VIVA Comrade Moss
- NUMSA
- Authors: NUMSA
- Date: 25 April
- Subjects: NUMSA
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/109640 , vital:33163 , SALHA 14b : NUMSA
- Description: The last 'Trial Update" reported on Comrade Moss’s case until mid-March. This report covers the period from that time up until 25th April.STADLER - "ANC EXPERT" Brigadier Stadler testified that the ANC had made certain calls to organisations within the country which, according to his "research", had been followed by organisations in the country. As examples he gave the ANC’s call for ungovernability, the formation of organs of people’s power and the destruction of town councils. During cross-examination Stadler conceded that he was not able to give examples of any direct link between the ANC and organisations inside the coutry but he said an inference could be drawn by looking at the ANC’s call and the behaviour of organisations inside the country.
- Full Text:
- Date Issued: 25 April
- Authors: NUMSA
- Date: 25 April
- Subjects: NUMSA
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/109640 , vital:33163 , SALHA 14b : NUMSA
- Description: The last 'Trial Update" reported on Comrade Moss’s case until mid-March. This report covers the period from that time up until 25th April.STADLER - "ANC EXPERT" Brigadier Stadler testified that the ANC had made certain calls to organisations within the country which, according to his "research", had been followed by organisations in the country. As examples he gave the ANC’s call for ungovernability, the formation of organs of people’s power and the destruction of town councils. During cross-examination Stadler conceded that he was not able to give examples of any direct link between the ANC and organisations inside the coutry but he said an inference could be drawn by looking at the ANC’s call and the behaviour of organisations inside the country.
- Full Text:
- Date Issued: 25 April
The prevalence of insufficient iodine intake in pregnancy in Africa: Protocol for a systematic review and meta-analysis
- Charles Bitamazire Businge, Benjamin Longo-Mbenza, Andre Pascal Kengne
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
- Date Issued: 22-8-2019
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
- Date Issued: 22-8-2019
Honorary Citation for the Conferment of Doctor of Laws honoris causa - Tandabantu Tennyson August
- Rhodes University, Martin, Tom
- Authors: Rhodes University , Martin, Tom
- Date: 2024-14-05
- Subjects: Uncatalogued
- Language: English
- Type: Speeches , Transcripts, speech , text
- Identifier: http://hdl.handle.net/10962/432591 , vital:72885
- Description: Rhodes University Honorary Doctorate Citation (5 April 2024) Tandabantu Tennyson August: 1980s. South Africa. In the Bantustans of the time, like the Transkei, the youth have little reason for hope. They have much reason for despair. Their choices seem limited to the politics of struggle or to crime, an attempt to gain some kind of advantage from the meagre and unacceptable options on offer. In those bleak times, what was needed was visionaries. People who could see beyond the current circumstances to a different future and imagine how best to prepare for it. Not as idealists, but as pragmatists. What would happen once circumstances change, once the struggle had succeeded? Would we be ready for it? What qualities would the youth need to have in order to both benefit from and participate in a new dispensation? One such visionary was Tandabantu Tennyson August, and it is my distinct pleasure to have the opportunity to tell you more about him. Born in 1948, Mr. August matriculated from Healdtown High School, before pursuing higher education at the University of Transkei, where obtained a Bachelor of Arts degree majoring in English and Geography. This was followed by Comparative Curriculum Studies at the University of South Africa, alongside obtaining teaching certificates from Lovedale and Cicira teacher training colleges, with majors in Physical Science, Mathematics, Science, and Mathematics. This solid educational foundation laid the groundwork for his impactful career in education. He taught at Wongalethu High, St Johns College and was deputy principal at Norwood, before taking up his first principalship, at Thanga High School in 1980. From 1984 to 1990 he was principal of Ndamase High School. During his tenure there, the matric pass rate never fell below 90%. From 1991 to 1995 he was principal Isivivane High School during which time the school was promoted from a primary to a high school. Following his distinguished service at schools, he worked at the Eastern Cape Department of Basic Education, first as a deputy chief education specialist (1996 – 2006) and then as chief education specialist (2006-2010). During that time, he played a pivotal role in establishing (in conjunction with Rhodes University) and being the provincial coordinator the Advanced Certificate in Education in English, followed by ACEs in mathematical literacy and life orientation. In 2016 he received a provincial teaching award for excellence. In 2017 he was awarded the Kade Asmal award for excellence in teaching. To this day, he remains an Executive member of the eastern cape Education advisory council. Quite a career. But there’s something I haven’t mentioned. He was also an accomplished rugby player of international standing. While at Lovedale College, he was selected to represent Border and then the South African African Rugby Board, playing England in Gqeberha in 1972, in a team that would later be known as the black springboks. He eventually received his green and gold springbok blazer as just recognition of his achievement. He incorporated his love of sport into his vision for the youth under his care, as part of his overriding mission of educating the whole person. He got everyone involved in sport, if not as a player, then as a supporter. Especially during his time as principal of Ndamase High School, I am led to believe, it was extremely intimidating for opposing teams to play them. Not only at Ndamase home games, but especially at their away games. There would be one bus for the team players, and six buses for the supporters. From 1986 – 1990: he was president of the Transkei schools` sports union (TSSU). But there’s yet another thing I haven’t mentioned. In 1981, just as his career as an educational leader was really taking off, he was involved in a terrible motor vehicle accident. It left him permanently disabled and, for much of his life, a wheelchair user. Rather than stopping him, it simply added another string to his bow, now as an advocate for the disabled. From 2012 – 2022: Chairperson Eastern Cape South African National Association For Special Education (SANASE): this organization cater for the education and wellbeing of learners with disabilities and special needs. In 2023, he was the national deputy president of the same organisation. From 2013 – to date: he is a Board Member on the National Council of & for persons with disabilities (NCPD). I had the pleasure of an extensive conversation with one of Mr August’s former pupils at Ndamase High, Professor Deyi Mbelani, the current director of Rhodes University’s Institute for the Study of Englishes in Africa. He told me many stories about Mr August’s leadership, that I only wish I had time to share with you now. I put it to Professor Mbelani that, having read about Mr August, the word that stood out for me was “dedication.” He agreed that “dedication” was the word, but that I must not leave out another: “vision”. It is Mr August’s status as a visionary that best defines him, he said. Going back over the above, I think he is right. Mr Chancellor I have the honour to request you to confer the degree of Doctor of Laws honoris causa on the Mr Tandabantu Tennyson August. *The above citation draws heavily on documents submitted as part of the honorary doctorate nomination process and others compiled by the Division of Communications and Advancement. - Tom Martin.
- Full Text:
- Date Issued: 2024-14-05
- Authors: Rhodes University , Martin, Tom
- Date: 2024-14-05
- Subjects: Uncatalogued
- Language: English
- Type: Speeches , Transcripts, speech , text
- Identifier: http://hdl.handle.net/10962/432591 , vital:72885
- Description: Rhodes University Honorary Doctorate Citation (5 April 2024) Tandabantu Tennyson August: 1980s. South Africa. In the Bantustans of the time, like the Transkei, the youth have little reason for hope. They have much reason for despair. Their choices seem limited to the politics of struggle or to crime, an attempt to gain some kind of advantage from the meagre and unacceptable options on offer. In those bleak times, what was needed was visionaries. People who could see beyond the current circumstances to a different future and imagine how best to prepare for it. Not as idealists, but as pragmatists. What would happen once circumstances change, once the struggle had succeeded? Would we be ready for it? What qualities would the youth need to have in order to both benefit from and participate in a new dispensation? One such visionary was Tandabantu Tennyson August, and it is my distinct pleasure to have the opportunity to tell you more about him. Born in 1948, Mr. August matriculated from Healdtown High School, before pursuing higher education at the University of Transkei, where obtained a Bachelor of Arts degree majoring in English and Geography. This was followed by Comparative Curriculum Studies at the University of South Africa, alongside obtaining teaching certificates from Lovedale and Cicira teacher training colleges, with majors in Physical Science, Mathematics, Science, and Mathematics. This solid educational foundation laid the groundwork for his impactful career in education. He taught at Wongalethu High, St Johns College and was deputy principal at Norwood, before taking up his first principalship, at Thanga High School in 1980. From 1984 to 1990 he was principal of Ndamase High School. During his tenure there, the matric pass rate never fell below 90%. From 1991 to 1995 he was principal Isivivane High School during which time the school was promoted from a primary to a high school. Following his distinguished service at schools, he worked at the Eastern Cape Department of Basic Education, first as a deputy chief education specialist (1996 – 2006) and then as chief education specialist (2006-2010). During that time, he played a pivotal role in establishing (in conjunction with Rhodes University) and being the provincial coordinator the Advanced Certificate in Education in English, followed by ACEs in mathematical literacy and life orientation. In 2016 he received a provincial teaching award for excellence. In 2017 he was awarded the Kade Asmal award for excellence in teaching. To this day, he remains an Executive member of the eastern cape Education advisory council. Quite a career. But there’s something I haven’t mentioned. He was also an accomplished rugby player of international standing. While at Lovedale College, he was selected to represent Border and then the South African African Rugby Board, playing England in Gqeberha in 1972, in a team that would later be known as the black springboks. He eventually received his green and gold springbok blazer as just recognition of his achievement. He incorporated his love of sport into his vision for the youth under his care, as part of his overriding mission of educating the whole person. He got everyone involved in sport, if not as a player, then as a supporter. Especially during his time as principal of Ndamase High School, I am led to believe, it was extremely intimidating for opposing teams to play them. Not only at Ndamase home games, but especially at their away games. There would be one bus for the team players, and six buses for the supporters. From 1986 – 1990: he was president of the Transkei schools` sports union (TSSU). But there’s yet another thing I haven’t mentioned. In 1981, just as his career as an educational leader was really taking off, he was involved in a terrible motor vehicle accident. It left him permanently disabled and, for much of his life, a wheelchair user. Rather than stopping him, it simply added another string to his bow, now as an advocate for the disabled. From 2012 – 2022: Chairperson Eastern Cape South African National Association For Special Education (SANASE): this organization cater for the education and wellbeing of learners with disabilities and special needs. In 2023, he was the national deputy president of the same organisation. From 2013 – to date: he is a Board Member on the National Council of & for persons with disabilities (NCPD). I had the pleasure of an extensive conversation with one of Mr August’s former pupils at Ndamase High, Professor Deyi Mbelani, the current director of Rhodes University’s Institute for the Study of Englishes in Africa. He told me many stories about Mr August’s leadership, that I only wish I had time to share with you now. I put it to Professor Mbelani that, having read about Mr August, the word that stood out for me was “dedication.” He agreed that “dedication” was the word, but that I must not leave out another: “vision”. It is Mr August’s status as a visionary that best defines him, he said. Going back over the above, I think he is right. Mr Chancellor I have the honour to request you to confer the degree of Doctor of Laws honoris causa on the Mr Tandabantu Tennyson August. *The above citation draws heavily on documents submitted as part of the honorary doctorate nomination process and others compiled by the Division of Communications and Advancement. - Tom Martin.
- Full Text:
- Date Issued: 2024-14-05
A MIGHTEE Investigation of radio quiet AGN
- Authors: Namane, Neo
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435367 , vital:73151
- Description: This study is aimed at being an overview and investigation of the behaviour and morphology of radio quiet (RQ) active galactic nuclei (AGN) in the radio and optical/near-infrared (NIR) bands. It is hoped that a concise description of the relation that exists between accretion activity and star formation (SF) will be achieved through utilization of multiwavelength astronomy analysis. This analysis includes the processing of data acquired from the MeerKAT International GHz Tiered Extragalactic Exploration survey (MIGHTEE), the Southern African Large Telescope (SALT), the Hyper Suprime Camera (HSC) mounted on the Subaru telescope and the VISTA telescope. In this thesis, several targets were observed using SALT spectroscopy, but a redshift of only one of them was obtained. Lastly, it was established that for the sample of RQ AGN studied, the AGN contribute a large fraction of the radio emission observed. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Namane, Neo
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435367 , vital:73151
- Description: This study is aimed at being an overview and investigation of the behaviour and morphology of radio quiet (RQ) active galactic nuclei (AGN) in the radio and optical/near-infrared (NIR) bands. It is hoped that a concise description of the relation that exists between accretion activity and star formation (SF) will be achieved through utilization of multiwavelength astronomy analysis. This analysis includes the processing of data acquired from the MeerKAT International GHz Tiered Extragalactic Exploration survey (MIGHTEE), the Southern African Large Telescope (SALT), the Hyper Suprime Camera (HSC) mounted on the Subaru telescope and the VISTA telescope. In this thesis, several targets were observed using SALT spectroscopy, but a redshift of only one of them was obtained. Lastly, it was established that for the sample of RQ AGN studied, the AGN contribute a large fraction of the radio emission observed. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-04-04
Acacia dealbata invasion and clearing in Upper Tsitsana communal areas of South Africa: human perceptions and ecological impacts
- Authors: Coka, Nwabisa
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434876 , vital:73113
- Description: The number of invasive alien plants in South Africa and globally continue to increase mostly due to increased human movement and climate change. It is estimated that approximately 10 000 hectares of South Africa’s terrestrial land is invaded by invasive alien species. Recent studies have reported that invasion by invasive alien plants cost South Africa billions of Rands every year through loss of water and ecosystem services. Although much has been done to understanding invasion trends, patterns, and processes in South Africa, little is knowns regarding human benefits linked to alien plant invasion as well as human perceptions regarding management interventions, particularly clearing by the national Working for Water programme. This thesis took a socio-ecological approach to assess (i) local people perceptions and knowledge of Acacia dealbata invasion and clearing, and (ii) extent of grazing grass diversity and composition recovery following A. dealbata clearing. To answer the above-mentioned objectives, both qualitative (face-to-face interviews) and quantitative (vegetation surveys) methods were used in grasslands located in upper Tsitsana catchment, South Africa. Results from the 165 interviews conducted in six villages show that most villagers are aware of A. dealbata in the area, but do not know that it is an invasive alien plant. Respondents accrue several benefits from the plant that include firewood (98% across all villages), construction pools (34% across all villages), and shade (3% across all villages). Respondents identified several costs associated with A. dealbata invasion, such as roots damage to houses (37% across all villages), hindering growth of crops (15% across all villages), and takes up yard space (29% across all villages). Most of the villages ranked the effects of A. dealbata on grazing as highly severe. Villages indicated that the Working for Water clearing programme presents them with socio-economic benefits such as employment and money. Some villagers noted that the clearing of A. dealbata in the area restores grazing grass (62% across all villages) and water (32% across all villages). Based on these interview survey results, the study concludes that A. dealbata has both costs and benefits that support local people’s livelihoods; therefore, its management should consider views for local users. Grass surveys conducted in 1 m2 quadrats that were replicated 15 times across four paired A. dealbata cleared and grassland sites showed that the removal of A. dealbata facilitates the recovery of native grasses. Of the 14 different grazable grass species that were identified across all quadrats only one (Bulbostylis sp.) is not palatable to livestock. Although grass cover was significantly (p < 0.05) higher in the grasslands than the cleared patches, grass richness was significantly (p < 0.05) higher in the cleared patches an indication that recovery of different grasses is taking place. Based on these vegetation survey results; the study concludes that recovery of grazing grasses after A. dealbata clearing four years ago is following a positive trajectory as the cleared sites are looking like the grassland sites. Overall, the study recommends that management of A. dealbata in Tsitsana landscape requires a socio-ecological approach to understand user benefits whilst at the same time developing measure to control the plant. The study has provided evidence that A. dealbata clearing facilitates passive restoration of grazing grass and local villages are aware of these restoration benefits. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Coka, Nwabisa
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434876 , vital:73113
- Description: The number of invasive alien plants in South Africa and globally continue to increase mostly due to increased human movement and climate change. It is estimated that approximately 10 000 hectares of South Africa’s terrestrial land is invaded by invasive alien species. Recent studies have reported that invasion by invasive alien plants cost South Africa billions of Rands every year through loss of water and ecosystem services. Although much has been done to understanding invasion trends, patterns, and processes in South Africa, little is knowns regarding human benefits linked to alien plant invasion as well as human perceptions regarding management interventions, particularly clearing by the national Working for Water programme. This thesis took a socio-ecological approach to assess (i) local people perceptions and knowledge of Acacia dealbata invasion and clearing, and (ii) extent of grazing grass diversity and composition recovery following A. dealbata clearing. To answer the above-mentioned objectives, both qualitative (face-to-face interviews) and quantitative (vegetation surveys) methods were used in grasslands located in upper Tsitsana catchment, South Africa. Results from the 165 interviews conducted in six villages show that most villagers are aware of A. dealbata in the area, but do not know that it is an invasive alien plant. Respondents accrue several benefits from the plant that include firewood (98% across all villages), construction pools (34% across all villages), and shade (3% across all villages). Respondents identified several costs associated with A. dealbata invasion, such as roots damage to houses (37% across all villages), hindering growth of crops (15% across all villages), and takes up yard space (29% across all villages). Most of the villages ranked the effects of A. dealbata on grazing as highly severe. Villages indicated that the Working for Water clearing programme presents them with socio-economic benefits such as employment and money. Some villagers noted that the clearing of A. dealbata in the area restores grazing grass (62% across all villages) and water (32% across all villages). Based on these interview survey results, the study concludes that A. dealbata has both costs and benefits that support local people’s livelihoods; therefore, its management should consider views for local users. Grass surveys conducted in 1 m2 quadrats that were replicated 15 times across four paired A. dealbata cleared and grassland sites showed that the removal of A. dealbata facilitates the recovery of native grasses. Of the 14 different grazable grass species that were identified across all quadrats only one (Bulbostylis sp.) is not palatable to livestock. Although grass cover was significantly (p < 0.05) higher in the grasslands than the cleared patches, grass richness was significantly (p < 0.05) higher in the cleared patches an indication that recovery of different grasses is taking place. Based on these vegetation survey results; the study concludes that recovery of grazing grasses after A. dealbata clearing four years ago is following a positive trajectory as the cleared sites are looking like the grassland sites. Overall, the study recommends that management of A. dealbata in Tsitsana landscape requires a socio-ecological approach to understand user benefits whilst at the same time developing measure to control the plant. The study has provided evidence that A. dealbata clearing facilitates passive restoration of grazing grass and local villages are aware of these restoration benefits. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2024
- Full Text:
- Date Issued: 2024-04-04
Aptamer-based biosensor for prostate specific antigen detection using cobalt phthalocyanine-exfoliated graphite composites
- Authors: Benise, Emihle
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434850 , vital:73110
- Description: The work focuses on the development of biosensors and their use for the detection of prostate specific antigen (PSA). Four cobalt phthalocyanines (CoPcs) complexes: (1) cobalt tetra pyridyloxy phthalocyanine, (2) cobalt tetra acetamidophenoxy phthalocyanine, (3) cobalt tris(acetamidophenoxy) mono benzoic acid phthalocyanine, and (4) cobalt tris(acetamidophenoxy) mono propionic acid phthalocyanine, an exfoliated graphite (EG), and aptamer are used to make probes for PSA detection. Each complex is π-π stacked onto the EG to form EG-CoPc(π-π) hybrid which was used to modify a glassy carbon electrode (GCE). EG and CoPc were also used to modify the GCE sequential (seq) with CoPc on top to give GCE-EG-CoPc(seq). For the detection PSA, PSA specific aptamer was either sequential added or covalently linked to complexes 3 and 4 on the modified electrodes and was only sequentially added onto complexes 1 and 2 modified electrodes. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were the techniques used for the detection of PSA. The electrodes were found to be selective in bovine serum albumin, glucose and cysteine and stable when 50 DPV scans were run. Electrodes gave good % recovery when human serum was spiked with different PSA concentrations. , Thesis (MSc) -- Faculty of Science, Chemistry, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Benise, Emihle
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434850 , vital:73110
- Description: The work focuses on the development of biosensors and their use for the detection of prostate specific antigen (PSA). Four cobalt phthalocyanines (CoPcs) complexes: (1) cobalt tetra pyridyloxy phthalocyanine, (2) cobalt tetra acetamidophenoxy phthalocyanine, (3) cobalt tris(acetamidophenoxy) mono benzoic acid phthalocyanine, and (4) cobalt tris(acetamidophenoxy) mono propionic acid phthalocyanine, an exfoliated graphite (EG), and aptamer are used to make probes for PSA detection. Each complex is π-π stacked onto the EG to form EG-CoPc(π-π) hybrid which was used to modify a glassy carbon electrode (GCE). EG and CoPc were also used to modify the GCE sequential (seq) with CoPc on top to give GCE-EG-CoPc(seq). For the detection PSA, PSA specific aptamer was either sequential added or covalently linked to complexes 3 and 4 on the modified electrodes and was only sequentially added onto complexes 1 and 2 modified electrodes. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were the techniques used for the detection of PSA. The electrodes were found to be selective in bovine serum albumin, glucose and cysteine and stable when 50 DPV scans were run. Electrodes gave good % recovery when human serum was spiked with different PSA concentrations. , Thesis (MSc) -- Faculty of Science, Chemistry, 2024
- Full Text:
- Date Issued: 2024-04-04
Computational design for genome reduction of Lactococcus lactis towards microbial chassis development
- Authors: Hamese, Saltiel
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435321 , vital:73147
- Description: Access restricted. Expected release date 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Hamese, Saltiel
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435321 , vital:73147
- Description: Access restricted. Expected release date 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
- Full Text:
- Date Issued: 2024-04-04
Developing a community of practice to promote the use of biological control in the integrated management of Prosopis in South Africa
- Authors: Van Staden, Gretha
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434998 , vital:73122
- Description: Prosopis spp. are non-native species present in the arid parts of southern Africa. These trees originated in the Americas and were first introduced as a source of fodder, shade, and wood, but have become invasive, negatively impacting local biodiversity, and disrupting the delivery of ecosystem services. Some species of Prosopis hybridise freely, complicating identification and subsequent control. The control of Prosopis in the Northern Cape Province of South Africa is still contentious, because of the perceived benefits to some landowners. The effectiveness of biological control agents to control the spread of Prosopis has been quantified, more damaging agents have been considered in recent years because of the continued increase in Prosopis density. Research into the establishment of a Community of Practice (CoP) to address the differences in perceptions regarding the control of Prosopis in the Northern Cape Province is considered an appropriate starting point to conceptualise the challenges to the successful integrated management of Prosopis. As farmers are the main stakeholders involved with Prosopis control, understanding the role of farmers and the functioning of farming enterprises in the Northern Cape is imperative for the development of both a CoP as well as the appropriate management of Prosopis. To foster engagement with as many stakeholders as possible, workshops dealing with biological control as part on an integrated approach to the control of Prosopis were held in towns in the Northern Cape Province: Groblershoop, Brandvlei, Kenhardt, Upington, Prieska and Williston. Some of the main concerns of the landusers include the host specificity of the released biocontrol agents, as well as increased transparency and communication. This is especially in regards to the research on present and future biological control agents that will possibly improve the perceptions of stakeholders. The mechanical harvesting and use of biomass as a control method was supported, especially where farmers were removing Prosopis from their properties, leaving large amounts of biomass in the veld. Continued research into Prosopis in the Northern Cape needs to consider the possibility of secondary invasions, especially in areas where native bush encroachment is already a concern. The impact of clearing in terms of natural grazing and animal improvement needs to be quantified, to conceptualise the importance of maintaining better veld quality versus using Prosopis for fodder. Biological control targeting not only the reproductive output of the trees, but also the standing biomass has support from the majority of the land users in the Northern Cape Province following these workshops and this study shows the importance of a socio-ecological approach to the control of landscape scale invasion. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Van Staden, Gretha
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434998 , vital:73122
- Description: Prosopis spp. are non-native species present in the arid parts of southern Africa. These trees originated in the Americas and were first introduced as a source of fodder, shade, and wood, but have become invasive, negatively impacting local biodiversity, and disrupting the delivery of ecosystem services. Some species of Prosopis hybridise freely, complicating identification and subsequent control. The control of Prosopis in the Northern Cape Province of South Africa is still contentious, because of the perceived benefits to some landowners. The effectiveness of biological control agents to control the spread of Prosopis has been quantified, more damaging agents have been considered in recent years because of the continued increase in Prosopis density. Research into the establishment of a Community of Practice (CoP) to address the differences in perceptions regarding the control of Prosopis in the Northern Cape Province is considered an appropriate starting point to conceptualise the challenges to the successful integrated management of Prosopis. As farmers are the main stakeholders involved with Prosopis control, understanding the role of farmers and the functioning of farming enterprises in the Northern Cape is imperative for the development of both a CoP as well as the appropriate management of Prosopis. To foster engagement with as many stakeholders as possible, workshops dealing with biological control as part on an integrated approach to the control of Prosopis were held in towns in the Northern Cape Province: Groblershoop, Brandvlei, Kenhardt, Upington, Prieska and Williston. Some of the main concerns of the landusers include the host specificity of the released biocontrol agents, as well as increased transparency and communication. This is especially in regards to the research on present and future biological control agents that will possibly improve the perceptions of stakeholders. The mechanical harvesting and use of biomass as a control method was supported, especially where farmers were removing Prosopis from their properties, leaving large amounts of biomass in the veld. Continued research into Prosopis in the Northern Cape needs to consider the possibility of secondary invasions, especially in areas where native bush encroachment is already a concern. The impact of clearing in terms of natural grazing and animal improvement needs to be quantified, to conceptualise the importance of maintaining better veld quality versus using Prosopis for fodder. Biological control targeting not only the reproductive output of the trees, but also the standing biomass has support from the majority of the land users in the Northern Cape Province following these workshops and this study shows the importance of a socio-ecological approach to the control of landscape scale invasion. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
Elucidation of the roles of the carbonic anhydrase enzymes, CanA & CanB, in the physiology of Mycobacterium smegmatis
- Authors: Jackson, Gabriella Teresa
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435299 , vital:73145
- Description: The bacterial pathogen Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB) and one of the leading infectious causes of death globally. The success of Mtb as a pathogen depends on its ability to detect and respond to a variety of physical and chemical stresses it encounters during infection of its human host. These environmental stresses include shifts in temperature, oxygen concentration, osmolarity and nutrient availability. Mtb is, in addition, exposed to changes in pH and CO2 concentration in the intracellular and extracellular environments it inhabits, which the bacterium has to adapt to in order to ensure its growth, survival and/or persistence during infection. Carbonic anhydrases (CAs) are a widely distributed family of enzymes that catalyse the reversible hydration of carbon dioxide (CO2) to bicarbonate (HCO3−) in the reaction: CO2 + H2O ⇄ HCO3− + H+. In microbes, CA activity is important for the activity of enzymes involved in carbon fixation as well as for maintaining pH homeostasis. Mtb is known to express three CAs, encoded by the Rv3588c, Rv1284 and Rv3273 genes (canA, canB and canC, respectively). The role(s) of these CA enzymes in the physiology of Mtb and other mycobacterial species, such as Mycobacterium smegmatis (Msm), has not been elucidated to date. To gain insights into the function of the CanA and CanB enzymes in mycobacterial species, we generated both canA and canB knockdown (KD) and knockout (KO) mutants in the fast-growing mycobacterial species, Msm, and analysed their growth phenotypes under several growth conditions where CA activity is known to be required. Notably, Msm lacks the CanC homologue, which makes it an ideal surrogate to focus on CanA and CanB. The Msm canA KD mutant was found to display a growth defect following anhydrotetracycline (ATc)-mediated gene silencing at atmospheric (low) CO2 concentrations [~0.035% CO2 (v/v)]. The growth defect could be rescued by incubating cells at physiological (high) CO2 concentrations [~5% CO2 (v/v)] or by supplementing the growth media with either HCO3− or the metabolic end-products of certain HCO3−-dependent-carboxylase enzymes at low CO2 concentrations. The ability of these compounds to rescue the growth of the canA KD mutants was, however, dependent on the extent of ATc-mediated gene silencing, suggesting that the canA gene is required for Msm growth at both low and high CO2 concentrations. This was confirmed by our findings that canA could only be genetically inactivated when a second copy of the gene was provided on the chromosome in trans, regardless of the CO2 concentration used. In contrast to our observations for canA, no differences in the growth phenotypes of the Msm wild type (WT) and canB KD or knockout (KO) mutant strains were observed following silencing or inactivation of the canB gene at either low or high CO2 concentrations or different pH values. These observations suggest that, in contrast to canA, the canB gene is dispensable for the growth of Msm under standard laboratory growth conditions. The canB KO mutant strain, nevertheless, displayed a slight decrease in its ability to form biofilms when compared to the WT strain, which could be restored by genetic complementation. CanB activity may, therefore, be required to promote bacterial growth and/or survival under biofilm conditions where CO2 diffusion into cells is limited, a phenomenon that has recently been observed in other microbes. Further studies are required to confirm the role of CanB in biofilm formation and to determine how the different CA enzymes cooperate to promote the growth and survival of mycobacterial species in the various environments they are known to inhabit. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Jackson, Gabriella Teresa
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435299 , vital:73145
- Description: The bacterial pathogen Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB) and one of the leading infectious causes of death globally. The success of Mtb as a pathogen depends on its ability to detect and respond to a variety of physical and chemical stresses it encounters during infection of its human host. These environmental stresses include shifts in temperature, oxygen concentration, osmolarity and nutrient availability. Mtb is, in addition, exposed to changes in pH and CO2 concentration in the intracellular and extracellular environments it inhabits, which the bacterium has to adapt to in order to ensure its growth, survival and/or persistence during infection. Carbonic anhydrases (CAs) are a widely distributed family of enzymes that catalyse the reversible hydration of carbon dioxide (CO2) to bicarbonate (HCO3−) in the reaction: CO2 + H2O ⇄ HCO3− + H+. In microbes, CA activity is important for the activity of enzymes involved in carbon fixation as well as for maintaining pH homeostasis. Mtb is known to express three CAs, encoded by the Rv3588c, Rv1284 and Rv3273 genes (canA, canB and canC, respectively). The role(s) of these CA enzymes in the physiology of Mtb and other mycobacterial species, such as Mycobacterium smegmatis (Msm), has not been elucidated to date. To gain insights into the function of the CanA and CanB enzymes in mycobacterial species, we generated both canA and canB knockdown (KD) and knockout (KO) mutants in the fast-growing mycobacterial species, Msm, and analysed their growth phenotypes under several growth conditions where CA activity is known to be required. Notably, Msm lacks the CanC homologue, which makes it an ideal surrogate to focus on CanA and CanB. The Msm canA KD mutant was found to display a growth defect following anhydrotetracycline (ATc)-mediated gene silencing at atmospheric (low) CO2 concentrations [~0.035% CO2 (v/v)]. The growth defect could be rescued by incubating cells at physiological (high) CO2 concentrations [~5% CO2 (v/v)] or by supplementing the growth media with either HCO3− or the metabolic end-products of certain HCO3−-dependent-carboxylase enzymes at low CO2 concentrations. The ability of these compounds to rescue the growth of the canA KD mutants was, however, dependent on the extent of ATc-mediated gene silencing, suggesting that the canA gene is required for Msm growth at both low and high CO2 concentrations. This was confirmed by our findings that canA could only be genetically inactivated when a second copy of the gene was provided on the chromosome in trans, regardless of the CO2 concentration used. In contrast to our observations for canA, no differences in the growth phenotypes of the Msm wild type (WT) and canB KD or knockout (KO) mutant strains were observed following silencing or inactivation of the canB gene at either low or high CO2 concentrations or different pH values. These observations suggest that, in contrast to canA, the canB gene is dispensable for the growth of Msm under standard laboratory growth conditions. The canB KO mutant strain, nevertheless, displayed a slight decrease in its ability to form biofilms when compared to the WT strain, which could be restored by genetic complementation. CanB activity may, therefore, be required to promote bacterial growth and/or survival under biofilm conditions where CO2 diffusion into cells is limited, a phenomenon that has recently been observed in other microbes. Further studies are required to confirm the role of CanB in biofilm formation and to determine how the different CA enzymes cooperate to promote the growth and survival of mycobacterial species in the various environments they are known to inhabit. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2024
- Full Text:
- Date Issued: 2024-04-04
Functional significance of mixed-species groups for zebra (Equus quagga) in savanna habitats
- Corry-Roberts, Carys Alexandra
- Authors: Corry-Roberts, Carys Alexandra
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434925 , vital:73116
- Description: Globally, herbivore populations are facing decline, which threatens their crucial role within the ecosystems they inhabit. Herbivores are essential to ecosystem structure and function because they act as prey for higher trophic levels, cycle soil nutrients, and alter vegetation structure. Understanding the drivers that shape herbivore habitat use and selection are therefore critical for making informed conservation and management decisions. Like all prey species, herbivores must balance a trade-off between acquiring resources and avoiding predation which is influenced by a number of species-specific intrinsic traits and various ecological drivers. Herbivores can also track risk over space and time, and certain habitat structures and landscape features can influence risk perception; this is termed the landscape of fear. To mitigate the risk-reward trade-off, herbivores employ a suite of behavioural responses. Group formation is likely the most well-documented of these responses. However, grouping results in the information-competition trade-off, wherein group members may obtain valuable information regarding the location of resources and/or the risk of predation, but may suffer from increased competition, especially among larger group sizes. Mixed-species groups—an assemblage of two or more species in close spatial association—have the potential to mitigate some of the competitive costs of larger conspecific groups and enhance the anti-predator and/or foraging benefits of grouping. African savannas provide a particularly useful natural laboratory to study mixed-species groups, due to the high diversity of prey species and habitat heterogeneity present. Previous studies have investigated the mixed-species effect within these systems, but have yet to quantify some of the identity-dependent costs and benefits associated with mixed-species herding. Thus, the overall aim of my study was to quantify the functional significance of mixed-species groups for zebra (Equus quagga) herding with two of their most common herding partners, impala (Aepyceros melampus) and wildebeest (Connochaetes taurinus). My study was conducted at multiple spatial scales in the southern portion of the Kruger National Park, South Africa between 2021 and 2022. I found that mixed-species herding altered zebra landscape use, resulting in a quantifiable intensification of habitat use compared to when they herd with conspecifics. I did not observe significant habitat expansion for zebra in mixed-species herds compared to single-species herds, likely because the anti-predator benefits of mixed-species herding convey enhanced foraging opportunities in already-safe habitats. Zebra foraging with impala achieved greater foraging success and experienced lower levels of competition, compared to zebra foraging with wildebeest. However, compared to foraging with conspecifics, the levelled landscape of fear zebra experienced when foraging with wildebeest resulted in increased foraging success across the landscape. My findings highlight the context-dependency of the costs and benefits associated with different herding partners for zebra, and suggest that in mixed-species herds with wildebeest, zebra experience increased potential fitness benefits compared to herding with impala or conspecifics. Overall, the findings of my study suggest that mixed-species herding is an important tool in the suite of anti-predator behaviours employed by prey species, and one with significant implications for survivorship, habitat use, and ultimately, community level interactions. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Corry-Roberts, Carys Alexandra
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434925 , vital:73116
- Description: Globally, herbivore populations are facing decline, which threatens their crucial role within the ecosystems they inhabit. Herbivores are essential to ecosystem structure and function because they act as prey for higher trophic levels, cycle soil nutrients, and alter vegetation structure. Understanding the drivers that shape herbivore habitat use and selection are therefore critical for making informed conservation and management decisions. Like all prey species, herbivores must balance a trade-off between acquiring resources and avoiding predation which is influenced by a number of species-specific intrinsic traits and various ecological drivers. Herbivores can also track risk over space and time, and certain habitat structures and landscape features can influence risk perception; this is termed the landscape of fear. To mitigate the risk-reward trade-off, herbivores employ a suite of behavioural responses. Group formation is likely the most well-documented of these responses. However, grouping results in the information-competition trade-off, wherein group members may obtain valuable information regarding the location of resources and/or the risk of predation, but may suffer from increased competition, especially among larger group sizes. Mixed-species groups—an assemblage of two or more species in close spatial association—have the potential to mitigate some of the competitive costs of larger conspecific groups and enhance the anti-predator and/or foraging benefits of grouping. African savannas provide a particularly useful natural laboratory to study mixed-species groups, due to the high diversity of prey species and habitat heterogeneity present. Previous studies have investigated the mixed-species effect within these systems, but have yet to quantify some of the identity-dependent costs and benefits associated with mixed-species herding. Thus, the overall aim of my study was to quantify the functional significance of mixed-species groups for zebra (Equus quagga) herding with two of their most common herding partners, impala (Aepyceros melampus) and wildebeest (Connochaetes taurinus). My study was conducted at multiple spatial scales in the southern portion of the Kruger National Park, South Africa between 2021 and 2022. I found that mixed-species herding altered zebra landscape use, resulting in a quantifiable intensification of habitat use compared to when they herd with conspecifics. I did not observe significant habitat expansion for zebra in mixed-species herds compared to single-species herds, likely because the anti-predator benefits of mixed-species herding convey enhanced foraging opportunities in already-safe habitats. Zebra foraging with impala achieved greater foraging success and experienced lower levels of competition, compared to zebra foraging with wildebeest. However, compared to foraging with conspecifics, the levelled landscape of fear zebra experienced when foraging with wildebeest resulted in increased foraging success across the landscape. My findings highlight the context-dependency of the costs and benefits associated with different herding partners for zebra, and suggest that in mixed-species herds with wildebeest, zebra experience increased potential fitness benefits compared to herding with impala or conspecifics. Overall, the findings of my study suggest that mixed-species herding is an important tool in the suite of anti-predator behaviours employed by prey species, and one with significant implications for survivorship, habitat use, and ultimately, community level interactions. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
High-altitude temporary systems and macroinvertebrate composition in La Nina cycle, Maloti-Drakensberg
- Authors: Moyo, Sibusisiwe
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434963 , vital:73119
- Description: The Maloti-Drakensberg Mountain is an important water catchment area for South Africa and Lesotho. It is rich in biodiversity in terms of endemic flora and fauna and has a high density of temporary wetlands in the form of Afromontane tarns and rock pools. There is, however, limited information on the distribution of macroinvertebrates in these ecosystems and environmental factors that drives their composition. To that end, this study investigated macroinvertebrate diversity and the effect of environmental factors on community assemblage in the Maloti-Drakensberg Mountain temporary wetlands, specifically tarns and rock pools. The main aims were to describe the abundance, diversity, and distribution of macroinvertebrates in temporary wetlands in the Maloti-Drakensberg Mountain. Furthermore, it aimed to investigate the effect of environmental variables on habitat structuring, spatial variations, and macroinvertebrate community composition in tarns and rockpools in the Maloti-Drakensberg Mountain. Seventy temporary wetlands (29 tarns and 41 rock pools) were surveyed over a once-off sampling (3 March – 7 April 2022). The wetlands were from 3 provinces including Eastern Cape, Free State, and KwaZulu-Natal. 15278 individual macroinvertebrates from 13 orders, 35 families, and 46 genera were collected and identified. Species accumulation curves incorporating both tarns and rock pools showed that observed results were significantly lower than Chao 1 and Chao 2 estimates meaning that the sampled sites were not enough to provide a comprehensive picture of the biodiversity in the region. This was also observed for rock pools when the temporary wetlands were analysed separately, however, the Chao 1 and Chao 2 values for tarns were almost equal to the observed values implying that sampled sites were enough to give a detailed picture of macroinvertebrate composition in tarns. Tarns and rock pools were significantly different in terms of species richness and Shannon-Wiener diversity index but showed no significant differences for relative abundance and Pielou’s evenness. Of the 35 families identified, 19 were considered core taxa with Dytiscidae and Corixidae being the most dominant. Additionally, there was moderate Nestedness metric based on Overlap and Decreasing Fill (NODF) value of 33.05369, with 32 out of 35 families being idiosyncratic. Predators were the dominant feeding group with omnivores being the least common. Non-Metric multi-Dimensional Scaling (NMDS) and PERMANOVA results showed that tarns and rock pools supported distinctly different macroinvertebrate communities per wetland type and location respectively. Physicochemically, tarns and rock pools were significantly different in terms of water temperature, depth, area, and chlorophyll-a concentration with tarns exhibiting higher values. Temporary wetlands in the sampled localities differed in terms of pH, EC, DO, water temperature, altitude, and phosphate. Principal Component Analysis showed that tarns were positively associated with area and depth whereas rock pools with phosphate. Akaike’s Information Criterion (AICc) selection showed that area, pH, and water temperature were significantly influencing community assemblage in temporary wetlands. Furthermore, area significantly influenced all four measured biodiversity indices: relative abundance, species richness, Pielou’s evenness and Shannon-Wiener Index, with water temperature also influencing relative abundance and altitude, species richness. Overall, the species composition showed that the sites were sampled later in the hydroperiod as evidenced by the dominance of active dispersers. The extended distribution of Prosthetops gladiator in the Eastern Cape highlights the need for extensive surveys to provide valuable insight into the distribution patterns of macroinvertebrates in the region. Furthermore, the interplay between physicochemical variables and biodiversity indices emphasized area, water temperature and pH as significant factors. However, their limited explanatory power suggests that obscure factors also influence the diversity cementing the need for further exploration of these enigmatic systems. Taxonomic challenges, especially regarding large branchiopods, hindered precise species distribution and conservation status determination. This study is the first to provide a comprehensive list of macroinvertebrates from temporary wetlands in the Maloti-Drakensberg Mountain. These results establish a valuable baseline for future biological monitoring studies, particularly in assessing species composition and responses to anthropogenic activities. It is a timeous study considering the sensitivity of these systems to climate change as they act as sentinel sites hence the need for foundational data on which to make future projections. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Moyo, Sibusisiwe
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434963 , vital:73119
- Description: The Maloti-Drakensberg Mountain is an important water catchment area for South Africa and Lesotho. It is rich in biodiversity in terms of endemic flora and fauna and has a high density of temporary wetlands in the form of Afromontane tarns and rock pools. There is, however, limited information on the distribution of macroinvertebrates in these ecosystems and environmental factors that drives their composition. To that end, this study investigated macroinvertebrate diversity and the effect of environmental factors on community assemblage in the Maloti-Drakensberg Mountain temporary wetlands, specifically tarns and rock pools. The main aims were to describe the abundance, diversity, and distribution of macroinvertebrates in temporary wetlands in the Maloti-Drakensberg Mountain. Furthermore, it aimed to investigate the effect of environmental variables on habitat structuring, spatial variations, and macroinvertebrate community composition in tarns and rockpools in the Maloti-Drakensberg Mountain. Seventy temporary wetlands (29 tarns and 41 rock pools) were surveyed over a once-off sampling (3 March – 7 April 2022). The wetlands were from 3 provinces including Eastern Cape, Free State, and KwaZulu-Natal. 15278 individual macroinvertebrates from 13 orders, 35 families, and 46 genera were collected and identified. Species accumulation curves incorporating both tarns and rock pools showed that observed results were significantly lower than Chao 1 and Chao 2 estimates meaning that the sampled sites were not enough to provide a comprehensive picture of the biodiversity in the region. This was also observed for rock pools when the temporary wetlands were analysed separately, however, the Chao 1 and Chao 2 values for tarns were almost equal to the observed values implying that sampled sites were enough to give a detailed picture of macroinvertebrate composition in tarns. Tarns and rock pools were significantly different in terms of species richness and Shannon-Wiener diversity index but showed no significant differences for relative abundance and Pielou’s evenness. Of the 35 families identified, 19 were considered core taxa with Dytiscidae and Corixidae being the most dominant. Additionally, there was moderate Nestedness metric based on Overlap and Decreasing Fill (NODF) value of 33.05369, with 32 out of 35 families being idiosyncratic. Predators were the dominant feeding group with omnivores being the least common. Non-Metric multi-Dimensional Scaling (NMDS) and PERMANOVA results showed that tarns and rock pools supported distinctly different macroinvertebrate communities per wetland type and location respectively. Physicochemically, tarns and rock pools were significantly different in terms of water temperature, depth, area, and chlorophyll-a concentration with tarns exhibiting higher values. Temporary wetlands in the sampled localities differed in terms of pH, EC, DO, water temperature, altitude, and phosphate. Principal Component Analysis showed that tarns were positively associated with area and depth whereas rock pools with phosphate. Akaike’s Information Criterion (AICc) selection showed that area, pH, and water temperature were significantly influencing community assemblage in temporary wetlands. Furthermore, area significantly influenced all four measured biodiversity indices: relative abundance, species richness, Pielou’s evenness and Shannon-Wiener Index, with water temperature also influencing relative abundance and altitude, species richness. Overall, the species composition showed that the sites were sampled later in the hydroperiod as evidenced by the dominance of active dispersers. The extended distribution of Prosthetops gladiator in the Eastern Cape highlights the need for extensive surveys to provide valuable insight into the distribution patterns of macroinvertebrates in the region. Furthermore, the interplay between physicochemical variables and biodiversity indices emphasized area, water temperature and pH as significant factors. However, their limited explanatory power suggests that obscure factors also influence the diversity cementing the need for further exploration of these enigmatic systems. Taxonomic challenges, especially regarding large branchiopods, hindered precise species distribution and conservation status determination. This study is the first to provide a comprehensive list of macroinvertebrates from temporary wetlands in the Maloti-Drakensberg Mountain. These results establish a valuable baseline for future biological monitoring studies, particularly in assessing species composition and responses to anthropogenic activities. It is a timeous study considering the sensitivity of these systems to climate change as they act as sentinel sites hence the need for foundational data on which to make future projections. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
Insights: elucidation of squalene monooxygenase inhibitors for lowering cholesterol in cardiovascular diseases
- Authors: Leoma, Mofeli Benedict
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434861 , vital:73111
- Description: Statins have been used to lower high cholesterol levels in the past few decades. However, several studies have shown that some people taking statins experience side effects over time, especially elderly patients, women of childbirth possibility, and children. Several studies have shown that the majority of people with underlying cardiovascular complications caused by high cholesterol are at a greater risk of fatality due to COVID-19, regardless of age and sex. The literature suggests that antimycotic squalene monooxygenase inhibitors, terbinafine and its derivatives, and anticholesterolemic squalene monooxygenase (SM) inhibitors could be another option and a safer remedy for lowering cholesterol in mammals. Molecular docking calculations, molecular dynamics (MD) simulations, molecular mechanics generalized born surface area (MM-GBSA) calculations, quantum mechanics/molecular mechanics calculations (QM/MM), and density functional theory (DFT) calculations were used in this study. An early stage in drug discovery, in which small molecular hits from high- throughput screening (HTS) are evaluated and undergo limited optimization to identify promising lead compounds, is referred to as lead generation. To address the first step of lead generation, the number of compounds to be tested was narrowed down, and the hit compounds that could be taken for further tests were obtained. Thus, the molecular docking technique was taken advantage of, which assisted us in identifying the antimycotic ligand SDZ 18, which had a good binding affinity of about -8,4 kcal mol−1. Another widely employed strategy, the molecular mechanics-generalized born surface area (MM-GBSA), was used to investigate the binding free energies of the protein-ligand complexes to validate the binding affinities obtained from molecular docking. Despite the excellent docking results, it must be emphasized that the stability of the ligand in the binding pocket must be investigated. To address this, the protein-ligand complexes were then taken through molecular dynamics for 100 ns simulations calculations which showed that the inhibitors stayed in the binding pocket with the RMSD values below 3.5 Å for most systems. This provided insight into a realistic model because the docked complexes were placed in conditions closer to the physiological environment at 300 K and 1.01325 bar, and in an explicitly solvated dynamic environment. Density functional theory (DFT) at the B3LPY level of theory using the standard 6-31G(d,p) basis set was used to assess the reactivity and other properties of the SM inhibitors. ONIOM calculations were performed to explain what was happening at the microscopic level by calculating the total energy of the complex. The aim of this project was to efficiently uncover the non-physical aspects of SM inhibitors with the help of computational techniques to identify new drugs that can lower high cholesterol levels. From a theoretical perspective, the results obtained from docking indicated that the antimycotic ligands SDZ SBA 586 18 and TNSA 84 (trisnor-squalene alcohol ) have good binding affinities, and the MM-GBSA method provided free energy calculations. MD results indicated that the stability of the ligand in the binding pocket was achieved during the 100 ns simulations. The HOMO-LUMO energy gaps obtained from DFT calculations provided information on the reactivity of the ligands. Other insights into the protein-ligand complexes were obtained from a hybrid ONIOM QM/MM study. , Thesis (MSc) -- Faculty of Science, Chemistry, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Leoma, Mofeli Benedict
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434861 , vital:73111
- Description: Statins have been used to lower high cholesterol levels in the past few decades. However, several studies have shown that some people taking statins experience side effects over time, especially elderly patients, women of childbirth possibility, and children. Several studies have shown that the majority of people with underlying cardiovascular complications caused by high cholesterol are at a greater risk of fatality due to COVID-19, regardless of age and sex. The literature suggests that antimycotic squalene monooxygenase inhibitors, terbinafine and its derivatives, and anticholesterolemic squalene monooxygenase (SM) inhibitors could be another option and a safer remedy for lowering cholesterol in mammals. Molecular docking calculations, molecular dynamics (MD) simulations, molecular mechanics generalized born surface area (MM-GBSA) calculations, quantum mechanics/molecular mechanics calculations (QM/MM), and density functional theory (DFT) calculations were used in this study. An early stage in drug discovery, in which small molecular hits from high- throughput screening (HTS) are evaluated and undergo limited optimization to identify promising lead compounds, is referred to as lead generation. To address the first step of lead generation, the number of compounds to be tested was narrowed down, and the hit compounds that could be taken for further tests were obtained. Thus, the molecular docking technique was taken advantage of, which assisted us in identifying the antimycotic ligand SDZ 18, which had a good binding affinity of about -8,4 kcal mol−1. Another widely employed strategy, the molecular mechanics-generalized born surface area (MM-GBSA), was used to investigate the binding free energies of the protein-ligand complexes to validate the binding affinities obtained from molecular docking. Despite the excellent docking results, it must be emphasized that the stability of the ligand in the binding pocket must be investigated. To address this, the protein-ligand complexes were then taken through molecular dynamics for 100 ns simulations calculations which showed that the inhibitors stayed in the binding pocket with the RMSD values below 3.5 Å for most systems. This provided insight into a realistic model because the docked complexes were placed in conditions closer to the physiological environment at 300 K and 1.01325 bar, and in an explicitly solvated dynamic environment. Density functional theory (DFT) at the B3LPY level of theory using the standard 6-31G(d,p) basis set was used to assess the reactivity and other properties of the SM inhibitors. ONIOM calculations were performed to explain what was happening at the microscopic level by calculating the total energy of the complex. The aim of this project was to efficiently uncover the non-physical aspects of SM inhibitors with the help of computational techniques to identify new drugs that can lower high cholesterol levels. From a theoretical perspective, the results obtained from docking indicated that the antimycotic ligands SDZ SBA 586 18 and TNSA 84 (trisnor-squalene alcohol ) have good binding affinities, and the MM-GBSA method provided free energy calculations. MD results indicated that the stability of the ligand in the binding pocket was achieved during the 100 ns simulations. The HOMO-LUMO energy gaps obtained from DFT calculations provided information on the reactivity of the ligands. Other insights into the protein-ligand complexes were obtained from a hybrid ONIOM QM/MM study. , Thesis (MSc) -- Faculty of Science, Chemistry, 2024
- Full Text:
- Date Issued: 2024-04-04
Investigating changes in pineapple (Aananas comosus) cultivation in the Eastern Cape, South Africa, from 1984 to 2020
- Authors: Marriner, Paul Joseph
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435354 , vital:73150
- Description: Land use and land cover change (LULCC) resulting from agricultural activities have significantly impacted landscape transformation and fragmentation. The Albany Thicket Biome in the Eastern Cape Province stands out for its exceptional vegetation diversity and remarkable rates of species endemism. However, the relationship between agricultural activities and the Albany Thicket Biome has not received sufficient attention in the literature, creating a significant gap in understanding the extent of landscape transformation and the vegetation's recovery rate. This study aims to address this gap by utilising remote sensing technologies to investigate the LULCC specifically caused by pineapple cultivation in the Lower Albany area between 1984 and 2020. Analysis, using remotely sensed imagery and spatial analytical tools, provide accurate identification of pineapple fields and enable monitoring of their effects on LULCC dynamics across a wide spatial and temporal scale. Complementary field assessments examined the impacts of pineapple cultivation on land use and cover. Twelve image classifiers were tested to identify the most appropriate technique for mapping pineapple fields, and the Supervised Pixel-based Support Vector Machine (SVM) image classifier was found to be the most suitable. Utilising Landsat 4, 5, 7, and 8 satellite imagery, 27 land cover maps were created, spanning the period from 1984 to 2020. Additionally, field verification was conducted at 59 randomly generated sites to validate the findings. Spatial analysis of the data revealed that the pineapple industry in the study area has expanded by 733 hectares since 1984. Significant land use changes were observed, including converting land to wildlife ranches, grazing areas, and alternative agricultural practices. The land cover analysis identified the emergence of pioneer species in former pineapple fields, suggesting the potential for Albany Thicket regrowth if appropriately managed. This research contributes to a better understanding of the impacts of pineapple cultivation on the Albany Thicket Biome and provides valuable insights for land use planning and monitoring efforts. A comprehensive assessment of LULCC dynamics can be achieved by utilising remote sensing techniques, informing sustainable land management practices in the study area and beyond. , Thesis (MSc) -- Faculty of Science, Geography, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Marriner, Paul Joseph
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435354 , vital:73150
- Description: Land use and land cover change (LULCC) resulting from agricultural activities have significantly impacted landscape transformation and fragmentation. The Albany Thicket Biome in the Eastern Cape Province stands out for its exceptional vegetation diversity and remarkable rates of species endemism. However, the relationship between agricultural activities and the Albany Thicket Biome has not received sufficient attention in the literature, creating a significant gap in understanding the extent of landscape transformation and the vegetation's recovery rate. This study aims to address this gap by utilising remote sensing technologies to investigate the LULCC specifically caused by pineapple cultivation in the Lower Albany area between 1984 and 2020. Analysis, using remotely sensed imagery and spatial analytical tools, provide accurate identification of pineapple fields and enable monitoring of their effects on LULCC dynamics across a wide spatial and temporal scale. Complementary field assessments examined the impacts of pineapple cultivation on land use and cover. Twelve image classifiers were tested to identify the most appropriate technique for mapping pineapple fields, and the Supervised Pixel-based Support Vector Machine (SVM) image classifier was found to be the most suitable. Utilising Landsat 4, 5, 7, and 8 satellite imagery, 27 land cover maps were created, spanning the period from 1984 to 2020. Additionally, field verification was conducted at 59 randomly generated sites to validate the findings. Spatial analysis of the data revealed that the pineapple industry in the study area has expanded by 733 hectares since 1984. Significant land use changes were observed, including converting land to wildlife ranches, grazing areas, and alternative agricultural practices. The land cover analysis identified the emergence of pioneer species in former pineapple fields, suggesting the potential for Albany Thicket regrowth if appropriately managed. This research contributes to a better understanding of the impacts of pineapple cultivation on the Albany Thicket Biome and provides valuable insights for land use planning and monitoring efforts. A comprehensive assessment of LULCC dynamics can be achieved by utilising remote sensing techniques, informing sustainable land management practices in the study area and beyond. , Thesis (MSc) -- Faculty of Science, Geography, 2024
- Full Text:
- Date Issued: 2024-04-04
Investigating playing-related musculoskeletal disorders in South African instrumental musicians
- Authors: Green, Erryn Mae
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435310 , vital:73146
- Description: This study, one of the first studies in SA to investigate PRMD in a wideranging sample of instrumental musicians using a validated self-report tool, shows that PRMDs are highly prevalent among a range of SA musicians and have a considerably negative effect on musicians’ performance ability and quality of life. However, making inferences about most affected genre or instrumental group was challenging due to the small sample sizes in some categories. The results from this study confirm that the nature of PRMD development is indeed multi-factorial, with factors numerous individual and playing-related factors playing a role in the development of PRMDs in SA musicians. Better support and education on health promotion for musicians, including awareness of and prevention strategies for PRMDs in the SA context are needed which may reduce the prevalence of PRMDs. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Green, Erryn Mae
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435310 , vital:73146
- Description: This study, one of the first studies in SA to investigate PRMD in a wideranging sample of instrumental musicians using a validated self-report tool, shows that PRMDs are highly prevalent among a range of SA musicians and have a considerably negative effect on musicians’ performance ability and quality of life. However, making inferences about most affected genre or instrumental group was challenging due to the small sample sizes in some categories. The results from this study confirm that the nature of PRMD development is indeed multi-factorial, with factors numerous individual and playing-related factors playing a role in the development of PRMDs in SA musicians. Better support and education on health promotion for musicians, including awareness of and prevention strategies for PRMDs in the SA context are needed which may reduce the prevalence of PRMDs. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2024
- Full Text:
- Date Issued: 2024-04-04
Investigating unimodal isolated signer-independent sign language recognition
- Authors: Marais, Marc Jason
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435343 , vital:73149
- Description: Sign language serves as the mode of communication for the Deaf and Hard of Hearing community, embodying a rich linguistic and cultural heritage. Recent Sign Language Recognition (SLR) system developments aim to facilitate seamless communication between the Deaf community and the broader society. However, most existing systems are limited by signer-dependent models, hindering their adaptability to diverse signing styles and signers, thus impeding their practical implementation in real-world scenarios. This research explores various unimodal approaches, both pose-based and vision-based, for isolated signer-independent SLR using RGB video input on the LSA64 and AUTSL datasets. The unimodal RGB-only input strategy provides a realistic SLR setting where alternative data sources are either unavailable or necessitate specialised equipment. Through systematic testing scenarios, isolated signer-independent SLR experiments are conducted on both datasets, primarily focusing on AUTSL – a signer-independent dataset. The vision-based R(2+1)D-18 model emerged as the top performer, achieving 90.64% accuracy on the unseen AUTSL dataset test split, closely followed by the pose-based Spatio- Temporal Graph Convolutional Network (ST-GCN) model with an accuracy of 89.95%. Furthermore, these models achieved comparable accuracies at a significantly lower computational demand. Notably, the pose-based approach demonstrates robust generalisation to substantial background and signer variation. Moreover, the pose-based approach demands significantly less computational power and training time than vision-based approaches. The proposed unimodal pose-based and vision-based systems were concluded to both be effective at classifying sign classes in the LSA64 and AUTSL datasets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Marais, Marc Jason
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435343 , vital:73149
- Description: Sign language serves as the mode of communication for the Deaf and Hard of Hearing community, embodying a rich linguistic and cultural heritage. Recent Sign Language Recognition (SLR) system developments aim to facilitate seamless communication between the Deaf community and the broader society. However, most existing systems are limited by signer-dependent models, hindering their adaptability to diverse signing styles and signers, thus impeding their practical implementation in real-world scenarios. This research explores various unimodal approaches, both pose-based and vision-based, for isolated signer-independent SLR using RGB video input on the LSA64 and AUTSL datasets. The unimodal RGB-only input strategy provides a realistic SLR setting where alternative data sources are either unavailable or necessitate specialised equipment. Through systematic testing scenarios, isolated signer-independent SLR experiments are conducted on both datasets, primarily focusing on AUTSL – a signer-independent dataset. The vision-based R(2+1)D-18 model emerged as the top performer, achieving 90.64% accuracy on the unseen AUTSL dataset test split, closely followed by the pose-based Spatio- Temporal Graph Convolutional Network (ST-GCN) model with an accuracy of 89.95%. Furthermore, these models achieved comparable accuracies at a significantly lower computational demand. Notably, the pose-based approach demonstrates robust generalisation to substantial background and signer variation. Moreover, the pose-based approach demands significantly less computational power and training time than vision-based approaches. The proposed unimodal pose-based and vision-based systems were concluded to both be effective at classifying sign classes in the LSA64 and AUTSL datasets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
Managing releases of Anagyrus vladimiri (Triapitsyn) to augment biocontrol of the citrus mealybug Planococcus citri (Risso) in South African citrus orchards
- Authors: Mommsen, Wayne Trevor
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434952 , vital:73118
- Description: In May 2019, South Korean inspectors rejected numerous grapefruit consignments from Letsitele, Hoedspruit and Onderberg in South Arica, because of live mealybug found on fruit. Growers expressed deep concern as mealybug management to a phytosanitary level was almost unattainable. Regular spray interventions for control of citrus black spot fungus, Phyllosticta citricarpa, and citrus thrips, Scirtothrips aurantii, cause repercussions in mealybug populations because they undermine the naturally occurring biocontrol complex. As part of an Integrated Pest Management (IPM) strategy, release of commercially produced parasitoids is common practice, to augment the naturally occurring beneficial insect populations. Prior knowledge of the harmful effects of insecticides on parasitoids is essential to IPM planning and the success of the biocontrol component in such a programme. Timing of augmentative releases to coincide with the phenology of citrus and the mealybug pest is also considered important for the successful establishment and control. Consequently, field trials were conducted to compare efficacy of early vs. late releases of Anagyrus vladimiri (Triapitsyn), an effective parasitoid of the citrus mealybug, Planococcus citri (Risso). Semi-field bioassays were conducted concurrently to determine the impact of various thripicides on A. vladimiri. The impact of sulfoxaflor, spinetoram, spirotetramat and prothiofos were rated harmless, as A. vladimiri mortality was lower than 25% after coming into contact with aged residues between 7 and 14 days old. October and November releases of A. vladimiri resulted in early parasitism and lowered peak-infestation of mealybug. January releases are possibly too late in grapefruit and lemon, open field, orchards, considering parasitism by A. vladimiri peaked in February. In mandarin orchards under net, percentage parasitism of 3rd instar mealybug increased a month later. Notably, at harvest, the difference in efficacy between treatments was not clear. This could be explained by high levels of natural parasitism observed in the treated and untreated orchards, which emphasises the importance of conservation biocontrol. In a second season, the proportion of hyperparasitoids captured (61%) from samples of mealybug-infested fruit was larger than the proportion of primary parasitoids, Anagyrus vladimiri, Coccidoxenoides perminutus (Girault) and Leptomastix dactylopii (Howard) (39%), which was far lower than the captures of eclosing primary parasitoids the previous season, which was 60%. The new discovery of Pseudaphycus sp. in citrus orchards in South Africa could be a key in explaining the uncontrollable levels of mealybug experienced and has drawn attention to a need for further understanding of ecological factors that influence biological control in citrus. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Mommsen, Wayne Trevor
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434952 , vital:73118
- Description: In May 2019, South Korean inspectors rejected numerous grapefruit consignments from Letsitele, Hoedspruit and Onderberg in South Arica, because of live mealybug found on fruit. Growers expressed deep concern as mealybug management to a phytosanitary level was almost unattainable. Regular spray interventions for control of citrus black spot fungus, Phyllosticta citricarpa, and citrus thrips, Scirtothrips aurantii, cause repercussions in mealybug populations because they undermine the naturally occurring biocontrol complex. As part of an Integrated Pest Management (IPM) strategy, release of commercially produced parasitoids is common practice, to augment the naturally occurring beneficial insect populations. Prior knowledge of the harmful effects of insecticides on parasitoids is essential to IPM planning and the success of the biocontrol component in such a programme. Timing of augmentative releases to coincide with the phenology of citrus and the mealybug pest is also considered important for the successful establishment and control. Consequently, field trials were conducted to compare efficacy of early vs. late releases of Anagyrus vladimiri (Triapitsyn), an effective parasitoid of the citrus mealybug, Planococcus citri (Risso). Semi-field bioassays were conducted concurrently to determine the impact of various thripicides on A. vladimiri. The impact of sulfoxaflor, spinetoram, spirotetramat and prothiofos were rated harmless, as A. vladimiri mortality was lower than 25% after coming into contact with aged residues between 7 and 14 days old. October and November releases of A. vladimiri resulted in early parasitism and lowered peak-infestation of mealybug. January releases are possibly too late in grapefruit and lemon, open field, orchards, considering parasitism by A. vladimiri peaked in February. In mandarin orchards under net, percentage parasitism of 3rd instar mealybug increased a month later. Notably, at harvest, the difference in efficacy between treatments was not clear. This could be explained by high levels of natural parasitism observed in the treated and untreated orchards, which emphasises the importance of conservation biocontrol. In a second season, the proportion of hyperparasitoids captured (61%) from samples of mealybug-infested fruit was larger than the proportion of primary parasitoids, Anagyrus vladimiri, Coccidoxenoides perminutus (Girault) and Leptomastix dactylopii (Howard) (39%), which was far lower than the captures of eclosing primary parasitoids the previous season, which was 60%. The new discovery of Pseudaphycus sp. in citrus orchards in South Africa could be a key in explaining the uncontrollable levels of mealybug experienced and has drawn attention to a need for further understanding of ecological factors that influence biological control in citrus. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
MeerKAT observations of the Abell 141 galaxy cluster
- Authors: Stanbury, Savannah Mae
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435378 , vital:73152
- Description: This study is aimed at being an overview and investigation of the behaviour and morphology of radio quiet (RQ) active galactic nuclei (AGN) in the radio and optical/near-infrared (NIR) bands. It is hoped that a concise description of the relation that exists between accretion activity and star formation (SF) will be achieved through utilization of multiwavelength astronomy analysis. This analysis includes the processing of data acquired from the MeerKAT International GHz Tiered Extragalactic Exploration survey (MIGHTEE), the Southern African Large Telescope (SALT), the Hyper Suprime Camera (HSC) mounted on the Subaru telescope and the VISTA telescope. In this thesis, several targets were observed using SALT spectroscopy, but a redshift of only one of them was obtained. Lastly, it was established that for the sample of RQ AGN studied, the AGN contribute a large fraction of the radio emission observed. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Stanbury, Savannah Mae
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435378 , vital:73152
- Description: This study is aimed at being an overview and investigation of the behaviour and morphology of radio quiet (RQ) active galactic nuclei (AGN) in the radio and optical/near-infrared (NIR) bands. It is hoped that a concise description of the relation that exists between accretion activity and star formation (SF) will be achieved through utilization of multiwavelength astronomy analysis. This analysis includes the processing of data acquired from the MeerKAT International GHz Tiered Extragalactic Exploration survey (MIGHTEE), the Southern African Large Telescope (SALT), the Hyper Suprime Camera (HSC) mounted on the Subaru telescope and the VISTA telescope. In this thesis, several targets were observed using SALT spectroscopy, but a redshift of only one of them was obtained. Lastly, it was established that for the sample of RQ AGN studied, the AGN contribute a large fraction of the radio emission observed. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-04-04
The effect of Gracilaria gracilis (Rhodophyta) on growth and behaviour of farmed abalone Haliotis midae when included in the abalone diet
- Madlala, Njabulo Praiseworth
- Authors: Madlala, Njabulo Praiseworth
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435332 , vital:73148
- Description: Abalone feed naturally on algae, but commercial abalone farms rely on formulated feed. The inclusion of algae with the formulated feed in the diet improves growth, health and feed conversion ratio (FCR). However, the kelp that is currently included in the feed is wild harvested and this resource is limited and under increasing pressure. Thus, there is a need to develop an alternative sustainable source of algae. The aim was to use Gracilaria gracilis algae (that was produced with mussel in an integrated multitrophic aquaculture system; IMTA) which was then included into abalone feeds and its influence on abalone growth, FCR and behaviour was evaluated. Sea-based G. gracilis was harvested off an existing IMTA system, manufactured into Abfeed® and fed to abalone to determine growth efficiency. Abalone (30 - 40 g abalone-1) originated from the same cohort at Whale Rock Abalone Farm (Pty) Ltd., Hermanus. Abalone were fed either: Abfeed® S34 only with no algae (G0, the control); Abfeed® with sea-based IMTA G. gracilis included in the formulated feed at 0.75 % (G0.75); 1.50 % (G1.50); 3.00 % (G3.00); 6.00 % (G6.00); and 12.00 % (G12.00) inclusion; and Abfeed® S34 with fresh-live G. gracilis (cultured at Whale Rock Abalone Farm (Pty) Ltd.; Gfresh + S34s). Abalone shell length, whole body mass and FCR were measured at a four-months interval over eight months (two growth cycles). Differences in behaviour (i.e., level of activity and feeding activity) were also monitored and comparing between treatments. There was significant difference in mean whole-body mass between treatments (RMANOVA, F(6, 29) = 3.71, p = 0.007). Abalone fed the diet with the highest inclusion of dry G. gracilis (G12.00) had a lower mean whole-body mass value (74.13 ± 2.94 g abalone-1) than abalone from all the other diets after eight months. There was a negative relationship between the condition factor and dry G. gracilis inclusion rate for the first growth cycle (y = - 0.0044x + 1.222, R2 = 0.24, p=0.009) and second-growth cycle (y = - 0.0096x + 1.2233, R2 = 0.51, p = 0.00002). Abalone receiving fresh G. gracilis were less quiescent and more alert on the first hours after food distribution (p < 0.05). The same proportion of abalone with G0, G0.75, and G6.00 G. gracilis inclusion were feeding all along the 9 hours post-feeding. This study will potentially reduce the dependence of abalone farms on wild-harvested kelp in formulated feed. This will improve their contribution to a more environmentally sustainable global production of sea food products, making them more competitive on international markets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Madlala, Njabulo Praiseworth
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435332 , vital:73148
- Description: Abalone feed naturally on algae, but commercial abalone farms rely on formulated feed. The inclusion of algae with the formulated feed in the diet improves growth, health and feed conversion ratio (FCR). However, the kelp that is currently included in the feed is wild harvested and this resource is limited and under increasing pressure. Thus, there is a need to develop an alternative sustainable source of algae. The aim was to use Gracilaria gracilis algae (that was produced with mussel in an integrated multitrophic aquaculture system; IMTA) which was then included into abalone feeds and its influence on abalone growth, FCR and behaviour was evaluated. Sea-based G. gracilis was harvested off an existing IMTA system, manufactured into Abfeed® and fed to abalone to determine growth efficiency. Abalone (30 - 40 g abalone-1) originated from the same cohort at Whale Rock Abalone Farm (Pty) Ltd., Hermanus. Abalone were fed either: Abfeed® S34 only with no algae (G0, the control); Abfeed® with sea-based IMTA G. gracilis included in the formulated feed at 0.75 % (G0.75); 1.50 % (G1.50); 3.00 % (G3.00); 6.00 % (G6.00); and 12.00 % (G12.00) inclusion; and Abfeed® S34 with fresh-live G. gracilis (cultured at Whale Rock Abalone Farm (Pty) Ltd.; Gfresh + S34s). Abalone shell length, whole body mass and FCR were measured at a four-months interval over eight months (two growth cycles). Differences in behaviour (i.e., level of activity and feeding activity) were also monitored and comparing between treatments. There was significant difference in mean whole-body mass between treatments (RMANOVA, F(6, 29) = 3.71, p = 0.007). Abalone fed the diet with the highest inclusion of dry G. gracilis (G12.00) had a lower mean whole-body mass value (74.13 ± 2.94 g abalone-1) than abalone from all the other diets after eight months. There was a negative relationship between the condition factor and dry G. gracilis inclusion rate for the first growth cycle (y = - 0.0044x + 1.222, R2 = 0.24, p=0.009) and second-growth cycle (y = - 0.0096x + 1.2233, R2 = 0.51, p = 0.00002). Abalone receiving fresh G. gracilis were less quiescent and more alert on the first hours after food distribution (p < 0.05). The same proportion of abalone with G0, G0.75, and G6.00 G. gracilis inclusion were feeding all along the 9 hours post-feeding. This study will potentially reduce the dependence of abalone farms on wild-harvested kelp in formulated feed. This will improve their contribution to a more environmentally sustainable global production of sea food products, making them more competitive on international markets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
The use of gabions as a tool for ecological engineering
- Authors: Seath, Jessica Lauren
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434987 , vital:73121
- Description: Anthropogenic activities are centred in coastal ecosystems, including the development of harbours and/or marinas. The artificial structures used in coastal development typically has a different composition, orientation and level of complexity to that of natural ecosystems contributing to loss of biodiversity and increased incidence of invasive species. Ecological engineering research is attempting to identify different types of structures and materials that can increase species diversity and target species of conservation concern in coastal systems. The aim of this study was to investigate the efficacy of gabions (rock filled structures) as an ecological engineering tool by comparing community structure on these structures with pre-existing seawall structures within a small harbour and marina in South Africa (Knysna Harbour). The objectives of the study were to compare the differences in; 1) fish and; 2) colonising organisms’ diversity and composition between two artificial structures. Thirteen gabion boxes were deployed in Knysna Harbour and together with corresponding seawalls, monitored quarterly over a period of 12 months (August 2020 – August 2021) to assess taxon and functional richness, diversity, abundance and composition of fish, invertebrate and algal species. Physico-chemical characteristics of the water body were also monitored quarterly. Remote underwater video systems were used to quantify MaxN (maximum number of a fish species in the frame at any one time during each set that gives an indication of relative abundance) and identify fish species. The results of the two-way crossed ANOVAs indicated that gabion habitats recruited greater numbers and more types of fish species and from more functional groups than the seawalls, especially omnivorous and carnivorous fish. Additionally, photoquadrats were used to quantify percentage cover, counts and to identify colonising taxa. The results of the two-way crossed ANOVAs indicated that gabions hosted greater numbers of species resulting in a higher overall diversity and abundance of colonising organisms than seawalls. By contrast, the seawalls supported more types of functional groups of colonising organisms than gabions, largely due to abundances of different algal species. The results from the crossed PERMANOVAs indicated that the composition of fish and colonising organisms were vastly different from one another, and that each habitat was supporting very different functional groups. Results indicate that whilst both gabions and seawalls contain several alien species, that the ratio of native to alien species is higher in gabion habitats. Additionally, this research observed that gabion structures hosted four species listed on the IUCN Red List of Threatened Species. This study has highlighted that the use of gabions (with their natural increased complexity) could be important to consider for the future of urban coastal development in harbours such as in Knysna Harbour. Ecological engineering projects using gabions have the potential to be used in South Africa in projects that aim at increasing biodiversity in urban coastal environments. As well as increasing the settlement and abundance of habitat-forming ecosystem engineers to ensure the long-term stability of these ecosystems. They can be used both in the development of new costal development projects as well as in an ad-hoc fashion where they can be interspersed on seawalls in harbours. Additionally, gabions have the potential to be used in projects that target species of conservation concern such as the endangered Knysna Seahorse (Hippocampus capensis). It is, however, important to monitor the invasion by non-native species in future ecological engineering projects in South Africa as well as their potential for creating ecological traps (a situation in which an organism may be convinced to settle in a low-quality habitat) for certain species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Seath, Jessica Lauren
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434987 , vital:73121
- Description: Anthropogenic activities are centred in coastal ecosystems, including the development of harbours and/or marinas. The artificial structures used in coastal development typically has a different composition, orientation and level of complexity to that of natural ecosystems contributing to loss of biodiversity and increased incidence of invasive species. Ecological engineering research is attempting to identify different types of structures and materials that can increase species diversity and target species of conservation concern in coastal systems. The aim of this study was to investigate the efficacy of gabions (rock filled structures) as an ecological engineering tool by comparing community structure on these structures with pre-existing seawall structures within a small harbour and marina in South Africa (Knysna Harbour). The objectives of the study were to compare the differences in; 1) fish and; 2) colonising organisms’ diversity and composition between two artificial structures. Thirteen gabion boxes were deployed in Knysna Harbour and together with corresponding seawalls, monitored quarterly over a period of 12 months (August 2020 – August 2021) to assess taxon and functional richness, diversity, abundance and composition of fish, invertebrate and algal species. Physico-chemical characteristics of the water body were also monitored quarterly. Remote underwater video systems were used to quantify MaxN (maximum number of a fish species in the frame at any one time during each set that gives an indication of relative abundance) and identify fish species. The results of the two-way crossed ANOVAs indicated that gabion habitats recruited greater numbers and more types of fish species and from more functional groups than the seawalls, especially omnivorous and carnivorous fish. Additionally, photoquadrats were used to quantify percentage cover, counts and to identify colonising taxa. The results of the two-way crossed ANOVAs indicated that gabions hosted greater numbers of species resulting in a higher overall diversity and abundance of colonising organisms than seawalls. By contrast, the seawalls supported more types of functional groups of colonising organisms than gabions, largely due to abundances of different algal species. The results from the crossed PERMANOVAs indicated that the composition of fish and colonising organisms were vastly different from one another, and that each habitat was supporting very different functional groups. Results indicate that whilst both gabions and seawalls contain several alien species, that the ratio of native to alien species is higher in gabion habitats. Additionally, this research observed that gabion structures hosted four species listed on the IUCN Red List of Threatened Species. This study has highlighted that the use of gabions (with their natural increased complexity) could be important to consider for the future of urban coastal development in harbours such as in Knysna Harbour. Ecological engineering projects using gabions have the potential to be used in South Africa in projects that aim at increasing biodiversity in urban coastal environments. As well as increasing the settlement and abundance of habitat-forming ecosystem engineers to ensure the long-term stability of these ecosystems. They can be used both in the development of new costal development projects as well as in an ad-hoc fashion where they can be interspersed on seawalls in harbours. Additionally, gabions have the potential to be used in projects that target species of conservation concern such as the endangered Knysna Seahorse (Hippocampus capensis). It is, however, important to monitor the invasion by non-native species in future ecological engineering projects in South Africa as well as their potential for creating ecological traps (a situation in which an organism may be convinced to settle in a low-quality habitat) for certain species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
The “Inadequately Married”: extending the putative marriage doctrine to assist vulnerable parties in invalid customary marriages
- Authors: Mavindidze, Tafadzwa Naomi
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434839 , vital:73109
- Description: This thesis considers whether the putative marriage doctrine can be developed to assist parties that find themselves in invalid customary marriages. The thesis focuses on situations where a customary marriage is considered invalid in circumstances where a party does not meet specific legislative or customary requirements. In most instances, these women are left without protection and regulation of the proprietary consequences of the so-called marital estate. The research considers the putative marriage doctrine as a remedy to this challenge. The doctrine is utilised where one or both parties believe in good faith that their marriage is valid when, in fact, one or more of the material requirements for marriage have not been met. Currently, the judgment of Zulu v Zulu 2008 (4) SA 12 (D) impedes the doctrine’s application in polygamous customary marriages and thus impedes its use by women who are found in invalid customary marriages. The research carries out a comparative analysis of Californian and Namibian family law, in order to consider how other jurisdictions deal with similar situations. The thesis further proposes a framework to develop the putative marriage doctrine in a way that ensures the protection of customary wives in subsequent marriages that are declared invalid. , Thesis (LLM) -- Faculty of Law, Law, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Mavindidze, Tafadzwa Naomi
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434839 , vital:73109
- Description: This thesis considers whether the putative marriage doctrine can be developed to assist parties that find themselves in invalid customary marriages. The thesis focuses on situations where a customary marriage is considered invalid in circumstances where a party does not meet specific legislative or customary requirements. In most instances, these women are left without protection and regulation of the proprietary consequences of the so-called marital estate. The research considers the putative marriage doctrine as a remedy to this challenge. The doctrine is utilised where one or both parties believe in good faith that their marriage is valid when, in fact, one or more of the material requirements for marriage have not been met. Currently, the judgment of Zulu v Zulu 2008 (4) SA 12 (D) impedes the doctrine’s application in polygamous customary marriages and thus impedes its use by women who are found in invalid customary marriages. The research carries out a comparative analysis of Californian and Namibian family law, in order to consider how other jurisdictions deal with similar situations. The thesis further proposes a framework to develop the putative marriage doctrine in a way that ensures the protection of customary wives in subsequent marriages that are declared invalid. , Thesis (LLM) -- Faculty of Law, Law, 2024
- Full Text:
- Date Issued: 2024-04-04