Baba wethu
- St Joseph's Choir, Composer Not Specified, Dargie, Dave
- Authors: St Joseph's Choir , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Zulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/309743 , vital:59073 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD119-08
- Description: Music Festival Performance.
- Full Text: false
- Date Issued: 1988
- Authors: St Joseph's Choir , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Zulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/309743 , vital:59073 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD119-08
- Description: Music Festival Performance.
- Full Text: false
- Date Issued: 1988
Baba wethu
- St Joseph's Choir, Composer not specified, Dargie, Dave
- Authors: St Joseph's Choir , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Sacred music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa, Pretoria sa
- Language: isiZulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/440851 , vital:73822 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DDC182b-08
- Description: Unaccompanied church music festival, liturgical dance and composition of new song
- Full Text: false
- Date Issued: 1988
- Authors: St Joseph's Choir , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Sacred music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa, Pretoria sa
- Language: isiZulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/440851 , vital:73822 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DDC182b-08
- Description: Unaccompanied church music festival, liturgical dance and composition of new song
- Full Text: false
- Date Issued: 1988
Bakreste a re rataneny ka lorato
- Church Music Workshop Participants, Composer Not Specified, Dargie, Dave
- Authors: Church Music Workshop Participants , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Mahalapye f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/312747 , vital:59413 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD129-09
- Description: Practise and performance of church hymn, accompanied by the Marimba.
- Full Text: false
- Date Issued: 1988
- Authors: Church Music Workshop Participants , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Mahalapye f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/312747 , vital:59413 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD129-09
- Description: Practise and performance of church hymn, accompanied by the Marimba.
- Full Text: false
- Date Issued: 1988
Balumeli nu sikuluhe
- Our Lady of Africa Congregation, Composer not specified, Simasiku, John Nyambe, Dargie, Dave
- Authors: Our Lady of Africa Congregation , Composer not specified , Simasiku, John Nyambe , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307232 , vital:58779 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD114-34
- Description: Sung Mass at Our Lady of Africa.
- Full Text: false
- Date Issued: 1988
- Authors: Our Lady of Africa Congregation , Composer not specified , Simasiku, John Nyambe , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307232 , vital:58779 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD114-34
- Description: Sung Mass at Our Lady of Africa.
- Full Text: false
- Date Issued: 1988
Banana, a mu tame ku to utwa
- Ngwezi Catholic Church Congregation, Composer not specified, Dargie, Dave
- Authors: Ngwezi Catholic Church Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307525 , vital:58814 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-04
- Description: Church hymn, accompanied by drums and rattles.
- Full Text: false
- Date Issued: 1988
- Authors: Ngwezi Catholic Church Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307525 , vital:58814 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-04
- Description: Church hymn, accompanied by drums and rattles.
- Full Text: false
- Date Issued: 1988
Banhloile Bakgalabje
- Mafogo, Lukas, Shaai, Phineas, Composer Not Specified, Dargie, Dave
- Authors: Mafogo, Lukas , Shaai, Phineas , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Northern Sotho, Pedi, Sepedi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/311743 , vital:59300 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD125-11
- Description: Traditional Pedi performance played with the Mbira.
- Full Text: false
- Date Issued: 1988
- Authors: Mafogo, Lukas , Shaai, Phineas , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Northern Sotho, Pedi, Sepedi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/311743 , vital:59300 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD125-11
- Description: Traditional Pedi performance played with the Mbira.
- Full Text: false
- Date Issued: 1988
Basilika
- Music Workshop Group, Composer Not Specified, Dargie, Dave
- Authors: Music Workshop Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Sesotho sa Leboa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/311514 , vital:59272 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD125-01
- Description: Practise and performance of church hymn.
- Full Text: false
- Date Issued: 1988
- Authors: Music Workshop Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Sesotho sa Leboa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/311514 , vital:59272 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD125-01
- Description: Practise and performance of church hymn.
- Full Text: false
- Date Issued: 1988
Bawo Thixo Somandla
- Marimba Group, Composer Not Specified, Dargie, Dave
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Munich gw
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316821 , vital:59869 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD140-07
- Description: Church hymn, accompanied by the Marimba.
- Full Text: false
- Date Issued: 1988
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Munich gw
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316821 , vital:59869 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD140-07
- Description: Church hymn, accompanied by the Marimba.
- Full Text: false
- Date Issued: 1988
Bawo usikhulule
- St Mary's Congregation, Marimba Group, Composer Not Specified, Dargie, Dave
- Authors: St Mary's Congregation , Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304439 , vital:58449 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD109-30
- Description: Church hymn, accompanied by the Marimba and clapping.
- Full Text: false
- Date Issued: 1988
- Authors: St Mary's Congregation , Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304439 , vital:58449 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD109-30
- Description: Church hymn, accompanied by the Marimba and clapping.
- Full Text: false
- Date Issued: 1988
Bawo usikhulule
- St Mary's Congregation, Marimba Group, Composer Not Specified, Dargie, Dave
- Authors: St Mary's Congregation , Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304444 , vital:58450 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD109-31
- Description: Church hymn, accompanied by the Marimba and clapping.
- Full Text: false
- Date Issued: 1988
- Authors: St Mary's Congregation , Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304444 , vital:58450 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD109-31
- Description: Church hymn, accompanied by the Marimba and clapping.
- Full Text: false
- Date Issued: 1988
Bawo usikhulule ebunzimeni
- St Mary's Workshop Participants, Mmango, Mary, Matsepu, Daniel, Tsoeu, Elizabeth, Dargie, Dave
- Authors: St Mary's Workshop Participants , Mmango, Mary , Matsepu, Daniel , Tsoeu, Elizabeth , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304074 , vital:58404 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD108-02
- Description: Church hymn, accompanied by the marimba.
- Full Text: false
- Date Issued: 1988
- Authors: St Mary's Workshop Participants , Mmango, Mary , Matsepu, Daniel , Tsoeu, Elizabeth , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Cape Town f-sa
- Language: isiXhosa
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/304074 , vital:58404 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD108-02
- Description: Church hymn, accompanied by the marimba.
- Full Text: false
- Date Issued: 1988
Bless me O Lord
- Youth Group, Composer Not Specified, Dargie, Dave
- Authors: Youth Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Makguang Outstation f-sa
- Language: Sesotho sa Leboa , English
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/312030 , vital:59332 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD127-02
- Description: Religious song, accompanied by drums.
- Full Text: false
- Date Issued: 1988
- Authors: Youth Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Makguang Outstation f-sa
- Language: Sesotho sa Leboa , English
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/312030 , vital:59332 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD127-02
- Description: Religious song, accompanied by drums.
- Full Text: false
- Date Issued: 1988
Bondate baka ba ni lumile
- Ngwezi Catholic Church Congregation, Composer not specified, Dargie, Dave
- Authors: Ngwezi Catholic Church Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307648 , vital:58827 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-08
- Description: Church hymn, accompanied by drums and rattles.
- Full Text: false
- Date Issued: 1988
- Authors: Ngwezi Catholic Church Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307648 , vital:58827 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-08
- Description: Church hymn, accompanied by drums and rattles.
- Full Text: false
- Date Issued: 1988
Cardinal Prayer
- Marimba Group, Composer Not Specified, Dargie, Dave
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Ursberg gw
- Language: isiZulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/317499 , vital:59947 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD142-05
- Description: Church hymn, accompanied by the Marimba and rattles.
- Full Text: false
- Date Issued: 1988
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Ursberg gw
- Language: isiZulu
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/317499 , vital:59947 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD142-05
- Description: Church hymn, accompanied by the Marimba and rattles.
- Full Text: false
- Date Issued: 1988
Chaminuka
- Tracey, Andrew, Dargie, Dave
- Authors: Tracey, Andrew , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Gobabis f-sx
- Language: Chishona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/308242 , vital:58896 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-48
- Description: Traditional Shona song accompanied by the mbira.
- Full Text: false
- Date Issued: 1988
- Authors: Tracey, Andrew , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Gobabis f-sx
- Language: Chishona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/308242 , vital:58896 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD116-48
- Description: Traditional Shona song accompanied by the mbira.
- Full Text: false
- Date Issued: 1988
Chisakoni galiwa ngavasadzi chisakoni galiwa ngavana
- Wilson Ravele, Tracey, Andrew T N
- Authors: Wilson Ravele , Tracey, Andrew T N
- Date: 1988
- Subjects: Venda (African people) -- South Africa , Folk music -- South Africa , Mbila , Sub-Saharan African music , Africa South Africa Venda f-sa
- Language: Venda
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/93342 , vital:30870 , International Library of African Music, Rhodes University, Grahamstown, South Africa , ATC024-06
- Description: Traditional song with mbila mutondo Venda xylophone accompaniment.
- Full Text: false
- Date Issued: 1988
- Authors: Wilson Ravele , Tracey, Andrew T N
- Date: 1988
- Subjects: Venda (African people) -- South Africa , Folk music -- South Africa , Mbila , Sub-Saharan African music , Africa South Africa Venda f-sa
- Language: Venda
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/93342 , vital:30870 , International Library of African Music, Rhodes University, Grahamstown, South Africa , ATC024-06
- Description: Traditional song with mbila mutondo Venda xylophone accompaniment.
- Full Text: false
- Date Issued: 1988
Christ ist erstanden
- Marimba Group, Composer Not Specified, Dargie, Dave
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Munich gw
- Language: Deutsch
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316761 , vital:59863 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD140-03
- Description: Church hymn, accompanied by the Marimba and rattles.
- Full Text: false
- Date Issued: 1988
- Authors: Marimba Group , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Music--Religious aspects , Field recordings , Africa, Sub-Saharan , Europe Germany Munich gw
- Language: Deutsch
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316761 , vital:59863 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD140-03
- Description: Church hymn, accompanied by the Marimba and rattles.
- Full Text: false
- Date Issued: 1988
Church Bell
- Church Choir, Composer Not Specified, Dargie, Dave
- Authors: Church Choir , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Gobabis f-sx
- Language: Afrikaans
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/309028 , vital:58989 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD117-52
- Description: Palm Sunday Mass Church Bell.
- Full Text: false
- Date Issued: 1988
- Authors: Church Choir , Composer Not Specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Gobabis f-sx
- Language: Afrikaans
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/309028 , vital:58989 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD117-52
- Description: Palm Sunday Mass Church Bell.
- Full Text: false
- Date Issued: 1988
Cleavage of the precursor coat protein of black beetle virus strain w17 in rabbit reticulocyte lysate
- Authors: Blackhurst, Diane Mary
- Date: 1988
- Subjects: Beetles , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3880 , http://hdl.handle.net/10962/d1001614
- Description: Black beetle virus (BBV) is a bipartite single-stranded RNA virus belonging to the family Nodaviridae. Its host range has been found to be limited to insects. RNA 1, the larger of the two RNA molecules, with a MW of 1,15 x 10⁶ and the smaller RNA 2 with a MW of 0,46 x 10⁶, are both packaged in the same virus particle. The two RNA molecules are translated separately, with RNA 1 coding for protein A of MW 105 x 10³ and RNA 2 coding for protein α of MW 47 x 10³. Protein α is the major capsid protein precursor, which during in vivo maturation is cleaved to form the coat protein β of MW 43 x 10³, and protein γ of MW 5 x 10³. Cell-free translation of BBV (strain W17) mRNA was carried out in rabbit reticulocyte lysates. Protein α was detectable between 0 and 30 minutes after RNA addition. A protein 'β', which was found to co-electrophorese on polyacrylamide gels with authentic β and which was immunoprecipitated by anti-BBV antiserum, was detectable after 30 minutes. Results of this work show that the formation of 'β' could be prevented by the addition of RNase to the lysate, indicating that intact RNA is necessary for α to β cleavage. Arresting protein synthesis by the addition of cycloheximide to the lysate mix did not inhibit the cleavage. The formation of β could also be prevented by cooling the lysate mix to 1°C. Cleavage of α to β still occurred when RNA 2, without the presence of RNA 1, was translated. Therefore the cleavage is not dependent on a translation product of RNA 1. Sedimentation of lysate on sucrose density gradients showed that α to β cleavage was not accompanied by assembly of BBV RNA and protein lnto a viral substructure as has been shown to occur with some viruses, for example certain picornaviruses. Serial dilution of lysate containing α showed that the level of β decreased with increasing dilution, indicating that the cleavage is not mediated by autocatalysis, but by some other unknown factor. Although much work has been carried out on black beetle virus, no work has been published to date concerning α to β cleavage as an indication of assembly in rabbit reticulocyte lysates. Results of these cell-free translation experiments thus indicate that BBV coat protein precursor α, in association with its messenger RNA 2, undergoes a maturation cleavage in the lysate to produce BBV coat protein β. In addition, this cleavage seems to occur without assembly into any intermediate viral structure
- Full Text:
- Date Issued: 1988
- Authors: Blackhurst, Diane Mary
- Date: 1988
- Subjects: Beetles , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3880 , http://hdl.handle.net/10962/d1001614
- Description: Black beetle virus (BBV) is a bipartite single-stranded RNA virus belonging to the family Nodaviridae. Its host range has been found to be limited to insects. RNA 1, the larger of the two RNA molecules, with a MW of 1,15 x 10⁶ and the smaller RNA 2 with a MW of 0,46 x 10⁶, are both packaged in the same virus particle. The two RNA molecules are translated separately, with RNA 1 coding for protein A of MW 105 x 10³ and RNA 2 coding for protein α of MW 47 x 10³. Protein α is the major capsid protein precursor, which during in vivo maturation is cleaved to form the coat protein β of MW 43 x 10³, and protein γ of MW 5 x 10³. Cell-free translation of BBV (strain W17) mRNA was carried out in rabbit reticulocyte lysates. Protein α was detectable between 0 and 30 minutes after RNA addition. A protein 'β', which was found to co-electrophorese on polyacrylamide gels with authentic β and which was immunoprecipitated by anti-BBV antiserum, was detectable after 30 minutes. Results of this work show that the formation of 'β' could be prevented by the addition of RNase to the lysate, indicating that intact RNA is necessary for α to β cleavage. Arresting protein synthesis by the addition of cycloheximide to the lysate mix did not inhibit the cleavage. The formation of β could also be prevented by cooling the lysate mix to 1°C. Cleavage of α to β still occurred when RNA 2, without the presence of RNA 1, was translated. Therefore the cleavage is not dependent on a translation product of RNA 1. Sedimentation of lysate on sucrose density gradients showed that α to β cleavage was not accompanied by assembly of BBV RNA and protein lnto a viral substructure as has been shown to occur with some viruses, for example certain picornaviruses. Serial dilution of lysate containing α showed that the level of β decreased with increasing dilution, indicating that the cleavage is not mediated by autocatalysis, but by some other unknown factor. Although much work has been carried out on black beetle virus, no work has been published to date concerning α to β cleavage as an indication of assembly in rabbit reticulocyte lysates. Results of these cell-free translation experiments thus indicate that BBV coat protein precursor α, in association with its messenger RNA 2, undergoes a maturation cleavage in the lysate to produce BBV coat protein β. In addition, this cleavage seems to occur without assembly into any intermediate viral structure
- Full Text:
- Date Issued: 1988
Collection Song
- Our Lady of Africa Congregation, Composer not specified, Dargie, Dave
- Authors: Our Lady of Africa Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307144 , vital:58768 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD114-24
- Description: Sung Mass at Our Lady of Africa with drums and rattle accompaniment.
- Full Text: false
- Date Issued: 1988
- Authors: Our Lady of Africa Congregation , Composer not specified , Dargie, Dave
- Date: 1988
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Namibia Ngwezi f-sx
- Language: Lozi
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/307144 , vital:58768 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD114-24
- Description: Sung Mass at Our Lady of Africa with drums and rattle accompaniment.
- Full Text: false
- Date Issued: 1988