Network analysis of trophic linkages in two sub-tropical estuaries along the South-East coast of South Africa
- Authors: Vosloo, Mathys Christiaan
- Date: 2012
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10708 , http://hdl.handle.net/10948/d1010966 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Description: Estuaries are some of the most productive yet threatened ecosystems in the world. Despite their importance they face significant threats through changes to river flow, eutrophication, rapid population growth long the caost and harvesting of natural resources. A number of international studies have been conducted investigating the structure and functioning of an array of ecosystems using ecological network analysis. Energy flow networks have been contsructed for coastal, lagoonal, intertidial and, most notably, permantently open estuaries. Despite the valualble insights contributed by these and other studies, a lack of information on the majority of estuarine ecosystems exists.
- Full Text:
- Date Issued: 2012
- Authors: Vosloo, Mathys Christiaan
- Date: 2012
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10708 , http://hdl.handle.net/10948/d1010966 , Estuaries -- South Africa -- Eastern Cape , Estuarine ecology , Network analysis (Planning)
- Description: Estuaries are some of the most productive yet threatened ecosystems in the world. Despite their importance they face significant threats through changes to river flow, eutrophication, rapid population growth long the caost and harvesting of natural resources. A number of international studies have been conducted investigating the structure and functioning of an array of ecosystems using ecological network analysis. Energy flow networks have been contsructed for coastal, lagoonal, intertidial and, most notably, permantently open estuaries. Despite the valualble insights contributed by these and other studies, a lack of information on the majority of estuarine ecosystems exists.
- Full Text:
- Date Issued: 2012
Network management for community networks
- Authors: Wells, Daniel David
- Date: 2010 , 2010-03-26
- Subjects: Computer networks -- Management , Internet -- South Africa , Internet -- Management , Broadband communication systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4643 , http://hdl.handle.net/10962/d1006587
- Description: Community networks (in South Africa and Africa) are often serviced by limited bandwidth network backhauls. Relative to the basic needs of the community, this is an expensive ongoing concern. In many cases the Internet connection is shared among multiple sites. Community networks may also have a lack of technical personnel to maintain a network of this nature. Hence, there is a demand for a system which will monitor and manage bandwidth use, as well as network use. The proposed solution for community networks and the focus within this dissertation, is a system of two parts. A Community Access Point (CAP) is located at each site within the community network. This provides the hosts and servers at that site with access to services on the community network and the Internet, it is the site's router. The CAP provides a web based interface (CAPgui) which allows configuration of the device and viewing of simple monitoring statistics. The Access Concentrator (AC) is the default router for the CAPs and the gateway to the Internet. It provides authenticated and encrypted communication between the network sites. The AC performs several monitoring functions, both for the individual sites and for the upstream Internet connection. The AC provides a means for centrally managing and effectively allocating Internet bandwidth by using the web based interface (ACgui). Bandwidth use can be allocated per user, per host and per site. The system is maintainable, extendable and customisable for different network architectures. The system was deployed successfully to two community networks. The Centre of Excellence (CoE) testbed network is a peri-urban network deployment whereas the Siyakhula Living Lab (SLL) network is a rural deployment. The results gathered conclude that the project was successful as the deployed system is more robust and more manageable than the previous systems.
- Full Text:
- Date Issued: 2010
- Authors: Wells, Daniel David
- Date: 2010 , 2010-03-26
- Subjects: Computer networks -- Management , Internet -- South Africa , Internet -- Management , Broadband communication systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4643 , http://hdl.handle.net/10962/d1006587
- Description: Community networks (in South Africa and Africa) are often serviced by limited bandwidth network backhauls. Relative to the basic needs of the community, this is an expensive ongoing concern. In many cases the Internet connection is shared among multiple sites. Community networks may also have a lack of technical personnel to maintain a network of this nature. Hence, there is a demand for a system which will monitor and manage bandwidth use, as well as network use. The proposed solution for community networks and the focus within this dissertation, is a system of two parts. A Community Access Point (CAP) is located at each site within the community network. This provides the hosts and servers at that site with access to services on the community network and the Internet, it is the site's router. The CAP provides a web based interface (CAPgui) which allows configuration of the device and viewing of simple monitoring statistics. The Access Concentrator (AC) is the default router for the CAPs and the gateway to the Internet. It provides authenticated and encrypted communication between the network sites. The AC performs several monitoring functions, both for the individual sites and for the upstream Internet connection. The AC provides a means for centrally managing and effectively allocating Internet bandwidth by using the web based interface (ACgui). Bandwidth use can be allocated per user, per host and per site. The system is maintainable, extendable and customisable for different network architectures. The system was deployed successfully to two community networks. The Centre of Excellence (CoE) testbed network is a peri-urban network deployment whereas the Siyakhula Living Lab (SLL) network is a rural deployment. The results gathered conclude that the project was successful as the deployed system is more robust and more manageable than the previous systems.
- Full Text:
- Date Issued: 2010
Network simulation for professional audio networks
- Authors: Otten, Fred
- Date: 2015
- Subjects: Sound engineers , Ethernet (Local area network system) , Computer networks , Computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4713 , http://hdl.handle.net/10962/d1017935
- Description: Audio Engineers are required to design and deploy large multi-channel sound systems which meet a set of requirements and use networking technologies such as Firewire and Ethernet AVB. Bandwidth utilisation and parameter groupings are among the factors which need to be considered in these designs. An implementation of an extensible, generic simulation framework would allow audio engineers to easily compare protocols and networking technologies and get near real time responses with regards to bandwidth utilisation. Our hypothesis is that an application-level capability can be developed which uses a network simulation framework to enable this process and enhances the audio engineer’s experience of designing and configuring a network. This thesis presents a new, extensible simulation framework which can be utilised to simulate professional audio networks. This framework is utilised to develop an application - AudioNetSim - based on the requirements of an audio engineer. The thesis describes the AudioNetSim models and implementations for Ethernet AVB, Firewire and the AES- 64 control protocol. AudioNetSim enables bandwidth usage determination for any network configuration and connection scenario and is used to compare Firewire and Ethernet AVB bandwidth utilisation. It also applies graph theory to the circular join problem and provides a solution to detect circular joins.
- Full Text:
- Date Issued: 2015
- Authors: Otten, Fred
- Date: 2015
- Subjects: Sound engineers , Ethernet (Local area network system) , Computer networks , Computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4713 , http://hdl.handle.net/10962/d1017935
- Description: Audio Engineers are required to design and deploy large multi-channel sound systems which meet a set of requirements and use networking technologies such as Firewire and Ethernet AVB. Bandwidth utilisation and parameter groupings are among the factors which need to be considered in these designs. An implementation of an extensible, generic simulation framework would allow audio engineers to easily compare protocols and networking technologies and get near real time responses with regards to bandwidth utilisation. Our hypothesis is that an application-level capability can be developed which uses a network simulation framework to enable this process and enhances the audio engineer’s experience of designing and configuring a network. This thesis presents a new, extensible simulation framework which can be utilised to simulate professional audio networks. This framework is utilised to develop an application - AudioNetSim - based on the requirements of an audio engineer. The thesis describes the AudioNetSim models and implementations for Ethernet AVB, Firewire and the AES- 64 control protocol. AudioNetSim enables bandwidth usage determination for any network configuration and connection scenario and is used to compare Firewire and Ethernet AVB bandwidth utilisation. It also applies graph theory to the circular join problem and provides a solution to detect circular joins.
- Full Text:
- Date Issued: 2015
Network-layer reservation TDM for ad-hoc 802.11 networks
- Authors: Duff, Kevin Craig
- Date: 2008
- Subjects: Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4574 , http://hdl.handle.net/10962/d1002773 , Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Description: Ad-Hoc mesh networks offer great promise. Low-cost ad-hoc mesh networks can be built using popular IEEE 802.11 equipment, but such networks are unable to guarantee each node a fair share of bandwidth. Furthermore, hidden node problems cause collisions which can cripple the throughput of a network. This research proposes a novel mechanism which is able to overcome hidden node problems and provide fair bandwidth sharing among nodes on ad-hoc 802.11 networks, and can be implemented on existing network devices. The scheme uses TDM (time division multiplexing) with slot reservation. A distributed beacon packet latency measurement mechanism is used to achieve node synchronisation. The distributed nature of the mechanism makes it applicable to ad-hoc 802.11 networks, which can either grow or fragment dynamically.
- Full Text:
- Date Issued: 2008
- Authors: Duff, Kevin Craig
- Date: 2008
- Subjects: Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4574 , http://hdl.handle.net/10962/d1002773 , Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Description: Ad-Hoc mesh networks offer great promise. Low-cost ad-hoc mesh networks can be built using popular IEEE 802.11 equipment, but such networks are unable to guarantee each node a fair share of bandwidth. Furthermore, hidden node problems cause collisions which can cripple the throughput of a network. This research proposes a novel mechanism which is able to overcome hidden node problems and provide fair bandwidth sharing among nodes on ad-hoc 802.11 networks, and can be implemented on existing network devices. The scheme uses TDM (time division multiplexing) with slot reservation. A distributed beacon packet latency measurement mechanism is used to achieve node synchronisation. The distributed nature of the mechanism makes it applicable to ad-hoc 802.11 networks, which can either grow or fragment dynamically.
- Full Text:
- Date Issued: 2008
Networking English Teachers' Conference
- Authors: Badat, Saleem
- Date: 2014-12-04
- Language: English
- Type: Text
- Identifier: vital:7785 , http://hdl.handle.net/10962/d1015940
- Full Text:
- Authors: Badat, Saleem
- Date: 2014-12-04
- Language: English
- Type: Text
- Identifier: vital:7785 , http://hdl.handle.net/10962/d1015940
- Full Text:
Networking: enabling professional development and institutionalisation of environmental education courses in Southern Africa
- Authors: Lupele, Justin Kalaba
- Date: 2007
- Subjects: SADC Regional Environmental Education Programme Education -- Africa, Southern Environmental education -- Africa, Southern Environmental education -- Study and teaching -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1678 , http://hdl.handle.net/10962/d1003561
- Description: This study was aimed at understanding how networking can enable or constrain professional development and institutionalisation of environmental education courses in southern Africa in the context of the Course Development Network (CDN), a project of the Southern Africa Development Community Regional Environmental Education Programme (SADC REEP). The study involved 12 institutions (each institution was represented in the CDN by a staff member) in eight SADC member states. It was contextualized through a review of social, political, environmental and educational developments in southern Africa, with specific reference to regionalization processes, as the SADC REEF is constituted under this post-colonial political framework. Relational philosophy informed the research process and methodology. The philosophy underpinned three distinct, yet related theoretical perspectives namely: critical realism, which provided the ontological perspectives of the study; Actor Network Theory; and Community of Practice, which provided the epistemological perspectives. Data was generated during a 33-month period in southern Africa and 12 months in the United Kingdom (and on a one week visit to Italy). During a 12 month Split Site Commonwealth Scholarship award, tenable at Manchester Metropolitan University in the United Kingdom, I studied the Environment and School Initiative (ENSI) and the Sustainability Education for European Primary Schools (SEEPS), to examine whether the same mechanisms that made them successful would apply to the CDN in the context of southern Africa. While these two case studies provided useful insight into the relationship between networking, professional development and institutionalization of environmental education programmes, they were not the main focus of the research. Data analysis was mainly through inductive, abductive and retroductive modes of inference. Inductive data analysis was done by means of Nvivo – a computer software package used for qualitative data analysis. The software aided in revealing features and relationships in the data in more depth as it allowed flexibility in working with data. Abduction is the interpretation of a phenomenon by means of a conceptual framework. In this study, I used Actor Network Theory (ANT) (Latour & Woolgar, 1979; Callon, 1986) and Community of Practice (COP) (Lave, 1988; Lave & Wenger, 1991; Fullan, 2003) as analytical conceptual frameworks to probe networking and professional development respectively. Data on institutionalisation of environmental education courses was analysed by means of retroductive mode of inference, which is a thought operation that enables the understanding of social reality beyond what is empirically observable or experienced. This study reveals that there were a number of necessary (internal) and contingent (external) factors that enabled or constrained networking, professional development and institutionalisation of environmental education courses in the context of the CDN. The key factors included existing cultural capital, donor political economy, power relations, poverty related factors and social transformation trends. The study found that relational approaches and the use of three relational theoretical lenses provided a broader lens which enabled this study to identify different dynamics, greater ontological depth and understanding of the relational dynamics and relations at play in the CDN beyond the participants' experience and observable events. The study also contends that networking can provide a support structure for social transformation and change in environmental education.
- Full Text:
- Date Issued: 2007
- Authors: Lupele, Justin Kalaba
- Date: 2007
- Subjects: SADC Regional Environmental Education Programme Education -- Africa, Southern Environmental education -- Africa, Southern Environmental education -- Study and teaching -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1678 , http://hdl.handle.net/10962/d1003561
- Description: This study was aimed at understanding how networking can enable or constrain professional development and institutionalisation of environmental education courses in southern Africa in the context of the Course Development Network (CDN), a project of the Southern Africa Development Community Regional Environmental Education Programme (SADC REEP). The study involved 12 institutions (each institution was represented in the CDN by a staff member) in eight SADC member states. It was contextualized through a review of social, political, environmental and educational developments in southern Africa, with specific reference to regionalization processes, as the SADC REEF is constituted under this post-colonial political framework. Relational philosophy informed the research process and methodology. The philosophy underpinned three distinct, yet related theoretical perspectives namely: critical realism, which provided the ontological perspectives of the study; Actor Network Theory; and Community of Practice, which provided the epistemological perspectives. Data was generated during a 33-month period in southern Africa and 12 months in the United Kingdom (and on a one week visit to Italy). During a 12 month Split Site Commonwealth Scholarship award, tenable at Manchester Metropolitan University in the United Kingdom, I studied the Environment and School Initiative (ENSI) and the Sustainability Education for European Primary Schools (SEEPS), to examine whether the same mechanisms that made them successful would apply to the CDN in the context of southern Africa. While these two case studies provided useful insight into the relationship between networking, professional development and institutionalization of environmental education programmes, they were not the main focus of the research. Data analysis was mainly through inductive, abductive and retroductive modes of inference. Inductive data analysis was done by means of Nvivo – a computer software package used for qualitative data analysis. The software aided in revealing features and relationships in the data in more depth as it allowed flexibility in working with data. Abduction is the interpretation of a phenomenon by means of a conceptual framework. In this study, I used Actor Network Theory (ANT) (Latour & Woolgar, 1979; Callon, 1986) and Community of Practice (COP) (Lave, 1988; Lave & Wenger, 1991; Fullan, 2003) as analytical conceptual frameworks to probe networking and professional development respectively. Data on institutionalisation of environmental education courses was analysed by means of retroductive mode of inference, which is a thought operation that enables the understanding of social reality beyond what is empirically observable or experienced. This study reveals that there were a number of necessary (internal) and contingent (external) factors that enabled or constrained networking, professional development and institutionalisation of environmental education courses in the context of the CDN. The key factors included existing cultural capital, donor political economy, power relations, poverty related factors and social transformation trends. The study found that relational approaches and the use of three relational theoretical lenses provided a broader lens which enabled this study to identify different dynamics, greater ontological depth and understanding of the relational dynamics and relations at play in the CDN beyond the participants' experience and observable events. The study also contends that networking can provide a support structure for social transformation and change in environmental education.
- Full Text:
- Date Issued: 2007
Neura
- Chabarwa Musunda Moyo, performer not specified, composer not specified, Tracey, Hugh
- Authors: Chabarwa Musunda Moyo , performer not specified , composer not specified , Tracey, Hugh
- Date: 1948
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe city not specified f-rh
- Language: nyanja
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/367237 , vital:65953 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , ACO199-AQ11
- Description: Indigenous music
- Full Text: false
- Date Issued: 1948
- Authors: Chabarwa Musunda Moyo , performer not specified , composer not specified , Tracey, Hugh
- Date: 1948
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe city not specified f-rh
- Language: nyanja
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/367237 , vital:65953 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , ACO199-AQ11
- Description: Indigenous music
- Full Text: false
- Date Issued: 1948
Neura
- Muduma-Zakawa-Chigogo, Performer not specified, Composer not specified, Tracey, Hugh
- Authors: Muduma-Zakawa-Chigogo , Performer not specified , Composer not specified , Tracey, Hugh
- Date: 1933
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe City not specified f-rh
- Language: Shona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/342493 , vital:62898 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , TP3749-000
- Description: Indigenous music
- Full Text: false
- Date Issued: 1933
- Authors: Muduma-Zakawa-Chigogo , Performer not specified , Composer not specified , Tracey, Hugh
- Date: 1933
- Subjects: Folk Music , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe City not specified f-rh
- Language: Shona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/342493 , vital:62898 , International Library of African Music, Rhodes University, Makhanda, South Africa , Hugh Tracey Commercial Records, Rhodes University, Makhanda, South Africa , TP3749-000
- Description: Indigenous music
- Full Text: false
- Date Issued: 1933
Neural network fault diagnosis system for a diesel-electric locomotive's closed loop excitation control system
- Authors: Barnard, Morne
- Date: 2017
- Subjects: Neural networks (Computer science) Diesel locomotives -- Motors -- Control systems
- Language: English
- Type: Thesis , Masters , MEng
- Identifier: http://hdl.handle.net/10948/15955 , vital:28294
- Description: In closed loop control systems fault isolation is extremely difficult due to the fact that if feedbacks are corrupted or actuators can’t produce a desired output, a system reacts due to an increase in error between the measured variable and the set input variable, which can cause oscillations. The goal of this project is to develop a fault detection and isolation system for the isolation of faults, which cause oscillatory conditions on a GE Diesel-Electric Locomotive’s excitation control system. The proposed system will illustrate the use of artificial neural networks as a replacement to classical analytical models. The artificial neural network model’s design will be based on model-based dedicated observer theory to isolate sensor, as well as component faults, where observer theory will be utilised to effectively select input-output data configurations for detection of sensor and component faults causing oscillations. Owing to the nature of the locomotive’s data acquisition abilities, the model-based observer design will utilise historical data to design an effective model of the system which will be used to perform offline sampled fault detection. This method is proposed as an alternative to trend checking, data mining, etc. Faults are thus detected through the use of an offline model-based dedicated observer residual generator. With the use of a neural network a number of parameters affect the accuracy of the network where the primary source of ensuring an accurate model is training. The project highlights and experiments with these parameters to ensure an accurate model is trained with the use of the gradient descent training algorithm. The parameters which are considered are learning rate, hidden layer neurons, momentum and data preparation. The project will also provide a literature review on residual evaluation techniques used in practice and describe and evaluate the proposed method to perform residual evaluation for this specific application. The proposed method for residual evaluation was based on two principles, namely the moving average, as well as the simple thresholding techniques. The developed FDI system’s performance was measured against known faults and produced 100% accuracy for the detection and isolation of sensor and components causing oscillatory conditions on the locomotive’s excitation system.
- Full Text:
- Date Issued: 2017
- Authors: Barnard, Morne
- Date: 2017
- Subjects: Neural networks (Computer science) Diesel locomotives -- Motors -- Control systems
- Language: English
- Type: Thesis , Masters , MEng
- Identifier: http://hdl.handle.net/10948/15955 , vital:28294
- Description: In closed loop control systems fault isolation is extremely difficult due to the fact that if feedbacks are corrupted or actuators can’t produce a desired output, a system reacts due to an increase in error between the measured variable and the set input variable, which can cause oscillations. The goal of this project is to develop a fault detection and isolation system for the isolation of faults, which cause oscillatory conditions on a GE Diesel-Electric Locomotive’s excitation control system. The proposed system will illustrate the use of artificial neural networks as a replacement to classical analytical models. The artificial neural network model’s design will be based on model-based dedicated observer theory to isolate sensor, as well as component faults, where observer theory will be utilised to effectively select input-output data configurations for detection of sensor and component faults causing oscillations. Owing to the nature of the locomotive’s data acquisition abilities, the model-based observer design will utilise historical data to design an effective model of the system which will be used to perform offline sampled fault detection. This method is proposed as an alternative to trend checking, data mining, etc. Faults are thus detected through the use of an offline model-based dedicated observer residual generator. With the use of a neural network a number of parameters affect the accuracy of the network where the primary source of ensuring an accurate model is training. The project highlights and experiments with these parameters to ensure an accurate model is trained with the use of the gradient descent training algorithm. The parameters which are considered are learning rate, hidden layer neurons, momentum and data preparation. The project will also provide a literature review on residual evaluation techniques used in practice and describe and evaluate the proposed method to perform residual evaluation for this specific application. The proposed method for residual evaluation was based on two principles, namely the moving average, as well as the simple thresholding techniques. The developed FDI system’s performance was measured against known faults and produced 100% accuracy for the detection and isolation of sensor and components causing oscillatory conditions on the locomotive’s excitation system.
- Full Text:
- Date Issued: 2017
Neural network-based ionospheric modelling over the South African region
- McKinnell, Lee-Anne, Poole, Allon W V
- Authors: McKinnell, Lee-Anne , Poole, Allon W V
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6795 , http://hdl.handle.net/10962/d1003839
- Description: During the past decade, South African scientists have pioneered research in the field of ionospheric modelling using the technique of neural networks (NNs). Global ionospheric models have always been insufficient for the South African region owing to an historical paucity of available data. Within the past 10 years, however, three new ionospheric sounders have been installed locally and are operating continuously. These sounders are located at Grahamstown (33.3°S, 26.5°E), Louisvale (28.5°S, 21.2°E) and Madimbo (22.4°S, 30.9°E). The addition of a modern sounder at Grahamstown enlarged the ionospheric database for this station to 30 years, making this archive a considerable asset for ionospheric research. Quality control and online availability of the data has also added to its attraction. An important requirement for empirical modelling, but especially for employing NNs, is a large database describing the history of the relationship between the ionosphere and the geophysical parameters that define its behaviour. This review describes the path of South African ionospheric modelling over the past 10 years, the role of NNs in this development, the international collaborations that have arisen from this, and the future of ionospheric modelling in South Africa.
- Full Text:
- Date Issued: 2004
- Authors: McKinnell, Lee-Anne , Poole, Allon W V
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6795 , http://hdl.handle.net/10962/d1003839
- Description: During the past decade, South African scientists have pioneered research in the field of ionospheric modelling using the technique of neural networks (NNs). Global ionospheric models have always been insufficient for the South African region owing to an historical paucity of available data. Within the past 10 years, however, three new ionospheric sounders have been installed locally and are operating continuously. These sounders are located at Grahamstown (33.3°S, 26.5°E), Louisvale (28.5°S, 21.2°E) and Madimbo (22.4°S, 30.9°E). The addition of a modern sounder at Grahamstown enlarged the ionospheric database for this station to 30 years, making this archive a considerable asset for ionospheric research. Quality control and online availability of the data has also added to its attraction. An important requirement for empirical modelling, but especially for employing NNs, is a large database describing the history of the relationship between the ionosphere and the geophysical parameters that define its behaviour. This review describes the path of South African ionospheric modelling over the past 10 years, the role of NNs in this development, the international collaborations that have arisen from this, and the future of ionospheric modelling in South Africa.
- Full Text:
- Date Issued: 2004
Neural network-based prediction techniques for global modeling of M(3000)F2 ionospheric parameter
- Oyeyemi, E O, McKinnell, Lee-Anne, Poole, Allon W V
- Authors: Oyeyemi, E O , McKinnell, Lee-Anne , Poole, Allon W V
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:6803 , http://hdl.handle.net/10962/d1004166
- Description: In recent times neural networks (NNs) have been employed to solve many problems in ionospheric predictions. This paper illustrates a new application of NNs in developing a global model of the ionospheric propagation factor M(3000)F2. NNs were trained with daily hourly values of M(3000)F2 from various ionospheric stations spanning the period 1964–1986 with the following temporal and spatial input parameters: Universal Time, geographic latitude, magnetic inclination, magnetic declination, solar zenith angle, day of the year, A16 index (a 2-day running mean of the 3-h planetary magnetic ap index), R2 index (a 2-month running mean of sunspot number), and the angle of meridian relative to the subsolar point. The performance of the NNs was verified by comparing the predicted values of M(3000)F2 with observed values from a few selected ionospheric stations and the IRI (International Reference Ionosphere) model (CCIR M(3000)F2 model) predicted values. The results obtained compared favourably with the IRI model. Based on the error differences, the result obtained justifies the potential of the NN technique for the predictions of M(3000)F2 values on a global scale.
- Full Text:
- Date Issued: 2007
- Authors: Oyeyemi, E O , McKinnell, Lee-Anne , Poole, Allon W V
- Date: 2007
- Language: English
- Type: text , Article
- Identifier: vital:6803 , http://hdl.handle.net/10962/d1004166
- Description: In recent times neural networks (NNs) have been employed to solve many problems in ionospheric predictions. This paper illustrates a new application of NNs in developing a global model of the ionospheric propagation factor M(3000)F2. NNs were trained with daily hourly values of M(3000)F2 from various ionospheric stations spanning the period 1964–1986 with the following temporal and spatial input parameters: Universal Time, geographic latitude, magnetic inclination, magnetic declination, solar zenith angle, day of the year, A16 index (a 2-day running mean of the 3-h planetary magnetic ap index), R2 index (a 2-month running mean of sunspot number), and the angle of meridian relative to the subsolar point. The performance of the NNs was verified by comparing the predicted values of M(3000)F2 with observed values from a few selected ionospheric stations and the IRI (International Reference Ionosphere) model (CCIR M(3000)F2 model) predicted values. The results obtained compared favourably with the IRI model. Based on the error differences, the result obtained justifies the potential of the NN technique for the predictions of M(3000)F2 values on a global scale.
- Full Text:
- Date Issued: 2007
Neurocognitive and symptom profiles of concussed and nonconcussed provincial rugby players over one season
- Authors: Clark, Susan Beverley
- Date: 2010
- Subjects: Rugby football injuries Brain -- Concussion -- Complications Head -- Wounds and injuries -- Complications Sports injuries -- Psychological aspects Brain damage Neuropsychological tests Rugby Union football players
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2950 , http://hdl.handle.net/10962/d1002459
- Description: Neurocognitive and symptom profiles of concussed and nonconcussed adult provincial rugby union players were investigated over one rugby season, including early season (baseline), intermittent postconcussion, and end of season testing. In a non-equivalent quasi-experimental design, nonconcussed (n = 54) and concussed (n = 17) rugby groups were compared with demographically equivalent noncontact sport controls (n = 37, and n = 17, respectively). Measures included the ImPACT cognitive and symptom composites, and the WMS-III Visual Reproduction and Verbal Paired Associates subtests. The independent and dependent comparative analyses in respect of both nonconcussed and concussed groups, provided cross-validation of poorer acute and/or chronic neuropsychological outcomes for the rugby groups on the ImPACT Reaction Time, Visual Motor Speed, Impulse Control and Symptom composites, and the WMS-III Verbal Paired Associates. The finding of significantly poorer scores on Verbal Paired Associates up to 24 days post concussion for the rugby players versus controls, was longer than the 7 – 10 day recovery period frequently cited in the literature. The overall implication of the study is that even in a group with high cognitive reserve such as these provincial level athletes, there may be prolonged acute recovery, as well as permanent deleterious neuropsychological consequences of cumulative concussive injury in association with a sport such as rugby. Accordingly, the move towards careful individualised postconcussion monitoring of neurocognitive functioning is endorsed, including early identification of any significant permanent reductions in cognitive reserve. Sensitivity of the ImPACT test might be enhanced via inclusion of a verbal associate learning task.
- Full Text:
- Date Issued: 2010
- Authors: Clark, Susan Beverley
- Date: 2010
- Subjects: Rugby football injuries Brain -- Concussion -- Complications Head -- Wounds and injuries -- Complications Sports injuries -- Psychological aspects Brain damage Neuropsychological tests Rugby Union football players
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2950 , http://hdl.handle.net/10962/d1002459
- Description: Neurocognitive and symptom profiles of concussed and nonconcussed adult provincial rugby union players were investigated over one rugby season, including early season (baseline), intermittent postconcussion, and end of season testing. In a non-equivalent quasi-experimental design, nonconcussed (n = 54) and concussed (n = 17) rugby groups were compared with demographically equivalent noncontact sport controls (n = 37, and n = 17, respectively). Measures included the ImPACT cognitive and symptom composites, and the WMS-III Visual Reproduction and Verbal Paired Associates subtests. The independent and dependent comparative analyses in respect of both nonconcussed and concussed groups, provided cross-validation of poorer acute and/or chronic neuropsychological outcomes for the rugby groups on the ImPACT Reaction Time, Visual Motor Speed, Impulse Control and Symptom composites, and the WMS-III Verbal Paired Associates. The finding of significantly poorer scores on Verbal Paired Associates up to 24 days post concussion for the rugby players versus controls, was longer than the 7 – 10 day recovery period frequently cited in the literature. The overall implication of the study is that even in a group with high cognitive reserve such as these provincial level athletes, there may be prolonged acute recovery, as well as permanent deleterious neuropsychological consequences of cumulative concussive injury in association with a sport such as rugby. Accordingly, the move towards careful individualised postconcussion monitoring of neurocognitive functioning is endorsed, including early identification of any significant permanent reductions in cognitive reserve. Sensitivity of the ImPACT test might be enhanced via inclusion of a verbal associate learning task.
- Full Text:
- Date Issued: 2010
Neurocognitive effects of head and body collisions on club level rugby union players
- Authors: Zoccola, Diana
- Date: 2015
- Subjects: Head -- Wounds and injuries -- Psychology , Neuropsychological tests , Head -- Wounds and injuries , Sports injuries -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3257 , http://hdl.handle.net/10962/d1016397
- Description: The objective of the study was to investigate the cumulative neurocognitive effects of repetitive concussive and subconcussive events in club level Rugby Union (hereafter rugby) during the course of one rugby season, in a combined group and individualized case-based approach. Amateur adult club level rugby players (n = 20) were compared with a non-contact control group (n = 22) of equivalent age, years of education and estimated IQ (p = > .05, in all instances), although the two groups were clearly differentiated on the basis of a history of reported concussions (p = < .05). Video analyses documented the tackling maneuvers observed amongst the players during all matches across the rugby season revealing a sobering average of more than a thousand tackles per player, excluding any contact practice sessions. Five rugby players (n = 5) who were observed to have a head jarring event were also isolated for individualized postconcussive follow-up analysis of their neurocognitive profiles. Measures included the ImPACT Verbal and Visual Memory, Visual Motor Speed and Reaction Time composites and the Purdue Pegboard. Independent and dependent statistical analyses were employed to compare the rugby versus control group neurocognitive test profiles at and between the three test intervals. Correlational analyses explored the association between concussion, tackling and neurocognitive test outcomes. Descriptive comparisons of individual neurocognitive test scores with normative data were employed for the case analyses. Taken together, the results implicated vulnerability amongst club rugby players on the motor and speeded tasks, with less robust indications on the memory tasks. While limited in terms of its small sample size, it is considered that the outcome of the study was rendered more robust by virtue of being methodologically multifaceted with heuristic implications for future research studies in the area. The novel inclusion of tackling data as well as fine-tuned case analyses, were of particular relevance in that regard. The results add to a growing body of literature that implicates deleterious neurocognitive effects in participants of a sport such as rugby due to repetitive head jarring incidents that are intrinsic to the game.
- Full Text:
- Date Issued: 2015
- Authors: Zoccola, Diana
- Date: 2015
- Subjects: Head -- Wounds and injuries -- Psychology , Neuropsychological tests , Head -- Wounds and injuries , Sports injuries -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3257 , http://hdl.handle.net/10962/d1016397
- Description: The objective of the study was to investigate the cumulative neurocognitive effects of repetitive concussive and subconcussive events in club level Rugby Union (hereafter rugby) during the course of one rugby season, in a combined group and individualized case-based approach. Amateur adult club level rugby players (n = 20) were compared with a non-contact control group (n = 22) of equivalent age, years of education and estimated IQ (p = > .05, in all instances), although the two groups were clearly differentiated on the basis of a history of reported concussions (p = < .05). Video analyses documented the tackling maneuvers observed amongst the players during all matches across the rugby season revealing a sobering average of more than a thousand tackles per player, excluding any contact practice sessions. Five rugby players (n = 5) who were observed to have a head jarring event were also isolated for individualized postconcussive follow-up analysis of their neurocognitive profiles. Measures included the ImPACT Verbal and Visual Memory, Visual Motor Speed and Reaction Time composites and the Purdue Pegboard. Independent and dependent statistical analyses were employed to compare the rugby versus control group neurocognitive test profiles at and between the three test intervals. Correlational analyses explored the association between concussion, tackling and neurocognitive test outcomes. Descriptive comparisons of individual neurocognitive test scores with normative data were employed for the case analyses. Taken together, the results implicated vulnerability amongst club rugby players on the motor and speeded tasks, with less robust indications on the memory tasks. While limited in terms of its small sample size, it is considered that the outcome of the study was rendered more robust by virtue of being methodologically multifaceted with heuristic implications for future research studies in the area. The novel inclusion of tackling data as well as fine-tuned case analyses, were of particular relevance in that regard. The results add to a growing body of literature that implicates deleterious neurocognitive effects in participants of a sport such as rugby due to repetitive head jarring incidents that are intrinsic to the game.
- Full Text:
- Date Issued: 2015
Neuronal nitric oxide synthase : a biomarker for Alzheimers disease : interaction of neuronal nitric oxide synthase with beta-amyloid peptides in the brain
- Authors: Padayachee, Eden Rebecca
- Date: 2011 , 2013-07-19
- Subjects: Alzheimer's disease , Nitric-oxide synthase , Biochemical markers , Amyloid beta-protein , Peptide hormones
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4086 , http://hdl.handle.net/10962/d1007677 , Alzheimer's disease , Nitric-oxide synthase , Biochemical markers , Amyloid beta-protein , Peptide hormones
- Description: High levels of the amino acid arginine and low levels of the product citrulline in the cerebrospinal fluid of Alzheimer's patients could mean that there is a decrease in the enzymes that metabolize this amino acid. One such enzyme is neuronal nitric oxide synthase (nNOS). In this study, neuronal nitric oxide synthase (nNOS), sourced from bovine brain was extracted and concentrated using two methods of precipitation: poly (ethylene glycol) 20 000 (PEG) and ammonium sulphate [(NH₄)₂S0₄). These two techniques gave no increase in yield nor fold purification and hence were abandoned in favour of ion exchange chromatography by DEAE-Sepharose. The enzyme was then successfully purified by anion-exchange and after dialysis produced a 38% yield and three fold purification and yielded the highest specific activity of 2.27 U/mg. Neuronal nitric oxide synthase (nNOS) was a heterodimeric protein with a total molecular mass of ± 225 kDa (95 and 130 kDa monomers). The temperature and pH optima of the enzyme were 40⁰C and 6.5, respectively. The kinetic parameters (KM and Vmax) of nNOS were 70 μM and 0.332 μmol.min⁻¹, respectively. Moreover neuronal nitric oxide synthase (nNOS) was relatively stable at 40⁰C (t½ = 3 h). It was also confirmed that β-amyloid peptides inhibited nNOS when bound to the enzyme and that nNOS behaved as a catalyst in fibril formation through association-dissociation between enzyme and β-amyloid peptide. It was further shown that Aβ₁₇₋₂₈ inhibited nNOS the most with a Ki of 1.92 μM and also had the highest Stern-Volmer value (Ksv) of 0.11 μM⁻¹ indicating tight binding affinity to nNOS and easier accessibility to fluor molecules during binding. Congo red, turbidity, thioflavin-T assays and transmission electron microscopy were successfully used to detect and visualize the presence of fibrils by studying the process of fibrillogenesis. Computerized molecular modeling successfully studied protein dynamics and conformational changes of nNOS. These results correlated with resonance energy transfer (FRET) results which revealed the distance of tryptophan residues from the arginine bound at enzyme active site. Both the aforementioned techniques revealed that in the natural state of the enzyme with arginine bound at the active site, the tryptophan residues (TRP₆₂₅ and TRP₇₂₁) were positioned at the surface of the enzyme 28 Å away from the active site. When the amyloid peptide (Aβ₁₇₋₂₈) was bound to the active site, these same two amino acids moved 14 Å closer to the active site. A five residue hydrophobic fragment Aβ₁₇₋₂₁ [Leu₁₇ - Val₁₈ - Phe₁₉ - Phe₂₀ - Ala₁] within Aβ₁₇₋₂₈ was shown by computer modeling to be critical to the binding of the peptide to the active site of nNOS.
- Full Text:
- Date Issued: 2011
- Authors: Padayachee, Eden Rebecca
- Date: 2011 , 2013-07-19
- Subjects: Alzheimer's disease , Nitric-oxide synthase , Biochemical markers , Amyloid beta-protein , Peptide hormones
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4086 , http://hdl.handle.net/10962/d1007677 , Alzheimer's disease , Nitric-oxide synthase , Biochemical markers , Amyloid beta-protein , Peptide hormones
- Description: High levels of the amino acid arginine and low levels of the product citrulline in the cerebrospinal fluid of Alzheimer's patients could mean that there is a decrease in the enzymes that metabolize this amino acid. One such enzyme is neuronal nitric oxide synthase (nNOS). In this study, neuronal nitric oxide synthase (nNOS), sourced from bovine brain was extracted and concentrated using two methods of precipitation: poly (ethylene glycol) 20 000 (PEG) and ammonium sulphate [(NH₄)₂S0₄). These two techniques gave no increase in yield nor fold purification and hence were abandoned in favour of ion exchange chromatography by DEAE-Sepharose. The enzyme was then successfully purified by anion-exchange and after dialysis produced a 38% yield and three fold purification and yielded the highest specific activity of 2.27 U/mg. Neuronal nitric oxide synthase (nNOS) was a heterodimeric protein with a total molecular mass of ± 225 kDa (95 and 130 kDa monomers). The temperature and pH optima of the enzyme were 40⁰C and 6.5, respectively. The kinetic parameters (KM and Vmax) of nNOS were 70 μM and 0.332 μmol.min⁻¹, respectively. Moreover neuronal nitric oxide synthase (nNOS) was relatively stable at 40⁰C (t½ = 3 h). It was also confirmed that β-amyloid peptides inhibited nNOS when bound to the enzyme and that nNOS behaved as a catalyst in fibril formation through association-dissociation between enzyme and β-amyloid peptide. It was further shown that Aβ₁₇₋₂₈ inhibited nNOS the most with a Ki of 1.92 μM and also had the highest Stern-Volmer value (Ksv) of 0.11 μM⁻¹ indicating tight binding affinity to nNOS and easier accessibility to fluor molecules during binding. Congo red, turbidity, thioflavin-T assays and transmission electron microscopy were successfully used to detect and visualize the presence of fibrils by studying the process of fibrillogenesis. Computerized molecular modeling successfully studied protein dynamics and conformational changes of nNOS. These results correlated with resonance energy transfer (FRET) results which revealed the distance of tryptophan residues from the arginine bound at enzyme active site. Both the aforementioned techniques revealed that in the natural state of the enzyme with arginine bound at the active site, the tryptophan residues (TRP₆₂₅ and TRP₇₂₁) were positioned at the surface of the enzyme 28 Å away from the active site. When the amyloid peptide (Aβ₁₇₋₂₈) was bound to the active site, these same two amino acids moved 14 Å closer to the active site. A five residue hydrophobic fragment Aβ₁₇₋₂₁ [Leu₁₇ - Val₁₈ - Phe₁₉ - Phe₂₀ - Ala₁] within Aβ₁₇₋₂₈ was shown by computer modeling to be critical to the binding of the peptide to the active site of nNOS.
- Full Text:
- Date Issued: 2011
Neuropharmacological interactions in the rat pineal gland a study of antidepressant drugs
- Authors: Banoo, Shabir
- Date: 1992
- Subjects: Antidepressants -- Research , Pineal gland -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3744 , http://hdl.handle.net/10962/d1003222 , Antidepressants -- Research , Pineal gland -- Research
- Description: The rat pineal gland provides a convenient model for investigating nor adrenergic receptor neurotransmission and the effects of various drugs on these processes in health and disease. The effect of a variety of antidepressant drugs on rat pineal gland function following acute and chronic administration is described. Antidepressants from several different classes increase melatonin synthesis in rat pineal gland cultures when administered acutely. This effect appears to be mediated by noradrenaline acting on postsynaptic β-adrenoceptors. Activation of these receptors, in turn, activates the enzyme serotonin N-acetyltransferase via a cyclic adenosine monophosphate (cAMP) second messenger system. Serotonin N-acetyltransferase catalyses the rate-limiting conversion of serotonin to melatonin. Blockade of postsynaptic β-adrenoceptors prevents the antidepressant-induced increase in melatonin synthesis. The possibility that atypical antidepressants as well as those that selectively inhibit serotonin reuptake may increase melatonin synthesis via a β-adrenoceptor mechanism is discussed. In contrast, however, antidepressants from different classes have variable effects on rat pineal gland function when administered repeatedly. Chronic treatment with antidepressants that selectively inhibit noradrenaline reuptake appear to down-regulate the β-adrenoceptor system while, simultaneously, increasing melatonin output. Atypical antidepressants and those that selectively inhibit serotonin reuptake appear to be without these effects when administered repeatedly. The pineal gland of normal rats may therefore not represent a suitable model for evaluating the biochemical effects of chronic antidepressant treatment. In an attempt to investigatc pineal gland function in rats with "model depression" , antidepressants were administered to chronically reserpinized rats. Treatment with reserpine produced an increase in the density of pineal β-adrenoceptors. In addition, pineal cyclic AMP accumulation and N-acetyltransferase activity were increased in reserpinized rats following exogenous catecholamine stimulation. Reserpine, by depleting intraneuronal catecholamine stores, prevented the nocturnal induction of N-acetyltransferase activity and reduced the synthesis of melatonin in pineal gland cultures. A variety of antidepressants, irrespective of their acute pharmacological actions, reversed these effects when administered chronically to resepinized rats. Acute antidepressant administration was not associated with a reversal of the reserpine-induced effects. These findings provide additional evidence against the hypothesis that antidepressant drugs act by reducing noradrenergic neurotransmission and casts doubt on the importance of β-adrenoceptor down-regulation in the mechanism of antidepressant action. The possibility that the pineal gland of the reserpinized rat may represent an alternative model for evaluating antidepressant therapies is discussed.
- Full Text:
- Date Issued: 1992
- Authors: Banoo, Shabir
- Date: 1992
- Subjects: Antidepressants -- Research , Pineal gland -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3744 , http://hdl.handle.net/10962/d1003222 , Antidepressants -- Research , Pineal gland -- Research
- Description: The rat pineal gland provides a convenient model for investigating nor adrenergic receptor neurotransmission and the effects of various drugs on these processes in health and disease. The effect of a variety of antidepressant drugs on rat pineal gland function following acute and chronic administration is described. Antidepressants from several different classes increase melatonin synthesis in rat pineal gland cultures when administered acutely. This effect appears to be mediated by noradrenaline acting on postsynaptic β-adrenoceptors. Activation of these receptors, in turn, activates the enzyme serotonin N-acetyltransferase via a cyclic adenosine monophosphate (cAMP) second messenger system. Serotonin N-acetyltransferase catalyses the rate-limiting conversion of serotonin to melatonin. Blockade of postsynaptic β-adrenoceptors prevents the antidepressant-induced increase in melatonin synthesis. The possibility that atypical antidepressants as well as those that selectively inhibit serotonin reuptake may increase melatonin synthesis via a β-adrenoceptor mechanism is discussed. In contrast, however, antidepressants from different classes have variable effects on rat pineal gland function when administered repeatedly. Chronic treatment with antidepressants that selectively inhibit noradrenaline reuptake appear to down-regulate the β-adrenoceptor system while, simultaneously, increasing melatonin output. Atypical antidepressants and those that selectively inhibit serotonin reuptake appear to be without these effects when administered repeatedly. The pineal gland of normal rats may therefore not represent a suitable model for evaluating the biochemical effects of chronic antidepressant treatment. In an attempt to investigatc pineal gland function in rats with "model depression" , antidepressants were administered to chronically reserpinized rats. Treatment with reserpine produced an increase in the density of pineal β-adrenoceptors. In addition, pineal cyclic AMP accumulation and N-acetyltransferase activity were increased in reserpinized rats following exogenous catecholamine stimulation. Reserpine, by depleting intraneuronal catecholamine stores, prevented the nocturnal induction of N-acetyltransferase activity and reduced the synthesis of melatonin in pineal gland cultures. A variety of antidepressants, irrespective of their acute pharmacological actions, reversed these effects when administered chronically to resepinized rats. Acute antidepressant administration was not associated with a reversal of the reserpine-induced effects. These findings provide additional evidence against the hypothesis that antidepressant drugs act by reducing noradrenergic neurotransmission and casts doubt on the importance of β-adrenoceptor down-regulation in the mechanism of antidepressant action. The possibility that the pineal gland of the reserpinized rat may represent an alternative model for evaluating antidepressant therapies is discussed.
- Full Text:
- Date Issued: 1992
Neuropharmacological profile and chemical analysis of fresh rhizome essential oil of Curcuma longa (turmeric) cultivated in Southwest Nigeria
- Idris A. Oyemitana, Christianah A. Elusiyanb, Ayoola O. Onifadea, Moses A. Akanmua, Adebola O. Oyedejic, Armando G. McDonaldd
- Authors: Idris A. Oyemitana , Christianah A. Elusiyanb , Ayoola O. Onifadea , Moses A. Akanmua , Adebola O. Oyedejic , Armando G. McDonaldd
- Date: 2017
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/3698 , vital:43928
- Full Text:
- Authors: Idris A. Oyemitana , Christianah A. Elusiyanb , Ayoola O. Onifadea , Moses A. Akanmua , Adebola O. Oyedejic , Armando G. McDonaldd
- Date: 2017
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/3698 , vital:43928
- Full Text:
Neuropharmacological profile and chemical analysis of fresh rhizome essential oil of Curcuma longa (turmeric) cultivated in Southwest Nigeria
- Idris A. Oyemitan, Adebola O. Oyedeji
- Authors: Idris A. Oyemitan , Adebola O. Oyedeji
- Date: 2017
- Language: English
- Type: Journal
- Identifier: http://hdl.handle.net/11260/5558 , vital:44604
- Full Text:
- Authors: Idris A. Oyemitan , Adebola O. Oyedeji
- Date: 2017
- Language: English
- Type: Journal
- Identifier: http://hdl.handle.net/11260/5558 , vital:44604
- Full Text:
Neuroprotective mechanisms of nevirapine and efavirenz in a model of neurodegeneration
- Authors: Zheve, Georgina Teurai
- Date: 2008
- Subjects: HIV infections -- Treatment AIDS (Disease) -- Treatment AIDS dementia complex -- Treatment Nervous system -- Degeneration -- Treatment Melatonin Neurotoxic agents Quinolinic acid
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: vital:3807 , http://hdl.handle.net/10962/d1003285
- Description: AIDS Dementia Complex (ADC) is a neurodegenerative disorder implicated in HIV-1 infection that is associated with elevated levels of the neurotoxin, quinolinic acid (QA) which causes a cascade of events to occur, leading to the production of reactive oxygen species (ROS), these being ultimately responsible for oxidative neurotoxicity. In clinical studies, Non-nucleoside reverse transcriptase inhibitors (NNRTIs), efavirenz (EFV) and nevirapine (NVP) have been shown to potentially delay the progressive degeneration of neurons, thus reducing the frequency and neurological deficits associated with ADC. Despite these neuroprotective implications, there is still no biochemical data to demonstrate the mechanisms through which these agents offer neuroprotection. The present study aims to elucidate and further characterize the possible antioxidant and neuroprotective mechanisms of NVP and EFV in vitro and in vivo, using QA-induced neurotoxicity as a model. Research has demonstrated that antioxidants and metal chelators have the ability to offer neuroprotection against free radical induced injury and may be beneficial in the prevention or treatment of neurodegeneration. Hence the antioxidant and metal binding properties of these agents were investigated respectively. Inorganic studies, including the 1, 1-diphenyl-2 picrylhydrazyl (DPPH) assay, show that these agents readily scavenge free radicals in vitro, thus postulating the antioxidant property of these agents. The enhancement of superoxide radical generation and iron mediated Fenton reaction by QA is related to lipid peroxidation in biological systems, the extent of which was assayed using the nitroblue tetrazolium and thiobarbituric acid method respectively. Both agents significantly curtail QA-induced lipid peroxidation and potentially scavenge superoxide anions generated by cyanide in vitro. Furthermore, in vivo results demonstrate the ability of NVP and EFV to protect hippocampal neurons against lipid peroxidation induced by QA and superoxide radicals generated as a consequence thereof. The alleviation of QA-induced oxidative stress in vitro possibly occurs through the binding of iron (II) and / or iron (III), and this argument is further strengthened by the ability of EFV and not NVP to reduce iron (II)-induced lipid peroxidation in vitro directly. In addition the ferrozine and electrochemistry assay were used to measure the extent of iron (II) Fe[superscript 2+] and iron (III) Fe[superscript 3+] chelation activity. Both assays demonstrate that these agents bind iron (II) and iron (III), and prevent redox recycling of iron and subsequent complexation of Fe[superscript 2+] with QA which enhances neuronal damage. Both NNRTIs inhibit the endogenous biosynthesis of QA by inhibiting liver tryptophan 2, 3-dioxygenase activity in vivo and subsequently increasing hippocampal serotonin levels. Furthermore, these agents reduce the turnover of hippocampal serotonin to 5-hydroxyindole acetic acid. NVP and not EFV increase 5-hydroxyindole acetic acid and norepinephrine levels in the hippocampus. The results of the pineal indole metabolism study show that NVP increases the synthesis of melatonin, but decreases N-acetylserotonin, 5-hydroxyindole acetic acid and 5-hydroxytryptophol levels. Furthermore, it shows that EFV decreases 5-hydroxyindole acetic acid and melatonin synthesis. Behavioural studies using a Morris water maze show that the post-treatment of rats with NVP and EFV significantly improves QA-induced spatial memory deficits in the hippocampus. This study therefore provides novel information regarding the neuroprotective mechanisms of NVP and EFV. These findings strengthen the argument that these NNRTIs not only have antiviral effects but possess potential neuroprotective properties, which may contribute to the effectiveness of these drugs in the treatment of ADC.
- Full Text:
- Date Issued: 2008
- Authors: Zheve, Georgina Teurai
- Date: 2008
- Subjects: HIV infections -- Treatment AIDS (Disease) -- Treatment AIDS dementia complex -- Treatment Nervous system -- Degeneration -- Treatment Melatonin Neurotoxic agents Quinolinic acid
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: vital:3807 , http://hdl.handle.net/10962/d1003285
- Description: AIDS Dementia Complex (ADC) is a neurodegenerative disorder implicated in HIV-1 infection that is associated with elevated levels of the neurotoxin, quinolinic acid (QA) which causes a cascade of events to occur, leading to the production of reactive oxygen species (ROS), these being ultimately responsible for oxidative neurotoxicity. In clinical studies, Non-nucleoside reverse transcriptase inhibitors (NNRTIs), efavirenz (EFV) and nevirapine (NVP) have been shown to potentially delay the progressive degeneration of neurons, thus reducing the frequency and neurological deficits associated with ADC. Despite these neuroprotective implications, there is still no biochemical data to demonstrate the mechanisms through which these agents offer neuroprotection. The present study aims to elucidate and further characterize the possible antioxidant and neuroprotective mechanisms of NVP and EFV in vitro and in vivo, using QA-induced neurotoxicity as a model. Research has demonstrated that antioxidants and metal chelators have the ability to offer neuroprotection against free radical induced injury and may be beneficial in the prevention or treatment of neurodegeneration. Hence the antioxidant and metal binding properties of these agents were investigated respectively. Inorganic studies, including the 1, 1-diphenyl-2 picrylhydrazyl (DPPH) assay, show that these agents readily scavenge free radicals in vitro, thus postulating the antioxidant property of these agents. The enhancement of superoxide radical generation and iron mediated Fenton reaction by QA is related to lipid peroxidation in biological systems, the extent of which was assayed using the nitroblue tetrazolium and thiobarbituric acid method respectively. Both agents significantly curtail QA-induced lipid peroxidation and potentially scavenge superoxide anions generated by cyanide in vitro. Furthermore, in vivo results demonstrate the ability of NVP and EFV to protect hippocampal neurons against lipid peroxidation induced by QA and superoxide radicals generated as a consequence thereof. The alleviation of QA-induced oxidative stress in vitro possibly occurs through the binding of iron (II) and / or iron (III), and this argument is further strengthened by the ability of EFV and not NVP to reduce iron (II)-induced lipid peroxidation in vitro directly. In addition the ferrozine and electrochemistry assay were used to measure the extent of iron (II) Fe[superscript 2+] and iron (III) Fe[superscript 3+] chelation activity. Both assays demonstrate that these agents bind iron (II) and iron (III), and prevent redox recycling of iron and subsequent complexation of Fe[superscript 2+] with QA which enhances neuronal damage. Both NNRTIs inhibit the endogenous biosynthesis of QA by inhibiting liver tryptophan 2, 3-dioxygenase activity in vivo and subsequently increasing hippocampal serotonin levels. Furthermore, these agents reduce the turnover of hippocampal serotonin to 5-hydroxyindole acetic acid. NVP and not EFV increase 5-hydroxyindole acetic acid and norepinephrine levels in the hippocampus. The results of the pineal indole metabolism study show that NVP increases the synthesis of melatonin, but decreases N-acetylserotonin, 5-hydroxyindole acetic acid and 5-hydroxytryptophol levels. Furthermore, it shows that EFV decreases 5-hydroxyindole acetic acid and melatonin synthesis. Behavioural studies using a Morris water maze show that the post-treatment of rats with NVP and EFV significantly improves QA-induced spatial memory deficits in the hippocampus. This study therefore provides novel information regarding the neuroprotective mechanisms of NVP and EFV. These findings strengthen the argument that these NNRTIs not only have antiviral effects but possess potential neuroprotective properties, which may contribute to the effectiveness of these drugs in the treatment of ADC.
- Full Text:
- Date Issued: 2008
Neuropsychiatric Genetics of African Populations-Psychosis (NeuroGAPPsychosis): a case-control study protocol and GWAS in Ethiopia, Kenya, South Africa and Uganda
- Stevenson, Anne, Zingela, Zukiswa, Akena, Dickens, Stroud, Rocky E, Atwoli, Lukoye, Campbell, Megan M, Chibnik, Lori B, Kwobah, Edith, Kariuki, Symon M, Martin, Alicia R, de Menil, Victoria, Newton, Charles R J C, Sibeko, Goodman, Stein, Dan J, Teferra, Solomon, Koenen, Karestan C
- Authors: Stevenson, Anne , Zingela, Zukiswa , Akena, Dickens , Stroud, Rocky E , Atwoli, Lukoye , Campbell, Megan M , Chibnik, Lori B , Kwobah, Edith , Kariuki, Symon M , Martin, Alicia R , de Menil, Victoria , Newton, Charles R J C , Sibeko, Goodman , Stein, Dan J , Teferra, Solomon , Koenen, Karestan C
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4594 , vital:44137
- Full Text:
- Authors: Stevenson, Anne , Zingela, Zukiswa , Akena, Dickens , Stroud, Rocky E , Atwoli, Lukoye , Campbell, Megan M , Chibnik, Lori B , Kwobah, Edith , Kariuki, Symon M , Martin, Alicia R , de Menil, Victoria , Newton, Charles R J C , Sibeko, Goodman , Stein, Dan J , Teferra, Solomon , Koenen, Karestan C
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4594 , vital:44137
- Full Text:
Neuru
- Chabarwa Musunda Moyo, Hugh Tracey
- Authors: Chabarwa Musunda Moyo , Hugh Tracey
- Date: 1948
- Subjects: Folk music--Africa , Folk music--Zimbabwe , Shona (African people) , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe Nedgiwe f-rh
- Language: Shona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/181534 , vital:43744 , International Library of African Music, Rhodes University, Grahamstown, South Africa , TR175-12
- Description: This old disc recording made under difficult conditions in 1948 reflects, through its technical inadequacies, something of the classical styles of Mbira playing common to Southern Rhodesian musicians. Traditional airs on Njari dza maNjanjae
- Full Text: false
- Date Issued: 1948
- Authors: Chabarwa Musunda Moyo , Hugh Tracey
- Date: 1948
- Subjects: Folk music--Africa , Folk music--Zimbabwe , Shona (African people) , Field recordings , Africa, Sub-Saharan , Africa Zimbabwe Nedgiwe f-rh
- Language: Shona
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/181534 , vital:43744 , International Library of African Music, Rhodes University, Grahamstown, South Africa , TR175-12
- Description: This old disc recording made under difficult conditions in 1948 reflects, through its technical inadequacies, something of the classical styles of Mbira playing common to Southern Rhodesian musicians. Traditional airs on Njari dza maNjanjae
- Full Text: false
- Date Issued: 1948