Uphando ngenxaxheba yemithombo yosasazo ekwakheni nasekuqambeni isigama esitsha: lugxile kunomathotholo, uMhlobo Wenene kwinkqubo iBreakfast eyondlayo ekuseni
- Authors: Ntlantsana, Likona
- Date: 2024-04
- Subjects: Sociolinguistics -- South Africa -- Eastern Cape , Language and languages—Phonetic transcriptions , Xhosa language--Grammar
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63451 , vital:73367
- Description: Olu phando luhlola amandla emithombo yosasazo ekwakheni nasekuqambeni isigama. Lukwenze oku ngokuphanda ngendlela abantu abawabona ngayo amagama amatsha esiXhosa aveliswe kunomathotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni. Esi sifundo sikhangela ukwamkeleka kwala magama kuluntu. Oku kwenziwe ngokuthi kwenziwe udliwanondlebe lobuso ngobuso leqela elingqalileyo oluthe ngqo nabasasazi kunye nabaphulaphuli.Uphando lungqiyame lwaqamela ngendlela yophando edibanisa uphando olujolise kumanani nobalo kunye nophando olujolise kwinkcazo nakubunjani. Olu phando lwenziwe kwiidolophu ezimbini, iTsomo neGqeberha. Zombini ezi dolophu zikwiphondo leMpuma Koloni, eMzantsi Afrika apho abahlali abaninzi bathetha isiXhosa. ITsomo yidolophu esemaphandleni enezinga eliphantsi labantu abathetha ezinye iilwimi ngaphandle kwesiXhosa. Ngoko ke isiXhosa lolona lwimi lutshilayo eTsomo. EGqeberha kulapho Umhlobo Wenene ukhoyo. Ukusebenzisa abantu abasuka kwiindawo ezahlukeneyo kuveza iimbono ezahlukileyo ezithe zanceda ukuphendula iinjongo zolu phando. Injongo yolu phando kukukhangela inxaxheba yonomathotholo, Umhlobo Wenene ekukhuliseni ulwimi lwesiXhosa lugxile kwinkqubo iBreakfast Eyondlayo Ekuseni (BEE). Uphando luza kujolisa kumagama aveliswe kunomathotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni. Iziphumo zophando kufikelelwe kuzo ngokuthi kuthelekiswe iimpendulo zabo kuvele isisombululo sengxaki yophando kuzo. Oku kuthe kwanceda ukuphendula umbuzo wophando ofuna ukuqonda ukuba ingaba unomthotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni udlala inxaxheba ekhukhuliseni ulwimi lwesiXhosa? Kuyabonakala okokuba ngokwendlela asetyenziswa ngayo amagama aveliswe ngabasasazi kwinkqubo iBreakfast Eyondlayo Ekuseni abaphulaphuli bayawuvuyela kwaye bawaphindisela kubasasazi. , Thesis (MA) -- Faculty of Humanities, School of Language, Media and Communication, 2024
- Full Text:
- Date Issued: 2024-04
- Authors: Ntlantsana, Likona
- Date: 2024-04
- Subjects: Sociolinguistics -- South Africa -- Eastern Cape , Language and languages—Phonetic transcriptions , Xhosa language--Grammar
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63451 , vital:73367
- Description: Olu phando luhlola amandla emithombo yosasazo ekwakheni nasekuqambeni isigama. Lukwenze oku ngokuphanda ngendlela abantu abawabona ngayo amagama amatsha esiXhosa aveliswe kunomathotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni. Esi sifundo sikhangela ukwamkeleka kwala magama kuluntu. Oku kwenziwe ngokuthi kwenziwe udliwanondlebe lobuso ngobuso leqela elingqalileyo oluthe ngqo nabasasazi kunye nabaphulaphuli.Uphando lungqiyame lwaqamela ngendlela yophando edibanisa uphando olujolise kumanani nobalo kunye nophando olujolise kwinkcazo nakubunjani. Olu phando lwenziwe kwiidolophu ezimbini, iTsomo neGqeberha. Zombini ezi dolophu zikwiphondo leMpuma Koloni, eMzantsi Afrika apho abahlali abaninzi bathetha isiXhosa. ITsomo yidolophu esemaphandleni enezinga eliphantsi labantu abathetha ezinye iilwimi ngaphandle kwesiXhosa. Ngoko ke isiXhosa lolona lwimi lutshilayo eTsomo. EGqeberha kulapho Umhlobo Wenene ukhoyo. Ukusebenzisa abantu abasuka kwiindawo ezahlukeneyo kuveza iimbono ezahlukileyo ezithe zanceda ukuphendula iinjongo zolu phando. Injongo yolu phando kukukhangela inxaxheba yonomathotholo, Umhlobo Wenene ekukhuliseni ulwimi lwesiXhosa lugxile kwinkqubo iBreakfast Eyondlayo Ekuseni (BEE). Uphando luza kujolisa kumagama aveliswe kunomathotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni. Iziphumo zophando kufikelelwe kuzo ngokuthi kuthelekiswe iimpendulo zabo kuvele isisombululo sengxaki yophando kuzo. Oku kuthe kwanceda ukuphendula umbuzo wophando ofuna ukuqonda ukuba ingaba unomthotholo, Umhlobo Wenene kwinkqubo iBreakfast Eyondlayo Ekuseni udlala inxaxheba ekhukhuliseni ulwimi lwesiXhosa? Kuyabonakala okokuba ngokwendlela asetyenziswa ngayo amagama aveliswe ngabasasazi kwinkqubo iBreakfast Eyondlayo Ekuseni abaphulaphuli bayawuvuyela kwaye bawaphindisela kubasasazi. , Thesis (MA) -- Faculty of Humanities, School of Language, Media and Communication, 2024
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- Date Issued: 2024-04
Value management in government construction projects in South Africa
- Mngeni- Nobala, Thandokazi Yvonne
- Authors: Mngeni- Nobala, Thandokazi Yvonne
- Date: 2024-04
- Subjects: Construction projects -- Management , Project management -- South Africa , Construction industry -- South Africa , Value analysis (Cost control)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/64673 , vital:73845
- Description: The public sector construction industry aims to deliver infrastructure services to its client satisfactorily. However, the public sector is notorious for delivering projects that are over budget, finish years behind schedule, and are of poor quality. Value management is one way to address these challenges. This research aimed to examine the level of awareness and implementation of value management within the public sector and the benefits thereof. In this qualitative study, interviews were employed to gain insight into the level of awareness of value management within the public sector department in the Eastern Cape. A total of ten professionals in the public sector working for the Department of Public Works were purposively selected to be interviewed and the secondary data was sought through document analysis . The data was thematically analysed. The South African government requires a strategic change that will help improve awareness of value management in government infrastructure projects in South Africa which will pave the way for the full implementation of VM. The study found that the obstacle to adopting value management for public projects was the lack of knowledge and practice of value management. It was also found that the respondents were keen to implement value management and saw it as part of an important step in the planning stage of a project as it would save the department time and money. In order to improve the performance of construction projects, there is a need to adopt innovative ideas and solutions that will work for South African construction industry. Value management is one way to improve the performance of construction projects. VM should be considered at a strategic level in public sector departments to improve its applicability to meet client’s objectives. The major goal of the VM is to ensure that projects are delivered to the best function at the lowest total life cost achievable. The satisfaction of clients is a key factor of construction project success. Value Management strives to address problems which may arise such as budgetary constraints trying to decrease unnecessary costs towards the project but without causing changes to quality of the finished product. Value management is believed to be a cost-cutting exercise as perceived by many but the most importantly it focuses on function of an element or project. This means that the project is procured at the lowest possible cost by utilizing multiple cost control mechanisms without compromising the project's value and function. Value management has proven to be a practical management strategy for improving value for money, creating a better focus on project objectives, working toward more effective design, and identifying wasteful expenses due to unneeded design, material, labor, and machine. To optimise the usage of value management and to improve project performance, there is a need to adopt new concepts like value management that will work for the South African construction sector. , Thesis (MSc) -- Faculty of Engineering, the Built Environment, and Technology, School of Built Environment and Civil Engineering, 2024
- Full Text:
- Date Issued: 2024-04
- Authors: Mngeni- Nobala, Thandokazi Yvonne
- Date: 2024-04
- Subjects: Construction projects -- Management , Project management -- South Africa , Construction industry -- South Africa , Value analysis (Cost control)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/64673 , vital:73845
- Description: The public sector construction industry aims to deliver infrastructure services to its client satisfactorily. However, the public sector is notorious for delivering projects that are over budget, finish years behind schedule, and are of poor quality. Value management is one way to address these challenges. This research aimed to examine the level of awareness and implementation of value management within the public sector and the benefits thereof. In this qualitative study, interviews were employed to gain insight into the level of awareness of value management within the public sector department in the Eastern Cape. A total of ten professionals in the public sector working for the Department of Public Works were purposively selected to be interviewed and the secondary data was sought through document analysis . The data was thematically analysed. The South African government requires a strategic change that will help improve awareness of value management in government infrastructure projects in South Africa which will pave the way for the full implementation of VM. The study found that the obstacle to adopting value management for public projects was the lack of knowledge and practice of value management. It was also found that the respondents were keen to implement value management and saw it as part of an important step in the planning stage of a project as it would save the department time and money. In order to improve the performance of construction projects, there is a need to adopt innovative ideas and solutions that will work for South African construction industry. Value management is one way to improve the performance of construction projects. VM should be considered at a strategic level in public sector departments to improve its applicability to meet client’s objectives. The major goal of the VM is to ensure that projects are delivered to the best function at the lowest total life cost achievable. The satisfaction of clients is a key factor of construction project success. Value Management strives to address problems which may arise such as budgetary constraints trying to decrease unnecessary costs towards the project but without causing changes to quality of the finished product. Value management is believed to be a cost-cutting exercise as perceived by many but the most importantly it focuses on function of an element or project. This means that the project is procured at the lowest possible cost by utilizing multiple cost control mechanisms without compromising the project's value and function. Value management has proven to be a practical management strategy for improving value for money, creating a better focus on project objectives, working toward more effective design, and identifying wasteful expenses due to unneeded design, material, labor, and machine. To optimise the usage of value management and to improve project performance, there is a need to adopt new concepts like value management that will work for the South African construction sector. , Thesis (MSc) -- Faculty of Engineering, the Built Environment, and Technology, School of Built Environment and Civil Engineering, 2024
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- Date Issued: 2024-04
Youth participation in sports: Reconsidering the lost culture of Indigenous games in Libode, Eastern Cape
- Authors: Zide, Lumka
- Date: 2024-04
- Subjects: Indigenous peoples -- Sports , Indigenous peoples -- Social life and customs , Minorities in sports
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63595 , vital:73506
- Description: This research focused on the Youth Participation in Sports: Reconsidering the Lost Culture of Indigenous Games in Libode, Eastern Cape. The study aimed to investigate the challenges facing indigenous games in Libode; to contribute within the anthropology of sport with specific reference to Libode in the Eastern Cape Province and to come up with recommendations with the view of addressing some of the challenges facing indigenous games in Libode. The study employed a qualitative approach and used interviews, questionnaires, observations, and participant observations as data collection methods. Data analysis was done through thematic data analysis. Cultural preservation was used as a conceptual framework for this study to understand the view of the participants in Libode. Furthermore, the literature that was reviewed in this research was based particularly on indigenous games to provide in-depth understanding the issues of indigenous games extinction as well to critique other authors who have written about indigenous games and their challenges. , Thesis (MA) -- Faculty of Humanities, School of Governmental and Social Sciences, 2024
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- Date Issued: 2024-04
- Authors: Zide, Lumka
- Date: 2024-04
- Subjects: Indigenous peoples -- Sports , Indigenous peoples -- Social life and customs , Minorities in sports
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63595 , vital:73506
- Description: This research focused on the Youth Participation in Sports: Reconsidering the Lost Culture of Indigenous Games in Libode, Eastern Cape. The study aimed to investigate the challenges facing indigenous games in Libode; to contribute within the anthropology of sport with specific reference to Libode in the Eastern Cape Province and to come up with recommendations with the view of addressing some of the challenges facing indigenous games in Libode. The study employed a qualitative approach and used interviews, questionnaires, observations, and participant observations as data collection methods. Data analysis was done through thematic data analysis. Cultural preservation was used as a conceptual framework for this study to understand the view of the participants in Libode. Furthermore, the literature that was reviewed in this research was based particularly on indigenous games to provide in-depth understanding the issues of indigenous games extinction as well to critique other authors who have written about indigenous games and their challenges. , Thesis (MA) -- Faculty of Humanities, School of Governmental and Social Sciences, 2024
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- Date Issued: 2024-04
Exploring the social construction of masculine identity as it relates to rape in the Buffalo City Municipality
- Matshikwe, Noluthando https://orcid.org/0000-0003-0439-0766
- Authors: Matshikwe, Noluthando https://orcid.org/0000-0003-0439-0766
- Date: 2024-01
- Subjects: Masculinity , Gender identity , Sex role
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/28595 , vital:74454
- Description: In South Africa, rape is a pandemic that is persistent and pervasive in unprecedented amounts. The construction of masculinity is understood to be a contributing factor to the rape pandemic in the country, as its construction focuses on power, dominance, and control of the less masculine individual. This study explored the social construction of masculine identity related to rape in the Buffalo City Municipality. The study sample consisted of a mixed-gendered focus group interview of eight participants (four males and four females). In addition, the study utilised six individual virtual interviews (three males and three females) who were all students at a local university. When analysing the data received from the participants, the study adopted Willig’s six stages of Foucauldian discourse analysis outlined in her book “Introducing Qualitative Research in Psychology” (2008, p. 118). The Foucauldian discourse analysis utilised in this study allowed for answering research questions through direct analysis of the extracts selected for this study. The results of this study indicated that masculinity is a socially constructed phenomenon synonymous with dominance and power. Hegemonic masculinity was constructed as synonymous with manhood, resulting in an individual being entitled to social privileges. Subordinate subjects were constructed as vulnerable to the expression of these qualities by being dominated, controlled, and raped. This construction of masculinity continues to construct robust and powerless subjects, where the powerless subject is subjected to the hegemonic male power, dominance, and control, creating an imbalance of social power and an unjust social hierarchy of. The construction of masculinity is linked with the rape pandemic faced by the country, as it constructs hegemonic forms of masculinity associated with domination and violation of other members of society, through sexual assault. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2024
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- Date Issued: 2024-01
- Authors: Matshikwe, Noluthando https://orcid.org/0000-0003-0439-0766
- Date: 2024-01
- Subjects: Masculinity , Gender identity , Sex role
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/28595 , vital:74454
- Description: In South Africa, rape is a pandemic that is persistent and pervasive in unprecedented amounts. The construction of masculinity is understood to be a contributing factor to the rape pandemic in the country, as its construction focuses on power, dominance, and control of the less masculine individual. This study explored the social construction of masculine identity related to rape in the Buffalo City Municipality. The study sample consisted of a mixed-gendered focus group interview of eight participants (four males and four females). In addition, the study utilised six individual virtual interviews (three males and three females) who were all students at a local university. When analysing the data received from the participants, the study adopted Willig’s six stages of Foucauldian discourse analysis outlined in her book “Introducing Qualitative Research in Psychology” (2008, p. 118). The Foucauldian discourse analysis utilised in this study allowed for answering research questions through direct analysis of the extracts selected for this study. The results of this study indicated that masculinity is a socially constructed phenomenon synonymous with dominance and power. Hegemonic masculinity was constructed as synonymous with manhood, resulting in an individual being entitled to social privileges. Subordinate subjects were constructed as vulnerable to the expression of these qualities by being dominated, controlled, and raped. This construction of masculinity continues to construct robust and powerless subjects, where the powerless subject is subjected to the hegemonic male power, dominance, and control, creating an imbalance of social power and an unjust social hierarchy of. The construction of masculinity is linked with the rape pandemic faced by the country, as it constructs hegemonic forms of masculinity associated with domination and violation of other members of society, through sexual assault. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2024
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- Date Issued: 2024-01
'N polisistemiese ondersoek na die poësie van instagram-digters
- Authors: De Wat Jane
- Date: 2023-12
- Subjects: Prosodic analysis (Linguistics) , Poetry
- Language: Afrikaans
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63265 , vital:73242
- Description: Die doel van hierdie studie is om die opkoms van Afrikaanse Instagrampoësie en die literêre en sosiale status daarvan te ondersoek met spesifieke verwysing na die volgende Afrikaanse aanlyn digters en hul selfpublikasies: Skakerings van geel (@skildermetdigkuns) en Alles wat ek wou sê (@ontmasker), sowel as die sogenaamde Rupi Kaur van Afrikaanse Instagrampoësie, (@kaalwoorde), se digbundel Kosmos en komete wat onlangs deur LAPA Uitgewers gepubliseer is. Daar word ondersoek ingestel na die manier waarop die metamodernistiese konteks waarbinne digitale of e-letterkunde ontwikkel het, in dié skryfstyl manifesteer. In my ondersoek fokus ek op metamodernisme eerder as postmodernisme, omdat metamodernisme groter klem op die waarheid en die objektiewe realiteit plaas, wat ʼn opvallende tema in die werk van die gekose digters is. Die impak van aanlyn poësie op die identiteit en voorstelling van die Zoomer-generasie (individue wat ná 1996 gebore is, andersins bekend as generasie Z) word nagespeur ten einde die impak van hierdie poësie op die gemiddelde Afrikaanse Zoomer-leser te bepaal. Verskeie vrae rakende die posisie van Instagrampoësie binne die Afrikaanse literêre diskoers en kanon word ook onder die loep geneem. Die analise en interpretasie van die gedigte is daarop gemik om die literêre status van hierdie gedigte te ondersoek op grond van skryfpatrone, die evolusie daarvan en die gewildheid wat gemeet word in terme van followers (voortaan volgelinge), likes (voortaan bekend as voorkeure) en comments (voortaan kommentaar).1 Daar is bewys dat die metamodernistiese konteks (wat breedvoerig in die studie gekonseptualiseer en in terme van Suid-Afrika gekontekstualiseer word) waarin hierdie gedigte geformuleer en versprei is, ʼn beduidende invloed het op die toon en temas daarvan. Daar word getoon dat die tematiese stryd met kwessies soos identiteit, geloof en liefde in dié woordkuns verband hou met die sosiopolitieke konteks van die Zoomer-generasie. Hierdie metamodernistiese toestand word gekenmerk deur progressiewe denke, diversiteit en ʼn samelewing wat voortdurend verander. Die gevolgtrekking word gemaak dat Afrikaanse Instagrampoësie ʼn literêre diskoers skep met die doel om nuwe idees met betrekking tot die waarhede van ʼn generasie wat dikwels misverstaan en misgekyk word, voor te stel. Die vryheid van Instagrampoësie bied met ander woorde ʼn geleentheid vir Zoomer-denkers om hulle stempel binne ʼn metamoderne samelewing af te druk. Deur Even-Zohar se 1 Instagram-terme wat verwys na die hoeveelheid mense wat die digter volg, sy/haar/sig gedigte lees en dan ook daarop reageer op ʼn “hou van/hou nie van nie” glyskaal. 4 polisisteemteorie en die leser-reaksie-teorie by die ondersoek na die skep en ontvangs van aanlyn poësie te betrek, word aangetoon dat e-letterkunde wel ʼn plek binne die Afrikaanse literêre diskoers vind. Hoewel die skeppingsproses, publikasie en ontvangs van aanlyn poësie verskil van dié van ander literêre vorme, stel die studie hierdie vorm van poësie aan die Afrikaanse literêre diskoers en kanon bekend. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: De Wat Jane
- Date: 2023-12
- Subjects: Prosodic analysis (Linguistics) , Poetry
- Language: Afrikaans
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63265 , vital:73242
- Description: Die doel van hierdie studie is om die opkoms van Afrikaanse Instagrampoësie en die literêre en sosiale status daarvan te ondersoek met spesifieke verwysing na die volgende Afrikaanse aanlyn digters en hul selfpublikasies: Skakerings van geel (@skildermetdigkuns) en Alles wat ek wou sê (@ontmasker), sowel as die sogenaamde Rupi Kaur van Afrikaanse Instagrampoësie, (@kaalwoorde), se digbundel Kosmos en komete wat onlangs deur LAPA Uitgewers gepubliseer is. Daar word ondersoek ingestel na die manier waarop die metamodernistiese konteks waarbinne digitale of e-letterkunde ontwikkel het, in dié skryfstyl manifesteer. In my ondersoek fokus ek op metamodernisme eerder as postmodernisme, omdat metamodernisme groter klem op die waarheid en die objektiewe realiteit plaas, wat ʼn opvallende tema in die werk van die gekose digters is. Die impak van aanlyn poësie op die identiteit en voorstelling van die Zoomer-generasie (individue wat ná 1996 gebore is, andersins bekend as generasie Z) word nagespeur ten einde die impak van hierdie poësie op die gemiddelde Afrikaanse Zoomer-leser te bepaal. Verskeie vrae rakende die posisie van Instagrampoësie binne die Afrikaanse literêre diskoers en kanon word ook onder die loep geneem. Die analise en interpretasie van die gedigte is daarop gemik om die literêre status van hierdie gedigte te ondersoek op grond van skryfpatrone, die evolusie daarvan en die gewildheid wat gemeet word in terme van followers (voortaan volgelinge), likes (voortaan bekend as voorkeure) en comments (voortaan kommentaar).1 Daar is bewys dat die metamodernistiese konteks (wat breedvoerig in die studie gekonseptualiseer en in terme van Suid-Afrika gekontekstualiseer word) waarin hierdie gedigte geformuleer en versprei is, ʼn beduidende invloed het op die toon en temas daarvan. Daar word getoon dat die tematiese stryd met kwessies soos identiteit, geloof en liefde in dié woordkuns verband hou met die sosiopolitieke konteks van die Zoomer-generasie. Hierdie metamodernistiese toestand word gekenmerk deur progressiewe denke, diversiteit en ʼn samelewing wat voortdurend verander. Die gevolgtrekking word gemaak dat Afrikaanse Instagrampoësie ʼn literêre diskoers skep met die doel om nuwe idees met betrekking tot die waarhede van ʼn generasie wat dikwels misverstaan en misgekyk word, voor te stel. Die vryheid van Instagrampoësie bied met ander woorde ʼn geleentheid vir Zoomer-denkers om hulle stempel binne ʼn metamoderne samelewing af te druk. Deur Even-Zohar se 1 Instagram-terme wat verwys na die hoeveelheid mense wat die digter volg, sy/haar/sig gedigte lees en dan ook daarop reageer op ʼn “hou van/hou nie van nie” glyskaal. 4 polisisteemteorie en die leser-reaksie-teorie by die ondersoek na die skep en ontvangs van aanlyn poësie te betrek, word aangetoon dat e-letterkunde wel ʼn plek binne die Afrikaanse literêre diskoers vind. Hoewel die skeppingsproses, publikasie en ontvangs van aanlyn poësie verskil van dié van ander literêre vorme, stel die studie hierdie vorm van poësie aan die Afrikaanse literêre diskoers en kanon bekend. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
A case study of how to implement continuous improvement initiatives
- Authors: Ntsoane, Makobe Collen
- Date: 2023-12
- Subjects: Employee morale , Incentives in industry , Corporate culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/65902 , vital:74282
- Description: The aim of this study was to establish an understanding of the critical success factors of how to successfully implement and sustain continuous improvement projects at the Festive poultry processing plant using the 20 Keys continuous improvement (CI) tool. There are many continuous improvements in methodologies such as lean, 20 Keys , TQM and business process re-engineering. However, it has proven difficult for numerous businesses globally. Festive, a division of Astral Foods, a poultry processing plant, attempted to implement the 20 Keys , a continuous improvement methodology, and it was no exception as it was added to the list of unsuccessful companies. Because of the failed attempt to implement 20 Keys at Festive and many other manufacturing businesses, which attempted to introduce CI initiatives but ultimately failed, factors such as management support, training, rewards and recognition and organisational culture have been revealed by the literature review as contributors to continuous improvement initiatives’ successful or unsuccessful implementation. A case study methodology was applied in this study to understand the real-life context of the failed implementation of Continuous Improvement at Festive. Furthermore, it aimed to explore and explain complex causal links of training, rewards and recognition, organisational culture and management support which can positively impact the implementation of 20 Keys within the poultry processing industry or any manufacturing industry. A mixed method was followed through an online survey and in-depth interviews. The qualitative and quantitative data analysis results revealed that management support, training, rewards and recognition and organisational culture all were related and influenced continuous improvement implementation. Furthermore, companywide training, rewards and recognition proved to be the most significant challenge, resulting in the unsuccessful implementation of 20 Keys. The findings revealed that implementing continuous improvement is not immediate and entails all employees being adequately involved in the entire process from the start. The main finding at Festive was that when implementing the 20 Keys continuous , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business School, 2023 , The aim of this study was to establish an understanding of the critical success factors of how to successfully implement and sustain continuous improvement projects at the Festive poultry processing plant using the 20 Keys continuous improvement (CI) tool. There are many continuous improvements in methodologies such as lean, 20 Keys , TQM and business process re-engineering. However, it has proven difficult for numerous businesses globally. Festive, a division of Astral Foods, a poultry processing plant, attempted to implement the 20 Keys , a continuous improvement methodology, and it was no exception as it was added to the list of unsuccessful companies. Because of the failed attempt to implement 20 Keys at Festive and many other manufacturing businesses, which attempted to introduce CI initiatives but ultimately failed, factors such as management support, training, rewards and recognition and organisational culture have been revealed by the literature review as contributors to continuous improvement initiatives’ successful or unsuccessful implementation. A case study methodology was applied in this study to understand the real-life context of the failed implementation of Continuous Improvement at Festive. Furthermore, it aimed to explore and explain complex causal links of training, rewards and recognition, organisational culture and management support which can positively impact the implementation of 20 Keys within the poultry processing industry or any manufacturing industry. A mixed method was followed through an online survey and in-depth interviews. The qualitative and quantitative data analysis results revealed that management support, training, rewards and recognition and organisational culture all were related and influenced continuous improvement implementation. Furthermore, companywide training, rewards and recognition proved to be the most significant challenge, resulting in the unsuccessful implementation of 20 Keys. The findings revealed that implementing continuous improvement is not immediate and entails all employees being adequately involved in the entire process from the start. The main finding at Festive was that when implementing the 20 Keys continuous improvement tool, the training provided was inadequate and mainly focused on managers and not staff. Furthermore, the lack of a rewards and recognition strategy, linked to continuous improvement goals, contributed to resistance to change in the culture and an inability to adapt to change. Misalignment between managers and staff regarding what contributes to successful continuous improvement initiatives, such as training, management involvement and rewards and recognition, was also found to be a contributor to the unsuccessful implementation of continuous improvement.
- Full Text:
- Date Issued: 2023-12
- Authors: Ntsoane, Makobe Collen
- Date: 2023-12
- Subjects: Employee morale , Incentives in industry , Corporate culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/65902 , vital:74282
- Description: The aim of this study was to establish an understanding of the critical success factors of how to successfully implement and sustain continuous improvement projects at the Festive poultry processing plant using the 20 Keys continuous improvement (CI) tool. There are many continuous improvements in methodologies such as lean, 20 Keys , TQM and business process re-engineering. However, it has proven difficult for numerous businesses globally. Festive, a division of Astral Foods, a poultry processing plant, attempted to implement the 20 Keys , a continuous improvement methodology, and it was no exception as it was added to the list of unsuccessful companies. Because of the failed attempt to implement 20 Keys at Festive and many other manufacturing businesses, which attempted to introduce CI initiatives but ultimately failed, factors such as management support, training, rewards and recognition and organisational culture have been revealed by the literature review as contributors to continuous improvement initiatives’ successful or unsuccessful implementation. A case study methodology was applied in this study to understand the real-life context of the failed implementation of Continuous Improvement at Festive. Furthermore, it aimed to explore and explain complex causal links of training, rewards and recognition, organisational culture and management support which can positively impact the implementation of 20 Keys within the poultry processing industry or any manufacturing industry. A mixed method was followed through an online survey and in-depth interviews. The qualitative and quantitative data analysis results revealed that management support, training, rewards and recognition and organisational culture all were related and influenced continuous improvement implementation. Furthermore, companywide training, rewards and recognition proved to be the most significant challenge, resulting in the unsuccessful implementation of 20 Keys. The findings revealed that implementing continuous improvement is not immediate and entails all employees being adequately involved in the entire process from the start. The main finding at Festive was that when implementing the 20 Keys continuous , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business School, 2023 , The aim of this study was to establish an understanding of the critical success factors of how to successfully implement and sustain continuous improvement projects at the Festive poultry processing plant using the 20 Keys continuous improvement (CI) tool. There are many continuous improvements in methodologies such as lean, 20 Keys , TQM and business process re-engineering. However, it has proven difficult for numerous businesses globally. Festive, a division of Astral Foods, a poultry processing plant, attempted to implement the 20 Keys , a continuous improvement methodology, and it was no exception as it was added to the list of unsuccessful companies. Because of the failed attempt to implement 20 Keys at Festive and many other manufacturing businesses, which attempted to introduce CI initiatives but ultimately failed, factors such as management support, training, rewards and recognition and organisational culture have been revealed by the literature review as contributors to continuous improvement initiatives’ successful or unsuccessful implementation. A case study methodology was applied in this study to understand the real-life context of the failed implementation of Continuous Improvement at Festive. Furthermore, it aimed to explore and explain complex causal links of training, rewards and recognition, organisational culture and management support which can positively impact the implementation of 20 Keys within the poultry processing industry or any manufacturing industry. A mixed method was followed through an online survey and in-depth interviews. The qualitative and quantitative data analysis results revealed that management support, training, rewards and recognition and organisational culture all were related and influenced continuous improvement implementation. Furthermore, companywide training, rewards and recognition proved to be the most significant challenge, resulting in the unsuccessful implementation of 20 Keys. The findings revealed that implementing continuous improvement is not immediate and entails all employees being adequately involved in the entire process from the start. The main finding at Festive was that when implementing the 20 Keys continuous improvement tool, the training provided was inadequate and mainly focused on managers and not staff. Furthermore, the lack of a rewards and recognition strategy, linked to continuous improvement goals, contributed to resistance to change in the culture and an inability to adapt to change. Misalignment between managers and staff regarding what contributes to successful continuous improvement initiatives, such as training, management involvement and rewards and recognition, was also found to be a contributor to the unsuccessful implementation of continuous improvement.
- Full Text:
- Date Issued: 2023-12
A Combined Experimental and Computational Study of Hydridospirophosphorane Ligand Systems Featuring Halogenated Mandelic Acids
- Authors: Maritz, Marius Johann
- Date: 2023-12
- Subjects: Organic compounds -- Synthesis , Heterocyclic compounds , catalysis -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62096 , vital:71911
- Description: The search for new stereoselective catalysts remains important for the isolation of enantiomeric products from racemic mixtures. The need exists for these catalysts to be more efficient, to be more cost effective as well as be stable over time without undergoing changes in molecular structure and selectivity. The purpose of this research project was to experimentally synthesise and characterise asymmetric catalysts for the use in enantioselective synthesis. The catalysts consisted of the main group element phosphorous as central atom, with the phenyl rings of mandelic acid ligands monosubstituted with halogen atoms fluorine, chlorine and bromine in different configurations. Ligand binding resulted in the formation of hydridospirophosphorane structures, from which the molecular properties and binding geometry of these molecules could be explained by the theory of apicophilicity. Characterization was performed by NMR and IR spectroscopy as well as diffraction studies that provided the experimental crystal structures. The structural, energetic and spectroscopy results were compared to the theoretically obtained molecular properties using DFT analysis. Various interand intramolecular interactions that existed between molecules found in crystal packing environments were discussed. Additional properties that were investigated included modelling solvation effects, molecular orbital analysis, Hirshfeld surfaces, orbital and atomic energy and population analysis as well as ESP energy calculations with the optimized crystal structures as input. Molecular overlay comparisons were also performed between the experimental and optimized structures where the effectiveness of various DFT functionals and basis sets could be determined. The method with the best overall cost-to-accuracy ratio was found to be the triple-zeta def2-tzvp basis set with B3LYP functional theory and the addition of Grimme’s dispersion correction. Results indicated differences in crystal packing depending largely on the given halogen atoms present in the substituted phenyl rings, with differences observed in electronegativity and steric effects. One of the crystal systems showed additional interactions with solvent molecules, giving the impression that obtaining crystal void formation was possible. Alterations in the transition state activation energies between the isomers of each molecule were found to be present and supported the theory behind the mechanism of stereochemical induction. The studied compounds were therefore effective in isolating different enantiomeric ligands by means of energy differences between conformers and displayed unique catalytic properties resulting from the phosphorous main group element. The hydridospirophosphoranes conformers responsible for the lowest theoretically calculated activation energy induced crystallization as was seen by diffraction results for all compounds. Even as an intriguing observation, crystallization will largely depend on solubility rather than a specific conformer’s amount in solution. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Maritz, Marius Johann
- Date: 2023-12
- Subjects: Organic compounds -- Synthesis , Heterocyclic compounds , catalysis -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62096 , vital:71911
- Description: The search for new stereoselective catalysts remains important for the isolation of enantiomeric products from racemic mixtures. The need exists for these catalysts to be more efficient, to be more cost effective as well as be stable over time without undergoing changes in molecular structure and selectivity. The purpose of this research project was to experimentally synthesise and characterise asymmetric catalysts for the use in enantioselective synthesis. The catalysts consisted of the main group element phosphorous as central atom, with the phenyl rings of mandelic acid ligands monosubstituted with halogen atoms fluorine, chlorine and bromine in different configurations. Ligand binding resulted in the formation of hydridospirophosphorane structures, from which the molecular properties and binding geometry of these molecules could be explained by the theory of apicophilicity. Characterization was performed by NMR and IR spectroscopy as well as diffraction studies that provided the experimental crystal structures. The structural, energetic and spectroscopy results were compared to the theoretically obtained molecular properties using DFT analysis. Various interand intramolecular interactions that existed between molecules found in crystal packing environments were discussed. Additional properties that were investigated included modelling solvation effects, molecular orbital analysis, Hirshfeld surfaces, orbital and atomic energy and population analysis as well as ESP energy calculations with the optimized crystal structures as input. Molecular overlay comparisons were also performed between the experimental and optimized structures where the effectiveness of various DFT functionals and basis sets could be determined. The method with the best overall cost-to-accuracy ratio was found to be the triple-zeta def2-tzvp basis set with B3LYP functional theory and the addition of Grimme’s dispersion correction. Results indicated differences in crystal packing depending largely on the given halogen atoms present in the substituted phenyl rings, with differences observed in electronegativity and steric effects. One of the crystal systems showed additional interactions with solvent molecules, giving the impression that obtaining crystal void formation was possible. Alterations in the transition state activation energies between the isomers of each molecule were found to be present and supported the theory behind the mechanism of stereochemical induction. The studied compounds were therefore effective in isolating different enantiomeric ligands by means of energy differences between conformers and displayed unique catalytic properties resulting from the phosphorous main group element. The hydridospirophosphoranes conformers responsible for the lowest theoretically calculated activation energy induced crystallization as was seen by diffraction results for all compounds. Even as an intriguing observation, crystallization will largely depend on solubility rather than a specific conformer’s amount in solution. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
- Full Text:
- Date Issued: 2023-12
A qualitative review on the manner in which psychologists address language barriers in psychological testing with isiXhosa speaking clients.
- Authors: Fouche, Nicoleen
- Date: 2023-12
- Subjects: language barriers , isiXhosa , psychological tests,
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62838 , vital:73055
- Description: A racially segregated culture historically influenced the growth of psychological testing and evaluation in South Africa. Thus, South Africa's multicultural and multilingual community poses difficulties for psychologists in their daily work. Most of the psychological tests used by psychologists are predominantly normed in either or only English/Afrikaans. Given the economic and professional resources required to develop new standardised tests for the broader South African population, the current status quo is set to continue for the foreseeable future. Consequently, language barriers pose a challenge when psychometric standardised tests are administered in a language that is different from that of the client, and as a result, psychologists must develop ways to enhance the fairness of these measures for their client base. The avenue of research that was explored is how psychologists overcome the language boundary when assessing clients who speak an indigenous first language, particularly isiXhosa. As such, this study explored this question through qualitative methods. Through purposive sampling, nine psychologists were invited to share their practical expertise on this topic, using semi-structured online interviews. The findings of this study suggested several core themes providing useful methods for other psychologists facing the same challenge, including the challenges with administering psychological tests with isiXhosa clients, the availability of standardised tests in the South African context, and the importance of determining the client’s language proficiency. Five themes with several sub-themes emerged from the study. The study contributes to the currently limited existing body of knowledge, and subsequently to the research area that this study explores - thus further providing guidelines that practitioners can employ when working with clients who are isiXhosa speaking. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Fouche, Nicoleen
- Date: 2023-12
- Subjects: language barriers , isiXhosa , psychological tests,
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62838 , vital:73055
- Description: A racially segregated culture historically influenced the growth of psychological testing and evaluation in South Africa. Thus, South Africa's multicultural and multilingual community poses difficulties for psychologists in their daily work. Most of the psychological tests used by psychologists are predominantly normed in either or only English/Afrikaans. Given the economic and professional resources required to develop new standardised tests for the broader South African population, the current status quo is set to continue for the foreseeable future. Consequently, language barriers pose a challenge when psychometric standardised tests are administered in a language that is different from that of the client, and as a result, psychologists must develop ways to enhance the fairness of these measures for their client base. The avenue of research that was explored is how psychologists overcome the language boundary when assessing clients who speak an indigenous first language, particularly isiXhosa. As such, this study explored this question through qualitative methods. Through purposive sampling, nine psychologists were invited to share their practical expertise on this topic, using semi-structured online interviews. The findings of this study suggested several core themes providing useful methods for other psychologists facing the same challenge, including the challenges with administering psychological tests with isiXhosa clients, the availability of standardised tests in the South African context, and the importance of determining the client’s language proficiency. Five themes with several sub-themes emerged from the study. The study contributes to the currently limited existing body of knowledge, and subsequently to the research area that this study explores - thus further providing guidelines that practitioners can employ when working with clients who are isiXhosa speaking. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
A study exploring the understanding and interpretations of schizophrenia amongst Zulu Community members, in Durban, Kwa-Zulu Natal Province
- Authors: Lephoto, Thabo
- Date: 2023-12
- Subjects: chizophrenia , traditional healers , cultural belief,
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62859 , vital:73059
- Description: Mental illness is a global and widespread phenomenon, and its presented symptoms are experienced differently within various contexts. In many contexts, cultural beliefs are noted as influencing the thinking, feeling, and behavior of individuals and groups. Schizophrenia is often associated with a clustering of symptoms, which are often interpreted from within a cultural lens. This is in part, due to the lack of access to mental health facilities, and the limited understanding of psychological processes, but primarily due to cultural belief systems. As such, many individuals rely on indigenous knowledge systems and traditional healing when experiencing psychological symptoms. Within South Africa, the symptoms associated with schizophrenia are understood and treated in accordance with cultural beliefs and it is likely that bewitchment, spirit possession, as well as ancestral callings such as ukuthwasa, etc. will be associated with the symptoms of mental illnesses. The implications that this poses to psychological treatments and interventions are vast and require exploration. This study employed a qualitative methodology, with an exploratory and descriptive design. There were 8 participants (males= 4; females= 4), aged between 26-37 that were recruited for the study by utilizing a purposive sampling strategy, as well as a snowball sampling technique. Thematic analysis was used as a means of data analysis, by following the method by Braun and Clarke. The study applies the principles of ethics with regard to confidentiality and privacy. The findings of the study revealed that participants’ perceptions of schizophrenia were influenced by their culture. Participants of the study suggested that culture is influential in the treatment of those diagnosed with schizophrenia. Furthermore, the study revealed that there is a hierarchical system in the Zulu Culture and those diagnosed with schizophrenia are placed at the bottom. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Lephoto, Thabo
- Date: 2023-12
- Subjects: chizophrenia , traditional healers , cultural belief,
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62859 , vital:73059
- Description: Mental illness is a global and widespread phenomenon, and its presented symptoms are experienced differently within various contexts. In many contexts, cultural beliefs are noted as influencing the thinking, feeling, and behavior of individuals and groups. Schizophrenia is often associated with a clustering of symptoms, which are often interpreted from within a cultural lens. This is in part, due to the lack of access to mental health facilities, and the limited understanding of psychological processes, but primarily due to cultural belief systems. As such, many individuals rely on indigenous knowledge systems and traditional healing when experiencing psychological symptoms. Within South Africa, the symptoms associated with schizophrenia are understood and treated in accordance with cultural beliefs and it is likely that bewitchment, spirit possession, as well as ancestral callings such as ukuthwasa, etc. will be associated with the symptoms of mental illnesses. The implications that this poses to psychological treatments and interventions are vast and require exploration. This study employed a qualitative methodology, with an exploratory and descriptive design. There were 8 participants (males= 4; females= 4), aged between 26-37 that were recruited for the study by utilizing a purposive sampling strategy, as well as a snowball sampling technique. Thematic analysis was used as a means of data analysis, by following the method by Braun and Clarke. The study applies the principles of ethics with regard to confidentiality and privacy. The findings of the study revealed that participants’ perceptions of schizophrenia were influenced by their culture. Participants of the study suggested that culture is influential in the treatment of those diagnosed with schizophrenia. Furthermore, the study revealed that there is a hierarchical system in the Zulu Culture and those diagnosed with schizophrenia are placed at the bottom. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
Adherence narratives of COVID-19 in KwaNobuhle Township, Eastern Cape
- Authors: Andries, Siyanda
- Date: 2023-12
- Subjects: Coronavirus Disease--Eastern Cape--South Africa , Vaccination--Eastern Cape, South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63243 , vital:73240
- Description: The emergence of COVID-19 triggered a global health crisis that not only took many lives but disrupted economies, strained healthcare systems, and impacted social relations globally. KwaNobuhle township in the Eastern Cape province of South Africa falls under the Nelson Mandela Bay Metropolitan Municipality. This municipality experienced some of South Africa’s highest COVID-19 fatalities, with the lowest levels of testing, active cases and hospitalisation in the country. This study investigates the narratives about COVID-19 prevention measures in KwaNobuhle. Primarily, it seeks to reveal how young and elderly peoples’ perceptions about COVID-19 influenced their compliance with prevention measures such as lockdown, testing and self-admission to hospital when sick. Fifteen residents of KwaNobuhle, who identify as Black people between 18 and 30 years and 50 and 65 years, were recruited as participants in this study. The two groups of study participants were recruited in order to enable an investigation into intergenerational narratives about infectious disease. In this study, Intergenerational narratives encompass stories, beliefs, and cultural traditions that transmit values, historical experiences, and a shared sense of identity, fostering connections between generations. This study provides an historical overview of how communities dealt with previous epidemics in South Africa and analyses how COVID-19 disrupted the lives of people with the preventative measures and changes that were implemented by the government. Social constructivism is a theory that emphasizes the role of social interactions and cultural context in shaping individuals' understanding of the world. Social constructivism theory is employed to frame the investigation of narratives of COVID-19 and is based on qualitative data collected through in-depth telephonic individual interviews. The data reveals that participants’ perceptions and narratives of diseases are shaped and influenced by their experiences and knowledge of the same. The findings confirm that the two different age groups of participants have distinct perspectives of COVID-19. Older participants rely on their past experiences of diseases, while younger participants form their understanding through mainstream and social media. This study further examines the inadequacy of healthcare system preparedness in South Africa to adequately address the onset of infectious diseases. Furthermore, this investigation unveils the way preventive measures against COVID-19 imposed inconvenience upon individuals' daily lives and overall lifestyles. These measures included curfews, a limitation on crowd size in public spaces vii such as markets, and the prohibition of alcohol consumption. Individuals who were reliant on the alcohol industry as their primary source of income were adversely affected. African traditional practices were also impacted, as burial custom alterations resulted. Lastly, the study illuminates that the acceptance and uptake of vaccinations was influenced by vaccine hesitancy, with social media serving as a prominent conduit for the proliferation of conspiracy theories in the context of COVID-19. , Thesis (Ma) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Andries, Siyanda
- Date: 2023-12
- Subjects: Coronavirus Disease--Eastern Cape--South Africa , Vaccination--Eastern Cape, South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63243 , vital:73240
- Description: The emergence of COVID-19 triggered a global health crisis that not only took many lives but disrupted economies, strained healthcare systems, and impacted social relations globally. KwaNobuhle township in the Eastern Cape province of South Africa falls under the Nelson Mandela Bay Metropolitan Municipality. This municipality experienced some of South Africa’s highest COVID-19 fatalities, with the lowest levels of testing, active cases and hospitalisation in the country. This study investigates the narratives about COVID-19 prevention measures in KwaNobuhle. Primarily, it seeks to reveal how young and elderly peoples’ perceptions about COVID-19 influenced their compliance with prevention measures such as lockdown, testing and self-admission to hospital when sick. Fifteen residents of KwaNobuhle, who identify as Black people between 18 and 30 years and 50 and 65 years, were recruited as participants in this study. The two groups of study participants were recruited in order to enable an investigation into intergenerational narratives about infectious disease. In this study, Intergenerational narratives encompass stories, beliefs, and cultural traditions that transmit values, historical experiences, and a shared sense of identity, fostering connections between generations. This study provides an historical overview of how communities dealt with previous epidemics in South Africa and analyses how COVID-19 disrupted the lives of people with the preventative measures and changes that were implemented by the government. Social constructivism is a theory that emphasizes the role of social interactions and cultural context in shaping individuals' understanding of the world. Social constructivism theory is employed to frame the investigation of narratives of COVID-19 and is based on qualitative data collected through in-depth telephonic individual interviews. The data reveals that participants’ perceptions and narratives of diseases are shaped and influenced by their experiences and knowledge of the same. The findings confirm that the two different age groups of participants have distinct perspectives of COVID-19. Older participants rely on their past experiences of diseases, while younger participants form their understanding through mainstream and social media. This study further examines the inadequacy of healthcare system preparedness in South Africa to adequately address the onset of infectious diseases. Furthermore, this investigation unveils the way preventive measures against COVID-19 imposed inconvenience upon individuals' daily lives and overall lifestyles. These measures included curfews, a limitation on crowd size in public spaces vii such as markets, and the prohibition of alcohol consumption. Individuals who were reliant on the alcohol industry as their primary source of income were adversely affected. African traditional practices were also impacted, as burial custom alterations resulted. Lastly, the study illuminates that the acceptance and uptake of vaccinations was influenced by vaccine hesitancy, with social media serving as a prominent conduit for the proliferation of conspiracy theories in the context of COVID-19. , Thesis (Ma) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
An architecture for feedback-driven learning analytics
- Authors: Winfield, Philip John
- Date: 2023-12
- Subjects: Educational statistics -- Data processing , Education -- Research -- Statistical methods , Teacher-student relationships
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62744 , vital:72935
- Description: Feedback from students provides an opportunity to gain insights into students’ learning behaviour and participation in higher education learning and teaching. Academic staff are expected to constantly review and improve the learning and teaching environment where feedback contributes vital information toward pedagogical decision-making. Reviewing student feedback is essential but time-consuming, making it crucial to explore more effective and efficient ways to analyse and present feedback that encourages intervention and support. The general design science research evaluation pattern guided the construction and evaluation of a Feedback-Driven Architecture (FDA) for Learning Analytics (LA) to address this problem. An FDA implementation using a suitable case demonstrated each component validating the feasibility and effectiveness of the proposed design. The components identified for inclusion in the FDA were integrated within layers of a three-tiered architecture pattern. The data layer incorporates the collection, preparation and storage of learning management system data which includes free-form narrative student feedback. The application layer contains logic to support the analysis of free-form narrative student feedback and extraction of learning analytics. Arrangement and visualisation of information in the presentation layer aim to promote appropriate intervention and support. Evaluation using a case study showed that the FDA provided necessary guidelines for developing an implementation that produced feedbackdriven learning analytics. Data preparation transformed unstructured data into a suitable representation for effective analysis. Cohorts within narrative feedback responses were identified using the k-means clustering algorithm and latent Dirichlet allocation. Logistic regression and support vector machines were applied as predictive models and trained using extracted quantitative markers to predict academic success. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Winfield, Philip John
- Date: 2023-12
- Subjects: Educational statistics -- Data processing , Education -- Research -- Statistical methods , Teacher-student relationships
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62744 , vital:72935
- Description: Feedback from students provides an opportunity to gain insights into students’ learning behaviour and participation in higher education learning and teaching. Academic staff are expected to constantly review and improve the learning and teaching environment where feedback contributes vital information toward pedagogical decision-making. Reviewing student feedback is essential but time-consuming, making it crucial to explore more effective and efficient ways to analyse and present feedback that encourages intervention and support. The general design science research evaluation pattern guided the construction and evaluation of a Feedback-Driven Architecture (FDA) for Learning Analytics (LA) to address this problem. An FDA implementation using a suitable case demonstrated each component validating the feasibility and effectiveness of the proposed design. The components identified for inclusion in the FDA were integrated within layers of a three-tiered architecture pattern. The data layer incorporates the collection, preparation and storage of learning management system data which includes free-form narrative student feedback. The application layer contains logic to support the analysis of free-form narrative student feedback and extraction of learning analytics. Arrangement and visualisation of information in the presentation layer aim to promote appropriate intervention and support. Evaluation using a case study showed that the FDA provided necessary guidelines for developing an implementation that produced feedbackdriven learning analytics. Data preparation transformed unstructured data into a suitable representation for effective analysis. Cohorts within narrative feedback responses were identified using the k-means clustering algorithm and latent Dirichlet allocation. Logistic regression and support vector machines were applied as predictive models and trained using extracted quantitative markers to predict academic success. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2023
- Full Text:
- Date Issued: 2023-12
Assessing land degradation and the effectiveness of calcrete bontveld rehabilitation in a grassridge PPC cement mining area using multi-sensor remotely sensed data and machine learning techniques
- Authors: Mpisane, Khanyisa
- Date: 2023-12
- Subjects: Land degradation -- South Africa , Environmental degradation , Mines and mineral resources -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62527 , vital:72821
- Description: This study uses multi-temporal remote sensing data to spatially visualise and quantify land degradation due to mining as well as Calcrete Bontveld rehabilitation at the Grassridge PPC Cement mine, Gqeberha formerly known as Port Elizabeth in the Eastern Cape, South Africa. Botanical field data is also used to verify the suasses of rehabilitation in the area. SPOT-6 and Landsat multi-spectral images were compared, and Support Vector Machine and Random Forest algorithms were used for classification in order to determine which yields more accurate results for a limestone mine. Support Vector Machine analysis using SPOT-6 images yielded the best results. This was due to the high spatial resolution of SPOT-6 compared to Landsat and Support Vector Machine classifier was able to classify images with fewer training points compared to Random Forest. The spatio-temporal land cover change at the mine was then determined between the years 2000, 2015 and 2020. Land cover classification is useful for monitoring land degradation and, in this case, was able to show the extent of rehabilitation success. For the year 2020, a 17% area was rehabilitated; however, the algorithm could not distinguish between unmined Calcrete Bontveld matrix and rehabilitation sites that were older than five years. The performed change detection also showed that 29.50% of unmined Calcrete Bontveld matrix had changed to “mature rehabilitation” (rehabilitation sites older than five years). Again, for this percentage in some areas the algorithm could not distinguish between the unmined Calcrete Bontveld matrix and rehabilitation sites that were older than five years due to high similarities between the two land cover types. Area changes of the different land cover classes could also be used to demonstrate how rehabilitation areas have matured over time and lead to the conclusion that most of the Calcrete Bontveld which was mined, has over the years been successfully rehabilitated. Vegetation analysis was conducted to further validate the rehabilitation success of Calcrete Bontveld matrix. Multivariant Detrended Correspondent Analysis showed that rehabilitation sites which were younger than five years (2–year-old rehabilitation plots that were sampled) had great dissimilarity to the natural unmined Calcrete Bontveld matrix and that rehabilitation sites older than five years, in this case 16–years older, had high similarity and resemblance to natural unmined Calcrete Bontveld matrix and therefore could be considered as being mature. This was a more definitive assessment as it considers all aspects of the vegetation. Species cover and species richness also showed that Calcrete Bontveld matrix rehabilitation sites which have been rehabilitated for more than 5 years had greater similarity to natural unmined vegetation compared to areas that have been rehabilitated for less than five years. This study, therefore, demonstrates that due to the high similarity between mature rehabilitation sites and unmined Calcrete Bontveld, rehabilitation has been successful. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Mpisane, Khanyisa
- Date: 2023-12
- Subjects: Land degradation -- South Africa , Environmental degradation , Mines and mineral resources -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62527 , vital:72821
- Description: This study uses multi-temporal remote sensing data to spatially visualise and quantify land degradation due to mining as well as Calcrete Bontveld rehabilitation at the Grassridge PPC Cement mine, Gqeberha formerly known as Port Elizabeth in the Eastern Cape, South Africa. Botanical field data is also used to verify the suasses of rehabilitation in the area. SPOT-6 and Landsat multi-spectral images were compared, and Support Vector Machine and Random Forest algorithms were used for classification in order to determine which yields more accurate results for a limestone mine. Support Vector Machine analysis using SPOT-6 images yielded the best results. This was due to the high spatial resolution of SPOT-6 compared to Landsat and Support Vector Machine classifier was able to classify images with fewer training points compared to Random Forest. The spatio-temporal land cover change at the mine was then determined between the years 2000, 2015 and 2020. Land cover classification is useful for monitoring land degradation and, in this case, was able to show the extent of rehabilitation success. For the year 2020, a 17% area was rehabilitated; however, the algorithm could not distinguish between unmined Calcrete Bontveld matrix and rehabilitation sites that were older than five years. The performed change detection also showed that 29.50% of unmined Calcrete Bontveld matrix had changed to “mature rehabilitation” (rehabilitation sites older than five years). Again, for this percentage in some areas the algorithm could not distinguish between the unmined Calcrete Bontveld matrix and rehabilitation sites that were older than five years due to high similarities between the two land cover types. Area changes of the different land cover classes could also be used to demonstrate how rehabilitation areas have matured over time and lead to the conclusion that most of the Calcrete Bontveld which was mined, has over the years been successfully rehabilitated. Vegetation analysis was conducted to further validate the rehabilitation success of Calcrete Bontveld matrix. Multivariant Detrended Correspondent Analysis showed that rehabilitation sites which were younger than five years (2–year-old rehabilitation plots that were sampled) had great dissimilarity to the natural unmined Calcrete Bontveld matrix and that rehabilitation sites older than five years, in this case 16–years older, had high similarity and resemblance to natural unmined Calcrete Bontveld matrix and therefore could be considered as being mature. This was a more definitive assessment as it considers all aspects of the vegetation. Species cover and species richness also showed that Calcrete Bontveld matrix rehabilitation sites which have been rehabilitated for more than 5 years had greater similarity to natural unmined vegetation compared to areas that have been rehabilitated for less than five years. This study, therefore, demonstrates that due to the high similarity between mature rehabilitation sites and unmined Calcrete Bontveld, rehabilitation has been successful. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
Assessing the effectiveness of smart solutions on potable water in rural areas within Ngqushwa local municipality
- Authors: Fodo, Portia Noluthando
- Date: 2023-12
- Subjects: Potable water -- tap water , water infrastructure
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63298 , vital:73245
- Description: Providing potable water is a critical reposition towards achieving the Sustainable Development Goal (SDG) 6, to ensure the availability and sustainable management of water and sanitation for all. In addition, the use of smart solutions facilitates towards guaranteeing smart cities aligned with SDG11 which is aimed at making cities and human settlements inclusive, secure, resilient and viable. Access to potable water is a challenging issue in most rural areas as these areas were affected by the Apartheid regime where services were distributed according to the colour of the skin. In rural areas where many black people reside, the challenge of service delivery still exists. Ngqushwa Municipality is faced with several trials in the management of water, including water scarcity, lack of water infrastructure as well as illegal water connections. Despite more than 25 years of democracy, rural dwellers in Ngqushwa Municipality still suffer from a deficiency of access to potable water, which results in other health problems, such as Cholera. This study was aimed at assessing the role of smart solutions in the effective management of potable water in rural areas in Ngqushwa Local Municipality. The objectives to be achieved include identification of the policies that support the implementation of smart solutions in the provision of potable water in Ngqushwa Local Municipality, as well as determining the opportunities and challenges associated with smart solutions in the delivery of potable water. Moreover, identifying the existing difficulties affecting the execution of smart solutions in the delivery of potable water, along with developing recommendations on how smart solutions can effectively be put into operation in rural areas to control water. A qualitative research approach was applied as the chosen research methodology most appropriate to achieving the objectives of the research study. Data was collected using secondary sources and was analysed using thematic content analysis. The study revealed that opportunities available include accurate billing, smart metering and information availability. Furthermore, the obstacles included capacity constraints, cost factors and resistance to change. The investigation recommended, inter alia, that the municipality should accurately train its employees on matters relating to change management and establish effective debt controlling mechanisms to enhance water supervision in rural areas. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Fodo, Portia Noluthando
- Date: 2023-12
- Subjects: Potable water -- tap water , water infrastructure
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63298 , vital:73245
- Description: Providing potable water is a critical reposition towards achieving the Sustainable Development Goal (SDG) 6, to ensure the availability and sustainable management of water and sanitation for all. In addition, the use of smart solutions facilitates towards guaranteeing smart cities aligned with SDG11 which is aimed at making cities and human settlements inclusive, secure, resilient and viable. Access to potable water is a challenging issue in most rural areas as these areas were affected by the Apartheid regime where services were distributed according to the colour of the skin. In rural areas where many black people reside, the challenge of service delivery still exists. Ngqushwa Municipality is faced with several trials in the management of water, including water scarcity, lack of water infrastructure as well as illegal water connections. Despite more than 25 years of democracy, rural dwellers in Ngqushwa Municipality still suffer from a deficiency of access to potable water, which results in other health problems, such as Cholera. This study was aimed at assessing the role of smart solutions in the effective management of potable water in rural areas in Ngqushwa Local Municipality. The objectives to be achieved include identification of the policies that support the implementation of smart solutions in the provision of potable water in Ngqushwa Local Municipality, as well as determining the opportunities and challenges associated with smart solutions in the delivery of potable water. Moreover, identifying the existing difficulties affecting the execution of smart solutions in the delivery of potable water, along with developing recommendations on how smart solutions can effectively be put into operation in rural areas to control water. A qualitative research approach was applied as the chosen research methodology most appropriate to achieving the objectives of the research study. Data was collected using secondary sources and was analysed using thematic content analysis. The study revealed that opportunities available include accurate billing, smart metering and information availability. Furthermore, the obstacles included capacity constraints, cost factors and resistance to change. The investigation recommended, inter alia, that the municipality should accurately train its employees on matters relating to change management and establish effective debt controlling mechanisms to enhance water supervision in rural areas. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
Assessing the effectiveness of the planning, utilisation and implementation of the electronic human resource management strategy: a case of the department of home affairs in the Eastern Cape
- Authors: Dyan, Margaret Nombuyiselo
- Date: 2023-12
- Subjects: Personnel management , Home Affairs -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63287 , vital:73244
- Description: This study assessed the effectiveness of planning, implementing and utilising the electronic human resource management (e-HRM) strategy within the King William’s Town (KWT) Department of Home Affairs (DHA) in the Eastern Cape Province (EC) of South Africa (SA). E-HRM emerged because of the advent of technology. Many organisations use information technology to optimise the performance of both the employees and the organisation. Thus, this technology has been incorporated into organisations’ human resource functions, taking advantage of the latest technology to deliver an online real-time human resource management (HRM) solution. Therefore, this study sought to assess the e-HRM strategy's effectiveness within the KWT DHA. The technology acceptance model was adopted as a theoretical framework for the study to explain the adoption of the e-HRM strategy. A qualitative research approach using a case study research design was used for this study. Non-probability purposive sampling and convenience sampling were used to identify participants for the study. These participants provided the information needed to answer the research questions. The data was collected using in-depth interviews with the participants. The data was then analysed using thematic analysis following Braun and Clarke’s guidelines (2006). The study results revealed that implementing the e-HRM strategy within KWT DHA proves to be effective and efficient. The strategy’s implementation has been associated with improved performance of the KWT DHA in the Eastern Cape. It also saves time and ensures that HR information is accessed easily. The study also found barriers to the planning, implementation and utilisation of the e-HRM strategy, including a lack of skills and knowledge and resource constraints. The study also found that the current e-HRM policies are not fully functional. The study recommends continuous personnel training regarding the strategy, maximising the use of the e-HRM strategy and addressing the resource challenges. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Dyan, Margaret Nombuyiselo
- Date: 2023-12
- Subjects: Personnel management , Home Affairs -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63287 , vital:73244
- Description: This study assessed the effectiveness of planning, implementing and utilising the electronic human resource management (e-HRM) strategy within the King William’s Town (KWT) Department of Home Affairs (DHA) in the Eastern Cape Province (EC) of South Africa (SA). E-HRM emerged because of the advent of technology. Many organisations use information technology to optimise the performance of both the employees and the organisation. Thus, this technology has been incorporated into organisations’ human resource functions, taking advantage of the latest technology to deliver an online real-time human resource management (HRM) solution. Therefore, this study sought to assess the e-HRM strategy's effectiveness within the KWT DHA. The technology acceptance model was adopted as a theoretical framework for the study to explain the adoption of the e-HRM strategy. A qualitative research approach using a case study research design was used for this study. Non-probability purposive sampling and convenience sampling were used to identify participants for the study. These participants provided the information needed to answer the research questions. The data was collected using in-depth interviews with the participants. The data was then analysed using thematic analysis following Braun and Clarke’s guidelines (2006). The study results revealed that implementing the e-HRM strategy within KWT DHA proves to be effective and efficient. The strategy’s implementation has been associated with improved performance of the KWT DHA in the Eastern Cape. It also saves time and ensures that HR information is accessed easily. The study also found barriers to the planning, implementation and utilisation of the e-HRM strategy, including a lack of skills and knowledge and resource constraints. The study also found that the current e-HRM policies are not fully functional. The study recommends continuous personnel training regarding the strategy, maximising the use of the e-HRM strategy and addressing the resource challenges. , Thesis (MA) -- Faculty of Humanities, 2023
- Full Text:
- Date Issued: 2023-12
Assessing the effects of materials management practices on performance of locomotive manufacturing firms in Gauteng province
- Authors: Setino, Rebecca
- Date: 2023-12
- Subjects: Materials management , Industrial procurement , Business logistics -- South Africa -- Gauteng
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/65935 , vital:74288
- Description: The study assessed the effects of materials management practices (materials planning, procurement, availability, handling, and delivery) on the performance of rail rolling stock and locomotive manufacturing firms in Gauteng Province. The study also investigated the causes of delays in supply of materials in the rail rolling stock and locomotive manufacturing industry in Gauteng. The research design employed was both descriptive and explanatory, using a quantitative research approach in which online surveys were conducted on a sample of 200 respondents from the rolling stock manufacturing firms in Gauteng. Descriptive statistics performed through SPSS 27 and SEM performed through AMOS 26 were used for data analysis. The findings of the study revealed that materials management practises were implemented in the rail rolling stock and locomotive manufacturing industry. According to the results, materials availability and materials handling have positive and statistically significant effects on the performance of rail manufacturing firms in Gauteng Province. However, materials planning, procurement and delivery practices have no significant effect on the performance of rail manufacturing firms in Gauteng Province. Therefore, the results reveal that firm performance is driven by materials availability and materials handling. In addition, late delivery of materials to the production line was found to be the main cause of project delays. The study thus offered a contrasting perspective to the general findings that show materials management practices to be a significant contributor to manufacturing firm performance. However, the study confirms the importance of materials handling and availability as a predictor of performance in manufacturing firms. , Thesis (MCom) -- Faculty of Business and Economic Sciences, School of Management Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Setino, Rebecca
- Date: 2023-12
- Subjects: Materials management , Industrial procurement , Business logistics -- South Africa -- Gauteng
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/65935 , vital:74288
- Description: The study assessed the effects of materials management practices (materials planning, procurement, availability, handling, and delivery) on the performance of rail rolling stock and locomotive manufacturing firms in Gauteng Province. The study also investigated the causes of delays in supply of materials in the rail rolling stock and locomotive manufacturing industry in Gauteng. The research design employed was both descriptive and explanatory, using a quantitative research approach in which online surveys were conducted on a sample of 200 respondents from the rolling stock manufacturing firms in Gauteng. Descriptive statistics performed through SPSS 27 and SEM performed through AMOS 26 were used for data analysis. The findings of the study revealed that materials management practises were implemented in the rail rolling stock and locomotive manufacturing industry. According to the results, materials availability and materials handling have positive and statistically significant effects on the performance of rail manufacturing firms in Gauteng Province. However, materials planning, procurement and delivery practices have no significant effect on the performance of rail manufacturing firms in Gauteng Province. Therefore, the results reveal that firm performance is driven by materials availability and materials handling. In addition, late delivery of materials to the production line was found to be the main cause of project delays. The study thus offered a contrasting perspective to the general findings that show materials management practices to be a significant contributor to manufacturing firm performance. However, the study confirms the importance of materials handling and availability as a predictor of performance in manufacturing firms. , Thesis (MCom) -- Faculty of Business and Economic Sciences, School of Management Sciences, 2023
- Full Text:
- Date Issued: 2023-12
Assessment of knowledge, attitudes, practices and the prevalence of intestinal parasites in Sarah Baartman District, Eastern Cape
- Authors: Mrwebi, Sisanda
- Date: 2023-12
- Subjects: Intestinal parasites , Knowledge Attitudes and Practices , transmitted helminths
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62924 , vital:73070
- Description: ntestinal parasites also known as parasitic worms are one of the common water, soil and feacal transmitted infections of public importance in the world. This study evaluated the Knowledge, Attitudes and Practices (KAP) of public workers comprising EPWP and CWP general workers on intestinal parasites in a selected municipality in the study area . Additionally, using secondary data, the researcher established the prevalence of intestinal parasites in Sarah Baartman District Municipality (SBDM) from 2012 to 2020. Method: The study made use of a quantitative, cross-sectional research design resembling a study by S.D. Hambury .A structured questionnaire, closed-ended, telephonically-administered questionnaire was used to collect data from 160 Expanded Public Works Programme (EPWP) and Community Work Programme (CWP) general workers. Data on the prevalence was obtained from the SBDM National Health Laboratory Service (NHLS). Data was analysed using both inferential and descriptive statistics using SPSS software version 16.1, inferential statistics tests used were Chi2 square tests and p-value tests. Descriptive statistic tests used included frequency distributions, percentage, cumulative percent, and valid percent. Results: The participants’ overall awareness, attitudes and practices of the study were poor, 64.4% of the study participants had the correct knowledge about intestinal parasite infections. The age of study participants and who the participants thought was at risk of being infected by intestinal parasites had a non significant relationship (p>0.05). Gender and the responses to who is at risk of getting infected with intestinal parasites had a statistically significant (p<0.05) relationship. Intestinal parasite prevalence from 2012 to 2020 in SBDM was 46.37% which means it was close to half of the sampled population. Males had the highest prevalence as compared to females, with a prevalence of 53.1% xvi in males and 46.9% in females. The highest disease infection was found between ages one and six years. Conclusion: The researcher concluded that the overall awareness, attitudes and practices on intestinal parasites in SBDM were poor. The intestinal parasites present in SBDM were Cytoisospora belli (35.4%) and Giardia lamblia (35.4%), Ascaris lumbricoides (17.7%), Trichuris trichiura (3%), and Taenia species (2%). The study also revealed that the prevalence of intestinal parasites was low from 2012 to 2020. Recommendations were made with an emphasis on education to increase disease prevention and control awareness. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Mrwebi, Sisanda
- Date: 2023-12
- Subjects: Intestinal parasites , Knowledge Attitudes and Practices , transmitted helminths
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62924 , vital:73070
- Description: ntestinal parasites also known as parasitic worms are one of the common water, soil and feacal transmitted infections of public importance in the world. This study evaluated the Knowledge, Attitudes and Practices (KAP) of public workers comprising EPWP and CWP general workers on intestinal parasites in a selected municipality in the study area . Additionally, using secondary data, the researcher established the prevalence of intestinal parasites in Sarah Baartman District Municipality (SBDM) from 2012 to 2020. Method: The study made use of a quantitative, cross-sectional research design resembling a study by S.D. Hambury .A structured questionnaire, closed-ended, telephonically-administered questionnaire was used to collect data from 160 Expanded Public Works Programme (EPWP) and Community Work Programme (CWP) general workers. Data on the prevalence was obtained from the SBDM National Health Laboratory Service (NHLS). Data was analysed using both inferential and descriptive statistics using SPSS software version 16.1, inferential statistics tests used were Chi2 square tests and p-value tests. Descriptive statistic tests used included frequency distributions, percentage, cumulative percent, and valid percent. Results: The participants’ overall awareness, attitudes and practices of the study were poor, 64.4% of the study participants had the correct knowledge about intestinal parasite infections. The age of study participants and who the participants thought was at risk of being infected by intestinal parasites had a non significant relationship (p>0.05). Gender and the responses to who is at risk of getting infected with intestinal parasites had a statistically significant (p<0.05) relationship. Intestinal parasite prevalence from 2012 to 2020 in SBDM was 46.37% which means it was close to half of the sampled population. Males had the highest prevalence as compared to females, with a prevalence of 53.1% xvi in males and 46.9% in females. The highest disease infection was found between ages one and six years. Conclusion: The researcher concluded that the overall awareness, attitudes and practices on intestinal parasites in SBDM were poor. The intestinal parasites present in SBDM were Cytoisospora belli (35.4%) and Giardia lamblia (35.4%), Ascaris lumbricoides (17.7%), Trichuris trichiura (3%), and Taenia species (2%). The study also revealed that the prevalence of intestinal parasites was low from 2012 to 2020. Recommendations were made with an emphasis on education to increase disease prevention and control awareness. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
Characterization of the coastal Aquifer of Gqeberha, South Africa : Developing a conceptual understanding
- Authors: Vandala, Bamanye
- Date: 2023-12
- Subjects: Aquifers -- South Africa -- Port Elizabeth , Groundwater ecology , Hydrogeology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62710 , vital:72932
- Description: The aquifer systems in Gqeberha are poorly understood due to a lack of groundwater studies within the area, and this is particularly the case for the coastal aquifer of Gqeberha. Amidst the water crisis the city is currently facing, groundwater has become crucial, with over 1000 boreholes reportedly drilled in the coastal area, most of which are residential. This has resulted in the need to conduct more groundwater studies in the area and in Nelson Mandela Bay. This study presents a detailed hydrogeological characterization of the coastal aquifer of Gqeberha. This was achieved through the use of different techniques, which include field mapping, geophysics, hydrochemistry, and pumping test analysis. The work was done on the coastal seeps of the Algoa Group Formation and the fractured aquifer of the Peninsula Formation. The results through mapping indicated an abundance of folds, faults, and fractures which all have implications for groundwater flow. The majority of fractures are striking in the NE-SW and NW-SE directions. The pumping test analysis also indicated fractal flow with significant matrix flow resulting in a dual porosity system up to at least 200m in depth. The transmissivity and storativity values range from 6.78 – 44.5 m2/day and 0.0172 – 0.0325, respectively. This is highly dependent on the fracture interconnectivity, which varies across the area and is higher at shallow depths, as suggested by the geophysical profiles. Geophysical profiles indicate that the contact between the Algoa Group Formations and the Peninsula Formation results in coastal seeps due to the difference in hydraulic conductivities. The hydrochemical analysis suggests that the seeps generally have Na-Cl and Ca-Mg-Cl water types. The groundwater in the eastern area has a Ca-HCO3 water type, and the groundwater in the western region has a Ca-Mg-Cl water type, with the Chelsea-Noordhoek Fault system separating the two groundwater signatures. Groundwater at greater depths has a Na-Cl water type with higher TDS values. The chemical signatures seen are due to external factors, which include sea sprays, anthropogenic activities, rock-water processes, and possibly connate water in the Peninsula Formation aquifer. The findings of this study offer valuable insights into the present conditions of the aquifer in Gqeberha. This research serves as a foundational study that can provide a baseline for future hydrogeological investigations in the area. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Vandala, Bamanye
- Date: 2023-12
- Subjects: Aquifers -- South Africa -- Port Elizabeth , Groundwater ecology , Hydrogeology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62710 , vital:72932
- Description: The aquifer systems in Gqeberha are poorly understood due to a lack of groundwater studies within the area, and this is particularly the case for the coastal aquifer of Gqeberha. Amidst the water crisis the city is currently facing, groundwater has become crucial, with over 1000 boreholes reportedly drilled in the coastal area, most of which are residential. This has resulted in the need to conduct more groundwater studies in the area and in Nelson Mandela Bay. This study presents a detailed hydrogeological characterization of the coastal aquifer of Gqeberha. This was achieved through the use of different techniques, which include field mapping, geophysics, hydrochemistry, and pumping test analysis. The work was done on the coastal seeps of the Algoa Group Formation and the fractured aquifer of the Peninsula Formation. The results through mapping indicated an abundance of folds, faults, and fractures which all have implications for groundwater flow. The majority of fractures are striking in the NE-SW and NW-SE directions. The pumping test analysis also indicated fractal flow with significant matrix flow resulting in a dual porosity system up to at least 200m in depth. The transmissivity and storativity values range from 6.78 – 44.5 m2/day and 0.0172 – 0.0325, respectively. This is highly dependent on the fracture interconnectivity, which varies across the area and is higher at shallow depths, as suggested by the geophysical profiles. Geophysical profiles indicate that the contact between the Algoa Group Formations and the Peninsula Formation results in coastal seeps due to the difference in hydraulic conductivities. The hydrochemical analysis suggests that the seeps generally have Na-Cl and Ca-Mg-Cl water types. The groundwater in the eastern area has a Ca-HCO3 water type, and the groundwater in the western region has a Ca-Mg-Cl water type, with the Chelsea-Noordhoek Fault system separating the two groundwater signatures. Groundwater at greater depths has a Na-Cl water type with higher TDS values. The chemical signatures seen are due to external factors, which include sea sprays, anthropogenic activities, rock-water processes, and possibly connate water in the Peninsula Formation aquifer. The findings of this study offer valuable insights into the present conditions of the aquifer in Gqeberha. This research serves as a foundational study that can provide a baseline for future hydrogeological investigations in the area. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
Chemical analysis and biological activities of crude extracts and essential oil of selected medicinal plants from the Eastern Cape, South Africa, and Volta Region of Ghana
- Authors: Agbo, Irene Adzo
- Date: 2023-12
- Subjects: Medicinal plants , Lantana camara , Peptic ulcer -- Treatment , Traditional medicine - South Africa -- Eastern Cape , Traditional medicine -- Ghana
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62431 , vital:72728
- Description: Lantana camara and Khaya grandifoliola extracts are among many plants found traditionally effective for the treatment of wounds and ulcers. This study assessed the phytochemical content, isolation and identification of single compounds from methanol and ethyl acetate extracts of Lantana camara and Khaya grandifoliola. Further, the bioactivity testing including antioxidant, antimicrobial and cytoxicity of the extracts was done to confirm the wound healing potential discovered by the traditional healers. Materials and methods: Extraction was done successively using maceration method with 100 % ethyl acetate and 100 % methanol with a biomass-to-solvent ratio of 1:3 (w/v) to obtain L. camara ethyl acetate extracts of berry (ELB), flower (ELF) and leaf (ELL) and methanol extracts of MLB, MLF, MLL and K. grandifoliola ethyl acetate extracts of leaf (EKL), root (EKR) and stem bark (EKSB) and methanol extracts of MKL, MKR, MKSB respectively. L. camara leaf essential oil (EO) was extracted using the hydro-distillation method with a Clevenger apparatus. Total phytochemical content was assessed for each extract using spectrophotometric methods and a calibration curve of standards: bromocresol green method with atropine; Folin–Ciocalteu colorimetric method with gallic acid, aluminium chloride colorimetric method with quercetin and concentrated sulphuric acid chloroform with linalool for total alkaloid, phenolic, flavonoid and terpenoid contents respectively. Single compound isolation and purification was conducted using chromatographic techniques. Elucidation of single compounds was done using spectrometric method, high resolution- mass spectrometry, and one and two-dimensional (1D and 2D)-NMR. Stereochemistry of each compound was confirmed using electronic circular dichroism spectra. A Crystalline compound was identified by single crystal X-ray diffraction using CuKα-radiation. In vitro bioactivities were assessed with methods such as 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide, free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl, inhibitory effect on nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages, and 96-well plate micro dilution for cytotoxicity, ant-inflammatory and antimicrobial activity testing. Results: Methanol extracts of both plants retained high phytochemical concentrations of all the phytoconstituents investigated compared with the ethyl acetate extracts which retained lower concentrations. The results of the L. camara methanol extracts include; total alkaloid content (TAC) (2.05±0.18, 1.87±1.54 and 2.60±1.10 mg AEQ/100 mg); total phenolic content (TPC) (14.05±4.04, 34.59±3.01 and 18.58±1.87 mg GAEQ/100 mg); the total flavonoid content(TFC) of flower (12.45±1.87, 20.41±2.69 and mg QEQ/100 mg); total terpenoids (TTC) (20.74±2.34, 20.74±2.34 and 15.97±1.19 mg LIN EQ/100mg) of MLB, MLF and MLL respectively. Whereas that of the K. grandifoliola methanol extracts include; TAC (7.32±0.14,8.49±0.34, 10.67±0.22 mg AEQ/100 mg); TPC (37.49±1.40, 44.41±0.69, 53.57±1.50 mgGAEQ/100 mg); TFC (6.54±0.55, 9.58±0.89 and 10.26±0.92 mg QEQ/100 mg); TTC(10.16±1.41, 35.78±2.14 and 23.45±1.76 mg LIN EQ/100mg) of MKL, MKR and MKSB respectively. The major components of essential oil, out of the 71 constituents identified include Davanone D (32.91 %), Caryophyllene (5.07 %), Nerolidol 2 (3.56 %) and GermacreneD (3.13 %). Compounds 3.47 was isolated from the methanol extract of L. camara flowers. This compound is reported for the first time from the L. camara flower extract. Two compounds, compounds 4.23, and 4.26, were isolated from the methanol extract of K. grandifoliola roots, compound 4.22 was isolated from the ethyl acetate root extract while compounds 4.24 and 4.25 were isolated from the ethyl acetate stem bark extract as isomers in a mixture. Compounds 4.22 and 4.23 are reported from K. grandifoliola root for the first time. The isolated compounds (compounds 3.47 and 4.23) were nontoxic to the Vero cell line and this may contribute to possible stimulation of cell proliferation, promoting wound healing. Cytotoxicity describes extract virulence to Vero cell line. MLF and ELB were found nontoxic even at the highest concentration of 200 μg/mL. The MKSB and MKR, as well as the EKSB were nontoxic. Antioxidant activity results, described by the percentage inhibition in the DPPH assay, showed that MLF and MKSB had the highest antioxidant activities compared with the ascorbic acid standard, with IC50 of 38.68±5.09 and 37.03±11.95 μg/mL for L. camara and K. grandifoliola respectively. ELB exhibited a significant anti-inflammatory activity inhibiting NO• radical generation in the LPS-stimulated RAW 264.7 macrophages at concentration ranging from 50 and 100 μg/mL. EKSB and MKR showed significant anti-inflammatory activity at 100 and 200 μg/ml respectively. ELL and ELF demonstrated potent growth inhibition against S. pyogenes with an MIC value ≤ 0.125 mg/mL, while the MICs of the ELB and MLL were 0.5 mg/mL and 2 mg/mL respectively. MKSB and MKR and EKSB extract exhibited an effective growth inhibition against S. aureus with MIC of 1 mg/mL. The growth of S. pyogenes was supressed by both ethyl acetate and methanol extracts of all plant parts tested with MIC ranging from 0.25–2 mg/mL. Conclusion: The potent bioactivity shown in the results of the cytotoxicity, antioxidant activity, anti-inflammatory and antimicrobial activity testing, and the nontoxic singlecompounds of L. camara and K. grandifoliola extracts led to the conclusion that the two plants had wound healing potential. The study therefore confirmed their traditional uses for treatment of wounds. , Thesis (PhD) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Agbo, Irene Adzo
- Date: 2023-12
- Subjects: Medicinal plants , Lantana camara , Peptic ulcer -- Treatment , Traditional medicine - South Africa -- Eastern Cape , Traditional medicine -- Ghana
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/62431 , vital:72728
- Description: Lantana camara and Khaya grandifoliola extracts are among many plants found traditionally effective for the treatment of wounds and ulcers. This study assessed the phytochemical content, isolation and identification of single compounds from methanol and ethyl acetate extracts of Lantana camara and Khaya grandifoliola. Further, the bioactivity testing including antioxidant, antimicrobial and cytoxicity of the extracts was done to confirm the wound healing potential discovered by the traditional healers. Materials and methods: Extraction was done successively using maceration method with 100 % ethyl acetate and 100 % methanol with a biomass-to-solvent ratio of 1:3 (w/v) to obtain L. camara ethyl acetate extracts of berry (ELB), flower (ELF) and leaf (ELL) and methanol extracts of MLB, MLF, MLL and K. grandifoliola ethyl acetate extracts of leaf (EKL), root (EKR) and stem bark (EKSB) and methanol extracts of MKL, MKR, MKSB respectively. L. camara leaf essential oil (EO) was extracted using the hydro-distillation method with a Clevenger apparatus. Total phytochemical content was assessed for each extract using spectrophotometric methods and a calibration curve of standards: bromocresol green method with atropine; Folin–Ciocalteu colorimetric method with gallic acid, aluminium chloride colorimetric method with quercetin and concentrated sulphuric acid chloroform with linalool for total alkaloid, phenolic, flavonoid and terpenoid contents respectively. Single compound isolation and purification was conducted using chromatographic techniques. Elucidation of single compounds was done using spectrometric method, high resolution- mass spectrometry, and one and two-dimensional (1D and 2D)-NMR. Stereochemistry of each compound was confirmed using electronic circular dichroism spectra. A Crystalline compound was identified by single crystal X-ray diffraction using CuKα-radiation. In vitro bioactivities were assessed with methods such as 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide, free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl, inhibitory effect on nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages, and 96-well plate micro dilution for cytotoxicity, ant-inflammatory and antimicrobial activity testing. Results: Methanol extracts of both plants retained high phytochemical concentrations of all the phytoconstituents investigated compared with the ethyl acetate extracts which retained lower concentrations. The results of the L. camara methanol extracts include; total alkaloid content (TAC) (2.05±0.18, 1.87±1.54 and 2.60±1.10 mg AEQ/100 mg); total phenolic content (TPC) (14.05±4.04, 34.59±3.01 and 18.58±1.87 mg GAEQ/100 mg); the total flavonoid content(TFC) of flower (12.45±1.87, 20.41±2.69 and mg QEQ/100 mg); total terpenoids (TTC) (20.74±2.34, 20.74±2.34 and 15.97±1.19 mg LIN EQ/100mg) of MLB, MLF and MLL respectively. Whereas that of the K. grandifoliola methanol extracts include; TAC (7.32±0.14,8.49±0.34, 10.67±0.22 mg AEQ/100 mg); TPC (37.49±1.40, 44.41±0.69, 53.57±1.50 mgGAEQ/100 mg); TFC (6.54±0.55, 9.58±0.89 and 10.26±0.92 mg QEQ/100 mg); TTC(10.16±1.41, 35.78±2.14 and 23.45±1.76 mg LIN EQ/100mg) of MKL, MKR and MKSB respectively. The major components of essential oil, out of the 71 constituents identified include Davanone D (32.91 %), Caryophyllene (5.07 %), Nerolidol 2 (3.56 %) and GermacreneD (3.13 %). Compounds 3.47 was isolated from the methanol extract of L. camara flowers. This compound is reported for the first time from the L. camara flower extract. Two compounds, compounds 4.23, and 4.26, were isolated from the methanol extract of K. grandifoliola roots, compound 4.22 was isolated from the ethyl acetate root extract while compounds 4.24 and 4.25 were isolated from the ethyl acetate stem bark extract as isomers in a mixture. Compounds 4.22 and 4.23 are reported from K. grandifoliola root for the first time. The isolated compounds (compounds 3.47 and 4.23) were nontoxic to the Vero cell line and this may contribute to possible stimulation of cell proliferation, promoting wound healing. Cytotoxicity describes extract virulence to Vero cell line. MLF and ELB were found nontoxic even at the highest concentration of 200 μg/mL. The MKSB and MKR, as well as the EKSB were nontoxic. Antioxidant activity results, described by the percentage inhibition in the DPPH assay, showed that MLF and MKSB had the highest antioxidant activities compared with the ascorbic acid standard, with IC50 of 38.68±5.09 and 37.03±11.95 μg/mL for L. camara and K. grandifoliola respectively. ELB exhibited a significant anti-inflammatory activity inhibiting NO• radical generation in the LPS-stimulated RAW 264.7 macrophages at concentration ranging from 50 and 100 μg/mL. EKSB and MKR showed significant anti-inflammatory activity at 100 and 200 μg/ml respectively. ELL and ELF demonstrated potent growth inhibition against S. pyogenes with an MIC value ≤ 0.125 mg/mL, while the MICs of the ELB and MLL were 0.5 mg/mL and 2 mg/mL respectively. MKSB and MKR and EKSB extract exhibited an effective growth inhibition against S. aureus with MIC of 1 mg/mL. The growth of S. pyogenes was supressed by both ethyl acetate and methanol extracts of all plant parts tested with MIC ranging from 0.25–2 mg/mL. Conclusion: The potent bioactivity shown in the results of the cytotoxicity, antioxidant activity, anti-inflammatory and antimicrobial activity testing, and the nontoxic singlecompounds of L. camara and K. grandifoliola extracts led to the conclusion that the two plants had wound healing potential. The study therefore confirmed their traditional uses for treatment of wounds. , Thesis (PhD) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
- Full Text:
- Date Issued: 2023-12
Dietary assessment of Cape Gannets (Morus capensis) and African Penguins (Spheniscus demersus) in Algoa Bay using molecular scatology
- Bowmaker - Falconer, Kezia, Von Der Heyden, Sophie
- Authors: Bowmaker - Falconer, Kezia , Von Der Heyden, Sophie
- Date: 2023-12
- Subjects: Gannets , Gannets -- Food , Sea birds -- South Africa -- Bird Island, Algoa Bay.
- Language: English
- Type: Master's theses
- Identifier: http://hdl.handle.net/10948/62420 , vital:72675
- Description: Seabirds are important marine sentinels, providing valuable information about ecosystem health and condition. Dietary studies of seabirds can inform on ecosystem functioning through identifying the condition and changes occurring in their surrounding environment. Dietary studies are also crucial as they can identify threats that seabirds face. Until recently, the diets of southern African endemic and Endangered Cape Gannets Morus capensis and African Penguins Spheniscus demersus were solely investigated through stomach content analyses, which allows for the identification and quantification of prey remains. To overcome some of the limitations associated with stomach content analysis, stable isotope and fatty acid analyses were proposed. However, these methods still require bird handling and thus impose some stress on the birds. DNA metabarcoding of faecal matter shows promise as a completely noninvasive method for seabird dietary studies. This thesis first examined whether DNA metabarcoding could be used to determine the diet of Cape Gannet and African Penguin breeding in Algoa Bay, South Africa. Then, this method was used to investigate the influence of intrinsic (both seabird species) and extrinsic (African Penguins) factors on the diet of the two seabird species. For the first data chapter, stomach content samples and faecal matter were collected concurrently from breeding Cape Gannets and African Penguins on Bird Island. DNA was successfully extracted from most faecal matter samples and the primers (18S_SSU, 16S_Fish) used for group, taxa and fish prey identification allowed amplification of prey DNA for both study species. A similar piscivorous diet was determined by both dietary methods for Cape Gannets with Sardine Sardinops sagax and Atlantic Saury Scomberesox saurus dominating the diet. Sardine also dominated the African Penguin diet in both dietary methods. In addition, DNA metabarcoding in African Penguins revealed a more diverse diet compared to the one determined by stomach content analysis. This was likely due to the limitations on the allowed number of stomach content samples to be collected for that Endangered species. Non-invasive DNA metabarcoding of faecal matter was then used to investigate whether the diet varied with breeding stages (incubation, chick-rearing) and age classes (chick-rearing adults, chicks) in both seabird species, but also breeding colonies (Algoa Bay: Bird Island, St Croix Island) in African Penguins. DNA metabarcoding revealed that breeding stages influenced the diet of Cape Gannets and African Penguins on Bird Island where prey species diversity was greater for incubating adults. On the contrary, the diet of incubating and chick-rearing penguins on St Croix Island was similar. When comparing the diet of African Penguins between Bird Island and St Croix Island, there was an increased overlap in diet composition between incubating and chick-rearing adults on St Croix Island when compared to Bird Island. Importantly, DNA metabarcoding indicated an increase in fishery interactions in Algoa Bay, particularly for Cape Gannets and the hake Merluccius spp. fishery, where the contribution of hake to chick-rearing adults and chick diet was considerably greater than incubating adult diet. In this study, I showed that DNA metabarcoding of faecal matter provides an opportunity for non-invasive dietary studies in two southern African endemic and Endangered seabird species. Incorporating non-invasive methods as a complement to other dietary methods for monitoring seabird trophic ecology is crucial when considering the worsening status of several seabird species. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Bowmaker - Falconer, Kezia , Von Der Heyden, Sophie
- Date: 2023-12
- Subjects: Gannets , Gannets -- Food , Sea birds -- South Africa -- Bird Island, Algoa Bay.
- Language: English
- Type: Master's theses
- Identifier: http://hdl.handle.net/10948/62420 , vital:72675
- Description: Seabirds are important marine sentinels, providing valuable information about ecosystem health and condition. Dietary studies of seabirds can inform on ecosystem functioning through identifying the condition and changes occurring in their surrounding environment. Dietary studies are also crucial as they can identify threats that seabirds face. Until recently, the diets of southern African endemic and Endangered Cape Gannets Morus capensis and African Penguins Spheniscus demersus were solely investigated through stomach content analyses, which allows for the identification and quantification of prey remains. To overcome some of the limitations associated with stomach content analysis, stable isotope and fatty acid analyses were proposed. However, these methods still require bird handling and thus impose some stress on the birds. DNA metabarcoding of faecal matter shows promise as a completely noninvasive method for seabird dietary studies. This thesis first examined whether DNA metabarcoding could be used to determine the diet of Cape Gannet and African Penguin breeding in Algoa Bay, South Africa. Then, this method was used to investigate the influence of intrinsic (both seabird species) and extrinsic (African Penguins) factors on the diet of the two seabird species. For the first data chapter, stomach content samples and faecal matter were collected concurrently from breeding Cape Gannets and African Penguins on Bird Island. DNA was successfully extracted from most faecal matter samples and the primers (18S_SSU, 16S_Fish) used for group, taxa and fish prey identification allowed amplification of prey DNA for both study species. A similar piscivorous diet was determined by both dietary methods for Cape Gannets with Sardine Sardinops sagax and Atlantic Saury Scomberesox saurus dominating the diet. Sardine also dominated the African Penguin diet in both dietary methods. In addition, DNA metabarcoding in African Penguins revealed a more diverse diet compared to the one determined by stomach content analysis. This was likely due to the limitations on the allowed number of stomach content samples to be collected for that Endangered species. Non-invasive DNA metabarcoding of faecal matter was then used to investigate whether the diet varied with breeding stages (incubation, chick-rearing) and age classes (chick-rearing adults, chicks) in both seabird species, but also breeding colonies (Algoa Bay: Bird Island, St Croix Island) in African Penguins. DNA metabarcoding revealed that breeding stages influenced the diet of Cape Gannets and African Penguins on Bird Island where prey species diversity was greater for incubating adults. On the contrary, the diet of incubating and chick-rearing penguins on St Croix Island was similar. When comparing the diet of African Penguins between Bird Island and St Croix Island, there was an increased overlap in diet composition between incubating and chick-rearing adults on St Croix Island when compared to Bird Island. Importantly, DNA metabarcoding indicated an increase in fishery interactions in Algoa Bay, particularly for Cape Gannets and the hake Merluccius spp. fishery, where the contribution of hake to chick-rearing adults and chick diet was considerably greater than incubating adult diet. In this study, I showed that DNA metabarcoding of faecal matter provides an opportunity for non-invasive dietary studies in two southern African endemic and Endangered seabird species. Incorporating non-invasive methods as a complement to other dietary methods for monitoring seabird trophic ecology is crucial when considering the worsening status of several seabird species. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
- Full Text:
- Date Issued: 2023-12
Effects of selected natural flavonoids on an optimized insulin resistance induction model on myotubes and hepatocytes in vitro
- Authors: Van de Venter, Ruben
- Date: 2023-12
- Subjects: Flavonoid , Insulin resistance , Myotubes and hepatocytes
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63049 , vital:73085
- Description: Introduction: As the third largest life-threatening non-communicable disease, further research to improve T2DM treatment remains pivotal. The approved insulin sensitizing agents, metformin and thiazolidinediones, although effective, have an array of adverse effects. This leads to the growing need for safer insulin sensitizing agents. Aims: To induce insulin resistance, an insulin resistance induction (IRI) model consisting of palmitic acid (PA), fructose, and dexamethasone (DEX), was optimized. The model was utilized to compare the insulin sensitizing efficacy of chrysin, apigenin, luteolin, and quercetin. The most effective flavonoid was extrapolated based on the hydroxylation hypothesis and relevant SAR. The flavonoid was combined with metformin to evaluate potential insulin sensitizing synergism. Methods: C3A hepatocytes and L6 myotubes were exposed to the IRI models: IR1, IR2, and OIR, for 24 and 48h. Morphological features indicative of insulin resistance were monitored through high-content analysis (HCA). Thereafter, cells were exposed to OIR and concurrently treated with the selected flavonoids. Cytotoxicity, oxidative stress (OS), mitochondrial content, mitochondrial membrane potential (MMP), and lipid accumulation, were once again evaluated through HCA. Subsequently, quercetin was combined with metformin (QM); insulin resistant conditions established through OIR exposure, and QM administered as prophylaxis. The efficacy of QM was determined through changes in phosphorylated-Akt, glucose uptake, and gluconeogenesis. Results: IR1 and IR2 demonstrated excessive potency, whereas OIR consistently generated insulin resistant C3A/L6 cells by increasing OS (↑14%/20%), lipid accumulation (0.7-fold/1.5-fold), and decreasing MMP (↓4%/6%). Of the selected flavonoids, quercetin was most effective at ameliorating each of these parameters. Furthermore, QM demonstrated potential insulin sensitizing synergism, as it improved Akt phosphorylation (Thr308: ↑31%/↑17%; Ser473: ↑29%), glucose uptake (↑10%/ 5%), and suppressed hepatic gluconeogenesis (15↓%) more effectively compared to monotherapy. Conclusions: OIR is an effective model for the induction of complete early-stage insulin resistance in myotubes and hepatocytes. Quercetin demonstrates exceptional antidiabetic activity due its unique expression of hydroxyl groups. Lastly, QM, comparative to monotherapy, improves insulin sensitivity with enhanced efficacy. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12
- Authors: Van de Venter, Ruben
- Date: 2023-12
- Subjects: Flavonoid , Insulin resistance , Myotubes and hepatocytes
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/63049 , vital:73085
- Description: Introduction: As the third largest life-threatening non-communicable disease, further research to improve T2DM treatment remains pivotal. The approved insulin sensitizing agents, metformin and thiazolidinediones, although effective, have an array of adverse effects. This leads to the growing need for safer insulin sensitizing agents. Aims: To induce insulin resistance, an insulin resistance induction (IRI) model consisting of palmitic acid (PA), fructose, and dexamethasone (DEX), was optimized. The model was utilized to compare the insulin sensitizing efficacy of chrysin, apigenin, luteolin, and quercetin. The most effective flavonoid was extrapolated based on the hydroxylation hypothesis and relevant SAR. The flavonoid was combined with metformin to evaluate potential insulin sensitizing synergism. Methods: C3A hepatocytes and L6 myotubes were exposed to the IRI models: IR1, IR2, and OIR, for 24 and 48h. Morphological features indicative of insulin resistance were monitored through high-content analysis (HCA). Thereafter, cells were exposed to OIR and concurrently treated with the selected flavonoids. Cytotoxicity, oxidative stress (OS), mitochondrial content, mitochondrial membrane potential (MMP), and lipid accumulation, were once again evaluated through HCA. Subsequently, quercetin was combined with metformin (QM); insulin resistant conditions established through OIR exposure, and QM administered as prophylaxis. The efficacy of QM was determined through changes in phosphorylated-Akt, glucose uptake, and gluconeogenesis. Results: IR1 and IR2 demonstrated excessive potency, whereas OIR consistently generated insulin resistant C3A/L6 cells by increasing OS (↑14%/20%), lipid accumulation (0.7-fold/1.5-fold), and decreasing MMP (↓4%/6%). Of the selected flavonoids, quercetin was most effective at ameliorating each of these parameters. Furthermore, QM demonstrated potential insulin sensitizing synergism, as it improved Akt phosphorylation (Thr308: ↑31%/↑17%; Ser473: ↑29%), glucose uptake (↑10%/ 5%), and suppressed hepatic gluconeogenesis (15↓%) more effectively compared to monotherapy. Conclusions: OIR is an effective model for the induction of complete early-stage insulin resistance in myotubes and hepatocytes. Quercetin demonstrates exceptional antidiabetic activity due its unique expression of hydroxyl groups. Lastly, QM, comparative to monotherapy, improves insulin sensitivity with enhanced efficacy. , Thesis (Ma) -- Faculty of Health Science, 2023
- Full Text:
- Date Issued: 2023-12