjects: Nutrition -- South Africa -- Port Elizabeth , Nutrition -- Social aspects , Xhosa (African people) -- Nutrition
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69760 , vital:78026
- Description: The nutrition transition, characterised by a shift from traditional, nutrient-dense diets to Westernised, energy-dense, and nutrient-poor diets, has significant health implications globally. In South Africa (SA), particularly among the isiXhosa-speaking population in Nelson Mandela Bay (NMB), this transition has led to the dual burden of undernutrition and overnutrition, increasing the prevalence of non-communicable diseases (NCDs) such as obesity and diabetes. Despite the prevalence of this nutrition phenomenon, limited research has explored the perceptions of nutrition transition among South African communities, although there is literature about the transition itself. Most studies on the research subject were undertaken in Asian and European countries and America. Generally, studies regarding the nutrition transition emphasise the importance of early public health interventions to prevent the transition from reaching critical levels. This research study aimed to answer the question: How do isiXhosa-speaking people in NMB perceive nutritional transition, and what are their knowledge and practices concerning traditional foods? This study’s primary aim was to identify and evaluate the NMB isiXhosa-speaking population’s perceptions regarding nutrition transition and their knowledge and practices concerning traditional foods. Subsequently, the study aimed to develop and test an educational intervention to promote traditional African diets and mitigate the adverse effects of nutrition transition. The researcher employed a multi-method approach, encompassing quantitative and qualitative methods and an exploratory, descriptive, and contextual approach to enhance the research design. Ethical principles guided the study, including autonomy, non-maleficence, beneficence, and justice. The study comprised two phases. Participants were selected through a stratified, clustered sampling technique. In the first phase, data were collected through in-depth interviews, focus group discussions (FGDs), a 24-hour dietary recall questionnaire, and a knowledge and practices The participants were isiXhosa-speaking NMB residents older than 18 years of age. The second phase utilised the first-phase findings to develop an intervention programme. The intervention was implemented, and its efficacy was evaluated through post-intervention surveys, sensory evaluation, and a three-month telephonic follow-up. The findings indicated a significant decline in traditional food knowledge (TFK) and practices among the participants, with a marked shift towards Westernised diets. The primary factors driving this transition were urbanisation, economic constraints, and a lack of awareness about the nutritional benefits of traditional foods. The intervention programme, which included educational workshops and a sensory evaluation using indigenous crops, resulted in improved knowledge and practices regarding indigenous crops among the participants. Post-intervention data showed increased dietary diversity and a greater inclusion of traditional foods in daily diets. The study concluded that nutrition transition poses a significant challenge to the health and well-being of isiXhosa-speaking individuals in NMB. However, targeted interventions that promote traditional African diets can effectively address this issue. The study recommends that policymakers and public health practitioners incorporate culturally relevant and community-driven strategies to promote traditional food consumption. Future research should focus on the long-term impacts of such interventions and explore scalable models for broader implementation. , Thesis (PhD) -- Faculty of Health Sciences, School of Behavioural & Lifestyle Sciences, 2024
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- Date Issued: 2024-12
A strategy towards an it governance-focused implementation of Egovernment initiatives in the Eastern Cape provincial government
- Authors: Magade, Siyabonga
- Date: 2024-12
- Subjects: Internet in public administration , Government communication systems , Public administration -- Data processing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68725 , vital:77085
- Description: ICT plays a very important role in transforming organisations as well as governments around the world through eGovernment. A good implementation of eGovernment promises to transform and enhance the provision of services to citizens. The benefits of eGovernment will enable the transparency of government and engender the trust of its citizens therein. Citizens will have access to online government services that will minimise the long queues that citizens need to endure to access government services. eGovernment implementation requires ICT technologies, however, dependency on the implementation of ICT technologies to drive eGovernment initiatives will not alone succeed in transforming the delivery of services in government. Therefore, the implementation of ICT technologies in government must be focused on good IT governance principles. Such principles enable the executive council and management to direct and control the application both now and in the future of use of ICT in government. Therefore, this study develops a strategy artefact that is focused on good IT governance principles to assist in the execution of eGovernment programs in the Eastern Cape Provincial Government. , Thesis (MPhil) -- Faculty of Engineering, the Built Environment, and Technology, School of Information Technology, 2024
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- Date Issued: 2024-12
Achievement in mathematics through self-directed learning facilitation: an impact evaluation of an offline technological approach
- Authors: Zinn, Paméla Olive
- Date: 2024-12
- Subjects: Mathematics -- Study and teaching (Secondary) -- South Africa , Mathematical ability , Academic achievement -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68385 , vital:76992
- Description: International assessments indicate that mathematics education in South Africa is still in a crisis of significant proportions. This is reflected in the low pass rate of Grade 12 mathematics learners in South Africa in general, and in the Eastern Cape Province in particular. In response, the Govan Mbeki Mathematics Development Center (GMMDC) of the Nelson Mandela University (NMU) in the Eastern Cape developed an intervention strategy. A Techno-Blended support Model for teaching and learning mathematics (TBM), used as an intervention strategy, provides learners with potential from disadvantaged communities and under-resourced schools with 21st-century education technology. The defining features of the strategy which distinguishes it from others are that it is technology-enhanced, providing the learner access to mathematics teaching and learning without the need for Internet connectivity, that is, offline, incorporating blended learning into the approach. This ex-post study, using secondary analysis, evaluates the impact of the defining features of the strategy on learners' self-directed learning skills acquisition or enhancement for mathematics learning. Firstly, statistical analysis of secondary qualitative and quantitative data in a mixed methods approach, with primary qualitative data integrated into the secondary qualitative data employing quantification, is undertaken. The quantified data is then compared with the quantitative data (methodologic triangulation technique). Secondly, the theory-based Contribution Analysis is undertaken to increase the validity and credibility of the findings of the statistical analysis, providing an additional layer of triangulation. The study revealed that providing 21st-century digital education technology for use offline, within a blended learning environment, leads to a significant improvement in learner mathematics knowledge; conceptual understanding; positive self-perceptions; and finally, to the required intrinsic motivation to learn. Analysed data, obtained from the original study, indicates a positive correlation between learners who participated in the intervention strategy and enrolment in STEM-related courses at tertiary institutes. , Thesis (DPhil) -- Faculty of Education, School of Postgraduate education, 2024
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- Date Issued: 2024-12
Amagqirha (Xhosa diviners) and the treatment of mental illness: exploring indigenous healing practices and the potential for integrating indigenous knowledge into psychology training
- Authors: Klaas, Fihliwe
- Date: 2024-12
- Subjects: Mental illness -- Treatment , Psychology -- Africa , Healers -- South Africa -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69860 , vital:78042
- Description: This study focuses on Amagqirha kwa Xhosa (diviners) who are traditional healers within the Xhosa community. Despite their impact and footprint in the communities, there is limited knowledge regarding their role in managing mental health illnesses, particularly the kind of techniques they employ. In light of this, the study is aimed at exploring the types of techniques and interventions utilized by Amagqirha kwa Xhosa in treating psychological problems and assessing how the knowledge can be incorporated into the training of psychologists. The study employed a qualitative approach with semi-structured one-on-one interviews, using purposive and snowballing sampling strategies. Eight traditional healers from the Nelson Mandela Bay municipality, Eastern Cape, participated in the study, and thematic data analysis was utilised to analyse the data. Two primary themes were revealed in Amagqirha’s experiences with treating mental illness, and the first set core themes are 1. Ukuxilonga (Diagnosing) and 2. Izinto ngokwa ses’Xhoseni ezenza ingulo ye ngqondo (Xhosa Realities and Causes of Mental Illnesses). The second set of core themes revealed Amagqirha’s treatment methods and interventions, and these are 1.Indlela Zokunyanga Zesintu (Tradition Methods of Healing) and 2.Impawu zoxilongo olunemiphumela zobuGqirha (Treatment efficacy in traditional healing). The findings highlight the importance of recognizing diverse knowledge systems in Mental Health care, emphasizing the need for culturally sensitive approaches that respect and take into account cultural context and spirituality of the community/patients. Additionally, the study provides guidelines for mental health practitioners when dealing with clients who align with paradigms other than the Western biomedical paradigm or worldview, highlighting the importance of integrating varying paradigms in approaching mental health practice. , Thesis (MA) -- Faculty of Health Sciences, School of Behavioural & Lifestyle Sciences, 2024
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- Date Issued: 2024-12
Ammendments to the labour law relations act 66 of 1995 to addressing violent and intractable strikes
- Authors: Gavu, Siphelele
- Date: 2024-12
- Subjects: Labor laws and legislation -- South Africa , Strikes and lockouts -- Law and legislation -- South Africa , Labor disputes -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68692 , vital:77082
- Description: The main research question for this treatise is to determine whether the South African legal framework provides effective regulations in curbing violent and intractable strikes. To answer the research question, the research explored the evolution of the right to strike in South Africa, analysing its socio-economic impact and the transition from a repressive regime to a democratic one. The study assessed international, regional, and national legal frameworks, focusing on the right to strike and its regulation. Amendments to the LRA, including introducing rules for picketing, mandatory secret ballots for strikes, and advisory arbitration for dysfunctional strikes, were evaluated. The findings indicate that while these amendments represent a significant step in addressing the issues of violent strikes in an employment context, their effectiveness is limited. Persistent challenges include the frequent disregard by trade unions and their members for legal measures. This reflects the deeper socio- economic and political issues. The study suggests that the effectiveness of these legislative changes is hindered by ongoing social and economic inequalities, highlighting the need for broader socio-political solutions beyond legislative amendments. This comprehensive analysis explains the complexities surrounding strike actions in South Africa and the balance between upholding labour rights and maintaining workplace discipline and order. , Thesis (LLM) -- Faculty of Law, School of Mercantile Law, 2024
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- Date Issued: 2024-12
An analysis of employee morale and leadership styles at Benteler automotive Port Elizabeth in the Eastern Cape
- Authors: De Vos, Desmond
- Date: 2024-12
- Subjects: Employee morale , Employee motivation , Leadership -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69553 , vital:77712
- Description: Benteler Automotive in the Eastern Cape has experienced challenges over the past five years due to a high rate of staff turnover at the leadership and management levels. As a result, the morale of the employees in the organisation was affected, resulting in decreased levels of job satisfaction, and productivity, high rates of absenteeism, and staff turnover. Hence, this study was conducted to assess employee morale and leadership styles and examine the impact of motivation, leadership styles, and human resource strategies on staff morale, with the intention of proposing managerial interventions to enhance employee morale within the organisation. The study was conducted within a positivist paradigm, and a quantitative research approach was used to investigate the phenomenon. Data were collected from a sample of 300 employees using a closed-ended questionnaire survey, and a response rate of 93% was achieved. The Statistical Package for Social Science (SPSS) Version 21 was used for analysing and reporting on data in line with the research objectives of the study. The analysed data were reported under descriptive and inferential statistical headings, where frequency of the Likert scale responses, measures of central tendencies, Pearson’s correlations, regression analysis, and analysis of variance (ANOVA) were reported. The results of the study indicates that motivation, leadership styles and human resource strategies have a significantly positive influence on employee morale. On the other hand, the results also indicate, in general, that respondents were indifferent to most of the statements pertaining to leadership styles and human resource strategies. The study recommends that management consider implementing fundamental theoretical human resource strategies to enhance employee morale. These strategies include fostering human relations, providing continuous training opportunities to enhance employee knowledge, promoting new skills development, boosting self-confidence, and addressing concerns about job stability. The results of the study have provided practical and conceptual managerial and academic benefits within the field of management and leadership. A future study on the evaluation of the impact of leadership styles and cultural elements on employee morale is an essential element to consider, as it was not covered in this study. , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business School, 2024
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- Date Issued: 2024-12
Assessing perceptions regarding sustainable growth of taverns in the Gqeberha liquor industry
- Authors: Coutsourides, Peter Dimitri
- Date: 2024-12
- Subjects: Taverns (Inns) -- Port Elizabeth- Eastern Cape -- South Africa , Liquor industry -- Port Elizabeth -- Eastern Cape , Economic development -- Port Elizabeth -- Eastern Cape -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69542 , vital:77710
- Description: This study investigates the sustainable growth of taverns within the informal liquor industry in Gqeberha, South Africa. The informal sector is critical for economic development, employing 60% of the world's population and nearly 30% of South Africa's workforce. This study focuses on taverns, a prevalent form of small, medium, and micro-enterprises in South African townships. Despite their economic significance, these taverns face numerous challenges, including limited access to finance, inadequate infrastructure, regulatory hurdles, and competition from larger formal enterprises. The research explores the historical context of the liquor industry in South Africa, highlighting the legacy of racial inequality and the evolution of regulatory frameworks aimed at balancing industry growth and social equity. In addition, the literature covered topics relating to SMMEs and sustainable growth. The study employs a case study strategy and a qualitative methodology where 12 tavern owners in Gqeberha were interviewed using an interview schedule. The thematic data analysis revealed five main challenges that hinder taverns' sustainable growth, namely finance, government interventions and facilities, competition, the COVID-19 Pandemic and crime. In addition, three measures or themes of sustainable growth were identified for these enterprises, such as economic, social and environmental measures for sustainable growth. Finally, based on the findings, a major contribution of this study is the framework of sustainable growth enablers that were presented to SMMEs, including taverns. This study contributes to the understanding of SMMEs in the informal sector and offers practical recommendations for enhancing the sustainable growth of taverns. By addressing the identified challenges and leveraging the proposed enablers, policymakers and stakeholders can create a more supportive environment for these enterprises, ultimately contributing to broader economic development and poverty reduction in South Africa. The insights gained from this study are intended to inform future policies and support mechanisms that can help these enterprises thrive despite their myriad challenges. , Thesis (MCom) -- Faculty of Business and Economic Sciences, School of Management Sciences, 2024
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- Date Issued: 2024-12
Associations between patterns of wild ungulate patch use, soil carbon and albedo in montane grasslands
- Authors: Mc Gregor, Steven
- Date: 2024-12
- Subjects: Grassland ecology , Global environmental change , Climatic changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69382 , vital:77250
- Description: Grassy ecosystems are essential for human survival, providing key services including food production, water provisioning and moderating climate. Yet, grassy ecosystems remain undervalued due to misconceptions that they are remnants of degraded states of forest – a view that continues to influence climate change policies. This thesis explores the links between wild, free-roaming ungulate grazers and climate drivers, including carbon storage and surface albedo (i.e., reflectance of solar radiation), through their impacts on vegetation and soils. Using black wildebeest (Connochaetes gnou) as a model wild species, I compared their grazing patterns in an Afromontane grassland in the eastern Karoo, South Africa, to those of short-duration cattle grazing systems in similar nearby grasslands (Chapter 2). While short-duration grazing aims to mimic spatiotemporal wild ungulate grazing patterns to supposedly enhance ecosystem functioning and soil carbon stocks, I found that wildebeest had ~50% shorter grazing durations and much shorter rest intervals (1-5 days versus 60-365 days), revealing key differences in grazing patterns that may affect vegetation and climate feedbacks. Next, I examined spatial variations in soil organic carbon (SOC) stocks between grass growth forms that differ in grazing tolerance (Chapter 3). Red grass (Themeda triandra) tussock patches, sensitive to frequent grazing, had higher SOC to a soil depth of 20 cm (61.45 ± 1.59 Mg C·ha-1)than intensively grazed, prostrate-growing Cynodon dactylon grazing lawns (55.43 ± 3.40Mg C·ha-1), likely due to greater shading and soil moisture beneath tussocks which drives microbial decomposition. Seasonal albedo variations were then assessed across distinct grassland patch types among seasons to determine whether albedo varies seasonally at fine patch-scales between grass patches, between shrub and grass patches, and with grazing (Chapter 4). Albedo was lower during the growing season compared to dormancy, and was consistently lower in dwarf shrub (Pentzia incana) encroached patches compared to grass patches. No albedo differences between grazed and less-grazed tussock grass patches of the same species were found, although intensively grazed grazing lawns had consistently higher albedo than most patch types. Finally, I evaluated trade-offs between plant carbon, albedo, and their impacts on radiative forcing (i.e., atmospheric warming/cooling) resulting from patch type changes commonly found in grassy ecosystems (Chapter 5). The loss of perennial grass cover resulted in the highest net positive (warming) effect, mostly due to reduced root biomass. Additionally, shrub encroachment into all patches lowered albedo, but led to negative (cooling) effects from shrub encroachment into bare ground patches due to biomass gains. This thesis challenges current views of grassy landscapes and short-duration grazing systems, emphasizing the need to rethink climate change mitigation strategies to prioritize maintaining heterogeneity, while enhancing carbon sequestration and albedo in grassy ecosystems. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2024
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- Date Issued: 2024-12
Causative factors for poor contractor performance in schools infrastructure projects
- Authors: Baba, Vuyiseka
- Date: 2024-12
- Subjects: Project management , Project management -- Evaluation , School facilities -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68703 , vital:77083
- Description: The public sector infrastructure departments in South Africa rely on construction companies as the major stakeholders in the implementation and maintenance of infrastructure facilities including schools. The Limpopo Province is faced with a serious challenge of underperforming contractors. This in turn affects the Provincial Government’s effective delivery of the much-needed quality infrastructure and facilities. A significant number of schools in the province are faced with infrastructure challenges including sanitation facilities, deteriorating school buildings, storm damage, inadequate infrastructure for special schools, water provision, and general maintenance. This study sought to understand the challenges faced by contractors and further explored whether the effective application of project management methodologies can have a positive impact on improving the current underperformance. Due to the nature of the study, a qualitative research method was employed to collect data. An interpretivist paradigm was adopted as the research philosophy, as it allowed for a flexible and in-depth investigation approach. The study utilized an exploratory research design. The targeted population for the study was carefully selected to obtain relevant and reliable data, and a purposive sampling technique was chosen. Semi-structured interviews were conducted as the primary data collection method, enabling the collection of first-hand information. To ensure accuracy and prevent loss of information, the research process involved recording and transcribing the interviews, using an inductive analysis approach. Eighteen out of the twenty targeted participants were successfully interviewed, resulting in a response rate of 90%. The findings of the study revealed that poor planning, inadequate financial management, inexperienced staff, interference from stakeholders, and a misunderstanding of project scope were among the many factors contributing to poor contractor performance. Furthermore, contractors, particularly Small and Medium Enterprises (SMEs), faced serious challenges during project implementation, including a lack of proper training, budget constraints, and delayed payments from clients. Based on the study's findings, it is recommended that contractors invest in planning and cost management software, including project management methodologies such as Agile, Waterfall, and MS Project. Further, contractors are encouraged to employ adequately skilled and qualified site personnel. Clients should also consider supporting underperforming contractors by pairing them with proven-performing ones to encourage mentorship and other forms of capacity building. , Thesis (MSc) -- Faculty of Engineering, the Built Environment, and Technology, School of Building and Human Settlements Management, 2024
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- Date Issued: 2024-12
Characterization of the molecular mechanism(s) of cannabinoid-induced paraptosis in breast cancer cells
- Authors: de la Harpe, Amy
- Date: 2024-12
- Subjects: Cannabinoids , Cannabinoids -- Therapeutic use , Cancer cells
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68818 , vital:77120
- Description: Natural products have been extensively studied for their anticancer potential, and several widely used anticancer drugs have natural origins. Many studies have demonstrated that natural compounds can induce paraptosis in various tumour cell lines. Paraptosis is a caspase-independent cell death mechanism characterised by cytoplasmic vacuolation arising from the endoplasmic reticulum (ER) and the mitochondria. The molecular mechanism of paraptosis is unclear; however, the literature indicates that dysregulation of calcium signalling plays an important role in paraptosis induction, particularly calcium-mediated signalling between the ER and mitochondria. This study aimed to identify and characterise the mechanism of cell death induced by a phytocannabinoid ratio which induced significant cytoplasmic vacuolation in the MCF7 breast cancer cell line. Several techniques were employed to address the aim of the study. The crystal violet assay was used to detect changes in viability. Several pathway inhibitors, as well as fluorescent staining and analysis using high-content screening (HCS), were used to measure the induction of various cell death mechanisms. Morphological changes were investigated using light and transmission electron microscopy. The phytocannabinoid ratio induced significant cell death and cytoplasmic vacuolation in MCF7 cells; however, the same trend was not observed in the MCF10A non-tumourigenic breast cell line. No cell cycle arrest, apoptosis, necrosis, autophagy, or ferroptosis induction was detected in MCF7 cells, suggesting that an alternative mechanism of cell death was induced. Vacuolation and cell death induced by the phytocannabinoid ratio were inhibited by cycloheximide, suggesting a dependence on protein synthesis, which is characteristic of paraptosis induction. The mechanism of paraptosis induction by the phytocannabinoid ratio was investigated, and it was found that treatment 1) induced ER dilation and mitochondrial swelling; 2) induced significant ER stress, mitochondrial calcium overload, and mitochondrial dysfunction which appeared to be mediated by the voltage-dependent anion channel (VDAC); and 3) significantly impaired all mitochondrial metabolic pathways. Overall, the data demonstrated that paraptosis induced by the cannabinoid ratio is mediated by calcium flux from the ER to the mitochondria. This highlighted a novel mechanism of phytocannabinoid-induced cell death and emphasised the anti-cancer potential of the use of phytocannabinoid ratios as opposed to individual phytocannabinoids, expanding their potential for use as anticancer agents. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
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- Date Issued: 2024-12
Co-generated learnings from hopes-an NRF grant funded university engagement project
- Authors: Quvile, Xola Bulumko
- Date: 2024-12
- Subjects: National Research Foundation (South Africa) , Research grants -- South Africa , Education, Higher -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68366 , vital:76990
- Description: The study had sought to understand the systemic and individual factors that facilitated or inhibited the NRF grant-funded HOPES Project. HOPES collaborated with the Centre for the Community School in the Faculty of Education, and various communities of practice (COPs) in existing school communities in the Eastern Cape Province of South Africa. The project applied a critical participatory action research work to develop a framework for school improvement and in the process contribute to making schools beacons of hope. This research provides insights into how an NRF community engagement grant project team (HOPES) navigated external and internal factors such as COVID-19, rules regulating the grant, and multiple realities of the stakeholders at play. The study was qualitative and used the critical participatory action research design. The study purposively and conveniently sampled seven HOPES Project team members to collect data via focus groups and individual interviews and collected secondary data from NRF HOPES annual project reports. The study findings improved the participants' understanding of their journey, and the co-generated knowledge shed light on how NFR and community engagement grant funders could promote enabling conditions towards achieving individual scholars and collective project outcomes. The study findings provide learnings for the Faculty of Education and the Nelson Mandela University on how extrinsic and intrinsic enablers and barriers can be addressed to optimise a project of this nature. , Thesis (MEd) -- Faculty of Education, School of Postgraduate Education, 2024
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- Date Issued: 2024-12
Collaborative conservation governance in multifunctional landscapes: a contextual analysis of two South African biosphere reserves in the Boland
- Authors: Klaver, Michael
- Date: 2024-12
- Subjects: Unesco -- Programme on Man and the Biosphere , Nature conservation -- South Africa , Biosphere
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68924 , vital:77164
- Description: The Man and the Biosphere Programme (MAB) has been earmarked as one of the most promising ways of tackling the grand challenges of our time and achieving the Sustainable Development Goals. Furthermore, it may have the potential to make positive contributions to one of the most troubling conservation challenges of our time – winning spaces for large carnivores in human-dominated landscapes. This study aims to assess the interpretation and implementation of MAB in two South African Biosphere Reserves (BRs) and explore how BRs as landscape level collaborative initiatives can support Cape leopard conservation in the Boland region of the Western Cape, South Africa. This study is informed by two objectives. First, to understand how the chosen governance strategy and structure of two South African BRs have helped to achieve a context appropriate interpretation and actioning of MAB in the landscape. Second, to understand how landscape-level collaborative conservation initiatives support leopard conservation across a multifunctional landscape. This study takes on a mixed methods approach in which qualitative data takes priority and quantitative data is used for support in an embedded multiple-case study design. The sampling techniques used include purposive sampling of strategically targeted participants, i.e. those governing BRs and those leading the collaborative initiative and snowballing thereon. Data collection included a combination of semi-structure interviews (n=20) (the principal collection method), a review of archival, policy and other documents (n=15), and supported by a mixed methods survey questionnaire (n=5). Data were analysed through thematic and content analysis. Findings suggest that organisations working at a landscape-level utilise forms of adaptive co-management and/or governance as a means to being both adaptive and collaborative. Important themes which emerge include cross-sectoral approaches, networks, leadership and trust for building partnerships, shared visions and ‘filling gaps’ left by, or collaborating with the state to fulfil mutually beneficial objectives. This research provides empirical studies on MAB, important for sharing knowledge across the World Network of BRs, meeting the need for further research on collaborative initiatives in social-ecological systems and conservation programmes for large free-roaming carnivores in multifunctional landscapes. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Science & Management, 2024
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- Date Issued: 2024-12
Comparative study of the effect of iloprost on neuroinflammatory changes in c8-b4 microglial cells and murine model of trypanosomiasis
- Authors: Jacobs, Ashleigh
- Date: 2024-12
- Subjects: Neuroimmunology , DNA -- Methylation , Nervous system -- Degeneration
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68895 , vital:77160
- Description: Neurodegenerative conditions significantly impact well-being and quality of life in individuals with major symptoms including mood disorders, cognitive decline, and psychiatric disturbances, often resulting from neuroinflammation triggered by immune responses to bacterial or parasitic infections such as gram-negative bacteria or Human African Trypanosomiasis. Microglia play a crucial role in both neurotoxicity and cellular processes involved in restoring the neural health. Exploring the therapeutic potential of prostacyclin and its analogues in regulating microglia responses to inflammatory insult and treating Trypanosoma brucei (T.b) infection remains an unexplored area. The aim of this study was to assess the potential neuroprotective effects of Iloprost through comparative analysis of neuroinflammatory responses in both microglial cells exposed to lipopolysaccharide (LPS) and mouse brains infected with T.b brucei. In phase I of this study both resting and LPS treated C8-B4 microglial cells were exposed to varying concentrations of Iloprost. The effects of Iloprost on LPS-induced inflammation were analysed using immunofluorescence to detect microglial activation and differentiate between pro and anti-inflammatory phenotypes. Furthermore, pro and anti-inflammatory cytokine secretion was determined using an ELISA, in addition gene expression analysis was carried out using quantitative polymerase chain reaction (qPCR). Also, DNA methylation status of C8-B4 cells exposed to LPS challenge alone or in combination with various concentrations of Iloprost were determined using bisulfite sequencing technique followed by qPCR. In phase II of the study, a total of twenty-four Albino Swiss male mice (8-10 weeks old) were divided into four treatment groups with 6 mice in each group. All treatment groups except the non-infected control were inoculated with the T.b brucei parasite. One group received a single intraperitoneal injection of Diminazene aceturate (4 mg kg-1)while the remaining group received repeated intraperitoneal injections of Iloprost (200 μg kg-1). On day ten of the study, mouse brains were removed on ice using forceps. The hippocampal tissues were dissected out and processed for quantification of gene expression changes in pro and anti-inflammatory cytokines. Overall, the findings of this study indicate that LPS-induced pro-inflammatory cytokine, TNF-α and IL-1β, secretion and gene expression is down-regulated in C8-B4 microglial cells treated with Iloprost. Furthermore, there was a significant up-regulation in the expression of anti-inflammatory genes, particularly ARG-1, CD206, BDNF and CREB in response to Iloprost treatment following LPS-induced inflammation. This study is also the first to confirm M2 microglial polarization with Iloprost treatment in both resting and LPS treated cells. However, hypermethylation at CREB and BDNF promoter regions was observed 24 hours after Iloprost treatment. Additionally, Iloprost reversed hypomethylation at the BDNF promoter region that had been induced by LPS treatment. The rodent model also indicated a downregulation in the pro-inflammatory cytokine, IL-1β, expression and upregulation of BDNF transcription in T.b brucei infected mice treated with repeated doses of Iloprost. In conclusion, determining the immunomodulatory roles of Iloprost in both in vitro and in vivo models of neuroinflammation could assist in the development of alternative therapy for neurodegenerative disease. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
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- Date Issued: 2024-12
Construction material waste control success model for the construction industry
- Authors: Musah, Abdul Razak
- Date: 2024-12
- Subjects: Construction industry -- Materials management , Construction industry -- Management , Construction industry -- Environmental aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68796 , vital:77114
- Description: Economic sustainability and reconstruction in many economies rely heavily on the construction sector and remain strategic tools for governments and industries worldwide. Excessive construction costs and materials waste among some construction companies are rampant in the construction sector. Most of this waste is poorly managed, thus triggering significant project failure problems, leading to the project stakeholders losing the value of their investment. This concern advocate for effective control of construction material to avoid wastage. The primary objective of this study was to develop a Construction Waste Material Control (CWMC) model to be used by construction project professionals throughout the Project Management (PM) phases: initiation, planning, execution, monitoring, control and closure. Significant literature on construction materials waste, Project Management (PM) processes, and project performance regarding poor waste management and project failure was reviewed. This literature review was conducted to identify factors affecting Construction Waste Material Control (CWMC). These factors were identified and defined together with their respective contributions to the model. A quantitative survey instrument (questionnaire) was used to obtain information from construction sector professionals. A total of 213 questionnaire were received and administered for the study. Structural Equation Model (SEM) SEM was used to analyse the questionnaire data, and thereby assisting the research to clearly define latent constructs statistically significant to the (CWMC) model. The finding was that, the factors identified to have impact on CWMC are Project Initiation Phase Tools (PIPTs), Project Planning Phase Tools (PPPTs), project failure/success factors (PFFs), Project Execution Phase Tools (PEPTs), Project Monitoring and Control Phase Tools (PMCPTs), Construction waste material Control (CWMC) policy and Project Management (PM) competence. The supported hypotheses also authenticated these factors. Introducing the model into the construction sector provides another dimension to CWMC. By establishing the aforementioned factors, the model gives construction professionals precise CWMC predictive accuracy. As the model reliability test of 0.734 to 0.972 satisfied the theoretical threshold and goodness of fit of 0.912 of Normed fit index respectively. The model is the first of its kind and contributes significantly to the construction sector’s existing body of knowledge. , Thesis (PhD) -- Faculty of Engineering, the Built Environment, and Technology, School of Built Environment and Civil Engineering, 2024
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- Date Issued: 2024-12
Development of density-dependent and density-independent competition models to understand single tree growth responses in eucalyptus stands
- Authors: Kepe, Lulama Patrick
- Date: 2024-12
- Subjects: Forests and forestry -- Mathematical models , Trees -- Growth -- Mathematical models , Eucalyptus grandis
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68906 , vital:77161
- Description: In commercial forest production, predicted yields based on mensuration recommendations are seldom realized, and minimal silvicultural standards based on results from trial blocks do not account for the relationship between initial planting density and final stocking. Growth models designed to investigate management options must employ competition indices to provide reliable predictions under extreme silviculture. The competition a tree experiences until the day of harvest is a strong driver for quality and volume of timber. Hence, this turns the regulation of competition into an integral part of silviculture. According to Little et al. (2003), it is known that, at some stage after canopy closure, individual trees in a stand will begin to compete for resources. To investigate competition amongst individual trees, a re-purposed Bayesian mixed effects model, similar in characteristics to a Sire model used for estimating breeding values, is proposed. In animal breeding models, the Sire Model allows for the inclusion of a numerator relationship matrix containing inbreeding coefficients. In a similar way, documented competition indices are included in the model in an attempt to estimate posterior probabilities of specifically selected individual trees being the strongest growers during different stages in the tree growth cycle. Estimated tree growth indices were determined and used to make probability statements in order to rank the individually selected trees based on the amount of growth observed. A tree growth index is a measurement of a tree’s average growth performance in relation to the average growth performance of all trees on the same plot. As different competition indices are introduced into the model, changes in the probabilities are observed and compared to what is visually observed on the plot, i.e. if the tree with the highest probability of being the strongest grower, is actually not necessarily the largest tree of the group of selected trees, but rather the tree that presented the largest amount of growth of the selected group of trees during that specific growth season. From a randomly selected plot, a group of neighboring trees were randomly selected. Four specifically selected target trees were then identified from this group of 25 selected trees, and analyzed. For this discussion, for example, from our randomly selected group of 25 neighboring trees, tree 54, tree 56, tree 86, and tree 88 were specifically selected for further analysis. For these specifically selected trees, marginal posterior densities for the variance components and random effects were then estimated using the Gibbs Sampler, where competition between the trees was assumed present, as well as for the case where it was assumed that no competition takes place between the individually selected trees. In the latter case, an identity matrix was utilized in the Gibbs sampling algorithm where it was assumed that there is no competition between the trees. Both a distance independent competition index (Lorimer,1983), and a distance dependent index (Hegyi, 1974), were used to generate the numerator relationship matrix A that was used by the Gibbs sampling algorithm in the case where competition was assumed between individual trees. Also a new distance dependent index was proposed and tested in this study as well. Results from the selected plot, with a 1500 stems per hectare (sph) density, indicated that there were no significant differences in the average growth between these selected trees. However, the marginal posterior densities of the fixed effects indicated that there was a significant difference in the average growth rates between the base level conditions and other levels determined for each of these fixed effect factors for the selected trees, since their 95% equal tails credibility intervals, did not contain zero. This therefore indicated that the specific treatment applied on the plot, had a significant effect on the individual tree growth. Results when competition was assumed present, revealed that the estimated marginal posterior densities for the error variance as well as tree variance, were severely positively skewed. , Thesis (PhD) -- Faculty of Science, School of Natural Resource Science & Management, 2024
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- Date Issued: 2024-12
Experiences of accoucheurs caring for women during confinement in public maternity units in the O.R. Tambo District Municipality
- Authors: Wabane, Malibongwe
- Date: 2024-12
- Subjects: Midwifery -- South Africa -- Eastern Cape , Labor (Obstetrics) -- South Africa -- Eastern Cape , Hospital care -- South Africa -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69782 , vital:78034
- Description: Midwifery is a health science profession that deals with women in their confinement period, including the caring of new-borns. Historically, the midwifery profession has evolved from being a female-dominated profession to an occupation followed by both males and females. However, accoucheurs working in public maternity units face various challenges, such as a lack of support and discrimination from their female counterparts and women during confinement. These challenges are based mainly on tradition, religion, and the history of midwifery, a female-dominant profession. The potential effects of these challenges can be seen when women during confinement refuse maternal care from accoucheurs. As a result of this prejudice, accoucheurs may be unable to practice their midwifery skills and may choose other disciplines involving less intimate care. Such acts of refusal of care by women during confinement and these various challenges faced by accoucheurs in public maternity units are common in rural areas. The O.R. Tambo District Municipality (ORTDM) comprises predominantly of rural areas where culture and tradition play an essential role in the community’s life. This study employed a qualitative research design with an explorative, descriptive, and contextual approach to gain a deeper understanding of the experiences of accoucheurs caring for women during confinement in public maternity units in the ORTDM. The purpose of this study was to explore and describe the experiences of accoucheurs caring for women during confinement in public maternity units in the ORTDM to make recommendations to nursing managers regarding the provision of optimal support to accoucheurs working in the rural Eastern Cape. The target population of the study was all accoucheurs working in public maternity units in the ORTDM. Snowball sampling, a non-probability sampling method, was used to select participants who met the inclusion criteria and were voluntarily willing to participate in the study. The data collection method used was one-on-one semistructured interviews, which were also captured using a digital voice recorder. Field notes were used during data collection to enhance the researcher’s understanding of accoucheurs’ nonverbal experiences. The sample size, which was 12 accoucheurs, was determined by data saturation. Data was transcribed verbatim, and Braun and Clark’s (2006) steps of reflexive thematic analysis (RTA) were used as a framework for data analysis. Lincoln and Guba’s model of trustworthiness was used to maintain the quality of the study by employing credibility, transferability, confirmability, and dependability (Lincoln and Guba, 1985). The ethical principles that guided this study with human participants were the principles of respect for persons, beneficence, non-maleficence, and justice as outlined in the Belmont Report and its application (2015:14) Three main themes emerged from the data analysis, namely: • Theme 1: Accoucheurs experienced specific factors that enabled them to care for women during confinement in public maternity units. • Theme 2: Accoucheurs identified barriers that disabled them in caring for women during confinement in public maternity units. • Theme 3: Recommendations for nurse managers by accoucheurs to optimize their ability to care for women during confinement in public maternity units. Direct quotations from the raw data and relevant literature supported the research study results and were discussed in-depth in Chapter Four. Various recommendations were discussed in Chapter Five of this study that present options for nurse managers to optimize the support for accoucheurs who were caring for women during confinement in public maternity units.Midwifery is a health science profession that deals with women in their confinement period, including the caring of new-borns. Historically, the midwifery profession has evolved from being a female-dominated profession to an occupation followed by both males and females. However, accoucheurs working in public maternity units face various challenges, such as a lack of support and discrimination from their female counterparts and women during confinement. These challenges are based mainly on tradition, religion, and the history of midwifery, a female-dominant profession. The potential effects of these challenges can be seen when women during confinement refuse maternal care from accoucheurs. As a result of this prejudice, accoucheurs may be unable to practice their midwifery skills and may choose other disciplines involving less intimate care. Such acts of refusal of care by women during confinement and these various challenges faced by accoucheurs in public maternity units are common in rural areas. The O.R. Tambo District Municipality (ORTDM) comprises predominantly of rural areas where culture and tradition play an essential role in the community’s life. This study employed a qualitative research design with an explorative, descriptive, and contextual approach to gain a deeper understanding of the experiences of accoucheurs caring for women during confinement in public maternity units in the ORTDM. The purpose of this study was to explore and describe the experiences of accoucheurs caring for women during confinement in public maternity units in the ORTDM to make recommendations to nursing managers regarding the provision of optimal support to accoucheurs working in the rural Eastern Cape. The target population of the study was all accoucheurs working in public maternity units in the ORTDM. Snowball sampling, a non-probability sampling method, was used to select participants who met the inclusion criteria and were voluntarily willing to participate in the study. The data collection method used was one-on-one semistructured interviews, which were also captured using a digital voice recorder. Field notes were used during data collection to enhance the researcher’s understanding of accoucheurs’ nonverbal experiences. The sample size, which was 12 accoucheurs, was determined by data saturation. Data was transcribed verbatim, and Braun and Clark’s (2006) steps of reflexive thematic analysis (RTA) were used as a framework for data analysis. Lincoln and Guba’s model of trustworthiness was used to maintain the quality of the study by employing credibility, transferability, confirmability, and dependability (Lincoln and Guba, 1985). The ethical principles that guided this study with human participants were the principles of respect for persons, beneficence, non-maleficence, and justice as outlined in the Belmont Report and its application (2015:14) Three main themes emerged from the data analysis, namely: • Theme 1: Accoucheurs experienced specific factors that enabled them to care for women during confinement in public maternity units. • Theme 2: Accoucheurs identified barriers that disabled them in caring for women during confinement in public maternity units. • Theme 3: Recommendations for nurse managers by accoucheurs to optimize their ability to care for women during confinement in public maternity units. Direct quotations from the raw data and relevant literature supported the research study results and were discussed in-depth in Chapter Four. Various recommendations were discussed in Chapter Five of this study that present options for nurse managers to optimize the support for accoucheurs who were caring for women during confinement in public maternity units. , Thesis (MCur) -- Faculty of Health Sciences, School of Clinical Care & Medicinal Sciences, 2024
- Full Text:
- Date Issued: 2024-12
Exploiting continuous flow technology to develop synthetic processes for active pharmaceutical ingredients: prilocaine and betrixaban
- Authors: Sagandira, Mellisa Brenda
- Date: 2024-12
- Subjects: Pharmaceutical technology , Drug development , Pharmaceutical biotechnology
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69464 , vital:77259
- Description: The dilemma of providing affordable health care has long haunted African countries due to the continuously growing disease burden as the population increases. Africa accounts for only 3 % of global drug production and imports over 90 % of its life saving medicines at a yearly cost of US$14 billion according to the African Development Bank (AfDB). Attaining Africa’s Agenda 2063, Universal Health Coverage (UHC) and Sustainable development Goal 3 (SDG3) aspirations of ensuring healthy and well-nourished citizens remain difficult given this situation. This over-dependence on imports has led to drug shortages, high pricing, poor quality medicines and unguaranteed supply chains, thus there still remains an unmet need. The advent of the COVID-19 pandemic worsened this situation as many countries curbed exports resulting in a crisis across the continent. Africa experienced high demand of medicines compared to prepandemic level, which threatened the health care of the continent. This situation can be addressed by establishment of local pharmaceutical manufacturing capability through adoption of advanced manufacturing technologies such as continuous flow chemistry. In this research, we therefore seek to exploit continuous flow chemistry, an enabling technology to develop safe and efficient synthetic processes for active pharmaceutical ingredients: prilocaine and betrixaban as case studies. To the best of our knowledge there is no reported work on synthetic processes towards prilocaine and betrixaban under continuous flow conditions, thus this research extensively unearthed interesting continuous flow processes towards these APIs. In a broader view, establishment of local APIs production will ease Africa’s over-dependence on imports, which will in turn address issues such as drug shortages, poor quality and high pricing. This work aims to demonstrate how continuous flow technology can be useful for the establishment of local APIs manufacturing, which will inherently revolutionize medicines availability, affordability and accessibility to the greater population. Chapter one begins with a brief overview on Africa’s disease burden and pharmaceutical supply chain. Continuous flow chemistry is introduced with its benefits and limitations discussed. Lastly a mini review of examples of APIs synthesised in continuous flow is presented. In chapter two, we demonstrate how continuous flow technology can be exploited to develop synthetic processes towards prilocaine as our first case study. This chapter clearly demonstrates how highly exothermic nitration reactions can be safely handled by using continuous flow systems. Additionally, the use of a micro structured mixer facilitated biphasic nitration of toluene to be carried out with enhanced mass transfer, which inherently led to improved selectivity of ortho-nitrotoluene (preferred isomer) to 79 % compared to 59 % achieved in batch mode. A two-step synthesis of ortho-toluidine via biphasic nitration of toluene and nitroreduction was demonstrated with the incorporation of a Zaiput liquid/liquid separator to facilitate in-line separation. Comprehensive optimisation studies were carried out in flow. Prilocaine was synthesised in 74 % overall yield in a 13.6 min residence time for all processes. In chapter three, we demonstrate how continuous flow technology can be exploited to develop synthetic processes towards betrixaban. Synthetic routes towards betrixaban feature two amide bond formation transformations. Pleasingly, we explored multistep chemistry to develop amide synthesis protocols in flow without interruption for work up and isolation. An overall yield of 92 % was achieved to get to key intermediate to betrixaban in 8 min total residence time. Chapter four demonstrates the robustness of developed amide synthesis protocols, a key transformation in synthetic routes towards prilocaine and betrixaban in continuous flow systems. A total of 22 amides were synthesised via flow multistep processes with isolated yields greater than 82 %. All the compounds were characterized using infrared spectroscopy (IR), and nuclear magnetic resonance (NMR). Quantification of the reaction mixtures were carried out using highperformance liquid chromatography (HPLC) and gas chromatography (GC). , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12