- Title
- Finding Frankenflora: investigating hybridisation between local and introduced Protea species at the Van Stadens Wildflower Reserve
- Creator
- Macqueen, Timothy
- Subject
- Proteaceae -- South Africa
- Subject
- Proteaceae Trees -- South Africa Hybridization
- Date Issued
- 2019
- Date
- 2019
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10948/40724
- Identifier
- vital:36230
- Description
- This study provides an overview of hybridisation and methods to detect it in plants. This is documented in chapter 1. Another aim was to identify hybridisation between local and non-local species in the Proteaceae. This is covered in chapter 2. Finally the third aim was to develop a cheap and rapidly working method of detecting hybrids in this system. This is detailed in chapter 3. The model system for this research is the Van Stadens Wildflower Reserve. In 1984 the reserve manager introduced the non-local species, Protea susannae from Cape Agulhas. This represents a long-distance dispersal of over 600 km. This study is focused on looking at the impacts of the introduction of P. susannae on the local species P. eximia. I use genetic and morphological methods to detect hybrids. Hybridisation and the methods implemented to identify it are reviewed and discussed (Chapter 1). Morphological traits of the two species and direct sequencing of the nuclear ITS and chloroplast regions are compared (Chapter 2). This includes notes on the detection of hybrids and the potential for cryptic hybrids. I also focussed on testing of two rapid and low-cost techniques for detecting hybrids using Species Specific Primers (SSP’s) and High Resolution Melt (HRM). The Sanger sequencing method and ITS detected cryptic hybrids. The HRM was able to detect hybrids when comparing F1 putative hybrids to a ‘pure’ parent populations, but was not able to detect between a ‘pure’ parent population and F3 or F4 putative hybrids in the Van Stadens system. The SSP’s had the highest hybrid detection rate. Genetic methods, specifically the SSP’s were found to work well to detect hybrids in an environment where a hybrid swarm has taken place. The maps produced from this thesis will be useful for determining the distribution of hybrids in the reserve and for other similar hybrid systems. Long distance dispersal of Cape species may lead to the loss of genetic diversity or species as local and non-local hybridisation may occur, similar to the hybridisation between P. susannae and P. eximia in the Van Stadens Wild Flower Reserve. Genetic methods were shown to be superior to morphological hybrid detection (i.e. Sanger sequencing and SSP’s). The SSP approach tested here, which is dependent on the presence of fixed species specific SNP’s (Single Nucleotide Polymorphisms), could offer a rapid and effective method to explore hybridisation through space and time.
- Format
- 84 leaves
- Format
- Publisher
- Nelson Mandela University
- Publisher
- Faculty of Science
- Language
- English
- Rights
- Nelson Mandela University
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| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Timothy Macqueen.pdf | 2 MB | Adobe Acrobat PDF | View Details Download |