Investigating thermal physiology as a tool to improve the release efficacy of insect biological control agents
- Authors: Griffith, Tamzin Camilla
- Date: 2018
- Subjects: Aquatic weeds -- Biological control , Water hyacinth -- Biological control , Insects -- Physiology , Miridae -- Effect of low temperatures on , Cold adaptation , Insects as biological pest control agents , Eccritotarsus catarinensis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63301 , vital:28391
- Description: Biological control is commonly used for the control of invasive aquatic weeds, which often involves the release of multiple host-specific agents. Releasing multiple agents has inherent safety concerns as the introduction of each new agent is associated with risks, but is often required to improve control where establishment is limited. Climatic incompatibility between the agent’s thermal physiology and its introduced range often causes agents to fail to establish. However, it has been suggested that the thermal physiology of insects is plastic. Therefore, the potential to manipulate their thermal physiologies before releasing them into the field needs to be explored; reducing the need to release additional agents, thereby ensuring the safety of biological control. This thesis therefore aimed to firstly, determine whether season and locality influenced the thermal physiology of two field populations of a water hyacinth (Eichhornia crassipes) control agent, the mirid Eccritotarsus catarinensis; one collected from the hottest establishment site, and one collected from the coldest establishment site in South Africa. Their thermal physiology was significantly influenced by season and not by the sites’ climate, suggesting their thermal physiology is plastic under field conditions. Secondly, the classical method of determining the lower critical thermal limit (CTmin), and a new respirometry method of determining this limit, compared the thermal physiology of two Eccritotarsus species reared in quarantine. Eccritotarsus catarinensis was significantly more cold tolerant than the more recently released Eccritotarsus eichhorniae, despite similar maintenance conditions, and as such, was used to establish whether cold hardening under laboratory conditions was possible. Successfully cold hardened E. catarinensis had a significantly lower CTmin compared to the field cold acclimated population, suggesting that cold hardening of agents could be conducted before release to improve their cold tolerance and increase their chances of establishment, allowing for further adaptation to colder climates in the field to occur. Increasing establishment of the most effective agents will decrease the number of agents needed in a biological control programme, thus encouraging a more parsimonious approach to biological control.
- Full Text:
- Date Issued: 2018
- Authors: Griffith, Tamzin Camilla
- Date: 2018
- Subjects: Aquatic weeds -- Biological control , Water hyacinth -- Biological control , Insects -- Physiology , Miridae -- Effect of low temperatures on , Cold adaptation , Insects as biological pest control agents , Eccritotarsus catarinensis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63301 , vital:28391
- Description: Biological control is commonly used for the control of invasive aquatic weeds, which often involves the release of multiple host-specific agents. Releasing multiple agents has inherent safety concerns as the introduction of each new agent is associated with risks, but is often required to improve control where establishment is limited. Climatic incompatibility between the agent’s thermal physiology and its introduced range often causes agents to fail to establish. However, it has been suggested that the thermal physiology of insects is plastic. Therefore, the potential to manipulate their thermal physiologies before releasing them into the field needs to be explored; reducing the need to release additional agents, thereby ensuring the safety of biological control. This thesis therefore aimed to firstly, determine whether season and locality influenced the thermal physiology of two field populations of a water hyacinth (Eichhornia crassipes) control agent, the mirid Eccritotarsus catarinensis; one collected from the hottest establishment site, and one collected from the coldest establishment site in South Africa. Their thermal physiology was significantly influenced by season and not by the sites’ climate, suggesting their thermal physiology is plastic under field conditions. Secondly, the classical method of determining the lower critical thermal limit (CTmin), and a new respirometry method of determining this limit, compared the thermal physiology of two Eccritotarsus species reared in quarantine. Eccritotarsus catarinensis was significantly more cold tolerant than the more recently released Eccritotarsus eichhorniae, despite similar maintenance conditions, and as such, was used to establish whether cold hardening under laboratory conditions was possible. Successfully cold hardened E. catarinensis had a significantly lower CTmin compared to the field cold acclimated population, suggesting that cold hardening of agents could be conducted before release to improve their cold tolerance and increase their chances of establishment, allowing for further adaptation to colder climates in the field to occur. Increasing establishment of the most effective agents will decrease the number of agents needed in a biological control programme, thus encouraging a more parsimonious approach to biological control.
- Full Text:
- Date Issued: 2018
The effect of shade on the biological control of Salvinia molesta D.S. Mitchell [Salviniaceae] by the weevil, Cyrtobagous salviniae Calder and Sands [Curculionidae]
- Authors: Maseko, Zolile
- Date: 2017
- Subjects: Salvinia molesta , Aquatic weeds -- Biological control , Beetles , Insects as biological pest control agents
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4808 , vital:20726
- Description: Salvinia molesta, a floating aquatic weed, is a global menace in many water bodies and waterways. The weed disrupts the ecological balance wherever it invades and also has wide ranging economic and health impacts. Its impact has resulted in the need to control it, and while chemical and mechanical control are often ineffective, biological control by the weevil Cyrtobagous salviniae is largely successful. However, in many parts of the world, including South Africa, biological control of S. molesta has been less effective where the weed grows as an understory species. Shallow and shaded waters characteristically found at the margins of water bodies provide a refuge for S. molesta. Therefore, the aim of the study was to determine the effect of shade on the efficacy of biological control of S. molesta. Investigations into the problems associated with control in the shade were carried out in a greenhouse and in the field. In controlled greenhouse experiments, plants were grown at high and low nutrient levels in individual mesocosms, at three varying levels of shade, where half the mesocosms were inoculated with C. salviniae. Plants in high nutrient conditions were significantly more productive in terms of biomass accumulation, compared to those grown at low nutrient levels, in both the absence and presence of herbivory at all levels of shade tested. Plants grown in the shade and in high nutrient conditions had significantly higher quality compared to the ones exposed to full sun. Higher plant quality in the shade consequently resulted in accelerated fecundity for C. salviniae resulting in significantly higher weevil populations. Furthermore, less damage was recorded on plants in the shade, possibly due to the high nitrogen concentrations which may have deterred grazing by C. salviniae. However, in the absence of shading, plants were of lower quality and consequently sustained more damage from herbivores despite lower weevil populations. In contrast to the greenhouse, there were no differences in biomass recorded in the field at two nutrient levels and in the presence of herbivory for both shade and open sites. Nutrients added to high nutrient treatment quadrats diffused evenly across the water body resulting in uniform nutrient distribution, hence uniform plant biomass and carbon-nitrogen ratio. Despite the lack of statistical differences, more weevils were found in the full sun plots, while modest populations were recorded in the shade. Higher weevil populations consequently led to more damage in the sun, a situation that has been observed in most field sites in South Africa. The results therefore suggest that nutrients were pivotal in plant growth compared to light regimes (amount of PAR). Plant quality significantly influenced weevil populations and plant damage in both studies, demonstrating that plant nitrogen plays a crucial role in the plant- herbivore system. Furthermore, in both the greenhouse and field, despite lack of statistical differences, plants exposed to full sunlight suffered more damage than shade plants, pointing towards better control in the sun compared to the shade, suggesting that there is greater preference of sun-exposed plants for grazing by the weevils. The results from this study add to the growing body of literature that plant quality is a major factor in determining the success of aquatic weed biological control programmes.
- Full Text:
- Date Issued: 2017
- Authors: Maseko, Zolile
- Date: 2017
- Subjects: Salvinia molesta , Aquatic weeds -- Biological control , Beetles , Insects as biological pest control agents
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4808 , vital:20726
- Description: Salvinia molesta, a floating aquatic weed, is a global menace in many water bodies and waterways. The weed disrupts the ecological balance wherever it invades and also has wide ranging economic and health impacts. Its impact has resulted in the need to control it, and while chemical and mechanical control are often ineffective, biological control by the weevil Cyrtobagous salviniae is largely successful. However, in many parts of the world, including South Africa, biological control of S. molesta has been less effective where the weed grows as an understory species. Shallow and shaded waters characteristically found at the margins of water bodies provide a refuge for S. molesta. Therefore, the aim of the study was to determine the effect of shade on the efficacy of biological control of S. molesta. Investigations into the problems associated with control in the shade were carried out in a greenhouse and in the field. In controlled greenhouse experiments, plants were grown at high and low nutrient levels in individual mesocosms, at three varying levels of shade, where half the mesocosms were inoculated with C. salviniae. Plants in high nutrient conditions were significantly more productive in terms of biomass accumulation, compared to those grown at low nutrient levels, in both the absence and presence of herbivory at all levels of shade tested. Plants grown in the shade and in high nutrient conditions had significantly higher quality compared to the ones exposed to full sun. Higher plant quality in the shade consequently resulted in accelerated fecundity for C. salviniae resulting in significantly higher weevil populations. Furthermore, less damage was recorded on plants in the shade, possibly due to the high nitrogen concentrations which may have deterred grazing by C. salviniae. However, in the absence of shading, plants were of lower quality and consequently sustained more damage from herbivores despite lower weevil populations. In contrast to the greenhouse, there were no differences in biomass recorded in the field at two nutrient levels and in the presence of herbivory for both shade and open sites. Nutrients added to high nutrient treatment quadrats diffused evenly across the water body resulting in uniform nutrient distribution, hence uniform plant biomass and carbon-nitrogen ratio. Despite the lack of statistical differences, more weevils were found in the full sun plots, while modest populations were recorded in the shade. Higher weevil populations consequently led to more damage in the sun, a situation that has been observed in most field sites in South Africa. The results therefore suggest that nutrients were pivotal in plant growth compared to light regimes (amount of PAR). Plant quality significantly influenced weevil populations and plant damage in both studies, demonstrating that plant nitrogen plays a crucial role in the plant- herbivore system. Furthermore, in both the greenhouse and field, despite lack of statistical differences, plants exposed to full sunlight suffered more damage than shade plants, pointing towards better control in the sun compared to the shade, suggesting that there is greater preference of sun-exposed plants for grazing by the weevils. The results from this study add to the growing body of literature that plant quality is a major factor in determining the success of aquatic weed biological control programmes.
- Full Text:
- Date Issued: 2017
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